BLASTX nr result
ID: Coptis21_contig00021622
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00021622
(992 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003528366.1| PREDICTED: gibberellin 20 oxidase 1-like [Gl... 98 4e-28
ref|XP_003545763.1| PREDICTED: gibberellin 20 oxidase 1-like [Gl... 95 2e-27
gb|AFK47428.1| unknown [Lotus japonicus] 98 6e-27
ref|XP_004152262.1| PREDICTED: gibberellin 20 oxidase 1-B-like [... 96 8e-27
gb|AAL14683.1| 2-oxoglutarate-dependent dioxygenase [Arabidopsis... 108 9e-26
>ref|XP_003528366.1| PREDICTED: gibberellin 20 oxidase 1-like [Glycine max]
Length = 314
Score = 97.8 bits (242), Expect(2) = 4e-28
Identities = 49/100 (49%), Positives = 71/100 (71%)
Frame = +3
Query: 312 SETIHSYAKQIVELDQLLRRMNLESMGVEKYYDEHIESNFYRFRVAKYTIPNQSDELNET 491
S+TI S+++Q+ ELDQ++R+M LES+GVEKY +EH+ S Y RV KY P SD T
Sbjct: 125 SKTIQSFSEQLSELDQIIRKMILESLGVEKYLEEHMNSTNYLLRVMKYKGPQTSD----T 180
Query: 492 KMGCRANTYMNLVTILSENQVQGLEITTKDGQGVQAKVSP 611
K+G ++ N+VTIL +N+V+GLE+ TKDG+ + + SP
Sbjct: 181 KVGLTTHSDKNIVTILYQNEVEGLEVMTKDGKWISYRPSP 220
Score = 53.9 bits (128), Expect(2) = 4e-28
Identities = 27/61 (44%), Positives = 37/61 (60%)
Frame = +2
Query: 8 ELFDLPVETKSMNIKSSKRNFLACISKPPFRPHHESMSIAGVLNFEKMERFTNRMWPQGN 187
ELFDLP++TK +N+ SK+ + + + P P ESM I +E +E TN MWP GN
Sbjct: 64 ELFDLPLQTKILNV--SKKPYHGYVGQYPMVPLFESMGIDDANVYENVESMTNIMWPHGN 121
Query: 188 P 190
P
Sbjct: 122 P 122
>ref|XP_003545763.1| PREDICTED: gibberellin 20 oxidase 1-like [Glycine max]
Length = 362
Score = 95.1 bits (235), Expect(2) = 2e-27
Identities = 48/100 (48%), Positives = 70/100 (70%)
Frame = +3
Query: 312 SETIHSYAKQIVELDQLLRRMNLESMGVEKYYDEHIESNFYRFRVAKYTIPNQSDELNET 491
S+TI S+++Q+ ELDQ++R+M LES+GVEKY +EH+ S Y V KY P SD T
Sbjct: 156 SKTIQSFSEQLSELDQIIRKMILESLGVEKYLEEHMNSTNYLLGVMKYKGPQTSD----T 211
Query: 492 KMGCRANTYMNLVTILSENQVQGLEITTKDGQGVQAKVSP 611
K+G ++ N+VTIL +N+V+GLE+ TKDG+ + + SP
Sbjct: 212 KVGLTTHSDKNIVTILYQNEVEGLEVMTKDGKWISYRPSP 251
Score = 54.3 bits (129), Expect(2) = 2e-27
Identities = 27/61 (44%), Positives = 37/61 (60%)
Frame = +2
Query: 8 ELFDLPVETKSMNIKSSKRNFLACISKPPFRPHHESMSIAGVLNFEKMERFTNRMWPQGN 187
ELFDLP++TK +N+ SK+ + + + P P ESM I +E +E TN MWP GN
Sbjct: 95 ELFDLPLQTKILNV--SKKPYRGYVGQYPMVPLFESMGIDDANVYENVESMTNIMWPHGN 152
Query: 188 P 190
P
Sbjct: 153 P 153
>gb|AFK47428.1| unknown [Lotus japonicus]
Length = 311
Score = 98.2 bits (243), Expect(2) = 6e-27
Identities = 50/99 (50%), Positives = 70/99 (70%)
Frame = +3
Query: 312 SETIHSYAKQIVELDQLLRRMNLESMGVEKYYDEHIESNFYRFRVAKYTIPNQSDELNET 491
S+TI+S+++Q+ ELD ++R+M LES+GVEKY DEH+ S Y RV KY P SD T
Sbjct: 122 SKTINSFSEQLSELDHIIRKMVLESLGVEKYLDEHLHSTNYLLRVMKYKGPETSD----T 177
Query: 492 KMGCRANTYMNLVTILSENQVQGLEITTKDGQGVQAKVS 608
K+G ++ N+VTIL +NQV+GLE+ TKDG+ + K S
Sbjct: 178 KLGLTTHSDKNIVTILYQNQVEGLEVLTKDGKWISYKPS 216
Score = 49.7 bits (117), Expect(2) = 6e-27
Identities = 27/71 (38%), Positives = 39/71 (54%)
Frame = +2
Query: 8 ELFDLPVETKSMNIKSSKRNFLACISKPPFRPHHESMSIAGVLNFEKMERFTNRMWPQGN 187
ELF+LP++TK +N+ SK+ + + + P P ESM I EK+E N +WP GN
Sbjct: 61 ELFNLPLQTKLLNV--SKKPYHGYVGQYPMVPLFESMGIDDANVSEKVEAMANILWPDGN 118
Query: 188 PRVR*MYNTLS 220
P N+ S
Sbjct: 119 PSFSKTINSFS 129
>ref|XP_004152262.1| PREDICTED: gibberellin 20 oxidase 1-B-like [Cucumis sativus]
gi|449484307|ref|XP_004156846.1| PREDICTED: gibberellin
20 oxidase 1-B-like [Cucumis sativus]
Length = 314
Score = 95.5 bits (236), Expect(2) = 8e-27
Identities = 47/100 (47%), Positives = 71/100 (71%)
Frame = +3
Query: 312 SETIHSYAKQIVELDQLLRRMNLESMGVEKYYDEHIESNFYRFRVAKYTIPNQSDELNET 491
S+ + +Y++Q+ ELD+++RRM LES+GVEKY +EH+ES Y RV KY ++ ET
Sbjct: 123 SKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKY---KGTESNYET 179
Query: 492 KMGCRANTYMNLVTILSENQVQGLEITTKDGQGVQAKVSP 611
K+G ++T N+VTIL +N VQGL++ TKDG+ + + SP
Sbjct: 180 KIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSP 219
Score = 52.0 bits (123), Expect(2) = 8e-27
Identities = 28/71 (39%), Positives = 41/71 (57%)
Frame = +2
Query: 8 ELFDLPVETKSMNIKSSKRNFLACISKPPFRPHHESMSIAGVLNFEKMERFTNRMWPQGN 187
ELF+LP+ETK N+ S++ F + + P P ESM + +K++ FTN +WPQGN
Sbjct: 62 ELFNLPLETKLRNV--SQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGN 119
Query: 188 PRVR*MYNTLS 220
P + T S
Sbjct: 120 PTFSKVMETYS 130
>gb|AAL14683.1| 2-oxoglutarate-dependent dioxygenase [Arabidopsis thaliana]
Length = 320
Score = 108 bits (271), Expect(2) = 9e-26
Identities = 53/119 (44%), Positives = 82/119 (68%)
Frame = +3
Query: 309 LSETIHSYAKQIVELDQLLRRMNLESMGVEKYYDEHIESNFYRFRVAKYTIPNQSDELNE 488
+SETIH +++Q+VELD ++RRM +ES G+EKY DEH+ S +Y R+ KYT P D+ E
Sbjct: 126 ISETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEE 185
Query: 489 TKMGCRANTYMNLVTILSENQVQGLEITTKDGQGVQAKVSPKIIQTQPIIIWLNICWLL 665
TK+G R++T N++TIL + QV GLE+ TKD + ++ K S + +++ ++C LL
Sbjct: 186 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVL---VMVGDSLCALL 241
Score = 35.0 bits (79), Expect(2) = 9e-26
Identities = 20/64 (31%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
Frame = +2
Query: 8 ELFDLPVETKSMNIKSSKRNFLACISKPPFRPHHESMSIAGVLNFEKMERFTNRMWP-QG 184
+LF+LP+ TK N+ S + C + +ES+ I EK+ FT ++WP G
Sbjct: 69 DLFELPIPTKQRNVSSKPFHGYLCHNL------YESLGINDANVLEKVNDFTQQLWPDHG 122
Query: 185 NPRV 196
N +
Sbjct: 123 NKSI 126