BLASTX nr result
ID: Coptis21_contig00019368
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00019368
(1712 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABK25130.1| unknown [Picea sitchensis] 465 e-128
ref|XP_004169401.1| PREDICTED: cytochrome P450 734A1-like [Cucum... 420 e-115
ref|XP_004139422.1| PREDICTED: cytochrome P450 734A1-like [Cucum... 420 e-115
ref|XP_003609250.1| Cytochrome P450 [Medicago truncatula] gi|355... 419 e-115
dbj|BAF56240.1| cytochrome P450 enzyme [Pisum sativum] 417 e-114
>gb|ABK25130.1| unknown [Picea sitchensis]
Length = 517
Score = 465 bits (1197), Expect = e-128
Identities = 227/503 (45%), Positives = 333/503 (66%), Gaps = 12/503 (2%)
Frame = -1
Query: 1640 VNKFLVFAWWKPRKIEKQFRNQGINGPPYNLLVGSFRELAKIYSSKAKTDVVVQPLGSHM 1461
V + + WWKP +++K F +QG+ GPPY LL G+F ++ ++ + T +
Sbjct: 19 VLRIVTVIWWKPLQVKKCFESQGVRGPPYRLLNGNFADMVRMNTQAKSTPIP-------W 71
Query: 1460 KHTIVQDLLPHLHLWREKYGRVFLYWFGPSPRVVVSEPELIKEMLNNTNTYFKSIP-HPA 1284
H IV +LP+ H W + YG+ F+YW G PR+ V PELIKE+L+N ++++I +P
Sbjct: 72 SHDIVPRVLPYYHHWTKTYGKDFIYWVGSKPRLNVPHPELIKEILSNKFGHYENISSNPL 131
Query: 1283 VYDMVGDGLIFAEGDKWARHRRILSPFFTTEALKGYVHTIVSSTNEMIKRWEESINSDAP 1104
+VG GL+ G+KW +HRRI++P F + LKG V TIV ST M+++W + + S A
Sbjct: 132 GRQLVGQGLVGLRGEKWVQHRRIINPAFHLDFLKGMVPTIVESTANMLEKWGKLVLSGAE 191
Query: 1103 EFEVHSQFRTLTKNIIARTAFQSNFKQVDRIVELQIQQMAMLTQALRTFYIPGSRFLPTP 924
E EV +F LT ++I+RTA S++ + I LQ QQM + + + + Y+PG RFLPT
Sbjct: 192 EVEVLKEFCNLTADVISRTALGSSYAEAKHIFNLQDQQMLLTAELVLSVYVPGFRFLPTR 251
Query: 923 FNIKSWRVRRELEEMFRELIKSRDESIQ-------DNDLLGLMLSATNT---GNDSRKKM 774
N + W++ +E+ + R++I++R+ + DLLGLM+SA N G +M
Sbjct: 252 KNRQRWKLEKEIRKCMRQVIEARERTADIEQSGSYGTDLLGLMMSAKNKRVGGKLQDVRM 311
Query: 773 SSKELIDECRTFFVAGFETTSLLVTWAFILMGIHTDWQDKARNEVEQVL-QGKLPDYETL 597
+++E+IDEC+TF+ AG ETTS+L+TW IL+G+H DWQD+ R EV +V + +PD +++
Sbjct: 312 TTEEIIDECKTFYFAGHETTSILLTWTIILLGMHQDWQDRGRKEVLEVCGKNVVPDADSV 371
Query: 596 GRLKIVNMILNETLRLYSPAPFIMKTVEKEVELGNLTIPAGTNLEIPLIAVHHDPHQWGN 417
LKIV MILNE LRLY PA F+ + K ++LG L+IPAGT L +P++A+HHD WGN
Sbjct: 372 NHLKIVGMILNEALRLYPPAVFLQRQAVKPMQLGRLSIPAGTQLLLPILAIHHDQCLWGN 431
Query: 416 DVDEFNPDRFAEGVAKASTHPMAFMPFTSGTRVCIGMNFSMMEAKIALSMILQKFSFVIS 237
D +EFNP RF+EG+AKA HP+AFMPF G R+C+G NF+++EAK+ L+MILQ+FSFV S
Sbjct: 432 DANEFNPARFSEGIAKAVKHPLAFMPFGFGPRICVGQNFALLEAKVVLAMILQRFSFVTS 491
Query: 236 PSYKHSPDCLVTTSPMHGAHIIL 168
PSY H+P +VT P HGA +IL
Sbjct: 492 PSYAHAPVMVVTVRPQHGAQVIL 514
>ref|XP_004169401.1| PREDICTED: cytochrome P450 734A1-like [Cucumis sativus]
Length = 520
Score = 420 bits (1080), Expect = e-115
Identities = 216/502 (43%), Positives = 325/502 (64%), Gaps = 8/502 (1%)
Frame = -1
Query: 1640 VNKFLVFAWWKPRKIEKQFRNQGINGPPYNLLVGSFRELAKIYSSKAKTDVVVQPLGSHM 1461
V + +V WW+PR+IE+ F QGI GPPY +G+ +EL + KA +D +
Sbjct: 15 VLRAVVVLWWRPRRIEQHFFRQGIRGPPYRFFIGNVKELVGMMI-KASSDHSFP----NS 69
Query: 1460 KHTIVQDLLPHLHLWREKYGRVFLYWFGPSPRVVVSEPELIKEMLNNTNTYF-KSIPHPA 1284
H I+ +LP H W++ YG FL WFGP+ R+ VS+P+LI+E+ + + + K+ PHP
Sbjct: 70 SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPL 129
Query: 1283 VYDMVGDGLIFAEGDKWARHRRILSPFFTTEALKGYVHTIVSSTNEMIKRWEE---SINS 1113
V + GDGL+ +G KWA HR+I+SP F + LK + + S +M+++W S +S
Sbjct: 130 VKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSALMTSADS 189
Query: 1112 DAPEFEVHSQFRTLTKNIIARTAFQSNFKQVDRIVELQIQQMAMLTQALRTFYIPGSRFL 933
D E EV F+TLT+++I RTAF S+++ I LQ QQM + +QA +IPG RFL
Sbjct: 190 DEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFL 249
Query: 932 PTPFNIKSWRVRRELEEMFRELI-KSRDESIQDN--DLLGLMLSATNTGNDSRKKMSSKE 762
PT N+ SWR+ +E+ + +LI + R+ SI+ + DLLGLM+ A+ + S ++ +
Sbjct: 250 PTRTNVNSWRLDKEIRKSLMKLIDRRRENSIETSSKDLLGLMIRASKSSPSST--ITVND 307
Query: 761 LIDECRTFFVAGFETTSLLVTWAFILMGIHTDWQDKARNEVEQVLQGK-LPDYETLGRLK 585
+++EC+ FF AG +TTS L+TW ILM +H WQ +AR+EV + + +P + + +LK
Sbjct: 308 IVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQARDEVLRECGARDIPSKDDVTKLK 367
Query: 584 IVNMILNETLRLYSPAPFIMKTVEKEVELGNLTIPAGTNLEIPLIAVHHDPHQWGNDVDE 405
+++MI+NE+LRLY P ++ + +VELG +P GT L IP++AVHHD WGNDV+E
Sbjct: 368 MLSMIINESLRLYPPTVATIRQAKVDVELGGYMLPRGTELLIPILAVHHDQTIWGNDVNE 427
Query: 404 FNPDRFAEGVAKASTHPMAFMPFTSGTRVCIGMNFSMMEAKIALSMILQKFSFVISPSYK 225
FNP RFAEGVAKA+ H + F+PF G R CIG N ++++AK+AL++ILQ+FSF + PSY+
Sbjct: 428 FNPARFAEGVAKAANHRVGFIPFGLGARTCIGQNLAILQAKLALALILQRFSFRLGPSYQ 487
Query: 224 HSPDCLVTTSPMHGAHIILRRI 159
HSP + P HGA II +++
Sbjct: 488 HSPAVQMLLYPRHGAPIIFKKL 509
>ref|XP_004139422.1| PREDICTED: cytochrome P450 734A1-like [Cucumis sativus]
Length = 520
Score = 420 bits (1080), Expect = e-115
Identities = 216/502 (43%), Positives = 325/502 (64%), Gaps = 8/502 (1%)
Frame = -1
Query: 1640 VNKFLVFAWWKPRKIEKQFRNQGINGPPYNLLVGSFRELAKIYSSKAKTDVVVQPLGSHM 1461
V + +V WW+PR+IE+ F QGI GPPY +G+ +EL + KA +D +
Sbjct: 15 VLRAVVVLWWRPRRIEQHFFRQGIRGPPYRFFIGNVKELVGMMI-KASSDHSFP----NS 69
Query: 1460 KHTIVQDLLPHLHLWREKYGRVFLYWFGPSPRVVVSEPELIKEMLNNTNTYF-KSIPHPA 1284
H I+ +LP H W++ YG FL WFGP+ R+ VS+P+LI+E+ + + + K+ PHP
Sbjct: 70 SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPL 129
Query: 1283 VYDMVGDGLIFAEGDKWARHRRILSPFFTTEALKGYVHTIVSSTNEMIKRWEE---SINS 1113
V + GDGL+ +G KWA HR+I+SP F + LK + + S +M+++W S +S
Sbjct: 130 VKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSALMTSADS 189
Query: 1112 DAPEFEVHSQFRTLTKNIIARTAFQSNFKQVDRIVELQIQQMAMLTQALRTFYIPGSRFL 933
D E EV F+TLT+++I RTAF S+++ I LQ QQM + +QA +IPG RFL
Sbjct: 190 DEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFL 249
Query: 932 PTPFNIKSWRVRRELEEMFRELI-KSRDESIQDN--DLLGLMLSATNTGNDSRKKMSSKE 762
PT N+ SWR+ +E+ + +LI + R+ SI+ + DLLGLM+ A+ + S ++ +
Sbjct: 250 PTRTNVNSWRLDKEIRKSLMKLIDRRRENSIETSSKDLLGLMIRASKSSPSST--ITVND 307
Query: 761 LIDECRTFFVAGFETTSLLVTWAFILMGIHTDWQDKARNEVEQVLQGK-LPDYETLGRLK 585
+++EC+ FF AG +TTS L+TW ILM +H WQ +AR+EV + + +P + + +LK
Sbjct: 308 IVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQARDEVLRECGARDIPSKDDVTKLK 367
Query: 584 IVNMILNETLRLYSPAPFIMKTVEKEVELGNLTIPAGTNLEIPLIAVHHDPHQWGNDVDE 405
+++MI+NE+LRLY P ++ + +VELG +P GT L IP++AVHHD WGNDV+E
Sbjct: 368 MLSMIINESLRLYPPTVATIRQAKVDVELGGYMLPRGTELLIPILAVHHDQTIWGNDVNE 427
Query: 404 FNPDRFAEGVAKASTHPMAFMPFTSGTRVCIGMNFSMMEAKIALSMILQKFSFVISPSYK 225
FNP RFAEGVAKA+ H + F+PF G R CIG N ++++AK+AL++ILQ+FSF + PSY+
Sbjct: 428 FNPARFAEGVAKAANHRVGFIPFGLGARTCIGQNLAILQAKLALALILQRFSFRLGPSYQ 487
Query: 224 HSPDCLVTTSPMHGAHIILRRI 159
HSP + P HGA II +++
Sbjct: 488 HSPAVQMLLYPRHGAPIIFKKL 509
>ref|XP_003609250.1| Cytochrome P450 [Medicago truncatula] gi|355510305|gb|AES91447.1|
Cytochrome P450 [Medicago truncatula]
Length = 530
Score = 419 bits (1078), Expect = e-115
Identities = 210/506 (41%), Positives = 327/506 (64%), Gaps = 12/506 (2%)
Frame = -1
Query: 1640 VNKFLVFAWWKPRKIEKQFRNQGINGPPYNLLVGSFRELAKIYSSKAKTDVVVQPLGSHM 1461
V K V WW+PRKIE F QGI GPPY+ +G+ +EL + + QP+ +
Sbjct: 22 VLKVTVLLWWRPRKIEGYFAKQGIRGPPYHFFIGNVKELVGMMLKASS-----QPM-PNF 75
Query: 1460 KHTIVQDLLPHLHLWREKYGRVFLYWFGPSPRVVVSEPELIKEMLNNTNTYF-KSIPHPA 1284
H I+ +L H W++ YG +FL WFGP+ R+ VS+P+LI+E+ + + ++ K+ P
Sbjct: 76 SHNILPRVLSFYHHWKKIYGGMFLVWFGPTVRLTVSDPDLIREIFTSKSEFYEKNEAPPL 135
Query: 1283 VYDMVGDGLIFAEGDKWARHRRILSPFFTTEALKGYVHTIVSSTNEMIKRWEESINSDAP 1104
V + GDGL+ +G+KWA HR+I+SP F E LK + + +S EM+++W E +
Sbjct: 136 VKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLIPVMATSVVEMLEKWSEMSDKGEV 195
Query: 1103 EFEVHSQFRTLTKNIIARTAFQSNFKQVDRIVELQIQQMAMLTQALRTFYIPGSRFLPTP 924
E EV F+TLT+++I +TAF S+++ I LQ QQM + A + +IPG RF PT
Sbjct: 196 EIEVSEWFQTLTEDVITKTAFGSSYQDGKAIFHLQAQQMILAADAFQKIFIPGYRFFPTR 255
Query: 923 FNIKSWRVRRELEEMFRELIKSRDESIQDN----------DLLGLMLSATNTGNDSRKKM 774
NIKSW++ +E+++ +LIK R+E++ + DLLGLM+ A++ N++ +
Sbjct: 256 KNIKSWKLDKEIKKSLVKLIKRREENLNNGNEERIEKGPKDLLGLMIEASSNNNNTN--V 313
Query: 773 SSKELIDECRTFFVAGFETTSLLVTWAFILMGIHTDWQDKARNEVEQVLQGK-LPDYETL 597
+ ++++EC++FF AG +TTS L+TW IL+ +H WQ +AR+EV ++ + LP + +
Sbjct: 314 TVDDIVEECKSFFFAGKQTTSNLLTWTTILLAMHPQWQVQARDEVLKMCGSRDLPTKDHV 373
Query: 596 GRLKIVNMILNETLRLYSPAPFIMKTVEKEVELGNLTIPAGTNLEIPLIAVHHDPHQWGN 417
+LK +NMI+NE+LRLY P ++ + +V+LG IP GT L IP++AVHHD WGN
Sbjct: 374 VKLKTLNMIVNESLRLYPPTIATIRRAKTDVDLGGYKIPRGTELLIPILAVHHDIAIWGN 433
Query: 416 DVDEFNPDRFAEGVAKASTHPMAFMPFTSGTRVCIGMNFSMMEAKIALSMILQKFSFVIS 237
DV+EFNP RF+EGVA+A+ HP+AF+PF G R CIG N ++++ K+AL++ILQ+ SF ++
Sbjct: 434 DVNEFNPGRFSEGVARAAKHPVAFIPFGLGVRTCIGQNLAVLQTKLALAIILQRLSFRLA 493
Query: 236 PSYKHSPDCLVTTSPMHGAHIILRRI 159
PSY+H+P L+ P +GA II ++
Sbjct: 494 PSYQHAPTVLMLLYPQYGAPIIFNQL 519
>dbj|BAF56240.1| cytochrome P450 enzyme [Pisum sativum]
Length = 523
Score = 417 bits (1073), Expect = e-114
Identities = 212/502 (42%), Positives = 320/502 (63%), Gaps = 8/502 (1%)
Frame = -1
Query: 1640 VNKFLVFAWWKPRKIEKQFRNQGINGPPYNLLVGSFRELAKIYSSKAKTDVVVQPLGSHM 1461
V K V WW+PRKIE F QGI GPPY +G+ +EL + + QP+ +
Sbjct: 21 VLKLTVLLWWRPRKIEGYFSKQGIRGPPYRFFIGNVKELVAMMIQASS-----QPM-PNF 74
Query: 1460 KHTIVQDLLPHLHLWREKYGRVFLYWFGPSPRVVVSEPELIKEMLNNTNTYF-KSIPHPA 1284
H I+ +L H W++ YG FL WFGP+ R+ VS+P+LI+E+ + + ++ K+ P
Sbjct: 75 SHNILPRVLSFYHHWKKIYGGTFLVWFGPTVRLTVSDPDLIREIFTSKSEFYEKNEAPPL 134
Query: 1283 VYDMVGDGLIFAEGDKWARHRRILSPFFTTEALKGYVHTIVSSTNEMIKRWEESINSDAP 1104
V + GDGL+ +G+KWA HRRI+SP F E LK + + +S EM++ W E +
Sbjct: 135 VKQLEGDGLLSLKGEKWAHHRRIISPTFHMENLKLLIPVMATSVVEMLENWSEMSHKGEV 194
Query: 1103 EFEVHSQFRTLTKNIIARTAFQSNFKQVDRIVELQIQQMAMLTQALRTFYIPGSRFLPTP 924
E EV F+TLT+++I +TAF S+++ I LQ QQM + A + +IPG RF PT
Sbjct: 195 EIEVSECFQTLTEDVITKTAFGSSYQDGKAIFRLQAQQMVLAADAFQKVFIPGYRFFPTR 254
Query: 923 FNIKSWRVRRELEEMFRELIKSRDESIQDN------DLLGLMLSATNTGNDSRKKMSSKE 762
NIKSW++ +++++ +LI+ R E+ + DLLGLM+ A+N N ++ +
Sbjct: 255 RNIKSWKLDKQIKKSLVKLIERRRENSNERIEKGPKDLLGLMIQASNKTN-----VTVDD 309
Query: 761 LIDECRTFFVAGFETTSLLVTWAFILMGIHTDWQDKARNEVEQVLQGK-LPDYETLGRLK 585
++ EC++FF AG +TTS L+TW IL+ +H WQ +AR+EV ++ + +P + + +LK
Sbjct: 310 IVGECKSFFFAGKQTTSNLLTWTTILLAMHPQWQVQARDEVLKMCGSRDVPTKDHVVKLK 369
Query: 584 IVNMILNETLRLYSPAPFIMKTVEKEVELGNLTIPAGTNLEIPLIAVHHDPHQWGNDVDE 405
+NMI+NE+LRLY P ++ + +VELG IP GT L IP++AVHHD WGNDV+E
Sbjct: 370 TLNMIVNESLRLYPPTIATIRRAKTDVELGGYKIPRGTELLIPILAVHHDQAIWGNDVNE 429
Query: 404 FNPDRFAEGVAKASTHPMAFMPFTSGTRVCIGMNFSMMEAKIALSMILQKFSFVISPSYK 225
FNP RF+EGVA+A+ HP+AF+PF G R CIG N ++++ K+AL MILQ+FSF ++PSY+
Sbjct: 430 FNPGRFSEGVARAAKHPVAFIPFGLGVRTCIGQNLAVLQTKLALVMILQRFSFRLAPSYQ 489
Query: 224 HSPDCLVTTSPMHGAHIILRRI 159
H+P L+ P +GA II +++
Sbjct: 490 HAPTVLMLLYPQYGAPIIFKQL 511