BLASTX nr result
ID: Coptis21_contig00016177
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00016177
(1280 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002532421.1| acyltransferase, putative [Ricinus communis]... 303 5e-80
ref|XP_003635026.1| PREDICTED: acyl-CoA--sterol O-acyltransferas... 290 7e-76
emb|CAN64383.1| hypothetical protein VITISV_017856 [Vitis vinifera] 289 9e-76
ref|XP_002313330.1| predicted protein [Populus trichocarpa] gi|2... 288 3e-75
ref|XP_002529023.1| acyltransferase, putative [Ricinus communis]... 284 4e-74
>ref|XP_002532421.1| acyltransferase, putative [Ricinus communis]
gi|223527870|gb|EEF29962.1| acyltransferase, putative
[Ricinus communis]
Length = 369
Score = 303 bits (777), Expect = 5e-80
Identities = 160/359 (44%), Positives = 217/359 (60%), Gaps = 4/359 (1%)
Frame = -3
Query: 1257 MEGEIRGFIQAWAIVSASLTYCYFISSRIPKGKFRLLSILPIISIFAFLPLCLSSFFLQF 1078
MEG+ FI+AW ++ ASL+YC+ IPKG RL +LPI+ +F LPL +SS Q
Sbjct: 1 MEGKAHNFIKAWLLIFASLSYCFATGKIIPKGSTRLFFLLPIVCLFLILPLNISSVHFQG 60
Query: 1077 YFGPNIGWLANFKLLLFAFGKDDALSINPSTSLLHFIVAVAFPVRIKPNSSQ----RIQN 910
I WLANFKLLLFAFGK S N STS F++ P++I+ N SQ +N
Sbjct: 61 MTAFFIAWLANFKLLLFAFGKGPLSSDNSSTSFTRFVLNACLPIKIQENPSQGSHINSKN 120
Query: 909 KETPSTASTKQDSKLNIIFVINVLIWAVMVQVYANNIVFKQETLFMKPIVAQFLYSARML 730
KE P S+K+ K + I ++ A++++VY + F+ P V LY +
Sbjct: 121 KENPHLNSSKKCHKSFPNYAIKGILLALLIRVYDYHE-------FIHPNVILVLYYLHIY 173
Query: 729 FGTQVMFVLQAALAEPLIRQETEPQFNEPYLSTSLQDFWGKRWNRTVNSLLKSTIFQPTK 550
+++ + AALA +I E EPQFNEPYLSTSLQDFWG+RWN V S+L+ T++ P
Sbjct: 174 LFLELVLAMLAALARAMIGVELEPQFNEPYLSTSLQDFWGRRWNLMVTSILRPTVYDPLI 233
Query: 549 SLLTPIFGKRWTIHLAILATFVVSGLMHELMHFYVGRQWPTWEVMWFFVIHGVCLAVELE 370
+PI G+RW AIL TF VS +MHEL+ +++GR PTWE+ WFF++HGVCL E+
Sbjct: 234 RTSSPIIGRRWASLPAILGTFTVSAIMHELIFYHLGRVRPTWEITWFFMLHGVCLVAEVA 293
Query: 369 VKKKALKHGWRLHWMISLPLTVGFVVVTGIWLCYTELINFGADIREVEEYVAFINYVKN 193
KKA+ WRL +S PLT+GFV VTG WL + L+ D+R EEY A +VK+
Sbjct: 294 F-KKAVNGRWRLSRFVSTPLTIGFVAVTGFWLFFPSLLRCKTDVRAFEEYSALSEFVKS 351
>ref|XP_003635026.1| PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Vitis vinifera]
Length = 372
Score = 290 bits (741), Expect = 7e-76
Identities = 151/355 (42%), Positives = 221/355 (62%)
Frame = -3
Query: 1257 MEGEIRGFIQAWAIVSASLTYCYFISSRIPKGKFRLLSILPIISIFAFLPLCLSSFFLQF 1078
MEGE F+ W + S +YCY ++ IP G+ RLL+I+P++++F FLPL L++ L
Sbjct: 1 MEGEFNNFVMVWVLAFVSFSYCYTMAKIIPSGRARLLTIIPVMALFLFLPLRLNTLHLGG 60
Query: 1077 YFGPNIGWLANFKLLLFAFGKDDALSINPSTSLLHFIVAVAFPVRIKPNSSQRIQNKETP 898
F I WLANFKLLLFAFG + L+ +PS SLL F + P++I+ N +E P
Sbjct: 61 TFAFFIAWLANFKLLLFAFG-EGPLASDPSISLLRFAALASLPIKIQQNPPPNDPYRENP 119
Query: 897 STASTKQDSKLNIIFVINVLIWAVMVQVYANNIVFKQETLFMKPIVAQFLYSARMLFGTQ 718
S K+ K ++ + ++ AV++ V A+ F ++ M PIV+ FL +M +
Sbjct: 120 SLEKPKKGQKSPLVSTVKGVVLAVVI-VAAH---FSED---MHPIVSLFLLIFQMYLSLE 172
Query: 717 VMFVLQAALAEPLIRQETEPQFNEPYLSTSLQDFWGKRWNRTVNSLLKSTIFQPTKSLLT 538
+ L A L L+ E PQFNEPYLSTSLQDFWG+RWN +S+L+ T++ PT +
Sbjct: 173 FILALVATLPRVLLGMELAPQFNEPYLSTSLQDFWGRRWNIMASSILRPTVYLPTLHVSA 232
Query: 537 PIFGKRWTIHLAILATFVVSGLMHELMHFYVGRQWPTWEVMWFFVIHGVCLAVELEVKKK 358
+FG++W A++ATF+VS +MHEL+ +Y+GR PTW + +FFV+HG+CL EL + KK
Sbjct: 233 RLFGRKWAPIPAVMATFIVSAVMHELIFYYMGRTAPTWHISFFFVLHGLCLTFELAL-KK 291
Query: 357 ALKHGWRLHWMISLPLTVGFVVVTGIWLCYTELINFGADIREVEEYVAFINYVKN 193
L L +S PLTV FVV TG WL + +L+ AD+R +EEY AF+ ++K+
Sbjct: 292 VLGGILALPAFVSGPLTVAFVVSTGFWLFFPQLLRLKADVRAIEEYAAFVAFLKD 346
>emb|CAN64383.1| hypothetical protein VITISV_017856 [Vitis vinifera]
Length = 382
Score = 289 bits (740), Expect = 9e-76
Identities = 153/355 (43%), Positives = 219/355 (61%)
Frame = -3
Query: 1257 MEGEIRGFIQAWAIVSASLTYCYFISSRIPKGKFRLLSILPIISIFAFLPLCLSSFFLQF 1078
MEGE F+ W + S +YCY ++ IP G+ RLL+I+P++++F FLPL L++ L
Sbjct: 11 MEGEFNNFVMVWVLAFVSFSYCYTMAKIIPSGRARLLTIIPVMALFLFLPLRLNTLHLGG 70
Query: 1077 YFGPNIGWLANFKLLLFAFGKDDALSINPSTSLLHFIVAVAFPVRIKPNSSQRIQNKETP 898
F I WLANFKLLLFAFG + L+ +PS SLL F + P++I+ N +E P
Sbjct: 71 TFAFFIAWLANFKLLLFAFG-EGPLASDPSISLLRFAALASLPIKIQQNPPPNGPYRENP 129
Query: 897 STASTKQDSKLNIIFVINVLIWAVMVQVYANNIVFKQETLFMKPIVAQFLYSARMLFGTQ 718
S K+ K + + ++ A MV V A+ F ++ M PIV+ FL +M +
Sbjct: 130 SLEKPKKGQKSPXVXTVKGVVLA-MVIVAAH---FSED---MHPIVSLFLLJFQMYLSLE 182
Query: 717 VMFVLQAALAEPLIRQETEPQFNEPYLSTSLQDFWGKRWNRTVNSLLKSTIFQPTKSLLT 538
+ L A L L+ E PQFNEPYLSTSLQDFWG+RWN S+L+ T++ PT +
Sbjct: 183 FILALVATLPRVLLGMELAPQFNEPYLSTSLQDFWGRRWNIMAXSILRPTVYLPTLHVSA 242
Query: 537 PIFGKRWTIHLAILATFVVSGLMHELMHFYVGRQWPTWEVMWFFVIHGVCLAVELEVKKK 358
+FG++W A++ATF+VS +MHEL+ +Y+GR PTW + +FFV+HG+CL VEL + KK
Sbjct: 243 RLFGRKWAPIPAVMATFIVSAVMHELIFYYMGRTAPTWHISFFFVLHGLCLTVELAL-KK 301
Query: 357 ALKHGWRLHWMISLPLTVGFVVVTGIWLCYTELINFGADIREVEEYVAFINYVKN 193
L L +S PLTV FVV TG WL + +L+ AD+R +EEY AF+ ++K+
Sbjct: 302 VLGGIXALPAFVSGPLTVAFVVSTGFWLFFPQLLRLKADVRAIEEYAAFVAFLKD 356
>ref|XP_002313330.1| predicted protein [Populus trichocarpa] gi|222849738|gb|EEE87285.1|
predicted protein [Populus trichocarpa]
Length = 363
Score = 288 bits (736), Expect = 3e-75
Identities = 152/356 (42%), Positives = 217/356 (60%), Gaps = 1/356 (0%)
Frame = -3
Query: 1257 MEGEIRGFIQAWAIVSASLTYCYFISSRIPKGKFRLLSILPIISIFAFLPLCLSSFFLQF 1078
MEGEIR F++ W + L CY I IPKGK RLL ++P + +F +LPL LS+ FL
Sbjct: 1 MEGEIRNFLKVWLSILFCLCCCYAIGKAIPKGKTRLLFLIPFVCLFFYLPLNLSTIFLGG 60
Query: 1077 YFGPNIGWLANFKLLLFAFGKDDALSINPSTSLLHFIVAVAFPVRIKPNSSQRIQNKET- 901
I WL NFKLLLFA GK S S SLL F+ P++I+ N + QN+E
Sbjct: 61 NTAFYIAWLGNFKLLLFACGKGPLSSQYSSRSLLTFVSVACLPIKIQENPPPKSQNQENQ 120
Query: 900 PSTASTKQDSKLNIIFVINVLIWAVMVQVYANNIVFKQETLFMKPIVAQFLYSARMLFGT 721
P T Q K ++ + I ++WA +V ++ N ++ P + L +
Sbjct: 121 PPVIPTDQTHKSSLNYAIKGVLWAFLVVIHDYNE-------YIHPKIILCLLCLHLYIFL 173
Query: 720 QVMFVLQAALAEPLIRQETEPQFNEPYLSTSLQDFWGKRWNRTVNSLLKSTIFQPTKSLL 541
V+ + A LA ++ E EPQFNEP+LSTSLQDFWGKRWN V+S+L+ T+++PT+++
Sbjct: 174 DVVLAVVAVLARTILGLELEPQFNEPHLSTSLQDFWGKRWNLMVSSILRQTVYEPTRNIS 233
Query: 540 TPIFGKRWTIHLAILATFVVSGLMHELMHFYVGRQWPTWEVMWFFVIHGVCLAVELEVKK 361
T + G+RW+ A+LATF+ S +MHE+M +Y+ R PTWEV WFF++HG C+ VE+ V K
Sbjct: 234 TRVVGRRWSPIPAVLATFLSSAIMHEVMFYYLLRAKPTWEVTWFFLLHGFCVTVEI-VIK 292
Query: 360 KALKHGWRLHWMISLPLTVGFVVVTGIWLCYTELINFGADIREVEEYVAFINYVKN 193
KALK W+L +IS T+GFV+VT WL + + + AD+R EEY A +V++
Sbjct: 293 KALKGRWQLPRLISGLFTIGFVMVTSCWLFFPKPLQRRADVRAFEEYAALRAFVRD 348
>ref|XP_002529023.1| acyltransferase, putative [Ricinus communis]
gi|223531503|gb|EEF33334.1| acyltransferase, putative
[Ricinus communis]
Length = 359
Score = 284 bits (726), Expect = 4e-74
Identities = 149/359 (41%), Positives = 214/359 (59%), Gaps = 1/359 (0%)
Frame = -3
Query: 1257 MEGEIRGFIQAWAIVSASLTYCYFISSRIPKGKFRLLSILPIISIFAFLPLCLSSFFLQF 1078
MEGE + FI+ W + + L YCY+++S+IPKG FRL+S+LPI +F LPL L+SF L
Sbjct: 1 MEGEFKNFIKVWILATTCLCYCYYVASKIPKGVFRLISLLPIFYLFIILPLVLTSFHLGA 60
Query: 1077 YFGPNIGWLANFKLLLFAFGKDDALSINP-STSLLHFIVAVAFPVRIKPNSSQRIQNKET 901
+ WLANFKLLLF+F D ++P S L HFI P++++ N+ K
Sbjct: 61 PTTFFLIWLANFKLLLFSF---DQGPLSPASLKLFHFISLTYLPIKLRQNNDPSHTQKHQ 117
Query: 900 PSTASTKQDSKLNIIFVINVLIWAVMVQVYANNIVFKQETLFMKPIVAQFLYSARMLFGT 721
+ ++ ++ + VL+ A++ Y+ +KQ F+ P + LY M
Sbjct: 118 TAYTTSAPFLPRRVVLPLKVLLVALLFHCYS----YKQ---FLHPYLILALYCLHMYLEL 170
Query: 720 QVMFVLQAALAEPLIRQETEPQFNEPYLSTSLQDFWGKRWNRTVNSLLKSTIFQPTKSLL 541
+++ + A A L E EPQFNEPYL+TSLQDFWG+RWN V S+L T++ P +
Sbjct: 171 EIILAISATPARVLFGFELEPQFNEPYLATSLQDFWGRRWNLMVTSILHPTVYYPVRQFF 230
Query: 540 TPIFGKRWTIHLAILATFVVSGLMHELMHFYVGRQWPTWEVMWFFVIHGVCLAVELEVKK 361
+ G+ W A +ATFVVSGLMHE+++FYV R PTWEV WFF++HGVC+ +E+ +KK
Sbjct: 231 MGLIGQTWASLPAFVATFVVSGLMHEIIYFYVTRVSPTWEVTWFFIVHGVCVVIEVALKK 290
Query: 360 KALKHGWRLHWMISLPLTVGFVVVTGIWLCYTELINFGADIREVEEYVAFINYVKNTLL 184
A K W L IS+PLTV FV VT +WL + +L D + + EY F+N +K L+
Sbjct: 291 MA-KGRWELDRAISIPLTVVFVGVTAVWLFFPQLTRNRIDDKAIGEYSIFVNLLKQELM 348