BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00015945
         (1453 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      724   0.0  
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   631   e-178
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       565   e-159
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   470   e-130
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   459   e-126

>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  724 bits (1870), Expect = 0.0
 Identities = 357/358 (99%), Positives = 357/358 (99%)
 Frame = +2

Query: 131  MAVEAKQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTNEEQIAEIQNL 310
            MAVEAKQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPT EEQIAEIQNL
Sbjct: 1    MAVEAKQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNL 60

Query: 311  THSLRQMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDL 490
            THSLRQMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDL
Sbjct: 61   THSLRQMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDL 120

Query: 491  YCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLN 670
            YCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLN
Sbjct: 121  YCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLN 180

Query: 671  VEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFA 850
            VEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFA
Sbjct: 181  VEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFA 240

Query: 851  YHYEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKR 1030
            YHYEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKR
Sbjct: 241  YHYEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKR 300

Query: 1031 LDANLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 1204
            LDANLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK
Sbjct: 301  LDANLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 358


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
            gi|51980204|gb|AAU20766.1| (S)-coclaurine
            N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  631 bits (1627), Expect = e-178
 Identities = 308/361 (85%), Positives = 336/361 (93%), Gaps = 3/361 (0%)
 Frame = +2

Query: 131  MAVEAKQT--KKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTNEEQIAEIQ 304
            MAVE KQ   KKA IVELLK+LELGLVP D+IK+LIR +L RRLQWG K T EEQIA++ 
Sbjct: 1    MAVEGKQVAPKKAIIVELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLV 60

Query: 305  NLTHSLRQMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAML 484
            NLTHSLRQMKIATEVETLD Q+YE+PI+FLKIMNGSNLKGSCCYFK DSTTLDEAEIAML
Sbjct: 61   NLTHSLRQMKIATEVETLDDQMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAML 120

Query: 485  DLYCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNL 664
            +LYCERAQI+DG SVLDLGCGQGALTL+VAQKYKN RVTAVTNSVSQKE+IEEESR+RNL
Sbjct: 121  ELYCERAQIKDGHSVLDLGCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNL 180

Query: 665  LNVEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKT 844
             NVEV LADITTH+M +TYDRILV+ELFEHMKNYELLLRKI EW++KDGLLF+EHICHKT
Sbjct: 181  SNVEVLLADITTHKMPDTYDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKT 240

Query: 845  FAYHYEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWL 1024
            FAYHYEP+D+DDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWL
Sbjct: 241  FAYHYEPIDEDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWL 300

Query: 1025 KRLDANLDVIKPMFETLMGN-EEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKK 1201
            KRLDAN+++IKPMF T+ G   +EA+KLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKK
Sbjct: 301  KRLDANVELIKPMFVTITGQCRQEAMKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKK 360

Query: 1202 K 1204
            K
Sbjct: 361  K 361


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  565 bits (1457), Expect = e-159
 Identities = 266/344 (77%), Positives = 307/344 (89%)
 Frame = +2

Query: 173  ELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTNEEQIAEIQNLTHSLRQMKIATEVE 352
            EL K+LELGL+P D++K+L+R +LARRLQWGYK T EEQIA++ +LTHSLR M IA EV+
Sbjct: 8    ELFKKLELGLIPNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIAMEVD 67

Query: 353  TLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQDGQSVL 532
            TLDS +YE+P++FLKIMNGS LK S CYFK+DSTTLDEAEIAM+DL+CERAQI+DG SVL
Sbjct: 68   TLDSNMYEVPLDFLKIMNGSALKMSSCYFKDDSTTLDEAEIAMMDLHCERAQIKDGHSVL 127

Query: 533  DLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADITTHEMA 712
            DLGCG G+LT +VAQKYK   VTAVTNSVSQKEYIEEE+RRRNL NVEV LADI THEMA
Sbjct: 128  DLGCGHGSLTFYVAQKYKKSHVTAVTNSVSQKEYIEEEARRRNLSNVEVLLADIATHEMA 187

Query: 713  ETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTE 892
            +TYDRILV+ LFEHMKNY LLL+KISEW++KDGLLF++H+CHKTFA+H EP+D+DDW +E
Sbjct: 188  DTYDRILVVGLFEHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDEDDWLSE 247

Query: 893  YVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFET 1072
            Y FPAGTMIIPSASFFLYFQDD SVVNHWTLSGKHFSRT EEWLKRLDAN+ VI PMF T
Sbjct: 248  YAFPAGTMIIPSASFFLYFQDDASVVNHWTLSGKHFSRTTEEWLKRLDANVKVIIPMFAT 307

Query: 1073 LMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 1204
            + G+EEEAVKL+NYWRG C  G+E++GYNNGEEWM SHVLFKKK
Sbjct: 308  ITGSEEEAVKLLNYWRGLCFFGVELYGYNNGEEWMESHVLFKKK 351


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
            somniferum]
          Length = 351

 Score =  470 bits (1210), Expect = e-130
 Identities = 217/344 (63%), Positives = 278/344 (80%)
 Frame = +2

Query: 173  ELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTNEEQIAEIQNLTHSLRQMKIATEVE 352
            ELL+ +ELGL+P  +I+QLIR EL +RLQWGYK T+EEQ++++ +L HSL+ MK+ATE+E
Sbjct: 8    ELLRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEME 67

Query: 353  TLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQDGQSVL 532
             LD +LYE P+EFLKI +GSN+K S  Y+ ++STTLDEAEIAMLDLY ERAQI+DGQSVL
Sbjct: 68   NLDLKLYEAPMEFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVL 127

Query: 533  DLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADITTHEMA 712
            DLGCG GA+ L  A K+K C+ T VT+SV QK+YIE + +   L NV+V LADITT+E  
Sbjct: 128  DLGCGLGAVALFGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADITTYETE 187

Query: 713  ETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTE 892
            E +DRI  +EL EHMKNY+LLL+KISEW+  DGLLF+EH+CHKT AYHYEP+D +DW+T 
Sbjct: 188  ERFDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTN 247

Query: 893  YVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFET 1072
            Y+FPAGT+ + SAS  LYFQDDVSVVN WTLSGKH+SR++EEWLK +D N+   K +  +
Sbjct: 248  YIFPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVEFKEIMRS 307

Query: 1073 LMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 1204
            +   E+EA+KL+N+WR FC+ G E+FGY NGEEWM +H+LFKKK
Sbjct: 308  ITKTEKEAIKLLNFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK 351


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
            gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
            [Thalictrum flavum]
          Length = 356

 Score =  459 bits (1180), Expect = e-126
 Identities = 209/356 (58%), Positives = 284/356 (79%)
 Frame = +2

Query: 137  VEAKQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTNEEQIAEIQNLTH 316
            +E KQTKK A+  L+K++E G V  ++I+ +++ ++ +RL+WGYKPT+E+Q+A++     
Sbjct: 1    METKQTKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQ 60

Query: 317  SLRQMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYC 496
            SL+ M++A EV+TLD++LYEIP+ FL IM G  LK S  YFK++STTLDE+E+ M+DLYC
Sbjct: 61   SLKGMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYC 120

Query: 497  ERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVE 676
            ERAQI+DGQS+LDLGCG G+LTLHVAQKY+ C+VT +TNSVSQKE+I ++ ++ +L NVE
Sbjct: 121  ERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVE 180

Query: 677  VKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYH 856
            + L D+T  E   TYDRI  + L EHMKNYEL L+K+S WI++ GLLF+EH CHK FAY 
Sbjct: 181  IILEDVTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQ 240

Query: 857  YEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLD 1036
            YEPLD+DDW+TEY+FP+GT+++ S+S  LYFQ+DVSVVNHWTLSGKH S   ++WLKRLD
Sbjct: 241  YEPLDEDDWYTEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLD 300

Query: 1037 ANLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 1204
             N+D +K +FE+  G++E+A+K I YWR FC++  +M+  NNGEEWM S VLFKKK
Sbjct: 301  DNIDEVKEIFESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQVLFKKK 356