BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00014521
         (1454 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum]   370   e-100
gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum]             370   e-100
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   298   2e-78
gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Pap...   295   3e-77
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...   293   6e-77

>gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum]
          Length = 356

 Score =  370 bits (950), Expect = e-100
 Identities = 183/345 (53%), Positives = 253/345 (73%), Gaps = 4/345 (1%)
 Frame = -2

Query: 1351 AQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIA----LSSLATSPTLVSIKPNNL 1184
            +QI N +CG VD+ VL+CA++L +FD I N+   KP+     LSS+ +SP+  SI+P NL
Sbjct: 16   SQIWNQICGTVDTSVLRCAIQLGIFDAIHNS--GKPMITLTELSSIVSSPSSSSIEPCNL 73

Query: 1183 YRLLRYLVHMDLLTIHVVEGNDETFSLTELSKLLLRDQKRSLVDWALAIIDEKVIDGWHE 1004
            YRL+RYL  MDL++I     N+ T SLT  SKLLLR+Q++SL+DW LAI  E ++  WHE
Sbjct: 74   YRLVRYLSQMDLISIGECL-NEATVSLTGTSKLLLRNQEKSLIDWVLAIXCEMMVVVWHE 132

Query: 1003 LSDCCTSPAGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKLL 824
            LS   ++PA  P  F++VHGK+  ELAGE    N +IN+AM SDT +  P  +Q C K+L
Sbjct: 133  LSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVTKPALIQGCGKIL 192

Query: 823  NGITSMVDIGGGVGMAMSYIVKAFPHLKCTVFDLPHVIAASTQLPGVEMVGGDMFKFIPP 644
            NG+TS++D+GGG G  M+YIV+AFPH+K  V DLPHV+ A+ + PGVE + GD+FK I  
Sbjct: 193  NGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERPGVEFISGDIFKSISN 252

Query: 643  ADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAKMNLD 464
            ADA+ LK++LHNW D EC+ +LK+CKE +P DKGKVII+D+V D ++ +  LT+AK++LD
Sbjct: 253  ADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVID-DDDNSILTQAKLSLD 311

Query: 463  IDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 329
            + +M   GGRERT+ +W  L++++GFSRHEIIPI A+ S+IVAYP
Sbjct: 312  LTVMNHGGGRERTKEDWRNLIEMSGFSRHEIIPISAMPSIIVAYP 356


>gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum]
          Length = 356

 Score =  370 bits (949), Expect = e-100
 Identities = 183/345 (53%), Positives = 253/345 (73%), Gaps = 4/345 (1%)
 Frame = -2

Query: 1351 AQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIA----LSSLATSPTLVSIKPNNL 1184
            +QI N +CG VD+ VL+CA++L +FD I N+   KP+     LSS+ +SP+  SI+P NL
Sbjct: 16   SQIWNQICGTVDTSVLRCAIQLGIFDAIHNS--GKPMITLTELSSIVSSPSSSSIEPCNL 73

Query: 1183 YRLLRYLVHMDLLTIHVVEGNDETFSLTELSKLLLRDQKRSLVDWALAIIDEKVIDGWHE 1004
            YRL+RYL  MDL++I     N+ T SLT  SKLLLR+Q++SL+DW LAI  E ++  WHE
Sbjct: 74   YRLVRYLSQMDLISIGECL-NEATVSLTGTSKLLLRNQEKSLIDWVLAISCEMMVVVWHE 132

Query: 1003 LSDCCTSPAGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKLL 824
            LS   ++PA  P  F++VHGK+  ELAGE    N +IN+AM SDT +  P  +Q C K+L
Sbjct: 133  LSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVTKPALIQGCGKIL 192

Query: 823  NGITSMVDIGGGVGMAMSYIVKAFPHLKCTVFDLPHVIAASTQLPGVEMVGGDMFKFIPP 644
            NG+TS++D+GGG G  M+YIV+AFPH+K  V DLPHV+ A+ + PGVE + GD+FK I  
Sbjct: 193  NGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERPGVEFISGDIFKSISN 252

Query: 643  ADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAKMNLD 464
            ADA+ LK++LHNW D EC+ +LK+CKE +P DKGKVII+D+V D ++ +  LT+AK++LD
Sbjct: 253  ADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVID-DDDNSILTQAKLSLD 311

Query: 463  IDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 329
            + +M   GGRERT+ +W  L++++GFSRHEIIPI A+ S+IVAYP
Sbjct: 312  LTVMNHGGGRERTKEDWRNLIEMSGFSRHEIIPISAMPSIIVAYP 356


>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase 2 [Papaver somniferum]
          Length = 357

 Score =  298 bits (764), Expect = 2e-78
 Identities = 149/342 (43%), Positives = 222/342 (64%), Gaps = 1/342 (0%)
 Frame = -2

Query: 1351 AQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIALSSLATSPTLVSIKPNNLYRLL 1172
            AQ+ N + G  DS+VL+CAVE+ + D+I NN  +  I L+ LA    + ++  + LYR++
Sbjct: 21   AQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGA--ITLAQLAAKLPITNVSSDYLYRMV 78

Query: 1171 RYLVHMDLLTIHVVEGN-DETFSLTELSKLLLRDQKRSLVDWALAIIDEKVIDGWHELSD 995
            RYLVH++++      G  ++ +SL  +  LLLRD +RS+V   L +  +  +  WH + +
Sbjct: 79   RYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKE 138

Query: 994  CCTSPAGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKLLNGI 815
               +  G  T FE+  G  +W+    N   +Q+ N+ M  +T L+    ++ C     G+
Sbjct: 139  GLGN--GSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQGL 196

Query: 814  TSMVDIGGGVGMAMSYIVKAFPHLKCTVFDLPHVIAASTQLPGVEMVGGDMFKFIPPADA 635
             S+VDIGGG G  +  I +AFPH+KCT++DLPHV+A S  LP +E V GDMFK +P A A
Sbjct: 197  DSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQA 256

Query: 634  IFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAKMNLDIDM 455
            I LK +LH+W D+EC+ ILKKCKE IP++ GKVII+D+  ++ E + +LT+ ++ LDIDM
Sbjct: 257  ILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEE-ESNHELTKTRLILDIDM 315

Query: 454  MVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 329
            +V +GGRERT ++WE LLK AGF  H+I PI A+QSVI A+P
Sbjct: 316  LVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357


>gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
          Length = 346

 Score =  295 bits (754), Expect = 3e-77
 Identities = 155/345 (44%), Positives = 222/345 (64%), Gaps = 2/345 (0%)
 Frame = -2

Query: 1357 SHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIALSSLATS-PTLVSIKPNNLY 1181
            + A+I   + G  +S+VLKCAV+L + + + NN   KP++LS LA+  P    +  + L+
Sbjct: 11   NQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNV--KPMSLSELASKLPVPQPVNEDRLF 68

Query: 1180 RLLRYLVHMDLLTIHVVEGNDETFSLTELSKLLLRDQKRSLVDWALAIIDEKVIDGWHEL 1001
            R++RYLVHM+L  I   +   + +SL   +K LLR  ++S+VD  L I D+  +  WH L
Sbjct: 69   RIMRYLVHMELFKI---DATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWHHL 125

Query: 1000 SDCCTSPAGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKLLN 821
             D  T   G    FE+  GKS+W    EN   NQ+ N AM  DT LV       C  + +
Sbjct: 126  GDGLT---GNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSIFS 182

Query: 820  -GITSMVDIGGGVGMAMSYIVKAFPHLKCTVFDLPHVIAASTQLPGVEMVGGDMFKFIPP 644
             GI ++VD+GGG G A+  I KAFP +KCT++DLPHVIA S ++P +  + GDMFK IP 
Sbjct: 183  DGIKTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSIPS 242

Query: 643  ADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAKMNLD 464
            ADAIF+K +LH+W+D+ECI ILK+CKE +P+D GKVII+D+V D  +      + ++ LD
Sbjct: 243  ADAIFMKCILHDWNDEECIQILKRCKEALPKD-GKVIIVDVVIDM-DSTHPYAKIRLTLD 300

Query: 463  IDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 329
            +DMM+ +GG+ERT+ EW++L   AGF+ H++  I AVQSVI AYP
Sbjct: 301  LDMMLNTGGKERTKEEWKILFDAAGFASHKVTQISAVQSVIEAYP 345


>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase; Short=6-OMT
            gi|9711914|dbj|BAB08004.1|
            S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score =  293 bits (751), Expect = 6e-77
 Identities = 155/343 (45%), Positives = 222/343 (64%)
 Frame = -2

Query: 1357 SHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIALSSLATSPTLVSIKPNNLYR 1178
            S A++ N + G  +S+VLKCAV+L+L ++I N+  S  + LS L++      +  + LYR
Sbjct: 10   SQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTS--MTLSELSSRLPSQPVNEDALYR 67

Query: 1177 LLRYLVHMDLLTIHVVEGNDETFSLTELSKLLLRDQKRSLVDWALAIIDEKVIDGWHELS 998
            ++RYLVHM L T   ++G +  + L   +K L++   + +V   LAI D+  +  WH L 
Sbjct: 68   VMRYLVHMKLFTKASIDG-ELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPWHYLK 126

Query: 997  DCCTSPAGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKLLNG 818
            D  +  +G  T FE+  G ++W    E+   NQ+ N+AM +D+ L+M   V+ C  + NG
Sbjct: 127  DGLSGESG--TAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNIFNG 184

Query: 817  ITSMVDIGGGVGMAMSYIVKAFPHLKCTVFDLPHVIAASTQLPGVEMVGGDMFKFIPPAD 638
            IT++VD+GGG G A+  I  AFPH+KCTV+DLPHVIA S     V  V GDMFKFIP AD
Sbjct: 185  ITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIPKAD 244

Query: 637  AIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAKMNLDID 458
            AI +K +LH+W DKECI ILK+CKE +P   GKVII+DIV +  + +   T+ ++ LD+D
Sbjct: 245  AIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNV-QSEHPYTKMRLTLDLD 303

Query: 457  MMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 329
            MM+ +GG+ERTE EW+ L+  AG+  H+I  I AVQSVI AYP
Sbjct: 304  MMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346