BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00014267
         (1849 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004151862.1| PREDICTED: tRNA-dihydrouridine(16/17) syntha...   593   e-167
ref|XP_002283225.1| PREDICTED: tRNA-dihydrouridine synthase 1-li...   590   e-166
ref|XP_002865017.1| nitrogen regulation family protein [Arabidop...   586   e-165
ref|XP_002533266.1| tRNA-dihydrouridine synthase, putative [Rici...   586   e-165
ref|NP_201523.1| tRNA-dihydrouridine synthase 4 [Arabidopsis tha...   583   e-164

>ref|XP_004151862.1| PREDICTED: tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
            [Cucumis sativus] gi|449484028|ref|XP_004156763.1|
            PREDICTED: tRNA-dihydrouridine(16/17) synthase
            [NAD(P)(+)]-like [Cucumis sativus]
          Length = 426

 Score =  593 bits (1530), Expect = e-167
 Identities = 293/412 (71%), Positives = 336/412 (81%), Gaps = 15/412 (3%)
 Frame = +2

Query: 116  LNFLFTLLPKSFMTTTKIP---PPTSEHEDDECICPD------------TNDLSLLEQPL 250
            L ++F+L P S M ++ I    P  +  + D+ +C              TN  S    PL
Sbjct: 11   LKYIFSL-PNSLMASSLIQTLKPDLAPQDLDDLLCSPSHKQPQHESSSLTNSTSNDSTPL 69

Query: 251  SSKNSPSCTLSKDLRIERAWAHWKKLGHPKFIVAPMVDNSELPFRMLCRKYGAQAAYTPM 430
                SP+  LS++ RIERAWAHW KLG PK IVAPMVDNSELPFR+LCRKYGA+AAYTPM
Sbjct: 70   LG--SPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM 127

Query: 431  LHSRLFLENEKQRNMEFTTCKEDRPLFVQFCANDPDILLEAAKLVEQHCDYVDINLGCPQ 610
            LHSR+F ENEK RNMEFTTC+EDRPLFVQFCANDPD+LLEAA+ VE +CDYVD+NLGCPQ
Sbjct: 128  LHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ 187

Query: 611  RIARRGNYGAFLMDNLPVVRSLVQSLADNLHVPVSCKIRIFPDMQDTIKYARMLEDAGCS 790
            RIARRGNYGAFLMDNLP+V+++V+ LA NL VPVSCKIRIFP++QDTI YARMLEDAGCS
Sbjct: 188  RIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCS 247

Query: 791  LLAVHGRTRDEKDGKKFRADWNAIKDVKAALRIPVLANGNIRHMDDVLSCLEETGVDGVL 970
            LLAVHGRTRDEKDGKKFRA+W+AI  VK A+RIPVLANGNIRHM+DV  CL+ETGV+GVL
Sbjct: 248  LLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVL 307

Query: 971  SAEPLLENPALFAGFRTAEWVDREEEGIEGDLLDQADLLVEYLKLCERYPVPWRMIRCHV 1150
            SAE LLENPALFAGFRTAEW++  EE      LDQADLLV+YLKLCE+YPVPWRMIR HV
Sbjct: 308  SAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHV 367

Query: 1151 HKLLGDWFKIHPEVREDLNKQSKLTFEFLYDIVKRLRELGTRIPLYAKSSHA 1306
            HKLLGDWFKIHP +RED N QSKLTFEFLY++V+RLREL  ++PLY K SHA
Sbjct: 368  HKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHA 419


>ref|XP_002283225.1| PREDICTED: tRNA-dihydrouridine synthase 1-like [Vitis vinifera]
          Length = 424

 Score =  590 bits (1521), Expect = e-166
 Identities = 294/399 (73%), Positives = 328/399 (82%), Gaps = 3/399 (0%)
 Frame = +2

Query: 137  LPKSFMTTTKIPPPTSEHED---DECICPDTNDLSLLEQPLSSKNSPSCTLSKDLRIERA 307
            +  S   T  +  P    +D   D+ IC +  +    E   SS + PS  L+ + RIERA
Sbjct: 25   MASSISETLTLTLPAGSPQDSNLDDHICSEQQEHQ--ECQSSSADWPSRCLTGESRIERA 82

Query: 308  WAHWKKLGHPKFIVAPMVDNSELPFRMLCRKYGAQAAYTPMLHSRLFLENEKQRNMEFTT 487
            WAHWKKLG PK IVAPMVDNSELPFRMLCRKYGAQAAYTPMLHSR+F ENEK R+ EFTT
Sbjct: 83   WAHWKKLGQPKLIVAPMVDNSELPFRMLCRKYGAQAAYTPMLHSRIFSENEKYRSQEFTT 142

Query: 488  CKEDRPLFVQFCANDPDILLEAAKLVEQHCDYVDINLGCPQRIARRGNYGAFLMDNLPVV 667
            CKEDRPLFVQFCANDPD LLEAA+ VE +CDYVDINLGCPQRIARRGNYGAFLMDNLP+V
Sbjct: 143  CKEDRPLFVQFCANDPDTLLEAAQRVEPYCDYVDINLGCPQRIARRGNYGAFLMDNLPLV 202

Query: 668  RSLVQSLADNLHVPVSCKIRIFPDMQDTIKYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 847
            +SLV+ LA NLHVPVSCKIR+FP++QDTI YARMLEDAGCSLLAVHGRTRDEKDGKKFRA
Sbjct: 203  KSLVEKLALNLHVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 262

Query: 848  DWNAIKDVKAALRIPVLANGNIRHMDDVLSCLEETGVDGVLSAEPLLENPALFAGFRTAE 1027
            DWNAIK VK A+ IPVLANG+IRHMDDV  CLEETG DGVLSAE LLENPALFAGF+TAE
Sbjct: 263  DWNAIKAVKNAVSIPVLANGDIRHMDDVQRCLEETGADGVLSAESLLENPALFAGFQTAE 322

Query: 1028 WVDREEEGIEGDLLDQADLLVEYLKLCERYPVPWRMIRCHVHKLLGDWFKIHPEVREDLN 1207
            W    EEG +   LDQA+LLVEYLKLCE+YPVPWRMIR HVHKLLG+WF+I P+VR+DLN
Sbjct: 323  WALGGEEGNKDGELDQANLLVEYLKLCEKYPVPWRMIRSHVHKLLGEWFRIQPQVRDDLN 382

Query: 1208 KQSKLTFEFLYDIVKRLRELGTRIPLYAKSSHAAFSPKG 1324
             QSKLTFEFLY++V +LRELG RIPLY K + A  +  G
Sbjct: 383  SQSKLTFEFLYNMVDQLRELGVRIPLYTKDTPAGRTSNG 421


>ref|XP_002865017.1| nitrogen regulation family protein [Arabidopsis lyrata subsp. lyrata]
            gi|297310852|gb|EFH41276.1| nitrogen regulation family
            protein [Arabidopsis lyrata subsp. lyrata]
          Length = 423

 Score =  586 bits (1511), Expect = e-165
 Identities = 289/416 (69%), Positives = 336/416 (80%), Gaps = 10/416 (2%)
 Frame = +2

Query: 104  KLPSLNFLFTLLPKSFMTTTKI------PPPTSEHEDDECICPDTNDLSL---LEQPLSS 256
            KL   N  F L  KSF++ T+       P P      D+ +C +  D  +   ++   +S
Sbjct: 2    KLNLSNLRFLLTRKSFISQTRAMTQNPDPKPDPSKVIDDLLCSEQRDGQIEETVDAVAAS 61

Query: 257  KNSPSCTLSKDLRIERAWAHWKKLGHPKFIVAPMVDNSELPFRMLCRKYGAQAAYTPMLH 436
              SPS  LS D R+ERAWAHWKKLG PK+IVAPMVDNSELPFR+LC+KYGAQAAYTPMLH
Sbjct: 62   LGSPSRVLSIDTRVERAWAHWKKLGRPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLH 121

Query: 437  SRLFLENEKQRNMEFTTCKEDRPLFVQFCANDPDILLEAAKLVEQHCDYVDINLGCPQRI 616
            SR+F E EK RN EFTTCKEDRPLFVQFCANDPD LLEAAK VE +CDYVDINLGCPQRI
Sbjct: 122  SRIFTETEKYRNQEFTTCKEDRPLFVQFCANDPDTLLEAAKRVEPYCDYVDINLGCPQRI 181

Query: 617  ARRGNYGAFLMDNLPVVRSLVQSLADNLHVPVSCKIRIFPDMQDTIKYARMLEDAGCSLL 796
            ARRGNYGAFLMDNLP+V+SLV+ LA NL+VPVSCKIRIFP++QDT+KYA+MLEDAGCSLL
Sbjct: 182  ARRGNYGAFLMDNLPLVKSLVEKLAQNLNVPVSCKIRIFPNLQDTLKYAKMLEDAGCSLL 241

Query: 797  AVHGRTRDEKDGKKFRADWNAIKDVKAALRIPVLANGNIRHMDDVLSCLEETGVDGVLSA 976
            AVHGRTRDEKDGKK RADW+AIK+VK ALRIPVL NGN+R ++DV +C++ETGV+GVLSA
Sbjct: 242  AVHGRTRDEKDGKKLRADWSAIKEVKNALRIPVLGNGNVRCIEDVDNCIKETGVEGVLSA 301

Query: 977  EPLLENPALFAGFRTAEWV-DREEEGIEGDLLDQADLLVEYLKLCERYPVPWRMIRCHVH 1153
            E LLENPA FAGFRTAEWV + EEEG     LDQ DL+VEYLKLCE++PVPWRMIR HVH
Sbjct: 302  ETLLENPAAFAGFRTAEWVSENEEEGFVDGGLDQGDLVVEYLKLCEKHPVPWRMIRSHVH 361

Query: 1154 KLLGDWFKIHPEVREDLNKQSKLTFEFLYDIVKRLRELGTRIPLYAKSSHAAFSPK 1321
            K+LG+WF+IHP+VRE LN Q+ LTFEFLY +V +LRELG R+PLY K      +P+
Sbjct: 362  KMLGEWFRIHPQVREQLNAQNILTFEFLYGLVDQLRELGGRVPLYKKKEIDTLTPQ 417


>ref|XP_002533266.1| tRNA-dihydrouridine synthase, putative [Ricinus communis]
            gi|223526922|gb|EEF29128.1| tRNA-dihydrouridine synthase,
            putative [Ricinus communis]
          Length = 426

 Score =  586 bits (1510), Expect = e-165
 Identities = 287/412 (69%), Positives = 329/412 (79%), Gaps = 11/412 (2%)
 Frame = +2

Query: 131  TLLPKSFMTTTKIPPPTSEHEDDECICPDTND----LSLLEQPLSSK----NSPSCTLSK 286
            +L P    + T+      +++DDE +C   +      S L   +SS      +PSC L+ 
Sbjct: 15   SLKPLMASSLTRTVAIDQDNDDDEVLCSSAHQEHTSFSTLPDTVSSSCLGSATPSCYLNG 74

Query: 287  DLRIERAWAHWKKLGHPKFIVAPMVDNSELPFRMLCRKYGAQAAYTPMLHSRLFLENEKQ 466
            + R++RAWAHW K+G PK +VAPMVDNSELPFRMLCRKYGA AAYTPMLHSR+F ENEK 
Sbjct: 75   ESRVDRAWAHWNKIGKPKLLVAPMVDNSELPFRMLCRKYGADAAYTPMLHSRIFTENEKY 134

Query: 467  RNMEFTTCKEDRPLFVQFCANDPDILLEAAKLVEQHCDYVDINLGCPQRIARRGNYGAFL 646
            R  EFTTC EDRPLFVQFCANDPDILLEAA+ VE +CDYVDINLGCPQRIARRGNYGAFL
Sbjct: 135  RTQEFTTCNEDRPLFVQFCANDPDILLEAARKVEPYCDYVDINLGCPQRIARRGNYGAFL 194

Query: 647  MDNLPVVRSLVQSLADNLHVPVSCKIRIFPDMQDTIKYARMLEDAGCSLLAVHGRTRDEK 826
            MDNLP+V+SLV+ LA NL+VPVSCKIR+FP + DTI YARMLE+AGCSLLAVHGRTRDEK
Sbjct: 195  MDNLPLVKSLVEKLALNLNVPVSCKIRLFPKLDDTISYARMLEEAGCSLLAVHGRTRDEK 254

Query: 827  DGKKFRADWNAIKDVKAALRIPVLANGNIRHMDDVLSCLEETGVDGVLSAEPLLENPALF 1006
            DGKKFRADW AIK V+ A++IP+LANGN+RHMDDV +CLEETG DGVLSAE LLENPALF
Sbjct: 255  DGKKFRADWKAIKAVRNAVKIPLLANGNVRHMDDVRNCLEETGADGVLSAESLLENPALF 314

Query: 1007 AGFRTAEWVDREEEGIEGDLLDQADLLVEYLKLCERYPVPWRMIRCHVHKLLGDWFKIHP 1186
            AGFRTAEWV  +EE      LDQADLLVEYLKLCE YPVPWRMIR HVHK+LGDWF+IHP
Sbjct: 315  AGFRTAEWVVGDEESTRDGKLDQADLLVEYLKLCEEYPVPWRMIRAHVHKMLGDWFRIHP 374

Query: 1187 EVREDLNKQSKLTFEFLYDIVKRLRELGTRIPLYAK---SSHAAFSPKGFTN 1333
             VRE+LN QS+LTFEFLY++V +LR LG RIPLY K   +S    +  G TN
Sbjct: 375  HVREELNAQSRLTFEFLYNVVDQLRALGVRIPLYVKDLDTSATGVAANGLTN 426


>ref|NP_201523.1| tRNA-dihydrouridine synthase 4 [Arabidopsis thaliana]
            gi|10177609|dbj|BAB10956.1| unnamed protein product
            [Arabidopsis thaliana] gi|15146316|gb|AAK83641.1|
            AT5g67220/K21H1_18 [Arabidopsis thaliana]
            gi|20908082|gb|AAM26724.1| AT5g67220/K21H1_18
            [Arabidopsis thaliana] gi|332010932|gb|AED98315.1|
            tRNA-dihydrouridine synthase 4 [Arabidopsis thaliana]
          Length = 423

 Score =  583 bits (1503), Expect = e-164
 Identities = 287/416 (68%), Positives = 335/416 (80%), Gaps = 10/416 (2%)
 Frame = +2

Query: 104  KLPSLNFLFTLLPKSFMTTTKI------PPPTSEHEDDECICPDTNDLSL---LEQPLSS 256
            KL   N  F    KS ++ T+       P P      D+ +C +  D  +   ++   +S
Sbjct: 2    KLNLSNLRFLRTRKSLISQTRAMTQNPDPKPDPSQVLDDILCSEQRDGQIEETVDTAPAS 61

Query: 257  KNSPSCTLSKDLRIERAWAHWKKLGHPKFIVAPMVDNSELPFRMLCRKYGAQAAYTPMLH 436
              SPS  LS D R+ERAWAHWKKLG PK+IVAPMVDNSELPFR+LC+KYGAQAAYTPMLH
Sbjct: 62   LGSPSRVLSIDTRVERAWAHWKKLGRPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLH 121

Query: 437  SRLFLENEKQRNMEFTTCKEDRPLFVQFCANDPDILLEAAKLVEQHCDYVDINLGCPQRI 616
            SR+F E EK RN EFTTCKEDRPLFVQFCANDPD LLEAAK VE +CDYVDINLGCPQRI
Sbjct: 122  SRIFTETEKYRNQEFTTCKEDRPLFVQFCANDPDTLLEAAKRVEPYCDYVDINLGCPQRI 181

Query: 617  ARRGNYGAFLMDNLPVVRSLVQSLADNLHVPVSCKIRIFPDMQDTIKYARMLEDAGCSLL 796
            ARRGNYGAFLMDNLP+V+SLV+ LA NL+VPVSCKIRIFP+++DT+KYA+MLEDAGCSLL
Sbjct: 182  ARRGNYGAFLMDNLPLVKSLVEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDAGCSLL 241

Query: 797  AVHGRTRDEKDGKKFRADWNAIKDVKAALRIPVLANGNIRHMDDVLSCLEETGVDGVLSA 976
            AVHGRTRDEKDGKKFRADW+AIK+VK A+RIPVLANGN+R ++DV +C++ETGV+GVLSA
Sbjct: 242  AVHGRTRDEKDGKKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGVLSA 301

Query: 977  EPLLENPALFAGFRTAEWV-DREEEGIEGDLLDQADLLVEYLKLCERYPVPWRMIRCHVH 1153
            E LLENPA FAGFRTAEW  D EEEG     LDQ DL+VEYLKLCE++PVPWRMIR HVH
Sbjct: 302  ETLLENPAAFAGFRTAEWAKDNEEEGFVDGGLDQGDLVVEYLKLCEKHPVPWRMIRSHVH 361

Query: 1154 KLLGDWFKIHPEVREDLNKQSKLTFEFLYDIVKRLRELGTRIPLYAKSSHAAFSPK 1321
            K+LG+WF+IHP+VRE LN Q+ LTFEFLY +V +LRELG R+PLY K      +P+
Sbjct: 362  KMLGEWFRIHPQVREQLNAQNILTFEFLYGLVDQLRELGGRVPLYKKKKIDTLTPQ 417