BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00014041
         (2138 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002268387.1| PREDICTED: HBS1-like protein-like [Vitis vin...   701   0.0  
emb|CBI34018.3| unnamed protein product [Vitis vinifera]              677   0.0  
ref|XP_002330811.1| predicted protein [Populus trichocarpa] gi|2...   668   0.0  
ref|XP_002527965.1| Elongation factor 1-alpha, putative [Ricinus...   656   0.0  
ref|XP_004166761.1| PREDICTED: LOW QUALITY PROTEIN: HBS1-like pr...   654   0.0  

>ref|XP_002268387.1| PREDICTED: HBS1-like protein-like [Vitis vinifera]
          Length = 686

 Score =  701 bits (1809), Expect = 0.0
 Identities = 384/648 (59%), Positives = 445/648 (68%), Gaps = 79/648 (12%)
 Frame = +1

Query: 280  ETFKVNGDT-KSGLWQCSICTFDNHESLFACEICGVIRDPMANIHINGKNEAA---FEAS 447
            E  + N +T + G+W+CSICTFDN ES+ AC+ICGV+R P+ NI  N   + A   F+  
Sbjct: 35   EAVETNQETVRRGIWRCSICTFDNDESMSACDICGVLRYPLVNIRNNNDTKTAPFKFDVP 94

Query: 448  S---------------------------------------NKLSSDKELGSSSVQLESVE 510
            S                                       NK SS K    SS  +    
Sbjct: 95   SPDELVSNGMHASKMASKANLTTSVSSEVPSRVIDKHGLVNKQSSAKRSDRSSDLMPKGR 154

Query: 511  QPTIRESISSFTIGAKSENSLCSLVKENLDLGSGR--SKD------------------YL 630
               + +   S +  A +E+S       ++ +  GR  SKD                   L
Sbjct: 155  HENVGDRDFSESGAANTESSAKGSDSSSMLMPKGRNNSKDESNISSIDKNKRQSISGNLL 214

Query: 631  TTGTAALRSQY---------------KPETWILANQEG-VLPQLNLAIVGHVDXXXXXXX 762
            ++ T  ++S++               KPE W++ +QE  VL QLNLAIVGHVD       
Sbjct: 215  SSMTLNVKSEHSKSSSAGKSVSDVHYKPEKWMIPDQENDVLTQLNLAIVGHVDSGKSTLS 274

Query: 763  XXXXXXXXXXXPKEMRKYEKEAKSQGKGSFAYAWALDESAEERERGITMTVAVAYFHSKK 942
                        KEM KYEKEAK QGKGSFAYAWALDES EERERGITMTVAVAYF SKK
Sbjct: 275  GRLLHLLGRISQKEMHKYEKEAKLQGKGSFAYAWALDESTEERERGITMTVAVAYFDSKK 334

Query: 943  YHVVLLDSPGHRDFVPNMIFGANQADAAVLVIDASVGSFEAGMDVNGGQTREHAQLIRSF 1122
            YHVV+LDSPGH+DFVPNMI GA QAD+A+LVIDAS+G+FEAG+D  GGQTREHAQLIRSF
Sbjct: 335  YHVVVLDSPGHKDFVPNMISGATQADSAILVIDASIGAFEAGVDSTGGQTREHAQLIRSF 394

Query: 1123 GVDQVIVAINKMDTVEYSKERFDSIKGQLGTFLRGCGFRDSFMMWVPLSAMENQNLVTAA 1302
            GVDQ+IVA+NKMD VEYSKERFD IK QLGTFLR CGF+DS + W+PLSAMENQNLV AA
Sbjct: 395  GVDQIIVAVNKMDAVEYSKERFDFIKMQLGTFLRSCGFKDSSVSWIPLSAMENQNLVEAA 454

Query: 1303 SDVRLSSWYQGPFLLDAIDSLQPPTRDISKPLIMPICDVIKSRSTGQLATSGKLETGALR 1482
            SD RLSSWYQGP+LLDAIDSLQPPTRD SKPL+MPICDVIK  S+GQ++  GKLE GALR
Sbjct: 455  SDARLSSWYQGPYLLDAIDSLQPPTRDFSKPLLMPICDVIKPSSSGQVSACGKLEAGALR 514

Query: 1483 SGLKVLVMPSGDSATVRSLERDSKACDIAKAGDNVVVYLQGLDVNQVMAGGVLCHPDYPV 1662
            SG KVLVMPSGD ATVRSLERDS+ C IA+AGDNV V LQG+D + VMAGGVLC PD+PV
Sbjct: 515  SGFKVLVMPSGDVATVRSLERDSQTCAIARAGDNVAVCLQGIDGSNVMAGGVLCQPDFPV 574

Query: 1663 AIATHLELKILVLDVAAPILVGSQMEFHIHHIKEAARVVKILSLFDPKTGKVLKKAPRCL 1842
            A+AT LELK+LVLD+  PIL+GSQ+EFH HH KEAA +VKILSL DPKTGKV K APRC+
Sbjct: 575  AVATRLELKVLVLDIKTPILMGSQLEFHTHHSKEAATIVKILSLLDPKTGKVTKTAPRCV 634

Query: 1843 TAKQSALIEVILDEAVCVEEFSNCRVLGRVFLRAYGRTSAVGIVTRII 1986
            TAKQSA++EV L  AVCVEEFSNCR LGR FLRA GRT AVGIVTR+I
Sbjct: 635  TAKQSAVLEVALSGAVCVEEFSNCRALGRAFLRAMGRTLAVGIVTRVI 682


>emb|CBI34018.3| unnamed protein product [Vitis vinifera]
          Length = 760

 Score =  677 bits (1746), Expect = 0.0
 Identities = 357/525 (68%), Positives = 404/525 (76%), Gaps = 2/525 (0%)
 Frame = +1

Query: 418  GKNEAAFEASSNKLSSDKELGSSSVQLESVEQPTIRES-ISSFTIGAKSENSLCSLVKEN 594
            G  + +   ++N  SS K   SSS+ +      +  ES ISS     +   S   L    
Sbjct: 233  GDRDFSESGAANTESSAKGSDSSSMLMPKGRNNSKDESNISSIDKNKRQSISGNLLSSMT 292

Query: 595  LDLGSGRSKDYLTTGTAALRSQYKPETWILANQEG-VLPQLNLAIVGHVDXXXXXXXXXX 771
            L++ S  SK   + G +     YKPE W++ +QE  VL QLNLAIVGHVD          
Sbjct: 293  LNVKSEHSKSS-SAGKSVSDVHYKPEKWMIPDQENDVLTQLNLAIVGHVDSGKSTLSGRL 351

Query: 772  XXXXXXXXPKEMRKYEKEAKSQGKGSFAYAWALDESAEERERGITMTVAVAYFHSKKYHV 951
                     KEM KYEKEAK QGKGSFAYAWALDES EERERGITMTVAVAYF SKKYHV
Sbjct: 352  LHLLGRISQKEMHKYEKEAKLQGKGSFAYAWALDESTEERERGITMTVAVAYFDSKKYHV 411

Query: 952  VLLDSPGHRDFVPNMIFGANQADAAVLVIDASVGSFEAGMDVNGGQTREHAQLIRSFGVD 1131
            V+LDSPGH+DFVPNMI GA QAD+A+LVIDAS+G+FEAG+D  GGQTREHAQLIRSFGVD
Sbjct: 412  VVLDSPGHKDFVPNMISGATQADSAILVIDASIGAFEAGVDSTGGQTREHAQLIRSFGVD 471

Query: 1132 QVIVAINKMDTVEYSKERFDSIKGQLGTFLRGCGFRDSFMMWVPLSAMENQNLVTAASDV 1311
            Q+IVA+NKMD VEYSKERFD IK QLGTFLR CGF+DS + W+PLSAMENQNLV AASD 
Sbjct: 472  QIIVAVNKMDAVEYSKERFDFIKMQLGTFLRSCGFKDSSVSWIPLSAMENQNLVEAASDA 531

Query: 1312 RLSSWYQGPFLLDAIDSLQPPTRDISKPLIMPICDVIKSRSTGQLATSGKLETGALRSGL 1491
            RLSSWYQGP+LLDAIDSLQPPTRD SKPL+MPICDVIK  S+GQ++  GKLE GALRSG 
Sbjct: 532  RLSSWYQGPYLLDAIDSLQPPTRDFSKPLLMPICDVIKPSSSGQVSACGKLEAGALRSGF 591

Query: 1492 KVLVMPSGDSATVRSLERDSKACDIAKAGDNVVVYLQGLDVNQVMAGGVLCHPDYPVAIA 1671
            KVLVMPSGD ATVRSLERDS+ C IA+AGDNV V LQG+D + VMAGGVLC PD+PVA+A
Sbjct: 592  KVLVMPSGDVATVRSLERDSQTCAIARAGDNVAVCLQGIDGSNVMAGGVLCQPDFPVAVA 651

Query: 1672 THLELKILVLDVAAPILVGSQMEFHIHHIKEAARVVKILSLFDPKTGKVLKKAPRCLTAK 1851
            T LELK+LVLD+  PIL+GSQ+EFH HH KEAA +VKILSL DPKTGKV K APRC+TAK
Sbjct: 652  TRLELKVLVLDIKTPILMGSQLEFHTHHSKEAATIVKILSLLDPKTGKVTKTAPRCVTAK 711

Query: 1852 QSALIEVILDEAVCVEEFSNCRVLGRVFLRAYGRTSAVGIVTRII 1986
            QSA++EV L  AVCVEEFSNCR LGR FLRA GRT AVGIVTR+I
Sbjct: 712  QSAVLEVALSGAVCVEEFSNCRALGRAFLRAMGRTLAVGIVTRVI 756



 Score = 62.4 bits (150), Expect = 5e-07
 Identities = 27/53 (50%), Positives = 37/53 (69%), Gaps = 1/53 (1%)
 Frame = +1

Query: 280 ETFKVNGDT-KSGLWQCSICTFDNHESLFACEICGVIRDPMANIHINGKNEAA 435
           E  + N +T + G+W+CSICTFDN ES+ AC+ICGV+R P+ NI  N   + A
Sbjct: 35  EAVETNQETVRRGIWRCSICTFDNDESMSACDICGVLRYPLVNIRNNNDTKTA 87


>ref|XP_002330811.1| predicted protein [Populus trichocarpa] gi|222872613|gb|EEF09744.1|
            predicted protein [Populus trichocarpa]
          Length = 658

 Score =  668 bits (1723), Expect = 0.0
 Identities = 369/634 (58%), Positives = 430/634 (67%), Gaps = 59/634 (9%)
 Frame = +1

Query: 262  DHYGTEETFKVNGDTKSGLWQCSICTFDNHESLFACEICGVIRDPMANIHINGKNEAAFE 441
            D     E  K     K  +W C ICT+DN ES+ AC+ICGVIR  +     + K  A F+
Sbjct: 25   DQVEAPEPKKKTSSDKVRVWSCPICTYDNDESMSACDICGVIRSSVPGKLKDDKGTAPFK 84

Query: 442  AS--------SNKLSSDKELGSSSVQLESV-----EQPTIRESISSFTIGAKSEN----- 567
                      S  L S K +GS  + L S+        +   SIS    G    N     
Sbjct: 85   FDFPSPDDMVSKGLRSSK-IGSKGILLFSILIGHFVSDSSSASISKGRPGVDEGNHNKNG 143

Query: 568  ------------SLCSLVKENLDLGSGRSKDYLTTG-----------------------T 642
                        S  SL+ +  D   G S   +  G                        
Sbjct: 144  VVDTQSRDEISDSTSSLMPKAKDKSVGYSSSSINGGKSLGLTSNLNDMSLSDKSGNSNKA 203

Query: 643  AALR----SQYKPETWILANQ-EGVLPQLNLAIVGHVDXXXXXXXXXXXXXXXXXXPKEM 807
            +A R    +QY+P+ W+L ++ E  L QLNLAIVGHVD                   KEM
Sbjct: 204  SAKRPKSSAQYQPDKWMLPDKSENALTQLNLAIVGHVDSGKSTLSGRLLHLSGRITQKEM 263

Query: 808  RKYEKEAKSQGKGSFAYAWALDESAEERERGITMTVAVAYFHSKKYHVVLLDSPGHRDFV 987
             KYEKEAK QGKGSFAYAWALDES EERERGITMTVAVAYF SKKYHVV++DSPGH+DFV
Sbjct: 264  HKYEKEAKLQGKGSFAYAWALDESPEERERGITMTVAVAYFDSKKYHVVVIDSPGHKDFV 323

Query: 988  PNMIFGANQADAAVLVIDASVGSFEAGMDVNGGQTREHAQLIRSFGVDQVIVAINKMDTV 1167
            PNMI G+ QADAA+LVIDAS+G FEAGMD N GQTREHA+LIRSFGVDQ+IVA+NKMD+V
Sbjct: 324  PNMISGSTQADAAILVIDASIGGFEAGMD-NKGQTREHARLIRSFGVDQIIVAVNKMDSV 382

Query: 1168 EYSKERFDSIKGQLGTFLRGCGFRDSFMMWVPLSAMENQNLVTAASDVRLSSWYQGPFLL 1347
            EYSK+RFD I+ QLGTFL  CGF+DS + W+PLSA+ENQNLV A SDVRLSSWY G +LL
Sbjct: 383  EYSKDRFDLIRTQLGTFLHSCGFKDSLVSWIPLSAVENQNLVAAPSDVRLSSWYHGSYLL 442

Query: 1348 DAIDSLQPPTRDISKPLIMPICDVIKSRSTGQLATSGKLETGALRSGLKVLVMPSGDSAT 1527
            DAIDSLQP  RD SKPL+MPICDV+KS S GQ++  GKLE GALRSGLKVLVMPSGD  T
Sbjct: 443  DAIDSLQPLKRDFSKPLLMPICDVVKSSSQGQVSACGKLEAGALRSGLKVLVMPSGDVGT 502

Query: 1528 VRSLERDSKACDIAKAGDNVVVYLQGLDVNQVMAGGVLCHPDYPVAIATHLELKILVLD- 1704
            VR+LERDS+ C +A+AGDNV V LQG+D + VMAGGVLCHPD+PVA+A H ELK+LVLD 
Sbjct: 503  VRTLERDSQICAVARAGDNVTVSLQGIDGSNVMAGGVLCHPDFPVAVAKHFELKVLVLDF 562

Query: 1705 VAAPILVGSQMEFHIHHIKEAARVVKILSLFDPKTGKVLKKAPRCLTAKQSALIEVILDE 1884
            +  PI++GSQ+EFHIHH KEAARVVKI+S+ DPKTGKV KKAPRCLT+KQSA+IEV LD 
Sbjct: 563  LTIPIVIGSQLEFHIHHAKEAARVVKIISVLDPKTGKVSKKAPRCLTSKQSAIIEVALDG 622

Query: 1885 AVCVEEFSNCRVLGRVFLRAYGRTSAVGIVTRII 1986
             VC EEF+NCR LGR FLR  G+T AVGIVTRII
Sbjct: 623  PVCAEEFTNCRALGRAFLRTLGKTVAVGIVTRII 656


>ref|XP_002527965.1| Elongation factor 1-alpha, putative [Ricinus communis]
            gi|223532591|gb|EEF34377.1| Elongation factor 1-alpha,
            putative [Ricinus communis]
          Length = 670

 Score =  656 bits (1692), Expect = 0.0
 Identities = 356/625 (56%), Positives = 425/625 (68%), Gaps = 69/625 (11%)
 Frame = +1

Query: 319  WQCSICTFDNHESLFACEICGVIRDPMA-------------------------------- 402
            W+CSICT+DN ES+ AC+ICGVIR+P A                                
Sbjct: 47   WRCSICTYDNDESMNACDICGVIRNPTAGNSNNNDKRTVPFKFDVPSPDNLVSSGLHSSK 106

Query: 403  -------NIHINGKNEA-AFEASSNKLSS------------------------DKELGSS 486
                   N ++ GKNEA A ++SS   SS                        D+   +S
Sbjct: 107  RDSRDSGNDNVRGKNEASAIQSSSGSNSSFSLKPKPGVASNFLEDSALSIHSSDEMPENS 166

Query: 487  SVQLESVEQPTIRESISSFTIGAKSENSLCSLVKENLDLGSGRSKDYLTTGTAALRSQ-- 660
            S  +   +   +  S SS  IG +       ++  N+ + S   K    +   A +S+  
Sbjct: 167  SALMPKGKHRNMDNSSSSSMIGGERH-----MLANNISMMSVSDKSEHVSSINAKKSKSI 221

Query: 661  --YKPETWILANQ-EGVLPQLNLAIVGHVDXXXXXXXXXXXXXXXXXXPKEMRKYEKEAK 831
              Y+P+ W+L ++ +  + QLNLAIVGHVD                   KEM KYEKEAK
Sbjct: 222  AHYQPDNWMLLDKADDTMTQLNLAIVGHVDSGKSTLSGRLLHLLGRITQKEMHKYEKEAK 281

Query: 832  SQGKGSFAYAWALDESAEERERGITMTVAVAYFHSKKYHVVLLDSPGHRDFVPNMIFGAN 1011
             QGKGSFAYAWALDES EERERGITMTVAVAYF SKKYHVV+LDSPGH+DFVPNMI GA 
Sbjct: 282  LQGKGSFAYAWALDESPEERERGITMTVAVAYFDSKKYHVVVLDSPGHKDFVPNMISGAT 341

Query: 1012 QADAAVLVIDASVGSFEAGMDVNGGQTREHAQLIRSFGVDQVIVAINKMDTVEYSKERFD 1191
            QADAA+LVIDA  G+FEAGM+   GQTREH QLIRSFGVDQ+IVAINKMD V+YSK+RFD
Sbjct: 342  QADAAILVIDACTGAFEAGMESKKGQTREHVQLIRSFGVDQIIVAINKMDAVQYSKDRFD 401

Query: 1192 SIKGQLGTFLRGCGFRDSFMMWVPLSAMENQNLVTAASDVRLSSWYQGPFLLDAIDSLQP 1371
            SIK QLG FLR CGF+DS + W+PLSAMENQNLV+A SDV LSSWY GP LLDAID+ QP
Sbjct: 402  SIKTQLGMFLRSCGFKDSSISWIPLSAMENQNLVSAPSDVVLSSWYHGPCLLDAIDAFQP 461

Query: 1372 PTRDISKPLIMPICDVIKSRSTGQLATSGKLETGALRSGLKVLVMPSGDSATVRSLERDS 1551
            P+R+ SKPL+MPICDVIKS S GQ++  GKLE GALR G KVLVMPSGD  TVR+LERDS
Sbjct: 462  PSREFSKPLLMPICDVIKSPSMGQVSACGKLEAGALRIGSKVLVMPSGDVGTVRTLERDS 521

Query: 1552 KACDIAKAGDNVVVYLQGLDVNQVMAGGVLCHPDYPVAIATHLELKILVLDVAAPILVGS 1731
            +AC +A+AGDNV V L G+D + V+AGGVLCHPD+PV +A HLELK+LVLD A PIL+GS
Sbjct: 522  QACSVARAGDNVAVSLVGIDGSNVIAGGVLCHPDFPVPVAKHLELKVLVLDFATPILIGS 581

Query: 1732 QMEFHIHHIKEAARVVKILSLFDPKTGKVLKKAPRCLTAKQSALIEVILDEAVCVEEFSN 1911
            Q+EFH++H KEAARVV+I+SL DPKTGK  KKAPRCLT KQ ALIEV L   VC +EFS+
Sbjct: 582  QLEFHLYHTKEAARVVRIISLLDPKTGKETKKAPRCLTPKQHALIEVDLHGPVCAQEFSS 641

Query: 1912 CRVLGRVFLRAYGRTSAVGIVTRII 1986
            C+ LGRV LR  GRT A+G+VT+II
Sbjct: 642  CKALGRVSLRVLGRTIALGVVTKII 666


>ref|XP_004166761.1| PREDICTED: LOW QUALITY PROTEIN: HBS1-like protein-like [Cucumis
            sativus]
          Length = 618

 Score =  654 bits (1688), Expect = 0.0
 Identities = 344/569 (60%), Positives = 421/569 (73%), Gaps = 12/569 (2%)
 Frame = +1

Query: 316  LWQCSICTFDNHESLFACEICGVIRDPMANIHINGKNEAAF----------EASSNKLSS 465
            LW+CSICT+DN +S   C+ICGV+R P+ N + N +++             +  SN L S
Sbjct: 48   LWRCSICTYDNEDSFSVCDICGVLRIPLDN-NRNTQDDRTVPFKFDIPSPDDVVSNGLRS 106

Query: 466  DKE-LGSSSVQLESVEQPTIRESISSFTIGAKSENSLCSLVKENLDLGSGRSKDYLTTGT 642
             K  L  +S    + +  ++ ES +      +S++    L    L++ S  + +Y++   
Sbjct: 107  SKVGLKGTSSSKSAGKFDSMDESSNPSVDWERSQSLAGGLNNMVLNVKSAYA-NYISGIG 165

Query: 643  AALRSQYKPETWILANQE-GVLPQLNLAIVGHVDXXXXXXXXXXXXXXXXXXPKEMRKYE 819
                 QYK + W+L ++    L QLNLAIVGHVD                   KEM KYE
Sbjct: 166  KTSNPQYKHDKWMLPDKAVDTLTQLNLAIVGHVDSGKSTLSGRLLHLLGRVSQKEMHKYE 225

Query: 820  KEAKSQGKGSFAYAWALDESAEERERGITMTVAVAYFHSKKYHVVLLDSPGHRDFVPNMI 999
            KEAKS GKGSFAYAWALDESAEERERGITMTV VA F SK+YH+V+LDSPGH+DFVPN+I
Sbjct: 226  KEAKSMGKGSFAYAWALDESAEERERGITMTVGVAXFDSKRYHIVVLDSPGHKDFVPNLI 285

Query: 1000 FGANQADAAVLVIDASVGSFEAGMDVNGGQTREHAQLIRSFGVDQVIVAINKMDTVEYSK 1179
             GA QADAAVLVIDASVG+FEAGMD + GQTREH QLIRSFGVDQ+IVA+NKMD VEYSK
Sbjct: 286  SGATQADAAVLVIDASVGAFEAGMDSSKGQTREHVQLIRSFGVDQIIVAVNKMDVVEYSK 345

Query: 1180 ERFDSIKGQLGTFLRGCGFRDSFMMWVPLSAMENQNLVTAASDVRLSSWYQGPFLLDAID 1359
            +R++ IK QLGTF+R CG++DS + W+PLSAM NQNLVTA SDV   SWY+GP LL+AID
Sbjct: 346  DRYEFIKLQLGTFIRSCGYKDSSLSWIPLSAMANQNLVTAPSDVHFLSWYRGPNLLEAID 405

Query: 1360 SLQPPTRDISKPLIMPICDVIKSRSTGQLATSGKLETGALRSGLKVLVMPSGDSATVRSL 1539
            SLQPPTR+ SKPL+MPICDV++S S GQ++  GKLE GAL+SG KVL+MPSGD ATVR+L
Sbjct: 406  SLQPPTREFSKPLLMPICDVVRSLSLGQVSACGKLEAGALQSGSKVLIMPSGDKATVRTL 465

Query: 1540 ERDSKACDIAKAGDNVVVYLQGLDVNQVMAGGVLCHPDYPVAIATHLELKILVLDVAAPI 1719
            ER+S+AC IA+AGDNV V LQG++ + VM+GGVLCHPD+PVA A HLELKIL L+ A PI
Sbjct: 466  ERNSQACKIARAGDNVTVTLQGVEPSSVMSGGVLCHPDFPVAAAKHLELKILTLEYATPI 525

Query: 1720 LVGSQMEFHIHHIKEAARVVKILSLFDPKTGKVLKKAPRCLTAKQSALIEVILDEAVCVE 1899
            L+GSQ+E HIHH+KEAARV +I+SL D KTGKV KKAPRCL+AKQSA+IEV+L   VCVE
Sbjct: 526  LIGSQLEIHIHHVKEAARVARIVSLLDSKTGKVTKKAPRCLSAKQSAVIEVVLQSPVCVE 585

Query: 1900 EFSNCRVLGRVFLRAYGRTSAVGIVTRII 1986
             FS  R LGRVFLR  GRT AVGIVT++I
Sbjct: 586  AFSTSRALGRVFLRTMGRTIAVGIVTQLI 614