BLASTX nr result
ID: Coptis21_contig00010850
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00010850
(1514 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002279313.1| PREDICTED: 12-oxophytodienoate reductase 3 [... 645 0.0
gb|AAY27752.1| 12-oxophytodienoate reductase [Hevea brasiliensis] 633 e-179
ref|XP_002516287.1| 12-oxophytodienoate reductase opr, putative ... 630 e-178
ref|NP_001233873.1| 12-oxophytodienoate reductase 3 [Solanum lyc... 599 e-169
pdb|2HS6|A Chain A, Crystal Structure Of The E291k Mutant Of 12-... 599 e-169
>ref|XP_002279313.1| PREDICTED: 12-oxophytodienoate reductase 3 [Vitis vinifera]
gi|297738573|emb|CBI27818.3| unnamed protein product
[Vitis vinifera] gi|383216818|gb|AFG73688.1|
12-oxophytodienoate reductase 3 protein [Vitis vinifera]
Length = 398
Score = 645 bits (1664), Expect = 0.0
Identities = 309/399 (77%), Positives = 348/399 (87%)
Frame = -2
Query: 1465 MAAAAAEKKNITLFSPYKMSRFNLSHRVVLAPMTRCRALNGIPGAGMVEYYKQRATKGGF 1286
MA ++AE TLFSPYKM RF LSHRV LAPMTRCRA+NGIP + EYY QR+T GGF
Sbjct: 1 MAESSAEGTT-TLFSPYKMGRFQLSHRVALAPMTRCRAMNGIPQPALAEYYSQRSTNGGF 59
Query: 1285 LITEGTTISPTAAGFPRVPGIYTKEQIEAWKKVVDAVHAEGSVIFCQLWHVGRASHHVLQ 1106
LITEGT ISPTAAGFP VPGIYT EQ+EAWKKVV+AVHA+G++IFCQLWHVGRASH V Q
Sbjct: 60 LITEGTLISPTAAGFPHVPGIYTGEQVEAWKKVVEAVHAKGAIIFCQLWHVGRASHQVYQ 119
Query: 1105 PGGTGAPISSTGKPISGRWKALMPDGTYANYSLPRPLETHEIQDIVDHYRQAALNAIEAG 926
PGGTGAPISST KPI+GRW+ LMPDG++ Y PR L+T EI ++V+HYRQ+ALNAI AG
Sbjct: 120 PGGTGAPISSTSKPIAGRWRILMPDGSHGKYPAPRALKTSEIPEVVEHYRQSALNAIAAG 179
Query: 925 FDGIEIHGAHGYLLDQFMKDGINDRTDEYGGSVANRCKFLIWVTQAVAAAIGPDLVGVRI 746
FDGIEIHGAHGYL+DQF+KDGINDRTDEYGGS++NRCKFL+ V QAVAAAIGPD VGVR+
Sbjct: 180 FDGIEIHGAHGYLIDQFLKDGINDRTDEYGGSISNRCKFLVQVVQAVAAAIGPDRVGVRV 239
Query: 745 SPAIDHLEAMDSDPLNLGLTVIKYLNKLQQDFGMKLAYLHVTQPRYTAYGQTESGRHGTE 566
SPAIDHLEA DS+PLNLGL VI+ LNKLQ D+G KL YLHVTQPRYTAYGQTESGRHG+E
Sbjct: 240 SPAIDHLEATDSNPLNLGLAVIERLNKLQLDWGSKLTYLHVTQPRYTAYGQTESGRHGSE 299
Query: 565 EEESRMMRTWRKAYEGTFVCSGGFTRELGVEALANGDADLVSYGRLFISNPDLVLRFKVN 386
EEE+++MRTWR+AY+GTF+CSGGFTRELG+EA+A GDADLVSYGRLFISNPDLVLR KVN
Sbjct: 300 EEEAQLMRTWRRAYQGTFICSGGFTRELGLEAVALGDADLVSYGRLFISNPDLVLRLKVN 359
Query: 385 APLNRYIRATFYTQDSVVGYTDYPFLGQVDSKQQLTSRL 269
APLNRYIRA FYTQD V GYTDYPFL + + SRL
Sbjct: 360 APLNRYIRAYFYTQDPVKGYTDYPFLNKGSESHEPLSRL 398
>gb|AAY27752.1| 12-oxophytodienoate reductase [Hevea brasiliensis]
Length = 399
Score = 633 bits (1633), Expect = e-179
Identities = 305/400 (76%), Positives = 344/400 (86%), Gaps = 1/400 (0%)
Frame = -2
Query: 1465 MAAAAAEKKNIT-LFSPYKMSRFNLSHRVVLAPMTRCRALNGIPGAGMVEYYKQRATKGG 1289
MA E IT LFSPYKM +F+LSHRVVLAPMTRCRALNGIP A +V+YY QR+T GG
Sbjct: 1 MAETGTEGTGITTLFSPYKMGKFSLSHRVVLAPMTRCRALNGIPNAALVDYYTQRSTPGG 60
Query: 1288 FLITEGTTISPTAAGFPRVPGIYTKEQIEAWKKVVDAVHAEGSVIFCQLWHVGRASHHVL 1109
FLITEGT +SPTA GFP VPGIYT+EQ EAWK+VVDAVHA+GS+IFCQLWHVGRASH V
Sbjct: 61 FLITEGTLVSPTAPGFPHVPGIYTEEQAEAWKRVVDAVHAKGSIIFCQLWHVGRASHQVY 120
Query: 1108 QPGGTGAPISSTGKPISGRWKALMPDGTYANYSLPRPLETHEIQDIVDHYRQAALNAIEA 929
QP G APISSTGK IS RW+ LMPDG+Y Y PRPLET EI ++V +YRQ+ALNAI A
Sbjct: 121 QPNGA-APISSTGKAISNRWRILMPDGSYGKYPTPRPLETPEILEVVKNYRQSALNAIRA 179
Query: 928 GFDGIEIHGAHGYLLDQFMKDGINDRTDEYGGSVANRCKFLIWVTQAVAAAIGPDLVGVR 749
GFDGIE+HGAHGYL+DQF+KDGINDRTDEYGGS+ NRC+FL+ V QAV AAIG D VG R
Sbjct: 180 GFDGIEVHGAHGYLIDQFLKDGINDRTDEYGGSINNRCRFLMQVIQAVVAAIGADRVGFR 239
Query: 748 ISPAIDHLEAMDSDPLNLGLTVIKYLNKLQQDFGMKLAYLHVTQPRYTAYGQTESGRHGT 569
+SPAIDHL+A+DSDPLNLGL VI+ LNKLQ + G KL YLHVTQPRYTAYGQTESGRHGT
Sbjct: 240 MSPAIDHLDAIDSDPLNLGLAVIERLNKLQLNLGSKLTYLHVTQPRYTAYGQTESGRHGT 299
Query: 568 EEEESRMMRTWRKAYEGTFVCSGGFTRELGVEALANGDADLVSYGRLFISNPDLVLRFKV 389
EEEE+R+MRTWR+AY+GTF+CSGGFTRELG+EA+A DADLVSYGRLFISNPDLVLRFK+
Sbjct: 300 EEEEARLMRTWRRAYKGTFICSGGFTRELGMEAIAQDDADLVSYGRLFISNPDLVLRFKL 359
Query: 388 NAPLNRYIRATFYTQDSVVGYTDYPFLGQVDSKQQLTSRL 269
NAPLN+Y+R TFYTQD VVGYTDYPF +VD Q+ SRL
Sbjct: 360 NAPLNKYVRKTFYTQDPVVGYTDYPFFRKVDGSQEPRSRL 399
>ref|XP_002516287.1| 12-oxophytodienoate reductase opr, putative [Ricinus communis]
gi|223544773|gb|EEF46289.1| 12-oxophytodienoate reductase
opr, putative [Ricinus communis]
Length = 391
Score = 630 bits (1626), Expect = e-178
Identities = 300/388 (77%), Positives = 337/388 (86%)
Frame = -2
Query: 1432 TLFSPYKMSRFNLSHRVVLAPMTRCRALNGIPGAGMVEYYKQRATKGGFLITEGTTISPT 1253
TLFSPYKM RFNLSHRVVLAPMTRCRALNGIP A + EYY QR+T GGFLITEGT +SPT
Sbjct: 5 TLFSPYKMGRFNLSHRVVLAPMTRCRALNGIPNAALAEYYTQRSTPGGFLITEGTLVSPT 64
Query: 1252 AAGFPRVPGIYTKEQIEAWKKVVDAVHAEGSVIFCQLWHVGRASHHVLQPGGTGAPISST 1073
A GFP VPGIYT+EQ+EAWKKVVD VHA+GS+IFCQLWHVGRASH V QPGG APISST
Sbjct: 65 APGFPHVPGIYTEEQVEAWKKVVDEVHAKGSIIFCQLWHVGRASHQVYQPGGA-APISST 123
Query: 1072 GKPISGRWKALMPDGTYANYSLPRPLETHEIQDIVDHYRQAALNAIEAGFDGIEIHGAHG 893
K IS RW+ LMPDG+Y Y PR LET +IQ++V+ YRQAALNAI AGFDGIEIHGAHG
Sbjct: 124 SKAISNRWRILMPDGSYGTYPTPRALETSDIQEVVETYRQAALNAIRAGFDGIEIHGAHG 183
Query: 892 YLLDQFMKDGINDRTDEYGGSVANRCKFLIWVTQAVAAAIGPDLVGVRISPAIDHLEAMD 713
YL+DQF+KDGINDRTDEYGGS+ NRC+FL+ V +AV AAIG D VG R+SPAIDHL+AMD
Sbjct: 184 YLIDQFLKDGINDRTDEYGGSINNRCRFLMQVIEAVVAAIGADRVGFRMSPAIDHLDAMD 243
Query: 712 SDPLNLGLTVIKYLNKLQQDFGMKLAYLHVTQPRYTAYGQTESGRHGTEEEESRMMRTWR 533
SDPLNLGL VI+ LN+LQ + G KL YLHVTQPRYTAYGQTESGRHGTE+EE+R+MRTWR
Sbjct: 244 SDPLNLGLAVIERLNRLQLNLGSKLTYLHVTQPRYTAYGQTESGRHGTEDEEARLMRTWR 303
Query: 532 KAYEGTFVCSGGFTRELGVEALANGDADLVSYGRLFISNPDLVLRFKVNAPLNRYIRATF 353
Y+GTF+CSGGFTRELG+EA+A G+ADLVSYGR+FISNPDLVLR K+NAPLN+YIR TF
Sbjct: 304 NGYQGTFICSGGFTRELGIEAIAEGNADLVSYGRVFISNPDLVLRLKLNAPLNKYIRKTF 363
Query: 352 YTQDSVVGYTDYPFLGQVDSKQQLTSRL 269
YT D VVGYTDYPFL + + QQL SRL
Sbjct: 364 YTPDPVVGYTDYPFLSKENGSQQLLSRL 391
>ref|NP_001233873.1| 12-oxophytodienoate reductase 3 [Solanum lycopersicum]
gi|62900706|sp|Q9FEW9.1|OPR3_SOLLC RecName:
Full=12-oxophytodienoate reductase 3; AltName:
Full=12-oxophytodienoate-10,11-reductase 3;
Short=OPDA-reductase 3; AltName: Full=LeOPR3
gi|12056507|emb|CAC21424.1| 12-oxophytodienoate reductase
3 [Solanum lycopersicum]
Length = 396
Score = 599 bits (1545), Expect = e-169
Identities = 287/384 (74%), Positives = 331/384 (86%)
Frame = -2
Query: 1459 AAAAEKKNITLFSPYKMSRFNLSHRVVLAPMTRCRALNGIPGAGMVEYYKQRATKGGFLI 1280
A++A+ N LFSPYKM +FNLSHRVVLAPMTRCRALN IP A + EYY+QRAT GGFLI
Sbjct: 2 ASSAQDGNNPLFSPYKMGKFNLSHRVVLAPMTRCRALNNIPQAALGEYYEQRATAGGFLI 61
Query: 1279 TEGTTISPTAAGFPRVPGIYTKEQIEAWKKVVDAVHAEGSVIFCQLWHVGRASHHVLQPG 1100
TEGT ISPT+AGFP VPGI+TKEQ+ WKK+VD VHA+G+VIFCQLWHVGRASH V QP
Sbjct: 62 TEGTMISPTSAGFPHVPGIFTKEQVREWKKIVDVVHAKGAVIFCQLWHVGRASHEVYQPA 121
Query: 1099 GTGAPISSTGKPISGRWKALMPDGTYANYSLPRPLETHEIQDIVDHYRQAALNAIEAGFD 920
G APISST KPIS RW+ LMPDGT+ Y PR + T+EI +V+ YR++ALNAIEAGFD
Sbjct: 122 GA-APISSTEKPISNRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIEAGFD 180
Query: 919 GIEIHGAHGYLLDQFMKDGINDRTDEYGGSVANRCKFLIWVTQAVAAAIGPDLVGVRISP 740
GIEIHGAHGYL+DQF+KDGINDRTDEYGGS+ANRCKF+ V QAV +AIG D VGVR+SP
Sbjct: 181 GIEIHGAHGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGVRVSP 240
Query: 739 AIDHLEAMDSDPLNLGLTVIKYLNKLQQDFGMKLAYLHVTQPRYTAYGQTESGRHGTEEE 560
AIDHL+AMDS+PL+LGL V++ LNK+Q G KLAYLHVTQPRY AYGQTE+GR G+EEE
Sbjct: 241 AIDHLDAMDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLGSEEE 300
Query: 559 ESRMMRTWRKAYEGTFVCSGGFTRELGVEALANGDADLVSYGRLFISNPDLVLRFKVNAP 380
E+R+MRT R AY+GTF+CSGG+TRELG+EA+A GDADLVSYGRLFISNPDLV+R K+NAP
Sbjct: 301 EARLMRTLRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAP 360
Query: 379 LNRYIRATFYTQDSVVGYTDYPFL 308
LN+Y R TFYTQD VVGYTDYPFL
Sbjct: 361 LNKYNRKTFYTQDPVVGYTDYPFL 384
>pdb|2HS6|A Chain A, Crystal Structure Of The E291k Mutant Of 12-
Oxophytodienoate Reductase 3 (Opr3) From Tomato
gi|116667815|pdb|2HS6|B Chain B, Crystal Structure Of The
E291k Mutant Of 12- Oxophytodienoate Reductase 3 (Opr3)
From Tomato gi|116667818|pdb|2HSA|B Chain B, Crystal
Structure Of 12-Oxophytodienoate Reductase 3 (Opr3) From
Tomato gi|116667819|pdb|2HSA|A Chain A, Crystal Structure
Of 12-Oxophytodienoate Reductase 3 (Opr3) From Tomato
gi|256599757|pdb|3HGS|A Chain A, Crystal Structure Of
Tomato Opr3 In Complex With Phb gi|256599758|pdb|3HGS|B
Chain B, Crystal Structure Of Tomato Opr3 In Complex With
Phb
Length = 402
Score = 599 bits (1545), Expect = e-169
Identities = 287/384 (74%), Positives = 331/384 (86%)
Frame = -2
Query: 1459 AAAAEKKNITLFSPYKMSRFNLSHRVVLAPMTRCRALNGIPGAGMVEYYKQRATKGGFLI 1280
A++A+ N LFSPYKM +FNLSHRVVLAPMTRCRALN IP A + EYY+QRAT GGFLI
Sbjct: 8 ASSAQDGNNPLFSPYKMGKFNLSHRVVLAPMTRCRALNNIPQAALGEYYEQRATAGGFLI 67
Query: 1279 TEGTTISPTAAGFPRVPGIYTKEQIEAWKKVVDAVHAEGSVIFCQLWHVGRASHHVLQPG 1100
TEGT ISPT+AGFP VPGI+TKEQ+ WKK+VD VHA+G+VIFCQLWHVGRASH V QP
Sbjct: 68 TEGTMISPTSAGFPHVPGIFTKEQVREWKKIVDVVHAKGAVIFCQLWHVGRASHEVYQPA 127
Query: 1099 GTGAPISSTGKPISGRWKALMPDGTYANYSLPRPLETHEIQDIVDHYRQAALNAIEAGFD 920
G APISST KPIS RW+ LMPDGT+ Y PR + T+EI +V+ YR++ALNAIEAGFD
Sbjct: 128 GA-APISSTEKPISNRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIEAGFD 186
Query: 919 GIEIHGAHGYLLDQFMKDGINDRTDEYGGSVANRCKFLIWVTQAVAAAIGPDLVGVRISP 740
GIEIHGAHGYL+DQF+KDGINDRTDEYGGS+ANRCKF+ V QAV +AIG D VGVR+SP
Sbjct: 187 GIEIHGAHGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGVRVSP 246
Query: 739 AIDHLEAMDSDPLNLGLTVIKYLNKLQQDFGMKLAYLHVTQPRYTAYGQTESGRHGTEEE 560
AIDHL+AMDS+PL+LGL V++ LNK+Q G KLAYLHVTQPRY AYGQTE+GR G+EEE
Sbjct: 247 AIDHLDAMDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLGSEEE 306
Query: 559 ESRMMRTWRKAYEGTFVCSGGFTRELGVEALANGDADLVSYGRLFISNPDLVLRFKVNAP 380
E+R+MRT R AY+GTF+CSGG+TRELG+EA+A GDADLVSYGRLFISNPDLV+R K+NAP
Sbjct: 307 EARLMRTLRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAP 366
Query: 379 LNRYIRATFYTQDSVVGYTDYPFL 308
LN+Y R TFYTQD VVGYTDYPFL
Sbjct: 367 LNKYNRKTFYTQDPVVGYTDYPFL 390