BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00009933
         (3457 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002271820.2| PREDICTED: pullulanase 1, chloroplastic-like...  1380   0.0  
emb|CBI31395.3| unnamed protein product [Vitis vinifera]             1380   0.0  
ref|XP_002532780.1| pullulanase, putative [Ricinus communis] gi|...  1343   0.0  
ref|XP_002315334.1| predicted protein [Populus trichocarpa] gi|2...  1336   0.0  
ref|XP_003536283.1| PREDICTED: pullulanase 1, chloroplastic-like...  1321   0.0  

>ref|XP_002271820.2| PREDICTED: pullulanase 1, chloroplastic-like [Vitis vinifera]
          Length = 896

 Score = 1380 bits (3573), Expect = 0.0
 Identities = 661/892 (74%), Positives = 755/892 (84%)
 Frame = +2

Query: 236  EPEQQLVQFEGTLFYSKAYWVTKSLIAWNVDVGNNASCFLFSSQTADLLVSSNCIQGYDL 415
            E      Q + +L YS+AYWV++S+IAWNVDVG+  SC+L+SS+ A L +  + I G+D+
Sbjct: 4    EVSTSTAQLQDSLLYSRAYWVSESIIAWNVDVGDG-SCYLYSSKIAALSIGDSGITGHDM 62

Query: 416  EIKLEPVKDQLPASVVEKFPHIRDYRVFHVPDDVDVKSLLKCQLAVGLFNANGECSDVTC 595
             I+LE     LP +V+EKFP ++ Y+ F VP  VD KSL+KCQLAV  F+ +G+C++VT 
Sbjct: 63   TIQLEEDNGGLPINVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLAVAAFSCDGQCNNVTG 122

Query: 596  LQLPGILDDLYSYEGPLGAVFSTDSVSLYLWAPTAQAVSACIYGAPSGGDPLETVQLEEV 775
            LQLPG+LD+L+SYEGPLGA+FS ++V+LYLWAPTAQ V ACIYG PSGGDPLE V LEE 
Sbjct: 123  LQLPGVLDELFSYEGPLGAIFSKEAVTLYLWAPTAQVVHACIYGDPSGGDPLEIVCLEEF 182

Query: 776  NGVWSFVGPRSWEGCYYQYEVSVYHPSTLKIENCMVTDPYARGLSSDGRRTLLINLNCEN 955
            NGVWS  GP+SWEGCYY+YEVSVYHPSTL+IE C   DPYARGLS+D RRTLL+NL+ ++
Sbjct: 183  NGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTANDPYARGLSADARRTLLVNLDSDD 242

Query: 956  LKPERWDDLAYEKPELSSFSDISIYELHIRDFSANDQTVNPDLRGGYLAFTCQDSAGLLH 1135
            LKPE WD+LA EKP L SFSDISIYELHIRDFSA+D TV+PD RGGYLAFT QDSAG+LH
Sbjct: 243  LKPEGWDNLADEKPALLSFSDISIYELHIRDFSASDHTVHPDFRGGYLAFTSQDSAGILH 302

Query: 1136 LKKLSNAGLTHVHLLPTFQFAGVDDEKQKWKNLDADRLARLPPDSDEQQAQVTAIQDDDG 1315
            LKKL NAG++H+HLLPTFQFAGVDDEK KWK +D + L  LPPDS EQQAQ+  IQD+DG
Sbjct: 303  LKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVDPNILETLPPDSVEQQAQIMTIQDEDG 362

Query: 1316 YNWGYNPVLWGVPKXXXXXXXXXXXXXLEFRKMVQSLNRIGLRVVLDVVYNHLHGNGPHD 1495
            YNWGYNPVLWGVPK             LEFRKMVQ+LNRIG RVVLDVVYNHLHG+GP D
Sbjct: 363  YNWGYNPVLWGVPKGSYASNPNSPCRTLEFRKMVQALNRIGFRVVLDVVYNHLHGSGPFD 422

Query: 1496 DNSVLDKIVPGYYLRRSTDGSIEHSTCVNNTASEHFMVERLILDDLLCWVVEYKVDGFRF 1675
            +NSVLDKIVPGYYLRR++DG IEHS CVNNTASEHFMVERLILDDLLCW V+YKVDGFRF
Sbjct: 423  ENSVLDKIVPGYYLRRNSDGCIEHSACVNNTASEHFMVERLILDDLLCWAVDYKVDGFRF 482

Query: 1676 DLMGHIMKRTMVKAKNALQGLSKEIHGVDGSMIYIYGEGWDFGEVANNGRGINASQSNLH 1855
            DLMGHIMKRTMVKAKN L  LSK+  G+DGS IYIYGEGWDFGEVA NGRG+NASQ NL 
Sbjct: 483  DLMGHIMKRTMVKAKNMLHSLSKDTDGIDGSSIYIYGEGWDFGEVAKNGRGVNASQFNLC 542

Query: 1856 GTGIGSFNDRIRDAILGGSPFGHPLQQGFLTGLAYQPNGHEHGAKTVVDNMLAESQDHIQ 2035
            GTGIGSFNDRIRDA+LGGSPFGHPLQQGF+TGL  QPN H+HG + V + MLA S+DHIQ
Sbjct: 543  GTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQPNDHDHGGEEVAERMLAVSKDHIQ 602

Query: 2036 VGMAGNLRDFVLTNFQGEEVKGLEVLTYGGSPVAYSSCPMETINYVSAHDNETLFDIISL 2215
            VGMA NL+DFVLTN +GEEVKG EV TY G+PVAY+ CP ETINYVSAHDNETLFDI+SL
Sbjct: 603  VGMAANLKDFVLTNCEGEEVKGSEVSTYDGTPVAYTVCPTETINYVSAHDNETLFDIVSL 662

Query: 2216 KTQMDISVDDRCRLNQLATSIVSLSQGIPFFHCGDEMLRSKSLDRDSYNSGDWFNRLDFS 2395
            KT M ISV++RCR+N LATSI++LSQGIPFFH GDEMLRSKS+DRDSYNSGDWFNRLDFS
Sbjct: 663  KTPMQISVEERCRINHLATSIIALSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFS 722

Query: 2396 YNSNNWGVGLPPKGKNEKNWPLIKSRLADPSFKPHKDHILGAVENFLSVLRIRYSSPLFR 2575
            YNSNNWGVGLPPK KNEKNWPLIK RLADPSFKP K+HI+ AVENFL++L+IRYSSPLFR
Sbjct: 723  YNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPQKNHIIAAVENFLNLLKIRYSSPLFR 782

Query: 2576 LRTSNAIQKRVRFHNVGPSRIPGIIVMSIEDGHEGMPGLAQLDPLYSYIVVAFNPLQNEV 2755
            LRT+N+IQ RVRFHN GPS +PGIIVMSIEDGHEG+PGL+QLDP+YSYIVV  NP   E+
Sbjct: 783  LRTANSIQARVRFHNTGPSWVPGIIVMSIEDGHEGVPGLSQLDPVYSYIVVIINPSPTEI 842

Query: 2756 LFTSHALQARNLVLHPVQLMSSDKLVMESNYDASSGSFTVPPRTTSVFVEPR 2911
             F S ALQAR L LHPVQ+MSSD ++  S Y+ASSG F VPPRTTSVFVEPR
Sbjct: 843  KFASPALQARTLQLHPVQVMSSDGVIKNSTYEASSGCFIVPPRTTSVFVEPR 894


>emb|CBI31395.3| unnamed protein product [Vitis vinifera]
          Length = 956

 Score = 1380 bits (3573), Expect = 0.0
 Identities = 661/892 (74%), Positives = 755/892 (84%)
 Frame = +2

Query: 236  EPEQQLVQFEGTLFYSKAYWVTKSLIAWNVDVGNNASCFLFSSQTADLLVSSNCIQGYDL 415
            E      Q + +L YS+AYWV++S+IAWNVDVG+  SC+L+SS+ A L +  + I G+D+
Sbjct: 64   EVSTSTAQLQDSLLYSRAYWVSESIIAWNVDVGDG-SCYLYSSKIAALSIGDSGITGHDM 122

Query: 416  EIKLEPVKDQLPASVVEKFPHIRDYRVFHVPDDVDVKSLLKCQLAVGLFNANGECSDVTC 595
             I+LE     LP +V+EKFP ++ Y+ F VP  VD KSL+KCQLAV  F+ +G+C++VT 
Sbjct: 123  TIQLEEDNGGLPINVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLAVAAFSCDGQCNNVTG 182

Query: 596  LQLPGILDDLYSYEGPLGAVFSTDSVSLYLWAPTAQAVSACIYGAPSGGDPLETVQLEEV 775
            LQLPG+LD+L+SYEGPLGA+FS ++V+LYLWAPTAQ V ACIYG PSGGDPLE V LEE 
Sbjct: 183  LQLPGVLDELFSYEGPLGAIFSKEAVTLYLWAPTAQVVHACIYGDPSGGDPLEIVCLEEF 242

Query: 776  NGVWSFVGPRSWEGCYYQYEVSVYHPSTLKIENCMVTDPYARGLSSDGRRTLLINLNCEN 955
            NGVWS  GP+SWEGCYY+YEVSVYHPSTL+IE C   DPYARGLS+D RRTLL+NL+ ++
Sbjct: 243  NGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTANDPYARGLSADARRTLLVNLDSDD 302

Query: 956  LKPERWDDLAYEKPELSSFSDISIYELHIRDFSANDQTVNPDLRGGYLAFTCQDSAGLLH 1135
            LKPE WD+LA EKP L SFSDISIYELHIRDFSA+D TV+PD RGGYLAFT QDSAG+LH
Sbjct: 303  LKPEGWDNLADEKPALLSFSDISIYELHIRDFSASDHTVHPDFRGGYLAFTSQDSAGILH 362

Query: 1136 LKKLSNAGLTHVHLLPTFQFAGVDDEKQKWKNLDADRLARLPPDSDEQQAQVTAIQDDDG 1315
            LKKL NAG++H+HLLPTFQFAGVDDEK KWK +D + L  LPPDS EQQAQ+  IQD+DG
Sbjct: 363  LKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVDPNILETLPPDSVEQQAQIMTIQDEDG 422

Query: 1316 YNWGYNPVLWGVPKXXXXXXXXXXXXXLEFRKMVQSLNRIGLRVVLDVVYNHLHGNGPHD 1495
            YNWGYNPVLWGVPK             LEFRKMVQ+LNRIG RVVLDVVYNHLHG+GP D
Sbjct: 423  YNWGYNPVLWGVPKGSYASNPNSPCRTLEFRKMVQALNRIGFRVVLDVVYNHLHGSGPFD 482

Query: 1496 DNSVLDKIVPGYYLRRSTDGSIEHSTCVNNTASEHFMVERLILDDLLCWVVEYKVDGFRF 1675
            +NSVLDKIVPGYYLRR++DG IEHS CVNNTASEHFMVERLILDDLLCW V+YKVDGFRF
Sbjct: 483  ENSVLDKIVPGYYLRRNSDGCIEHSACVNNTASEHFMVERLILDDLLCWAVDYKVDGFRF 542

Query: 1676 DLMGHIMKRTMVKAKNALQGLSKEIHGVDGSMIYIYGEGWDFGEVANNGRGINASQSNLH 1855
            DLMGHIMKRTMVKAKN L  LSK+  G+DGS IYIYGEGWDFGEVA NGRG+NASQ NL 
Sbjct: 543  DLMGHIMKRTMVKAKNMLHSLSKDTDGIDGSSIYIYGEGWDFGEVAKNGRGVNASQFNLC 602

Query: 1856 GTGIGSFNDRIRDAILGGSPFGHPLQQGFLTGLAYQPNGHEHGAKTVVDNMLAESQDHIQ 2035
            GTGIGSFNDRIRDA+LGGSPFGHPLQQGF+TGL  QPN H+HG + V + MLA S+DHIQ
Sbjct: 603  GTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQPNDHDHGGEEVAERMLAVSKDHIQ 662

Query: 2036 VGMAGNLRDFVLTNFQGEEVKGLEVLTYGGSPVAYSSCPMETINYVSAHDNETLFDIISL 2215
            VGMA NL+DFVLTN +GEEVKG EV TY G+PVAY+ CP ETINYVSAHDNETLFDI+SL
Sbjct: 663  VGMAANLKDFVLTNCEGEEVKGSEVSTYDGTPVAYTVCPTETINYVSAHDNETLFDIVSL 722

Query: 2216 KTQMDISVDDRCRLNQLATSIVSLSQGIPFFHCGDEMLRSKSLDRDSYNSGDWFNRLDFS 2395
            KT M ISV++RCR+N LATSI++LSQGIPFFH GDEMLRSKS+DRDSYNSGDWFNRLDFS
Sbjct: 723  KTPMQISVEERCRINHLATSIIALSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFS 782

Query: 2396 YNSNNWGVGLPPKGKNEKNWPLIKSRLADPSFKPHKDHILGAVENFLSVLRIRYSSPLFR 2575
            YNSNNWGVGLPPK KNEKNWPLIK RLADPSFKP K+HI+ AVENFL++L+IRYSSPLFR
Sbjct: 783  YNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPQKNHIIAAVENFLNLLKIRYSSPLFR 842

Query: 2576 LRTSNAIQKRVRFHNVGPSRIPGIIVMSIEDGHEGMPGLAQLDPLYSYIVVAFNPLQNEV 2755
            LRT+N+IQ RVRFHN GPS +PGIIVMSIEDGHEG+PGL+QLDP+YSYIVV  NP   E+
Sbjct: 843  LRTANSIQARVRFHNTGPSWVPGIIVMSIEDGHEGVPGLSQLDPVYSYIVVIINPSPTEI 902

Query: 2756 LFTSHALQARNLVLHPVQLMSSDKLVMESNYDASSGSFTVPPRTTSVFVEPR 2911
             F S ALQAR L LHPVQ+MSSD ++  S Y+ASSG F VPPRTTSVFVEPR
Sbjct: 903  KFASPALQARTLQLHPVQVMSSDGVIKNSTYEASSGCFIVPPRTTSVFVEPR 954


>ref|XP_002532780.1| pullulanase, putative [Ricinus communis] gi|223527468|gb|EEF29599.1|
            pullulanase, putative [Ricinus communis]
          Length = 964

 Score = 1343 bits (3476), Expect = 0.0
 Identities = 658/923 (71%), Positives = 750/923 (81%), Gaps = 3/923 (0%)
 Frame = +2

Query: 152  SKHSIIPFLSSN-NIKISYNSHCHSST--LAEPEQQLVQFEGTLFYSKAYWVTKSLIAWN 322
            + H  + + SSN      Y   C SS+  L E      QF+ +L YS+A+WV+K++IAWN
Sbjct: 42   NSHRQLLYRSSNPKTSFKYPIRCSSSSMPLQELSTSTSQFQDSLLYSRAFWVSKTIIAWN 101

Query: 323  VDVGNNASCFLFSSQTADLLVSSNCIQGYDLEIKLEPVKDQLPASVVEKFPHIRDYRVFH 502
            VDVG+N SCFL++S T  L VS+  IQG+D+E+KLE     LP +VV KFPHIRDYR F 
Sbjct: 102  VDVGDNGSCFLYASDTGSLSVSNAGIQGHDVEVKLEKYNGGLPENVVVKFPHIRDYRAFK 161

Query: 503  VPDDVDVKSLLKCQLAVGLFNANGECSDVTCLQLPGILDDLYSYEGPLGAVFSTDSVSLY 682
             P  +D KSLLKCQLAV  + A+G+C   T LQLPGILD+LYSY+GPLGA +S ++VSL+
Sbjct: 162  APPTLDAKSLLKCQLAVASYEADGKCRSATGLQLPGILDELYSYDGPLGAHYSKNAVSLH 221

Query: 683  LWAPTAQAVSACIYGAPSGGDPLETVQLEEVNGVWSFVGPRSWEGCYYQYEVSVYHPSTL 862
            LWAPTAQAV   IY       PLE  QL+EVNGVWS  GP+ WEGCYY YEVSVYHPSTL
Sbjct: 222  LWAPTAQAVLVYIYKDSFSKVPLEIHQLKEVNGVWSIKGPKDWEGCYYVYEVSVYHPSTL 281

Query: 863  KIENCMVTDPYARGLSSDGRRTLLINLNCENLKPERWDDLAYEKPELSSFSDISIYELHI 1042
            +IE C   DPYARGLSSDG+RTLL+NL  E+LKPE WD+LA EKP L SF DIS+YELHI
Sbjct: 282  RIEKCYANDPYARGLSSDGKRTLLVNLTSESLKPEGWDNLAKEKPSLLSFCDISLYELHI 341

Query: 1043 RDFSANDQTVNPDLRGGYLAFTCQDSAGLLHLKKLSNAGLTHVHLLPTFQFAGVDDEKQK 1222
            RDFSANDQTV+PD RGGYLAFT ++SAG+LHLKKLSNAG+THVHLLPTFQFAGVDD K+ 
Sbjct: 342  RDFSANDQTVHPDFRGGYLAFTFENSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDVKEN 401

Query: 1223 WKNLDADRLARLPPDSDEQQAQVTAIQDDDGYNWGYNPVLWGVPKXXXXXXXXXXXXXLE 1402
            WK +D   L  L PDS EQQAQ+TA+QD+DGYNWGYNPVLWGVPK             ++
Sbjct: 402  WKCVDNKMLETLLPDSIEQQAQITAVQDNDGYNWGYNPVLWGVPKGSYASNPNGPSRTIQ 461

Query: 1403 FRKMVQSLNRIGLRVVLDVVYNHLHGNGPHDDNSVLDKIVPGYYLRRSTDGSIEHSTCVN 1582
            FRKMVQ+LNRIGLRVVLDVVYNHLHG+GP D+NSVLDKIVPGYYLRR+ DG IE+STCVN
Sbjct: 462  FRKMVQALNRIGLRVVLDVVYNHLHGSGPFDENSVLDKIVPGYYLRRNIDGIIENSTCVN 521

Query: 1583 NTASEHFMVERLILDDLLCWVVEYKVDGFRFDLMGHIMKRTMVKAKNALQGLSKEIHGVD 1762
            NTASEH+MVERLI+DDLL W V YKVDGFRFDLMGH+MK TMVKAK AL  LS E  GVD
Sbjct: 522  NTASEHYMVERLIIDDLLNWAVNYKVDGFRFDLMGHLMKSTMVKAKIALHSLSVERDGVD 581

Query: 1763 GSMIYIYGEGWDFGEVANNGRGINASQSNLHGTGIGSFNDRIRDAILGGSPFGHPLQQGF 1942
            GS +YIYGEGWDFGEVA NGRG+NASQ NL+GTGIGSFNDRIRDA+LGGSPFGHPL QGF
Sbjct: 582  GSSLYIYGEGWDFGEVAKNGRGVNASQFNLYGTGIGSFNDRIRDAMLGGSPFGHPLHQGF 641

Query: 1943 LTGLAYQPNGHEHGAKTVVDNMLAESQDHIQVGMAGNLRDFVLTNFQGEEVKGLEVLTYG 2122
            +TGL  QPNGH+HG K V + ML  ++DHIQVGMA NLRDFVL N +G+EVKG E+ TYG
Sbjct: 642  VTGLMLQPNGHDHGGKDVEELMLTTAKDHIQVGMAANLRDFVLINSEGKEVKGSEITTYG 701

Query: 2123 GSPVAYSSCPMETINYVSAHDNETLFDIISLKTQMDISVDDRCRLNQLATSIVSLSQGIP 2302
            G PVAY+  P ETINYVSAHDNETLFDI+S+KT M+ISVD+RCRLN LATSI++LSQGIP
Sbjct: 702  GEPVAYALSPTETINYVSAHDNETLFDIVSMKTPMEISVDERCRLNHLATSIIALSQGIP 761

Query: 2303 FFHCGDEMLRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKNWPLIKSRLAD 2482
            FFH GDEMLRSKSLDRDSYNSGDWFNRLDFSYNSNNW VGLPP+ KNEKNWPLIK RLAD
Sbjct: 762  FFHAGDEMLRSKSLDRDSYNSGDWFNRLDFSYNSNNWAVGLPPQKKNEKNWPLIKPRLAD 821

Query: 2483 PSFKPHKDHILGAVENFLSVLRIRYSSPLFRLRTSNAIQKRVRFHNVGPSRIPGIIVMSI 2662
            PSFKP K HI+ A ENFL VL++RYSSPLFRL T+NAIQ+RVRFHN GPS IPG+IVMSI
Sbjct: 822  PSFKPQKHHIVAATENFLDVLQMRYSSPLFRLTTANAIQERVRFHNTGPSWIPGVIVMSI 881

Query: 2663 EDGHEGMPGLAQLDPLYSYIVVAFNPLQNEVLFTSHALQARNLVLHPVQLMSSDKLVMES 2842
            EDGHEG PGL+QLDP+YSYIVV FN    +V FTS AL+AR   LHPVQ+ S+D++V  S
Sbjct: 882  EDGHEGFPGLSQLDPIYSYIVVIFNTRPTKVSFTSPALRARTFELHPVQVKSADEVVKNS 941

Query: 2843 NYDASSGSFTVPPRTTSVFVEPR 2911
             Y+ASSG FTVPP TTSVFVE R
Sbjct: 942  RYEASSGCFTVPPITTSVFVEHR 964


>ref|XP_002315334.1| predicted protein [Populus trichocarpa] gi|222864374|gb|EEF01505.1|
            predicted protein [Populus trichocarpa]
          Length = 893

 Score = 1336 bits (3458), Expect = 0.0
 Identities = 631/883 (71%), Positives = 741/883 (83%)
 Frame = +2

Query: 263  EGTLFYSKAYWVTKSLIAWNVDVGNNASCFLFSSQTADLLVSSNCIQGYDLEIKLEPVKD 442
            + +L YS+A+WVT+S+IAWN DV  + SC+L++SQTA L V+   ++G+D +IKLE    
Sbjct: 11   QDSLLYSRAFWVTQSIIAWNADVVRDGSCYLYASQTAALSVTDGEVEGHDFKIKLEEDSG 70

Query: 443  QLPASVVEKFPHIRDYRVFHVPDDVDVKSLLKCQLAVGLFNANGECSDVTCLQLPGILDD 622
             +P +V+ KFPH+RDY+ F VP  VD KSL+KCQLAV  F ++G+CS  T LQLPG+LD+
Sbjct: 71   GIPQNVIAKFPHVRDYKAFKVPSTVDAKSLVKCQLAVATFGSDGKCSYATGLQLPGVLDE 130

Query: 623  LYSYEGPLGAVFSTDSVSLYLWAPTAQAVSACIYGAPSGGDPLETVQLEEVNGVWSFVGP 802
            L++Y+GPLGA +S D+VSLYLWAPTAQAV AC+Y   +  DP+E VQL+EVNGVWS  G 
Sbjct: 131  LFAYDGPLGAHYSEDAVSLYLWAPTAQAVCACVYKNANSRDPVEVVQLKEVNGVWSVEGS 190

Query: 803  RSWEGCYYQYEVSVYHPSTLKIENCMVTDPYARGLSSDGRRTLLINLNCENLKPERWDDL 982
            + WEGCYY YEVSVYHPSTL +E C   DPYARGLS D +RTL +NL+ + LKPE W+ L
Sbjct: 191  KDWEGCYYVYEVSVYHPSTLHVEKCYANDPYARGLSPDSQRTLFVNLDSDTLKPEGWEKL 250

Query: 983  AYEKPELSSFSDISIYELHIRDFSANDQTVNPDLRGGYLAFTCQDSAGLLHLKKLSNAGL 1162
            A EKP + SFSDISIYELH+RDFSAND TV+PD RGGYLAFT +DSAG+LHLKKLSNAG+
Sbjct: 251  ADEKPIILSFSDISIYELHVRDFSANDHTVHPDFRGGYLAFTLEDSAGVLHLKKLSNAGI 310

Query: 1163 THVHLLPTFQFAGVDDEKQKWKNLDADRLARLPPDSDEQQAQVTAIQDDDGYNWGYNPVL 1342
            THVHLLPTFQFAGVDD K+ WK +D+  L +LPPDS EQQAQ+T IQDDDGYNWGYNPVL
Sbjct: 311  THVHLLPTFQFAGVDDVKENWKCVDSTVLEKLPPDSTEQQAQITLIQDDDGYNWGYNPVL 370

Query: 1343 WGVPKXXXXXXXXXXXXXLEFRKMVQSLNRIGLRVVLDVVYNHLHGNGPHDDNSVLDKIV 1522
            WGVPK             +EFRKMVQ+LN IGLRVVLDVVYNHLHGNGP D+NSVLDKIV
Sbjct: 371  WGVPKGSYASNPSGSCRTIEFRKMVQALNHIGLRVVLDVVYNHLHGNGPFDENSVLDKIV 430

Query: 1523 PGYYLRRSTDGSIEHSTCVNNTASEHFMVERLILDDLLCWVVEYKVDGFRFDLMGHIMKR 1702
            PGYYLRR+TDG IEHSTCVNNTASEH+MVER+I+DD+L W   YKVDGFRFDLMGHIMK 
Sbjct: 431  PGYYLRRNTDGFIEHSTCVNNTASEHYMVERMIIDDMLNWAGNYKVDGFRFDLMGHIMKS 490

Query: 1703 TMVKAKNALQGLSKEIHGVDGSMIYIYGEGWDFGEVANNGRGINASQSNLHGTGIGSFND 1882
            TMVKAK+A+  L+KE  G+DGS +YIYGEGWDFGEVANNGRGINASQ N+ GTGIGSFND
Sbjct: 491  TMVKAKDAVNRLTKERDGIDGSSVYIYGEGWDFGEVANNGRGINASQFNVGGTGIGSFND 550

Query: 1883 RIRDAILGGSPFGHPLQQGFLTGLAYQPNGHEHGAKTVVDNMLAESQDHIQVGMAGNLRD 2062
            RIRDA+LGGSPFGHPLQQGF+TGL  QPNGH+HG K V + ML+ ++DHIQVGMAGNLRD
Sbjct: 551  RIRDAMLGGSPFGHPLQQGFVTGLMLQPNGHDHGGKDVEELMLSAAKDHIQVGMAGNLRD 610

Query: 2063 FVLTNFQGEEVKGLEVLTYGGSPVAYSSCPMETINYVSAHDNETLFDIISLKTQMDISVD 2242
            +VLTN  G+EVKG+EVLTYGG+PVAY+  P ETINYVSAHDNETLFD++S+KT M+ISVD
Sbjct: 611  YVLTNSDGKEVKGMEVLTYGGAPVAYALHPAETINYVSAHDNETLFDVVSMKTPMEISVD 670

Query: 2243 DRCRLNQLATSIVSLSQGIPFFHCGDEMLRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVG 2422
            +RCRLN LA+S+++LSQGIPFFH GDEMLRSKSLDRDSYNSGDWFNRLDF+YNSNNWGVG
Sbjct: 671  ERCRLNHLASSVIALSQGIPFFHSGDEMLRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVG 730

Query: 2423 LPPKGKNEKNWPLIKSRLADPSFKPHKDHILGAVENFLSVLRIRYSSPLFRLRTSNAIQK 2602
            LPPK KNEK+WPLI+ RLADPSFKP K+HIL A+ NFL VL+IRYSSPLFRL T+NAIQ+
Sbjct: 731  LPPKQKNEKHWPLIRPRLADPSFKPQKNHILAAINNFLDVLQIRYSSPLFRLTTANAIQE 790

Query: 2603 RVRFHNVGPSRIPGIIVMSIEDGHEGMPGLAQLDPLYSYIVVAFNPLQNEVLFTSHALQA 2782
            RVRFHN GPS +PG+IVMS EDGH G+PGL QLDP+YS+IVV FN   +EV F S  L+A
Sbjct: 791  RVRFHNTGPSWVPGVIVMSFEDGHRGVPGLTQLDPIYSFIVVIFNASPSEVSFASPVLRA 850

Query: 2783 RNLVLHPVQLMSSDKLVMESNYDASSGSFTVPPRTTSVFVEPR 2911
            R   LHP+Q MS+D++V  S+Y+ S+G FTVPPRTTSVFVE R
Sbjct: 851  RTFQLHPIQAMSTDEVVKNSSYETSTGCFTVPPRTTSVFVEYR 893


>ref|XP_003536283.1| PREDICTED: pullulanase 1, chloroplastic-like [Glycine max]
          Length = 951

 Score = 1321 bits (3420), Expect = 0.0
 Identities = 638/897 (71%), Positives = 743/897 (82%)
 Frame = +2

Query: 221  SSTLAEPEQQLVQFEGTLFYSKAYWVTKSLIAWNVDVGNNASCFLFSSQTADLLVSSNCI 400
            SS+  E      Q +  L YS+AYWV +SLIAW+VDVGN  SC+L +S+ A L +++  I
Sbjct: 53   SSSYVEQSASSSQMQNGLLYSRAYWVAESLIAWDVDVGNGFSCYLLASKNASLTIANCQI 112

Query: 401  QGYDLEIKLEPVKDQLPASVVEKFPHIRDYRVFHVPDDVDVKSLLKCQLAVGLFNANGEC 580
            QG DL+IKL+  +  LPA+VVEKFPHIR ++VF +P  +DVK LLK +LAV + +++GEC
Sbjct: 113  QGEDLKIKLQEDRVGLPANVVEKFPHIRGHKVFSLPPTLDVKPLLKFRLAVVICDSDGEC 172

Query: 581  SDVTCLQLPGILDDLYSYEGPLGAVFSTDSVSLYLWAPTAQAVSACIYGAPSGGDPLETV 760
             + T LQLPG+LDDL+SY GPLGA+FS ++VSLYLWAPTAQAV A IY  PSG DP+E V
Sbjct: 173  MNCTGLQLPGVLDDLFSYSGPLGALFSEEAVSLYLWAPTAQAVHAYIYKDPSGDDPIEIV 232

Query: 761  QLEEVNGVWSFVGPRSWEGCYYQYEVSVYHPSTLKIENCMVTDPYARGLSSDGRRTLLIN 940
             LEE NGVW   GP+SWEGCYY YEV VYHPST++IE C  +DPYARGLSSDGRR+ L+N
Sbjct: 233  CLEEENGVWRTKGPKSWEGCYYVYEVCVYHPSTMRIEKCYTSDPYARGLSSDGRRSFLLN 292

Query: 941  LNCENLKPERWDDLAYEKPELSSFSDISIYELHIRDFSANDQTVNPDLRGGYLAFTCQDS 1120
            L+   LKP+ WD+LA +KP + SFSDISIYE+HIRDFSA+D +V P+ RGGYLAFT QDS
Sbjct: 293  LDSVKLKPDGWDNLANKKPTIHSFSDISIYEMHIRDFSASDLSVQPEFRGGYLAFTLQDS 352

Query: 1121 AGLLHLKKLSNAGLTHVHLLPTFQFAGVDDEKQKWKNLDADRLARLPPDSDEQQAQVTAI 1300
            AG+LHLKKLS+AG+THVHLLPTFQFAGVDD+K+ W+ +D   L  LPPDSD+QQA +TAI
Sbjct: 353  AGVLHLKKLSSAGITHVHLLPTFQFAGVDDQKEDWRFVDTSILESLPPDSDQQQALITAI 412

Query: 1301 QDDDGYNWGYNPVLWGVPKXXXXXXXXXXXXXLEFRKMVQSLNRIGLRVVLDVVYNHLHG 1480
            Q+ DGYNWGYNPVLWGVPK             +EFRKMV +LN IGLRVVLDVVYNHL G
Sbjct: 413  QNFDGYNWGYNPVLWGVPKGSYASNPNGPYRTIEFRKMVMALNHIGLRVVLDVVYNHLQG 472

Query: 1481 NGPHDDNSVLDKIVPGYYLRRSTDGSIEHSTCVNNTASEHFMVERLILDDLLCWVVEYKV 1660
            +GP D++SVLDKIVPGYYLRR++DG IEHSTC+NNTASEHFMVERLILDDL+ W V YK+
Sbjct: 473  SGPFDEHSVLDKIVPGYYLRRNSDGLIEHSTCINNTASEHFMVERLILDDLVHWAVNYKI 532

Query: 1661 DGFRFDLMGHIMKRTMVKAKNALQGLSKEIHGVDGSMIYIYGEGWDFGEVANNGRGINAS 1840
            DGFRFDLMGHIMK TMVKAK ALQ L+KE  G+DGS IYIYGEGWDFGEVA NGRG+NAS
Sbjct: 533  DGFRFDLMGHIMKSTMVKAKTALQCLTKEKDGLDGSSIYIYGEGWDFGEVAKNGRGVNAS 592

Query: 1841 QSNLHGTGIGSFNDRIRDAILGGSPFGHPLQQGFLTGLAYQPNGHEHGAKTVVDNMLAES 2020
            Q NL GT IGSFNDRIRDAILGGSPFGHPLQQGF+TGL  QPNGH+HG +    +MLA S
Sbjct: 593  QFNLPGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLLLQPNGHDHGTEANAKSMLAAS 652

Query: 2021 QDHIQVGMAGNLRDFVLTNFQGEEVKGLEVLTYGGSPVAYSSCPMETINYVSAHDNETLF 2200
             DHIQ+GMA NL+DFVLTN +GEEVKG E+LTYGG+PVAY+SCP+ETINYVSAHDNETLF
Sbjct: 653  MDHIQIGMAANLKDFVLTNSEGEEVKGSEILTYGGTPVAYASCPIETINYVSAHDNETLF 712

Query: 2201 DIISLKTQMDISVDDRCRLNQLATSIVSLSQGIPFFHCGDEMLRSKSLDRDSYNSGDWFN 2380
            DI+SLKT MDISV +RCR+N LATSI++LSQGIPFFH GDE+LRSKSLDRDSYNSGDWFN
Sbjct: 713  DIVSLKTPMDISVSERCRINHLATSIIALSQGIPFFHSGDEILRSKSLDRDSYNSGDWFN 772

Query: 2381 RLDFSYNSNNWGVGLPPKGKNEKNWPLIKSRLADPSFKPHKDHILGAVENFLSVLRIRYS 2560
            RLDF+YNSNNWGVGLPP+ KNEKNWPLIK RLA+PSF+P K  IL  V+NFL++LRIRYS
Sbjct: 773  RLDFTYNSNNWGVGLPPQEKNEKNWPLIKPRLANPSFRPQKTDILATVDNFLNLLRIRYS 832

Query: 2561 SPLFRLRTSNAIQKRVRFHNVGPSRIPGIIVMSIEDGHEGMPGLAQLDPLYSYIVVAFNP 2740
            SPLFRL+T+N IQ+RVRFHN GPS + G+IVMSIEDGH G PGL+QLDP+YS+IVV FN 
Sbjct: 833  SPLFRLKTANTIQERVRFHNTGPSWVCGVIVMSIEDGHNGFPGLSQLDPIYSFIVVVFNA 892

Query: 2741 LQNEVLFTSHALQARNLVLHPVQLMSSDKLVMESNYDASSGSFTVPPRTTSVFVEPR 2911
               EV F S ALQ RNL LHP+Q+ SSD LV  S Y+ASSG F VP RTT+VFVEPR
Sbjct: 893  SPKEVSFVSPALQLRNLQLHPIQVSSSDDLVKSSRYEASSGCFVVPRRTTAVFVEPR 949