BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00009662
         (1427 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_850957.1| holocarboxylase synthetase 2 [Arabidopsis thali...   452   e-124
ref|XP_002893926.1| holocarboxylase synthetase 2 [Arabidopsis ly...   451   e-124
ref|XP_002878877.1| holocarboxylase synthetase 1 [Arabidopsis ly...   451   e-124
ref|XP_002301010.1| predicted protein [Populus trichocarpa] gi|2...   449   e-124
ref|NP_565605.1| holocarboxylase synthase 1 [Arabidopsis thalian...   449   e-123

>ref|NP_850957.1| holocarboxylase synthetase 2 [Arabidopsis thaliana]
            gi|332193776|gb|AEE31897.1| holocarboxylase synthetase 2
            [Arabidopsis thaliana]
          Length = 329

 Score =  452 bits (1163), Expect = e-124
 Identities = 218/324 (67%), Positives = 275/324 (84%), Gaps = 2/324 (0%)
 Frame = +3

Query: 138  LILHGKSSFETELAQSLKTNNTIKLPEEDAQLSILVHSSEQRGVQND--AFDVDLYMNSL 311
            L+L+GKSS ET+ A  LK NN +KLP+ ++++SI + S  +  V++D  +F++ L+MNS+
Sbjct: 9    LVLYGKSSVETDTATRLKNNNVLKLPD-NSKVSIFLQSEIKNLVRDDDSSFNLSLFMNSI 67

Query: 312  TTHRYGKFFIYSPRLSSTQDIVSKNFTEIPVGSVCVADLQFKGRGRSKNLWESPKGCLLF 491
            +THR+G+F I+SP LSST D+VS NF+EIPVGSVCV+D+Q KGRGR+KN+WESPKGCL++
Sbjct: 68   STHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWESPKGCLMY 127

Query: 492  SFTVEMEDGRVVPLLQYVVCLAMVEAIKDVCDVKGFAFIDVRIKWPNDLYLNGVKVGGIL 671
            SFT+EMEDGRVVPL+QYVV LA+ EA+KDVCD KG ++ DV+IKWPNDLYLNG+K+GGIL
Sbjct: 128  SFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNGLKIGGIL 187

Query: 672  CTSTYRSKKFNVSVGIGLNVDNEMPTTCLNKVLSELSMMDCKLRREEILAAFFKRFENFY 851
            CTSTYRS+KF VSVG+GLNVDNE PTTCLN VL ++      L+REEIL AFFK+FENF+
Sbjct: 188  CTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFFKKFENFF 247

Query: 852  DVFINQGFRALEEMYCRVWLHSGQRVVIQEKNNEDESADEVAVTVQGLTSSGYLLAIDDD 1031
            D+F+ QGF++LEE+Y R WLHSGQRV+ +EK NED+    V VT+QGLTSSGYLLAI DD
Sbjct: 248  DLFMEQGFKSLEELYYRTWLHSGQRVIAEEK-NEDQVVQNV-VTIQGLTSSGYLLAIGDD 305

Query: 1032 NQLYELHPDGNSFDFFKGLVRKKL 1103
            N +YELHPDGNSFDFFKGLVR+KL
Sbjct: 306  NVMYELHPDGNSFDFFKGLVRRKL 329


>ref|XP_002893926.1| holocarboxylase synthetase 2 [Arabidopsis lyrata subsp. lyrata]
            gi|297339768|gb|EFH70185.1| holocarboxylase synthetase 2
            [Arabidopsis lyrata subsp. lyrata]
          Length = 328

 Score =  451 bits (1161), Expect = e-124
 Identities = 220/323 (68%), Positives = 272/323 (84%), Gaps = 1/323 (0%)
 Frame = +3

Query: 138  LILHGKSSFETELAQSLKTNNTIKLPEEDAQLSILVHSSEQRGVQND-AFDVDLYMNSLT 314
            L+L GKSS ET+ A  LK NN +KLP+ + ++SI + S  +  V++D +F++ L+MNS++
Sbjct: 9    LVLCGKSSVETDAATRLKNNNVLKLPD-NTKVSIFLQSEIKNLVKDDDSFNLSLFMNSIS 67

Query: 315  THRYGKFFIYSPRLSSTQDIVSKNFTEIPVGSVCVADLQFKGRGRSKNLWESPKGCLLFS 494
            THR+G+F I+SP LSST D+VS NF+EIPVGSVCV D+QFKGRGR+KN+WESPKGCL++S
Sbjct: 68   THRFGRFLIWSPFLSSTHDVVSHNFSEIPVGSVCVTDIQFKGRGRTKNVWESPKGCLMYS 127

Query: 495  FTVEMEDGRVVPLLQYVVCLAMVEAIKDVCDVKGFAFIDVRIKWPNDLYLNGVKVGGILC 674
            FTVEMEDGRVVPL+QYVV LA+ EA+KDVCD KG ++IDV+IKWPNDLYLNG+KVGGILC
Sbjct: 128  FTVEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYIDVKIKWPNDLYLNGLKVGGILC 187

Query: 675  TSTYRSKKFNVSVGIGLNVDNEMPTTCLNKVLSELSMMDCKLRREEILAAFFKRFENFYD 854
            TSTY S+KF+VSVG+GLNVDNE PTTCLN VL E       L+REEIL AFF +FE F+D
Sbjct: 188  TSTYGSRKFHVSVGVGLNVDNEKPTTCLNAVLKENCPASNLLKREEILGAFFHKFETFFD 247

Query: 855  VFINQGFRALEEMYCRVWLHSGQRVVIQEKNNEDESADEVAVTVQGLTSSGYLLAIDDDN 1034
            +F+ QGF++LEE+Y R WLHSGQRV+++EK NED+    V VT+QGLTSSGYLLAI DDN
Sbjct: 248  LFMEQGFKSLEELYYRTWLHSGQRVIVEEK-NEDQVVQNV-VTIQGLTSSGYLLAIGDDN 305

Query: 1035 QLYELHPDGNSFDFFKGLVRKKL 1103
             +YELHPDGNSFDFFKGLVR+KL
Sbjct: 306  VMYELHPDGNSFDFFKGLVRRKL 328


>ref|XP_002878877.1| holocarboxylase synthetase 1 [Arabidopsis lyrata subsp. lyrata]
            gi|297324716|gb|EFH55136.1| holocarboxylase synthetase 1
            [Arabidopsis lyrata subsp. lyrata]
          Length = 365

 Score =  451 bits (1160), Expect = e-124
 Identities = 216/323 (66%), Positives = 274/323 (84%), Gaps = 1/323 (0%)
 Frame = +3

Query: 138  LILHGKSSFETELAQSLKTNNTIKLPEEDAQLSILVHSSEQRGVQND-AFDVDLYMNSLT 314
            L+L+GKSS ETE+A+ LK  N++ LP+ + ++S+ + S  +  V++D +F++ L+MNS+ 
Sbjct: 46   LVLYGKSSVETEIAKCLKNKNSLTLPD-NTKVSLFLESEAKNLVKDDGSFNLSLFMNSII 104

Query: 315  THRYGKFFIYSPRLSSTQDIVSKNFTEIPVGSVCVADLQFKGRGRSKNLWESPKGCLLFS 494
            THR+G+F I+SPRLSST D+VS+NF+E+PVGSVCV D+QFKGRGR+KN+WESPKGCL++S
Sbjct: 105  THRFGRFLIWSPRLSSTHDVVSQNFSELPVGSVCVTDIQFKGRGRTKNVWESPKGCLMYS 164

Query: 495  FTVEMEDGRVVPLLQYVVCLAMVEAIKDVCDVKGFAFIDVRIKWPNDLYLNGVKVGGILC 674
            FT+EMEDGRVVPL+QYVV LA+ EA+KDVCD KG  +IDV+IKWPNDLYLNG+KVGGILC
Sbjct: 165  FTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLYLNGLKVGGILC 224

Query: 675  TSTYRSKKFNVSVGIGLNVDNEMPTTCLNKVLSELSMMDCKLRREEILAAFFKRFENFYD 854
            TSTYRSKKF+VSVG+GLNVDNE PTTCLN VL  +S     L+REEIL AFF +FE F+D
Sbjct: 225  TSTYRSKKFHVSVGVGLNVDNEQPTTCLNAVLKGMSPESTLLKREEILGAFFHKFEKFFD 284

Query: 855  VFINQGFRALEEMYCRVWLHSGQRVVIQEKNNEDESADEVAVTVQGLTSSGYLLAIDDDN 1034
            +F++QGF++LEE+Y R WLHS QRV ++EK  ED+    V VT+QGL SSGYLLAI DDN
Sbjct: 285  LFMDQGFKSLEELYYRTWLHSEQRVTVEEK-VEDQVVQNV-VTIQGLASSGYLLAIGDDN 342

Query: 1035 QLYELHPDGNSFDFFKGLVRKKL 1103
            Q+YELHPDGNSFDFFKGLVR+K+
Sbjct: 343  QMYELHPDGNSFDFFKGLVRRKI 365


>ref|XP_002301010.1| predicted protein [Populus trichocarpa] gi|222842736|gb|EEE80283.1|
            predicted protein [Populus trichocarpa]
          Length = 333

 Score =  449 bits (1156), Expect = e-124
 Identities = 221/326 (67%), Positives = 269/326 (82%), Gaps = 3/326 (0%)
 Frame = +3

Query: 138  LILHGKSSFETELAQSLKTNNTIKLPEEDAQLSILVHS---SEQRGVQNDAFDVDLYMNS 308
            L+L GKSS E E+A+SL  NNT+KLP+ + Q+S L+HS   ++Q+    ++F ++ +MNS
Sbjct: 11   LVLCGKSSAENEIAKSLMNNNTLKLPD-NVQVSTLLHSEIINKQQRQDEESFCIERFMNS 69

Query: 309  LTTHRYGKFFIYSPRLSSTQDIVSKNFTEIPVGSVCVADLQFKGRGRSKNLWESPKGCLL 488
            L+T+++G+  I+SP L ST D+VS NF E+P+G+VCVAD+Q+KGRGRSKN+WESP GCL+
Sbjct: 70   LSTNQFGRLLIWSPILPSTHDVVSNNFGELPIGTVCVADVQYKGRGRSKNVWESPAGCLM 129

Query: 489  FSFTVEMEDGRVVPLLQYVVCLAMVEAIKDVCDVKGFAFIDVRIKWPNDLYLNGVKVGGI 668
            FSFT++MEDGRVVPLLQYVV LA+ EAIKDVCD  G   IDVRIKWPNDLYLNG+KVGGI
Sbjct: 130  FSFTIQMEDGRVVPLLQYVVSLAVTEAIKDVCDKNGLPRIDVRIKWPNDLYLNGLKVGGI 189

Query: 669  LCTSTYRSKKFNVSVGIGLNVDNEMPTTCLNKVLSELSMMDCKLRREEILAAFFKRFENF 848
            L TSTY+SKKFNVS GIGLNVDNE PTTCLN VL ELS   C LRRE+I+AAF  +FENF
Sbjct: 190  LSTSTYKSKKFNVSTGIGLNVDNEKPTTCLNAVLRELSAAACTLRREDIVAAFLNKFENF 249

Query: 849  YDVFINQGFRALEEMYCRVWLHSGQRVVIQEKNNEDESADEVAVTVQGLTSSGYLLAIDD 1028
            YD+FIN GF+ LEE+Y + WLHSGQRV+IQEKN  +    E  VT+QGLTSSGYLLAI +
Sbjct: 250  YDLFINGGFQTLEELYYKTWLHSGQRVIIQEKN--ENQVVENVVTIQGLTSSGYLLAIGE 307

Query: 1029 DNQLYELHPDGNSFDFFKGLVRKKLE 1106
            DNQ+ ELHPDGNSFDFFKGLVR+KLE
Sbjct: 308  DNQMCELHPDGNSFDFFKGLVRRKLE 333


>ref|NP_565605.1| holocarboxylase synthase 1 [Arabidopsis thaliana]
            gi|30682866|ref|NP_850067.1| holocarboxylase synthase 1
            [Arabidopsis thaliana] gi|1688072|gb|AAC49706.1| biotin
            holocarboxylase synthetase [Arabidopsis thaliana]
            gi|20197509|gb|AAD31371.2| biotin holocarboxylase
            synthetase [Arabidopsis thaliana]
            gi|89000993|gb|ABD59086.1| At2g25710 [Arabidopsis
            thaliana] gi|330252646|gb|AEC07740.1| holocarboxylase
            synthase 1 [Arabidopsis thaliana]
            gi|330252647|gb|AEC07741.1| holocarboxylase synthase 1
            [Arabidopsis thaliana]
          Length = 367

 Score =  449 bits (1154), Expect = e-123
 Identities = 214/324 (66%), Positives = 275/324 (84%), Gaps = 2/324 (0%)
 Frame = +3

Query: 138  LILHGKSSFETELAQSLKTNNTIKLPEEDAQLSILVHSSEQRGVQND--AFDVDLYMNSL 311
            L+L GKSS ETE+A+ LK  N++KLP+ + ++S+++ S  +  V++D  +F++ L+MNS+
Sbjct: 47   LVLCGKSSVETEVAKGLKNKNSLKLPD-NTKVSLILESEAKNLVKDDDNSFNLSLFMNSI 105

Query: 312  TTHRYGKFFIYSPRLSSTQDIVSKNFTEIPVGSVCVADLQFKGRGRSKNLWESPKGCLLF 491
             THR+G+F I+SPRLSST D+VS NF+E+PVGSVCV D+QFKGRGR+KN+WESPKGCL++
Sbjct: 106  ITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGRTKNVWESPKGCLMY 165

Query: 492  SFTVEMEDGRVVPLLQYVVCLAMVEAIKDVCDVKGFAFIDVRIKWPNDLYLNGVKVGGIL 671
            SFT+EMEDGRVVPL+QYVV LA+ EA+KDVCD KG  +IDV+IKWPNDLY+NG+KVGGIL
Sbjct: 166  SFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLYVNGLKVGGIL 225

Query: 672  CTSTYRSKKFNVSVGIGLNVDNEMPTTCLNKVLSELSMMDCKLRREEILAAFFKRFENFY 851
            CTSTYRSKKFNVSVG+GLNVDN  PTTCLN VL  ++     L+REEIL AFF +FE F+
Sbjct: 226  CTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEILGAFFHKFEKFF 285

Query: 852  DVFINQGFRALEEMYCRVWLHSGQRVVIQEKNNEDESADEVAVTVQGLTSSGYLLAIDDD 1031
            D+F++QGF++LEE+Y R WLHS QRV++++K  ED+    V VT+QGLTSSGYLLA+ DD
Sbjct: 286  DLFMDQGFKSLEELYYRTWLHSEQRVIVEDK-VEDQVVQNV-VTIQGLTSSGYLLAVGDD 343

Query: 1032 NQLYELHPDGNSFDFFKGLVRKKL 1103
            NQ+YELHPDGNSFDFFKGLVR+K+
Sbjct: 344  NQMYELHPDGNSFDFFKGLVRRKI 367