BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00008221
         (2203 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002274128.1| PREDICTED: sterol 14-demethylase [Vitis vini...   565   e-158
ref|XP_002299356.1| cytochrome P450 obtusifoliol 14-alpha-demeth...   561   e-157
gb|ABC59074.1| cytochrome P450 monooxygenase CYP51G1 [Medicago t...   559   e-156
ref|XP_002303744.1| cytochrome P450 probable obtusifoliol 14-alp...   556   e-155
gb|ACE06982.1| obtusifoliol-14alpha-demethylase [Gossypium hirsu...   555   e-155

>ref|XP_002274128.1| PREDICTED: sterol 14-demethylase [Vitis vinifera]
          Length = 486

 Score =  565 bits (1455), Expect = e-158
 Identities = 281/342 (82%), Positives = 301/342 (88%)
 Frame = -3

Query: 1214 FCXQMVVEAEDYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDL 1035
            +  QMV+EAEDYFSKWGD GEVDLKYELEHLIILTASRCLLG+E+R+KLF DVSALFHDL
Sbjct: 145  YVDQMVMEAEDYFSKWGDCGEVDLKYELEHLIILTASRCLLGQEIRNKLFADVSALFHDL 204

Query: 1034 DSGMLPISVIFPYLPIPAHRRRDQARKKLSEIFAKIISSRKETGKSENDMLQCFIESKYK 855
            D+GMLPISVIFPYLPIPAHRRRDQARKKL+EIFA II+SRKETGKSENDMLQCFI SKYK
Sbjct: 205  DNGMLPISVIFPYLPIPAHRRRDQARKKLAEIFANIIASRKETGKSENDMLQCFIASKYK 264

Query: 854  DGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLQNKNCLSEVLDEQKTLMKKYGDN 675
            DGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLL++K  LS V DEQ++LMKKYG  
Sbjct: 265  DGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYLSAVQDEQRSLMKKYGSK 324

Query: 674  VDHDILSEMDVLYRSIKEAXXXXXXXXXXXXXXHSDFSVTTREGKEYDIPKGHIVAASPA 495
            VDHDILSEMDVLYR IKEA              H+DFSVTTR+GKEYDIPKGHIVA SPA
Sbjct: 325  VDHDILSEMDVLYRCIKEALRLHPPLIMLLRSSHTDFSVTTRDGKEYDIPKGHIVATSPA 384

Query: 494  FANRLPHIFKDPDVYDPNRFAAGREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWS 315
            FANRLPHI+KDPD YDP+RFA GREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWS
Sbjct: 385  FANRLPHIYKDPDRYDPDRFAVGREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWS 444

Query: 314  HLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYKRRQLSLD 189
            HLLRNFELELISPFPE+DWNA          VRYKRR+L ++
Sbjct: 445  HLLRNFELELISPFPEVDWNAMVVGVKGKVMVRYKRRELPVN 486



 Score =  237 bits (605), Expect = 9e-60
 Identities = 115/151 (76%), Positives = 130/151 (86%)
 Frame = -2

Query: 2148 DNKFMSVGXXXXXXXXXXXLVSTLLIPRSKKRLPPIVKAWPVLGGLVRFMKGPVVMLREE 1969
            DNKF +             L+S L+IPRSKKRLPP +KA+P++GGL+RF+KGPVVMLREE
Sbjct: 4    DNKFFNAAFLLVATLVVAKLISALIIPRSKKRLPPTIKAFPLIGGLIRFLKGPVVMLREE 63

Query: 1968 YPKLGSVFTLNFLNKNITFFIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 1789
            YPKLGSVFTL  LNKNI+FFIGP+VSAHFFKA ESDLSQQEVY+FNVP FGPGVVFDVDY
Sbjct: 64   YPKLGSVFTLKLLNKNISFFIGPDVSAHFFKAPESDLSQQEVYRFNVPIFGPGVVFDVDY 123

Query: 1788 TIRQEQFRFFTEALRVNKLKGYVDQMVVEAE 1696
            ++RQEQFRFFTEALRV KLKGYVDQMV+EAE
Sbjct: 124  SVRQEQFRFFTEALRVTKLKGYVDQMVMEAE 154


>ref|XP_002299356.1| cytochrome P450 obtusifoliol 14-alpha-demethylase [Populus
            trichocarpa] gi|222846614|gb|EEE84161.1| cytochrome P450
            obtusifoliol 14-alpha-demethylase [Populus trichocarpa]
          Length = 488

 Score =  561 bits (1446), Expect = e-157
 Identities = 279/342 (81%), Positives = 300/342 (87%)
 Frame = -3

Query: 1214 FCXQMVVEAEDYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDL 1035
            +  QMVVEAEDYF KWGDSG VDLKYELEHLIILTASRCLLGREVRDKLFDDV+ALFHDL
Sbjct: 147  YVDQMVVEAEDYFLKWGDSGVVDLKYELEHLIILTASRCLLGREVRDKLFDDVAALFHDL 206

Query: 1034 DSGMLPISVIFPYLPIPAHRRRDQARKKLSEIFAKIISSRKETGKSENDMLQCFIESKYK 855
            D+GMLP+SV+FPYLPIPAHRRRD+ARKKL+EIFA II+SRK   KSENDMLQCFI+SKYK
Sbjct: 207  DNGMLPVSVLFPYLPIPAHRRRDRARKKLAEIFANIINSRKLASKSENDMLQCFIDSKYK 266

Query: 854  DGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLQNKNCLSEVLDEQKTLMKKYGDN 675
            DGRPTTESE+TGLLIAALFAGQHTSSITSTWTGAYLL++   LS VL+EQK LMKK+G+ 
Sbjct: 267  DGRPTTESEITGLLIAALFAGQHTSSITSTWTGAYLLRHNEYLSAVLEEQKNLMKKHGNK 326

Query: 674  VDHDILSEMDVLYRSIKEAXXXXXXXXXXXXXXHSDFSVTTREGKEYDIPKGHIVAASPA 495
            VDHDILSEMDVLYR IKEA              HSDFSVTTR+GKEYDIPKGHIVA SPA
Sbjct: 327  VDHDILSEMDVLYRCIKEALRLHPPLIMLLRSSHSDFSVTTRDGKEYDIPKGHIVATSPA 386

Query: 494  FANRLPHIFKDPDVYDPNRFAAGREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWS 315
            FANRLPH+FKDPD YDP+RFA GREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWS
Sbjct: 387  FANRLPHVFKDPDSYDPDRFAYGREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWS 446

Query: 314  HLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYKRRQLSLD 189
            HLLRNFELELISPFPEIDWNA          VRYKRR+LS++
Sbjct: 447  HLLRNFELELISPFPEIDWNAMVVGVKDKVMVRYKRRELSVN 488



 Score =  244 bits (624), Expect = 5e-62
 Identities = 116/151 (76%), Positives = 134/151 (88%)
 Frame = -2

Query: 2148 DNKFMSVGXXXXXXXXXXXLVSTLLIPRSKKRLPPIVKAWPVLGGLVRFMKGPVVMLREE 1969
            DNKF++VG           L+S L++PRS+KRLPP++K WP++GGL+RF+KGP+VMLREE
Sbjct: 6    DNKFLNVGLLIIATLLVAKLISALIMPRSQKRLPPVMKGWPLIGGLIRFLKGPIVMLREE 65

Query: 1968 YPKLGSVFTLNFLNKNITFFIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 1789
            YPKLGSVFT+N +N+ ITF IGPEVSAHFFKASE DLSQQEVYQFNVPTFGPGVVFDV+Y
Sbjct: 66   YPKLGSVFTVNLVNRKITFLIGPEVSAHFFKASEVDLSQQEVYQFNVPTFGPGVVFDVEY 125

Query: 1788 TIRQEQFRFFTEALRVNKLKGYVDQMVVEAE 1696
            +IRQEQFRFFTEALRVNKLKGYVDQMVVEAE
Sbjct: 126  SIRQEQFRFFTEALRVNKLKGYVDQMVVEAE 156


>gb|ABC59074.1| cytochrome P450 monooxygenase CYP51G1 [Medicago truncatula]
          Length = 489

 Score =  559 bits (1441), Expect = e-156
 Identities = 280/342 (81%), Positives = 297/342 (86%)
 Frame = -3

Query: 1214 FCXQMVVEAEDYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDL 1035
            +  QMV EAEDYFSKWG SGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDL
Sbjct: 147  YVNQMVSEAEDYFSKWGSSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDL 206

Query: 1034 DSGMLPISVIFPYLPIPAHRRRDQARKKLSEIFAKIISSRKETGKSENDMLQCFIESKYK 855
            D+GMLPISV+FPYLPIPAHRRRD ARKKL+EIFA IISSRK   KSE+DMLQCFI+SKYK
Sbjct: 207  DNGMLPISVLFPYLPIPAHRRRDNARKKLAEIFANIISSRKIADKSEDDMLQCFIDSKYK 266

Query: 854  DGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLQNKNCLSEVLDEQKTLMKKYGDN 675
            DGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLL NK  LS V +EQK LM+K+GD 
Sbjct: 267  DGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLCNKQYLSAVEEEQKNLMEKHGDR 326

Query: 674  VDHDILSEMDVLYRSIKEAXXXXXXXXXXXXXXHSDFSVTTREGKEYDIPKGHIVAASPA 495
            VDHD+L+EMDVLYR IKEA              HSDFSVTTREGKEYDIPKGHIVA SPA
Sbjct: 327  VDHDVLAEMDVLYRCIKEALRLHPPLIMLLRSSHSDFSVTTREGKEYDIPKGHIVATSPA 386

Query: 494  FANRLPHIFKDPDVYDPNRFAAGREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWS 315
            FANRLPHIF DPD YDP+RFA GREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIW+
Sbjct: 387  FANRLPHIFNDPDKYDPDRFAVGREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWT 446

Query: 314  HLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYKRRQLSLD 189
            HLLRNFELEL+SPFPEIDWNA          VRYKRR+LS++
Sbjct: 447  HLLRNFELELVSPFPEIDWNAMVVGVKGKVMVRYKRRELSVN 488



 Score =  228 bits (581), Expect = 5e-57
 Identities = 108/150 (72%), Positives = 127/150 (84%)
 Frame = -2

Query: 2145 NKFMSVGXXXXXXXXXXXLVSTLLIPRSKKRLPPIVKAWPVLGGLVRFMKGPVVMLREEY 1966
            NKF++             L+S+ +IP+SKKRLPPI++ WP++GGL+RF+KGP+++LREEY
Sbjct: 7    NKFLNTLLLLITTLIAAKLISSFIIPKSKKRLPPILQGWPIIGGLLRFLKGPIILLREEY 66

Query: 1965 PKLGSVFTLNFLNKNITFFIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYT 1786
            PKLG VFTL   +KNITF IGPEVSAHFFKA ESDLSQQEVYQFNVPTFGPGVVFDVDY+
Sbjct: 67   PKLGGVFTLKIFHKNITFLIGPEVSAHFFKAPESDLSQQEVYQFNVPTFGPGVVFDVDYS 126

Query: 1785 IRQEQFRFFTEALRVNKLKGYVDQMVVEAE 1696
            +RQEQFRFFTEALRVNKLK YV+QMV EAE
Sbjct: 127  VRQEQFRFFTEALRVNKLKSYVNQMVSEAE 156


>ref|XP_002303744.1| cytochrome P450 probable obtusifoliol 14-alpha-demethylase [Populus
            trichocarpa] gi|222841176|gb|EEE78723.1| cytochrome P450
            probable obtusifoliol 14-alpha-demethylase [Populus
            trichocarpa]
          Length = 488

 Score =  556 bits (1432), Expect = e-155
 Identities = 277/342 (80%), Positives = 299/342 (87%)
 Frame = -3

Query: 1214 FCXQMVVEAEDYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDL 1035
            +  QMVVEAEDYFSKWGDSG VD+KYELEHLIILTASRCLLGREVRDKLFDDVSALFHDL
Sbjct: 147  YVDQMVVEAEDYFSKWGDSGVVDIKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDL 206

Query: 1034 DSGMLPISVIFPYLPIPAHRRRDQARKKLSEIFAKIISSRKETGKSENDMLQCFIESKYK 855
            D+GMLPISV+FPYLPIPAHRRRD+ARKKL+EIFA II+SRK  GKSENDMLQCFI+SKYK
Sbjct: 207  DNGMLPISVLFPYLPIPAHRRRDRARKKLAEIFANIINSRKLAGKSENDMLQCFIDSKYK 266

Query: 854  DGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLQNKNCLSEVLDEQKTLMKKYGDN 675
            DGRPTTESE+TGLLIAALFAGQHTSSITSTWTGAYLL++   LS VL+EQK LMKK+G+ 
Sbjct: 267  DGRPTTESEITGLLIAALFAGQHTSSITSTWTGAYLLRHNEYLSAVLEEQKNLMKKHGNK 326

Query: 674  VDHDILSEMDVLYRSIKEAXXXXXXXXXXXXXXHSDFSVTTREGKEYDIPKGHIVAASPA 495
            VD DILSEM VL+R IKEA              HSDFSVTTR+GKEYDIPKGHIVA SPA
Sbjct: 327  VDQDILSEMGVLHRCIKEALRLHPPLIMLLRSSHSDFSVTTRDGKEYDIPKGHIVATSPA 386

Query: 494  FANRLPHIFKDPDVYDPNRFAAGREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWS 315
            FANRLPH+FKDP+ YDP+RFAAGREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWS
Sbjct: 387  FANRLPHVFKDPERYDPDRFAAGREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWS 446

Query: 314  HLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYKRRQLSLD 189
            HLLRNFE ELISPFPE DWNA          VRYKRR+LS++
Sbjct: 447  HLLRNFEFELISPFPETDWNAMVVGVKDKVMVRYKRRELSVN 488



 Score =  241 bits (616), Expect = 5e-61
 Identities = 115/151 (76%), Positives = 133/151 (88%)
 Frame = -2

Query: 2148 DNKFMSVGXXXXXXXXXXXLVSTLLIPRSKKRLPPIVKAWPVLGGLVRFMKGPVVMLREE 1969
            DNKF++VG           L+S L++PRS+KRLPP++K WP++GGL+RF+KGP+VMLREE
Sbjct: 6    DNKFLNVGLLILATLLVAKLISALIMPRSQKRLPPVMKGWPLIGGLIRFLKGPIVMLREE 65

Query: 1968 YPKLGSVFTLNFLNKNITFFIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 1789
            YPKLGSVFT+N  N  ITF IGPEVSAHFFKASE+DLSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 66   YPKLGSVFTVNLANWKITFLIGPEVSAHFFKASEADLSQQEVYQFNVPTFGPGVVFDVDY 125

Query: 1788 TIRQEQFRFFTEALRVNKLKGYVDQMVVEAE 1696
            +IRQEQFRFFTE+LRV+KLKGYVDQMVVEAE
Sbjct: 126  SIRQEQFRFFTESLRVSKLKGYVDQMVVEAE 156


>gb|ACE06982.1| obtusifoliol-14alpha-demethylase [Gossypium hirsutum]
          Length = 486

 Score =  555 bits (1431), Expect = e-155
 Identities = 275/342 (80%), Positives = 298/342 (87%)
 Frame = -3

Query: 1214 FCXQMVVEAEDYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDL 1035
            +  QMV EAEDYFSKWGDSGEVDLKYELEHLIILTASRCLLG+EVR+KLFDDVSALFHDL
Sbjct: 145  YVDQMVTEAEDYFSKWGDSGEVDLKYELEHLIILTASRCLLGQEVRNKLFDDVSALFHDL 204

Query: 1034 DSGMLPISVIFPYLPIPAHRRRDQARKKLSEIFAKIISSRKETGKSENDMLQCFIESKYK 855
            D+GMLPISVIFPYLPIPAHRRRD+ARKKL+ IFA II+SRK  GKSENDMLQCFIESKYK
Sbjct: 205  DNGMLPISVIFPYLPIPAHRRRDRARKKLAGIFANIIASRKSAGKSENDMLQCFIESKYK 264

Query: 854  DGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLQNKNCLSEVLDEQKTLMKKYGDN 675
            DGRPTTE+EVTGLLIAALFAGQHTSSITSTWTGAYLL++K  LS V++EQK +M+K+G  
Sbjct: 265  DGRPTTEAEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEFLSAVIEEQKQIMQKHGSK 324

Query: 674  VDHDILSEMDVLYRSIKEAXXXXXXXXXXXXXXHSDFSVTTREGKEYDIPKGHIVAASPA 495
            VDHDILSEMD LYR IKEA              HSDFSV TR+GKEYDIPKGHIVA SPA
Sbjct: 325  VDHDILSEMDTLYRCIKEALRLHPPLIMLLRSSHSDFSVKTRDGKEYDIPKGHIVATSPA 384

Query: 494  FANRLPHIFKDPDVYDPNRFAAGREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWS 315
            FANRLP+I+KDPD YDP+RF+ GREEDK AGAFSYISFGGGRHGCLGEPFAYLQIKAIWS
Sbjct: 385  FANRLPYIYKDPDTYDPDRFSVGREEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWS 444

Query: 314  HLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYKRRQLSLD 189
            HLLRNFELEL+SPFPEIDWNA          VRYKRRQLS++
Sbjct: 445  HLLRNFELELVSPFPEIDWNAMVVGVKGKVMVRYKRRQLSVN 486



 Score =  242 bits (618), Expect = 3e-61
 Identities = 118/151 (78%), Positives = 130/151 (86%)
 Frame = -2

Query: 2148 DNKFMSVGXXXXXXXXXXXLVSTLLIPRSKKRLPPIVKAWPVLGGLVRFMKGPVVMLREE 1969
            ++K ++ G           L+S L++PRSKKR+PP VK WPV+GGL+RFMKGP+VMLREE
Sbjct: 4    NDKLLNTGILIVATLVVAKLISFLIMPRSKKRVPPAVKTWPVIGGLLRFMKGPIVMLREE 63

Query: 1968 YPKLGSVFTLNFLNKNITFFIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 1789
            YPKLGSVFTLN  NK ITF IGPEVS+HFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 64   YPKLGSVFTLNLFNKKITFLIGPEVSSHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 123

Query: 1788 TIRQEQFRFFTEALRVNKLKGYVDQMVVEAE 1696
            TIRQEQFRFFTEALRVNKLKGYVDQMV EAE
Sbjct: 124  TIRQEQFRFFTEALRVNKLKGYVDQMVTEAE 154