BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00008007
         (1703 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI16383.3| unnamed protein product [Vitis vinifera]              721   0.0  
ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnm...   721   0.0  
ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   709   0.0  
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   696   0.0  
ref|XP_002298404.1| predicted protein [Populus trichocarpa] gi|2...   674   0.0  

>emb|CBI16383.3| unnamed protein product [Vitis vinifera]
          Length = 544

 Score =  721 bits (1860), Expect = 0.0
 Identities = 349/470 (74%), Positives = 399/470 (84%), Gaps = 12/470 (2%)
 Frame = +3

Query: 204  YLTCSMPGKKKLKIALLLSGGVDSSVALRLLHHAGHSCTAFYLKIWFQEDYENFWAACPW 383
            YL+CS+P  + LKIA+LLSGGVDSSVALRLLH AGHSCTAFYLKIWFQED+ENFW+ CPW
Sbjct: 78   YLSCSLPNNR-LKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 136

Query: 384  DEDLKYAEAVCDQIGVPLEVVHLTEEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF 563
            ++DLKYA+AVCDQ+ VPLEVVHLT+EYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF
Sbjct: 137  EDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF 196

Query: 564  MNAISSMEFDYVASGHYARVVHSSSEQDSGASVLELSNDTVKDQTYFLSHLSQDQLKRLL 743
            M+AISS EFDYVASGHYA VVHS ++Q    SVLELS D VKDQTYFLSHLSQ QLKRL+
Sbjct: 197  MDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQIQLKRLI 256

Query: 744  FPLGRISKDEVRRLATNFDLPNKERKDSQGICFLGKIKFSDFVARHIGELEGVLLEAESG 923
            FPLG I KDEVR LAT  DLPN++RKDSQGICFLGKIKFS+FVARHIGE EG++LEAE+G
Sbjct: 257  FPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETG 316

Query: 924  DFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIQNNVVFVSRNYYSLDKRRRKFRVGSL 1103
            D LGKHRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVSRNY+SLDKRRR FRVGSL
Sbjct: 317  DLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFRVGSL 376

Query: 1104 KWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQGLAAGQFAA 1283
            KW+S  PP   +QL CKVRHGP FY C+ TMEP +DG +DV +VQL EDDQGLAAGQF A
Sbjct: 377  KWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQGLAAGQFTA 436

Query: 1284 FYRDKNCLGSGIILESWEDQGFPVCARAQEIARLEDKSKLGKPVKIMVQPE--PK----- 1442
            FY+ ++C+GSG+ILESW+DQGFPVCA+A EIAR+EDKSKLGKPVKI V+PE  PK     
Sbjct: 437  FYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPETPPKGSDQI 496

Query: 1443 -----DREPAMAKVTPSEPVIETPTPVSQGIFSSCFPMNWLKELRNKWLR 1577
                 DRE A ++   ++ +  +    S+G   S FPMNWL+++R KWL+
Sbjct: 497  DGIKLDRELANSQAAVTDEISRS----SEGEARSRFPMNWLQKIREKWLQ 542


>ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Vitis vinifera]
          Length = 540

 Score =  721 bits (1860), Expect = 0.0
 Identities = 349/470 (74%), Positives = 399/470 (84%), Gaps = 12/470 (2%)
 Frame = +3

Query: 204  YLTCSMPGKKKLKIALLLSGGVDSSVALRLLHHAGHSCTAFYLKIWFQEDYENFWAACPW 383
            YL+CS+P  + LKIA+LLSGGVDSSVALRLLH AGHSCTAFYLKIWFQED+ENFW+ CPW
Sbjct: 74   YLSCSLPNNR-LKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 132

Query: 384  DEDLKYAEAVCDQIGVPLEVVHLTEEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF 563
            ++DLKYA+AVCDQ+ VPLEVVHLT+EYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF
Sbjct: 133  EDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF 192

Query: 564  MNAISSMEFDYVASGHYARVVHSSSEQDSGASVLELSNDTVKDQTYFLSHLSQDQLKRLL 743
            M+AISS EFDYVASGHYA VVHS ++Q    SVLELS D VKDQTYFLSHLSQ QLKRL+
Sbjct: 193  MDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQIQLKRLI 252

Query: 744  FPLGRISKDEVRRLATNFDLPNKERKDSQGICFLGKIKFSDFVARHIGELEGVLLEAESG 923
            FPLG I KDEVR LAT  DLPN++RKDSQGICFLGKIKFS+FVARHIGE EG++LEAE+G
Sbjct: 253  FPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETG 312

Query: 924  DFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIQNNVVFVSRNYYSLDKRRRKFRVGSL 1103
            D LGKHRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVSRNY+SLDKRRR FRVGSL
Sbjct: 313  DLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFRVGSL 372

Query: 1104 KWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQGLAAGQFAA 1283
            KW+S  PP   +QL CKVRHGP FY C+ TMEP +DG +DV +VQL EDDQGLAAGQF A
Sbjct: 373  KWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQGLAAGQFTA 432

Query: 1284 FYRDKNCLGSGIILESWEDQGFPVCARAQEIARLEDKSKLGKPVKIMVQPE--PK----- 1442
            FY+ ++C+GSG+ILESW+DQGFPVCA+A EIAR+EDKSKLGKPVKI V+PE  PK     
Sbjct: 433  FYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPETPPKGSDQI 492

Query: 1443 -----DREPAMAKVTPSEPVIETPTPVSQGIFSSCFPMNWLKELRNKWLR 1577
                 DRE A ++   ++ +  +    S+G   S FPMNWL+++R KWL+
Sbjct: 493  DGIKLDRELANSQAAVTDEISRS----SEGEARSRFPMNWLQKIREKWLQ 538


>ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cucumis sativus]
            gi|449524848|ref|XP_004169433.1| PREDICTED: tRNA-specific
            2-thiouridylase MnmA-like [Cucumis sativus]
          Length = 538

 Score =  709 bits (1830), Expect = 0.0
 Identities = 349/468 (74%), Positives = 391/468 (83%), Gaps = 8/468 (1%)
 Frame = +3

Query: 204  YLTCSMPGKKKLKIALLLSGGVDSSVALRLLHHAGHSCTAFYLKIWFQEDYENFWAACPW 383
            +L+CSMP    LK+A+LLSGGVDSSVALRLLH AGHSCTAFYLKIWFQED+ENFW+ CPW
Sbjct: 72   FLSCSMP-TYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 130

Query: 384  DEDLKYAEAVCDQIGVPLEVVHLTEEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF 563
            D+DLKYA+AVCDQ+ VPLEVVHLTEEYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF
Sbjct: 131  DDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF 190

Query: 564  MNAISSMEFDYVASGHYARVVHSSSEQDSGASVLELSNDTVKDQTYFLSHLSQDQLKRLL 743
            M+AIS+MEFDYVASGHYA V+H S++Q    SVLELS D VKDQTYFLSHLSQ+QLKRLL
Sbjct: 191  MDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLL 250

Query: 744  FPLGRISKDEVRRLATNFDLPNKERKDSQGICFLGKIKFSDFVARHIGELEGVLLEAESG 923
            FPLG I KDEVR+LA  F+LPNK+RKDSQGICFLGKIKFS+FVARHIGE EGV+LEAESG
Sbjct: 251  FPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESG 310

Query: 924  DFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIQNNVVFVSRNYYSLDKRRRKFRVGSL 1103
            DFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDI+NNVVFVSRNY+S DKRRR FRVGSL
Sbjct: 311  DFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSL 370

Query: 1104 KWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQGLAAGQFAA 1283
            KWLSG PPD F +L CKVRHGPSFY C LTME  +DG +DV +V L EDDQGLAAGQFAA
Sbjct: 371  KWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAA 430

Query: 1284 FYRDKNCLGSGIILESWEDQGFPVCARAQEIARLEDKSKLGKPVKIMVQPE-------PK 1442
            FY+ ++C+GSG+ILESW+DQGFPVC +A E A++EDKSKLGKP+KI V+PE        K
Sbjct: 431  FYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPESGMKESKAK 490

Query: 1443 DREPAMAKVTPSE-PVIETPTPVSQGIFSSCFPMNWLKELRNKWLRTF 1583
            D      K+   E  V+E    VS     +   M WL  L+ K L+ F
Sbjct: 491  DDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538


>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
            tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis]
          Length = 497

 Score =  696 bits (1795), Expect = 0.0
 Identities = 323/420 (76%), Positives = 373/420 (88%)
 Frame = +3

Query: 192  VEPCYLTCSMPGKKKLKIALLLSGGVDSSVALRLLHHAGHSCTAFYLKIWFQEDYENFWA 371
            +EP YL+C +P K  L+IALLLSGGVDSSVALRLLH AGH CTAFYLKIWFQED+ENFW+
Sbjct: 56   IEPNYLSCCLPDKNNLRIALLLSGGVDSSVALRLLHAAGHHCTAFYLKIWFQEDFENFWS 115

Query: 372  ACPWDEDLKYAEAVCDQIGVPLEVVHLTEEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIK 551
             CPW+EDLKYA+AVCDQ+ +PLEVVHLT+EYW NVVSYIIEEYRCGRTPNPDVLCNTRIK
Sbjct: 116  ECPWEEDLKYAKAVCDQVDIPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIK 175

Query: 552  FGAFMNAISSMEFDYVASGHYARVVHSSSEQDSGASVLELSNDTVKDQTYFLSHLSQDQL 731
            FGAFM+AI++MEFDYVASGHYA+V+H S+++ +  SVLELS D VKDQTYFLSHLSQ QL
Sbjct: 176  FGAFMDAINNMEFDYVASGHYAKVIHPSADEINQPSVLELSKDMVKDQTYFLSHLSQAQL 235

Query: 732  KRLLFPLGRISKDEVRRLATNFDLPNKERKDSQGICFLGKIKFSDFVARHIGELEGVLLE 911
            +RL+FPLG ISKDEVR+LAT F+LPNK+RKDSQGICFLGKIKFS+FVARHIGE+EG++LE
Sbjct: 236  RRLIFPLGGISKDEVRKLATLFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGIMLE 295

Query: 912  AESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIQNNVVFVSRNYYSLDKRRRKFR 1091
            AE+GDFLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVSRNY+S DKRRR  R
Sbjct: 296  AETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRLLR 355

Query: 1092 VGSLKWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQGLAAG 1271
            VGSL+W +GSPP    +L CKVRHGP FY C++ +EP +   +DV +VQL EDDQGLAAG
Sbjct: 356  VGSLRWFNGSPPKETSELQCKVRHGPGFYNCSVVIEPGEVDDQDVAIVQLCEDDQGLAAG 415

Query: 1272 QFAAFYRDKNCLGSGIILESWEDQGFPVCARAQEIARLEDKSKLGKPVKIMVQPEPKDRE 1451
            QFAAFY+ + C+GSG+ILESW DQGFPVC RA E+AR+EDKS+LGKPVKI V+PE   RE
Sbjct: 416  QFAAFYKGRTCIGSGVILESWNDQGFPVCERALELARIEDKSELGKPVKIKVKPEIPVRE 475


>ref|XP_002298404.1| predicted protein [Populus trichocarpa] gi|222845662|gb|EEE83209.1|
            predicted protein [Populus trichocarpa]
          Length = 434

 Score =  674 bits (1738), Expect = 0.0
 Identities = 317/407 (77%), Positives = 361/407 (88%)
 Frame = +3

Query: 231  KKLKIALLLSGGVDSSVALRLLHHAGHSCTAFYLKIWFQEDYENFWAACPWDEDLKYAEA 410
            K L+IA+LLSGGVDSSVALRLLH AGH CTAFYLKIWFQED+ NFW+ CPW+EDLK+A+ 
Sbjct: 6    KPLRIAVLLSGGVDSSVALRLLHAAGHHCTAFYLKIWFQEDFNNFWSECPWEEDLKFAKG 65

Query: 411  VCDQIGVPLEVVHLTEEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMNAISSMEF 590
            VCDQ+GVPLEVVHLT+EYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFM+AI++MEF
Sbjct: 66   VCDQVGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAINNMEF 125

Query: 591  DYVASGHYARVVHSSSEQDSGASVLELSNDTVKDQTYFLSHLSQDQLKRLLFPLGRISKD 770
            DYVASGHYA V+H  S+Q +  SVLELS D +KDQTYFLSHL+Q QLKRL+FPLG I K+
Sbjct: 126  DYVASGHYAHVIHPVSDQINKPSVLELSKDKLKDQTYFLSHLTQAQLKRLIFPLGCIPKE 185

Query: 771  EVRRLATNFDLPNKERKDSQGICFLGKIKFSDFVARHIGELEGVLLEAESGDFLGKHRGF 950
            EVR LA+ FDLPNK+RKDSQGICFLGKIKFS+FV RHIGE EGV+LEAE+GDFLG HRGF
Sbjct: 186  EVRNLASKFDLPNKDRKDSQGICFLGKIKFSEFVGRHIGESEGVILEAETGDFLGIHRGF 245

Query: 951  WFYTIGQRQGLRLPGGPWYVVEKDIQNNVVFVSRNYYSLDKRRRKFRVGSLKWLSGSPPD 1130
            WFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVSRNY+SLDKRRR FRVGSLKWLSGSP D
Sbjct: 246  WFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRSFRVGSLKWLSGSPTD 305

Query: 1131 HFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQGLAAGQFAAFYRDKNCLG 1310
               QL CKVRHGP+FY C++ +E  +D  ++V  V L EDDQGLAAGQF AFY+ + C+G
Sbjct: 306  MTTQLQCKVRHGPTFYDCSVKIELGEDSPEEVVAVHLCEDDQGLAAGQFTAFYQGRTCIG 365

Query: 1311 SGIILESWEDQGFPVCARAQEIARLEDKSKLGKPVKIMVQPEPKDRE 1451
            SG+ILESW+D+GFPVC +A E+ARLEDKSKLGKPVKI V+PE  D+E
Sbjct: 366  SGVILESWDDKGFPVCQKALELARLEDKSKLGKPVKIKVKPETSDQE 412