BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00005305
         (1089 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002314018.1| predicted protein [Populus trichocarpa] gi|2...   358   e-115
ref|XP_002267920.1| PREDICTED: cysteine desulfurase 2, chloropla...   405   e-111
ref|XP_002523229.1| cysteine desulfurylase, putative [Ricinus co...   392   e-106
ref|NP_172325.2| cysteine desulfurase 2 [Arabidopsis thaliana] g...   389   e-106
ref|XP_003546241.1| PREDICTED: cysteine desulfurase 2, chloropla...   389   e-106

>ref|XP_002314018.1| predicted protein [Populus trichocarpa] gi|222850426|gb|EEE87973.1|
           predicted protein [Populus trichocarpa]
          Length = 469

 Score =  358 bits (920), Expect(2) = e-115
 Identities = 179/224 (79%), Positives = 197/224 (87%)
 Frame = -3

Query: 676 QRRIKVARFINASESTEIVFTRNATEAMNLVAYSWGLLNLRPGDEIILTIAEHHSAIVPF 497
           Q R K+A FINA ES EIVFTRNATEA+NLVAY+WG  NL+ GDE++LTIAEHHSAIVP+
Sbjct: 126 QAREKIAAFINAEESREIVFTRNATEAINLVAYTWGFANLKSGDEVLLTIAEHHSAIVPW 185

Query: 496 QIVAQKTGATLKFVSLTEEEEVPDIEKLKEMLSEKTKLVVTHHVSNTLGSVLPIKDITSW 317
           Q VAQKTGA LKFV+L E+E VPD+ +LKEM+S KTKLVV HH+SNTL S LPI+DI  W
Sbjct: 186 QFVAQKTGAVLKFVNLNEDE-VPDVLQLKEMISRKTKLVVVHHISNTLASSLPIEDIVHW 244

Query: 316 SHNVGAKVLVDACQSVPHMVVDVQNLDADFLVASSHKMCGPTGAGFLYGKSGILSGMPPF 137
           +H V AKVLVDACQSVPHM VDV++LDADFLVASSHKMCGPTG GFLYGKS ILS MPPF
Sbjct: 245 AHAVEAKVLVDACQSVPHMPVDVRDLDADFLVASSHKMCGPTGVGFLYGKSDILSTMPPF 304

Query: 136 LAGGEMISDVFLDYSTYAEPPSRFEAGTPAIGEVIGLGAAIDYL 5
           L GGEMISDVFLD+STYAEPPSRFEAGTPAIGE IGLGAAIDYL
Sbjct: 305 LGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAIDYL 348



 Score = 85.5 bits (210), Expect(2) = e-115
 Identities = 43/69 (62%), Positives = 55/69 (79%)
 Frame = -2

Query: 857 LHSSLKNVNKSKLVYLDNAATSQKPTTAVLDSLQNYYSTYNSNVHRGIHFLSARATDEYE 678
           LH  + N   S LVYLDNAATSQKP + VL +LQ+YY +YNSNVHRGIH+LSA+AT++YE
Sbjct: 69  LHQKINN--GSNLVYLDNAATSQKPIS-VLKALQDYYESYNSNVHRGIHYLSAKATEKYE 125

Query: 677 SAKDKGGSF 651
            A++K  +F
Sbjct: 126 QAREKIAAF 134


>ref|XP_002267920.1| PREDICTED: cysteine desulfurase 2, chloroplastic [Vitis vinifera]
            gi|296083920|emb|CBI24308.3| unnamed protein product
            [Vitis vinifera]
          Length = 463

 Score =  405 bits (1041), Expect = e-111
 Identities = 219/356 (61%), Positives = 253/356 (71%), Gaps = 2/356 (0%)
 Frame = -3

Query: 1063 FRKFSCAATASSDIAVEKS--SLSLGHITRPDFPILHQNVNKSKLVYLDNXXXXXXXSGG 890
            FR+FSC+A +S+ +  + S  S+SLGH+TRPDFPILHQ VN SKLVYLDN          
Sbjct: 29   FRRFSCSAPSSAAVEGDSSIGSVSLGHLTRPDFPILHQEVNGSKLVYLDNA--------- 79

Query: 889  ILVTIVCTELPCILPSRM*TSQNWSTWIMQQLHKSLLLLFWTLYKTITQPIIPMCIVVFI 710
                   ++ P  +   +   QN+       +H+ +  L                     
Sbjct: 80   -----ATSQKPTAVLKAL---QNYYEAYNSNVHRGIHFLS-------------------- 111

Query: 709  S*VQEPLMNMNQRRIKVARFINASESTEIVFTRNATEAMNLVAYSWGLLNLRPGDEIILT 530
                +        R KVA FINASE  EI+FTRNATEA+NLVAYSWGL NL+P DEI+LT
Sbjct: 112  ---AKATDEYESARRKVAAFINASEPGEIIFTRNATEAINLVAYSWGLSNLKPEDEIVLT 168

Query: 529  IAEHHSAIVPFQIVAQKTGATLKFVSLTEEEEVPDIEKLKEMLSEKTKLVVTHHVSNTLG 350
            +AEHHSAIVP+Q+VAQKTGA LKFV+LTE+E VPD+EKLKEM+S KTKL+V HH+SN L 
Sbjct: 169  VAEHHSAIVPWQLVAQKTGAILKFVNLTEDE-VPDVEKLKEMISRKTKLLVVHHISNVLA 227

Query: 349  SVLPIKDITSWSHNVGAKVLVDACQSVPHMVVDVQNLDADFLVASSHKMCGPTGAGFLYG 170
            S LPI DI  W+H VGAKVLVDACQSVPHM VDVQ LDADFLVASSHKMCGPTG GFLYG
Sbjct: 228  SALPIYDIVHWAHAVGAKVLVDACQSVPHMAVDVQRLDADFLVASSHKMCGPTGIGFLYG 287

Query: 169  KSGILSGMPPFLAGGEMISDVFLDYSTYAEPPSRFEAGTPAIGEVIGLGAAIDYLS 2
            KS +L  MPPFL GGEMISDVFLD+ST+AEPPSRFEAGTPAIGE IGLGAAIDYLS
Sbjct: 288  KSDLLFAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPAIGEAIGLGAAIDYLS 343


>ref|XP_002523229.1| cysteine desulfurylase, putative [Ricinus communis]
            gi|223537525|gb|EEF39150.1| cysteine desulfurylase,
            putative [Ricinus communis]
          Length = 469

 Score =  392 bits (1006), Expect = e-106
 Identities = 213/364 (58%), Positives = 251/364 (68%), Gaps = 3/364 (0%)
 Frame = -3

Query: 1087 TTTVKVSKFRKFSCAATASSDIAVEKS---SLSLGHITRPDFPILHQNVNKSKLVYLDNX 917
            ++ ++++KF  F+  A A++    +++   SLSLGHITRPDFPILHQ VN SKLVYLDN 
Sbjct: 26   SSPIRLAKFSSFAIPALATTGTEGDQALHASLSLGHITRPDFPILHQEVNGSKLVYLDNA 85

Query: 916  XXXXXXSGGILVTIVCTELPCILPSRM*TSQNWSTWIMQQLHKSLLLLFWTLYKTITQPI 737
                  +  +L  I                QN+       +H+ +  L            
Sbjct: 86   ATSQKPNS-VLKAI----------------QNYYESYNSNVHRGIHYLS----------- 117

Query: 736  IPMCIVVFIS*VQEPLMNMNQRRIKVARFINASESTEIVFTRNATEAMNLVAYSWGLLNL 557
                         +        R KVA FINAS+S EIVFTRNATEA+NLVAYSWG  NL
Sbjct: 118  ------------AKATDEYELARKKVAAFINASDSREIVFTRNATEAINLVAYSWGFTNL 165

Query: 556  RPGDEIILTIAEHHSAIVPFQIVAQKTGATLKFVSLTEEEEVPDIEKLKEMLSEKTKLVV 377
            +PGDE+ILT+AEHHS IVP+Q+VA +TGA L+FV L E E VPD+ KLKE +SEKTKLVV
Sbjct: 166  KPGDEVILTLAEHHSNIVPWQLVANRTGAVLRFVDLNENE-VPDVLKLKETISEKTKLVV 224

Query: 376  THHVSNTLGSVLPIKDITSWSHNVGAKVLVDACQSVPHMVVDVQNLDADFLVASSHKMCG 197
             HH+SN L S LP++DI  W+H  GAKVL+DACQSVPHMVVDVQ LDADFLVASSHKMCG
Sbjct: 225  VHHISNVLASFLPVEDIIHWAHGAGAKVLIDACQSVPHMVVDVQGLDADFLVASSHKMCG 284

Query: 196  PTGAGFLYGKSGILSGMPPFLAGGEMISDVFLDYSTYAEPPSRFEAGTPAIGEVIGLGAA 17
            PTG GFLYGKS ILS MPPFL GGEMI+DVFLD+STYA+PPSRFEAGTPAIGE IGLGAA
Sbjct: 285  PTGIGFLYGKSDILSTMPPFLGGGEMIADVFLDHSTYADPPSRFEAGTPAIGEAIGLGAA 344

Query: 16   IDYL 5
            IDYL
Sbjct: 345  IDYL 348


>ref|NP_172325.2| cysteine desulfurase 2 [Arabidopsis thaliana]
            gi|75249321|sp|Q93WX6.1|NFS2_ARATH RecName: Full=Cysteine
            desulfurase 2, chloroplastic; AltName: Full=NIFS-like
            protein; AltName: Full=Plastid sufS-like protein;
            AltName: Full=Protein AtCpNifS; AltName:
            Full=Selenocysteine lyase; Flags: Precursor
            gi|16152176|gb|AAL14994.1|AF419347_1 NIFS-like protein
            CpNifsp precursor [Arabidopsis thaliana]
            gi|20453112|gb|AAM19798.1| At1g08490/T27G7_14
            [Arabidopsis thaliana] gi|23506185|gb|AAN31104.1|
            At1g08490/T27G7_14 [Arabidopsis thaliana]
            gi|27085243|gb|AAL79956.1| cysteine desulfurase
            [Arabidopsis thaliana] gi|332190177|gb|AEE28298.1|
            cysteine desulfurase 2 [Arabidopsis thaliana]
          Length = 463

 Score =  389 bits (998), Expect = e-106
 Identities = 210/350 (60%), Positives = 246/350 (70%)
 Frame = -3

Query: 1051 SCAATASSDIAVEKSSLSLGHITRPDFPILHQNVNKSKLVYLDNXXXXXXXSGGILVTIV 872
            S AA +S+ I+ +  S+SLGH  R DF ILHQ VN SKLVYLD+                
Sbjct: 35   SAAAASSATISTDSESVSLGHRVRKDFRILHQEVNGSKLVYLDSA--------------A 80

Query: 871  CTELPCILPSRM*TSQNWSTWIMQQLHKSLLLLFWTLYKTITQPIIPMCIVVFIS*VQEP 692
             ++ P  +   +   QN+  +    +H+ +  L                         + 
Sbjct: 81   TSQKPAAVLDAL---QNYYEFYNSNVHRGIHYLS-----------------------AKA 114

Query: 691  LMNMNQRRIKVARFINASESTEIVFTRNATEAMNLVAYSWGLLNLRPGDEIILTIAEHHS 512
                   R KVARFINAS+S EIVFTRNATEA+NLVAYSWGL NL+PGDE+ILT+AEHHS
Sbjct: 115  TDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNLKPGDEVILTVAEHHS 174

Query: 511  AIVPFQIVAQKTGATLKFVSLTEEEEVPDIEKLKEMLSEKTKLVVTHHVSNTLGSVLPIK 332
             IVP+QIV+QKTGA LKFV+L E+E VPDI KL+E++S KTKLV  HHVSN L S LPI+
Sbjct: 175  CIVPWQIVSQKTGAVLKFVTLNEDE-VPDINKLRELISPKTKLVAVHHVSNVLASSLPIE 233

Query: 331  DITSWSHNVGAKVLVDACQSVPHMVVDVQNLDADFLVASSHKMCGPTGAGFLYGKSGILS 152
            +I  W+H+VGAKVLVDACQSVPHMVVDVQ L+ADFLVASSHKMCGPTG GFLYGKS +L 
Sbjct: 234  EIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMCGPTGIGFLYGKSDLLH 293

Query: 151  GMPPFLAGGEMISDVFLDYSTYAEPPSRFEAGTPAIGEVIGLGAAIDYLS 2
             MPPFL GGEMISDVFLD+STYAEPPSRFEAGTPAIGE I LGAA+DYLS
Sbjct: 294  SMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAVDYLS 343


>ref|XP_003546241.1| PREDICTED: cysteine desulfurase 2, chloroplastic-like [Glycine max]
          Length = 468

 Score =  389 bits (998), Expect = e-106
 Identities = 214/356 (60%), Positives = 248/356 (69%), Gaps = 2/356 (0%)
 Frame = -3

Query: 1063 FRKFS-CAATASSDIAVE-KSSLSLGHITRPDFPILHQNVNKSKLVYLDNXXXXXXXSGG 890
            FR+ S CA+T +  +A      LSLGH TRP FPILHQ VN SKLVYLDN          
Sbjct: 34   FRRVSVCASTVNEAVAEPIVGFLSLGHSTRPHFPILHQEVNGSKLVYLDNA--------- 84

Query: 889  ILVTIVCTELPCILPSRM*TSQNWSTWIMQQLHKSLLLLFWTLYKTITQPIIPMCIVVFI 710
                   ++ P  +   +   QN+       +H+ +  L                     
Sbjct: 85   -----ATSQKPTAVLKAL---QNYYEAYNSNVHRGIHFLS-------------------- 116

Query: 709  S*VQEPLMNMNQRRIKVARFINASESTEIVFTRNATEAMNLVAYSWGLLNLRPGDEIILT 530
                         R KVA FINA +S EIVFT+NA+EA+NLVAYSWGL NL+P DEIILT
Sbjct: 117  ---ARATDEYESARRKVASFINAIDSREIVFTKNASEAINLVAYSWGLSNLKPDDEIILT 173

Query: 529  IAEHHSAIVPFQIVAQKTGATLKFVSLTEEEEVPDIEKLKEMLSEKTKLVVTHHVSNTLG 350
            +AEHHSAIVP+QIVAQKTGA LKFV L ++E +PDI+KLKEMLS KTK+VV HHVSN L 
Sbjct: 174  VAEHHSAIVPWQIVAQKTGAVLKFVDLNQDE-IPDIDKLKEMLSRKTKIVVVHHVSNVLA 232

Query: 349  SVLPIKDITSWSHNVGAKVLVDACQSVPHMVVDVQNLDADFLVASSHKMCGPTGAGFLYG 170
            SVLPI+DI  W+H+VGAKVLVDACQSVPHM++DVQ+L+ DFLVASSHKMCGPTG GFLYG
Sbjct: 233  SVLPIQDIAQWAHDVGAKVLVDACQSVPHMMIDVQSLNVDFLVASSHKMCGPTGIGFLYG 292

Query: 169  KSGILSGMPPFLAGGEMISDVFLDYSTYAEPPSRFEAGTPAIGEVIGLGAAIDYLS 2
            K  +LS MPPFL GGEMISDV+LD+STYAEPPSRFEAGTPAIGE IGLGAAIDYLS
Sbjct: 293  KIDLLSSMPPFLGGGEMISDVYLDHSTYAEPPSRFEAGTPAIGEAIGLGAAIDYLS 348