BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00005218
         (445 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285453.1| PREDICTED: probable 3-beta-hydroxysteroid-De...   214   6e-54
gb|AFK46718.1| unknown [Lotus japonicus]                              212   2e-53
ref|XP_002304628.1| predicted protein [Populus trichocarpa] gi|2...   206   2e-51
gb|ADG02944.1| putative C-8,7 sterol isomerase [Gossypium hirsutum]   205   4e-51
ref|XP_003534175.1| PREDICTED: probable 3-beta-hydroxysteroid-De...   204   8e-51

>ref|XP_002285453.1| PREDICTED: probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
           isoform 2 [Vitis vinifera]
           gi|225435456|ref|XP_002285450.1| PREDICTED: probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
           isoform 1 [Vitis vinifera] gi|297746323|emb|CBI16379.3|
           unnamed protein product [Vitis vinifera]
          Length = 222

 Score =  214 bits (545), Expect = 6e-54
 Identities = 99/141 (70%), Positives = 118/141 (83%)
 Frame = -2

Query: 426 MEASNYSHPYVPRELVLPDFTPGFLSQSTILGVFAIASFLVITSSWIVSGRFPKLSKIER 247
           ME +  SHPYVP++L LP + P  LSQSTI+GV+ ++S LV++  WI+SGRFPK SKI+R
Sbjct: 1   MEGAPQSHPYVPKDLKLPGYVPVVLSQSTIVGVYGLSSLLVVSVIWIISGRFPKTSKIDR 60

Query: 246 SLMCWWAFTGLTHIILEGYFAFSPHFYKDKSAHFLAEVWKEYSKGDSRYAGRDSGVVSVE 67
            LMCWWAFTGLTH ILEGYF FSP FYKDK+A +LAEVWKEYSKGDSRYA RD+GV++VE
Sbjct: 61  LLMCWWAFTGLTHAILEGYFVFSPEFYKDKTACYLAEVWKEYSKGDSRYAARDAGVIAVE 120

Query: 66  GITAVIEGPACLLTVYAIAKK 4
           GITAV+EGPA LL +Y IA K
Sbjct: 121 GITAVLEGPASLLALYTIATK 141


>gb|AFK46718.1| unknown [Lotus japonicus]
          Length = 225

 Score =  212 bits (540), Expect = 2e-53
 Identities = 101/141 (71%), Positives = 118/141 (83%), Gaps = 2/141 (1%)
 Frame = -2

Query: 426 MEASN--YSHPYVPRELVLPDFTPGFLSQSTILGVFAIASFLVITSSWIVSGRFPKLSKI 253
           ME S   + HPYVPR+L LPD+ P FLSQS+IL VFAI++ LV++  WI SGR PK SK+
Sbjct: 1   MEGSQVAHRHPYVPRDLHLPDYVPCFLSQSSILSVFAISTLLVVSFIWIFSGRLPKKSKV 60

Query: 252 ERSLMCWWAFTGLTHIILEGYFAFSPHFYKDKSAHFLAEVWKEYSKGDSRYAGRDSGVVS 73
           +R LMCWWAFTGLTHIILEGYF F+P F+KDK+  +LAEVWKEYSKGDSRY GRD+GVV+
Sbjct: 61  DRVLMCWWAFTGLTHIILEGYFVFAPEFFKDKTGFYLAEVWKEYSKGDSRYVGRDAGVVT 120

Query: 72  VEGITAVIEGPACLLTVYAIA 10
           VEG+TAVIEGPA LL VYAIA
Sbjct: 121 VEGLTAVIEGPASLLAVYAIA 141


>ref|XP_002304628.1| predicted protein [Populus trichocarpa] gi|222842060|gb|EEE79607.1|
           predicted protein [Populus trichocarpa]
          Length = 220

 Score =  206 bits (523), Expect = 2e-51
 Identities = 93/132 (70%), Positives = 112/132 (84%)
 Frame = -2

Query: 405 HPYVPRELVLPDFTPGFLSQSTILGVFAIASFLVITSSWIVSGRFPKLSKIERSLMCWWA 226
           HPY PR+L LP + P FL+QSTI+GV+ + S LV++  WI+SGR  K++K+ R LMCWWA
Sbjct: 6   HPYAPRDLKLPGYVPNFLTQSTIVGVYLLTSLLVVSLIWILSGRSRKITKMNRLLMCWWA 65

Query: 225 FTGLTHIILEGYFAFSPHFYKDKSAHFLAEVWKEYSKGDSRYAGRDSGVVSVEGITAVIE 46
           FTGLTH+ILEGYFAFSP FYKDK+AH+LAEVWKEYSKGDSRYA RD+  V+VEG+TAV+E
Sbjct: 66  FTGLTHLILEGYFAFSPEFYKDKTAHYLAEVWKEYSKGDSRYAARDAATVTVEGLTAVLE 125

Query: 45  GPACLLTVYAIA 10
           GPA LL VYAIA
Sbjct: 126 GPASLLAVYAIA 137


>gb|ADG02944.1| putative C-8,7 sterol isomerase [Gossypium hirsutum]
          Length = 217

 Score =  205 bits (521), Expect = 4e-51
 Identities = 95/133 (71%), Positives = 112/133 (84%)
 Frame = -2

Query: 405 HPYVPRELVLPDFTPGFLSQSTILGVFAIASFLVITSSWIVSGRFPKLSKIERSLMCWWA 226
           HPYVPR+L LP + P  LSQSTIL V+ ++S LV++  W +SGR   +SK++R LMCWWA
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRSISKLDRLLMCWWA 62

Query: 225 FTGLTHIILEGYFAFSPHFYKDKSAHFLAEVWKEYSKGDSRYAGRDSGVVSVEGITAVIE 46
           FTGLTHIILEGYFAFSP FYKDK+  +LAEVWKEYSKGDSRYAGRDS +V+VEGIT+V+E
Sbjct: 63  FTGLTHIILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSTIVAVEGITSVLE 122

Query: 45  GPACLLTVYAIAK 7
           GP CLL VYAIAK
Sbjct: 123 GPPCLLAVYAIAK 135


>ref|XP_003534175.1| PREDICTED: probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase-like
           [Glycine max]
          Length = 219

 Score =  204 bits (518), Expect = 8e-51
 Identities = 97/133 (72%), Positives = 112/133 (84%)
 Frame = -2

Query: 408 SHPYVPRELVLPDFTPGFLSQSTILGVFAIASFLVITSSWIVSGRFPKLSKIERSLMCWW 229
           +HPYVPR+L LP + P FLS S IL VFA +S L++T  WI SGRF K +K++R LMCWW
Sbjct: 3   AHPYVPRDLHLPGYAPCFLSMSNILSVFASSSLLIVTLVWIFSGRFKK-TKVDRVLMCWW 61

Query: 228 AFTGLTHIILEGYFAFSPHFYKDKSAHFLAEVWKEYSKGDSRYAGRDSGVVSVEGITAVI 49
           AFTGLTHIILEGYF FSP F+KDK+  +LAEVWKEYSKGDSRYAGRD+GVV+VEGITAV+
Sbjct: 62  AFTGLTHIILEGYFVFSPEFFKDKTGFYLAEVWKEYSKGDSRYAGRDAGVVTVEGITAVL 121

Query: 48  EGPACLLTVYAIA 10
           EGPA LL VYAIA
Sbjct: 122 EGPASLLAVYAIA 134