BLASTX nr result
ID: Coptis21_contig00005166
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00005166
(1172 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004157624.1| PREDICTED: isoflavone 2'-hydroxylase-like [C... 68 1e-28
ref|XP_004140662.1| PREDICTED: isoflavone 2'-hydroxylase-like [C... 68 1e-28
gb|AEM42992.1| cytochrome P450 [Siraitia grosvenorii] 71 1e-26
emb|CBI33757.3| unnamed protein product [Vitis vinifera] 72 2e-26
ref|XP_002278841.2| PREDICTED: isoflavone 2'-hydroxylase [Vitis ... 72 3e-26
>ref|XP_004157624.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis sativus]
Length = 507
Score = 68.2 bits (165), Expect(4) = 1e-28
Identities = 32/64 (50%), Positives = 47/64 (73%)
Frame = -2
Query: 577 LICHRLTAIKECFSKNDIIFANRPSRLLGNEHLHYNYSTIASAQYGELWRNLRRLASTEI 398
++ ++AI+ECF+ NDI+FANRP + G ++L Y ST+ +A YG+ WRNLRRL++TEI
Sbjct: 76 VVVSSISAIEECFTTNDIVFANRPEFVSG-KYLTYGNSTLGAAPYGDHWRNLRRLSATEI 134
Query: 397 CQPI 386
I
Sbjct: 135 LSTI 138
Score = 56.6 bits (135), Expect(4) = 1e-28
Identities = 26/43 (60%), Positives = 33/43 (76%)
Frame = -3
Query: 693 IIGHLHLLKNPIHLAIANLIQKYGPILYLRFGFQPAVVLSSVT 565
I+GHLHLLK+PIH + NL QKYGPI LR G + VV+SS++
Sbjct: 40 ILGHLHLLKHPIHRTLHNLSQKYGPIFTLRLGSRLVVVVSSIS 82
Score = 46.2 bits (108), Expect(4) = 1e-28
Identities = 27/65 (41%), Positives = 44/65 (67%)
Frame = -1
Query: 401 NLSANIR*EEATIVI*EMYKGYDGDFKKVELKLIFTKLLFNMMARTLGMKPFFREEDMVD 222
N+SA IR EE I+I ++ + +F KV+LK +F++L FN++ R L K ++ EE + +
Sbjct: 141 NMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELTFNIIMRMLAGKRYYGEE-VSE 199
Query: 221 LEKAK 207
LE+AK
Sbjct: 200 LEEAK 204
Score = 22.7 bits (47), Expect(4) = 1e-28
Identities = 7/12 (58%), Positives = 9/12 (75%)
Frame = -3
Query: 147 FFPFLKWMDHSF 112
F PFLKW+D +
Sbjct: 223 FLPFLKWVDWQY 234
>ref|XP_004140662.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis sativus]
Length = 507
Score = 68.2 bits (165), Expect(4) = 1e-28
Identities = 32/64 (50%), Positives = 47/64 (73%)
Frame = -2
Query: 577 LICHRLTAIKECFSKNDIIFANRPSRLLGNEHLHYNYSTIASAQYGELWRNLRRLASTEI 398
++ ++AI+ECF+ NDI+FANRP + G ++L Y ST+ +A YG+ WRNLRRL++TEI
Sbjct: 76 VVVSSISAIEECFTTNDIVFANRPEFVSG-KYLTYGNSTLGAAPYGDHWRNLRRLSATEI 134
Query: 397 CQPI 386
I
Sbjct: 135 LSTI 138
Score = 56.6 bits (135), Expect(4) = 1e-28
Identities = 26/43 (60%), Positives = 33/43 (76%)
Frame = -3
Query: 693 IIGHLHLLKNPIHLAIANLIQKYGPILYLRFGFQPAVVLSSVT 565
I+GHLHLLK+PIH + NL QKYGPI LR G + VV+SS++
Sbjct: 40 ILGHLHLLKHPIHRTLHNLSQKYGPIFTLRLGSRLVVVVSSIS 82
Score = 46.2 bits (108), Expect(4) = 1e-28
Identities = 27/65 (41%), Positives = 44/65 (67%)
Frame = -1
Query: 401 NLSANIR*EEATIVI*EMYKGYDGDFKKVELKLIFTKLLFNMMARTLGMKPFFREEDMVD 222
N+SA IR EE I+I ++ + +F KV+LK +F++L FN++ R L K ++ EE + +
Sbjct: 141 NMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELTFNIIMRMLAGKRYYGEE-VSE 199
Query: 221 LEKAK 207
LE+AK
Sbjct: 200 LEEAK 204
Score = 22.7 bits (47), Expect(4) = 1e-28
Identities = 7/12 (58%), Positives = 9/12 (75%)
Frame = -3
Query: 147 FFPFLKWMDHSF 112
F PFLKW+D +
Sbjct: 223 FLPFLKWVDWQY 234
>gb|AEM42992.1| cytochrome P450 [Siraitia grosvenorii]
Length = 502
Score = 71.2 bits (173), Expect(3) = 1e-26
Identities = 35/60 (58%), Positives = 47/60 (78%)
Frame = -2
Query: 577 LICHRLTAIKECFSKNDIIFANRPSRLLGNEHLHYNYSTIASAQYGELWRNLRRLASTEI 398
+I L +ECF+KND+IFANRP RLL ++HL YN +T+A++ YG+ WRNLRRLA+ EI
Sbjct: 72 VIVSSLDIAEECFTKNDLIFANRP-RLLISKHLGYNCTTMATSPYGDHWRNLRRLAAIEI 130
Score = 51.2 bits (121), Expect(3) = 1e-26
Identities = 24/42 (57%), Positives = 30/42 (71%)
Frame = -3
Query: 693 IIGHLHLLKNPIHLAIANLIQKYGPILYLRFGFQPAVVLSSV 568
IIGHLHLLK PIH + +YGPI LRFG + AV++SS+
Sbjct: 36 IIGHLHLLKRPIHRNFHKIAAEYGPIFSLRFGSRLAVIVSSL 77
Score = 45.1 bits (105), Expect(3) = 1e-26
Identities = 24/75 (32%), Positives = 45/75 (60%), Gaps = 2/75 (2%)
Frame = -1
Query: 401 NLSANIR*EEATIVI*EMYKGYDGDFKKVELKLIFTKLLFNMMARTLGMKPFFREE--DM 228
N S +IR +E ++ +++ G G+F KVELK +F++L FN + R + K ++ +E D
Sbjct: 137 NSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRIVAGKRYYGDEVSDE 196
Query: 227 VDLEKAKDKMEELKI 183
+ + + MEE+ +
Sbjct: 197 EEAREFRGLMEEISL 211
>emb|CBI33757.3| unnamed protein product [Vitis vinifera]
Length = 1825
Score = 72.4 bits (176), Expect(4) = 2e-26
Identities = 33/54 (61%), Positives = 45/54 (83%)
Frame = -2
Query: 559 TAIKECFSKNDIIFANRPSRLLGNEHLHYNYSTIASAQYGELWRNLRRLASTEI 398
+A++ECF+KND+IFANRP RL+ +E+L Y Y++I S+ YGE WRNLRRL + EI
Sbjct: 83 SAVEECFTKNDVIFANRP-RLMVSEYLGYKYTSIVSSPYGEHWRNLRRLCALEI 135
Score = 53.5 bits (127), Expect(4) = 2e-26
Identities = 25/41 (60%), Positives = 30/41 (73%)
Frame = -3
Query: 693 IIGHLHLLKNPIHLAIANLIQKYGPILYLRFGFQPAVVLSS 571
I+GHLHLLK P H A+ +L + YGPI LRFG Q VV+SS
Sbjct: 41 ILGHLHLLKGPFHRALHHLSETYGPIFSLRFGSQLVVVISS 81
Score = 35.8 bits (81), Expect(4) = 2e-26
Identities = 22/72 (30%), Positives = 40/72 (55%)
Frame = -1
Query: 401 NLSANIR*EEATIVI*EMYKGYDGDFKKVELKLIFTKLLFNMMARTLGMKPFFREEDMVD 222
N+ IR +E ++ + + +F KVELK +F++L FN++ R + K ++ E D
Sbjct: 142 NMFLGIRKDEIKHLLRRLGRDSRDNFAKVELKSLFSELTFNIITRMVAGKRYYGEGS--D 199
Query: 221 LEKAKDKMEELK 186
E+AK E ++
Sbjct: 200 FEEAKHFREIIR 211
Score = 24.3 bits (51), Expect(4) = 2e-26
Identities = 12/33 (36%), Positives = 16/33 (48%), Gaps = 4/33 (12%)
Frame = -3
Query: 147 FFPFLKWMDHSFLVKLFIKR----DNLIQGWFD 61
F P L+WMD+ K K D ++QG D
Sbjct: 225 FLPILRWMDYGGYEKKMAKNSRELDVILQGLID 257
Score = 52.8 bits (125), Expect(2) = 5e-10
Identities = 22/41 (53%), Positives = 31/41 (75%)
Frame = -3
Query: 693 IIGHLHLLKNPIHLAIANLIQKYGPILYLRFGFQPAVVLSS 571
I GHLHLLK P+H + L +++GPI+ LRFG +P +V+SS
Sbjct: 579 IFGHLHLLKGPLHRTLHRLSERHGPIVSLRFGSRPVIVVSS 619
Score = 38.5 bits (88), Expect(2) = 5e-10
Identities = 14/23 (60%), Positives = 21/23 (91%)
Frame = -2
Query: 559 TAIKECFSKNDIIFANRPSRLLG 491
+A++ECF+KND+IFANRP ++G
Sbjct: 621 SAVEECFTKNDVIFANRPKFVMG 643
>ref|XP_002278841.2| PREDICTED: isoflavone 2'-hydroxylase [Vitis vinifera]
Length = 526
Score = 72.4 bits (176), Expect(4) = 3e-26
Identities = 33/54 (61%), Positives = 45/54 (83%)
Frame = -2
Query: 559 TAIKECFSKNDIIFANRPSRLLGNEHLHYNYSTIASAQYGELWRNLRRLASTEI 398
+A++ECF+KND+IFANRP RL+ +E+L Y Y++I S+ YGE WRNLRRL + EI
Sbjct: 101 SAVEECFTKNDVIFANRP-RLMVSEYLGYKYTSIVSSPYGEHWRNLRRLCALEI 153
Score = 53.5 bits (127), Expect(4) = 3e-26
Identities = 25/41 (60%), Positives = 30/41 (73%)
Frame = -3
Query: 693 IIGHLHLLKNPIHLAIANLIQKYGPILYLRFGFQPAVVLSS 571
I+GHLHLLK P H A+ +L + YGPI LRFG Q VV+SS
Sbjct: 59 ILGHLHLLKGPFHRALHHLSETYGPIFSLRFGSQLVVVISS 99
Score = 35.8 bits (81), Expect(4) = 3e-26
Identities = 22/72 (30%), Positives = 40/72 (55%)
Frame = -1
Query: 401 NLSANIR*EEATIVI*EMYKGYDGDFKKVELKLIFTKLLFNMMARTLGMKPFFREEDMVD 222
N+ IR +E ++ + + +F KVELK +F++L FN++ R + K ++ E D
Sbjct: 160 NMFLGIRKDEIKHLLRRLGRDSRDNFAKVELKSLFSELTFNIITRMVAGKRYYGEGS--D 217
Query: 221 LEKAKDKMEELK 186
E+AK E ++
Sbjct: 218 FEEAKHFREIIR 229
Score = 24.3 bits (51), Expect(4) = 3e-26
Identities = 12/33 (36%), Positives = 16/33 (48%), Gaps = 4/33 (12%)
Frame = -3
Query: 147 FFPFLKWMDHSFLVKLFIKR----DNLIQGWFD 61
F P L+WMD+ K K D ++QG D
Sbjct: 243 FLPILRWMDYGGYEKKMAKNSRELDVILQGLID 275