BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00004825
         (2455 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABI17894.1| vacuolar invertase [Coffea canephora]                  847   0.0  
gb|AAB47172.1| vacuolar invertase 2, GIN2 [Vitis vinifera=grape ...   839   0.0  
dbj|BAF34362.1| beta-fructofuranosidase [Citrus sinensis]             838   0.0  
dbj|BAB82419.1| acid invertase [Citrus unshiu]                        834   0.0  
sp|P80065.2|INVB_DAUCA RecName: Full=Beta-fructofuranosidase, so...   833   0.0  

>gb|ABI17894.1| vacuolar invertase [Coffea canephora]
          Length = 586

 Score =  847 bits (2187), Expect = 0.0
 Identities = 396/561 (70%), Positives = 464/561 (82%), Gaps = 1/561 (0%)
 Frame = +2

Query: 383  RGISEGVSEKSFRPLFGDAKSFPWSNTMLSWQRTGYHFQPEQNWINDPDGPLFYKGWYHL 562
            RG+ +GVSEK+FR +      +PW+N MLSWQRT YHFQPE+NW+NDP+GPLF+ GWYHL
Sbjct: 25   RGVPQGVSEKTFRGVSDANDVYPWTNAMLSWQRTSYHFQPEKNWMNDPNGPLFHMGWYHL 84

Query: 563  FYQYNPDSAVWGNITWAHAVSKDLMNWFHLPWAMVPDHWYDINGVWTGSATFLPGGSIVM 742
            FYQYNPDSA+WGNITW HAVS+DL++W +LP+AMVPD  +DINGVWTGSAT LPGG IV+
Sbjct: 85   FYQYNPDSAIWGNITWGHAVSRDLIHWLYLPFAMVPDRPFDINGVWTGSATILPGGQIVI 144

Query: 743  IYTGSTNESVQVQNVAFPANLSDPLLLNWVKYSGNPVLVPPPGIGVKDFRDPTTA-LYVG 919
            +YTG T + VQVQN+A+PANLSDPLLL+W+KY GNPV++PPPGIG KDFRDPTTA L   
Sbjct: 145  LYTGDTADLVQVQNLAYPANLSDPLLLDWIKYPGNPVMIPPPGIGKKDFRDPTTAWLAPD 204

Query: 920  GNKWRIVIGSKISTTGIALVYETADFLDYKLLDGLLHQVPTTGMWECIDLYPVSTTAKNG 1099
            G KW + +GSK++ TGIALVYET+DF  Y+LLDG+LH VP TGMWEC+D YPVSTT  NG
Sbjct: 205  GTKWLVTLGSKVNKTGIALVYETSDFKGYRLLDGVLHAVPRTGMWECVDFYPVSTTGDNG 264

Query: 1100 LDTSINGPEVKHVLKASLDDNKKDFYALGTYDVASNTWTPDDPELDVGIGIRYDYGKYYA 1279
            LDTS NGP  KHVLKASLD+NK D+YALGTYD  +N WTPDDPELDVGIG+R DYGKYYA
Sbjct: 265  LDTSANGPGTKHVLKASLDENKHDYYALGTYDPKNNKWTPDDPELDVGIGLRLDYGKYYA 324

Query: 1280 SKTFYDQNKQRRILWSWIGETDSENADILKEWSGLMGIPRVVTFDTKTKTNLLQWPVEEV 1459
            SKTFYDQNK+RRILW WIGETDSE AD++K W+ +  IPR V FD KT TN+LQWPVEE 
Sbjct: 325  SKTFYDQNKKRRILWGWIGETDSEAADLMKGWASVQTIPRTVVFDKKTGTNILQWPVEEA 384

Query: 1460 ESLRQSSKEFDRVELEAGSIVPLDVGTATQLDITADIEIDGQALERTIEADVLYNCSTSN 1639
            ESLR ++ EFD V+LE GSI PL++G+ATQLDI A  E+D +ALE T+EADV YNC+TS 
Sbjct: 385  ESLRFNATEFDTVKLEPGSIAPLNIGSATQLDIIASFEVDSEALEATVEADVGYNCTTSG 444

Query: 1640 GAAGRGALGPFGLLVFADESRSEQTAIYFYIAKGTDGHFKTFFCTDKSRSSKASDVGKQI 1819
            GAA RG LGPFGLLV AD S SE T +YFYI+K TDG  +T FC+D+SRSSKA DVGK +
Sbjct: 445  GAASRGKLGPFGLLVLADGSLSELTPVYFYISKSTDGSAETHFCSDESRSSKAPDVGKLV 504

Query: 1820 YGSIVPVLDGEKHTLRILVDHSITEAFAQGGRTCITSRVYPTEAIYGAARLFLFNNATQA 1999
            YGS VPVLDGEK + R+LVDHS+ E+FAQGGR  ITSRVYPT+AIYGAARLFLFNNAT  
Sbjct: 505  YGSTVPVLDGEKLSARLLVDHSVVESFAQGGRRVITSRVYPTKAIYGAARLFLFNNATGV 564

Query: 2000 NVTVTSLKIWQMSSASFQHYP 2062
            +VT  S KIW M SA  + +P
Sbjct: 565  SVT-ASAKIWHMRSADIRTFP 584


>gb|AAB47172.1| vacuolar invertase 2, GIN2 [Vitis vinifera=grape berries, Sultana,
            berries, Peptide, 664 aa]
          Length = 664

 Score =  839 bits (2167), Expect = 0.0
 Identities = 390/559 (69%), Positives = 461/559 (82%)
 Frame = +2

Query: 383  RGISEGVSEKSFRPLFGDAKSFPWSNTMLSWQRTGYHFQPEQNWINDPDGPLFYKGWYHL 562
            RG S GVSEKSFR   G    FPWSNTML+WQRTGYHFQPE+NW+NDP+GP+FY GWYH 
Sbjct: 101  RGPSTGVSEKSFRLHSGVRAPFPWSNTMLTWQRTGYHFQPEKNWMNDPNGPMFYGGWYHF 160

Query: 563  FYQYNPDSAVWGNITWAHAVSKDLMNWFHLPWAMVPDHWYDINGVWTGSATFLPGGSIVM 742
            FYQYNPD+AVWGNI W HAVSKDL+ W HLP AMV D WYD NGVWTGSAT L  G ++M
Sbjct: 161  FYQYNPDAAVWGNIVWGHAVSKDLIEWLHLPLAMVADQWYDTNGVWTGSATLLSDGQVIM 220

Query: 743  IYTGSTNESVQVQNVAFPANLSDPLLLNWVKYSGNPVLVPPPGIGVKDFRDPTTALYVGG 922
            +YTG+TNESVQVQN+A+PA+LSDPLL++WVKY GNPVLVPPPGI  KDFRDPTTA Y   
Sbjct: 221  LYTGATNESVQVQNLAYPADLSDPLLVDWVKYPGNPVLVPPPGIDDKDFRDPTTAWYWPD 280

Query: 923  NKWRIVIGSKISTTGIALVYETADFLDYKLLDGLLHQVPTTGMWECIDLYPVSTTAKNGL 1102
             KWRI IGSK++ TGI+LVY T DF  Y+L++G+LH VP TGMWEC+DLYPVS    NGL
Sbjct: 281  GKWRIAIGSKVNKTGISLVYNTEDFKKYELIEGVLHAVPGTGMWECVDLYPVSLKEDNGL 340

Query: 1103 DTSINGPEVKHVLKASLDDNKKDFYALGTYDVASNTWTPDDPELDVGIGIRYDYGKYYAS 1282
            DTS NGP VKHVLKASLDD+K D+YA+GTY + S  WTPD+P LDVGIG+RYDYGK+YAS
Sbjct: 341  DTSFNGPGVKHVLKASLDDDKNDYYAIGTYSLESGNWTPDNPNLDVGIGLRYDYGKFYAS 400

Query: 1283 KTFYDQNKQRRILWSWIGETDSENADILKEWSGLMGIPRVVTFDTKTKTNLLQWPVEEVE 1462
            KTFYDQNKQRRILW WIGETD E+ADI K W+ +  IPR V FD KT TN+LQWPV E++
Sbjct: 401  KTFYDQNKQRRILWGWIGETDGESADIKKGWASVQSIPRTVVFDKKTGTNILQWPVAEIK 460

Query: 1463 SLRQSSKEFDRVELEAGSIVPLDVGTATQLDITADIEIDGQALERTIEADVLYNCSTSNG 1642
            SLR+SSK+FD++E+  GS+V L+V  ATQ+DITA+ EID +AL+R  E+DV Y+C TS G
Sbjct: 461  SLRKSSKKFDKLEVGPGSVVTLEVEKATQMDITAEFEIDKEALKRIGESDVEYSCRTSGG 520

Query: 1643 AAGRGALGPFGLLVFADESRSEQTAIYFYIAKGTDGHFKTFFCTDKSRSSKASDVGKQIY 1822
            +A RG LGPFGLL+ ADE R EQT +YFY+AKGTDG  KTFFCTD+SRSS A+DV K+I+
Sbjct: 521  SAQRGELGPFGLLLLADEGRCEQTPVYFYVAKGTDGQLKTFFCTDESRSSLANDVDKRIF 580

Query: 1823 GSIVPVLDGEKHTLRILVDHSITEAFAQGGRTCITSRVYPTEAIYGAARLFLFNNATQAN 2002
            GS VPV+ GEK ++RILVDHSI E+FAQGGRTCITSRVYPT+AIYGAA+L++FNNAT A+
Sbjct: 581  GSTVPVIKGEKLSMRILVDHSIIESFAQGGRTCITSRVYPTKAIYGAAQLYVFNNATSAS 640

Query: 2003 VTVTSLKIWQMSSASFQHY 2059
            +T  S++ W M SA  + Y
Sbjct: 641  IT-ASIQTWAMKSAYIRPY 658


>dbj|BAF34362.1| beta-fructofuranosidase [Citrus sinensis]
          Length = 687

 Score =  838 bits (2166), Expect = 0.0
 Identities = 389/565 (68%), Positives = 470/565 (83%)
 Frame = +2

Query: 368  RPYTPRGISEGVSEKSFRPLFGDAKSFPWSNTMLSWQRTGYHFQPEQNWINDPDGPLFYK 547
            RP+T    S GVSEKS R       S+PW+N MLSWQRT +HFQP++NW+NDP+GP+FYK
Sbjct: 83   RPFTSAS-SAGVSEKSNRRFGARTASYPWTNNMLSWQRTAFHFQPQKNWMNDPNGPVFYK 141

Query: 548  GWYHLFYQYNPDSAVWGNITWAHAVSKDLMNWFHLPWAMVPDHWYDINGVWTGSATFLPG 727
            GWYHLFYQYNP+ A+WG+I W HAVSKDL++W+HLP AMV D WYDI GVWTGSAT LP 
Sbjct: 142  GWYHLFYQYNPNGAIWGDIVWGHAVSKDLIHWYHLPLAMVADQWYDIMGVWTGSATILPD 201

Query: 728  GSIVMIYTGSTNESVQVQNVAFPANLSDPLLLNWVKYSGNPVLVPPPGIGVKDFRDPTTA 907
            G ++M+YTGSTNESVQVQN+A+PA+ SDPLL+ WVKY GNPVLVPPPGIG KDFRDPTTA
Sbjct: 202  GKLMMLYTGSTNESVQVQNLAYPADPSDPLLIKWVKYPGNPVLVPPPGIGAKDFRDPTTA 261

Query: 908  LYVGGNKWRIVIGSKISTTGIALVYETADFLDYKLLDGLLHQVPTTGMWECIDLYPVSTT 1087
                  KWRI IGS+I+ TGI  VY+T DF++Y+LL G+LH VP TGMWEC+D YPVSTT
Sbjct: 262  WLTSEGKWRIAIGSRINRTGITFVYDTKDFINYELLRGVLHGVPNTGMWECVDFYPVSTT 321

Query: 1088 AKNGLDTSINGPEVKHVLKASLDDNKKDFYALGTYDVASNTWTPDDPELDVGIGIRYDYG 1267
             ++GLDTS NGP VKHV+KAS+DD++ D+YA+GTY   + TW PD+PE+DVGIGIRYDYG
Sbjct: 322  GEHGLDTSHNGPGVKHVVKASMDDDRHDYYAIGTYHEKNVTWVPDNPEIDVGIGIRYDYG 381

Query: 1268 KYYASKTFYDQNKQRRILWSWIGETDSENADILKEWSGLMGIPRVVTFDTKTKTNLLQWP 1447
             +YASKTFYDQNK+RR+LW WIGE+DSE AD+ K W+ L GIPR V  DTKT +NLLQWP
Sbjct: 382  LFYASKTFYDQNKKRRVLWGWIGESDSEIADVKKGWASLQGIPRTVALDTKTGSNLLQWP 441

Query: 1448 VEEVESLRQSSKEFDRVELEAGSIVPLDVGTATQLDITADIEIDGQALERTIEADVLYNC 1627
            VEEV+SLR +SKEF ++EL+ GS++PLDVG+ATQLDI A+ E+D  ALE+T E++V ++C
Sbjct: 442  VEEVDSLRLTSKEFKKIELKPGSVMPLDVGSATQLDIVAEFELDKAALEKTAESNVEFSC 501

Query: 1628 STSNGAAGRGALGPFGLLVFADESRSEQTAIYFYIAKGTDGHFKTFFCTDKSRSSKASDV 1807
            S+S G+A RGALGPFGLLV AD+S SEQT +YFYIAKG DG  KT+FCTD+SRSS+A+DV
Sbjct: 502  SSSEGSAERGALGPFGLLVLADDSLSEQTPVYFYIAKGKDGSLKTYFCTDQSRSSEANDV 561

Query: 1808 GKQIYGSIVPVLDGEKHTLRILVDHSITEAFAQGGRTCITSRVYPTEAIYGAARLFLFNN 1987
             K  YGS VPVL+GEK ++R+LVDHSI E FAQGGRT ITSRVYPT+AIYGAAR+FLFNN
Sbjct: 562  NKSKYGSFVPVLEGEKFSMRVLVDHSIVEGFAQGGRTTITSRVYPTKAIYGAARVFLFNN 621

Query: 1988 ATQANVTVTSLKIWQMSSASFQHYP 2062
            AT A+VT +SLK WQM+SA  + YP
Sbjct: 622  ATGASVT-SSLKAWQMNSAFIRPYP 645


>dbj|BAB82419.1| acid invertase [Citrus unshiu]
          Length = 687

 Score =  834 bits (2154), Expect = 0.0
 Identities = 388/565 (68%), Positives = 469/565 (83%)
 Frame = +2

Query: 368  RPYTPRGISEGVSEKSFRPLFGDAKSFPWSNTMLSWQRTGYHFQPEQNWINDPDGPLFYK 547
            RP+T    S GVSEKS R       S+PW+N MLSWQRT +HFQP++NW+NDP+GP+FYK
Sbjct: 83   RPFTSAS-SAGVSEKSNRRFGSRTASYPWTNNMLSWQRTAFHFQPQKNWMNDPNGPVFYK 141

Query: 548  GWYHLFYQYNPDSAVWGNITWAHAVSKDLMNWFHLPWAMVPDHWYDINGVWTGSATFLPG 727
            GWYHLFYQYNP+ A+WG+I W HAVSKDL++W+HLP AMV D WYDI GVWTGSAT LP 
Sbjct: 142  GWYHLFYQYNPNGAIWGDIVWGHAVSKDLIHWYHLPLAMVADQWYDIMGVWTGSATILPD 201

Query: 728  GSIVMIYTGSTNESVQVQNVAFPANLSDPLLLNWVKYSGNPVLVPPPGIGVKDFRDPTTA 907
            G ++M+YTGSTNESVQVQN+A+PA+ SDPLL+ WVKY GNPVLVPPPGIG KDFRDPTTA
Sbjct: 202  GKLMMLYTGSTNESVQVQNLAYPADPSDPLLIKWVKYPGNPVLVPPPGIGSKDFRDPTTA 261

Query: 908  LYVGGNKWRIVIGSKISTTGIALVYETADFLDYKLLDGLLHQVPTTGMWECIDLYPVSTT 1087
                  KWRI IGS+I+ TGI  VY+T DF++Y+LL G+LH VP TGMWEC+D YPVSTT
Sbjct: 262  WLTSEGKWRIAIGSRINRTGITFVYDTKDFINYELLRGVLHGVPNTGMWECVDFYPVSTT 321

Query: 1088 AKNGLDTSINGPEVKHVLKASLDDNKKDFYALGTYDVASNTWTPDDPELDVGIGIRYDYG 1267
             ++GLDTS NG  VKHV+KAS+DD++ D+YA+GTY   + TW PD+PE+DVGIGIRYDYG
Sbjct: 322  GEHGLDTSHNGLGVKHVVKASMDDDRHDYYAIGTYHEKNVTWVPDNPEIDVGIGIRYDYG 381

Query: 1268 KYYASKTFYDQNKQRRILWSWIGETDSENADILKEWSGLMGIPRVVTFDTKTKTNLLQWP 1447
             +YASKTFYDQNK+RR+LW WIGE+DSE AD+ K W+ L GIPR V  DTKT +NLLQWP
Sbjct: 382  LFYASKTFYDQNKKRRVLWGWIGESDSEIADVKKGWASLQGIPRTVALDTKTGSNLLQWP 441

Query: 1448 VEEVESLRQSSKEFDRVELEAGSIVPLDVGTATQLDITADIEIDGQALERTIEADVLYNC 1627
            VEEV+SLR +SKEF ++EL+ GS++PLDVG+ATQLDI A+ E+D  ALE+T E++V ++C
Sbjct: 442  VEEVDSLRLTSKEFKKIELKPGSVMPLDVGSATQLDIVAEFELDKAALEKTAESNVEFSC 501

Query: 1628 STSNGAAGRGALGPFGLLVFADESRSEQTAIYFYIAKGTDGHFKTFFCTDKSRSSKASDV 1807
            S+S G+A RGALGPFGLLV AD+S SEQT +YFYIAKG DG  KT+FCTD+SRSS+A+DV
Sbjct: 502  SSSEGSAERGALGPFGLLVLADDSLSEQTPVYFYIAKGKDGSLKTYFCTDQSRSSEANDV 561

Query: 1808 GKQIYGSIVPVLDGEKHTLRILVDHSITEAFAQGGRTCITSRVYPTEAIYGAARLFLFNN 1987
             K  YGS VPVL+GEK ++R+LVDHSI E FAQGGRT ITSRVYPT+AIYGAAR+FLFNN
Sbjct: 562  NKSKYGSFVPVLEGEKFSMRVLVDHSIVEGFAQGGRTTITSRVYPTKAIYGAARVFLFNN 621

Query: 1988 ATQANVTVTSLKIWQMSSASFQHYP 2062
            AT A+VT +SLK WQM+SA  + YP
Sbjct: 622  ATGASVT-SSLKAWQMNSAFIRPYP 645


>sp|P80065.2|INVB_DAUCA RecName: Full=Beta-fructofuranosidase, soluble isoenzyme I; AltName:
            Full=Invertase; AltName: Full=Saccharase; AltName:
            Full=Sucrose hydrolase; Flags: Precursor
            gi|407055|emb|CAA53097.1| beta-fructofuranosidase [Daucus
            carota] gi|4454117|emb|CAA77267.1|
            beta-fructofuranosidase, isoform I [Daucus carota]
          Length = 661

 Score =  833 bits (2151), Expect = 0.0
 Identities = 399/659 (60%), Positives = 488/659 (74%), Gaps = 8/659 (1%)
 Frame = +2

Query: 110  VNPFSSFPDLENEYPSSYTPLPYHVVQQQETDQKKSHTPKKGIXXXXXXXXXXXXXXXXI 289
            ++ +   P  + E+ SSYTP P     + E D  +S T ++ I                I
Sbjct: 1    MDTYHFLPSRDLEHASSYTPRPDSPETRHEPDPDRSKTNRRPIKIVSSVLLSTLILSFVI 60

Query: 290  ----GPDQRSVGPIVIXXXXXXXXXXXXXXRPYT----PRGISEGVSEKSFRPLFGDAKS 445
                 P+ + V    +               P       RG+S+GVSEKSFR    +  S
Sbjct: 61   FLLVNPNVQQVVRKKVSKNSNGEDRNKASKSPEMLGPPSRGVSQGVSEKSFRQATAEP-S 119

Query: 446  FPWSNTMLSWQRTGYHFQPEQNWINDPDGPLFYKGWYHLFYQYNPDSAVWGNITWAHAVS 625
            +PW+N MLSWQRT +HFQP++NW+NDP+GPLF+ GWYHLFYQYNPDSA+WGNITW HA+S
Sbjct: 120  YPWTNDMLSWQRTSFHFQPQENWMNDPNGPLFHMGWYHLFYQYNPDSAIWGNITWGHAIS 179

Query: 626  KDLMNWFHLPWAMVPDHWYDINGVWTGSATFLPGGSIVMIYTGSTNESVQVQNVAFPANL 805
            +DL+NW HLP+AM PD WYDINGVWTGSAT LP G IVM+YTG T++ VQVQN+A+PANL
Sbjct: 180  RDLINWLHLPFAMQPDQWYDINGVWTGSATVLPDGKIVMLYTGDTDDLVQVQNLAYPANL 239

Query: 806  SDPLLLNWVKYSGNPVLVPPPGIGVKDFRDPTTALYVGGNKWRIVIGSKISTTGIALVYE 985
            SDPLLL+W+KY  NPV+ PPPGIG  DFRDPTTA      KWRI IGSK++ TGI+L+Y+
Sbjct: 240  SDPLLLDWIKYPDNPVMFPPPGIGSTDFRDPTTAWIGRDGKWRITIGSKVNKTGISLMYK 299

Query: 986  TADFLDYKLLDGLLHQVPTTGMWECIDLYPVSTTAKNGLDTSINGPEVKHVLKASLDDNK 1165
            T DF+ Y+LLD LLH VP TGMWEC+D YPVS T  NGLDTS+NGP VKHVLK+SLDD++
Sbjct: 300  TTDFITYELLDNLLHAVPGTGMWECVDFYPVSVTGSNGLDTSVNGPGVKHVLKSSLDDDR 359

Query: 1166 KDFYALGTYDVASNTWTPDDPELDVGIGIRYDYGKYYASKTFYDQNKQRRILWSWIGETD 1345
             D+YALGTYD  ++ WTPD+PELDVGIG+R DYGKYYASKTFYDQ+K+RR+LW WIGETD
Sbjct: 360  HDYYALGTYDPINDKWTPDNPELDVGIGLRLDYGKYYASKTFYDQDKERRLLWGWIGETD 419

Query: 1346 SENADILKEWSGLMGIPRVVTFDTKTKTNLLQWPVEEVESLRQSSKEFDRVELEAGSIVP 1525
            SE+AD+LK W+ +  IPR V FD KT TN+LQWPV+EVESLR  S E D VEL+ GS+VP
Sbjct: 420  SESADLLKGWASVQSIPRTVVFDKKTGTNILQWPVKEVESLRSRSYEIDDVELKPGSLVP 479

Query: 1526 LDVGTATQLDITADIEIDGQALERTIEADVLYNCSTSNGAAGRGALGPFGLLVFADESRS 1705
            L + +A QLDI A  E+D +A + T EAD  YNC+ S GAAGRG LGPFG+LV AD+  S
Sbjct: 480  LKISSAAQLDIVASFEVDEEAFKGTYEADASYNCTASEGAAGRGILGPFGILVLADDPLS 539

Query: 1706 EQTAIYFYIAKGTDGHFKTFFCTDKSRSSKASDVGKQIYGSIVPVLDGEKHTLRILVDHS 1885
            E T +YFYIAKG DG+ KT+FC D+SRSS ASDV K++YGS VPVL GE  ++R+LVDHS
Sbjct: 540  ELTPVYFYIAKGVDGNAKTYFCADQSRSSTASDVDKEVYGSDVPVLHGESLSMRLLVDHS 599

Query: 1886 ITEAFAQGGRTCITSRVYPTEAIYGAARLFLFNNATQANVTVTSLKIWQMSSASFQHYP 2062
            I E+FAQGGRT ITSRVYPT AIY AAR+FLFNNAT  +VT  S+K WQM+SA+ + +P
Sbjct: 600  IVESFAQGGRTVITSRVYPTRAIYSAARVFLFNNATGVSVT-ASVKAWQMASATLKPFP 657