BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00004329
         (1365 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransf...   679   0.0  
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]        260   7e-67
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...   238   2e-60
ref|XP_002277927.2| PREDICTED: 8-hydroxyquercetin 8-O-methyltran...   238   2e-60
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...   238   4e-60

>sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransferase; Short=CoOMT; AltName:
            Full=Tetrahydrocolumbamine 2-O-methyltransferase
            gi|24210466|dbj|BAC22084.1| columbamine
            O-methyltransferase [Coptis japonica]
          Length = 351

 Score =  679 bits (1751), Expect = 0.0
 Identities = 340/351 (96%), Positives = 340/351 (96%)
 Frame = +3

Query: 60   MDTPNTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGPVTLSQLVTHLP 239
            MDTPNTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGPVTLSQLVTHLP
Sbjct: 1    MDTPNTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGPVTLSQLVTHLP 60

Query: 240  LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQT 419
            LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQT
Sbjct: 61   LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQT 120

Query: 420  HPEEFSVWSHVINVLDGKKPYWESNDTSMYEKTEGDPEINEILNDAMTSHSTFMLPALVS 599
            HPEEFSVWSHVINVLDGKKPYWESNDTSMYEKTEGDPEINEILNDAMTSHSTFMLPALVS
Sbjct: 121  HPEEFSVWSHVINVLDGKKPYWESNDTSMYEKTEGDPEINEILNDAMTSHSTFMLPALVS 180

Query: 600  GLMKENVLDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAG 779
            GLMKENVLDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAG
Sbjct: 181  GLMKENVLDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAG 240

Query: 780  DMFTSIPNADAILLKSTLHNYEDDDCIKILNIAKEALPSTGGKVILVEIVVDTENLPLFT 959
            DMFTSIPNADAILLKSTLHNYEDDDCIKILNIAKEALPSTGGKVILVEIVVDTENLPLFT
Sbjct: 241  DMFTSIPNADAILLKSTLHNYEDDDCIKILNIAKEALPSTGGKVILVEIVVDTENLPLFT 300

Query: 960  SARXXXXXXXXXXXGKERTKKEWEDLLRKANFTSHQVIPIMAIESIIVAYS 1112
            SAR           GKERTKKEWEDLLRKANFTSHQVIPIMAIESIIVAYS
Sbjct: 301  SARLSMGMDMMLMSGKERTKKEWEDLLRKANFTSHQVIPIMAIESIIVAYS 351


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score =  260 bits (664), Expect = 7e-67
 Identities = 133/338 (39%), Positives = 211/338 (62%), Gaps = 3/338 (0%)
 Frame = +3

Query: 105  QAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNG-PVTLSQLVTHLPLKSTSIDRFHHFMR 281
            QA++WKH++GFAE+++L+S V L I D IHN+G P+TLS+L + LP++   +DR +  MR
Sbjct: 14   QAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPVQPVDLDRLYRVMR 73

Query: 282  YLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQTHPEEFSVWSHVIN- 458
            YLVHM+LF    D+  +  KY L P +K L+ G +KS+   ++L    +  S W H+   
Sbjct: 74   YLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILLINDKDFMSPWHHLKEG 133

Query: 459  VLDGKKPYWESNDTSMYEKTEGDPEINEILNDAMTSHSTFMLPALVSGLMKENVLDGVAS 638
            +++    + ++   S+++    +P+ N++ N+ M   S  +  ALV+     N+  G+ +
Sbjct: 134  LVNNSNAFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALVNEC--GNIFQGIQT 191

Query: 639  IVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAGDMFTSIPNADAIL 818
            +VDVGG +G   K +  AFPH+KCS+ DL HVI      P +  + G+MF SIP+ADAIL
Sbjct: 192  LVDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGEMFKSIPSADAIL 251

Query: 819  LKSTLHNYEDDDCIKILNIAKEALPSTGGKVILVEIVVDTENLPLFTSAR-XXXXXXXXX 995
            +K  LH++ DD+CI+IL   KEA+P  GGKVI+V+IV+D E+   ++  R          
Sbjct: 252  MKCILHDWNDDECIEILKKCKEAVPKEGGKVIIVDIVLDMESKHPYSKVRLTLDLDMMLN 311

Query: 996  XXGKERTKKEWEDLLRKANFTSHQVIPIMAIESIIVAY 1109
              GKERT++EW+ L+  A F S+++  I A++S+I A+
Sbjct: 312  TEGKERTEEEWKKLIDGAGFASYKITQISALQSVIEAF 349


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
            glaucum]
          Length = 350

 Score =  238 bits (608), Expect = 2e-60
 Identities = 125/348 (35%), Positives = 212/348 (60%), Gaps = 6/348 (1%)
 Frame = +3

Query: 84   NDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNG-PVTLSQLVTHLPLKSTSID 260
            N + + +QA++W  ++GFA++++L+S V L + +IIHN+G P+TLS+L  HLP +  + D
Sbjct: 5    NKENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQD 64

Query: 261  RFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQTHPEEFSV 440
              +  +RYLVHM+LFT S+  I  E +Y L P +K LV G  K +   ++  T  +  + 
Sbjct: 65   ALYRVLRYLVHMKLFTKSS--IDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAP 122

Query: 441  WSH----VINVLDGKKPYWESNDTSMYEKTEGDPEINEILNDAMTSHSTFMLPALVSGLM 608
            W +    ++N       + ++  T++++     PE N++ N+ M + +  ++ ALV    
Sbjct: 123  WHYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKEC- 181

Query: 609  KENVLDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAGDMF 788
              ++ DG+ +IVDVGG +G   + I  AFPH+KC+V DL HVI       +++ + GDMF
Sbjct: 182  -SSMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMF 240

Query: 789  TSIPNADAILLKSTLHNYEDDDCIKILNIAKEALPSTGGKVILVEIVVDTENLPLFTSAR 968
              IPNADAI++K  LH+++D +CI+IL   K+A+P  GGKVI+++I++D ++   +T  R
Sbjct: 241  KYIPNADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEHPYTKMR 300

Query: 969  -XXXXXXXXXXXGKERTKKEWEDLLRKANFTSHQVIPIMAIESIIVAY 1109
                        GKERT++EW+ L+  A +  +++  I A++S+I AY
Sbjct: 301  LTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAY 348


>ref|XP_002277927.2| PREDICTED: 8-hydroxyquercetin 8-O-methyltransferase isoform 2 [Vitis
            vinifera]
          Length = 357

 Score =  238 bits (608), Expect = 2e-60
 Identities = 132/358 (36%), Positives = 209/358 (58%), Gaps = 8/358 (2%)
 Frame = +3

Query: 60   MDTPNTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNG-PVTLSQLVTHL 236
            MD  N+  + + ++AQA VW H+F F  ++ L+  + LGIPDIIHN+G P+TL QLV  L
Sbjct: 1    MDPANSEMSSELLQAQAHVWNHIFNFVNSMSLKCAIQLGIPDIIHNHGKPMTLPQLVAKL 60

Query: 237  PLKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDK-YELTPASKLLVHGHQKSLAPYVML 413
            P+        +  MR LV    F     Q ++E++ Y LT AS+LL+     S+ P+++ 
Sbjct: 61   PVHPKKTQYVYRLMRVLVQSDFFAAQRAQQSEEEEGYVLTHASRLLLKDDSLSVRPFLLA 120

Query: 414  QTHPEEFSVWSHVINVL--DGKKPYWESNDTSMYEKTEGDPEINEILNDAMTSHSTFMLP 587
               P     W +V      D   P+  +++ ++++    +P++N   N+AM S +  +  
Sbjct: 121  MLDPTLTKPWHYVSAWFQNDDPTPFDTAHERTLWDYAGHEPQLNYFFNEAMASDARLVTS 180

Query: 588  ALVSGLMKENVLDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLD 767
             LV     + V +G+ S+VDVGG +G VAK I +AFPH+KC+V+DL HV+  +  +  L+
Sbjct: 181  VLVKE--GKGVFEGLNSLVDVGGGTGTVAKAIANAFPHLKCTVLDLPHVVAGLQGSKNLN 238

Query: 768  YVAGDMFTSIPNADAILLKSTLHNYEDDDCIKILNIAKEALPS--TGGKVILVEIVVDTE 941
            Y AGDMF +IP ADAILLK  LH++ D++C+KIL   +EA+PS   GGKVI+++++V  +
Sbjct: 239  YFAGDMFEAIPPADAILLKWILHDWSDEECVKILKRCREAIPSKERGGKVIIIDMMVKNQ 298

Query: 942  --NLPLFTSARXXXXXXXXXXXGKERTKKEWEDLLRKANFTSHQVIPIMAIESIIVAY 1109
              +     +             GKER +KEWE L  +A F+ +++ PI+ + S+I  Y
Sbjct: 299  KGDCKSRETQLFFDMLMMVLVTGKEREEKEWEKLFLEAGFSHYKITPILGLRSLIEVY 356


>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase; Short=6-OMT
            gi|9711914|dbj|BAB08004.1|
            S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score =  238 bits (606), Expect = 4e-60
 Identities = 129/344 (37%), Positives = 211/344 (61%), Gaps = 4/344 (1%)
 Frame = +3

Query: 90   DEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGP-VTLSQLVTHLPLKSTSIDRF 266
            D + +QA++W  ++GFAE+++L+  V L + +IIHN+G  +TLS+L + LP +  + D  
Sbjct: 6    DNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNEDAL 65

Query: 267  HHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQTHPEEFSVWS 446
            +  MRYLVHM+LFT ++  I  E +Y L P +K LV G  K +   ++  T  +  + W 
Sbjct: 66   YRVMRYLVHMKLFTKAS--IDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPWH 123

Query: 447  HVINVLDGKK--PYWESNDTSMYEKTEGDPEINEILNDAMTSHSTFMLPALVSGLMKENV 620
            ++ + L G+    + ++  T+++      PE N++ N+AM + S  ++ ALV      N+
Sbjct: 124  YLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKEC--GNI 181

Query: 621  LDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAGDMFTSIP 800
             +G+ ++VDVGG +G   + I +AFPH+KC+V DL HVI       ++  VAGDMF  IP
Sbjct: 182  FNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIP 241

Query: 801  NADAILLKSTLHNYEDDDCIKILNIAKEALPSTGGKVILVEIVVDTENLPLFTSAR-XXX 977
             ADAI++K  LH+++D +CI+IL   KEA+P  GGKVI+V+IV++ ++   +T  R    
Sbjct: 242  KADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLD 301

Query: 978  XXXXXXXXGKERTKKEWEDLLRKANFTSHQVIPIMAIESIIVAY 1109
                    GKERT++EW+ L+  A +  H++  I A++S+I AY
Sbjct: 302  LDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAY 345