BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00004189
         (1998 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   509   e-142
ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   469   e-129
ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|2...   460   e-127
ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabid...   422   e-115
ref|XP_003553411.1| PREDICTED: LOW QUALITY PROTEIN: cytoplasmic ...   420   e-115

>ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
            gi|297739656|emb|CBI29838.3| unnamed protein product
            [Vitis vinifera]
          Length = 468

 Score =  509 bits (1312), Expect = e-142
 Identities = 271/433 (62%), Positives = 325/433 (75%), Gaps = 16/433 (3%)
 Frame = -2

Query: 1931 DNQIENTPRTCYKCKVAKFIESTGGL---------CGECFDRSLYGKFKQAVTTQNLISP 1779
            D  + N    C KCKV + + S+G +         C +CF  +L+GKFK AVT+  +ISP
Sbjct: 37   DIDLPNHHNLCLKCKVNETLASSGTVSVDGDIGRFCFDCFRGNLFGKFKLAVTSNAMISP 96

Query: 1778 TDNVLLAFSGGPSSRVALEFVHEMQNKAQKNRDASRDKSYPVFGVGVAFIDESAVTSL-- 1605
            TDNVL+AFSGGPSSRVAL+FVHEMQ+KAQKN DASRD+S PVFGVGVAF+DESAV+ +  
Sbjct: 97   TDNVLVAFSGGPSSRVALQFVHEMQHKAQKNFDASRDRSLPVFGVGVAFVDESAVSRIPS 156

Query: 1604 -QLDKAIQDLRMIVSTLAPPRKELHIMPIQTILSHDDDAVNRLKELLLTVTDVTGKEDLL 1428
             + DKAIQD++ IVS LAPP K  H++PI+++ S   +  +RL+ELL  V DVTGKEDLL
Sbjct: 157  DEFDKAIQDMKSIVSNLAPPDKAFHVVPIESVYS--SNGRDRLEELLNAVKDVTGKEDLL 214

Query: 1427 LHLRMLCLQKVALENNYNKLILGSCTSRIACHVLSATVKGQGYSLPADIQYADARWEIPV 1248
             HLRML LQK A EN Y KL+LGSCTSRIACHV+SATVKGQGYSLPADIQY DARWEIPV
Sbjct: 215  QHLRMLSLQKNAYENGYTKLLLGSCTSRIACHVISATVKGQGYSLPADIQYVDARWEIPV 274

Query: 1247 VLPLRDCVSQELNMLCRLNSLKTLDSFDSPRSGINELVSTFITLLTEENPARERTIVRTA 1068
            VLPLRDC++QELNMLCRL+ LKT++  +SP SGIN LV++F+TLL EENP+RE TIVRTA
Sbjct: 275  VLPLRDCLAQELNMLCRLDGLKTMEVLNSPHSGINGLVASFVTLLQEENPSRECTIVRTA 334

Query: 1067 EKLVPFQFNRINESLDS----GDRRRRRKVNLKSYESISSEFLCPICCSPVNNLDLEYLK 900
             KL PF FN+I ES DS      RRR++K NLK  ES+  E  CPIC SP++  D   L 
Sbjct: 335  GKLTPFHFNQIPESDDSNVHLASRRRQKKFNLKPNESVPPESYCPICNSPLSKSDHINL- 393

Query: 899  SKLESCATSPTVFGDSCCPSCQFQILPKESSSMEHFYSLVPQQMITRVKDSVGGDRAWLR 720
            S L S  T+  +FG +CCPSCQFQILPKE  SMEHFYSL+PQQ + + KD     +  LR
Sbjct: 394  SSLGSDQTNADIFGATCCPSCQFQILPKEPVSMEHFYSLLPQQFVVQAKDRSFSMQRQLR 453

Query: 719  EQIEDCLLSDGED 681
            EQI+D LLSD ED
Sbjct: 454  EQIQDFLLSDSED 466


>ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Glycine max]
          Length = 458

 Score =  469 bits (1206), Expect = e-129
 Identities = 246/434 (56%), Positives = 320/434 (73%), Gaps = 13/434 (2%)
 Frame = -2

Query: 1940 EEVDNQIENTPRTCYKCKVAKFIESTGGL-----CGECFDRSLYGKFKQAVTTQNLISPT 1776
            EE D++  N    C KCK+   +   GG+     C +CF  +L+GKF+ AVT+  +I+PT
Sbjct: 28   EEADSK--NPSNVCIKCKLNDAVSGYGGIDDGRFCADCFKTNLFGKFRFAVTSNAMITPT 85

Query: 1775 DNVLLAFSGGPSSRVALEFVHEMQNKAQKNRDASRDKSYPVFGVGVAFIDESAVTSL--- 1605
            D VL+AFSGGPSSRVAL+FVH+MQ +AQ+N DASRD+S PVFGVGV FIDESAV  +   
Sbjct: 86   DKVLVAFSGGPSSRVALQFVHDMQERAQRNFDASRDRSLPVFGVGVVFIDESAVLPIPSN 145

Query: 1604 QLDKAIQDLRMIVSTLAPPRKELHIMPIQTILSHDD-DAVNRLKELLLTVTDVTGKEDLL 1428
            ++++A++ +  +VS+LAPPRKELHI+PI+T+ S D  D   R+ +++ TV D TG+ED+L
Sbjct: 146  EMEEAVEVVSEVVSSLAPPRKELHIVPIETVYSSDSGDGKERITKVVNTVIDPTGREDVL 205

Query: 1427 LHLRMLCLQKVALENNYNKLILGSCTSRIACHVLSATVKGQGYSLPADIQYADARWEIPV 1248
            L LRML LQKVA E  YN++ILGSC SRIACHV+SATVKGQGYSLPADIQY DARWE+PV
Sbjct: 206  LCLRMLALQKVASEFGYNRIILGSCISRIACHVISATVKGQGYSLPADIQYVDARWEVPV 265

Query: 1247 VLPLRDCVSQELNMLCRLNSLKTLDSFDSPRSGINELVSTFITLLTEENPARERTIVRTA 1068
            VLPLRDC +QE+NMLC L+ LKT+       S IN LVS+F+ LL EENP+RE TIVRTA
Sbjct: 266  VLPLRDCFAQEINMLCHLDGLKTVKLSTDLCSSINGLVSSFVALLQEENPSRESTIVRTA 325

Query: 1067 EKLVPFQFNRINESLDS----GDRRRRRKVNLKSYESISSEFLCPICCSPVNNLDLEYLK 900
             KL PFQFNRI E +D       RRR+++ NLKS ES+SSE  CP+C SP++  ++    
Sbjct: 326  GKLTPFQFNRIPEIIDGNVPLATRRRQKRYNLKSNESVSSESFCPLCNSPLDKSEITDW- 384

Query: 899  SKLESCATSPTVFGDSCCPSCQFQILPKESSSMEHFYSLVPQQMITRVKDSVGGDRAWLR 720
            S ++S  +S  +F ++CC SC+FQILP +S +ME FY  +PQ ++ R K +  G+ + LR
Sbjct: 385  SNIDSHRSS-DIFYNTCCSSCRFQILPSDSMAMEQFYMDLPQSVVGRAKQANNGNLSLLR 443

Query: 719  EQIEDCLLSDGEDE 678
            EQI+D LLSDGEDE
Sbjct: 444  EQIQDYLLSDGEDE 457


>ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|222854006|gb|EEE91553.1|
            predicted protein [Populus trichocarpa]
          Length = 443

 Score =  460 bits (1184), Expect = e-127
 Identities = 246/402 (61%), Positives = 302/402 (75%), Gaps = 14/402 (3%)
 Frame = -2

Query: 1916 NTPRTCYKCKVAKFIE--STGG----LCGECFDRSLYGKFKQAVTTQNLISPTDNVLLAF 1755
            N+   C KCK    I   S  G     C +CF  ++YGKF+ AVT+Q +ISP+DNVL+AF
Sbjct: 34   NSNNLCLKCKTNSPITPGSLSGDEARFCADCFRSNIYGKFRLAVTSQAMISPSDNVLVAF 93

Query: 1754 SGGPSSRVALEFVHEMQNKAQKNRDASRDKSYPVFGVGVAFIDESAVTSLQLDK---AIQ 1584
            SGGPSSRVAL+FVH +Q++AQKN +AS+DKS PVFGVGVAFIDESAV  +  DK   AIQ
Sbjct: 94   SGGPSSRVALQFVHGLQDRAQKNFEASKDKSLPVFGVGVAFIDESAVYPVSSDKVDGAIQ 153

Query: 1583 DLRMIVSTLAPPRKELHIMPIQTILSHDD-DAVNRLKELLLTVTDVTGKEDLLLHLRMLC 1407
            D+R +VS LAPP K+LH++PI+ I + D  D  +RL +LL  V D TGKEDLLLHLRML 
Sbjct: 154  DMRSVVSNLAPPTKQLHVVPIENIYASDSSDGKDRLTKLLDAVNDATGKEDLLLHLRMLA 213

Query: 1406 LQKVALENNYNKLILGSCTSRIACHVLSATVKGQGYSLPADIQYADARWEIPVVLPLRDC 1227
            LQ VA +N YN+LILGSCTSRIACHVL+ATVKGQGYSL ADIQY DARWEIPVVLPLRDC
Sbjct: 214  LQMVASQNGYNRLILGSCTSRIACHVLAATVKGQGYSLSADIQYVDARWEIPVVLPLRDC 273

Query: 1226 VSQELNMLCRLNSLKTLDSFDSPRSGINELVSTFITLLTEENPARERTIVRTAEKLVPFQ 1047
             SQELN+LC+L+ LKTL  FDS  SGIN L+S+F+ +L EEN +RE TI+RTA KL PF+
Sbjct: 274  TSQELNLLCQLDGLKTLQPFDSTPSGINGLISSFVNVLQEENSSRECTILRTAGKLTPFE 333

Query: 1046 FNRINE----SLDSGDRRRRRKVNLKSYESISSEFLCPICCSPVNNLDLEYLKSKLESCA 879
            FNRI E    ++    RRR+++ NLK+ ESISSE  CPIC +P+ N +   L S L+SC 
Sbjct: 334  FNRIPETDNCNVPLAIRRRQKRYNLKTKESISSELFCPICNAPLKNFNSLNL-SSLKSCQ 392

Query: 878  TSPTVFGDSCCPSCQFQILPKESSSMEHFYSLVPQQMITRVK 753
            +S   F  +CC SCQFQI+PK+ SSM +F SL+PQQ++ R K
Sbjct: 393  SSR--FNAACCSSCQFQIIPKDPSSMGNFCSLLPQQVVDRAK 432


>ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabidopsis thaliana]
            gi|332278212|sp|O65628.3|CTU2_ARATH RecName:
            Full=Cytoplasmic tRNA 2-thiolation protein 2
            gi|115646788|gb|ABJ17118.1| At4g35910 [Arabidopsis
            thaliana] gi|332661188|gb|AEE86588.1| cytoplasmic tRNA
            2-thiolation protein 2 [Arabidopsis thaliana]
          Length = 458

 Score =  422 bits (1084), Expect = e-115
 Identities = 228/428 (53%), Positives = 302/428 (70%), Gaps = 14/428 (3%)
 Frame = -2

Query: 1916 NTPRTCYKCKV-AKFIESTGGL-----CGECFDRSLYGKFKQAVTTQNLISPTDNVLLAF 1755
            N    C KCK  A      GG      C +CF  +++GKF+ AVT+  +I+P+DNVL+AF
Sbjct: 33   NHESVCVKCKCNAPMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAF 92

Query: 1754 SGGPSSRVALEFVHEMQNKAQKNRDASRDKSYPVFGVGVAFIDESAV---TSLQLDKAIQ 1584
            SGG SSRV+L+FVHE+Q KA KN +ASRD+S PVFGVGVAF+DE+A     S ++  AI+
Sbjct: 93   SGGSSSRVSLQFVHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIE 152

Query: 1583 DLRMIVSTLAPPRKELHIMPIQTILSHDD-DAVNRLKELLLTVTDVTGKEDLLLHLRMLC 1407
             +R  VS L+PP K+LH++P+++I   D  DA +RL +LL +V D TGKEDLLLHL+ML 
Sbjct: 153  WVRYTVSCLSPPAKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLS 212

Query: 1406 LQKVALENNYNKLILGSCTSRIACHVLSATVKGQGYSLPADIQYADARWEIPVVLPLRDC 1227
            LQKVA EN YN+L+LGSCTSRIA HVL+ATVKG+GYSL ADIQ+ DARW++P+VLPLRDC
Sbjct: 213  LQKVAAENGYNRLVLGSCTSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDC 272

Query: 1226 VSQELNMLCRLNSLKTLDSFDSPRSGINELVSTFITLLTEENPARERTIVRTAEKLVPFQ 1047
            V  E+  LC L+ LKT++     + GIN+LVS+F+ LL EENP+RE TIVRTA KL PF 
Sbjct: 273  VRLEITRLCLLDGLKTVELACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFY 332

Query: 1046 FNRINESLDS----GDRRRRRKVNLKSYESISSEFLCPICCSPVNNLDLEYLKSKLESCA 879
            FN+I E+ DS      +RR ++ NLK   S+++E  CPIC  P+N  D   L +  E   
Sbjct: 333  FNKIPETDDSNVPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDT-FEEGQ 391

Query: 878  TSPTVFGDSCCPSCQFQILPKESSSMEHFYSLVPQQMITRVKDSVGGDRAWLREQIEDCL 699
             S  ++  +CC SC+FQILP++ SS+E F S +P  MI++VK      +A+LRE+I+DCL
Sbjct: 392  ESDVLYA-ACCSSCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCL 450

Query: 698  LSDGEDEV 675
            L D E+ V
Sbjct: 451  LLDDEEVV 458


>ref|XP_003553411.1| PREDICTED: LOW QUALITY PROTEIN: cytoplasmic tRNA 2-thiolation protein
            2-like, partial [Glycine max]
          Length = 389

 Score =  420 bits (1079), Expect = e-115
 Identities = 222/388 (57%), Positives = 286/388 (73%), Gaps = 7/388 (1%)
 Frame = -2

Query: 1862 GGLCGECFDRSLYGKFKQAVTTQNLISPTDNVLLAFSGGPSSRVALEFVHEMQNKAQKNR 1683
            G  C +CF  +L+GKF+ AVT+  +ISPTD VL+AFSGGPSSRVAL+FVH+MQ +AQ+N 
Sbjct: 7    GRFCADCFKTNLFGKFRSAVTSNAMISPTDKVLVAFSGGPSSRVALQFVHDMQERAQRNF 66

Query: 1682 DASRDKSYPVFGVGVAFIDESAVTSL---QLDKAIQDLRMIVSTLAPPRKELHIMPIQTI 1512
            DASRD+S PVFGVGV FI+ES V  +   ++++A+  +  +VS+LA PRKEL I+PI T 
Sbjct: 67   DASRDRSLPVFGVGVVFINESTVLPIPSNEMEEAVGVVSEVVSSLATPRKELRIVPIDTA 126

Query: 1511 LSHDDDAVNRLKELLLTVTDVTGKEDLLLHLRMLCLQKVALENNYNKLILGSCTSRIACH 1332
             S D D     KE +  V D TG+ED+LL LRML LQKVA E  YN++ILGSC SRIACH
Sbjct: 127  YSSDSDDG---KERIAKVXDPTGREDVLLCLRMLALQKVASEFGYNRIILGSCISRIACH 183

Query: 1331 VLSATVKGQGYSLPADIQYADARWEIPVVLPLRDCVSQELNMLCRLNSLKTLDSFDSPRS 1152
            V+SATVKGQGYSLPADIQY DARWE+PVVLPLRDC +QE+NMLCRL+ LKT+       S
Sbjct: 184  VISATVKGQGYSLPADIQYVDARWEVPVVLPLRDCFAQEINMLCRLDGLKTVKLSTDLCS 243

Query: 1151 GINELVSTFITLLTEENPARERTIVRTAEKLVPFQFNRINESLDSG----DRRRRRKVNL 984
             IN LVS+F+ LL EENP+RE +IVRTA KL PFQFNRI E +DS      RR +++ NL
Sbjct: 244  SINGLVSSFVALLQEENPSRESSIVRTAGKLTPFQFNRIPEIIDSNVPLTTRRCQKRYNL 303

Query: 983  KSYESISSEFLCPICCSPVNNLDLEYLKSKLESCATSPTVFGDSCCPSCQFQILPKESSS 804
            KS ES+SSE  CP+C SP++  ++    S ++S  +S  +F ++CC SC+FQILP +S++
Sbjct: 304  KSNESVSSESFCPLCNSPLDKSEIVDW-SNIDSHRSS-DIFYNTCCSSCRFQILPSDSTT 361

Query: 803  MEHFYSLVPQQMITRVKDSVGGDRAWLR 720
            ME FY   PQ +++R K +  G+ + LR
Sbjct: 362  MEQFYMDFPQSVVSRPKQANNGNLSLLR 389