BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00003884
         (2050 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003633097.1| PREDICTED: pentatricopeptide repeat-containi...   449   e-123
emb|CBI27939.3| unnamed protein product [Vitis vinifera]              449   e-123
ref|XP_002330519.1| predicted protein [Populus trichocarpa] gi|2...   441   e-121
ref|XP_004147002.1| PREDICTED: putative pentatricopeptide repeat...   413   e-112
ref|XP_004158941.1| PREDICTED: putative pentatricopeptide repeat...   405   e-110

>ref|XP_003633097.1| PREDICTED: pentatricopeptide repeat-containing protein At4g13650-like
            [Vitis vinifera]
          Length = 1005

 Score =  449 bits (1155), Expect = e-123
 Identities = 209/326 (64%), Positives = 256/326 (78%)
 Frame = -1

Query: 2047 KCGCIKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIG 1868
            KCGC++D E VF  LVSRD VSWN+ ICGY+Q+G G KAL AF+ MLDEG  PDE+TFIG
Sbjct: 680  KCGCVEDAEVVFDGLVSRDTVSWNTIICGYSQHGQGGKALKAFEAMLDEGTVPDEVTFIG 739

Query: 1867 VLSACSHVGLIKEGKYHFESMSKVYGITPTLKHYACMVDILGRAGRLDEVQNFIEKMPFV 1688
            VLSACSH+GLI+EGK HF S+SK+YGITPT++HYACMVDILGRAG+  EV++FIE+M   
Sbjct: 740  VLSACSHMGLIEEGKKHFNSLSKIYGITPTIEHYACMVDILGRAGKFHEVESFIEEMKLT 799

Query: 1687 PDSLIWQTVLGACVRHGNVEMAEKAAEELFILEPQMDSTYILLSNIYAAKRRWDDVSKVR 1508
             + LIW+TVLGAC  HGN+E  E+AA +LF LEP++DS YILLSN++AAK  WDDV+ VR
Sbjct: 800  SNVLIWETVLGACKMHGNIEFGERAAMKLFELEPEIDSNYILLSNMFAAKGMWDDVTNVR 859

Query: 1507 ERMSSQGVKKEPGCSWVEIDGQVHVFVSRDVSHPNVKKIYQKLDEMGQELMLAGYVPDTD 1328
              MS++GVKKEPGCSWVE++GQVHVF+S D SHP +++I+ KL ++ Q+LM  GY P+TD
Sbjct: 860  ALMSTRGVKKEPGCSWVEVNGQVHVFLSHDGSHPKIREIHLKLQDLHQKLMSVGYTPNTD 919

Query: 1327 SVLHNVSDAEKKESLLYHSERLALAFSLISTPPGKQIRIFKNLRICRDCHNALKLXXXXX 1148
             VLHNVSD EK+E L YHSERLALAF+L+ST   K IRIFKNLRIC DCH+ +K      
Sbjct: 920  HVLHNVSDREKQELLFYHSERLALAFALLSTSTRKTIRIFKNLRICGDCHDFMKSISEIT 979

Query: 1147 XXXXXXXXXXRFHHFQNGSCSCCDFW 1070
                       FHHF+NGSCSC +FW
Sbjct: 980  NQELVVRDINCFHHFKNGSCSCQNFW 1005



 Score = 79.7 bits (195), Expect = 3e-12
 Identities = 43/145 (29%), Positives = 75/145 (51%)
 Frame = -1

Query: 2035 IKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIGVLSA 1856
            ++D E +F+ L+ RD+ +W   + GYAQ G GEKA+  F  M  EG  P+E T    LS 
Sbjct: 583  LEDAETIFNRLIKRDLFAWTVIVAGYAQDGQGEKAVKCFIQMQREGVKPNEFTLASSLSG 642

Query: 1855 CSHVGLIKEGKYHFESMSKVYGITPTLKHYACMVDILGRAGRLDEVQNFIEKMPFVPDSL 1676
            CS +  +  G+    SM+   G +  +   + +VD+  + G +++ +   + +    D++
Sbjct: 643  CSRIATLDSGR-QLHSMAIKAGQSGDMFVASALVDMYAKCGCVEDAEVVFDGL-VSRDTV 700

Query: 1675 IWQTVLGACVRHGNVEMAEKAAEEL 1601
             W T++    +HG    A KA E +
Sbjct: 701  SWNTIICGYSQHGQGGKALKAFEAM 725



 Score = 62.8 bits (151), Expect = 3e-07
 Identities = 42/140 (30%), Positives = 63/140 (45%)
 Frame = -1

Query: 2047 KCGCIKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIG 1868
            KCG       VF  +  RD+VSW + I G+   GYG  A+N F  M  EG   +E T+  
Sbjct: 175  KCGSANYACKVFGEIPERDVVSWTALITGFVAEGYGSGAVNLFCEMRREGVEANEFTYAT 234

Query: 1867 VLSACSHVGLIKEGKYHFESMSKVYGITPTLKHYACMVDILGRAGRLDEVQNFIEKMPFV 1688
             L ACS    ++ GK       KV G    L   + +VD+  + G +   +     MP  
Sbjct: 235  ALKACSMCLDLEFGKQVHAEAIKV-GDFSDLFVGSALVDLYAKCGEMVLAERVFLCMP-K 292

Query: 1687 PDSLIWQTVLGACVRHGNVE 1628
             +++ W  +L    + G+ E
Sbjct: 293  QNAVSWNALLNGFAQMGDAE 312


>emb|CBI27939.3| unnamed protein product [Vitis vinifera]
          Length = 764

 Score =  449 bits (1155), Expect = e-123
 Identities = 209/326 (64%), Positives = 256/326 (78%)
 Frame = -1

Query: 2047 KCGCIKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIG 1868
            KCGC++D E VF  LVSRD VSWN+ ICGY+Q+G G KAL AF+ MLDEG  PDE+TFIG
Sbjct: 439  KCGCVEDAEVVFDGLVSRDTVSWNTIICGYSQHGQGGKALKAFEAMLDEGTVPDEVTFIG 498

Query: 1867 VLSACSHVGLIKEGKYHFESMSKVYGITPTLKHYACMVDILGRAGRLDEVQNFIEKMPFV 1688
            VLSACSH+GLI+EGK HF S+SK+YGITPT++HYACMVDILGRAG+  EV++FIE+M   
Sbjct: 499  VLSACSHMGLIEEGKKHFNSLSKIYGITPTIEHYACMVDILGRAGKFHEVESFIEEMKLT 558

Query: 1687 PDSLIWQTVLGACVRHGNVEMAEKAAEELFILEPQMDSTYILLSNIYAAKRRWDDVSKVR 1508
             + LIW+TVLGAC  HGN+E  E+AA +LF LEP++DS YILLSN++AAK  WDDV+ VR
Sbjct: 559  SNVLIWETVLGACKMHGNIEFGERAAMKLFELEPEIDSNYILLSNMFAAKGMWDDVTNVR 618

Query: 1507 ERMSSQGVKKEPGCSWVEIDGQVHVFVSRDVSHPNVKKIYQKLDEMGQELMLAGYVPDTD 1328
              MS++GVKKEPGCSWVE++GQVHVF+S D SHP +++I+ KL ++ Q+LM  GY P+TD
Sbjct: 619  ALMSTRGVKKEPGCSWVEVNGQVHVFLSHDGSHPKIREIHLKLQDLHQKLMSVGYTPNTD 678

Query: 1327 SVLHNVSDAEKKESLLYHSERLALAFSLISTPPGKQIRIFKNLRICRDCHNALKLXXXXX 1148
             VLHNVSD EK+E L YHSERLALAF+L+ST   K IRIFKNLRIC DCH+ +K      
Sbjct: 679  HVLHNVSDREKQELLFYHSERLALAFALLSTSTRKTIRIFKNLRICGDCHDFMKSISEIT 738

Query: 1147 XXXXXXXXXXRFHHFQNGSCSCCDFW 1070
                       FHHF+NGSCSC +FW
Sbjct: 739  NQELVVRDINCFHHFKNGSCSCQNFW 764



 Score = 79.7 bits (195), Expect = 3e-12
 Identities = 43/145 (29%), Positives = 75/145 (51%)
 Frame = -1

Query: 2035 IKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIGVLSA 1856
            ++D E +F+ L+ RD+ +W   + GYAQ G GEKA+  F  M  EG  P+E T    LS 
Sbjct: 342  LEDAETIFNRLIKRDLFAWTVIVAGYAQDGQGEKAVKCFIQMQREGVKPNEFTLASSLSG 401

Query: 1855 CSHVGLIKEGKYHFESMSKVYGITPTLKHYACMVDILGRAGRLDEVQNFIEKMPFVPDSL 1676
            CS +  +  G+    SM+   G +  +   + +VD+  + G +++ +   + +    D++
Sbjct: 402  CSRIATLDSGR-QLHSMAIKAGQSGDMFVASALVDMYAKCGCVEDAEVVFDGL-VSRDTV 459

Query: 1675 IWQTVLGACVRHGNVEMAEKAAEEL 1601
             W T++    +HG    A KA E +
Sbjct: 460  SWNTIICGYSQHGQGGKALKAFEAM 484



 Score = 59.7 bits (143), Expect = 3e-06
 Identities = 40/144 (27%), Positives = 62/144 (43%)
 Frame = -1

Query: 2047 KCGCIKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIG 1868
            KCG       VF  +  RD+VSW + I G+   GYG   L  F  ML EG  P+  TFI 
Sbjct: 238  KCGSANYACKVFGEIPERDVVSWTALITGFVAEGYG-SGLRIFNQMLAEGFNPNMYTFIS 296

Query: 1867 VLSACSHVGLIKEGKYHFESMSKVYGITPTLKHYACMVDILGRAGRLDEVQNFIEKMPFV 1688
            +L +CS +  +  GK     + K   +         +VD+  +   L++ +    ++   
Sbjct: 297  ILRSCSSLSDVDLGKQVHAQIVK-NSLDGNDFVGTALVDMYAKNRFLEDAETIFNRL-IK 354

Query: 1687 PDSLIWQTVLGACVRHGNVEMAEK 1616
             D   W  ++    + G  E A K
Sbjct: 355  RDLFAWTVIVAGYAQDGQGEKAVK 378


>ref|XP_002330519.1| predicted protein [Populus trichocarpa] gi|222872453|gb|EEF09584.1|
            predicted protein [Populus trichocarpa]
          Length = 726

 Score =  441 bits (1135), Expect = e-121
 Identities = 208/327 (63%), Positives = 257/327 (78%)
 Frame = -1

Query: 2050 GKCGCIKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFI 1871
            GKCGC++  EA+F  L+SRDIVSWN+ I GY+Q+G GEKAL AF+ ML EG  PDE TFI
Sbjct: 400  GKCGCMEHAEAIFKGLISRDIVSWNTIISGYSQHGQGEKALEAFRMMLSEGIMPDEATFI 459

Query: 1870 GVLSACSHVGLIKEGKYHFESMSKVYGITPTLKHYACMVDILGRAGRLDEVQNFIEKMPF 1691
            GVLSACS +GL++EGK  F+SMSK+YGI P+++HYACMVDILGRAG+ +EV+ FIE+M  
Sbjct: 460  GVLSACSFMGLVEEGKKRFDSMSKIYGINPSIEHYACMVDILGRAGKFNEVKIFIEEMNL 519

Query: 1690 VPDSLIWQTVLGACVRHGNVEMAEKAAEELFILEPQMDSTYILLSNIYAAKRRWDDVSKV 1511
             P SLIW+TVLGAC  HGNV+  EKAA++LF +EP MDS+YILLSNI+A+K RWDDV  +
Sbjct: 520  TPYSLIWETVLGACKLHGNVDFGEKAAKKLFEMEPMMDSSYILLSNIFASKGRWDDVRNI 579

Query: 1510 RERMSSQGVKKEPGCSWVEIDGQVHVFVSRDVSHPNVKKIYQKLDEMGQELMLAGYVPDT 1331
            R  M+S+G+KKEPGCSWVE+DGQVHVF+S+D SHP +++IY KLD++GQ LM  GYVP T
Sbjct: 580  RALMTSRGIKKEPGCSWVEVDGQVHVFLSQDGSHPKIREIYAKLDKLGQSLMSIGYVPKT 639

Query: 1330 DSVLHNVSDAEKKESLLYHSERLALAFSLISTPPGKQIRIFKNLRICRDCHNALKLXXXX 1151
            + VLHNVS+ EK E L YHSERLAL+F+L+ST   K IRIFKNLRIC DCH+ +KL    
Sbjct: 640  EVVLHNVSNKEKMEHLYYHSERLALSFALLSTNAVKPIRIFKNLRICEDCHDFMKLISDI 699

Query: 1150 XXXXXXXXXXXRFHHFQNGSCSCCDFW 1070
                       RFHHF+ G+CSC D W
Sbjct: 700  TNQEIVVRDIRRFHHFKRGTCSCQDRW 726



 Score = 84.3 bits (207), Expect = 1e-13
 Identities = 54/194 (27%), Positives = 94/194 (48%), Gaps = 15/194 (7%)
 Frame = -1

Query: 2047 KCGCIKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIG 1868
            K  C++D    F  LV+RDI SW   I GYAQ    EKA+  F+ M  EG  P+E T   
Sbjct: 300  KARCLEDAGVAFDRLVNRDIFSWTVIISGYAQTDQAEKAVKYFRQMQREGIKPNEYTLAS 359

Query: 1867 VLSACSHVGLIKEGKYHFESMSKVYGITPTLKHY------ACMVDILGRAGRLDEVQNFI 1706
             LS CSH+  ++ G+       +++ +     H+      + +VD+ G+ G ++  +   
Sbjct: 360  CLSGCSHMATLENGR-------QLHAVAVKAGHFGDIFVGSALVDLYGKCGCMEHAEAIF 412

Query: 1705 EKMPFVPDSLIWQTVLGACVRHGNVEMAEKAAEELFILEPQM--DSTYI-------LLSN 1553
            + +    D + W T++    +HG  E A +A   + + E  M  ++T+I        +  
Sbjct: 413  KGL-ISRDIVSWNTIISGYSQHGQGEKALEAF-RMMLSEGIMPDEATFIGVLSACSFMGL 470

Query: 1552 IYAAKRRWDDVSKV 1511
            +   K+R+D +SK+
Sbjct: 471  VEEGKKRFDSMSKI 484


>ref|XP_004147002.1| PREDICTED: putative pentatricopeptide repeat-containing protein
            At5g09950-like [Cucumis sativus]
          Length = 989

 Score =  413 bits (1061), Expect = e-112
 Identities = 193/326 (59%), Positives = 247/326 (75%)
 Frame = -1

Query: 2047 KCGCIKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIG 1868
            KCGC+++ EA+F  L+ RD ++WN+ ICGYAQ G G KAL AF+ MLDEG +PD +TF G
Sbjct: 664  KCGCMEEAEALFEALIRRDTIAWNTIICGYAQNGQGNKALTAFRMMLDEGISPDGVTFTG 723

Query: 1867 VLSACSHVGLIKEGKYHFESMSKVYGITPTLKHYACMVDILGRAGRLDEVQNFIEKMPFV 1688
            +LSACSH GL++EGK HF SM + +GI+PT+ H ACMVDILGR G+ DE+++FI+KM   
Sbjct: 724  ILSACSHQGLVEEGKEHFNSMYRDFGISPTVDHCACMVDILGRVGKFDELEDFIQKMQLS 783

Query: 1687 PDSLIWQTVLGACVRHGNVEMAEKAAEELFILEPQMDSTYILLSNIYAAKRRWDDVSKVR 1508
             ++LIW+TVLGA   H N+ + EKAA +LF L+P+ +S+YILLSNI+A + RWDDV +VR
Sbjct: 784  QNALIWETVLGASKMHNNLVLGEKAANKLFELQPEEESSYILLSNIFATEGRWDDVKRVR 843

Query: 1507 ERMSSQGVKKEPGCSWVEIDGQVHVFVSRDVSHPNVKKIYQKLDEMGQELMLAGYVPDTD 1328
              MSS+GVKKEPGCSWVE +GQVH FVS D SHP +++I+ KLDE+ +EL    YVP T+
Sbjct: 844  SLMSSKGVKKEPGCSWVEANGQVHTFVSHDYSHPQIQEIHLKLDELDRELASIQYVPKTE 903

Query: 1327 SVLHNVSDAEKKESLLYHSERLALAFSLISTPPGKQIRIFKNLRICRDCHNALKLXXXXX 1148
             VLHNV + EKKE+L +HSERLAL F+LIST   K+IRIFKNLRICRDCH+ +K      
Sbjct: 904  YVLHNVGETEKKENLRFHSERLALGFALISTSSEKKIRIFKNLRICRDCHDVMKHISSIT 963

Query: 1147 XXXXXXXXXXRFHHFQNGSCSCCDFW 1070
                      RFHHF+NG+CSC DFW
Sbjct: 964  NQEIVVRDVRRFHHFKNGACSCNDFW 989



 Score = 79.0 bits (193), Expect = 5e-12
 Identities = 44/137 (32%), Positives = 69/137 (50%)
 Frame = -1

Query: 2047 KCGCIKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIG 1868
            KC  ++D +  F+ L  RD+ +W   I  YAQ   GEKALN F+ M  EG  P+E T  G
Sbjct: 563  KCMYLEDADVAFNRLSVRDLFTWTVIITNYAQTNQGEKALNYFRQMQQEGVKPNEFTLAG 622

Query: 1867 VLSACSHVGLIKEGKYHFESMSKVYGITPTLKHYACMVDILGRAGRLDEVQNFIEKMPFV 1688
             LS CS +  + EG     SM    G    +   + +VD+  + G ++E +   E +   
Sbjct: 623  CLSGCSSLASL-EGGQQLHSMVFKSGHVSDMFVGSALVDMYAKCGCMEEAEALFEAL-IR 680

Query: 1687 PDSLIWQTVLGACVRHG 1637
             D++ W T++    ++G
Sbjct: 681  RDTIAWNTIICGYAQNG 697



 Score = 60.1 bits (144), Expect = 2e-06
 Identities = 25/66 (37%), Positives = 41/66 (62%)
 Frame = -1

Query: 2047 KCGCIKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIG 1868
            K GC+ D   ++ ++V RD++SWN+++ G    G  ++ L  F  ML+EG  P+  TFI 
Sbjct: 462  KNGCVHDGTKLYESMVDRDLISWNAYLSGLHDCGMYDRPLTIFYHMLEEGFIPNMYTFIS 521

Query: 1867 VLSACS 1850
            +L +CS
Sbjct: 522  ILGSCS 527


>ref|XP_004158941.1| PREDICTED: putative pentatricopeptide repeat-containing protein
            At5g09950-like [Cucumis sativus]
          Length = 1004

 Score =  405 bits (1041), Expect = e-110
 Identities = 190/322 (59%), Positives = 244/322 (75%)
 Frame = -1

Query: 2047 KCGCIKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIG 1868
            KCGC+++ EA+F  L+ RD ++WN+ ICGYAQ G G KAL AF+ MLDEG +PD +TF G
Sbjct: 664  KCGCMEEAEALFEALIRRDTIAWNTIICGYAQNGQGNKALTAFRMMLDEGISPDGVTFTG 723

Query: 1867 VLSACSHVGLIKEGKYHFESMSKVYGITPTLKHYACMVDILGRAGRLDEVQNFIEKMPFV 1688
            +LSACSH GL++EGK HF SM + +GI+PT+ H ACMVDILGR G+ DE+++FI+KM   
Sbjct: 724  ILSACSHQGLVEEGKEHFNSMYRDFGISPTVDHCACMVDILGRVGKFDELEDFIQKMQLS 783

Query: 1687 PDSLIWQTVLGACVRHGNVEMAEKAAEELFILEPQMDSTYILLSNIYAAKRRWDDVSKVR 1508
             ++LIW+TVLGA   H N+ + EKAA +LF L+P+ +S+YILLSNI+A + RWDDV +VR
Sbjct: 784  QNALIWETVLGASKMHNNLVLGEKAANKLFELQPEEESSYILLSNIFATEGRWDDVKRVR 843

Query: 1507 ERMSSQGVKKEPGCSWVEIDGQVHVFVSRDVSHPNVKKIYQKLDEMGQELMLAGYVPDTD 1328
              MSS+GVKKEPGCSWVE +GQVH FVS D SHP +++I+ KLDE+ +EL    YVP T+
Sbjct: 844  SLMSSKGVKKEPGCSWVEANGQVHTFVSHDYSHPQIQEIHLKLDELDRELASIQYVPKTE 903

Query: 1327 SVLHNVSDAEKKESLLYHSERLALAFSLISTPPGKQIRIFKNLRICRDCHNALKLXXXXX 1148
             VLHNV + EKKE+L +HSERLAL F+LIST   K+IRIFKNLRICRDCH+ +K      
Sbjct: 904  YVLHNVGETEKKENLRFHSERLALGFALISTSSEKKIRIFKNLRICRDCHDVMKHISSIT 963

Query: 1147 XXXXXXXXXXRFHHFQNGSCSC 1082
                      RFHHF+NG+CSC
Sbjct: 964  NQEIVVRDVRRFHHFKNGACSC 985



 Score = 79.0 bits (193), Expect = 5e-12
 Identities = 44/137 (32%), Positives = 69/137 (50%)
 Frame = -1

Query: 2047 KCGCIKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIG 1868
            KC  ++D +  F+ L  RD+ +W   I  YAQ   GEKALN F+ M  EG  P+E T  G
Sbjct: 563  KCMYLEDADVAFNRLSVRDLFTWTVIITNYAQTNQGEKALNYFRQMQQEGVKPNEFTLAG 622

Query: 1867 VLSACSHVGLIKEGKYHFESMSKVYGITPTLKHYACMVDILGRAGRLDEVQNFIEKMPFV 1688
             LS CS +  + EG     SM    G    +   + +VD+  + G ++E +   E +   
Sbjct: 623  CLSGCSSLASL-EGGQQLHSMVFKSGHVSDMFVGSALVDMYAKCGCMEEAEALFEAL-IR 680

Query: 1687 PDSLIWQTVLGACVRHG 1637
             D++ W T++    ++G
Sbjct: 681  RDTIAWNTIICGYAQNG 697



 Score = 60.1 bits (144), Expect = 2e-06
 Identities = 25/66 (37%), Positives = 41/66 (62%)
 Frame = -1

Query: 2047 KCGCIKDVEAVFSNLVSRDIVSWNSFICGYAQYGYGEKALNAFKGMLDEGATPDEITFIG 1868
            K GC+ D   ++ ++V RD++SWN+++ G    G  ++ L  F  ML+EG  P+  TFI 
Sbjct: 462  KNGCVHDGTKLYESMVDRDLISWNAYLSGLHDCGMYDRPLTIFYHMLEEGFIPNMYTFIS 521

Query: 1867 VLSACS 1850
            +L +CS
Sbjct: 522  ILGSCS 527