BLASTX nr result
ID: Coptis21_contig00003173
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00003173
(1222 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta] 435 e-120
ref|XP_002327096.1| cytochrome P450 [Populus trichocarpa] gi|222... 426 e-117
ref|XP_002282051.2| PREDICTED: cytochrome P450 82A3-like [Vitis ... 418 e-114
ref|XP_002268915.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif... 416 e-114
ref|XP_003634448.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P... 415 e-113
>dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 511
Score = 435 bits (1118), Expect(2) = e-120
Identities = 211/331 (63%), Positives = 250/331 (75%)
Frame = +1
Query: 1 VLVEMKQWFGDLTLNVVVRMVAGKRYMGGSIKSDDVEGRRFQKAIKDLFELFGLFVLSDA 180
VLV+MKQWFGDLTLNV++RM+AGKRY G D+ E R QK ++D F L GLF++ DA
Sbjct: 168 VLVDMKQWFGDLTLNVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDA 227
Query: 181 LPYLGWLDLHGHKKAMKQTAKELDSIMQRWLEEHRRNLLDDKTKEEKDFMYVLLSIIDDK 360
LPYL W DL G+KK MK TAKELDS+ QRWLEEH R + E+DFM VL+SI++D
Sbjct: 228 LPYLSWFDLQGYKKEMKNTAKELDSVFQRWLEEHNRMRQTGELNREQDFMDVLMSILEDT 287
Query: 361 KLFGYDADIANKAICLNMIIAGTDTQMITLTWVLSLLLNNRHILKKAQEEIDTSVGKDRQ 540
++ YD D K+ CL+++ G DT M+TLTW+LSLLLNN+H LKKAQ+E+D+ VGKDRQ
Sbjct: 288 RISEYDNDTIIKSTCLSIVTGGGDTTMVTLTWILSLLLNNKHALKKAQDELDSHVGKDRQ 347
Query: 541 VDESDIVKLVYLQAIVKEALRLYPPAPLSAQHEAMENCTVAGYYVPAGTRLITNIRKIQR 720
V+ESDI LVYLQAI KEALRLYP PLS A +CTVAGY+VPAGTRLI N KIQR
Sbjct: 348 VEESDIKNLVYLQAITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQR 407
Query: 721 DPRVWSNPGEFHPERFLTNQANVDFRGQYFEFIPFGSGRRICPGISFALQVVHLTLARFL 900
DP VWS P EF PERFLT+ N+D +G ++E IPFG+GRR CPG+SF LQVV L LARFL
Sbjct: 408 DPLVWSEPSEFRPERFLTSHVNMDVKGLHYELIPFGAGRRSCPGMSFTLQVVPLVLARFL 467
Query: 901 QSFDFKTPMDASVDMTEAPGLTNDKATPLQV 993
FD KT MD VDMTE GLTN KATPL+V
Sbjct: 468 HEFDSKTEMDMPVDMTETAGLTNAKATPLEV 498
Score = 23.9 bits (50), Expect(2) = e-120
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +2
Query: 1040 LITPRLHPNLY 1072
+ITPRLHP +Y
Sbjct: 499 VITPRLHPEIY 509
>ref|XP_002327096.1| cytochrome P450 [Populus trichocarpa] gi|222835411|gb|EEE73846.1|
cytochrome P450 [Populus trichocarpa]
Length = 392
Score = 426 bits (1095), Expect = e-117
Identities = 212/335 (63%), Positives = 260/335 (77%), Gaps = 4/335 (1%)
Frame = +1
Query: 1 VLVEMKQWFGDLTLNVVVRMVAGKRYMGGSIKSDDVEGRRFQKAIKDLFELFGLFVLSDA 180
V+VE+KQW DLTLNVVVRMVAGKRY G + SDD E RR QKAI F L G+FV+SDA
Sbjct: 45 VVVELKQWLEDLTLNVVVRMVAGKRYFGSAAASDDGEARRCQKAINQFFRLIGIFVVSDA 104
Query: 181 LPYLGWLDLHGHKKAMKQTAKELDSIMQRWLEEHRRNLLDDKTKEE--KDFMYVLLSIID 354
LP+LGWLDL GH++AMK TAKELD+I++ WL+EHR+ + K+E +DF+ V+LS+ +
Sbjct: 105 LPFLGWLDLQGHERAMKNTAKELDAILEGWLDEHRQRRVSAGIKDEGEQDFIDVMLSLKE 164
Query: 355 DKKL--FGYDADIANKAICLNMIIAGTDTQMITLTWVLSLLLNNRHILKKAQEEIDTSVG 528
+ +L F YDA+ + K+ CL +I+ G+DT TLTW +SLLLNNRH+LKKAQEE+D VG
Sbjct: 165 EGQLSNFQYDANTSIKSTCLALILGGSDTTAGTLTWAISLLLNNRHMLKKAQEELDLHVG 224
Query: 529 KDRQVDESDIVKLVYLQAIVKEALRLYPPAPLSAQHEAMENCTVAGYYVPAGTRLITNIR 708
K+RQV++SD+ LVYLQ I+KE LRLYP PL EAME+C VAGY+VPAGTRLI N+
Sbjct: 225 KERQVEDSDVKNLVYLQTIIKETLRLYPAGPLLGPREAMEDCKVAGYHVPAGTRLIVNVW 284
Query: 709 KIQRDPRVWSNPGEFHPERFLTNQANVDFRGQYFEFIPFGSGRRICPGISFALQVVHLTL 888
KIQRDPRVW+ F PERFLT+ +VD RGQ FE IPFGSGRR CPG+SFALQV+HLTL
Sbjct: 285 KIQRDPRVWTKTSAFLPERFLTSHGDVDVRGQQFELIPFGSGRRSCPGVSFALQVLHLTL 344
Query: 889 ARFLQSFDFKTPMDASVDMTEAPGLTNDKATPLQV 993
AR L SF+ TPMD VD+TE+ GLT KATPL+V
Sbjct: 345 ARLLHSFELATPMDQPVDLTESSGLTIPKATPLEV 379
>ref|XP_002282051.2| PREDICTED: cytochrome P450 82A3-like [Vitis vinifera]
Length = 525
Score = 418 bits (1074), Expect = e-114
Identities = 201/331 (60%), Positives = 253/331 (76%)
Frame = +1
Query: 1 VLVEMKQWFGDLTLNVVVRMVAGKRYMGGSIKSDDVEGRRFQKAIKDLFELFGLFVLSDA 180
V VEMKQWFGDLTLNV++RMVAGKRY S S++ + +R ++ ++ L GLF ++DA
Sbjct: 186 VSVEMKQWFGDLTLNVILRMVAGKRYFSASDASENKQAQRCRRVFREFCHLSGLFAVADA 245
Query: 181 LPYLGWLDLHGHKKAMKQTAKELDSIMQRWLEEHRRNLLDDKTKEEKDFMYVLLSIIDDK 360
+P+LGWLDL H+K +K+TAKE+DSI Q WLEEHRR + +DFM V+LS++D K
Sbjct: 246 IPFLGWLDLGRHEKTLKKTAKEMDSIAQEWLEEHRRRKDSGEVNSTQDFMDVMLSVLDSK 305
Query: 361 KLFGYDADIANKAICLNMIIAGTDTQMITLTWVLSLLLNNRHILKKAQEEIDTSVGKDRQ 540
L YDAD NKA CL +I+ G+DT ++TLTW LSLLLNNR LKKAQEE+D VGK+R
Sbjct: 306 NLGDYDADTVNKATCLALIVGGSDTTVVTLTWALSLLLNNRDTLKKAQEELDIQVGKERL 365
Query: 541 VDESDIVKLVYLQAIVKEALRLYPPAPLSAQHEAMENCTVAGYYVPAGTRLITNIRKIQR 720
V+E DI KLVYLQAIVKE LRLYPPA L + E+CT+ GY+V GTRLI N+ KIQ+
Sbjct: 366 VNEQDISKLVYLQAIVKETLRLYPPAALGGPRQFTEDCTLGGYHVSKGTRLILNLSKIQK 425
Query: 721 DPRVWSNPGEFHPERFLTNQANVDFRGQYFEFIPFGSGRRICPGISFALQVVHLTLARFL 900
DPR+W +P EF PERFLT ++D R ++FEFIPFG+GRR CPGI+FALQ++HLTLA FL
Sbjct: 426 DPRIWMSPTEFQPERFLTTHKDLDPREKHFEFIPFGAGRRACPGIAFALQMLHLTLANFL 485
Query: 901 QSFDFKTPMDASVDMTEAPGLTNDKATPLQV 993
Q+FDF TP +A VDM E+ GLTN K+TPL+V
Sbjct: 486 QAFDFSTPSNAQVDMCESLGLTNMKSTPLEV 516
>ref|XP_002268915.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
gi|147794787|emb|CAN66846.1| hypothetical protein
VITISV_002367 [Vitis vinifera]
Length = 528
Score = 416 bits (1069), Expect = e-114
Identities = 202/333 (60%), Positives = 253/333 (75%), Gaps = 2/333 (0%)
Frame = +1
Query: 1 VLVEMKQWFGDLTLNVVVRMVAGKRYMGGSIKSDDVEGRRFQKAIKDLFELFGLFVLSDA 180
V V+MK+WFGDLTLN+ VR+V GKRY G + + EGR QK I+D LFG+FVLSDA
Sbjct: 183 VFVDMKRWFGDLTLNLAVRLVGGKRYFGAGADTKEGEGRTCQKVIRDFAHLFGVFVLSDA 242
Query: 181 LPYLGWLDLHGHKKAMKQTAKELDSIMQRWLEEHR-RNLLDDKTKEEKDFMYVLLSIIDD 357
+P+L WLDL G+KKAMK+TAKELDS+ WL+EH+ + LL + K+++DFM V+L++++D
Sbjct: 243 IPFLSWLDLKGYKKAMKRTAKELDSLFGGWLQEHKEKRLLGGEGKDDQDFMDVMLTVLED 302
Query: 358 KKLFGYDADIANKAICLNMIIAGTDTQMITLTWVLSLLLNNRHILKKAQEEIDTSVGKDR 537
G+DAD NKA CLN+I+AG+DT +TLTW LSLLLN+ H+LKKAQ E+D VGKDR
Sbjct: 303 VNFSGFDADTVNKATCLNLILAGSDTTKVTLTWALSLLLNHPHVLKKAQAELDIQVGKDR 362
Query: 538 QVDESDIVKLVYLQAIVKEALRLYPPAPLSAQHEAMENCTV-AGYYVPAGTRLITNIRKI 714
QVDESD+ LVYLQAI+KE LRLYP +P+ H AME+CT+ AGY + AGT+++ N KI
Sbjct: 363 QVDESDVKNLVYLQAIIKETLRLYPASPIITLHAAMEDCTLAAGYNISAGTQIMVNAWKI 422
Query: 715 QRDPRVWSNPGEFHPERFLTNQANVDFRGQYFEFIPFGSGRRICPGISFALQVVHLTLAR 894
RD RVW NP EF PERF+T+ + D RGQ+FE IPFGSGRR CPGIS ALQVVH LA
Sbjct: 423 HRDERVWCNPKEFQPERFMTSHKDTDVRGQHFELIPFGSGRRSCPGISLALQVVHFALAS 482
Query: 895 FLQSFDFKTPMDASVDMTEAPGLTNDKATPLQV 993
L S++ P D VDMTE+ GLTN KATPL+V
Sbjct: 483 LLHSYEVTKPSDGDVDMTESLGLTNLKATPLEV 515
>ref|XP_003634448.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 82A3-like [Vitis
vinifera]
Length = 555
Score = 415 bits (1066), Expect = e-113
Identities = 200/331 (60%), Positives = 253/331 (76%)
Frame = +1
Query: 1 VLVEMKQWFGDLTLNVVVRMVAGKRYMGGSIKSDDVEGRRFQKAIKDLFELFGLFVLSDA 180
V VEMKQWFGDLTLNV++RMVAGKRY S S++ + +R ++ ++ F L GLF ++DA
Sbjct: 216 VSVEMKQWFGDLTLNVILRMVAGKRYFSASDASENKQAQRCRRVFREFFHLXGLFAVADA 275
Query: 181 LPYLGWLDLHGHKKAMKQTAKELDSIMQRWLEEHRRNLLDDKTKEEKDFMYVLLSIIDDK 360
+P+LGWLDL H+K +K+TAKE+DSI Q WLEEHRR + +DFM V+LS++D K
Sbjct: 276 IPFLGWLDLGRHEKTLKKTAKEMDSIAQEWLEEHRRRKDSGEVNSTQDFMDVMLSVLDGK 335
Query: 361 KLFGYDADIANKAICLNMIIAGTDTQMITLTWVLSLLLNNRHILKKAQEEIDTSVGKDRQ 540
L YDAD NKA CL +I+ G+DT ++TLTW LSLLLNNR LKKAQEE+D VGK+R
Sbjct: 336 ILGDYDADTVNKATCLALIVGGSDTTVVTLTWALSLLLNNRDTLKKAQEELDIQVGKERL 395
Query: 541 VDESDIVKLVYLQAIVKEALRLYPPAPLSAQHEAMENCTVAGYYVPAGTRLITNIRKIQR 720
V+E DI KLVYLQAIVKE LRLYPPA L + E+CT+ GY+V GT LI N+ KIQ+
Sbjct: 396 VNEQDISKLVYLQAIVKETLRLYPPAALGGPRQFTEDCTLGGYHVSKGTXLILNLSKIQK 455
Query: 721 DPRVWSNPGEFHPERFLTNQANVDFRGQYFEFIPFGSGRRICPGISFALQVVHLTLARFL 900
DPR+W + EF PERFLT ++D +G++FEFIPFG+GRR CPGI+FALQ++HLTLA FL
Sbjct: 456 DPRIWMSLTEFQPERFLTTHKDLDPQGKHFEFIPFGAGRRACPGIAFALQMLHLTLANFL 515
Query: 901 QSFDFKTPMDASVDMTEAPGLTNDKATPLQV 993
Q+FDF TP +A VDM E+ GLTN K+TPL+V
Sbjct: 516 QAFDFSTPSNARVDMCESLGLTNMKSTPLEV 546