BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00002907
         (1543 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc...   699   0.0  
gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   691   0.0  
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra...   613   e-173
dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransfe...   494   e-137
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   488   e-135

>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
            4'-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:3'-hydroxy-N-
            methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
            gi|9711916|dbj|BAB08005.1|
            S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
            4'-O-methyltransferase [Coptis japonica]
          Length = 350

 Score =  699 bits (1805), Expect = 0.0
 Identities = 350/350 (100%), Positives = 350/350 (100%)
 Frame = -3

Query: 1334 MAFHGKDDVLDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLP 1155
            MAFHGKDDVLDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLP
Sbjct: 1    MAFHGKDDVLDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLP 60

Query: 1154 VSDVNCDNLYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQ 975
            VSDVNCDNLYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQ
Sbjct: 61   VSDVNCDNLYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQ 120

Query: 974  KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 795
            KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS
Sbjct: 121  KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 180

Query: 794  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDM 615
            GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDM
Sbjct: 181  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDM 240

Query: 614  FKSVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 435
            FKSVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST
Sbjct: 241  FKSVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 300

Query: 434  RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 285
            RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP
Sbjct: 301  RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350


>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase [Coptis chinensis]
          Length = 350

 Score =  691 bits (1783), Expect = 0.0
 Identities = 344/350 (98%), Positives = 348/350 (99%)
 Frame = -3

Query: 1334 MAFHGKDDVLDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLP 1155
            MAFHGKDD LDIKAQAHVWKIIYGFADSLVLRCAVELGIVDI+DNNNQPMALADLASKLP
Sbjct: 1    MAFHGKDDALDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIVDNNNQPMALADLASKLP 60

Query: 1154 VSDVNCDNLYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQ 975
            VS+VNCDNLYRILRYLVKMEILRVEKSDDG+KKYALEPIATLLSRNAKRSMVPMILGMTQ
Sbjct: 61   VSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQ 120

Query: 974  KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 795
            KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS
Sbjct: 121  KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 180

Query: 794  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDM 615
            GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYD PNIERIGGDM
Sbjct: 181  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM 240

Query: 614  FKSVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 435
            FKSVPSAQAI+LKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST
Sbjct: 241  FKSVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 300

Query: 434  RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 285
            RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP
Sbjct: 301  RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350


>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase [Thalictrum
            flavum subsp. glaucum]
          Length = 348

 Score =  613 bits (1581), Expect = e-173
 Identities = 309/350 (88%), Positives = 324/350 (92%), Gaps = 1/350 (0%)
 Frame = -3

Query: 1331 AFHGKDDVLDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPV 1152
            AF GKD  +DIKAQAHVWKIIYGFADSLVLRCAVELGI DIIDNNNQP+ LADLASKLP+
Sbjct: 3    AFQGKDASVDIKAQAHVWKIIYGFADSLVLRCAVELGIFDIIDNNNQPIPLADLASKLPI 62

Query: 1151 SDVNCDNLYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQK 972
            S+VN DNL+RILRYLVKME+L     D    KYALEPIA L+ RN KRSMVPM+LGMTQK
Sbjct: 63   SNVNLDNLFRILRYLVKMELLSYAADD----KYALEPIAKLVLRNEKRSMVPMVLGMTQK 118

Query: 971  DFMTPWHSMKDGLSDNG-TAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 795
            DFMTPWHSMKDGL+DNG TAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS
Sbjct: 119  DFMTPWHSMKDGLTDNGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 178

Query: 794  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDM 615
            GS+DMFQ IDSLVDVGGGNGTT+KAIS AFPHIKCTLFDLPHVIANSYD PNIERIGGDM
Sbjct: 179  GSKDMFQSIDSLVDVGGGNGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM 238

Query: 614  FKSVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 435
            FK +PSAQAI+LKLILHDWNDEDSIKILKQCR AVPKDGGKVIIVDVALDE+SDHELSST
Sbjct: 239  FKGMPSAQAILLKLILHDWNDEDSIKILKQCRKAVPKDGGKVIIVDVALDEDSDHELSST 298

Query: 434  RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 285
            RLILDIDMLVNTGGKERTKE WEK+VK AGFSGCKIRHIAAIQSVIEVFP
Sbjct: 299  RLILDIDMLVNTGGKERTKEDWEKLVKCAGFSGCKIRHIAAIQSVIEVFP 348


>dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase [Eschscholzia
            californica]
          Length = 353

 Score =  494 bits (1272), Expect = e-137
 Identities = 235/348 (67%), Positives = 297/348 (85%), Gaps = 4/348 (1%)
 Frame = -3

Query: 1316 DDVLDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNC 1137
            ++ +DIKAQAH+W IIYGFADSLVLR AVELGI DII NNN  + +++LASKLP+S+VN 
Sbjct: 6    NEEVDIKAQAHLWNIIYGFADSLVLRSAVELGIADIIKNNNGSITVSELASKLPISNVNS 65

Query: 1136 DNLYRILRYLVKMEILRVEKSD----DGQKKYALEPIATLLSRNAKRSMVPMILGMTQKD 969
            DNLYR+LRYLV M IL+  KS     + +K Y+LEP+ +LL ++A+R+MVP++LGMTQ+D
Sbjct: 66   DNLYRVLRYLVHMGILKETKSTINGGEIKKLYSLEPVGSLLVKDAERNMVPIVLGMTQQD 125

Query: 968  FMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGS 789
            FM PWH +K+GL +  TAFEK MGMT+WEYLEGHP+Q  LFN GM GETRLLT +LI   
Sbjct: 126  FMIPWHYIKEGLGEGSTAFEKGMGMTLWEYLEGHPEQGHLFNVGMEGETRLLTKTLIESC 185

Query: 788  RDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFK 609
            +D F+G+ SLVDVGGGNGTT+KAIS+AFPHIKC+L+DLPHV+A+S+DLPNIE+I GD+FK
Sbjct: 186  KDTFEGLSSLVDVGGGNGTTIKAISEAFPHIKCSLYDLPHVVADSHDLPNIEKIPGDIFK 245

Query: 608  SVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRL 429
             +P+AQAI+LKLILHDW+DEDS+KILK+CR AVP+D G+VIIVDVAL+EES+H L+ TRL
Sbjct: 246  FIPNAQAILLKLILHDWSDEDSVKILKKCREAVPQDTGRVIIVDVALEEESEHPLTKTRL 305

Query: 428  ILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 285
            +LD+DMLVNTGG+ER+++ W K++K AGF   KIRHIAA+QSVIE FP
Sbjct: 306  VLDVDMLVNTGGRERSEDDWAKLLKLAGFRTHKIRHIAAVQSVIEAFP 353


>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase 2 [Papaver somniferum]
          Length = 357

 Score =  488 bits (1255), Expect = e-135
 Identities = 239/343 (69%), Positives = 288/343 (83%), Gaps = 2/343 (0%)
 Frame = -3

Query: 1307 LDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDNL 1128
            + IK QA +W IIYGFADSLVLRCAVE+GI DII NN+  + LA LA+KLP+++V+ D L
Sbjct: 15   VSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYL 74

Query: 1127 YRILRYLVKMEILRVEKSDDG-QKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWH 951
            YR++RYLV + I+  E  + G +K Y+L+P+ TLL R+A+RSMVPMILGMTQKDFM  WH
Sbjct: 75   YRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWH 134

Query: 950  SMKDGLSDNGT-AFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQ 774
             MK+GL +  T AFEK MGM IW+YLEG+PDQSQLFNEGMAGETRLLT +LI   RD FQ
Sbjct: 135  FMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQ 194

Query: 773  GIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFKSVPSA 594
            G+DSLVD+GGGNGTT+KAI +AFPHIKCTL+DLPHV+ANS+DLPNIE++ GDMFKSVPSA
Sbjct: 195  GLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSA 254

Query: 593  QAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDID 414
            QAI+LKLILHDW DE+ + ILK+C+ A+PK+ GKVIIVDVAL+EES+HEL+ TRLILDID
Sbjct: 255  QAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDID 314

Query: 413  MLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 285
            MLVNTGG+ERT + WE ++K AGF   KIR I AIQSVIE FP
Sbjct: 315  MLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357