BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00002716
         (1876 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277399.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   505   e-140
ref|XP_002270570.2| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   502   e-139
ref|XP_003540274.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   483   e-134
ref|XP_003543397.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   483   e-134
gb|ACU19018.1| unknown [Glycine max]                                  482   e-133

>ref|XP_002277399.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A [Vitis vinifera]
            gi|296084011|emb|CBI24399.3| unnamed protein product
            [Vitis vinifera]
          Length = 393

 Score =  505 bits (1300), Expect = e-140
 Identities = 260/379 (68%), Positives = 293/379 (77%), Gaps = 2/379 (0%)
 Frame = -1

Query: 1690 MEASGMDEGKMKENHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVTCCKHEFHLQ 1511
            MEA GM+E    E HLTSAAAFVEGGIQ+ACDDACSICLEAFCDSDPSTVT CKHEFHLQ
Sbjct: 1    MEAPGMEESNKSECHLTSAAAFVEGGIQEACDDACSICLEAFCDSDPSTVTSCKHEFHLQ 60

Query: 1510 CILEWCQRSSQCPMCWQPISLKDPTCQELLDAVEHERKLRFNPPRNATIFHHPALGEFEL 1331
            CILEWCQRSSQCPMCWQPISLKDPT QELL+AVE ER  RFNP RNATIFHHP LG+FEL
Sbjct: 61   CILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERTFRFNPSRNATIFHHPTLGDFEL 120

Query: 1330 QHLPVGASDSELEERIIQHL-AAAAMGRSHHMARREGQRSRSTAQGRPQFLVFSANPNAS 1154
            QHLPVGA+D+ELEERIIQHL AAAAMGR+ H+ARREGQR+RS+AQGRPQFLVFSA+P+  
Sbjct: 121  QHLPVGANDAELEERIIQHLAAAAAMGRARHIARREGQRTRSSAQGRPQFLVFSAHPSTP 180

Query: 1153 PTSPISSPPDQGGRNEPAXXXXXXXXXXXXXPEREETSQGIPQLLHAHTEPSPAPASGSS 974
               P SS P Q G +EPA                EE+S+     L +  E   A ASGS 
Sbjct: 181  QAGPASSSPAQRGEDEPAPAITAAMPSSLPTAVGEESSRLTTSQLSSQAEEITASASGSI 240

Query: 973  VLASNQSRFSINNRNSASQSSPINQDRAGPSELQSISESLKSRFSAVSTRYKESITKNTR 794
            +  +NQ   S NNR S  QS P  QD+AGPSE QS SE+LKSRF+AVS RYKESI+K+TR
Sbjct: 241  IHTTNQHGTSSNNRRSPGQSLPSGQDKAGPSEFQSFSETLKSRFNAVSMRYKESISKSTR 300

Query: 793  GWKERLFSRNTSMSDLGSEVRREVNAGIASVSRLMERLDTRENSRVTGSSGSDNLVD-SA 617
            GWKERLFSRNTSM+D+GS+VRREVNAGIA+VSR+ME L+TR+NSR T SS SD+  D S+
Sbjct: 301  GWKERLFSRNTSMADIGSDVRREVNAGIATVSRMMEHLETRDNSRTTPSSVSDSSEDTSS 360

Query: 616  TEPDNQRSTETHVRNPLND 560
                +QR  ET   NPL D
Sbjct: 361  PSLGDQRMAETSAHNPLGD 379


>ref|XP_002270570.2| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Vitis vinifera]
          Length = 387

 Score =  502 bits (1292), Expect = e-139
 Identities = 264/392 (67%), Positives = 295/392 (75%), Gaps = 1/392 (0%)
 Frame = -1

Query: 1690 MEASGMDEGKMKENHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVTCCKHEFHLQ 1511
            ME  GM+E K  ENH+TSAAAFVEGG+Q+ACDDACSICLEAFCDSDPST+T CKHEFHLQ
Sbjct: 1    MEVEGMEEVKNSENHMTSAAAFVEGGVQEACDDACSICLEAFCDSDPSTLTSCKHEFHLQ 60

Query: 1510 CILEWCQRSSQCPMCWQPISLKDPTCQELLDAVEHERKLRFNPPRNATIFHHPALGEFEL 1331
            CILEWCQRSSQCPMCWQ ISLKDPT QELL+AVE ER +R  PPR+A IFHHP LG+FEL
Sbjct: 61   CILEWCQRSSQCPMCWQSISLKDPTSQELLEAVERERNIRLTPPRSA-IFHHPTLGDFEL 119

Query: 1330 QHLPVGASDSELEERIIQHL-AAAAMGRSHHMARREGQRSRSTAQGRPQFLVFSANPNAS 1154
            QHLPVG +D ELEERIIQHL AAAAMGR+HH+ARREGQRSRS+A GR  FLVFS +PNA+
Sbjct: 120  QHLPVGTNDPELEERIIQHLAAAAAMGRAHHIARREGQRSRSSAHGRSHFLVFSTHPNAT 179

Query: 1153 PTSPISSPPDQGGRNEPAXXXXXXXXXXXXXPEREETSQGIPQLLHAHTEPSPAPASGSS 974
             + P+ +   Q G NEPA                +E SQ IPQ     T+   A AS S+
Sbjct: 180  ASGPVPASLVQVGENEPAAITVASPSIPLPAG-GDEPSQEIPQFPSVQTDHMSASASRSA 238

Query: 973  VLASNQSRFSINNRNSASQSSPINQDRAGPSELQSISESLKSRFSAVSTRYKESITKNTR 794
            V      R S NNR+SASQSSP +QDRAGPSELQS SESLKSR +AVS RYKESI+K+TR
Sbjct: 239  V---TPRRISFNNRSSASQSSPPDQDRAGPSELQSFSESLKSRLNAVSMRYKESISKSTR 295

Query: 793  GWKERLFSRNTSMSDLGSEVRREVNAGIASVSRLMERLDTRENSRVTGSSGSDNLVDSAT 614
            GWKERLFSRN SM+DLGSEVRREVNAGIASVSR+MERLDTREN+    +S S +L DS T
Sbjct: 296  GWKERLFSRNNSMADLGSEVRREVNAGIASVSRMMERLDTRENNGAGQASVSSHLADSVT 355

Query: 613  EPDNQRSTETHVRNPLNDXXXXXXXXXXSGQN 518
            E  NQ   ET   NPLND          SG N
Sbjct: 356  EQSNQNERETRAENPLNDSNTPAACAASSGSN 387


>ref|XP_003540274.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Glycine max]
          Length = 401

 Score =  483 bits (1244), Expect = e-134
 Identities = 248/373 (66%), Positives = 284/373 (76%), Gaps = 10/373 (2%)
 Frame = -1

Query: 1690 MEASGMDEGKM---------KENHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT 1538
            ME +GM+E  +          E HLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT
Sbjct: 1    MEGAGMEENNIINDDDNNNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT 60

Query: 1537 CCKHEFHLQCILEWCQRSSQCPMCWQPISLKDPTCQELLDAVEHERKLRFNPPRNATIFH 1358
             CKHEFHLQCILEWCQRSSQCPMCWQPISLKDPT QELL+AVE ER  RFNP RNATIFH
Sbjct: 61   SCKHEFHLQCILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFH 120

Query: 1357 HPALGEFELQHLPVGASDSELEERIIQHL-AAAAMGRSHHMARREGQRSRSTAQGRPQFL 1181
            HP LG+FELQHLPVGASD++LEERIIQHL AAAAMGR+ H+ARREGQR+RS+AQGRP FL
Sbjct: 121  HPTLGDFELQHLPVGASDADLEERIIQHLAAAAAMGRARHIARREGQRNRSSAQGRPHFL 180

Query: 1180 VFSANPNASPTSPISSPPDQGGRNEPAXXXXXXXXXXXXXPEREETSQGIPQLLHAHTEP 1001
            VFS NPN+ P +P SS P Q G +EP                 E +   +   + A  E 
Sbjct: 181  VFSTNPNSPPMAPASSSPSQRGDDEPIPTIAVANLSPTPATGEESSQLTLVPPIQA--EQ 238

Query: 1000 SPAPASGSSVLASNQSRFSINNRNSASQSSPINQDRAGPSELQSISESLKSRFSAVSTRY 821
              A  SGS+ LA++    S NNR S +QSSP +QDRAGPSELQS SESLKS+ +AVSTRY
Sbjct: 239  VSASGSGSTALATDHQGSSYNNRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRY 298

Query: 820  KESITKNTRGWKERLFSRNTSMSDLGSEVRREVNAGIASVSRLMERLDTRENSRVTGSSG 641
            KESI+K+TRGWKER FSRN +MSDLGSEVRREVNAGIA+VSR+MERL+T +N+R   +S 
Sbjct: 299  KESISKSTRGWKERWFSRNNTMSDLGSEVRREVNAGIATVSRMMERLETSDNNRSNSNSA 358

Query: 640  SDNLVDSATEPDN 602
              N+ D + +  N
Sbjct: 359  PSNIEDGSVQEYN 371


>ref|XP_003543397.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Glycine max]
          Length = 398

 Score =  483 bits (1244), Expect = e-134
 Identities = 250/374 (66%), Positives = 289/374 (77%), Gaps = 8/374 (2%)
 Frame = -1

Query: 1690 MEASGMDEGKM------KENHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVTCCK 1529
            ME +GM+E  +       E HLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT CK
Sbjct: 1    MEGAGMEENNIINDDNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVTSCK 60

Query: 1528 HEFHLQCILEWCQRSSQCPMCWQPISLKDPTCQELLDAVEHERKLRFNPPRNATIFHHPA 1349
            HEFHLQCILEWCQRSSQCPMCWQPISLKDPT QELL+AVE ER  RFNP RNATIFHHP 
Sbjct: 61   HEFHLQCILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFHHPT 120

Query: 1348 LGEFELQHLPVGASDSELEERIIQHL-AAAAMGRSHHMARREGQRSRSTAQGRPQFLVFS 1172
            LG+FELQHLPVGASD++LEERIIQHL AAAAMGR+ H+ARREGQR+RS+AQGR  FLVFS
Sbjct: 121  LGDFELQHLPVGASDADLEERIIQHLAAAAAMGRARHVARREGQRNRSSAQGRQHFLVFS 180

Query: 1171 ANPNASPTSPISSPPDQGGRNEPAXXXXXXXXXXXXXPEREETSQGIPQLLH-AHTEPSP 995
             +PN+ P +P SS P Q G +EP                 EE+SQ  P L+     E   
Sbjct: 181  THPNSPPMAPASSSPSQRGDDEPT-PTIDVANLSPTPATGEESSQ--PTLVSPVQAEQVS 237

Query: 994  APASGSSVLASNQSRFSINNRNSASQSSPINQDRAGPSELQSISESLKSRFSAVSTRYKE 815
            A  SG++ LA++    S NNR S +QSSP +QDRAGPSELQS SESLKS+ +AVSTRYKE
Sbjct: 238  ASGSGTTALATDHQGSSYNNRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRYKE 297

Query: 814  SITKNTRGWKERLFSRNTSMSDLGSEVRREVNAGIASVSRLMERLDTRENSRVTGSSGSD 635
            SI+K+TRGWKER FSRN +MSDLGSEVRREVNAGIA+VSR+MERL+TR+N+R   +S   
Sbjct: 298  SISKSTRGWKERWFSRNNTMSDLGSEVRREVNAGIATVSRMMERLETRDNNRSNSNSAPS 357

Query: 634  NLVDSATEPDNQRS 593
            N+ D + +  N ++
Sbjct: 358  NIEDGSVQGSNDQT 371


>gb|ACU19018.1| unknown [Glycine max]
          Length = 401

 Score =  482 bits (1240), Expect = e-133
 Identities = 247/373 (66%), Positives = 283/373 (75%), Gaps = 10/373 (2%)
 Frame = -1

Query: 1690 MEASGMDEGKM---------KENHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT 1538
            ME +GM+E  +          E HLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT
Sbjct: 1    MEGAGMEENNIINDDDNNNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT 60

Query: 1537 CCKHEFHLQCILEWCQRSSQCPMCWQPISLKDPTCQELLDAVEHERKLRFNPPRNATIFH 1358
             CKHEFHLQCILEWCQRSSQCPMCWQPISLKDPT QELL+AVE ER  RFNP RNATIFH
Sbjct: 61   SCKHEFHLQCILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFH 120

Query: 1357 HPALGEFELQHLPVGASDSELEERIIQHL-AAAAMGRSHHMARREGQRSRSTAQGRPQFL 1181
            HP LG+FELQHLPVGASD++LEERIIQHL AAAAMGR+ H+ARREGQR+RS+AQGRP FL
Sbjct: 121  HPTLGDFELQHLPVGASDADLEERIIQHLAAAAAMGRARHIARREGQRNRSSAQGRPHFL 180

Query: 1180 VFSANPNASPTSPISSPPDQGGRNEPAXXXXXXXXXXXXXPEREETSQGIPQLLHAHTEP 1001
             FS NPN+ P +P SS P Q G +EP                 E +   +   + A  E 
Sbjct: 181  AFSTNPNSPPMAPASSSPSQRGDDEPIPTIAVANLSPTPATGEESSQLTLVPPIQA--EQ 238

Query: 1000 SPAPASGSSVLASNQSRFSINNRNSASQSSPINQDRAGPSELQSISESLKSRFSAVSTRY 821
              A  SGS+ LA++    S NNR S +QSSP +QDRAGPSELQS SESLKS+ +AVSTRY
Sbjct: 239  VSASGSGSTALATDHQGSSYNNRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRY 298

Query: 820  KESITKNTRGWKERLFSRNTSMSDLGSEVRREVNAGIASVSRLMERLDTRENSRVTGSSG 641
            KESI+K+TRGWKER FSRN +MSDLGSEVRREVNAGIA+VSR+MERL+T +N+R   +S 
Sbjct: 299  KESISKSTRGWKERWFSRNNTMSDLGSEVRREVNAGIATVSRMMERLETSDNNRSNSNSA 358

Query: 640  SDNLVDSATEPDN 602
              N+ D + +  N
Sbjct: 359  PSNIEDGSVQEYN 371