BLASTX nr result
ID: Coptis21_contig00002560
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00002560
(1870 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast prec... 843 0.0
ref|XP_002304493.1| predicted protein [Populus trichocarpa] gi|2... 838 0.0
gb|AFF18844.2| gamma-glutamylcysteine synthetase [Dimocarpus lon... 837 0.0
ref|XP_002298035.1| predicted protein [Populus trichocarpa] gi|2... 836 0.0
gb|ABK96626.1| unknown [Populus trichocarpa x Populus deltoides] 834 0.0
>ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast precursor, putative [Ricinus
communis] gi|223549699|gb|EEF51187.1| Glutamate--cysteine
ligase, chloroplast precursor, putative [Ricinus
communis]
Length = 526
Score = 843 bits (2178), Expect = 0.0
Identities = 403/458 (87%), Positives = 428/458 (93%)
Frame = +1
Query: 274 SKRGNRFLVAASPPTEDAVIATEPLTKQDLVNYLASGCKPKEQWRIGTEHEKFGFELDTL 453
S+RGN+ +VAASPP EDAVIATEPLTK+DLV YLASGCKPKE+WRIGTEHEKFGFEL TL
Sbjct: 69 SRRGNQMIVAASPPIEDAVIATEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFELGTL 128
Query: 454 RPMKYEQIADLLYGLAERFDWEKIMEGDYIIGLKQGKQSVSLEPGGQFELSGAPLETLHQ 633
RPMKYEQIA+LL G+AERFDWEK+MEGDYIIGLKQGKQS+SLEPGGQFELSGAPLETLHQ
Sbjct: 129 RPMKYEQIAELLNGIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQ 188
Query: 634 TCAEVNSHLYQVKAVAEEXXXXXXXXXXQPKWRIKDIPIMPKGRYDIMRNYMPKVGSLGL 813
TCAEVNSHLYQVKAV EE QPKW +KDIPIMPKGRY+IMRNYMPKVG+LGL
Sbjct: 189 TCAEVNSHLYQVKAVTEEMGIGFLGIGFQPKWGVKDIPIMPKGRYEIMRNYMPKVGTLGL 248
Query: 814 DMMFRTCTVQVNLDFSSESDMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIW 993
DMMFRTCTVQVNLDFSSE+DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRS IW
Sbjct: 249 DMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQIW 308
Query: 994 SDTDNNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRNKKYIDCSGLSFRDFLEGKLPA 1173
+DTD +RTGMLPFVFDDSFGFEQYVDYALDVPMYFVYR KKYIDC+G++FRDFL GKLP
Sbjct: 309 TDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPC 368
Query: 1174 APGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQN 1353
PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDE+SLQN
Sbjct: 369 IPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEISLQN 428
Query: 1354 VLDMTADWTSAERQMLRNKVPVTGLKTPFRDGLLRHVAEDVLKLAKDGLERRGYKETGFL 1533
VLDMTADWT ER+MLRNKVP TGLKTPFRDGLL+HVAEDVLKLAKDGLERRGYKE GFL
Sbjct: 429 VLDMTADWTPEEREMLRNKVPKTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEIGFL 488
Query: 1534 NALTEVVTTGVTPAEKLLELYEGKWGRSVDPVFEELLY 1647
N +TEVV TGVTPAE+LLELY GKWG+SVDPVFEELLY
Sbjct: 489 NEVTEVVRTGVTPAERLLELYNGKWGQSVDPVFEELLY 526
>ref|XP_002304493.1| predicted protein [Populus trichocarpa] gi|222841925|gb|EEE79472.1|
predicted protein [Populus trichocarpa]
Length = 526
Score = 838 bits (2164), Expect = 0.0
Identities = 411/528 (77%), Positives = 454/528 (85%), Gaps = 30/528 (5%)
Frame = +1
Query: 154 MALISKIGCSGYYLGSEIIQCNKNVQNILVQEKTRLP--------------SCSS----- 276
M L+S+ G S Y + ++I +C K QN+++ + + SC+S
Sbjct: 1 MTLVSQAGPS-YCIPTDISRC-KTAQNVVIGMASNIDASKLKETPARFSSLSCNSSKTPK 58
Query: 277 -----------KRGNRFLVAASPPTEDAVIATEPLTKQDLVNYLASGCKPKEQWRIGTEH 423
KRG++F+VAASPPTEDAV+ATEPLTK+DL+ YLASGCKPKE WRIGTEH
Sbjct: 59 ILSSECLSVGMKRGHQFVVAASPPTEDAVVATEPLTKEDLIRYLASGCKPKENWRIGTEH 118
Query: 424 EKFGFELDTLRPMKYEQIADLLYGLAERFDWEKIMEGDYIIGLKQGKQSVSLEPGGQFEL 603
EKFGFE+ TLRPMKYEQIA+LL+G+AERFDW+KIMEGD IIGLKQ KQS+SLEPGGQFEL
Sbjct: 119 EKFGFEIGTLRPMKYEQIAELLHGIAERFDWDKIMEGDTIIGLKQEKQSISLEPGGQFEL 178
Query: 604 SGAPLETLHQTCAEVNSHLYQVKAVAEEXXXXXXXXXXQPKWRIKDIPIMPKGRYDIMRN 783
SGAP+ETLHQTCAEVNSHLYQVKAVAEE QPKW +KDIPIMPKGRYDIMRN
Sbjct: 179 SGAPVETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWVLKDIPIMPKGRYDIMRN 238
Query: 784 YMPKVGSLGLDMMFRTCTVQVNLDFSSESDMIRKFRAGLALQPIATALFANSPFTEGKPN 963
YMPKVGSLGLDMMFRTCTVQVNLDFSSE+DMIRKFRAGLALQPIATALFANSPFT+GKPN
Sbjct: 239 YMPKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTDGKPN 298
Query: 964 GYLSMRSHIWSDTDNNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRNKKYIDCSGLSF 1143
GYLS RSHIW+DTDNNR GMLPFVFDD+FGFEQYVDYALDVPMYFVYR +KYIDC+G+SF
Sbjct: 299 GYLSKRSHIWTDTDNNRAGMLPFVFDDNFGFEQYVDYALDVPMYFVYRKEKYIDCTGMSF 358
Query: 1144 RDFLEGKLPAAPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG 1323
RDFL G+LP PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG
Sbjct: 359 RDFLAGRLPCIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG 418
Query: 1324 ILYDEVSLQNVLDMTADWTSAERQMLRNKVPVTGLKTPFRDGLLRHVAEDVLKLAKDGLE 1503
+LYDE SLQ+VLDMTADWTS ERQMLRNKVP TGLKTPFRDGLLRHVAE+VLKLAKDGLE
Sbjct: 419 LLYDEDSLQSVLDMTADWTSEERQMLRNKVPKTGLKTPFRDGLLRHVAEEVLKLAKDGLE 478
Query: 1504 RRGYKETGFLNALTEVVTTGVTPAEKLLELYEGKWGRSVDPVFEELLY 1647
RRG+KE GFLNA+ EV +TG+TPAEKLLELY GKWG+S+DPVFEELLY
Sbjct: 479 RRGFKEVGFLNAVAEVASTGITPAEKLLELYHGKWGQSIDPVFEELLY 526
>gb|AFF18844.2| gamma-glutamylcysteine synthetase [Dimocarpus longan]
Length = 511
Score = 837 bits (2162), Expect = 0.0
Identities = 413/512 (80%), Positives = 449/512 (87%), Gaps = 14/512 (2%)
Frame = +1
Query: 154 MALISKIGCSGYYLGSEIIQCNKNVQNIL--VQEKTRLPSC------------SSKRGNR 291
MAL+S+ G + Y + SEI ++ V ++ V+ SC S+RG R
Sbjct: 1 MALVSQGGAT-YRVRSEITPGHRGVFGLVNNVEASRVKESCVSFSSLSCYSTKGSRRGYR 59
Query: 292 FLVAASPPTEDAVIATEPLTKQDLVNYLASGCKPKEQWRIGTEHEKFGFELDTLRPMKYE 471
+VAASPPT+DAVIA EPLTK+DLV YLASGCKP+E+WRIGTEHEKFGFE+ TLRPMKYE
Sbjct: 60 AIVAASPPTDDAVIAAEPLTKEDLVGYLASGCKPREKWRIGTEHEKFGFEIGTLRPMKYE 119
Query: 472 QIADLLYGLAERFDWEKIMEGDYIIGLKQGKQSVSLEPGGQFELSGAPLETLHQTCAEVN 651
QIA+LL +AERFDWEKIME + IIGLKQGKQS+SLEPGGQFELSGAPLETLHQTCAEVN
Sbjct: 120 QIAELLNSIAERFDWEKIMEDNNIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVN 179
Query: 652 SHLYQVKAVAEEXXXXXXXXXXQPKWRIKDIPIMPKGRYDIMRNYMPKVGSLGLDMMFRT 831
SHLYQVKAVAEE QPKW IKDIP+MPKGRY+IM+NYMPKVGSLGLDMMFRT
Sbjct: 180 SHLYQVKAVAEEMGIGFLGIGFQPKWGIKDIPVMPKGRYEIMKNYMPKVGSLGLDMMFRT 239
Query: 832 CTVQVNLDFSSESDMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWSDTDNN 1011
CTVQVNLDFSSE+DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRS IW+DTDNN
Sbjct: 240 CTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQIWTDTDNN 299
Query: 1012 RTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRNKKYIDCSGLSFRDFLEGKLPAAPGELP 1191
RTGMLPFVFDDSFGFEQYVDYALDVPMYFVYR KKYIDC+G+SFRDFL GKLP PGELP
Sbjct: 300 RTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMSFRDFLVGKLPCLPGELP 359
Query: 1192 TLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQNVLDMTA 1371
TLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDEVSLQN LD+TA
Sbjct: 360 TLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEVSLQNALDLTA 419
Query: 1372 DWTSAERQMLRNKVPVTGLKTPFRDGLLRHVAEDVLKLAKDGLERRGYKETGFLNALTEV 1551
DWTS ERQMLRNKVP TGLKTPFRDGLLRHVA+D+LKLAKDGLERRG+KE+GFLNA+ EV
Sbjct: 420 DWTSEERQMLRNKVPKTGLKTPFRDGLLRHVAQDILKLAKDGLERRGFKESGFLNAVAEV 479
Query: 1552 VTTGVTPAEKLLELYEGKWGRSVDPVFEELLY 1647
V TGVTPAEKLLE+Y GKWG+SVDPVFEELLY
Sbjct: 480 VRTGVTPAEKLLEMYHGKWGQSVDPVFEELLY 511
>ref|XP_002298035.1| predicted protein [Populus trichocarpa] gi|222845293|gb|EEE82840.1|
predicted protein [Populus trichocarpa]
Length = 526
Score = 836 bits (2159), Expect = 0.0
Identities = 403/481 (83%), Positives = 436/481 (90%), Gaps = 1/481 (0%)
Frame = +1
Query: 208 IQCNKN-VQNILVQEKTRLPSCSSKRGNRFLVAASPPTEDAVIATEPLTKQDLVNYLASG 384
+ CN + IL E S KRG+R +VAASPPTEDAVIATEPLTK+DL+ YLASG
Sbjct: 49 LSCNSSKTPQILSSESL---SVGMKRGHRVVVAASPPTEDAVIATEPLTKEDLIGYLASG 105
Query: 385 CKPKEQWRIGTEHEKFGFELDTLRPMKYEQIADLLYGLAERFDWEKIMEGDYIIGLKQGK 564
CKPKE WRIGTEHEKFGFE+ TLRPMKYEQIA LL+G+AERFDW++IMEGD IIGL+QGK
Sbjct: 106 CKPKENWRIGTEHEKFGFEIGTLRPMKYEQIAGLLHGIAERFDWDRIMEGDNIIGLQQGK 165
Query: 565 QSVSLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEXXXXXXXXXXQPKWRIKDI 744
QS+SLEPGGQFELSGAP+ETLHQTCAEVNSHLYQVKAVAEE QPKW +KDI
Sbjct: 166 QSISLEPGGQFELSGAPVETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGLKDI 225
Query: 745 PIMPKGRYDIMRNYMPKVGSLGLDMMFRTCTVQVNLDFSSESDMIRKFRAGLALQPIATA 924
P+MPKGRYDIMRNYMPKVGSLGLDMMFRTCTVQVNLDF SE+DMIRKFRAGLALQPIATA
Sbjct: 226 PVMPKGRYDIMRNYMPKVGSLGLDMMFRTCTVQVNLDFISEADMIRKFRAGLALQPIATA 285
Query: 925 LFANSPFTEGKPNGYLSMRSHIWSDTDNNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVY 1104
LFANSPFTEGKPNGYLS+RSHIW+DTDNNR GMLPFVF+D+FGFEQYVDYALDVPMYFVY
Sbjct: 286 LFANSPFTEGKPNGYLSLRSHIWTDTDNNRAGMLPFVFNDTFGFEQYVDYALDVPMYFVY 345
Query: 1105 RNKKYIDCSGLSFRDFLEGKLPAAPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 1284
R KKYIDC+G+SFRDFL GKLP PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP
Sbjct: 346 RKKKYIDCTGMSFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 405
Query: 1285 WRRLCALPAFWVGILYDEVSLQNVLDMTADWTSAERQMLRNKVPVTGLKTPFRDGLLRHV 1464
WRRLCALPAFWVG+LYDE SLQ+VLDMTADWTS ERQMLRNKVP TGLKTPFR GLLRHV
Sbjct: 406 WRRLCALPAFWVGLLYDEDSLQSVLDMTADWTSEERQMLRNKVPKTGLKTPFRGGLLRHV 465
Query: 1465 AEDVLKLAKDGLERRGYKETGFLNALTEVVTTGVTPAEKLLELYEGKWGRSVDPVFEELL 1644
AE+VLKLAKDGLERRG+KE GFLNA+++VV+TG+TPAEKLLELY GKWGRS+DPVFEELL
Sbjct: 466 AEEVLKLAKDGLERRGFKEVGFLNAVSDVVSTGITPAEKLLELYHGKWGRSIDPVFEELL 525
Query: 1645 Y 1647
Y
Sbjct: 526 Y 526
>gb|ABK96626.1| unknown [Populus trichocarpa x Populus deltoides]
Length = 526
Score = 834 bits (2154), Expect = 0.0
Identities = 409/528 (77%), Positives = 452/528 (85%), Gaps = 30/528 (5%)
Frame = +1
Query: 154 MALISKIGCSGYYLGSEIIQCNKNVQNILVQEKTRLP--------------SCSS----- 276
M L+S+ G S Y + ++I +C K QN+++ + + SC+S
Sbjct: 1 MTLVSQAGPS-YCIPTDISRC-KTAQNVVIGMASNIDASKLKETPARFSSLSCNSSKTPK 58
Query: 277 -----------KRGNRFLVAASPPTEDAVIATEPLTKQDLVNYLASGCKPKEQWRIGTEH 423
KRG++F+VAASPPTEDAV+ATEPLTK+DL+ YLASGCKPKE WRIGTEH
Sbjct: 59 ILSSECLSVGMKRGHQFVVAASPPTEDAVVATEPLTKEDLIRYLASGCKPKENWRIGTEH 118
Query: 424 EKFGFELDTLRPMKYEQIADLLYGLAERFDWEKIMEGDYIIGLKQGKQSVSLEPGGQFEL 603
EKFGFE+ TLRPMKYEQIA+LL+G+AERFDW+KIMEGD IIGLKQ KQS+SLEPGGQFEL
Sbjct: 119 EKFGFEIGTLRPMKYEQIAELLHGIAERFDWDKIMEGDTIIGLKQEKQSISLEPGGQFEL 178
Query: 604 SGAPLETLHQTCAEVNSHLYQVKAVAEEXXXXXXXXXXQPKWRIKDIPIMPKGRYDIMRN 783
SGAP+ETLHQTCAEVNSHLYQVKAVAEE QPKW +KDIPIMPKGRYDIMRN
Sbjct: 179 SGAPVETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWVLKDIPIMPKGRYDIMRN 238
Query: 784 YMPKVGSLGLDMMFRTCTVQVNLDFSSESDMIRKFRAGLALQPIATALFANSPFTEGKPN 963
YMPKVGSLGLDMMFRTCTVQVNLDFSSE+DMIRKFRAGLALQPIATALFANSPFT+GKPN
Sbjct: 239 YMPKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTDGKPN 298
Query: 964 GYLSMRSHIWSDTDNNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRNKKYIDCSGLSF 1143
GYLS RSHIW+DTDNNR G LPFVFDD+FGFEQYVDYALDVPMYFVYR +KYIDC+G+SF
Sbjct: 299 GYLSKRSHIWTDTDNNRAGKLPFVFDDNFGFEQYVDYALDVPMYFVYRKEKYIDCTGMSF 358
Query: 1144 RDFLEGKLPAAPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG 1323
RDFL G+LP PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG
Sbjct: 359 RDFLAGRLPCIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG 418
Query: 1324 ILYDEVSLQNVLDMTADWTSAERQMLRNKVPVTGLKTPFRDGLLRHVAEDVLKLAKDGLE 1503
+LYDE SLQ+VLDMTADWTS ERQMLRNKVP TGLKTPFRDGLLRHVAE+VLKLAKDGLE
Sbjct: 419 LLYDEDSLQSVLDMTADWTSEERQMLRNKVPKTGLKTPFRDGLLRHVAEEVLKLAKDGLE 478
Query: 1504 RRGYKETGFLNALTEVVTTGVTPAEKLLELYEGKWGRSVDPVFEELLY 1647
RRG+KE GFLNA+ EV +TG+TPAEKLLELY GKWG+S+DP FEELLY
Sbjct: 479 RRGFKEVGFLNAVAEVASTGITPAEKLLELYHGKWGQSIDPAFEELLY 526