BLASTX nr result
ID: Coptis21_contig00002526
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00002526
(1689 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002322806.1| iaa-amino acid hydrolase 4 [Populus trichoca... 348 e-157
gb|ACZ56437.1| IAA-amino acid hydrolase [Populus tomentosa] 349 e-156
ref|XP_002275866.2| PREDICTED: IAA-amino acid hydrolase ILR1-lik... 315 e-145
emb|CBI30348.3| unnamed protein product [Vitis vinifera] 309 e-143
ref|XP_004136498.1| PREDICTED: IAA-amino acid hydrolase ILR1-lik... 317 e-143
>ref|XP_002322806.1| iaa-amino acid hydrolase 4 [Populus trichocarpa]
gi|222867436|gb|EEF04567.1| iaa-amino acid hydrolase 4
[Populus trichocarpa]
Length = 478
Score = 348 bits (893), Expect(2) = e-157
Identities = 173/270 (64%), Positives = 205/270 (75%)
Frame = -2
Query: 1508 FLCHVLLMTSLFFKSWALNNKLDSELDVLTRDLLESGREPEFFDWMKKVRRRIHEYPELA 1329
+LC + ++ S +WAL+ + +S+L LTR+LLES REPEFF W+K++RRRIHE PELA
Sbjct: 51 WLC-LFMILSTCQTAWALDTRSESKLGYLTRELLESAREPEFFGWLKRIRRRIHEDPELA 109
Query: 1328 FEEHKTSQLIRSELDSLGIEYAWPVAKTGVVGSIGSGQYPWFSLRADMDALPMQEMVEWE 1149
FEE+ TSQLIRSELDSLGIEY WP AKTGVVGSIGSG PWF LRADMDALP+QEMVEWE
Sbjct: 110 FEEYNTSQLIRSELDSLGIEYKWPFAKTGVVGSIGSGLQPWFGLRADMDALPIQEMVEWE 169
Query: 1148 HKSKNNGKMHACGHDVHVTMXXXXXXXXXXXXXXXXGTVKLVFQPGEEEGSGAYNMLQEG 969
HKSKNNGKMHACGHD HVTM GTVKLVFQPGEE GAY+ML+EG
Sbjct: 170 HKSKNNGKMHACGHDAHVTMLLGAAKLLERMKDELKGTVKLVFQPGEESYGGAYHMLKEG 229
Query: 968 VLNDIPAMFGLHVWPSLPTGTVASKPGPLFAGSGDFSVIIRGIGGHAARPYRTTDPTIAA 789
L++ +FGLHV P +P GTV S+PGP+ A SG F I+G GGHAARP T DP +AA
Sbjct: 230 ALDNFQGIFGLHVAPEIPVGTVDSRPGPMLAASGRFIATIKGKGGHAARPQDTRDPVVAA 289
Query: 788 ACAILSIQQIVSRETDPLESRVNFISLFQA 699
+ AIL++QQIVSRETDPL++RV + +A
Sbjct: 290 SFAILALQQIVSRETDPLDARVVSVGFVEA 319
Score = 235 bits (599), Expect(2) = e-157
Identities = 116/165 (70%), Positives = 136/165 (82%)
Frame = -1
Query: 627 VVTVGYIQAGEAMNVIPEIVKFGGTFRSMTSEGHSYLKQRIKEIIEAQAHVHRCTAVVDF 448
VV+VG+++AG+A NVIPE V+FGG+ RSMT+EG L+QR+ +I+E QA VH+CTA +DF
Sbjct: 311 VVSVGFVEAGQAGNVIPETVRFGGSIRSMTTEGLVSLQQRVMQIVEMQAAVHQCTASLDF 370
Query: 447 MEGKSIPLPSTVNDEAMYEHAKNVGERLLGESNVHLSPMTMGTEDFSLYSQKMATGFFFI 268
ME K P PSTVNDEAMY+HAK VGE LLGESNV L+PMTMG EDFS YSQKM FFFI
Sbjct: 371 MEEKMRPYPSTVNDEAMYKHAKQVGEALLGESNVLLAPMTMGAEDFSFYSQKMKAAFFFI 430
Query: 267 GIKNETIKSDESLHSPYFRVDEEVLPIGAAFHAAVAISYLGSHAV 133
G KNET+KS + LHSPYF +DEEVL IGAAFHAAVAISYL HA+
Sbjct: 431 GTKNETVKSVKRLHSPYFVIDEEVLSIGAAFHAAVAISYLDGHAI 475
>gb|ACZ56437.1| IAA-amino acid hydrolase [Populus tomentosa]
Length = 430
Score = 349 bits (895), Expect(2) = e-156
Identities = 173/270 (64%), Positives = 205/270 (75%)
Frame = -2
Query: 1508 FLCHVLLMTSLFFKSWALNNKLDSELDVLTRDLLESGREPEFFDWMKKVRRRIHEYPELA 1329
+LC + ++ S +WAL+ + +S+L LTR+LLES REPEFF+W+K++RRRIHE PELA
Sbjct: 3 WLC-LFMILSTCQTTWALDTRSESKLSHLTRELLESAREPEFFEWLKRIRRRIHEDPELA 61
Query: 1328 FEEHKTSQLIRSELDSLGIEYAWPVAKTGVVGSIGSGQYPWFSLRADMDALPMQEMVEWE 1149
FEEH TSQLIRSELDSLGIEY WP AKTGVVGSIGSG PWF LRADMDALP+QEMVEWE
Sbjct: 62 FEEHNTSQLIRSELDSLGIEYKWPFAKTGVVGSIGSGLQPWFGLRADMDALPIQEMVEWE 121
Query: 1148 HKSKNNGKMHACGHDVHVTMXXXXXXXXXXXXXXXXGTVKLVFQPGEEEGSGAYNMLQEG 969
HKSKNNGKMHACGHD HVTM GTVKLVFQPGEE GAY+M++EG
Sbjct: 122 HKSKNNGKMHACGHDAHVTMLLGAAKLLERMKDELKGTVKLVFQPGEESYGGAYHMIKEG 181
Query: 968 VLNDIPAMFGLHVWPSLPTGTVASKPGPLFAGSGDFSVIIRGIGGHAARPYRTTDPTIAA 789
L++ +FGLHV P +P GTV S+PGP+ A SG F I+G GGHAARP T DP +AA
Sbjct: 182 ALDNFQGIFGLHVAPEIPVGTVDSRPGPMLAASGRFIATIKGKGGHAARPQDTRDPVVAA 241
Query: 788 ACAILSIQQIVSRETDPLESRVNFISLFQA 699
+ AIL++QQIVSRETDPL +RV + +A
Sbjct: 242 SFAILALQQIVSRETDPLYARVVSVGFVEA 271
Score = 231 bits (590), Expect(2) = e-156
Identities = 114/165 (69%), Positives = 136/165 (82%)
Frame = -1
Query: 627 VVTVGYIQAGEAMNVIPEIVKFGGTFRSMTSEGHSYLKQRIKEIIEAQAHVHRCTAVVDF 448
VV+VG+++AG+A NVIPE V+FGG+ RS+T+EG L+QR+ +I+E QA VH+CTA +DF
Sbjct: 263 VVSVGFVEAGQAGNVIPETVRFGGSVRSITTEGLVSLQQRVMQIVEMQAAVHQCTASLDF 322
Query: 447 MEGKSIPLPSTVNDEAMYEHAKNVGERLLGESNVHLSPMTMGTEDFSLYSQKMATGFFFI 268
ME K P PSTVNDEAMY+HAK VGE LLGESNV L+PMTMG EDFS YSQKM FFFI
Sbjct: 323 MEEKMRPYPSTVNDEAMYKHAKQVGEALLGESNVLLAPMTMGAEDFSFYSQKMKAAFFFI 382
Query: 267 GIKNETIKSDESLHSPYFRVDEEVLPIGAAFHAAVAISYLGSHAV 133
G KNET+K+ + LHSPYF +DEEVL IGAAFHAAVAISYL HA+
Sbjct: 383 GTKNETVKTVKRLHSPYFVIDEEVLSIGAAFHAAVAISYLDRHAI 427
>ref|XP_002275866.2| PREDICTED: IAA-amino acid hydrolase ILR1-like [Vitis vinifera]
Length = 440
Score = 315 bits (807), Expect(2) = e-145
Identities = 155/267 (58%), Positives = 203/267 (76%), Gaps = 2/267 (0%)
Frame = -2
Query: 1517 MKNFLCHVLLMTSLFFKSWALNNKLDS--ELDVLTRDLLESGREPEFFDWMKKVRRRIHE 1344
M++ ++++ T L+ +WA+ + + E++ L R+LLES RE + +W++ VRR IHE
Sbjct: 1 MESIWWYLMVWTLLYQSTWAVETQTRTGWEMERLGRELLESAREADLLEWIRGVRRSIHE 60
Query: 1343 YPELAFEEHKTSQLIRSELDSLGIEYAWPVAKTGVVGSIGSGQYPWFSLRADMDALPMQE 1164
YPEL FEE++TSQLIR EL+SLGI Y WPVAKTGVV +IGSG P F+LRADMDALP+QE
Sbjct: 61 YPELGFEEYRTSQLIRDELNSLGIRYEWPVAKTGVVATIGSGAQPIFALRADMDALPLQE 120
Query: 1163 MVEWEHKSKNNGKMHACGHDVHVTMXXXXXXXXXXXXXXXXGTVKLVFQPGEEEGSGAYN 984
+VEWEH+SK +GKMHACGHD+HV M GTVKLVFQPGEE +GAY+
Sbjct: 121 LVEWEHRSKIDGKMHACGHDLHVAMLLGAARLLQGKREILKGTVKLVFQPGEEGYAGAYH 180
Query: 983 MLQEGVLNDIPAMFGLHVWPSLPTGTVASKPGPLFAGSGDFSVIIRGIGGHAARPYRTTD 804
MLQ G L++I A+FGLHV PS+ TG +AS+PGP+ AG+G F ++GIGGHAA P++T D
Sbjct: 181 MLQHGALDNINAIFGLHVMPSILTGMIASRPGPMLAGAGLFLATVKGIGGHAAGPHQTRD 240
Query: 803 PTIAAACAILSIQQIVSRETDPLESRV 723
P +AA+ AI+++QQIVSRETDPLE+RV
Sbjct: 241 PILAASLAIVALQQIVSRETDPLEARV 267
Score = 227 bits (578), Expect(2) = e-145
Identities = 112/164 (68%), Positives = 133/164 (81%)
Frame = -1
Query: 627 VVTVGYIQAGEAMNVIPEIVKFGGTFRSMTSEGHSYLKQRIKEIIEAQAHVHRCTAVVDF 448
VVTVG+I+ G+A NVIPE V+FGGT+RS+TS+G SY+++RI+EIIE+QA VHRCTAVV+F
Sbjct: 267 VVTVGFIKGGQAANVIPESVEFGGTYRSLTSQGLSYIQERIQEIIESQAAVHRCTAVVEF 326
Query: 447 MEGKSIPLPSTVNDEAMYEHAKNVGERLLGESNVHLSPMTMGTEDFSLYSQKMATGFFFI 268
E +P P T NDE +YEHAK VGE LLGE NV L P+TMG EDFS YSQK+ F +
Sbjct: 327 REEIPLPYPPTDNDEELYEHAKRVGEILLGEPNVQLVPITMGAEDFSFYSQKVPAVMFEL 386
Query: 267 GIKNETIKSDESLHSPYFRVDEEVLPIGAAFHAAVAISYLGSHA 136
GIKNET+KSD+ LHSPYF +DE LPIGAA HAAVAISYL SHA
Sbjct: 387 GIKNETLKSDQPLHSPYFVIDETALPIGAALHAAVAISYLDSHA 430
>emb|CBI30348.3| unnamed protein product [Vitis vinifera]
Length = 814
Score = 309 bits (792), Expect(2) = e-143
Identities = 152/250 (60%), Positives = 193/250 (77%), Gaps = 2/250 (0%)
Frame = -2
Query: 1466 SWALNNKLDS--ELDVLTRDLLESGREPEFFDWMKKVRRRIHEYPELAFEEHKTSQLIRS 1293
+WA+ + + E++ L R+LLES RE + +W++ VRR IHEYPEL FEE++TSQLIR
Sbjct: 382 TWAVETQTRTGWEMERLGRELLESAREADLLEWIRGVRRSIHEYPELGFEEYRTSQLIRD 441
Query: 1292 ELDSLGIEYAWPVAKTGVVGSIGSGQYPWFSLRADMDALPMQEMVEWEHKSKNNGKMHAC 1113
EL+SLGI Y WPVAKTGVV +IGSG P F+LRADMDALP+QE+VEWEH+SK +GKMHAC
Sbjct: 442 ELNSLGIRYEWPVAKTGVVATIGSGAQPIFALRADMDALPLQELVEWEHRSKIDGKMHAC 501
Query: 1112 GHDVHVTMXXXXXXXXXXXXXXXXGTVKLVFQPGEEEGSGAYNMLQEGVLNDIPAMFGLH 933
GHD+HV M GTVKLVFQPGEE +GAY+MLQ G L++I A+FGLH
Sbjct: 502 GHDLHVAMLLGAARLLQGKREILKGTVKLVFQPGEEGYAGAYHMLQHGALDNINAIFGLH 561
Query: 932 VWPSLPTGTVASKPGPLFAGSGDFSVIIRGIGGHAARPYRTTDPTIAAACAILSIQQIVS 753
V PS+ TG +AS+PGP+ AG+G F ++GIGGHAA P++T DP +AA+ AI+++QQIVS
Sbjct: 562 VMPSILTGMIASRPGPMLAGAGLFLATVKGIGGHAAGPHQTRDPILAASLAIVALQQIVS 621
Query: 752 RETDPLESRV 723
RETDPLE+RV
Sbjct: 622 RETDPLEARV 631
Score = 227 bits (578), Expect(2) = e-143
Identities = 112/164 (68%), Positives = 133/164 (81%)
Frame = -1
Query: 627 VVTVGYIQAGEAMNVIPEIVKFGGTFRSMTSEGHSYLKQRIKEIIEAQAHVHRCTAVVDF 448
VVTVG+I+ G+A NVIPE V+FGGT+RS+TS+G SY+++RI+EIIE+QA VHRCTAVV+F
Sbjct: 631 VVTVGFIKGGQAANVIPESVEFGGTYRSLTSQGLSYIQERIQEIIESQAAVHRCTAVVEF 690
Query: 447 MEGKSIPLPSTVNDEAMYEHAKNVGERLLGESNVHLSPMTMGTEDFSLYSQKMATGFFFI 268
E +P P T NDE +YEHAK VGE LLGE NV L P+TMG EDFS YSQK+ F +
Sbjct: 691 REEIPLPYPPTDNDEELYEHAKRVGEILLGEPNVQLVPITMGAEDFSFYSQKVPAVMFEL 750
Query: 267 GIKNETIKSDESLHSPYFRVDEEVLPIGAAFHAAVAISYLGSHA 136
GIKNET+KSD+ LHSPYF +DE LPIGAA HAAVAISYL SHA
Sbjct: 751 GIKNETLKSDQPLHSPYFVIDETALPIGAALHAAVAISYLDSHA 794
Score = 284 bits (727), Expect(2) = e-132
Identities = 142/222 (63%), Positives = 168/222 (75%)
Frame = -2
Query: 1373 MKKVRRRIHEYPELAFEEHKTSQLIRSELDSLGIEYAWPVAKTGVVGSIGSGQYPWFSLR 1194
M+ VRR+IH+YPEL FEEHKTS+LIR+EL+SLGI Y WPVAKTGVV SIGSG P F+LR
Sbjct: 1 MRGVRRKIHQYPELGFEEHKTSELIRAELNSLGIGYKWPVAKTGVVASIGSGDQPTFALR 60
Query: 1193 ADMDALPMQEMVEWEHKSKNNGKMHACGHDVHVTMXXXXXXXXXXXXXXXXGTVKLVFQP 1014
ADMDALP+QE+VEWE+KSK GKMHACGHD HV M GTVKLVFQP
Sbjct: 61 ADMDALPLQELVEWEYKSKIEGKMHACGHDSHVAMLLGAAKLLQAKRGMLKGTVKLVFQP 120
Query: 1013 GEEEGSGAYNMLQEGVLNDIPAMFGLHVWPSLPTGTVASKPGPLFAGSGDFSVIIRGIGG 834
GEE +GAY+ML+EG L D+ M GLHV P++PTG +AS+ GPL AG G FS I+G GG
Sbjct: 121 GEEGYAGAYHMLKEGALEDVKGMLGLHVIPTVPTGGIASRAGPLLAGVGLFSATIQGKGG 180
Query: 833 HAARPYRTTDPTIAAACAILSIQQIVSRETDPLESRVNFISL 708
H A P+ DP +AA+ AIL++QQIVSRETDPLE+RV + L
Sbjct: 181 HGASPHTAKDPVLAASFAILALQQIVSRETDPLEARVVTVGL 222
Score = 216 bits (549), Expect(2) = e-132
Identities = 104/164 (63%), Positives = 125/164 (76%)
Frame = -1
Query: 627 VVTVGYIQAGEAMNVIPEIVKFGGTFRSMTSEGHSYLKQRIKEIIEAQAHVHRCTAVVDF 448
VVTVG + GEA NVIPE VK GGTFRS+TS+G YL++RIKE+IE QA VH C A VDF
Sbjct: 217 VVTVGLVDGGEAGNVIPESVKIGGTFRSLTSQGLLYLQERIKEVIETQAAVHGCDAAVDF 276
Query: 447 MEGKSIPLPSTVNDEAMYEHAKNVGERLLGESNVHLSPMTMGTEDFSLYSQKMATGFFFI 268
ME + +P P +NDE +YEHAK VGE L+GE NV L P+TMG EDFS Y+++ F +
Sbjct: 277 MEERGMPHPVMINDETLYEHAKKVGEILVGEPNVELLPITMGAEDFSFYTKRFPAAMFTV 336
Query: 267 GIKNETIKSDESLHSPYFRVDEEVLPIGAAFHAAVAISYLGSHA 136
GIKNET+KSD LHSPYF +DE+ P+GAAF+AAVAISYL HA
Sbjct: 337 GIKNETLKSDYPLHSPYFFIDEDAFPVGAAFYAAVAISYLDDHA 380
>ref|XP_004136498.1| PREDICTED: IAA-amino acid hydrolase ILR1-like 7-like [Cucumis
sativus] gi|449525449|ref|XP_004169730.1| PREDICTED:
IAA-amino acid hydrolase ILR1-like 7-like [Cucumis
sativus]
Length = 433
Score = 317 bits (812), Expect(2) = e-143
Identities = 155/265 (58%), Positives = 190/265 (71%)
Frame = -2
Query: 1517 MKNFLCHVLLMTSLFFKSWALNNKLDSELDVLTRDLLESGREPEFFDWMKKVRRRIHEYP 1338
M+ L L F S AL + EL LT +LLES R P+FFDW+ + RR++HE P
Sbjct: 1 MERILVRALFFIFPFLISSALGTEPPLELSHLTLELLESARNPKFFDWLVRARRKLHENP 60
Query: 1337 ELAFEEHKTSQLIRSELDSLGIEYAWPVAKTGVVGSIGSGQYPWFSLRADMDALPMQEMV 1158
EL+FEE +TSQ IR+EL+SLGI + WPVAKTG+V SIGSG +PWF+LRADMDALP+QEMV
Sbjct: 61 ELSFEEFETSQFIRTELESLGINFTWPVAKTGIVASIGSGAHPWFALRADMDALPIQEMV 120
Query: 1157 EWEHKSKNNGKMHACGHDVHVTMXXXXXXXXXXXXXXXXGTVKLVFQPGEEEGSGAYNML 978
EWEHKSK +GKMHACGHD HVTM GTVKLVFQPGEE +GAY+ML
Sbjct: 121 EWEHKSKKDGKMHACGHDAHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRAGAYHML 180
Query: 977 QEGVLNDIPAMFGLHVWPSLPTGTVASKPGPLFAGSGDFSVIIRGIGGHAARPYRTTDPT 798
+EG L+ +FGLH+ P LP GT+ S+ GP AGSG F I+GIGGHAA P++ DP
Sbjct: 181 KEGALDKFQGIFGLHIIPDLPIGTIGSRAGPFMAGSGRFQATIQGIGGHAAWPHKARDPV 240
Query: 797 IAAACAILSIQQIVSRETDPLESRV 723
+A + AI+S+Q I+SRETDPL+SRV
Sbjct: 241 LAMSSAIVSLQHIISRETDPLDSRV 265
Score = 219 bits (557), Expect(2) = e-143
Identities = 109/166 (65%), Positives = 126/166 (75%), Gaps = 1/166 (0%)
Frame = -1
Query: 627 VVTVGYIQAGEAMNVIPEIVKFGGTFRSMTSEGHSYLKQRIKEIIEAQAHVHRCTAVVDF 448
V+TVG+++ G+A NVIPE FGGTFRSMT EG SYL++RI+E+IE QA VH+C A VDF
Sbjct: 265 VITVGFVKGGQAGNVIPETATFGGTFRSMTVEGLSYLQKRIQEVIEVQAAVHQCNATVDF 324
Query: 447 MEGKSIPLPSTVNDEAMYEHAKNVGERLL-GESNVHLSPMTMGTEDFSLYSQKMATGFFF 271
ME K I P+TVNDE +Y HAK VGE LL GESNVH M M EDFS YSQKM FF
Sbjct: 325 MEDKLIFYPATVNDEGLYSHAKKVGEHLLGGESNVHHLSMVMAAEDFSFYSQKMPAAFFM 384
Query: 270 IGIKNETIKSDESLHSPYFRVDEEVLPIGAAFHAAVAISYLGSHAV 133
IG+KNET+KS LHSPY +DE VLP+GAA HAAVAISYL H+V
Sbjct: 385 IGVKNETMKSGTPLHSPYITIDERVLPVGAALHAAVAISYLDEHSV 430