BLASTX nr result
ID: Coptis21_contig00002140
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00002140
(2333 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003552911.1| PREDICTED: taxadiene 5-alpha hydroxylase-lik... 421 e-115
ref|XP_002275049.2| PREDICTED: cytochrome P450 716B1 [Vitis vini... 354 5e-95
emb|CBI25367.3| unnamed protein product [Vitis vinifera] 354 5e-95
emb|CAN70350.1| hypothetical protein VITISV_012582 [Vitis vinifera] 354 5e-95
ref|XP_003537352.1| PREDICTED: LOW QUALITY PROTEIN: taxadiene 5-... 342 3e-91
>ref|XP_003552911.1| PREDICTED: taxadiene 5-alpha hydroxylase-like [Glycine max]
Length = 483
Score = 421 bits (1081), Expect = e-115
Identities = 193/352 (54%), Positives = 265/352 (75%)
Frame = -2
Query: 1405 RYKLIKAAMVRFLKPECLQHYVKDMDDLIAKKLHSETKDRDTVKAVNLMQIVTFDVACTV 1226
RY+L+K M++FLKPECLQ+YVK+MD+L+ L E ++ + ++AV M+ +++++AC +
Sbjct: 126 RYRLVKGEMLKFLKPECLQNYVKEMDELVNATLLREFRENEIIRAVVFMKKLSYEIACNL 185
Query: 1225 LFGIHDETIKNDLLSDFTIAFKAVWSVPINFPGTHFRKGLEARGRIIRRMSPIIMKKKED 1046
LF I DE K L DFT+AFKA+ S+PIN PGT F +G AR RI+ RM PI+ K++E+
Sbjct: 186 LFDIKDEHTKEALFVDFTLAFKAIHSLPINLPGTTFWRGQRARARIVDRMIPILNKRREE 245
Query: 1045 LTKGIVYPKTDVICSLLALTEDTEKPITEQEVMDNFFMLMIATYDTSATLLSLMIMKLAK 866
L+KG++ D++ LLAL ++ +P+ + + DNF L +A++DTSATL+SLMI KL++
Sbjct: 246 LSKGVLSSTNDMLSCLLALRDENHQPLDDDLITDNFIFLFVASHDTSATLMSLMIWKLSR 305
Query: 865 DRKIYEKIREEQLRILSERQGANENLTWNEIQKMKYTWRVAQELMRIIPPVFGSFRKAVK 686
D+++Y K+ EEQ+ I+ +R+G E LTW EIQKMKYTWRVAQELMR+IPP+FGSFRKA+K
Sbjct: 306 DQEVYNKVLEEQMEIIKQREGTEERLTWAEIQKMKYTWRVAQELMRMIPPLFGSFRKALK 365
Query: 685 DTSFGGYDIPKGWQVFWVACGTHLDKDIFDNPTTFDPSRFEHXXXXXXXXXXXPFGAGTR 506
DT++ GYDIPKGWQV+W A GTH++ DIF+NP FDPSRFE+ PFGAG
Sbjct: 366 DTNYKGYDIPKGWQVYWAAYGTHMNDDIFENPHKFDPSRFENPTKPIPPYSYLPFGAGLH 425
Query: 505 MCIGNEFGRIETLVVIHHLVTKFEWSMVDPDEAIKLQPFPHPSLGLPINLKP 350
CIGNEF RIETL +IH+ V +EWS V+P+EAI QP P+PS+GLPI +KP
Sbjct: 426 YCIGNEFARIETLAIIHNFVKMYEWSQVNPEEAITRQPMPYPSMGLPIKIKP 477
>ref|XP_002275049.2| PREDICTED: cytochrome P450 716B1 [Vitis vinifera]
Length = 516
Score = 354 bits (909), Expect = 5e-95
Identities = 163/351 (46%), Positives = 245/351 (69%), Gaps = 1/351 (0%)
Frame = -2
Query: 1405 RYKLIKAAMVRFLKPECLQHYVKDMDDLIAKKLHSETKDRDTVKAVNLMQIVTFDVACTV 1226
R+KL++ A++ FLKPE +Q V MD ++ ++L E + +D+V+ V LM+ +TF V C++
Sbjct: 140 RHKLVRGAIMNFLKPESIQRSVSRMDSVVQQQLFQELEGKDSVQMVGLMKKITFKVTCSL 199
Query: 1225 LFGIHDETIKNDLLSDFTIAFKAVWSVPINFPGTHFRKGLEARGRIIRRMSPIIMKKKED 1046
LFG+ D +LL DFT A K W+VP + PGT FRK L+ARGRI ++++ ++ ++K
Sbjct: 200 LFGLPDGKETEELLEDFTTALKGAWTVPWDLPGTVFRKALQARGRICKQLAELVRERKAK 259
Query: 1045 LTKGIVYPKTDVICSLLALTEDTEKPITEQEVMDNFFMLMIATYDTSATLLSLMIMKLAK 866
+ +G V D+I SL+ L ++ +P++E+E++DN ++IA++DTS LL L+I LA+
Sbjct: 260 IEEGRVDSHEDIISSLITLRQENGQPLSEEEIIDNLISVVIASHDTSTVLLGLLIRHLAR 319
Query: 865 DRKIYEKIREEQLRILSERQG-ANENLTWNEIQKMKYTWRVAQELMRIIPPVFGSFRKAV 689
D ++ +K+ EEQ ++ ++G N LTW E+Q MKYTWRVAQELMR+ PPV G+F+ A
Sbjct: 320 DTEVCKKVLEEQKQVAKAKEGKGNGKLTWGEVQMMKYTWRVAQELMRMTPPVLGNFKCAW 379
Query: 688 KDTSFGGYDIPKGWQVFWVACGTHLDKDIFDNPTTFDPSRFEHXXXXXXXXXXXPFGAGT 509
+DT+FGG+DIPKGWQVFWVA GTH+DK +F+ P FDPSRFE+ FGAG
Sbjct: 380 RDTTFGGFDIPKGWQVFWVAPGTHMDKKVFEEPEKFDPSRFENPSTSVPPYAYLAFGAGP 439
Query: 508 RMCIGNEFGRIETLVVIHHLVTKFEWSMVDPDEAIKLQPFPHPSLGLPINL 356
R C G +F R+E L++IH+L+TK+ W+ + DE I +P P+P++GLP+ L
Sbjct: 440 RACPGADFSRVEVLLMIHNLITKYHWAEMIIDEPIVREPMPYPAMGLPVKL 490
>emb|CBI25367.3| unnamed protein product [Vitis vinifera]
Length = 496
Score = 354 bits (909), Expect = 5e-95
Identities = 163/351 (46%), Positives = 245/351 (69%), Gaps = 1/351 (0%)
Frame = -2
Query: 1405 RYKLIKAAMVRFLKPECLQHYVKDMDDLIAKKLHSETKDRDTVKAVNLMQIVTFDVACTV 1226
R+KL++ A++ FLKPE +Q V MD ++ ++L E + +D+V+ V LM+ +TF V C++
Sbjct: 140 RHKLVRGAIMNFLKPESIQRSVSRMDSVVQQQLFQELEGKDSVQMVGLMKKITFKVTCSL 199
Query: 1225 LFGIHDETIKNDLLSDFTIAFKAVWSVPINFPGTHFRKGLEARGRIIRRMSPIIMKKKED 1046
LFG+ D +LL DFT A K W+VP + PGT FRK L+ARGRI ++++ ++ ++K
Sbjct: 200 LFGLPDGKETEELLEDFTTALKGAWTVPWDLPGTVFRKALQARGRICKQLAELVRERKAK 259
Query: 1045 LTKGIVYPKTDVICSLLALTEDTEKPITEQEVMDNFFMLMIATYDTSATLLSLMIMKLAK 866
+ +G V D+I SL+ L ++ +P++E+E++DN ++IA++DTS LL L+I LA+
Sbjct: 260 IEEGRVDSHEDIISSLITLRQENGQPLSEEEIIDNLISVVIASHDTSTVLLGLLIRHLAR 319
Query: 865 DRKIYEKIREEQLRILSERQG-ANENLTWNEIQKMKYTWRVAQELMRIIPPVFGSFRKAV 689
D ++ +K+ EEQ ++ ++G N LTW E+Q MKYTWRVAQELMR+ PPV G+F+ A
Sbjct: 320 DTEVCKKVLEEQKQVAKAKEGKGNGKLTWGEVQMMKYTWRVAQELMRMTPPVLGNFKCAW 379
Query: 688 KDTSFGGYDIPKGWQVFWVACGTHLDKDIFDNPTTFDPSRFEHXXXXXXXXXXXPFGAGT 509
+DT+FGG+DIPKGWQVFWVA GTH+DK +F+ P FDPSRFE+ FGAG
Sbjct: 380 RDTTFGGFDIPKGWQVFWVAPGTHMDKKVFEEPEKFDPSRFENPSTSVPPYAYLAFGAGP 439
Query: 508 RMCIGNEFGRIETLVVIHHLVTKFEWSMVDPDEAIKLQPFPHPSLGLPINL 356
R C G +F R+E L++IH+L+TK+ W+ + DE I +P P+P++GLP+ L
Sbjct: 440 RACPGADFSRVEVLLMIHNLITKYHWAEMIIDEPIVREPMPYPAMGLPVKL 490
>emb|CAN70350.1| hypothetical protein VITISV_012582 [Vitis vinifera]
Length = 496
Score = 354 bits (909), Expect = 5e-95
Identities = 163/351 (46%), Positives = 245/351 (69%), Gaps = 1/351 (0%)
Frame = -2
Query: 1405 RYKLIKAAMVRFLKPECLQHYVKDMDDLIAKKLHSETKDRDTVKAVNLMQIVTFDVACTV 1226
R+KL++ A++ FLKPE +Q V MD ++ ++L E + +D+V+ V LM+ +TF V C++
Sbjct: 140 RHKLVRGAIMNFLKPESIQRSVSRMDSVVQQQLFQELEGKDSVQMVGLMKKITFKVTCSL 199
Query: 1225 LFGIHDETIKNDLLSDFTIAFKAVWSVPINFPGTHFRKGLEARGRIIRRMSPIIMKKKED 1046
LFG+ D +LL DFT A K W+VP + PGT FRK L+ARGRI ++++ ++ ++K
Sbjct: 200 LFGLPDGKETEELLEDFTTALKGAWTVPWDLPGTVFRKALQARGRICKQLAQLVRERKAK 259
Query: 1045 LTKGIVYPKTDVICSLLALTEDTEKPITEQEVMDNFFMLMIATYDTSATLLSLMIMKLAK 866
+ +G V D+I SL+ L ++ +P++E+E++DN ++IA++DTS LL L+I LA+
Sbjct: 260 IEEGRVDSHEDIISSLITLRQENGQPLSEEEIIDNLISVVIASHDTSTVLLGLLIRHLAR 319
Query: 865 DRKIYEKIREEQLRILSERQG-ANENLTWNEIQKMKYTWRVAQELMRIIPPVFGSFRKAV 689
D ++ +K+ EEQ ++ ++G N LTW E+Q MKYTWRVAQELMR+ PPV G+F+ A
Sbjct: 320 DTEVCKKVLEEQKQVAKAKEGKGNGKLTWGEVQMMKYTWRVAQELMRMTPPVLGNFKCAW 379
Query: 688 KDTSFGGYDIPKGWQVFWVACGTHLDKDIFDNPTTFDPSRFEHXXXXXXXXXXXPFGAGT 509
+DT+FGG+DIPKGWQVFWVA GTH+DK +F+ P FDPSRFE+ FGAG
Sbjct: 380 RDTTFGGFDIPKGWQVFWVAPGTHMDKKVFEEPEKFDPSRFENPSTSVPPYAYLAFGAGP 439
Query: 508 RMCIGNEFGRIETLVVIHHLVTKFEWSMVDPDEAIKLQPFPHPSLGLPINL 356
R C G +F R+E L++IH+L+TK+ W+ + DE I +P P+P++GLP+ L
Sbjct: 440 RACPGADFSRVEVLLMIHNLITKYHWAEMIIDEPIVREPMPYPAMGLPVKL 490
>ref|XP_003537352.1| PREDICTED: LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like
[Glycine max]
Length = 327
Score = 342 bits (876), Expect = 3e-91
Identities = 166/353 (47%), Positives = 229/353 (64%)
Frame = -2
Query: 1408 CRYKLIKAAMVRFLKPECLQHYVKDMDDLIAKKLHSETKDRDTVKAVNLMQIVTFDVACT 1229
C+Y+L+K M+RFLKPECLQ+YVK
Sbjct: 12 CKYRLVKGEMLRFLKPECLQNYVKK----------------------------------- 36
Query: 1228 VLFGIHDETIKNDLLSDFTIAFKAVWSVPINFPGTHFRKGLEARGRIIRRMSPIIMKKKE 1049
DE + + DFT+AFKA+ S+PIN PGT F +G AR RI+ RM PI+ K++E
Sbjct: 37 ------DEHTREAMFVDFTLAFKAIHSLPINLPGTTFWRGQRARARIVDRMIPIMNKRRE 90
Query: 1048 DLTKGIVYPKTDVICSLLALTEDTEKPITEQEVMDNFFMLMIATYDTSATLLSLMIMKLA 869
+L+KG++ D++ LLAL ++ +P+ + + DN L +A++ TSATL+SLMI KL+
Sbjct: 91 ELSKGVLSSTNDMLSCLLALRDENHQPLDDDLITDNLIFLFVASHGTSATLMSLMIWKLS 150
Query: 868 KDRKIYEKIREEQLRILSERQGANENLTWNEIQKMKYTWRVAQELMRIIPPVFGSFRKAV 689
+D++++ K+ EEQ+ I+ +R+G +TW EIQKMKYTWRVAQELMR+IPP+FGSFRKA+
Sbjct: 151 RDKEVHNKVLEEQMEIIKQREGTXR-VTWAEIQKMKYTWRVAQELMRMIPPLFGSFRKAL 209
Query: 688 KDTSFGGYDIPKGWQVFWVACGTHLDKDIFDNPTTFDPSRFEHXXXXXXXXXXXPFGAGT 509
K+T++ GYDIPKGWQV+W GTH++ DIF+NP FDPS FE+ PFG G
Sbjct: 210 KETNYEGYDIPKGWQVYWATYGTHMNDDIFENPHKFDPSCFENPPKIIPPYSYLPFGTGL 269
Query: 508 RMCIGNEFGRIETLVVIHHLVTKFEWSMVDPDEAIKLQPFPHPSLGLPINLKP 350
+GNEF IETL +IH+ V +EWS V+P+E I QP P+PS+GLPI +KP
Sbjct: 270 HYYVGNEFASIETLTIIHNFVKMYEWSQVNPEEVITRQPMPYPSMGLPIKMKP 322