BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00000737
         (1770 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002272837.1| PREDICTED: bifunctional nitrilase/nitrile hy...   606   e-171
gb|AFK41272.1| unknown [Lotus japonicus]                              603   e-170
ref|XP_002328237.1| nitrilase 1 [Populus trichocarpa] gi|2228377...   603   e-170
sp|Q42966.1|NRL4B_TOBAC RecName: Full=Bifunctional nitrilase/nit...   602   e-170
ref|XP_003538102.1| PREDICTED: bifunctional nitrilase/nitrile hy...   602   e-169

>ref|XP_002272837.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4B-like [Vitis
            vinifera]
          Length = 347

 Score =  606 bits (1563), Expect = e-171
 Identities = 291/353 (82%), Positives = 324/353 (91%)
 Frame = -3

Query: 1654 MALVPTPLIDNNGAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLDKAERLISD 1475
            MAL+P  + D      P+ AEVDMG+DS+A TVRATVVQAST+FYDTPATLDKAERL+++
Sbjct: 1    MALIPATVNDR-----PLFAEVDMGADSSAPTVRATVVQASTVFYDTPATLDKAERLLAE 55

Query: 1474 AALYGSQLVVFPEAFIGGYPRGSNFGLTIGSRTLKGKEDFRKYHASAIDVPGPEVERLGA 1295
            A+ YGSQLVVFPEAFIGGYPRGSNFG+TIG+RT KG+EDFRKYHA+AIDVPGPEV+RL A
Sbjct: 56   ASSYGSQLVVFPEAFIGGYPRGSNFGVTIGNRTAKGREDFRKYHAAAIDVPGPEVDRLAA 115

Query: 1294 MAGKYKVYLVMGVIERERYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGDGSTIP 1115
            MAGKYKVYLVMGVIER+ YTLYCT+LFFDSQGHYLGKHRK+MPT+LER+IWGFGDGSTIP
Sbjct: 116  MAGKYKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKVMPTALERIIWGFGDGSTIP 175

Query: 1114 VFETPIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 935
            V+ETPIGKIG  ICWEN+MPLLRTAMY KGIEIYCAPTADARD+WQ+SMTHIALEGGCFV
Sbjct: 176  VYETPIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADARDIWQSSMTHIALEGGCFV 235

Query: 934  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTILAGPNYEGEALIS 755
            LSANQFCRRKDYPPPPEY FSG D DL+P+SVVCAGGSVIISPSGT+LAGPNY+GEALIS
Sbjct: 236  LSANQFCRRKDYPPPPEYEFSGAD-DLTPDSVVCAGGSVIISPSGTVLAGPNYDGEALIS 294

Query: 754  ADLDFGEIVRAKFDFDVVGHYSRPEVLNLIVRDHPTIPVAFTSAAAKTEGSHK 596
            ADLD GEI RAKFDFDVVGHYSRPEVL+L+V+D+PT PV FTSA+ KTE   K
Sbjct: 295  ADLDLGEIARAKFDFDVVGHYSRPEVLSLVVKDNPTKPVTFTSASVKTEDFQK 347


>gb|AFK41272.1| unknown [Lotus japonicus]
          Length = 346

 Score =  603 bits (1554), Expect = e-170
 Identities = 291/350 (83%), Positives = 320/350 (91%), Gaps = 2/350 (0%)
 Frame = -3

Query: 1654 MALVPT--PLIDNNGAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLDKAERLI 1481
            MALVPT  P I+N     P+IAEVDMGSDS A TVRATVVQASTIFYDTPATLDKAERL+
Sbjct: 1    MALVPTTTPTINNG----PLIAEVDMGSDSNAPTVRATVVQASTIFYDTPATLDKAERLL 56

Query: 1480 SDAALYGSQLVVFPEAFIGGYPRGSNFGLTIGSRTLKGKEDFRKYHASAIDVPGPEVERL 1301
            ++AA YGSQLVVFPEAFIGGYPRGSNFG+ IG RT KG+EDFRKYH+SAIDVPGPEV+RL
Sbjct: 57   AEAAGYGSQLVVFPEAFIGGYPRGSNFGIIIGIRTAKGREDFRKYHSSAIDVPGPEVDRL 116

Query: 1300 GAMAGKYKVYLVMGVIERERYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGDGST 1121
             AMAGKYKV+LVMGVIER+ YTLYCT+LFFDSQGHYLGKHRKLMPT +ERV+WGFGDGST
Sbjct: 117  AAMAGKYKVHLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKLMPTGMERVMWGFGDGST 176

Query: 1120 IPVFETPIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGC 941
            IPVFETP+GKIG +ICWEN+MPLLRTAMY KG+EIYCAPTADAR++WQASMTHIALEGGC
Sbjct: 177  IPVFETPLGKIGAVICWENRMPLLRTAMYAKGVEIYCAPTADAREVWQASMTHIALEGGC 236

Query: 940  FVLSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTILAGPNYEGEAL 761
            FVLSANQFCRRKDYPPPPEY F+GT+EDL+P+SVVCAGGSVIISP G +LAGPNYEGEAL
Sbjct: 237  FVLSANQFCRRKDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPLGNVLAGPNYEGEAL 296

Query: 760  ISADLDFGEIVRAKFDFDVVGHYSRPEVLNLIVRDHPTIPVAFTSAAAKT 611
            ISADLD GEI RAKFDFDVVGHYSRPEVL+L V+DHPT PV F S + K+
Sbjct: 297  ISADLDLGEIARAKFDFDVVGHYSRPEVLSLSVKDHPTNPVTFASTSTKS 346


>ref|XP_002328237.1| nitrilase 1 [Populus trichocarpa] gi|222837752|gb|EEE76117.1|
            nitrilase 1 [Populus trichocarpa]
          Length = 348

 Score =  603 bits (1554), Expect = e-170
 Identities = 290/353 (82%), Positives = 318/353 (90%)
 Frame = -3

Query: 1654 MALVPTPLIDNNGAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLDKAERLISD 1475
            MALVP P  +      P+ AEVDM SDS+A TVRATV+QAST+FYDTPATLDKAER +++
Sbjct: 1    MALVPAPPSET-----PLFAEVDMCSDSSATTVRATVIQASTVFYDTPATLDKAERFLAE 55

Query: 1474 AALYGSQLVVFPEAFIGGYPRGSNFGLTIGSRTLKGKEDFRKYHASAIDVPGPEVERLGA 1295
            AA YGSQLVVFPEAFIGGYPRGS+FG TIGSRT KG+EDFRKYHASAIDVPGPEV+RL A
Sbjct: 56   AAGYGSQLVVFPEAFIGGYPRGSSFGATIGSRTAKGREDFRKYHASAIDVPGPEVDRLAA 115

Query: 1294 MAGKYKVYLVMGVIERERYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGDGSTIP 1115
            MAGKYKVYLVMGVIERE YTLYCT+LFFDSQGHY+GKHRK+MPT++ER++WGFGDGSTIP
Sbjct: 116  MAGKYKVYLVMGVIEREGYTLYCTVLFFDSQGHYMGKHRKVMPTAVERIVWGFGDGSTIP 175

Query: 1114 VFETPIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 935
            VF TPIGKIG  ICWEN+MPLLRTAMYGKGIEIYCAPTAD+RD WQA+MTHIALEGGCFV
Sbjct: 176  VFGTPIGKIGAAICWENRMPLLRTAMYGKGIEIYCAPTADSRDTWQATMTHIALEGGCFV 235

Query: 934  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTILAGPNYEGEALIS 755
            LSANQFCRRKDYPPPPEY F G +EDL+P+SVVCAGGSVIISP GT+LAGPNY+GEALIS
Sbjct: 236  LSANQFCRRKDYPPPPEYVFLGVEEDLTPDSVVCAGGSVIISPLGTVLAGPNYDGEALIS 295

Query: 754  ADLDFGEIVRAKFDFDVVGHYSRPEVLNLIVRDHPTIPVAFTSAAAKTEGSHK 596
            ADLD GEI RAKFDFDVVGHYSRPEVL+L VRDHPT  V FTS +AKTE S K
Sbjct: 296  ADLDLGEIARAKFDFDVVGHYSRPEVLSLTVRDHPTNAVMFTSESAKTEASRK 348


>sp|Q42966.1|NRL4B_TOBAC RecName: Full=Bifunctional nitrilase/nitrile hydratase NIT4B;
            Short=TNIT4B; AltName: Full=Cyanoalanine nitrilase B;
            AltName: Full=Nitrilase 4B gi|1181615|dbj|BAA11770.1|
            nitrilase [Nicotiana tabacum]
          Length = 348

 Score =  602 bits (1553), Expect = e-170
 Identities = 288/353 (81%), Positives = 323/353 (91%)
 Frame = -3

Query: 1654 MALVPTPLIDNNGAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLDKAERLISD 1475
            MALVPTP+++      P+ AEVDMG +S+  TVRATVVQASTIFYDTPATLDKAERL+++
Sbjct: 1    MALVPTPVVNEG----PMFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAE 56

Query: 1474 AALYGSQLVVFPEAFIGGYPRGSNFGLTIGSRTLKGKEDFRKYHASAIDVPGPEVERLGA 1295
            AA YG+QLVVFPEAFIGGYPRGS FG++IG+RT KGKE+FRKYHASAIDVPGPEV+RL A
Sbjct: 57   AASYGAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAA 116

Query: 1294 MAGKYKVYLVMGVIERERYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGDGSTIP 1115
            MAGKYKVYLVMGVIER+ YTLYCT+LFFDSQGHYLGKHRK+MPT+LER+IWGFGDGSTIP
Sbjct: 117  MAGKYKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIP 176

Query: 1114 VFETPIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 935
            V++TP+GKIG  ICWEN+MPLLRTAMY KGIEIYCAPTAD+RD+WQASMTHIALEGGCFV
Sbjct: 177  VYDTPLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFV 236

Query: 934  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTILAGPNYEGEALIS 755
            LSANQFCRRKDYPPPPEY FSGT EDL+P+S+VCAGGSVIISPSG +LAGPNYEGEALIS
Sbjct: 237  LSANQFCRRKDYPPPPEYVFSGT-EDLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALIS 295

Query: 754  ADLDFGEIVRAKFDFDVVGHYSRPEVLNLIVRDHPTIPVAFTSAAAKTEGSHK 596
            ADLD GEI RAKFDFDVVGHY+RPEVL+LIVRDH   PV+FTS ++K E S K
Sbjct: 296  ADLDLGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 348


>ref|XP_003538102.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A-like
            [Glycine max]
          Length = 350

 Score =  602 bits (1552), Expect = e-169
 Identities = 289/353 (81%), Positives = 320/353 (90%)
 Frame = -3

Query: 1654 MALVPTPLIDNNGAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLDKAERLISD 1475
            MALV T    N G   P+ AEVDMGSD  A TVRATVVQASTIFYDTPATLDKAERL+++
Sbjct: 1    MALVTTNPTINEG---PLFAEVDMGSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAE 57

Query: 1474 AALYGSQLVVFPEAFIGGYPRGSNFGLTIGSRTLKGKEDFRKYHASAIDVPGPEVERLGA 1295
            AA YGSQLVVFPEAF+GGYPRGS FGL+IG+RT KG+E+FRKYH++AIDVPGPEV+RL A
Sbjct: 58   AASYGSQLVVFPEAFVGGYPRGSAFGLSIGNRTAKGREEFRKYHSAAIDVPGPEVDRLAA 117

Query: 1294 MAGKYKVYLVMGVIERERYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGDGSTIP 1115
            MAGKYKV+LVMGVIER+ YTLYCT+LFFDSQGHYLGKHRK+MPT+LERVIWGFGDGSTIP
Sbjct: 118  MAGKYKVHLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERVIWGFGDGSTIP 177

Query: 1114 VFETPIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 935
            VFETP+GKIG  ICWEN+MPLLRTAMY KG+EIYCAPTAD+RD+WQASMTHIALEGGCFV
Sbjct: 178  VFETPVGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADSRDVWQASMTHIALEGGCFV 237

Query: 934  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTILAGPNYEGEALIS 755
            LSANQFCRRKDYPPPPEY F+GT+EDL+P+SVVCAGGSVIISPSG +LAGPNY+GEALIS
Sbjct: 238  LSANQFCRRKDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPSGAVLAGPNYDGEALIS 297

Query: 754  ADLDFGEIVRAKFDFDVVGHYSRPEVLNLIVRDHPTIPVAFTSAAAKTEGSHK 596
            ADLD GEI RAKFDFDVVGHYSRPEVL+LIV+DHPT PV FTS + K E   K
Sbjct: 298  ADLDLGEIARAKFDFDVVGHYSRPEVLSLIVKDHPTNPVTFTSTSTKIEDKTK 350