BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00000496
         (1595 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P48496.1|TPIC_SPIOL RecName: Full=Triosephosphate isomerase, ...   408   e-133
ref|XP_002274871.1| PREDICTED: triosephosphate isomerase, chloro...   427   e-132
gb|ADG27841.1| triosephosphate isomerase [Gossypium hirsutum]         414   e-129
ref|XP_004147017.1| PREDICTED: triosephosphate isomerase, chloro...   415   e-127
ref|XP_002529248.1| triosephosphate isomerase, putative [Ricinus...   412   e-127

>sp|P48496.1|TPIC_SPIOL RecName: Full=Triosephosphate isomerase, chloroplastic; Short=TIM;
            Short=Triose-phosphate isomerase; Flags: Precursor
            gi|806312|gb|AAA66289.1| triosephosphate isomerase,
            chloroplast isozyme [Spinacia oleracea]
          Length = 322

 Score =  408 bits (1049), Expect(2) = e-133
 Identities = 205/269 (76%), Positives = 227/269 (84%)
 Frame = +1

Query: 547  NWKCNGTKDSISKLVSDLNNATLEXXXXXXXXXXFVYLDQVKNTLTDRVEISAQNSWVGK 726
            NWKCNGTK+SI+KLVSDLN+ATLE          FVY+DQVK++LT RVEISAQN W+GK
Sbjct: 78   NWKCNGTKESITKLVSDLNSATLEADVDVVVAPPFVYIDQVKSSLTGRVEISAQNCWIGK 137

Query: 727  GGAFTGXXXXXXXXXXXXXXXXXXXXXXXXEISVEQLKDIGCKWVILGHSERRHVIGEDD 906
            GGAFTG                        EISVEQLKD+GC+WVILGHSERRHVIGE +
Sbjct: 138  GGAFTG------------------------EISVEQLKDLGCQWVILGHSERRHVIGEQN 173

Query: 907  QFIGKKAAYALSENLKVIACIGEKLEEREAGKTFDVCFQQLKAFADSVPSWDDVVVAYEP 1086
            +FIGKKAAYAL++ + VIACIGE LEEREAGKTFDVC+QQLKAFAD++PSWD+VVVAYEP
Sbjct: 174  EFIGKKAAYALNQGVGVIACIGELLEEREAGKTFDVCYQQLKAFADALPSWDNVVVAYEP 233

Query: 1087 VWAIGTGKVASPEQAQEVHVAVRDWLKKNVSAEVASKTRIIYGGSVNGSNCAELAKKEDI 1266
            VWAIGTGKVASP+QAQEVHVAVRDWLKKNVS EVASKTRIIYGGSVNG NCAELAK+EDI
Sbjct: 234  VWAIGTGKVASPDQAQEVHVAVRDWLKKNVSEEVASKTRIIYGGSVNGGNCAELAKQEDI 293

Query: 1267 DGFLVGGASLKGPEFATIINSVTSKKVAA 1353
            DGFLVGGASLKGPEFATI+NSVT+KKVAA
Sbjct: 294  DGFLVGGASLKGPEFATIVNSVTAKKVAA 322



 Score = 94.4 bits (233), Expect(2) = e-133
 Identities = 53/83 (63%), Positives = 64/83 (77%), Gaps = 2/83 (2%)
 Frame = +2

Query: 239 MALVSTSLASNPKSTAAAVISSSHFSGLRRSCLKFDQSES-RNSFFNQIDSHLRISSTKK 415
           MA+VSTSLAS   +  +AV  S+ FSGLRRS LK + S S ++SFF  +DSHLR+SS+ +
Sbjct: 1   MAVVSTSLASQITNPNSAV--STQFSGLRRSFLKLENSVSTQSSFFQNVDSHLRLSSSSR 58

Query: 416 GC-RGVVAMAGSGTFFVGGNWKC 481
            C RGVVAMAGSG FFVGGNWKC
Sbjct: 59  RCPRGVVAMAGSGKFFVGGNWKC 81


>ref|XP_002274871.1| PREDICTED: triosephosphate isomerase, chloroplastic-like isoform 1
            [Vitis vinifera]
          Length = 324

 Score =  427 bits (1097), Expect(2) = e-132
 Identities = 216/269 (80%), Positives = 229/269 (85%)
 Frame = +1

Query: 547  NWKCNGTKDSISKLVSDLNNATLEXXXXXXXXXXFVYLDQVKNTLTDRVEISAQNSWVGK 726
            NWKCNGTKDSISKLVSDLN+A LE          FVYLDQVKN+LTDR+EISAQNSWVGK
Sbjct: 80   NWKCNGTKDSISKLVSDLNSAKLEADVDVVVAPPFVYLDQVKNSLTDRIEISAQNSWVGK 139

Query: 727  GGAFTGXXXXXXXXXXXXXXXXXXXXXXXXEISVEQLKDIGCKWVILGHSERRHVIGEDD 906
            GGAFTG                        EISVEQLKDIGC WVILGHSERRHVIGEDD
Sbjct: 140  GGAFTG------------------------EISVEQLKDIGCNWVILGHSERRHVIGEDD 175

Query: 907  QFIGKKAAYALSENLKVIACIGEKLEEREAGKTFDVCFQQLKAFADSVPSWDDVVVAYEP 1086
            QFIGKKAAYALS+ LKVIACIGE L+EREAGKTFDVCFQQ+KAFAD+VPSWDDVV+AYEP
Sbjct: 176  QFIGKKAAYALSQGLKVIACIGELLQEREAGKTFDVCFQQMKAFADAVPSWDDVVIAYEP 235

Query: 1087 VWAIGTGKVASPEQAQEVHVAVRDWLKKNVSAEVASKTRIIYGGSVNGSNCAELAKKEDI 1266
            VWAIGTGKVA+P QAQEVH+AVRDWLKKNVSAEVASKTRIIYGGSVNGSNC+ELAK+EDI
Sbjct: 236  VWAIGTGKVATPLQAQEVHIAVRDWLKKNVSAEVASKTRIIYGGSVNGSNCSELAKQEDI 295

Query: 1267 DGFLVGGASLKGPEFATIINSVTSKKVAA 1353
            DGFLVGGASLKGPEFA I+NSVTSKKVAA
Sbjct: 296  DGFLVGGASLKGPEFAVIVNSVTSKKVAA 324



 Score = 74.7 bits (182), Expect(2) = e-132
 Identities = 49/86 (56%), Positives = 57/86 (66%), Gaps = 6/86 (6%)
 Frame = +2

Query: 242 ALVSTSLASN---PKSTAAAVISSS--HFSGLRRSCLKFDQSESRNSFFNQIDSHLRISS 406
           A+ STSLAS    PK+T  +   SS   FSGLRRS    D S     FF+   S+LRISS
Sbjct: 3   AVTSTSLASQLSTPKATTPSYSYSSATQFSGLRRSFSNLDHSPQ--PFFS---SNLRISS 57

Query: 407 T-KKGCRGVVAMAGSGTFFVGGNWKC 481
           + +K CRG+VAMAGSG FFVGGNWKC
Sbjct: 58  SPRKPCRGIVAMAGSGKFFVGGNWKC 83


>gb|ADG27841.1| triosephosphate isomerase [Gossypium hirsutum]
          Length = 307

 Score =  414 bits (1065), Expect(2) = e-129
 Identities = 209/269 (77%), Positives = 225/269 (83%)
 Frame = +1

Query: 547  NWKCNGTKDSISKLVSDLNNATLEXXXXXXXXXXFVYLDQVKNTLTDRVEISAQNSWVGK 726
            NWKCNGTKDSI+KLVSDLN+A LE          FVYLDQV  +LT R+E+SAQNSW+GK
Sbjct: 63   NWKCNGTKDSITKLVSDLNSAKLETDVDVVVSPPFVYLDQVTASLTSRIEVSAQNSWIGK 122

Query: 727  GGAFTGXXXXXXXXXXXXXXXXXXXXXXXXEISVEQLKDIGCKWVILGHSERRHVIGEDD 906
            GGAFTG                        EISVEQLKDIGCKWVILGHSERRH+IGEDD
Sbjct: 123  GGAFTG------------------------EISVEQLKDIGCKWVILGHSERRHIIGEDD 158

Query: 907  QFIGKKAAYALSENLKVIACIGEKLEEREAGKTFDVCFQQLKAFADSVPSWDDVVVAYEP 1086
            QFIGKKAAYAL+E L VIACIGE LEEREAGKTFDVCF+QLKAFAD VPSWD++V+AYEP
Sbjct: 159  QFIGKKAAYALNEGLGVIACIGELLEEREAGKTFDVCFRQLKAFADVVPSWDNIVIAYEP 218

Query: 1087 VWAIGTGKVASPEQAQEVHVAVRDWLKKNVSAEVASKTRIIYGGSVNGSNCAELAKKEDI 1266
            VWAIGTGKVA+P+QAQEVHVAV DWLKKNVS EVASKTRIIYGGSVNGSNCAELAK+EDI
Sbjct: 219  VWAIGTGKVATPQQAQEVHVAVHDWLKKNVSEEVASKTRIIYGGSVNGSNCAELAKQEDI 278

Query: 1267 DGFLVGGASLKGPEFATIINSVTSKKVAA 1353
            DGFLVGGASLKGPEFATI+NSVTSKKVAA
Sbjct: 279  DGFLVGGASLKGPEFATIVNSVTSKKVAA 307



 Score = 76.3 bits (186), Expect(2) = e-129
 Identities = 36/58 (62%), Positives = 44/58 (75%)
 Frame = +2

Query: 308 HFSGLRRSCLKFDQSESRNSFFNQIDSHLRISSTKKGCRGVVAMAGSGTFFVGGNWKC 481
           HF+GL RS  K D S+S+ SF   I+S LR++S++K CR V AMAGSG FFVGGNWKC
Sbjct: 10  HFNGLHRSSPKLDSSQSQ-SFLQHINSQLRLASSRKPCRAVTAMAGSGKFFVGGNWKC 66


>ref|XP_004147017.1| PREDICTED: triosephosphate isomerase, chloroplastic-like [Cucumis
            sativus] gi|449489711|ref|XP_004158393.1| PREDICTED:
            triosephosphate isomerase, chloroplastic-like [Cucumis
            sativus]
          Length = 306

 Score =  415 bits (1067), Expect(2) = e-127
 Identities = 208/268 (77%), Positives = 225/268 (83%)
 Frame = +1

Query: 547  NWKCNGTKDSISKLVSDLNNATLEXXXXXXXXXXFVYLDQVKNTLTDRVEISAQNSWVGK 726
            NWKCNGTK+SI+KLV+DLNNA LE          FVY++QVK++LT R+EISAQNSWV K
Sbjct: 62   NWKCNGTKESIAKLVADLNNAKLEDDVDVVVAPPFVYIEQVKSSLTSRIEISAQNSWVSK 121

Query: 727  GGAFTGXXXXXXXXXXXXXXXXXXXXXXXXEISVEQLKDIGCKWVILGHSERRHVIGEDD 906
            GGAFTG                        EISVEQLKDIGCKWVILGHSERRHVIGEDD
Sbjct: 122  GGAFTG------------------------EISVEQLKDIGCKWVILGHSERRHVIGEDD 157

Query: 907  QFIGKKAAYALSENLKVIACIGEKLEEREAGKTFDVCFQQLKAFADSVPSWDDVVVAYEP 1086
            QFIGKKAAYALSE L VIACIGE LEEREAGKTFDVCFQQLKA+AD+VPSWD +V+AYEP
Sbjct: 158  QFIGKKAAYALSEGLGVIACIGELLEEREAGKTFDVCFQQLKAYADAVPSWDSIVIAYEP 217

Query: 1087 VWAIGTGKVASPEQAQEVHVAVRDWLKKNVSAEVASKTRIIYGGSVNGSNCAELAKKEDI 1266
            VWAIGTGKVA+PEQAQEVH A+RDWLKKNVS+EVASKTRIIYGGSVNGSNCAELAKKEDI
Sbjct: 218  VWAIGTGKVATPEQAQEVHAAIRDWLKKNVSSEVASKTRIIYGGSVNGSNCAELAKKEDI 277

Query: 1267 DGFLVGGASLKGPEFATIINSVTSKKVA 1350
            DGFLVGGASLKGPEF TI+NSVT+KKVA
Sbjct: 278  DGFLVGGASLKGPEFGTIVNSVTAKKVA 305



 Score = 68.6 bits (166), Expect(2) = e-127
 Identities = 41/81 (50%), Positives = 49/81 (60%)
 Frame = +2

Query: 239 MALVSTSLASNPKSTAAAVISSSHFSGLRRSCLKFDQSESRNSFFNQIDSHLRISSTKKG 418
           MA VSTSLAS              FS LR S    D S   +S F+ + S +R++S++KG
Sbjct: 1   MAAVSTSLASR-------------FSPLRFSSSNSDIS---HSLFHNVHSQIRLASSRKG 44

Query: 419 CRGVVAMAGSGTFFVGGNWKC 481
            RGVV MAGSG FFVGGNWKC
Sbjct: 45  SRGVVTMAGSGKFFVGGNWKC 65


>ref|XP_002529248.1| triosephosphate isomerase, putative [Ricinus communis]
            gi|223531284|gb|EEF33126.1| triosephosphate isomerase,
            putative [Ricinus communis]
          Length = 313

 Score =  412 bits (1060), Expect(2) = e-127
 Identities = 207/269 (76%), Positives = 226/269 (84%)
 Frame = +1

Query: 547  NWKCNGTKDSISKLVSDLNNATLEXXXXXXXXXXFVYLDQVKNTLTDRVEISAQNSWVGK 726
            NWKCNGTK+SISKLVSDLN+A LE          F+Y+DQVK++LTDR+EISAQNSWV K
Sbjct: 69   NWKCNGTKESISKLVSDLNDAKLEADVDVVVAPPFIYIDQVKSSLTDRIEISAQNSWVSK 128

Query: 727  GGAFTGXXXXXXXXXXXXXXXXXXXXXXXXEISVEQLKDIGCKWVILGHSERRHVIGEDD 906
            GGAFTG                        EISVEQL DIGCKWVILGHSERRHVIGE+D
Sbjct: 129  GGAFTG------------------------EISVEQLNDIGCKWVILGHSERRHVIGEND 164

Query: 907  QFIGKKAAYALSENLKVIACIGEKLEEREAGKTFDVCFQQLKAFADSVPSWDDVVVAYEP 1086
            +FIGKKAAYALS+ L VIACIGE LEEREAGKTFDVCFQQLKA+AD+VPSWD +V+AYEP
Sbjct: 165  EFIGKKAAYALSQGLGVIACIGELLEEREAGKTFDVCFQQLKAYADAVPSWDKIVIAYEP 224

Query: 1087 VWAIGTGKVASPEQAQEVHVAVRDWLKKNVSAEVASKTRIIYGGSVNGSNCAELAKKEDI 1266
            VWAIGTGKVA+P+QAQEVHVA+RDWLKKN+S EVASKTRIIYGGSVNG NCAELAK+EDI
Sbjct: 225  VWAIGTGKVATPQQAQEVHVALRDWLKKNISEEVASKTRIIYGGSVNGGNCAELAKQEDI 284

Query: 1267 DGFLVGGASLKGPEFATIINSVTSKKVAA 1353
            DGFLVGGASLKGPEFATIINSVTSKKVAA
Sbjct: 285  DGFLVGGASLKGPEFATIINSVTSKKVAA 313



 Score = 70.9 bits (172), Expect(2) = e-127
 Identities = 43/83 (51%), Positives = 54/83 (65%), Gaps = 2/83 (2%)
 Frame = +2

Query: 239 MALVSTSLASNPKSTAAAVISSSHFSGLRRSCLKFDQSESRN-SFFNQIDSHLR-ISSTK 412
           MA++STSL+             S FSGLR+SC K  ++ S + SF    +S LR ISS++
Sbjct: 1   MAMLSTSLSG-----------PSQFSGLRQSCPKLLETHSLSQSFLRHFNSQLRSISSSR 49

Query: 413 KGCRGVVAMAGSGTFFVGGNWKC 481
           K  R VVAMAG+GTFFVGGNWKC
Sbjct: 50  KPSRHVVAMAGTGTFFVGGNWKC 72