BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00000367
         (694 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   246   3e-63
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   121   1e-25
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   121   1e-25
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              117   2e-24
ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   117   2e-24

>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  246 bits (628), Expect = 3e-63
 Identities = 138/199 (69%), Positives = 138/199 (69%)
 Frame = +1

Query: 97  MDLQIAXXXXXXXXXXXXXXXXXXXXXXXXXXHPWPLIGNLPILFTNTEVPLHITLANMA 276
           MDLQIA                          HPWPLIGNLPILFTNTEVPLHITLANMA
Sbjct: 1   MDLQIALFSLIPVILVFILLLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMA 60

Query: 277 RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRXXXXXXXXXXXXXXXXXX 456
           RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIR                  
Sbjct: 61  RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIR----------MSFRLKHH 110

Query: 457 XXXXXXXXXXXXXXXXXRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKIS 636
                            RKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKIS
Sbjct: 111 IKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKIS 170

Query: 637 QFVFGTLLNILGNVVFSKD 693
           QFVFGTLLNILGNVVFSKD
Sbjct: 171 QFVFGTLLNILGNVVFSKD 189


>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
           AltName: Full=Cytochrome P450 80B1
           gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
           3'-hydroxylase [Eschscholzia californica]
          Length = 487

 Score =  121 bits (304), Expect = 1e-25
 Identities = 67/166 (40%), Positives = 99/166 (59%)
 Frame = +1

Query: 196 PWPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTHD 375
           PWP++GNL  L    E P H   A +A+T+G I  L +GT+  V+AST+ AA EILKTHD
Sbjct: 34  PWPIVGNLLQL---GEKP-HAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHD 89

Query: 376 RIFSARHIRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKIVRTEIFSPKMLQA 555
           RI SAR++                                    RK+ RTE+F+ KM+++
Sbjct: 90  RILSARYV----------FQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIES 139

Query: 556 QSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKD 693
           Q+HVRE+K  E++++L  K+G+ VKI + +FGTL+NI GN++FS++
Sbjct: 140 QAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQN 185


>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
           AltName: Full=Cytochrome P450 80B2
           gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
           3'-hydroxylase [Eschscholzia californica]
          Length = 488

 Score =  121 bits (304), Expect = 1e-25
 Identities = 67/166 (40%), Positives = 99/166 (59%)
 Frame = +1

Query: 196 PWPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTHD 375
           PWP++GNL  L    E P H   A +A+T+G I  L +GT+  V+AST+ AA EILKTHD
Sbjct: 35  PWPIVGNLLQL---GEKP-HAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHD 90

Query: 376 RIFSARHIRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKIVRTEIFSPKMLQA 555
           RI SAR++                                    RK+ RTE+F+ KM+++
Sbjct: 91  RILSARYV----------FQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIES 140

Query: 556 QSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKD 693
           Q+HVRE+K  E++++L  K+G+ VKI + +FGTL+NI GN++FS++
Sbjct: 141 QAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQN 186


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  117 bits (294), Expect = 2e-24
 Identities = 69/167 (41%), Positives = 92/167 (55%)
 Frame = +1

Query: 193  HPWPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTH 372
            +PWP+IGNL  +  N     H  LAN+A+ HGP+M L LGTQ  V+AS+  AAME+LKTH
Sbjct: 583  YPWPIIGNLLQMGKNP----HAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTH 638

Query: 373  DRIFSARHIRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKIVRTEIFSPKMLQ 552
            DR  S R++                                    R I R E+FS K ++
Sbjct: 639  DRALSGRYL-------SSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAME 691

Query: 553  AQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKD 693
            +Q  +RE+KV EL++FL +KEG+VVK+   VF T+ NIL N  FS D
Sbjct: 692  SQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMD 738



 Score =  117 bits (294), Expect = 2e-24
 Identities = 68/167 (40%), Positives = 93/167 (55%)
 Frame = +1

Query: 193  HPWPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTH 372
            +PWP+IGNL  L  N     H+ LA++A+ HGP+M L LGTQ  V+AS+  AA+E+LKTH
Sbjct: 1088 YPWPIIGNLLQLGKNP----HVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTH 1143

Query: 373  DRIFSARHIRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKIVRTEIFSPKMLQ 552
            DR  S R++                                    R I RTE+FS K ++
Sbjct: 1144 DRTLSGRYV-------SSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAME 1196

Query: 553  AQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKD 693
            +   +RE+KV EL++FL +KEG+VVK+   VF T+ NIL N  FS D
Sbjct: 1197 SHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMD 1243



 Score =  116 bits (291), Expect = 4e-24
 Identities = 67/167 (40%), Positives = 94/167 (56%)
 Frame = +1

Query: 193 HPWPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTH 372
           +PWP+IGNL  +  N    LH+ LAN+A+ HGP+M L LGTQ  V+AS++ AAME+LKTH
Sbjct: 43  YPWPIIGNLLQMGGN----LHVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTH 98

Query: 373 DRIFSARHIRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKIVRTEIFSPKMLQ 552
           DR  S R++                                    + I R E+FS K ++
Sbjct: 99  DRTLSGRYV-------STTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAME 151

Query: 553 AQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKD 693
           ++  +RE+KV EL++FL  KEG+VVK+   V+ T+ NIL N  FS D
Sbjct: 152 SRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILSNKFFSID 198


>ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
           [Vitis vinifera]
          Length = 515

 Score =  117 bits (294), Expect = 2e-24
 Identities = 68/167 (40%), Positives = 93/167 (55%)
 Frame = +1

Query: 193 HPWPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTH 372
           +PWP+IGNL  L  N     H+ LA++A+ HGP+M L LGTQ  V+AS+  AA+E+LKTH
Sbjct: 43  YPWPIIGNLLQLGKNP----HVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTH 98

Query: 373 DRIFSARHIRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKIVRTEIFSPKMLQ 552
           DR  S R++                                    R I RTE+FS K ++
Sbjct: 99  DRTLSGRYV-------SSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAME 151

Query: 553 AQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKD 693
           +   +RE+KV EL++FL +KEG+VVK+   VF T+ NIL N  FS D
Sbjct: 152 SHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMD 198