BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00000180
         (1745 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002324349.1| predicted protein [Populus trichocarpa] gi|2...   353   e-127
ref|XP_002515388.1| Aspartic proteinase nepenthesin-2 precursor,...   353   e-122
ref|XP_002285593.1| PREDICTED: aspartic proteinase nepenthesin-2...   341   e-120
ref|XP_002283470.1| PREDICTED: aspartic proteinase nepenthesin-2...   329   e-119
ref|XP_003532146.1| PREDICTED: aspartic proteinase nepenthesin-1...   330   e-119

>ref|XP_002324349.1| predicted protein [Populus trichocarpa] gi|222865783|gb|EEF02914.1|
            predicted protein [Populus trichocarpa]
          Length = 490

 Score =  353 bits (907), Expect(2) = e-127
 Identities = 174/268 (64%), Positives = 203/268 (75%), Gaps = 2/268 (0%)
 Frame = +2

Query: 713  CSSSTCVYAIQYGDQSYSIGFFGQETLTLSPTDAFPNFKFGCGQNNDGLYGKSAGLLGLG 892
            C+SS CVY IQYGD S+S+GFFG E LTL+ TDAF N  FGCGQNN GL+G SAGLLGLG
Sbjct: 223  CASSACVYGIQYGDSSFSVGFFGTEKLTLTSTDAFNNIYFGCGQNNQGLFGGSAGLLGLG 282

Query: 893  RDKISLVSQTALKYKKLFSYCLPAHSS--GFLALGAPSSTAAKFTPLLADSRGPSFYFLT 1066
            RDK+S+VSQTA KY K+FSYCLP+ SS  GFL  G  +S  AKFTPL   S GPSFY L 
Sbjct: 283  RDKLSVVSQTAQKYNKIFSYCLPSSSSSTGFLTFGGSASKNAKFTPLSTISAGPSFYGLD 342

Query: 1067 LQGISVGGKNLAISPSAFSVGGTIIDSGTVVTRLPPSVYSALRTAFRQAMTTYPSAPALS 1246
              GISVGGK LAIS S FS  G IIDSGTV+TRLPP+ YSALR +FR  M+ YP   ALS
Sbjct: 343  FTGISVGGKKLAISASVFSTAGAIIDSGTVITRLPPAAYSALRASFRNLMSKYPMTKALS 402

Query: 1247 ILDTCYDLSGFKTVSIPKILLHFAGGTDLDVPPTGLLFAQSTSQACLAFAGNSDAGDVAI 1426
            ILDTCYD S + T+S+PKI   F+ G ++D+  TG+L+A S SQ CLAFAGNSDA DV I
Sbjct: 403  ILDTCYDFSSYTTISVPKIGFSFSSGIEVDIDATGILYASSLSQVCLAFAGNSDATDVFI 462

Query: 1427 IGNKQQQTVEIVFDVAGGKLGFGPGGCS 1510
             GN QQ+T+E+ +D + GK+GF PGGCS
Sbjct: 463  FGNVQQKTLEVFYDGSAGKVGFAPGGCS 490



 Score =  129 bits (324), Expect(2) = e-127
 Identities = 74/174 (42%), Positives = 102/174 (58%), Gaps = 9/174 (5%)
 Frame = +1

Query: 121 KAYGTEGTFASNIPHTYHHAIEIQSLLPRTTC---SELPSRHRGKKGAVLPMFNKNGPCS 291
           K Y  EG   +   H+  H+IE+ SLLP  +C   +++ S +  K  A L + +K+GPCS
Sbjct: 31  KGYALEGRKVAESHHS--HSIEVSSLLPSASCKPSTKVLSNNDNK--ASLKVVHKHGPCS 86

Query: 292 PLSNKDNIQKTINHTQILIQDQSRVHSIHSRISK------KGVSITPEEARLPANSGSSL 453
            LS +D       HT+IL+QDQSRV SIHSR+S       K V +T +   +PA  GS++
Sbjct: 87  KLS-QDEASAAPTHTEILLQDQSRVKSIHSRLSNSKTSGGKDVKVT-DSTTIPAKDGSTV 144

Query: 454 XXXXXXXXXXXXXPKKDLPVVFDTGSDLTWIQCQPCVGGCYSQRDPIFDPIKSS 615
                        PKKDL ++FDTGSD+TW QCQPC   CY Q++ IFDP +S+
Sbjct: 145 GSGNYIVTVGLGTPKKDLSLIFDTGSDITWTQCQPCARSCYKQKEQIFDPSQST 198


>ref|XP_002515388.1| Aspartic proteinase nepenthesin-2 precursor, putative [Ricinus
            communis] gi|223545332|gb|EEF46837.1| Aspartic proteinase
            nepenthesin-2 precursor, putative [Ricinus communis]
          Length = 494

 Score =  353 bits (907), Expect(2) = e-122
 Identities = 172/268 (64%), Positives = 208/268 (77%), Gaps = 2/268 (0%)
 Frame = +2

Query: 713  CSSSTCVYAIQYGDQSYSIGFFGQETLTLSPTDAFPNFKFGCGQNNDGLYGKSAGLLGLG 892
            C+SSTCVY IQYGD S+SIGFFG+E L+L+ TD F +F FGCGQNN GL+G +AGLLGLG
Sbjct: 227  CASSTCVYGIQYGDSSFSIGFFGKEKLSLTATDVFNDFYFGCGQNNKGLFGGAAGLLGLG 286

Query: 893  RDKISLVSQTALKYKKLFSYCLPAHSS--GFLALGAPSSTAAKFTPLLADSRGPSFYFLT 1066
            RDK+SLVSQTA +Y K+FSYCLP+ SS  GFL  G  +S +A FTPL   S G SFY L 
Sbjct: 287  RDKLSLVSQTAQRYNKIFSYCLPSSSSSTGFLTFGGSTSKSASFTPLATISGGSSFYGLD 346

Query: 1067 LQGISVGGKNLAISPSAFSVGGTIIDSGTVVTRLPPSVYSALRTAFRQAMTTYPSAPALS 1246
            L GISVGG+ LAISPS FS  GTIIDSGTV+TRLPP+ YSAL + FR+ M+ YP+APALS
Sbjct: 347  LTGISVGGRKLAISPSVFSTAGTIIDSGTVITRLPPAAYSALSSTFRKLMSQYPAAPALS 406

Query: 1247 ILDTCYDLSGFKTVSIPKILLHFAGGTDLDVPPTGLLFAQSTSQACLAFAGNSDAGDVAI 1426
            ILDTC+D S   T+S+PKI L F+GG  +D+  TG+ +    +Q CLAFAGNSDA DVAI
Sbjct: 407  ILDTCFDFSNHDTISVPKIGLFFSGGVVVDIDKTGIFYVNDLTQVCLAFAGNSDASDVAI 466

Query: 1427 IGNKQQQTVEIVFDVAGGKLGFGPGGCS 1510
             GN QQ+T+E+V+D A G++GF P GCS
Sbjct: 467  FGNVQQKTLEVVYDGAAGRVGFAPAGCS 494



 Score =  112 bits (279), Expect(2) = e-122
 Identities = 67/166 (40%), Positives = 89/166 (53%), Gaps = 3/166 (1%)
 Frame = +1

Query: 127 YGTEGTFASNIPHTYHHAIEIQSLLPRTTCSELPSRHRGKKGAVLPMFNKNGPCSPLSNK 306
           Y  EG   +   HT H  I + SLLP  +C         +  A L + +K+GPCS L   
Sbjct: 42  YAFEGRKFAESQHT-HTTIHLTSLLPAASCKPSTQVPSIENKAFLKVVHKHGPCSDLRQG 100

Query: 307 DNIQKTINHTQILIQDQSRVHSIHSRISK-KGVSITPEEAR--LPANSGSSLXXXXXXXX 477
              +       IL+QDQSRV SIHS++SK  G+S     A   LPA  GS +        
Sbjct: 101 HKAEAQY----ILLQDQSRVDSIHSKLSKDSGLSDVKATAATTLPAKDGSIIGSGNYFVT 156

Query: 478 XXXXXPKKDLPVVFDTGSDLTWIQCQPCVGGCYSQRDPIFDPIKSS 615
                PKKD  ++FDTGSDLTW QC+PCV  CY+Q++ IF+P +S+
Sbjct: 157 VGLGTPKKDFSLIFDTGSDLTWTQCEPCVKSCYNQKEAIFNPSQST 202


>ref|XP_002285593.1| PREDICTED: aspartic proteinase nepenthesin-2 [Vitis vinifera]
          Length = 481

 Score =  341 bits (875), Expect(2) = e-120
 Identities = 166/269 (61%), Positives = 206/269 (76%), Gaps = 4/269 (1%)
 Frame = +2

Query: 713  CSSSTCVYAIQYGDQSYSIGFFGQETLTLSPTDAFPNFKFGCGQNNDGLYGKSAGLLGLG 892
            CS+STCVY IQYGDQSYS+GFF Q+ L L+ TD F NF FGCGQNN GL+   AGL+GLG
Sbjct: 212  CSASTCVYGIQYGDQSYSVGFFAQDKLALTSTDVFNNFLFGCGQNNRGLFVGVAGLIGLG 271

Query: 893  RDKISLVSQTALKYKKLFSYCLPAHSS--GFLALGAPSST--AAKFTPLLADSRGPSFYF 1060
            R+ +SLVSQTA KY KLFSYCLP+ SS  G+L  G+   T  A KFTP L +S+GPSFYF
Sbjct: 272  RNALSLVSQTAQKYGKLFSYCLPSTSSSTGYLTFGSGGGTSKAVKFTPSLVNSQGPSFYF 331

Query: 1061 LTLQGISVGGKNLAISPSAFSVGGTIIDSGTVVTRLPPSVYSALRTAFRQAMTTYPSAPA 1240
            L L  ISVGG+ L+ S S FS  GTIIDSGTV++RLPP+ YS LR +F+Q M+ YP A  
Sbjct: 332  LNLIAISVGGRKLSTSASVFSTAGTIIDSGTVISRLPPTAYSDLRASFQQQMSKYPKAAP 391

Query: 1241 LSILDTCYDLSGFKTVSIPKILLHFAGGTDLDVPPTGLLFAQSTSQACLAFAGNSDAGDV 1420
             SILDTCYD S + TV +PKI L+F+ G ++D+ P+G+ +  + SQ CLAFAGNSDA D+
Sbjct: 392  ASILDTCYDFSQYDTVDVPKINLYFSDGAEMDLDPSGIFYILNISQVCLAFAGNSDATDI 451

Query: 1421 AIIGNKQQQTVEIVFDVAGGKLGFGPGGC 1507
            AI+GN QQ+T ++V+DVAGG++GF PGGC
Sbjct: 452  AILGNVQQKTFDVVYDVAGGRIGFAPGGC 480



 Score =  119 bits (299), Expect(2) = e-120
 Identities = 67/159 (42%), Positives = 95/159 (59%), Gaps = 3/159 (1%)
 Frame = +1

Query: 148 ASNIPHTYHHAIEIQSLLPRTTCSELPSRHRGKKGAVLPMFNKNGPCSPLSNKDNIQKTI 327
           A + P T H+ + I SL+P + CS  PS     K A L + +K+GPCS LS      ++ 
Sbjct: 34  ALSTPSTLHN-VHITSLMPSSVCS--PSPKGDDKRASLEVIHKHGPCSKLSQDKG--RSP 88

Query: 328 NHTQILIQDQSRVHSIHSRISKK---GVSITPEEARLPANSGSSLXXXXXXXXXXXXXPK 498
           + TQ+L QD+SRV+SI SR++K    G  +   +  LP+ SGS++             PK
Sbjct: 89  SRTQMLDQDESRVNSIRSRLAKNPADGGKLKGSKVTLPSKSGSTIGTGNYVVTVGLGTPK 148

Query: 499 KDLPVVFDTGSDLTWIQCQPCVGGCYSQRDPIFDPIKSS 615
           +DL  +FDTGSDLTW QC+PC   CY Q++PIF+P KS+
Sbjct: 149 RDLTFIFDTGSDLTWTQCEPCARYCYHQQEPIFNPSKST 187


>ref|XP_002283470.1| PREDICTED: aspartic proteinase nepenthesin-2-like [Vitis vinifera]
          Length = 490

 Score =  329 bits (843), Expect(2) = e-119
 Identities = 160/270 (59%), Positives = 206/270 (76%), Gaps = 4/270 (1%)
 Frame = +2

Query: 713  CSSSTCVYAIQYGDQSYSIGFFGQETLTLSPTDAFPNFKFGCGQNNDGLYGKSAGLLGLG 892
            CSSSTC+Y I+YGD SYSIGFF +E L+L+ TD F NF+FGCGQNN GL+G +AGLLGL 
Sbjct: 221  CSSSTCLYGIRYGDGSYSIGFFAREKLSLTSTDVFNNFQFGCGQNNRGLFGGTAGLLGLA 280

Query: 893  RDKISLVSQTALKYKKLFSYCLPAHSS--GFLALGAPS--STAAKFTPLLADSRGPSFYF 1060
            R+ +SLVSQTA KY K+FSYCLP+ SS  G+L+ G+    S A KFTP   +S  PSFYF
Sbjct: 281  RNPLSLVSQTAQKYGKVFSYCLPSSSSSTGYLSFGSGDGDSKAVKFTPSEVNSDYPSFYF 340

Query: 1061 LTLQGISVGGKNLAISPSAFSVGGTIIDSGTVVTRLPPSVYSALRTAFRQAMTTYPSAPA 1240
            L + GISVG + L I  S FS  GTIIDSGTV++RLPP+VYS+++  FR+ M+ YP    
Sbjct: 341  LDMVGISVGERKLPIPKSVFSTAGTIIDSGTVISRLPPTVYSSVQKVFRELMSDYPRVKG 400

Query: 1241 LSILDTCYDLSGFKTVSIPKILLHFAGGTDLDVPPTGLLFAQSTSQACLAFAGNSDAGDV 1420
            +SILDTCYDLS +KTV +PKI+L+F+GG ++D+ P G+++    SQ CLAFAGNSD  +V
Sbjct: 401  VSILDTCYDLSKYKTVKVPKIILYFSGGAEMDLAPEGIIYVLKVSQVCLAFAGNSDDDEV 460

Query: 1421 AIIGNKQQQTVEIVFDVAGGKLGFGPGGCS 1510
            AIIGN QQ+T+ +V+D A G++GF P GC+
Sbjct: 461  AIIGNVQQKTIHVVYDDAEGRVGFAPSGCN 490



 Score =  127 bits (318), Expect(2) = e-119
 Identities = 71/167 (42%), Positives = 97/167 (58%), Gaps = 5/167 (2%)
 Frame = +1

Query: 127 YGTEGTFASNIPHTY--HHAIEIQSLLPRTTCSELPSRHRGKKGAVLPMFNKNGPCSPLS 300
           +  EG  ++   H    HH + I SL+P + CS  P  H  +  A L + +K+GPCS L 
Sbjct: 33  FAIEGRESAESHHVQPIHHNVHITSLMPSSACSPSPKGHDQR--ASLEVVHKHGPCSKL- 89

Query: 301 NKDNIQKTINHTQILIQDQSRVHSIHSRISKK---GVSITPEEARLPANSGSSLXXXXXX 471
            + +   + +HTQIL QD+SRV SI SR++K    G ++   +A LP+ S S+L      
Sbjct: 90  -RPHKANSPSHTQILAQDESRVASIQSRLAKNLAGGSNLKASKATLPSKSASTLGSGNYV 148

Query: 472 XXXXXXXPKKDLPVVFDTGSDLTWIQCQPCVGGCYSQRDPIFDPIKS 612
                  PK+DL  +FDTGSDLTW QC+PCVG CY QR+ IFDP  S
Sbjct: 149 VTVGLGSPKRDLTFIFDTGSDLTWTQCEPCVGYCYQQREHIFDPSTS 195


>ref|XP_003532146.1| PREDICTED: aspartic proteinase nepenthesin-1-like [Glycine max]
          Length = 488

 Score =  330 bits (846), Expect(2) = e-119
 Identities = 161/269 (59%), Positives = 198/269 (73%), Gaps = 4/269 (1%)
 Frame = +2

Query: 713  CSSST--CVYAIQYGDQSYSIGFFGQETLTLSPTDAFPNFKFGCGQNNDGLYGKSAGLLG 886
            CS+ST  C+Y IQYGD S+S+G+F +E L+++ TD   NF FGCGQNN GL+G SAGL+G
Sbjct: 219  CSASTKACIYGIQYGDSSFSVGYFSRERLSVTATDIVDNFLFGCGQNNQGLFGGSAGLIG 278

Query: 887  LGRDKISLVSQTALKYKKLFSYCLPAHSS--GFLALGAPSSTAAKFTPLLADSRGPSFYF 1060
            LGR  IS V QTA  Y+K+FSYCLPA SS  G L+ G  +++  K+TP    SRG SFY 
Sbjct: 279  LGRHPISFVQQTAAVYRKIFSYCLPATSSSTGRLSFGTTTTSYVKYTPFSTISRGSSFYG 338

Query: 1061 LTLQGISVGGKNLAISPSAFSVGGTIIDSGTVVTRLPPSVYSALRTAFRQAMTTYPSAPA 1240
            L + GISVGG  L +S S FS GG IIDSGTV+TRLPP+ Y+ALR+AFRQ M+ YPSA  
Sbjct: 339  LDITGISVGGAKLPVSSSTFSTGGAIIDSGTVITRLPPTAYTALRSAFRQGMSKYPSAGE 398

Query: 1241 LSILDTCYDLSGFKTVSIPKILLHFAGGTDLDVPPTGLLFAQSTSQACLAFAGNSDAGDV 1420
            LSILDTCYDLSG++  SIPKI   FAGG  + +PP G+L+  S  Q CLAFA N D  DV
Sbjct: 399  LSILDTCYDLSGYEVFSIPKIDFSFAGGVTVQLPPQGILYVASAKQVCLAFAANGDDSDV 458

Query: 1421 AIIGNKQQQTVEIVFDVAGGKLGFGPGGC 1507
             I GN QQ+T+E+V+DV GG++GFG GGC
Sbjct: 459  TIYGNVQQKTIEVVYDVGGGRIGFGAGGC 487



 Score =  125 bits (315), Expect(2) = e-119
 Identities = 71/160 (44%), Positives = 96/160 (60%), Gaps = 5/160 (3%)
 Frame = +1

Query: 151 SNIPHTYHHAIEIQSLLPRTTCSELPSRHRGKKGAVLPMFNKNGPCSPLSNKDNIQKTIN 330
           SN  H Y H + + SLLP ++CS   S    K+ A L + +K+GPCS L+N D   K+  
Sbjct: 37  SNNLHQYTHLVHLSSLLPSSSCSS--SAKGPKRKASLEVVHKHGPCSQLNNHDGKAKSKT 94

Query: 331 -HTQILIQDQSRVHSIHSRISK---KGVSITP-EEARLPANSGSSLXXXXXXXXXXXXXP 495
            H++IL QD+ RV  I+SRISK   +  S++  +   LPA SGS +             P
Sbjct: 95  PHSEILNQDKERVKYINSRISKNLGQDSSVSELDSVTLPAKSGSLIGSGNYFVVVGLGTP 154

Query: 496 KKDLPVVFDTGSDLTWIQCQPCVGGCYSQRDPIFDPIKSS 615
           K+DL ++FDTGSDLTW QC+PC   CY Q+D IFDP KS+
Sbjct: 155 KRDLSLIFDTGSDLTWTQCEPCARSCYKQQDAIFDPSKST 194