BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00000107
         (984 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|A2A1A0.1|NCS1_COPJA RecName: Full=S-norcoclaurine synthase 1;...   157   7e-59
ref|XP_002279625.1| PREDICTED: S-norcoclaurine synthase 1 [Vitis...   155   4e-55
ref|XP_002527641.1| Flavonol synthase/flavanone 3-hydroxylase, p...   160   6e-54
emb|CAN69330.1| hypothetical protein VITISV_016336 [Vitis vinifera]   157   2e-52
ref|XP_002279610.1| PREDICTED: S-norcoclaurine synthase 1-like [...   149   1e-51

>sp|A2A1A0.1|NCS1_COPJA RecName: Full=S-norcoclaurine synthase 1; Short=CjNCS1
           gi|123720767|dbj|BAF45337.1| norcoclaurine synthase
           [Coptis japonica var. dissecta]
           gi|301072256|gb|ADK56103.1| 2OG/Fe(II)-dependent
           dioxygenase-like protein [synthetic construct]
          Length = 352

 Score =  157 bits (396), Expect(2) = 7e-59
 Identities = 87/195 (44%), Positives = 110/195 (56%)
 Frame = -1

Query: 984 IEKMKVDMEDFFRLPLEEKKVYGQLPDSTEGYGQSFVKSDEQKLEWADMHLLITXXXXXX 805
           IEKMKVD EDFFRLP +EK  Y QLP+  EGYGQ+FV S+EQKL+WADMH LIT      
Sbjct: 94  IEKMKVDTEDFFRLPFKEKNAYRQLPNGMEGYGQAFVTSEEQKLDWADMHFLITKPVQER 153

Query: 804 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNNPTSFSESVNKFSKDVQEVAMCLIGLMAR 625
                                          +PTSF E++ K+S ++Q+VAMCL G+MA+
Sbjct: 154 NMRFWP------------------------TSPTSFRETMEKYSMELQKVAMCLTGMMAK 189

Query: 624 NLGLESQVLTKPFENCIQSVRMNYYPPCRYSDKVLGLTPHSDASSXXXXXXXXXXXXXQI 445
           NLGLES++LTKP    + +      P      + LGL+PHSDA+               I
Sbjct: 190 NLGLESEILTKPLRT-VFNREDELLPSMSSCGEGLGLSPHSDATGLTLLIQVNEVNGLHI 248

Query: 444 KKNGKWFPVKPILGA 400
           KK+ KW P+KPILGA
Sbjct: 249 KKDEKWVPIKPILGA 263



 Score = 97.8 bits (242), Expect(2) = 7e-59
 Identities = 48/73 (65%), Positives = 55/73 (75%)
 Frame = -2

Query: 329 YKSIEHRVVINPDKERLSIAVFHDPEYSAIIEPVQDLVKKLGARYKTVRHADYLNQSINN 150
           YKSIEHR VIN DKERLSIA FHDPEY   I P+ DLVK+ G +YKT+ + DYL +S N 
Sbjct: 280 YKSIEHRAVINTDKERLSIAAFHDPEYGTKIGPLPDLVKENGVKYKTIDYEDYLIRSSNI 339

Query: 149 KLDSKTFLDQFKL 111
           KLD K+ LDQ KL
Sbjct: 340 KLDGKSLLDQMKL 352


>ref|XP_002279625.1| PREDICTED: S-norcoclaurine synthase 1 [Vitis vinifera]
           gi|297742182|emb|CBI33969.3| unnamed protein product
           [Vitis vinifera]
          Length = 349

 Score =  155 bits (393), Expect(2) = 4e-55
 Identities = 81/192 (42%), Positives = 109/192 (56%)
 Frame = -1

Query: 984 IEKMKVDMEDFFRLPLEEKKVYGQLPDSTEGYGQSFVKSDEQKLEWADMHLLITXXXXXX 805
           IEKMK+D+++FF+LPLEEK  Y Q P++ EGYGQ+FV S +QKL+W DM  L        
Sbjct: 88  IEKMKMDVQEFFKLPLEEKNAYAQPPNNIEGYGQAFVVSQDQKLDWGDMLFLFPLPASLR 147

Query: 804 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNNPTSFSESVNKFSKDVQEVAMCLIGLMAR 625
                                          NPTSF E+++ +S ++  V++ L+  MA+
Sbjct: 148 NMRLWP------------------------ENPTSFRETLDNYSSELHRVSINLLRSMAK 183

Query: 624 NLGLESQVLTKPFENCIQSVRMNYYPPCRYSDKVLGLTPHSDASSXXXXXXXXXXXXXQI 445
           NLG+    L   FE+  Q VRMNYYPPC ++DKV+GLTPHSDA+              QI
Sbjct: 184 NLGVNPDKLASMFEDGTQGVRMNYYPPCVHADKVMGLTPHSDATGLTLLVQVNEVQGLQI 243

Query: 444 KKNGKWFPVKPI 409
           KKNGKW P+KP+
Sbjct: 244 KKNGKWVPIKPV 255



 Score = 86.3 bits (212), Expect(2) = 4e-55
 Identities = 39/72 (54%), Positives = 50/72 (69%)
 Frame = -2

Query: 329 YKSIEHRVVINPDKERLSIAVFHDPEYSAIIEPVQDLVKKLGARYKTVRHADYLNQSINN 150
           YKSIEHR V+NP+KERLSIA FH P++  +I P+ DL+K+  A YKT  H D+L   +  
Sbjct: 275 YKSIEHRAVVNPEKERLSIAAFHSPDHRTMIGPLPDLIKENSANYKTTSHDDFLRIVVTR 334

Query: 149 KLDSKTFLDQFK 114
           KLD K+ L Q K
Sbjct: 335 KLDGKSLLGQMK 346


>ref|XP_002527641.1| Flavonol synthase/flavanone 3-hydroxylase, putative [Ricinus
           communis] gi|223532946|gb|EEF34712.1| Flavonol
           synthase/flavanone 3-hydroxylase, putative [Ricinus
           communis]
          Length = 359

 Score =  160 bits (405), Expect(2) = 6e-54
 Identities = 80/195 (41%), Positives = 115/195 (58%)
 Frame = -1

Query: 984 IEKMKVDMEDFFRLPLEEKKVYGQLPDSTEGYGQSFVKSDEQKLEWADMHLLITXXXXXX 805
           +E MK+D+++FF+LP EEK  + QLP++ EGYGQ+FV SDEQKL+W DM  L+       
Sbjct: 96  MENMKMDIQEFFKLPFEEKMAFAQLPNNIEGYGQAFVVSDEQKLDWGDMLFLLPLPASSR 155

Query: 804 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNNPTSFSESVNKFSKDVQEVAMCLIGLMAR 625
                                          NPTSF E+ +K+S ++Q +A+C++ LMAR
Sbjct: 156 KMRFWP------------------------TNPTSFGETFDKYSSELQRIAVCILRLMAR 191

Query: 624 NLGLESQVLTKPFENCIQSVRMNYYPPCRYSDKVLGLTPHSDASSXXXXXXXXXXXXXQI 445
           NLG++ + +   FE+ +Q +RMNYYPPC  ++KV+GLT HSDA+              QI
Sbjct: 192 NLGIDPEDVATMFEDGVQGIRMNYYPPCIQANKVIGLTTHSDATGLTLLTQVNEVQGLQI 251

Query: 444 KKNGKWFPVKPILGA 400
           KK+G+W P+ PI GA
Sbjct: 252 KKDGRWVPITPIPGA 266



 Score = 77.8 bits (190), Expect(2) = 6e-54
 Identities = 34/74 (45%), Positives = 50/74 (67%)
 Frame = -2

Query: 329 YKSIEHRVVINPDKERLSIAVFHDPEYSAIIEPVQDLVKKLGARYKTVRHADYLNQSINN 150
           Y+SIEHR V+NP+KERLSIA FH+P+   +I P+ DLVK     YKT+ H +++   + +
Sbjct: 283 YRSIEHRAVVNPEKERLSIAAFHNPDIKTMIGPLGDLVKGKKPNYKTITHEEFVKLVVTS 342

Query: 149 KLDSKTFLDQFKLQ 108
           KLD K  +   K++
Sbjct: 343 KLDGKLLVGHMKVR 356


>emb|CAN69330.1| hypothetical protein VITISV_016336 [Vitis vinifera]
          Length = 344

 Score =  157 bits (398), Expect(2) = 2e-52
 Identities = 83/195 (42%), Positives = 114/195 (58%)
 Frame = -1

Query: 984 IEKMKVDMEDFFRLPLEEKKVYGQLPDSTEGYGQSFVKSDEQKLEWADMHLLITXXXXXX 805
           IEKMK D+++FF+LPL+EK  Y +LP   EGYGQ+FV S++QKL+WADMH L        
Sbjct: 83  IEKMKADVQEFFKLPLKEKNAYAKLPXGVEGYGQNFVVSZDQKLDWADMHFL-------- 134

Query: 804 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNNPTSFSESVNKFSKDVQEVAMCLIGLMAR 625
                                           PTSF E++ K+S ++ +V+ CL+ LMA+
Sbjct: 135 ----------------QXLPASERNMRFWPEEPTSFRETLEKYSAELVKVSNCLLKLMAK 178

Query: 624 NLGLESQVLTKPFENCIQSVRMNYYPPCRYSDKVLGLTPHSDASSXXXXXXXXXXXXXQI 445
           NL +  + LT  F++  Q+VRMNYYPPC ++ KV+GLTPHSD                QI
Sbjct: 179 NLLINPEQLTNMFDDGRQAVRMNYYPPCVHASKVIGLTPHSDFGGLTLLVQVNEXQGLQI 238

Query: 444 KKNGKWFPVKPILGA 400
           K+NGKW P++P+ GA
Sbjct: 239 KRNGKWIPIRPVPGA 253



 Score = 75.5 bits (184), Expect(2) = 2e-52
 Identities = 36/74 (48%), Positives = 50/74 (67%)
 Frame = -2

Query: 329 YKSIEHRVVINPDKERLSIAVFHDPEYSAIIEPVQDLVKKLGARYKTVRHADYLNQSINN 150
           YKSIEHR V++P+KERLSIA F  P   AII P+ +L K+ GA YK+V   +Y+   +  
Sbjct: 270 YKSIEHRAVVDPEKERLSIATFCSPNAGAIIGPLPELTKEKGAIYKSVSREEYIKFVLGR 329

Query: 149 KLDSKTFLDQFKLQ 108
           KLD K+ ++  KL+
Sbjct: 330 KLDGKSTINHMKLE 343


>ref|XP_002279610.1| PREDICTED: S-norcoclaurine synthase 1-like [Vitis vinifera]
          Length = 355

 Score =  149 bits (376), Expect(2) = 1e-51
 Identities = 79/192 (41%), Positives = 105/192 (54%)
 Frame = -1

Query: 984 IEKMKVDMEDFFRLPLEEKKVYGQLPDSTEGYGQSFVKSDEQKLEWADMHLLITXXXXXX 805
           I KM +D++DFF+L LEEK  Y QLP+  EGYGQ+FV S +QKL+W DM  L+       
Sbjct: 94  IVKMMMDVQDFFKLSLEEKNAYAQLPNDIEGYGQAFVVSRDQKLDWGDMLFLLPLPASQR 153

Query: 804 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNNPTSFSESVNKFSKDVQEVAMCLIGLMAR 625
                                           PTSF E++ K+S ++  V++ L+  MA+
Sbjct: 154 NMRLWP------------------------KKPTSFRETLEKYSSELHRVSINLLRSMAK 189

Query: 624 NLGLESQVLTKPFENCIQSVRMNYYPPCRYSDKVLGLTPHSDASSXXXXXXXXXXXXXQI 445
           NLG+    L   FE+  Q VRMNYYPPC  +DKV+GLTPHSDA+              QI
Sbjct: 190 NLGINPDKLATMFEDATQGVRMNYYPPCVNADKVMGLTPHSDATGLTLLVQVNEVQGLQI 249

Query: 444 KKNGKWFPVKPI 409
           KKNGKW P++P+
Sbjct: 250 KKNGKWVPIRPV 261



 Score = 81.3 bits (199), Expect(2) = 1e-51
 Identities = 37/72 (51%), Positives = 47/72 (65%)
 Frame = -2

Query: 329 YKSIEHRVVINPDKERLSIAVFHDPEYSAIIEPVQDLVKKLGARYKTVRHADYLNQSINN 150
           YKSIEHR V+NP+KERLSIA FH P Y  +I P+ DL+K+  A YKT+   D++   +  
Sbjct: 281 YKSIEHRAVVNPEKERLSIAAFHSPNYRTMIGPLPDLLKENSANYKTISFEDFVRIVVTR 340

Query: 149 KLDSKTFLDQFK 114
           KLD K  L   K
Sbjct: 341 KLDGKNLLGHMK 352