BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00000023
         (1919 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...   890   0.0  
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...   788   0.0  
dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       681   0.0  
dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]       649   0.0  
gb|ACO90219.1| stylopine synthase [Eschscholzia californica]          609   e-172

>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
            Full=(S)-tetrahydrocolumbamine oxidase
            (methylenedioxy-bridge-forming); AltName: Full=Cytochrome
            P450 719A1 gi|15822820|dbj|BAB68769.1| methylenedioxy
            bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score =  890 bits (2301), Expect = 0.0
 Identities = 455/491 (92%), Positives = 455/491 (92%)
 Frame = -1

Query: 1775 MEMNPLLXXXXXXXXXXXXXXIRILFSSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVA 1596
            MEMNPLL              IRILFSSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVA
Sbjct: 1    MEMNPLLVCATVAIVFATTTIIRILFSSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVA 60

Query: 1595 LAKLAKVHGSVMTIWIGSWRPVIVISDIEKAWEVLVNKSADYGARDMPEITKIASASWHT 1416
            LAKLAKVHGSVMTIWIGSWRPVIVISDIEKAWEVLVNKSADYGARDMPEITKIASASWHT
Sbjct: 61   LAKLAKVHGSVMTIWIGSWRPVIVISDIEKAWEVLVNKSADYGARDMPEITKIASASWHT 120

Query: 1415 ISTSDAGSFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLD 1236
            ISTSDAGSFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLD
Sbjct: 121  ISTSDAGSFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLD 180

Query: 1235 HIKKNTVRLLTRLIFGQAFDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKA 1056
            HIKKNTVRLLTRLIFGQAFDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKA
Sbjct: 181  HIKKNTVRLLTRLIFGQAFDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKA 240

Query: 1055 EREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQNXXXXXXXXXXXELYLLGVDSTSST 876
            EREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQN           ELYLLGVDSTSST
Sbjct: 241  EREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQNFEEEVIIFCIFELYLLGVDSTSST 300

Query: 875  TTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLKYLQGVVKETMRMKPIAPLA 696
            TTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLKYLQGVVKETMRMKPIAPLA
Sbjct: 301  TTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLKYLQGVVKETMRMKPIAPLA 360

Query: 695  IPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPYKFMPERFLEGETGTAYNKAM 516
            IPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPYKFMPERFLEGETGTAYNKAM
Sbjct: 361  IPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPYKFMPERFLEGETGTAYNKAM 420

Query: 515  EQSFLPFSAGMRICAGMDLGKLQXXXXXXXXXXXFKWSCVEEGKLPDMGEELSFVLLMKT 336
            EQSFLPFSAGMRICAGMDLGKLQ           FKWSCVEEGKLPDMGEELSFVLLMKT
Sbjct: 421  EQSFLPFSAGMRICAGMDLGKLQFAFALANLVNAFKWSCVEEGKLPDMGEELSFVLLMKT 480

Query: 335  PLEARIAGRNV 303
            PLEARIAGRNV
Sbjct: 481  PLEARIAGRNV 491


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score =  788 bits (2034), Expect = 0.0
 Identities = 392/463 (84%), Positives = 418/463 (90%)
 Frame = -1

Query: 1697 SSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVIS 1518
            SSSS  QMKWP+GP KLPIIGNLHQLG DVLHVALAKLA+VHGSVMT+WIG+WRP+IVIS
Sbjct: 28   SSSSFSQMKWPTGPNKLPIIGNLHQLGGDVLHVALAKLAQVHGSVMTVWIGNWRPIIVIS 87

Query: 1517 DIEKAWEVLVNKSADYGARDMPEITKIASASWHTISTSDAGSFWQNVRKGLQSGAMGPLN 1338
            DI+KAWEVLVNKS+DYGARDMPEITKIASASWHTISTSDAG+FWQ VRKGLQSGAMGPLN
Sbjct: 88   DIDKAWEVLVNKSSDYGARDMPEITKIASASWHTISTSDAGAFWQTVRKGLQSGAMGPLN 147

Query: 1337 VAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQAFDDNKFIE 1158
            +AAQ+Q+QERDMKRLI+A+ DEAA NN IVKPLDHIKKNTVRLLTRLIFGQ FDD+KF+E
Sbjct: 148  IAAQSQFQERDMKRLIQALRDEAAQNNNIVKPLDHIKKNTVRLLTRLIFGQTFDDDKFVE 207

Query: 1157 SMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPPTN 978
            SMH+EI+DIIRISGYARLAEAFYYAKYLPSHKKAEREA+LVKCRVE+LV PLLSS PPTN
Sbjct: 208  SMHFEIDDIIRISGYARLAEAFYYAKYLPSHKKAEREAYLVKCRVEDLVSPLLSSNPPTN 267

Query: 977  SYLYFLLSQNXXXXXXXXXXXELYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTL 798
            SYLYFLLSQ            ELYLLGVDSTSSTTTWALAYLIREQ  QEKLYQD RMTL
Sbjct: 268  SYLYFLLSQRFSEEVIIFCIFELYLLGVDSTSSTTTWALAYLIREQAIQEKLYQDTRMTL 327

Query: 797  GDVDLVKIEDVNKLKYLQGVVKETMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNL 618
            GDVDLVKIEDV+KLKYLQ VVKETMRMKPIAPLAIPHKTAK+TTLMG+KVAKGTRIMVNL
Sbjct: 328  GDVDLVKIEDVSKLKYLQAVVKETMRMKPIAPLAIPHKTAKDTTLMGSKVAKGTRIMVNL 387

Query: 617  YALHHNQNIWPDPYKFMPERFLEGETGTAYNKAMEQSFLPFSAGMRICAGMDLGKLQXXX 438
            YALHHNQNIW +PYKFMPERFL+GE G+A NKAMEQSFLPFSAGMRICAGMDLGKLQ   
Sbjct: 388  YALHHNQNIWTNPYKFMPERFLQGEDGSASNKAMEQSFLPFSAGMRICAGMDLGKLQFAF 447

Query: 437  XXXXXXXXFKWSCVEEGKLPDMGEELSFVLLMKTPLEARIAGR 309
                    FKWSCVEEGK PDM EELSFVLLMKTPLEA+I  R
Sbjct: 448  ALANLVNAFKWSCVEEGKFPDMSEELSFVLLMKTPLEAKITAR 490


>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  681 bits (1756), Expect = 0.0
 Identities = 339/465 (72%), Positives = 382/465 (82%)
 Frame = -1

Query: 1697 SSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVIS 1518
            SSSS  + KWPSGP++LPIIGNLH LG D+LHV LAKLAKVHGSVMTIWIG WRP+IVIS
Sbjct: 27   SSSSSSKKKWPSGPKRLPIIGNLHLLGGDLLHVTLAKLAKVHGSVMTIWIGRWRPIIVIS 86

Query: 1517 DIEKAWEVLVNKSADYGARDMPEITKIASASWHTISTSDAGSFWQNVRKGLQSGAMGPLN 1338
            DI  AWEVLV+KSA+YG RD P+I K  +   + IS SD G FW NVRKGLQ+GA+ PLN
Sbjct: 87   DINSAWEVLVSKSAEYGQRDTPDIFKTLTVGQNNISMSDIGPFWHNVRKGLQNGALSPLN 146

Query: 1337 VAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQAFDDNKFIE 1158
            VAAQ Q++E D+KRLI  MS EA N NGI+KPLDH+KKNT RLL RLIFG  FD++KF+E
Sbjct: 147  VAAQTQFREEDVKRLIHEMSREALNKNGIIKPLDHMKKNTARLLGRLIFGPTFDNDKFVE 206

Query: 1157 SMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPPTN 978
            S+ YEIED++R+SGYARL EAFYYAKYLPS KK  REA+L K R+EEL+RPLLSS PPTN
Sbjct: 207  SVLYEIEDVLRLSGYARLTEAFYYAKYLPSQKKVAREAYLAKYRIEELMRPLLSSNPPTN 266

Query: 977  SYLYFLLSQNXXXXXXXXXXXELYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTL 798
            SYLYFL SQN           EL+LLGVDSTSST TWALAYLI EQ  QEKL +DIRMT+
Sbjct: 267  SYLYFLQSQNYSKEIIIFCTFELFLLGVDSTSSTATWALAYLIHEQAVQEKLNEDIRMTI 326

Query: 797  GDVDLVKIEDVNKLKYLQGVVKETMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNL 618
            GDVDLVKIED++KLKYLQ VVKETMRMKPIAPLAIPHKTA +TTLMGTKVA+GT+IMVNL
Sbjct: 327  GDVDLVKIEDLSKLKYLQAVVKETMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNL 386

Query: 617  YALHHNQNIWPDPYKFMPERFLEGETGTAYNKAMEQSFLPFSAGMRICAGMDLGKLQXXX 438
            YALHHN++IWP+PYKFMPERFL+GE  +A NKAMEQSFLPF AGMRICAGMDLGKLQ   
Sbjct: 387  YALHHNEDIWPEPYKFMPERFLQGENVSANNKAMEQSFLPFGAGMRICAGMDLGKLQIAF 446

Query: 437  XXXXXXXXFKWSCVEEGKLPDMGEELSFVLLMKTPLEARIAGRNV 303
                    FKWSCV+EGKLPDM E+L  V LMKTPLE RI+ R V
Sbjct: 447  ALANLVNAFKWSCVDEGKLPDMSEQLFAVTLMKTPLEGRISTRKV 491


>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 508

 Score =  649 bits (1675), Expect = 0.0
 Identities = 335/499 (67%), Positives = 386/499 (77%), Gaps = 5/499 (1%)
 Frame = -1

Query: 1784 IAEMEMNPLLXXXXXXXXXXXXXXIRILFS-SSSLPQMKWPSGPRKLPIIGNLHQLGDDV 1608
            +AEME + LL              +RI  S SSS  QM WPSGP+KLPIIGNLHQL  ++
Sbjct: 10   VAEMEGSLLLAGATLAILLTISTIVRITSSPSSSFSQMTWPSGPKKLPIIGNLHQLRGEL 69

Query: 1607 LHVALAKLAKVHGSVMTIWIGSWRPVIVISDIEKAWEVLVNKSADYGARDMP---EITKI 1437
            LHV LAKLAKVHG VMTIW+GSW P+IVISDI  AWEVLVNKSADYGAR++    E+ + 
Sbjct: 70   LHVTLAKLAKVHGGVMTIWMGSWVPIIVISDINSAWEVLVNKSADYGAREVTYLHELLRD 129

Query: 1436 ASASWHTISTSDAGSFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAMSDEAANNN 1257
            ++   H IS+SD G  W N+RKGLQSGA+ PL+VA+Q Q++ERDMKR++  MS E  NNN
Sbjct: 130  SAMHPHNISSSDVGPLWHNIRKGLQSGALSPLSVASQTQHRERDMKRVVHEMSREIVNNN 189

Query: 1256 GIVKPLDHIKKNTVRLLTRLIFGQAFDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKY 1077
            G +KPL HIK +T RLL RLIFG +F+D+KFIESM YEIED IR S Y  L  +FYYAKY
Sbjct: 190  GTIKPLVHIKNSTARLLGRLIFGPSFNDSKFIESMLYEIEDFIRTSSYTSLTNSFYYAKY 249

Query: 1076 LPSHKKAEREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQNXXXXXXXXXXXELYLLG 897
            LPS +KA REA L K R+EELVRPLLSSKPPTN+YLYFLLSQN           EL+LLG
Sbjct: 250  LPSQRKATREAKLGKYRIEELVRPLLSSKPPTNAYLYFLLSQNLSEEVTIFCIFELFLLG 309

Query: 896  VDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLKYLQGVVKETMRM 717
            VDS SSTTTWALAY+IREQ AQEKLYQDIRMT+GDVDLVKIEDVNKLKYLQ VVKETMRM
Sbjct: 310  VDSASSTTTWALAYMIREQEAQEKLYQDIRMTIGDVDLVKIEDVNKLKYLQSVVKETMRM 369

Query: 716  KPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPYKFMPERFLEGETG 537
            KPIAPLA+PH   K+TTLMGTK+ KGTR+MVNLYALHH +++W +P KFMPER+L+GE G
Sbjct: 370  KPIAPLALPHNAGKDTTLMGTKIVKGTRVMVNLYALHHYEDVWLEPSKFMPERYLQGEDG 429

Query: 536  TAYNKAMEQSFLPFSAGMRICAGMDLGKLQXXXXXXXXXXXFKWSCVEEGKLPDMGEEL- 360
            +  +KAMEQS LPF AGMRICAGMDLGKLQ           FKWSCVE+GKLPDM EEL 
Sbjct: 430  SVTHKAMEQSLLPFGAGMRICAGMDLGKLQVAFVLANLVNAFKWSCVEDGKLPDMTEELF 489

Query: 359  SFVLLMKTPLEARIAGRNV 303
              ++LMKTPLE RI+ R V
Sbjct: 490  GLIILMKTPLEGRISPRTV 508


>gb|ACO90219.1| stylopine synthase [Eschscholzia californica]
          Length = 495

 Score =  609 bits (1571), Expect = e-172
 Identities = 299/468 (63%), Positives = 366/468 (78%), Gaps = 2/468 (0%)
 Frame = -1

Query: 1703 LFSSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIV 1524
            LF +    +M WP+GP+ LPIIGN+H LG   L V L  LAKVHGSVMTIWIGSWRPVIV
Sbjct: 28   LFFTRKSSKMVWPAGPKTLPIIGNMHLLGGTALQVVLHNLAKVHGSVMTIWIGSWRPVIV 87

Query: 1523 ISDIEKAWEVLVNKSADYGARDMPEITKIASASWHTISTSDAGSFWQNVRKGLQSGAMGP 1344
            +SDIE+AWEVLVNKS+DY ARDMP+ITKI SA W TISTSD+G  W N+RKGLQ+ A+ P
Sbjct: 88   VSDIERAWEVLVNKSSDYSARDMPDITKIISADWKTISTSDSGPHWTNLRKGLQNVALSP 147

Query: 1343 LNVAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQAFDDNKF 1164
             N+AAQ Q+QE+DM ++I+ + +EA NNNGIVKPLDH+KK T+RL++RL+FGQ F+++K+
Sbjct: 148  HNLAAQFQFQEKDMTKMIQTLQEEARNNNGIVKPLDHMKKATLRLISRLVFGQDFNNDKY 207

Query: 1163 IESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPP 984
            ++ MH  IE++IR+SGYARLAEAFYYAKYLPSHKKA RE    + RV+ LV P LS  PP
Sbjct: 208  VDDMHLAIEELIRVSGYARLAEAFYYAKYLPSHKKAVREVEEAQRRVQNLVSPFLSLNPP 267

Query: 983  TNSYLYFLLSQNXXXXXXXXXXXELYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRM 804
            TN+YL+FL SQ            E YLLGVDSTS TT WALA+LIRE   QEKLYQ++  
Sbjct: 268  TNTYLHFLRSQKYDDEVIIFAIFEAYLLGVDSTSLTTAWALAFLIRESNVQEKLYQELES 327

Query: 803  TLGDVD--LVKIEDVNKLKYLQGVVKETMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRI 630
                +D  ++K+ED+NKL+YLQ V+KETMRMKPIAPLAIPHK  ++T+LMG K+ KGTR+
Sbjct: 328  FASKMDRKILKVEDINKLQYLQAVIKETMRMKPIAPLAIPHKACRDTSLMGKKIDKGTRV 387

Query: 629  MVNLYALHHNQNIWPDPYKFMPERFLEGETGTAYNKAMEQSFLPFSAGMRICAGMDLGKL 450
            MVN+YALHHN+N++ DP+KFMPERF++ ++  A  KAMEQS LPFSAGMRICAGM+LGKL
Sbjct: 388  MVNIYALHHNKNVFNDPFKFMPERFMKVDSQDANGKAMEQSLLPFSAGMRICAGMELGKL 447

Query: 449  QXXXXXXXXXXXFKWSCVEEGKLPDMGEELSFVLLMKTPLEARIAGRN 306
            Q           FKWSCV +G LPDM ++L FVLLMKTPLEARI  RN
Sbjct: 448  QFSFALANLAYAFKWSCVADGVLPDMSDQLGFVLLMKTPLEARINRRN 495