BLASTX nr result
ID: Cocculus23_contig00062123
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cocculus23_contig00062123
(276 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003633777.1| PREDICTED: putative pentatricopeptide repeat... 122 7e-26
emb|CBI21281.3| unnamed protein product [Vitis vinifera] 122 7e-26
ref|XP_007032019.1| Pentatricopeptide repeat (PPR) superfamily p... 120 3e-25
ref|XP_006468886.1| PREDICTED: putative pentatricopeptide repeat... 114 1e-23
ref|XP_006446942.1| hypothetical protein CICLE_v10014272mg [Citr... 114 1e-23
ref|XP_002512846.1| pentatricopeptide repeat-containing protein,... 114 1e-23
gb|EXB62840.1| hypothetical protein L484_008690 [Morus notabilis] 113 3e-23
ref|XP_007217844.1| hypothetical protein PRUPE_ppa024283mg [Prun... 110 2e-22
ref|XP_006405473.1| hypothetical protein EUTSA_v10027641mg [Eutr... 110 3e-22
ref|XP_002885940.1| pentatricopeptide repeat-containing protein ... 108 1e-21
ref|XP_006292165.1| hypothetical protein CARUB_v10018371mg, part... 107 2e-21
gb|EYU39774.1| hypothetical protein MIMGU_mgv1a023460mg, partial... 105 5e-21
ref|XP_003624492.1| Pentatricopeptide repeat-containing protein ... 105 5e-21
ref|NP_186850.1| pentatricopeptide repeat-containing protein [Ar... 105 7e-21
ref|XP_004493071.1| PREDICTED: putative pentatricopeptide repeat... 104 1e-20
ref|XP_007161628.1| hypothetical protein PHAVU_001G085300g [Phas... 102 4e-20
gb|AAU90328.2| Pentatricopeptide repeat domain containing protei... 102 4e-20
ref|XP_003553304.1| PREDICTED: putative pentatricopeptide repeat... 102 6e-20
ref|XP_006353946.1| PREDICTED: putative pentatricopeptide repeat... 102 7e-20
ref|XP_002303012.2| pentatricopeptide repeat-containing family p... 102 7e-20
>ref|XP_003633777.1| PREDICTED: putative pentatricopeptide repeat-containing protein
At2g01510-like, partial [Vitis vinifera]
Length = 825
Score = 122 bits (305), Expect = 7e-26
Identities = 59/92 (64%), Positives = 71/92 (77%)
Frame = -1
Query: 276 QKAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSL 97
++A V ADQATF KQLHSFII+SGFMSNVF+GSAL+DVYA CGS+
Sbjct: 444 RQASVIADQATFASLLRASASIASLSLGKQLHSFIIKSGFMSNVFSGSALLDVYAKCGSI 503
Query: 96 RDAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
+DA+QTF+ MPDRNIVSWNAMI+AYA+NG+ E
Sbjct: 504 KDAVQTFQEMPDRNIVSWNAMISAYAQNGEAE 535
Score = 57.0 bits (136), Expect = 3e-06
Identities = 26/61 (42%), Positives = 44/61 (72%)
Frame = -1
Query: 192 KQLHSFIIRSGFMSNVFAGSALVDVYASCGSLRDAIQTFKGMPDRNIVSWNAMIAAYARN 13
+Q+HSF+I++ F+ NVF +AL+D Y+ S+ DA + F MP+++ VS+N +I+ YA +
Sbjct: 270 QQIHSFVIKTNFVWNVFVSNALLDFYSKHDSVIDARKLFDEMPEQDGVSYNVIISGYAWD 329
Query: 12 G 10
G
Sbjct: 330 G 330
>emb|CBI21281.3| unnamed protein product [Vitis vinifera]
Length = 785
Score = 122 bits (305), Expect = 7e-26
Identities = 59/92 (64%), Positives = 71/92 (77%)
Frame = -1
Query: 276 QKAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSL 97
++A V ADQATF KQLHSFII+SGFMSNVF+GSAL+DVYA CGS+
Sbjct: 404 RQASVIADQATFASLLRASASIASLSLGKQLHSFIIKSGFMSNVFSGSALLDVYAKCGSI 463
Query: 96 RDAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
+DA+QTF+ MPDRNIVSWNAMI+AYA+NG+ E
Sbjct: 464 KDAVQTFQEMPDRNIVSWNAMISAYAQNGEAE 495
Score = 57.0 bits (136), Expect = 3e-06
Identities = 26/61 (42%), Positives = 44/61 (72%)
Frame = -1
Query: 192 KQLHSFIIRSGFMSNVFAGSALVDVYASCGSLRDAIQTFKGMPDRNIVSWNAMIAAYARN 13
+Q+HSF+I++ F+ NVF +AL+D Y+ S+ DA + F MP+++ VS+N +I+ YA +
Sbjct: 230 QQIHSFVIKTNFVWNVFVSNALLDFYSKHDSVIDARKLFDEMPEQDGVSYNVIISGYAWD 289
Query: 12 G 10
G
Sbjct: 290 G 290
>ref|XP_007032019.1| Pentatricopeptide repeat (PPR) superfamily protein [Theobroma
cacao] gi|508711048|gb|EOY02945.1| Pentatricopeptide
repeat (PPR) superfamily protein [Theobroma cacao]
Length = 818
Score = 120 bits (300), Expect = 3e-25
Identities = 58/91 (63%), Positives = 70/91 (76%)
Frame = -1
Query: 273 KAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSLR 94
KA V ADQATF KQLHSF+IR+GFMSNVF+GSAL+D+YA CGS++
Sbjct: 438 KAGVRADQATFASMLKASANLASLSLGKQLHSFVIRTGFMSNVFSGSALLDMYAKCGSIK 497
Query: 93 DAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
DAIQ F+ MP+RNIVSWNA+I+AYA+NGDGE
Sbjct: 498 DAIQLFRDMPERNIVSWNALISAYAQNGDGE 528
>ref|XP_006468886.1| PREDICTED: putative pentatricopeptide repeat-containing protein
At2g01510-like [Citrus sinensis]
Length = 828
Score = 114 bits (285), Expect = 1e-23
Identities = 56/91 (61%), Positives = 68/91 (74%)
Frame = -1
Query: 273 KAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSLR 94
+A + ADQATF KQLHSF+IRSGFMSNVF+GSAL+D+YA GSL+
Sbjct: 448 RANISADQATFASILRASAELASLSLGKQLHSFVIRSGFMSNVFSGSALLDMYAKSGSLK 507
Query: 93 DAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
DAIQTFK MP+RNIVSWNA+I+A A+NGD +
Sbjct: 508 DAIQTFKEMPERNIVSWNALISACAQNGDAQ 538
>ref|XP_006446942.1| hypothetical protein CICLE_v10014272mg [Citrus clementina]
gi|557549553|gb|ESR60182.1| hypothetical protein
CICLE_v10014272mg [Citrus clementina]
Length = 830
Score = 114 bits (285), Expect = 1e-23
Identities = 56/91 (61%), Positives = 68/91 (74%)
Frame = -1
Query: 273 KAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSLR 94
+A + ADQATF KQLHSF+IRSGFMSNVF+GSAL+D+YA GSL+
Sbjct: 450 RANISADQATFASILRASAELASLSLGKQLHSFVIRSGFMSNVFSGSALLDMYAKSGSLK 509
Query: 93 DAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
DAIQTFK MP+RNIVSWNA+I+A A+NGD +
Sbjct: 510 DAIQTFKEMPERNIVSWNALISACAQNGDAQ 540
>ref|XP_002512846.1| pentatricopeptide repeat-containing protein, putative [Ricinus
communis] gi|223547857|gb|EEF49349.1| pentatricopeptide
repeat-containing protein, putative [Ricinus communis]
Length = 553
Score = 114 bits (285), Expect = 1e-23
Identities = 53/92 (57%), Positives = 69/92 (75%)
Frame = -1
Query: 276 QKAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSL 97
++ V ADQATF KQLHS++IRSGF SNV++GSALVD+YA CGS+
Sbjct: 438 RRTNVSADQATFASVLKASANLASISLGKQLHSYLIRSGFWSNVYSGSALVDMYAKCGSV 497
Query: 96 RDAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
++AI+TFK MP RN++SWNA+I+AYA+NGDGE
Sbjct: 498 KEAIRTFKEMPGRNVISWNALISAYAQNGDGE 529
>gb|EXB62840.1| hypothetical protein L484_008690 [Morus notabilis]
Length = 741
Score = 113 bits (282), Expect = 3e-23
Identities = 53/92 (57%), Positives = 68/92 (73%)
Frame = -1
Query: 276 QKAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSL 97
++ VCADQATF +QLHS +IRSGFMSNVF+GSAL+D+YA CG +
Sbjct: 428 RRESVCADQATFASVLRASASLALLSLGRQLHSSLIRSGFMSNVFSGSALLDMYAKCGFM 487
Query: 96 RDAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
+DA+QTF+ MP RN VSWNA+I+AYA+NGDG+
Sbjct: 488 KDALQTFQEMPKRNSVSWNALISAYAQNGDGQ 519
Score = 55.8 bits (133), Expect = 6e-06
Identities = 24/60 (40%), Positives = 41/60 (68%)
Frame = -1
Query: 189 QLHSFIIRSGFMSNVFAGSALVDVYASCGSLRDAIQTFKGMPDRNIVSWNAMIAAYARNG 10
Q+H+ +++SG S +F ++L+D Y G + A+Q FK +PDR+ V++NA+I YA+ G
Sbjct: 154 QVHAHVVKSGCDSVLFVCNSLIDSYCKTGRIELALQVFKEIPDRDSVTFNALITGYAKEG 213
>ref|XP_007217844.1| hypothetical protein PRUPE_ppa024283mg [Prunus persica]
gi|462413994|gb|EMJ19043.1| hypothetical protein
PRUPE_ppa024283mg [Prunus persica]
Length = 717
Score = 110 bits (275), Expect = 2e-22
Identities = 53/92 (57%), Positives = 66/92 (71%)
Frame = -1
Query: 276 QKAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSL 97
++A V DQATF KQLHS +IR GF SNVFAGSAL+D+YA CGS+
Sbjct: 336 RRANVSPDQATFASILRASANLASLSLGKQLHSSVIRLGFASNVFAGSALLDMYAKCGSM 395
Query: 96 RDAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
+DA+QTF+ MP RN+V WNA+I+AYA+NGDGE
Sbjct: 396 KDALQTFQEMPKRNLVCWNALISAYAQNGDGE 427
>ref|XP_006405473.1| hypothetical protein EUTSA_v10027641mg [Eutrema salsugineum]
gi|557106611|gb|ESQ46926.1| hypothetical protein
EUTSA_v10027641mg [Eutrema salsugineum]
Length = 824
Score = 110 bits (274), Expect = 3e-22
Identities = 52/90 (57%), Positives = 65/90 (72%)
Frame = -1
Query: 270 AEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSLRD 91
A + ADQ+TF KQLH+FIIRSG + NVF+GS LVD+YA CGS++D
Sbjct: 445 ASLRADQSTFATVLRASASLASLSLGKQLHAFIIRSGNLENVFSGSGLVDMYAKCGSIKD 504
Query: 90 AIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
A+Q F+ MPDRN+VSWNA+I+AYA NGDGE
Sbjct: 505 AVQVFREMPDRNVVSWNALISAYADNGDGE 534
>ref|XP_002885940.1| pentatricopeptide repeat-containing protein [Arabidopsis lyrata
subsp. lyrata] gi|297331780|gb|EFH62199.1|
pentatricopeptide repeat-containing protein [Arabidopsis
lyrata subsp. lyrata]
Length = 944
Score = 108 bits (269), Expect = 1e-21
Identities = 52/90 (57%), Positives = 64/90 (71%)
Frame = -1
Query: 270 AEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSLRD 91
A + ADQ+TF KQLH+FIIRSG + NVF+GS LVD+YA CGS++D
Sbjct: 440 ANLRADQSTFATVLKASAGFASLLLGKQLHAFIIRSGNLENVFSGSGLVDMYAKCGSIKD 499
Query: 90 AIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
A+Q F+ MPDRN VSWNA+I+AYA NGDGE
Sbjct: 500 AVQVFEEMPDRNAVSWNALISAYADNGDGE 529
>ref|XP_006292165.1| hypothetical protein CARUB_v10018371mg, partial [Capsella rubella]
gi|482560872|gb|EOA25063.1| hypothetical protein
CARUB_v10018371mg, partial [Capsella rubella]
Length = 849
Score = 107 bits (266), Expect = 2e-21
Identities = 52/90 (57%), Positives = 62/90 (68%)
Frame = -1
Query: 270 AEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSLRD 91
A + ADQ+TF KQLH FIIRSG + NVF+GS LVD+YA CGS++D
Sbjct: 470 ANLRADQSTFATVLKACASFASLLLGKQLHGFIIRSGNLENVFSGSGLVDMYAKCGSIKD 529
Query: 90 AIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
A+Q F MPDRN VSWNA+I+AYA NGDGE
Sbjct: 530 AVQVFAEMPDRNAVSWNALISAYADNGDGE 559
>gb|EYU39774.1| hypothetical protein MIMGU_mgv1a023460mg, partial [Mimulus
guttatus]
Length = 722
Score = 105 bits (263), Expect = 5e-21
Identities = 50/90 (55%), Positives = 62/90 (68%)
Frame = -1
Query: 276 QKAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSL 97
++ VC DQATF KQ HS II SGF+SN+F GSAL+D+YA CGSL
Sbjct: 341 RRNNVCGDQATFASTLRASANLALLSLGKQFHSCIIGSGFLSNIFCGSALLDMYAKCGSL 400
Query: 96 RDAIQTFKGMPDRNIVSWNAMIAAYARNGD 7
+DAI F+ MP+RN +SWNAMI+AYA+NGD
Sbjct: 401 KDAISIFRHMPERNSISWNAMISAYAQNGD 430
>ref|XP_003624492.1| Pentatricopeptide repeat-containing protein [Medicago truncatula]
gi|355499507|gb|AES80710.1| Pentatricopeptide
repeat-containing protein [Medicago truncatula]
Length = 1125
Score = 105 bits (263), Expect = 5e-21
Identities = 51/92 (55%), Positives = 67/92 (72%)
Frame = -1
Query: 276 QKAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSL 97
Q+A++ AD AT+ KQLHS II SG++SNVF+GSALVD+YA CGS+
Sbjct: 493 QRAKIGADAATYASIVRACASLASLTLGKQLHSHIIGSGYISNVFSGSALVDMYAKCGSI 552
Query: 96 RDAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
+DA+Q F+ MP RN VSWNA+I+AYA+NGDG+
Sbjct: 553 KDALQMFQEMPVRNSVSWNALISAYAQNGDGD 584
>ref|NP_186850.1| pentatricopeptide repeat-containing protein [Arabidopsis thaliana]
gi|75193830|sp|Q9S7F4.1|PP206_ARATH RecName:
Full=Putative pentatricopeptide repeat-containing
protein At2g01510 gi|6091739|gb|AAF03451.1|AC010797_27
hypothetical protein [Arabidopsis thaliana]
gi|6513930|gb|AAF14834.1|AC011664_16 hypothetical
protein [Arabidopsis thaliana]
gi|332640228|gb|AEE73749.1| pentatricopeptide
repeat-containing protein [Arabidopsis thaliana]
Length = 825
Score = 105 bits (262), Expect = 7e-21
Identities = 50/86 (58%), Positives = 62/86 (72%)
Frame = -1
Query: 258 ADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSLRDAIQT 79
ADQ+TF KQLH+FIIRSG + NVF+GS LVD+YA CGS++DA+Q
Sbjct: 450 ADQSTFATVLKASASFASLLLGKQLHAFIIRSGNLENVFSGSGLVDMYAKCGSIKDAVQV 509
Query: 78 FKGMPDRNIVSWNAMIAAYARNGDGE 1
F+ MPDRN VSWNA+I+A+A NGDGE
Sbjct: 510 FEEMPDRNAVSWNALISAHADNGDGE 535
>ref|XP_004493071.1| PREDICTED: putative pentatricopeptide repeat-containing protein
At2g01510-like [Cicer arietinum]
Length = 812
Score = 104 bits (260), Expect = 1e-20
Identities = 50/92 (54%), Positives = 67/92 (72%)
Frame = -1
Query: 276 QKAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSL 97
Q+A++ AD AT+ KQLHS I RSG++SNVF+GSAL+D+YA CGS+
Sbjct: 431 QRAKIGADAATYASIVRACASLASLTLGKQLHSHINRSGYISNVFSGSALLDMYAKCGSI 490
Query: 96 RDAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
+DA+Q F+ MP RN VSWNA+I+AYA+NGDG+
Sbjct: 491 KDALQMFQEMPVRNSVSWNALISAYAQNGDGD 522
>ref|XP_007161628.1| hypothetical protein PHAVU_001G085300g [Phaseolus vulgaris]
gi|561035092|gb|ESW33622.1| hypothetical protein
PHAVU_001G085300g [Phaseolus vulgaris]
Length = 862
Score = 102 bits (255), Expect = 4e-20
Identities = 49/91 (53%), Positives = 65/91 (71%)
Frame = -1
Query: 276 QKAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSL 97
Q+A++ AD AT+ KQLHS IIRSG +SNVF+GSALVD+YA CGS+
Sbjct: 481 QRAKIRADSATYASILRACANLASLTLGKQLHSHIIRSGCISNVFSGSALVDMYAKCGSI 540
Query: 96 RDAIQTFKGMPDRNIVSWNAMIAAYARNGDG 4
++A+ F+ MP RN V+WNA+I+AYA+NGDG
Sbjct: 541 KEALHMFQEMPTRNCVTWNALISAYAQNGDG 571
>gb|AAU90328.2| Pentatricopeptide repeat domain containing protein, putative
[Solanum demissum]
Length = 819
Score = 102 bits (255), Expect = 4e-20
Identities = 49/91 (53%), Positives = 63/91 (69%)
Frame = -1
Query: 273 KAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSLR 94
+ V DQATF KQLHS +IR G +S+VF+GS LVD+YA+CGS++
Sbjct: 439 RENVHGDQATFASTLKASANLASVSLGKQLHSSVIRLGLLSSVFSGSVLVDMYANCGSMK 498
Query: 93 DAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
DAI+ FK MPDRNIV WNA+I+AY++NGD E
Sbjct: 499 DAIEVFKEMPDRNIVCWNALISAYSQNGDAE 529
>ref|XP_003553304.1| PREDICTED: putative pentatricopeptide repeat-containing protein
At2g01510-like [Glycine max]
Length = 815
Score = 102 bits (254), Expect = 6e-20
Identities = 50/90 (55%), Positives = 65/90 (72%)
Frame = -1
Query: 273 KAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSLR 94
+A++ AD AT+ KQLHS IIRSG +SNVF+GSALVD+YA CGS++
Sbjct: 435 RAKIGADSATYASILRACANLASLTLGKQLHSRIIRSGCLSNVFSGSALVDMYAKCGSIK 494
Query: 93 DAIQTFKGMPDRNIVSWNAMIAAYARNGDG 4
+A+Q F+ MP RN VSWNA+I+AYA+NGDG
Sbjct: 495 EALQMFQEMPVRNSVSWNALISAYAQNGDG 524
>ref|XP_006353946.1| PREDICTED: putative pentatricopeptide repeat-containing protein
At2g01510-like [Solanum tuberosum]
Length = 819
Score = 102 bits (253), Expect = 7e-20
Identities = 49/91 (53%), Positives = 63/91 (69%)
Frame = -1
Query: 273 KAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSLR 94
+ V DQATF KQLHS +IR G +S+VF+GS LVD+YA+CGS++
Sbjct: 439 RENVHGDQATFASTLKASANLASVSLGKQLHSSVIRLGLLSSVFSGSVLVDMYANCGSMK 498
Query: 93 DAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
DAI+ FK MPDRNIV WNA+I+AYA+NG+ E
Sbjct: 499 DAIEVFKEMPDRNIVCWNALISAYAQNGNAE 529
>ref|XP_002303012.2| pentatricopeptide repeat-containing family protein [Populus
trichocarpa] gi|550345695|gb|EEE82285.2|
pentatricopeptide repeat-containing family protein
[Populus trichocarpa]
Length = 814
Score = 102 bits (253), Expect = 7e-20
Identities = 49/92 (53%), Positives = 68/92 (73%)
Frame = -1
Query: 276 QKAEVCADQATFXXXXXXXXXXXXXXXSKQLHSFIIRSGFMSNVFAGSALVDVYASCGSL 97
++A V ADQATF KQLHS +IRSGFM NV++G ALVD+YA+C S+
Sbjct: 434 RRANVSADQATFACVLKASANLASILLGKQLHSCVIRSGFM-NVYSGCALVDMYANCASI 492
Query: 96 RDAIQTFKGMPDRNIVSWNAMIAAYARNGDGE 1
+DAI+TF+ M +RN+V+WNA+++AYA+NGDG+
Sbjct: 493 KDAIKTFEEMSERNVVTWNALLSAYAQNGDGK 524