BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cocculus23_contig00060081
         (305 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_007010661.1| FAD-binding Berberine family protein [Theobr...   118   1e-24
ref|XP_003633008.1| PREDICTED: LOW QUALITY PROTEIN: reticuline o...   117   2e-24
ref|XP_003633009.1| PREDICTED: LOW QUALITY PROTEIN: reticuline o...   116   4e-24
ref|XP_007038641.1| FAD-binding Berberine family protein [Theobr...   112   7e-23
ref|XP_006370482.1| FAD-binding domain-containing family protein...   112   7e-23
ref|XP_006356501.1| PREDICTED: cannabidiolic acid synthase-like ...   111   9e-23
ref|XP_002523148.1| Reticuline oxidase precursor, putative [Rici...   111   9e-23
ref|XP_007038638.1| FAD-binding Berberine family protein [Theobr...   110   2e-22
ref|XP_007045724.1| FAD-binding Berberine family protein [Theobr...   109   5e-22
gb|AGL44335.1| FAD-dependent oxidoreductase [Papaver somniferum]      108   6e-22
ref|XP_004242239.1| PREDICTED: cannabidiolic acid synthase-like ...   108   6e-22
ref|XP_002523149.1| Reticuline oxidase precursor, putative [Rici...   108   6e-22
gb|EYU19096.1| hypothetical protein MIMGU_mgv1a004131mg [Mimulus...   108   1e-21
ref|XP_006377943.1| hypothetical protein POPTR_0011s16540g [Popu...   107   1e-21
emb|CAN60568.1| hypothetical protein VITISV_000225 [Vitis vinifera]   107   1e-21
ref|XP_004242238.1| PREDICTED: cannabidiolic acid synthase-like ...   107   2e-21
ref|XP_007023038.1| FAD-binding Berberine family protein [Theobr...   106   3e-21
ref|XP_007023036.1| FAD-binding Berberine family protein [Theobr...   106   3e-21
ref|XP_002267029.2| PREDICTED: reticuline oxidase-like protein-l...   106   3e-21
emb|CBI31059.3| unnamed protein product [Vitis vinifera]              105   5e-21

>ref|XP_007010661.1| FAD-binding Berberine family protein [Theobroma cacao]
           gi|508727574|gb|EOY19471.1| FAD-binding Berberine family
           protein [Theobroma cacao]
          Length = 576

 Score =  118 bits (295), Expect = 1e-24
 Identities = 55/101 (54%), Positives = 78/101 (77%), Gaps = 1/101 (0%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP 126
           +E K T+QAAF ++F GGV+ L+ +ME RFPEL V ++DC+EM++IQS +YL +FPI+ P
Sbjct: 340 QEGKDTIQAAFTALFLGGVDNLISLMEIRFPELGVVKQDCIEMSWIQSLLYLDQFPIERP 399

Query: 125 ELLLQRNQ-SRRFFKAKSDYVKEPISEIGLKGIWERFYEIE 6
           E+LL R   ++  FK KSDYVKEPI +I  +G+W++FYE E
Sbjct: 400 EILLDRTAVNKTLFKVKSDYVKEPIPKIVFEGMWQKFYEEE 440


>ref|XP_003633008.1| PREDICTED: LOW QUALITY PROTEIN: reticuline oxidase-like
           protein-like [Vitis vinifera]
          Length = 499

 Score =  117 bits (293), Expect = 2e-24
 Identities = 60/102 (58%), Positives = 80/102 (78%), Gaps = 2/102 (1%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP 126
           +E K T++A+F S+F GGV++LLP+M + FPEL + +EDC+EM++I+S +Y A FP  A 
Sbjct: 254 EEGKKTIEASFNSLFLGGVDELLPLMXESFPELGLVKEDCIEMSWIESILYFAGFPGGAS 313

Query: 125 -ELLLQRNQS-RRFFKAKSDYVKEPISEIGLKGIWERFYEIE 6
            ++LL R  S RRFFKAKSD+VKEPISEI L+GIW RFYE E
Sbjct: 314 LDVLLDRTPSPRRFFKAKSDHVKEPISEIRLEGIWRRFYEEE 355


>ref|XP_003633009.1| PREDICTED: LOW QUALITY PROTEIN: reticuline oxidase-like
           protein-like [Vitis vinifera]
          Length = 538

 Score =  116 bits (290), Expect = 4e-24
 Identities = 60/103 (58%), Positives = 81/103 (78%), Gaps = 2/103 (1%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP 126
           +E K T++A+F S+F GGV++LLP+M++ FPEL + +EDC+EM++I+S +Y A FP  A 
Sbjct: 299 EEGKKTIEASFNSLFLGGVDELLPLMQESFPELGLVKEDCIEMSWIESILYFAGFPGGAS 358

Query: 125 -ELLLQRNQ-SRRFFKAKSDYVKEPISEIGLKGIWERFYEIEV 3
            + LL R   +RRFFKAKSDYVKEPISEIGL+GI  RFYE +V
Sbjct: 359 LDGLLDRTPLTRRFFKAKSDYVKEPISEIGLEGIXRRFYEEDV 401


>ref|XP_007038641.1| FAD-binding Berberine family protein [Theobroma cacao]
           gi|508775886|gb|EOY23142.1| FAD-binding Berberine family
           protein [Theobroma cacao]
          Length = 532

 Score =  112 bits (279), Expect = 7e-23
 Identities = 50/101 (49%), Positives = 75/101 (74%), Gaps = 1/101 (0%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP 126
           +E K T+QA F S+F GG ++L  +ME+RFPEL + +++C+EM++IQS +Y ++FP++  
Sbjct: 296 QEGKETIQAVFRSLFLGGADKLFSLMEKRFPELGLVKQECIEMSWIQSHLYFSDFPLERS 355

Query: 125 ELLLQRNQ-SRRFFKAKSDYVKEPISEIGLKGIWERFYEIE 6
           E+LL R   ++ F K KSDYVKEPI +I  +G+W RFYE E
Sbjct: 356 EILLDRTAFTKGFIKVKSDYVKEPIPKIAFEGLWRRFYEEE 396


>ref|XP_006370482.1| FAD-binding domain-containing family protein [Populus trichocarpa]
           gi|550349675|gb|ERP67051.1| FAD-binding
           domain-containing family protein [Populus trichocarpa]
          Length = 527

 Score =  112 bits (279), Expect = 7e-23
 Identities = 54/102 (52%), Positives = 80/102 (78%), Gaps = 2/102 (1%)
 Frame = -1

Query: 302 ESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP- 126
           +S  T+QA+F S+F GG+++LLP+++  FPEL + +EDC+EM++IQS +Y   FP ++  
Sbjct: 290 KSNTTIQASFNSLFLGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYFDGFPSNSSL 349

Query: 125 ELLLQRNQS-RRFFKAKSDYVKEPISEIGLKGIWERFYEIEV 3
           ++LL R  S RR FKAKSDYVKEPI E+GL+GIWERF++ ++
Sbjct: 350 DVLLDRTPSTRRNFKAKSDYVKEPIPELGLEGIWERFFDKDI 391


>ref|XP_006356501.1| PREDICTED: cannabidiolic acid synthase-like [Solanum tuberosum]
          Length = 538

 Score =  111 bits (278), Expect = 9e-23
 Identities = 55/97 (56%), Positives = 76/97 (78%), Gaps = 2/97 (2%)
 Frame = -1

Query: 290 TVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP-ELLL 114
           T+ A+F S+F GG+++LLPIM++ FPEL ++REDC+EM++I+S +Y A FP D P ++LL
Sbjct: 300 TIVASFNSIFLGGIDRLLPIMKENFPELGLRREDCIEMSWIESIMYFAGFPTDGPLDVLL 359

Query: 113 QRNQ-SRRFFKAKSDYVKEPISEIGLKGIWERFYEIE 6
            R Q S R+FKAKSDYV +PI E GL+GIW  F+E E
Sbjct: 360 SRVQLSTRYFKAKSDYVYQPIPEGGLEGIWRFFFEDE 396


>ref|XP_002523148.1| Reticuline oxidase precursor, putative [Ricinus communis]
           gi|223537555|gb|EEF39179.1| Reticuline oxidase
           precursor, putative [Ricinus communis]
          Length = 442

 Score =  111 bits (278), Expect = 9e-23
 Identities = 56/102 (54%), Positives = 77/102 (75%), Gaps = 1/102 (0%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP 126
           ++ K TV+AAF S+F GGV++LLP+M++ FPEL + REDC+EM++I+S +Y A F     
Sbjct: 296 QQGKTTVRAAFNSLFLGGVDRLLPLMQESFPELGLVREDCIEMSWIESILYFAGFSNSPL 355

Query: 125 ELLLQRNQ-SRRFFKAKSDYVKEPISEIGLKGIWERFYEIEV 3
           ++LL R Q S R FKAKSDYVKEP+ E  L+GIWER  E++V
Sbjct: 356 DILLNRTQPSVRNFKAKSDYVKEPMPETALEGIWERLSEVDV 397


>ref|XP_007038638.1| FAD-binding Berberine family protein [Theobroma cacao]
           gi|508775883|gb|EOY23139.1| FAD-binding Berberine family
           protein [Theobroma cacao]
          Length = 532

 Score =  110 bits (275), Expect = 2e-22
 Identities = 51/101 (50%), Positives = 75/101 (74%), Gaps = 1/101 (0%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP 126
           +E K T+QA F ++F GGV+ L+ +ME+RFPEL V ++DC+EM+ I+S +Y  +FP++  
Sbjct: 296 QEGKDTIQAVFAALFLGGVDNLVSLMEKRFPELGVVKQDCIEMSCIESFLYFGQFPLERL 355

Query: 125 ELLLQRNQ-SRRFFKAKSDYVKEPISEIGLKGIWERFYEIE 6
           E+LL R   ++  FK KSDYVKEPI +I  +G+W+RFYE E
Sbjct: 356 EILLDRTAVNKTLFKVKSDYVKEPIPKIVFEGMWQRFYEEE 396


>ref|XP_007045724.1| FAD-binding Berberine family protein [Theobroma cacao]
           gi|508709659|gb|EOY01556.1| FAD-binding Berberine family
           protein [Theobroma cacao]
          Length = 542

 Score =  109 bits (272), Expect = 5e-22
 Identities = 53/100 (53%), Positives = 73/100 (73%), Gaps = 2/100 (2%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP 126
           +E K T+QA+F+S+F G  +QL+ +M++ FPEL + +EDC EM +I+S +Y AE P  +P
Sbjct: 304 QEGKRTIQASFVSMFLGRTDQLVKLMQENFPELRLVKEDCHEMNWIESIIYFAEIPNSSP 363

Query: 125 -ELLLQRN-QSRRFFKAKSDYVKEPISEIGLKGIWERFYE 12
            + LL RN     FFKAKSDYVKEPI E  L+G+W+RFYE
Sbjct: 364 LDALLDRNLYPETFFKAKSDYVKEPIPETALEGLWQRFYE 403


>gb|AGL44335.1| FAD-dependent oxidoreductase [Papaver somniferum]
          Length = 535

 Score =  108 bits (271), Expect = 6e-22
 Identities = 50/99 (50%), Positives = 74/99 (74%), Gaps = 1/99 (1%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP 126
           KE + T+ A+F S++ G  E LL +M++RFPEL ++ +DC EMT+IQSQ+Y+  FP++  
Sbjct: 301 KEGQKTILASFPSLYLGNTENLLAVMKERFPELGLETKDCAEMTWIQSQLYINGFPVNGS 360

Query: 125 -ELLLQRNQSRRFFKAKSDYVKEPISEIGLKGIWERFYE 12
            ++LL RNQ +R+ K KSDYVKEPI E GL+G+W++  E
Sbjct: 361 LDILLSRNQVKRYAKIKSDYVKEPIPETGLEGLWKKILE 399


>ref|XP_004242239.1| PREDICTED: cannabidiolic acid synthase-like [Solanum lycopersicum]
          Length = 538

 Score =  108 bits (271), Expect = 6e-22
 Identities = 54/97 (55%), Positives = 74/97 (76%), Gaps = 2/97 (2%)
 Frame = -1

Query: 290 TVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP-ELLL 114
           T+ A+F S+F GG+++LLPIM++ FPEL ++R DC+EM++I+S +Y A FP D P ++LL
Sbjct: 300 TIVASFNSIFLGGIDRLLPIMQENFPELGLRRADCIEMSWIESILYFAGFPTDGPLDVLL 359

Query: 113 QRNQ-SRRFFKAKSDYVKEPISEIGLKGIWERFYEIE 6
            R Q S R+FKAKSDYV +PI E GL GIW  F+E E
Sbjct: 360 SRVQLSTRYFKAKSDYVYQPIPEGGLDGIWRFFFEDE 396


>ref|XP_002523149.1| Reticuline oxidase precursor, putative [Ricinus communis]
           gi|223537556|gb|EEF39180.1| Reticuline oxidase
           precursor, putative [Ricinus communis]
          Length = 469

 Score =  108 bits (271), Expect = 6e-22
 Identities = 55/102 (53%), Positives = 76/102 (74%), Gaps = 1/102 (0%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP 126
           ++ K TV+AAF S+F GGV++LLP+M++ FPEL + REDC+ M++I+S +Y A F     
Sbjct: 233 QQGKTTVRAAFNSLFLGGVDRLLPLMQESFPELGLVREDCINMSWIESILYFAGFSNSPL 292

Query: 125 ELLLQRNQ-SRRFFKAKSDYVKEPISEIGLKGIWERFYEIEV 3
           ++LL R Q S R FKAKSDYVKEP+ E  L+GIWER  E++V
Sbjct: 293 DILLSRTQPSVRNFKAKSDYVKEPMPETALEGIWERLSEMDV 334


>gb|EYU19096.1| hypothetical protein MIMGU_mgv1a004131mg [Mimulus guttatus]
          Length = 543

 Score =  108 bits (269), Expect = 1e-21
 Identities = 53/96 (55%), Positives = 72/96 (75%), Gaps = 1/96 (1%)
 Frame = -1

Query: 290 TVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAPELLLQ 111
           T+ A+F S+F GGV++LLP+M++ FPEL + REDC EM++I+S +Y   FPI+  E+LL 
Sbjct: 308 TLNASFNSIFLGGVDRLLPMMQESFPELGLVREDCTEMSWIESILYFDGFPIENREVLLN 367

Query: 110 RNQ-SRRFFKAKSDYVKEPISEIGLKGIWERFYEIE 6
           R Q S R+FKAKSDYV+ PI E  L+GIW  F+E E
Sbjct: 368 RTQRSVRYFKAKSDYVQTPIPEFALEGIWRLFFEPE 403


>ref|XP_006377943.1| hypothetical protein POPTR_0011s16540g [Populus trichocarpa]
           gi|550328548|gb|ERP55740.1| hypothetical protein
           POPTR_0011s16540g [Populus trichocarpa]
          Length = 536

 Score =  107 bits (268), Expect = 1e-21
 Identities = 52/103 (50%), Positives = 75/103 (72%), Gaps = 2/103 (1%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP 126
           +E K T+ AAF S+F GGV++LLP+M++ FPEL + +EDC+EM++I+S +Y+  FP +A 
Sbjct: 299 EEGKTTILAAFTSLFLGGVDRLLPLMQESFPELGLVKEDCIEMSWIKSVLYVVGFPSNAS 358

Query: 125 E--LLLQRNQSRRFFKAKSDYVKEPISEIGLKGIWERFYEIEV 3
              LL +   + R FK KSDYVKEP+ E  L+GIWERF E ++
Sbjct: 359 SDVLLARTPLTNRNFKGKSDYVKEPMPETALEGIWERFLEADI 401


>emb|CAN60568.1| hypothetical protein VITISV_000225 [Vitis vinifera]
          Length = 171

 Score =  107 bits (268), Expect = 1e-21
 Identities = 54/94 (57%), Positives = 76/94 (80%), Gaps = 2/94 (2%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP 126
           +E K T++A+F S+F GGV++LLP+M++ FPEL + +EDC+EM++I+S +Y A FP  A 
Sbjct: 78  EEGKKTIEASFNSLFLGGVDELLPLMQESFPELGLVKEDCIEMSWIESILYFAGFPSGAS 137

Query: 125 -ELLLQRNQ-SRRFFKAKSDYVKEPISEIGLKGI 30
            ++LL R   ++RFFKAKSDYVKEPISEIGL+GI
Sbjct: 138 LDVLLDRTPLTQRFFKAKSDYVKEPISEIGLEGI 171


>ref|XP_004242238.1| PREDICTED: cannabidiolic acid synthase-like [Solanum lycopersicum]
          Length = 536

 Score =  107 bits (267), Expect = 2e-21
 Identities = 54/100 (54%), Positives = 77/100 (77%), Gaps = 2/100 (2%)
 Frame = -1

Query: 299 SKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPIDAP-E 123
           +K T+ A+F S+F GG+++LLPIM++ FPEL +++EDC+EM++IQS +Y A FPI    +
Sbjct: 293 NKQTIVASFNSLFIGGIDRLLPIMQENFPELGLRKEDCIEMSWIQSIMYFAGFPIHGSLD 352

Query: 122 LLLQRNQ-SRRFFKAKSDYVKEPISEIGLKGIWERFYEIE 6
           +LL R Q + R+FKAKSDYV +PI E GL+GIW  F+E E
Sbjct: 353 VLLSRVQPTTRYFKAKSDYVYQPIPEGGLEGIWRFFFEEE 392


>ref|XP_007023038.1| FAD-binding Berberine family protein [Theobroma cacao]
           gi|508778404|gb|EOY25660.1| FAD-binding Berberine family
           protein [Theobroma cacao]
          Length = 529

 Score =  106 bits (265), Expect = 3e-21
 Identities = 51/101 (50%), Positives = 74/101 (73%), Gaps = 1/101 (0%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFP-IDA 129
           +E K TVQA+F S+F G ++QL+ +M++ FPE  + +EDC+EM++IQS +Y AE P  ++
Sbjct: 292 QEGKRTVQASFTSLFLGRIDQLIALMQESFPEFGLVKEDCIEMSWIQSILYFAEIPQSES 351

Query: 128 PELLLQRNQSRRFFKAKSDYVKEPISEIGLKGIWERFYEIE 6
            ++LL R  +  FFK KSDYV EPI EI L+G+W+ FYE E
Sbjct: 352 LDVLLNRTVTAGFFKGKSDYVTEPIPEIALEGLWQWFYEDE 392


>ref|XP_007023036.1| FAD-binding Berberine family protein [Theobroma cacao]
           gi|508778402|gb|EOY25658.1| FAD-binding Berberine family
           protein [Theobroma cacao]
          Length = 528

 Score =  106 bits (265), Expect = 3e-21
 Identities = 49/99 (49%), Positives = 73/99 (73%), Gaps = 1/99 (1%)
 Frame = -1

Query: 305 KESKGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFP-IDA 129
           +E   T+QA+F S+F G ++QL+P+M++ FPEL + +EDC+EM++IQS +Y A  P  ++
Sbjct: 292 QEGNRTIQASFTSLFLGRIDQLIPLMQESFPELGLVKEDCIEMSWIQSILYFAGIPQSES 351

Query: 128 PELLLQRNQSRRFFKAKSDYVKEPISEIGLKGIWERFYE 12
            ++LL R  +  FFK KSDYV EPI EI L+G+W+ FYE
Sbjct: 352 LDVLLNRTDTASFFKGKSDYVTEPIPEIALEGLWQWFYE 390


>ref|XP_002267029.2| PREDICTED: reticuline oxidase-like protein-like [Vitis vinifera]
          Length = 780

 Score =  106 bits (265), Expect = 3e-21
 Identities = 52/100 (52%), Positives = 77/100 (77%), Gaps = 2/100 (2%)
 Frame = -1

Query: 296 KGTVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPI-DAPEL 120
           K T+QA+F S+F GGV++L+P+M + FPEL ++  DC EMT+I+S +Y A FP  ++ ++
Sbjct: 541 KMTIQASFNSLFLGGVDKLIPLMGKSFPELGLQAGDCTEMTWIESVLYFAGFPRGNSLDV 600

Query: 119 LLQR-NQSRRFFKAKSDYVKEPISEIGLKGIWERFYEIEV 3
           LL R +  R +FKAKSDYVKEPI E+GL+G+WERF + ++
Sbjct: 601 LLNRTHPDRSYFKAKSDYVKEPIPEVGLEGVWERFLKEQI 640


>emb|CBI31059.3| unnamed protein product [Vitis vinifera]
          Length = 421

 Score =  105 bits (263), Expect = 5e-21
 Identities = 51/98 (52%), Positives = 76/98 (77%), Gaps = 2/98 (2%)
 Frame = -1

Query: 290 TVQAAFLSVFQGGVEQLLPIMEQRFPELSVKREDCMEMTYIQSQVYLAEFPI-DAPELLL 114
           T+QA+F S+F GGV++L+P+M + FPEL ++  DC EMT+I+S +Y A FP  ++ ++LL
Sbjct: 184 TIQASFNSLFLGGVDKLIPLMGKSFPELGLQAGDCTEMTWIESVLYFAGFPRGNSLDVLL 243

Query: 113 QR-NQSRRFFKAKSDYVKEPISEIGLKGIWERFYEIEV 3
            R +  R +FKAKSDYVKEPI E+GL+G+WERF + ++
Sbjct: 244 NRTHPDRSYFKAKSDYVKEPIPEVGLEGVWERFLKEQI 281