BLASTX nr result
ID: Cocculus23_contig00048361
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cocculus23_contig00048361
(625 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransf... 117 8e-40
ref|XP_002278149.1| PREDICTED: tabersonine 16-O-methyltransferas... 69 9e-23
ref|XP_006377431.1| 6a-hydroxymaackiain methyltransferase family... 67 8e-22
emb|CAN73269.1| hypothetical protein VITISV_021917 [Vitis vinifera] 65 1e-21
ref|XP_002525160.1| o-methyltransferase, putative [Ricinus commu... 74 2e-21
ref|XP_006474725.1| PREDICTED: trans-resveratrol di-O-methyltran... 72 5e-21
ref|XP_006346269.1| PREDICTED: trans-resveratrol di-O-methyltran... 72 5e-21
ref|XP_006346268.1| PREDICTED: trans-resveratrol di-O-methyltran... 72 5e-21
ref|XP_006452811.1| hypothetical protein CICLE_v10008718mg [Citr... 73 6e-21
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 70 1e-20
ref|XP_002278127.1| PREDICTED: isoflavone-7-O-methyltransferase ... 70 1e-20
ref|XP_002525159.1| o-methyltransferase, putative [Ricinus commu... 73 2e-20
emb|CAN75970.1| hypothetical protein VITISV_009881 [Vitis vinifera] 70 2e-20
gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 67 3e-20
ref|XP_003633246.1| PREDICTED: tabersonine 16-O-methyltransferas... 69 4e-20
ref|XP_006364329.1| PREDICTED: uncharacterized protein LOC102587... 65 7e-20
ref|XP_006364318.1| PREDICTED: trans-resveratrol di-O-methyltran... 68 7e-20
ref|XP_002305104.2| 6a-hydroxymaackiain methyltransferase family... 63 1e-19
ref|XP_002281368.2| PREDICTED: 8-hydroxyquercetin 8-O-methyltran... 64 2e-19
ref|XP_002517827.1| o-methyltransferase, putative [Ricinus commu... 72 2e-19
>sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransferase; Short=CoOMT;
AltName: Full=Tetrahydrocolumbamine
2-O-methyltransferase gi|24210466|dbj|BAC22084.1|
columbamine O-methyltransferase [Coptis japonica]
Length = 351
Score = 117 bits (294), Expect(2) = 8e-40
Identities = 71/156 (45%), Positives = 95/156 (60%)
Frame = +2
Query: 8 MRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEFARW 187
MRY+VHM LFT+ T +EDK Y LTP SK+L+ H++SLAP +MLQ EF+ W
Sbjct: 73 MRYLVHMQLFTISTDQITKEDK--YELTPASKLLVHGHQKSLAPYVMLQTH--PEEFSVW 128
Query: 188 AHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGLKRG 367
+H+I LDG K +WES+ YE E DP+ N+ + + MT+ + M+ ALV GL +
Sbjct: 129 SHVINVLDG--KKPYWESN-DTSMYEKTEGDPEINEILNDAMTSHSTFMLPALVSGLMKE 185
Query: 368 KVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
V+D V S+VDVGGN G VAK I+ AF
Sbjct: 186 NVLDG---------VASIVDVGGNSGVVAKGIVDAF 212
Score = 72.8 bits (177), Expect(2) = 8e-40
Identities = 33/54 (61%), Positives = 42/54 (77%)
Frame = +1
Query: 463 IKCVPHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHNCE 624
+ PH+KC+VMDL HVI+ V K+PKLD+VA DMF SIP+ D +LLK+ LHN E
Sbjct: 209 VDAFPHVKCSVMDLNHVIERVIKNPKLDYVAGDMFTSIPNADAILLKSTLHNYE 262
>ref|XP_002278149.1| PREDICTED: tabersonine 16-O-methyltransferase isoform 1 [Vitis
vinifera]
Length = 357
Score = 68.9 bits (167), Expect(2) = 9e-23
Identities = 49/159 (30%), Positives = 73/159 (45%), Gaps = 1/159 (0%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQ-EDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEF 178
++MR +VH F + AD E + Y LT S+ LLKD S APLL++Q D
Sbjct: 72 RLMRVLVHSGFFA--QQQADHNEQEQLYSLTYASRFLLKDEPTSGAPLLLVQVDP---HL 126
Query: 179 ARWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGL 358
H + +S T + + YG PF++ +PK+N M + + + +V
Sbjct: 127 TNPCHFLSDWFRNSDPTPFVTAYGKPFWDYAAHEPKFNNFFNEAMASDSQLIASVVVGEC 186
Query: 359 KRGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
K R ++SL+DVGG G +AK I AF
Sbjct: 187 KE-----------VFRGLSSLIDVGGGTGTMAKVIAKAF 214
Score = 64.3 bits (155), Expect(2) = 9e-23
Identities = 28/51 (54%), Positives = 36/51 (70%)
Frame = +1
Query: 466 KCVPHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
K PHLKCTV D PHV+ N+ L+FV D+F++IP D +LLK+ILHN
Sbjct: 212 KAFPHLKCTVFDQPHVVANLQGGENLEFVGGDIFEAIPPADAILLKSILHN 262
>ref|XP_006377431.1| 6a-hydroxymaackiain methyltransferase family protein [Populus
trichocarpa] gi|550327721|gb|ERP55228.1|
6a-hydroxymaackiain methyltransferase family protein
[Populus trichocarpa]
Length = 358
Score = 67.4 bits (163), Expect(2) = 8e-22
Identities = 52/160 (32%), Positives = 86/160 (53%), Gaps = 2/160 (1%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQED-KDYYGLTPVSKILLKDHKRSLAPLLMLQADD-LQNE 175
++MR +VH F I +QE+ ++ Y LT SK+LLKD SL+P+++ D L N
Sbjct: 72 RLMRVLVHSGFFDTIKIHDNQEEVEEGYVLTSSSKLLLKDSPTSLSPVVLTMLDPVLMNP 131
Query: 176 FARWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDG 355
W L E + G +T +E+++G+ F E +++ K+ + GMT+ + +V +V
Sbjct: 132 ---WFSLGEWIQGKE-RTPFETYHGMSFGEYGKRNFKFINNLNEGMTSDSK-LVSLVVK- 185
Query: 356 LKRGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
EH+ +V SLVDVGG G +A++I A+
Sbjct: 186 ----------EHKEIFESVDSLVDVGGGTGTLARSIADAY 215
Score = 62.8 bits (151), Expect(2) = 8e-22
Identities = 26/48 (54%), Positives = 36/48 (75%)
Frame = +1
Query: 475 PHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
PH+KCTV+DLP V+ N+P+ L FV DMF SIPS D +++K++L N
Sbjct: 216 PHMKCTVLDLPQVVANLPESENLKFVGGDMFKSIPSADAIIIKSVLLN 263
>emb|CAN73269.1| hypothetical protein VITISV_021917 [Vitis vinifera]
Length = 357
Score = 65.5 bits (158), Expect(2) = 1e-21
Identities = 48/159 (30%), Positives = 72/159 (45%), Gaps = 1/159 (0%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQ-EDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEF 178
++MR +V F + AD E + Y LT S+ LLKD S APLL++Q D
Sbjct: 72 RLMRVLVXSGFFA--QQQADHNEQEQLYSLTYASRFLLKDEPTSGAPLLLVQVDP---HL 126
Query: 179 ARWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGL 358
H + +S T + + YG PF++ +PK+N M + + + +V
Sbjct: 127 TNPCHFLSDWFRNSDPTPFVTAYGKPFWDYAAHEPKFNNFFNEAMASDSQLIASVVVGEC 186
Query: 359 KRGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
K R ++SL+DVGG G +AK I AF
Sbjct: 187 KE-----------VFRGLSSLIDVGGGTGTMAKVIAKAF 214
Score = 64.3 bits (155), Expect(2) = 1e-21
Identities = 28/51 (54%), Positives = 36/51 (70%)
Frame = +1
Query: 466 KCVPHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
K PHLKCTV D PHV+ N+ L+FV D+F++IP D +LLK+ILHN
Sbjct: 212 KAFPHLKCTVFDQPHVVANLQGGENLEFVGGDIFEAIPPADAILLKSILHN 262
>ref|XP_002525160.1| o-methyltransferase, putative [Ricinus communis]
gi|223535619|gb|EEF37287.1| o-methyltransferase,
putative [Ricinus communis]
Length = 356
Score = 73.6 bits (179), Expect(2) = 2e-21
Identities = 48/158 (30%), Positives = 75/158 (47%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEFA 181
++MR ++H F K ++++++ Y LT SK+LLKD+ S+AP L+ D +
Sbjct: 71 RLMRILIHSGFFAK-AKIEEKDEEEGYVLTNASKLLLKDNPLSIAPFLLAMLDPIL--IK 127
Query: 182 RWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGLK 361
W +L + F +H G+ F+E DP N M + ++ L+D
Sbjct: 128 PWHYLSTWFQNDDATAFDTAH-GMGFWEYTASDPNLNNFFNEAMASDARLVMKVLID--- 183
Query: 362 RGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
EH+G + SLVDVGG G +A AI AF
Sbjct: 184 --------EHKGLFEGLKSLVDVGGGTGTMAAAIAKAF 213
Score = 55.5 bits (132), Expect(2) = 2e-21
Identities = 26/50 (52%), Positives = 33/50 (66%)
Frame = +1
Query: 466 KCVPHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILH 615
K P L+CTV DLPHV+ + L +VA DMF++IP D VLLK I+H
Sbjct: 211 KAFPQLECTVFDLPHVVVGLQGTENLKYVAGDMFEAIPPADAVLLKWIMH 260
>ref|XP_006474725.1| PREDICTED: trans-resveratrol di-O-methyltransferase-like [Citrus
sinensis]
Length = 365
Score = 72.4 bits (176), Expect(2) = 5e-21
Identities = 51/159 (32%), Positives = 79/159 (49%), Gaps = 1/159 (0%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADD-LQNEF 178
++MR +VH F + KT++ E ++ Y LT SK+LLKDH SLAP L+ D L +
Sbjct: 76 RLMRILVHSGFFA-LQKTSENEQEEGYILTSASKLLLKDHPLSLAPFLLAMLDPILTKPW 134
Query: 179 ARWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGL 358
+ + I+ D + T + +G+ F++ ++ + N GM + T AL+
Sbjct: 135 HQVSTWIQNDDDDDAATTFALPHGMNFWDYAVRETRLNHFFNEGMASDTRLTSSALIHKC 194
Query: 359 KRGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
K D EG + +LVDVGG G +A AI F
Sbjct: 195 K-------DVFEG----LNTLVDVGGGTGTLASAIAKKF 222
Score = 55.1 bits (131), Expect(2) = 5e-21
Identities = 23/51 (45%), Positives = 35/51 (68%)
Frame = +1
Query: 466 KCVPHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
K PH++CTV D PHV+ ++ + L +V +MF++IP D VL+K +LHN
Sbjct: 220 KKFPHIECTVFDQPHVVADLKSNGNLKYVGGNMFEAIPPADAVLIKCVLHN 270
>ref|XP_006346269.1| PREDICTED: trans-resveratrol di-O-methyltransferase-like [Solanum
tuberosum]
Length = 364
Score = 72.4 bits (176), Expect(2) = 5e-21
Identities = 48/159 (30%), Positives = 78/159 (49%), Gaps = 1/159 (0%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQAD-DLQNEF 178
++MR +VH F QE ++ Y LTP S++LLKD S+ P L Q D +L + +
Sbjct: 80 RLMRILVHAGFFI-------QEAEEGYLLTPTSRLLLKDEPMSMIPFLNFQLDPNLMDPW 132
Query: 179 ARWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGL 358
+ + S+ T + + +G+PF++ E +P+ N M + T ++ L+
Sbjct: 133 HSLSKWFNNVSDDSNTTPYATAHGMPFFKYAENEPRLNHLFNEAMASDTRLVMTVLIQNG 192
Query: 359 KRGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
K G + + + SLVDVGG G +AKAI F
Sbjct: 193 K-GLIFEG---------LKSLVDVGGGTGTIAKAIADTF 221
Score = 55.1 bits (131), Expect(2) = 5e-21
Identities = 25/48 (52%), Positives = 33/48 (68%)
Frame = +1
Query: 475 PHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
P + CTV +LPHVI+ + L FV DMF+SIPS + +LLK ILH+
Sbjct: 222 PQINCTVFELPHVIEGLKGSKNLSFVGGDMFNSIPSANAILLKWILHD 269
>ref|XP_006346268.1| PREDICTED: trans-resveratrol di-O-methyltransferase-like [Solanum
tuberosum]
Length = 353
Score = 71.6 bits (174), Expect(2) = 5e-21
Identities = 55/158 (34%), Positives = 79/158 (50%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEFA 181
++MR ++H + F E +D Y LTP S++LLK+ SLAP + Q D + +
Sbjct: 75 RLMRILIHASFFI--------EGEDGYLLTPTSRLLLKNEPLSLAPFVQAQLDPILMD-- 124
Query: 182 RWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGLK 361
W L E S F +H G P ++ DP+ N+ M + ++ AL+ K
Sbjct: 125 PWHSLGEWFANGDSTPFATAH-GKPLFDYAGDDPRLNRIFNEAMESDARLIMSALI---K 180
Query: 362 RGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
GK + EG +TSLVDVGG G VAKAI +AF
Sbjct: 181 NGKRV----FEG----LTSLVDVGGGIGTVAKAISNAF 210
Score = 55.8 bits (133), Expect(2) = 5e-21
Identities = 24/48 (50%), Positives = 33/48 (68%)
Frame = +1
Query: 475 PHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
P LKC+V DLPHV++ + L ++ DMF S+PS D +LLK ILH+
Sbjct: 211 PELKCSVFDLPHVVEGLEGGNNLSYITGDMFKSVPSADAILLKWILHD 258
>ref|XP_006452811.1| hypothetical protein CICLE_v10008718mg [Citrus clementina]
gi|557556037|gb|ESR66051.1| hypothetical protein
CICLE_v10008718mg [Citrus clementina]
Length = 365
Score = 72.8 bits (177), Expect(2) = 6e-21
Identities = 51/158 (32%), Positives = 79/158 (50%), Gaps = 1/158 (0%)
Frame = +2
Query: 5 IMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADD-LQNEFA 181
+MR +VH F + KT++ E ++ Y LT SK+LLKDH SLAP L+ D L +
Sbjct: 77 LMRILVHSGFFA-LQKTSENEQEEGYILTSASKLLLKDHPLSLAPFLLAMLDPILTKPWH 135
Query: 182 RWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGLK 361
+ + I+ D + T + +G+ F++ ++ + N GM + T AL++ K
Sbjct: 136 QVSTWIQNDDDDDAATTFALPHGMNFWDYAVRETRLNHFFNEGMASDTRLTSSALINKCK 195
Query: 362 RGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
D EG + +LVDVGG G +A AI F
Sbjct: 196 -------DVFEG----LNTLVDVGGGTGTLASAIAKKF 222
Score = 54.3 bits (129), Expect(2) = 6e-21
Identities = 23/51 (45%), Positives = 35/51 (68%)
Frame = +1
Query: 466 KCVPHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
K PH++CTV D PHV+ ++ + L +V +MF++IP D VL+K +LHN
Sbjct: 220 KKFPHIECTVFDQPHVVADLKSNGYLKYVGGNMFEAIPPADAVLIKCVLHN 270
>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 2 [Papaver somniferum]
gi|571330882|gb|AHF27397.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'- O-methyltransferase 2 [synthetic construct]
Length = 357
Score = 70.1 bits (170), Expect(2) = 1e-20
Identities = 47/159 (29%), Positives = 81/159 (50%), Gaps = 1/159 (0%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEF- 178
+++RY+VH+N+ + +T + + Y L PV +LL+D +RS+ P+++ Q +F
Sbjct: 76 RMVRYLVHLNI--IEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMT---QKDFM 130
Query: 179 ARWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGL 358
W + EGL G+ S T +E G+ ++ +E +P +Q GM T + L++
Sbjct: 131 VSWHFMKEGL-GNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDC 189
Query: 359 KRGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
+ T + + SLVD+GG G KAI AF
Sbjct: 190 R-----------DTFQGLDSLVDIGGGNGTTIKAIYEAF 217
Score = 56.2 bits (134), Expect(2) = 1e-20
Identities = 25/48 (52%), Positives = 33/48 (68%)
Frame = +1
Query: 475 PHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
PH+KCT+ DLPHV+ N P ++ V DMF S+PS +LLK ILH+
Sbjct: 218 PHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQAILLKLILHD 265
>ref|XP_002278127.1| PREDICTED: isoflavone-7-O-methyltransferase 9 [Vitis vinifera]
Length = 354
Score = 70.5 bits (171), Expect(2) = 1e-20
Identities = 29/48 (60%), Positives = 37/48 (77%)
Frame = +1
Query: 475 PHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
PHLKCTV+DLP V+ N+PK LD+V DMF SIPS D + +K++LHN
Sbjct: 212 PHLKCTVLDLPQVVANLPKSENLDYVGGDMFQSIPSADAIFIKSVLHN 259
Score = 55.8 bits (133), Expect(2) = 1e-20
Identities = 47/158 (29%), Positives = 75/158 (47%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEFA 181
Q+MR +VH F + +QE Y LTP S++L+K + SLAP+++ D +
Sbjct: 72 QLMRLLVHSGFFAMQKVDENQEG---YVLTPPSRLLVKGNATSLAPIVLGMLDPVL--VT 126
Query: 182 RWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGLK 361
W L L GSS F E+ +G+ + ++P++ + M T + M A+
Sbjct: 127 PWHFLGSWLQGSSLTAF-EAAHGMDLWNYGNQNPEFFSLIGEIMATDSRMMSLAI----- 180
Query: 362 RGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
E + ++SLVDVGG G +A+ I AF
Sbjct: 181 -------RECKEIFEGLSSLVDVGGGTGTMARGICEAF 211
>ref|XP_002525159.1| o-methyltransferase, putative [Ricinus communis]
gi|223535618|gb|EEF37286.1| o-methyltransferase,
putative [Ricinus communis]
Length = 356
Score = 73.2 bits (178), Expect(2) = 2e-20
Identities = 48/158 (30%), Positives = 75/158 (47%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEFA 181
++MR ++H F K + ++ + Y LT SK+LLKD+ S+ P L+ D + E
Sbjct: 71 RLMRILIHSGFFAK-AKLKENDEAEGYVLTKASKLLLKDNPFSVTPFLLAMLDRILTE-- 127
Query: 182 RWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGLK 361
W +L + F +H G+ F+E +PK N M + ++ L+D
Sbjct: 128 PWHYLSTWFQNDDATAFTTAH-GMGFWEYAGNEPKLNNFFNEAMASDARLVMRVLID--- 183
Query: 362 RGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
E++G + SLVDVGG G +AKAI AF
Sbjct: 184 --------EYKGVFEGLKSLVDVGGGTGTMAKAIAKAF 213
Score = 52.0 bits (123), Expect(2) = 2e-20
Identities = 23/50 (46%), Positives = 30/50 (60%)
Frame = +1
Query: 466 KCVPHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILH 615
K P L C V DLPHV+ + L +VA DMF+ +P D +LLK I+H
Sbjct: 211 KAFPRLDCIVFDLPHVVAGLAGSENLKYVAGDMFEGVPPADAILLKWIMH 260
>emb|CAN75970.1| hypothetical protein VITISV_009881 [Vitis vinifera]
Length = 354
Score = 70.5 bits (171), Expect(2) = 2e-20
Identities = 29/48 (60%), Positives = 37/48 (77%)
Frame = +1
Query: 475 PHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
PHLKCTV+DLP V+ N+PK LD+V DMF SIPS D + +K++LHN
Sbjct: 212 PHLKCTVLDLPQVVANLPKSENLDYVGGDMFQSIPSADAIFIKSVLHN 259
Score = 54.7 bits (130), Expect(2) = 2e-20
Identities = 47/158 (29%), Positives = 73/158 (46%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEFA 181
Q+MR +VH F + +QE Y LTP S++L+K + SLAP+++ D +
Sbjct: 72 QLMRLLVHSGFFAMQKVDENQEG---YVLTPPSRLLVKGNATSLAPIVLGMLDPVL--VT 126
Query: 182 RWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGLK 361
W L L GSS F E+ +G+ + ++P+ + M T + M A+
Sbjct: 127 PWHFLGSWLQGSSLTAF-EAAHGMDLWNYGNQNPEXFSXIGEIMATDSRMMSLAI----- 180
Query: 362 RGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
E + +SLVDVGG G +A+ I AF
Sbjct: 181 -------RECKEIFEGXSSLVDVGGGTGTMARGICEAF 211
>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase [Coptis chinensis]
Length = 350
Score = 67.0 bits (162), Expect(2) = 3e-20
Identities = 51/159 (32%), Positives = 80/159 (50%), Gaps = 1/159 (0%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEFA 181
+I+RY+V M + V K+ D E K Y L P++ +L ++ KRS+ P+++ Q +F
Sbjct: 71 RILRYLVKMEILRV-EKSDDGEKK--YALEPIATLLSRNAKRSMVPMILGMT---QKDFM 124
Query: 182 R-WAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGL 358
W + +GL S + T +E G+ +E +E P +Q GM T + +L+ G
Sbjct: 125 TPWHSMKDGL--SDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGS 182
Query: 359 KRGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
+ D +G + SLVDVGG G KAI AF
Sbjct: 183 R-------DMFQG----IDSLVDVGGGNGTTVKAISDAF 210
Score = 57.8 bits (138), Expect(2) = 3e-20
Identities = 25/48 (52%), Positives = 33/48 (68%)
Frame = +1
Query: 475 PHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
PH+KCT+ DLPHVI N P ++ + DMF S+PS +LLK ILH+
Sbjct: 211 PHIKCTLFDLPHVIANSYDHPNIERIGGDMFKSVPSAQAILLKLILHD 258
>ref|XP_003633246.1| PREDICTED: tabersonine 16-O-methyltransferase isoform 2 [Vitis
vinifera]
Length = 351
Score = 68.9 bits (167), Expect(2) = 4e-20
Identities = 49/159 (30%), Positives = 73/159 (45%), Gaps = 1/159 (0%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQ-EDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEF 178
++MR +VH F + AD E + Y LT S+ LLKD S APLL++Q D
Sbjct: 72 RLMRVLVHSGFFA--QQQADHNEQEQLYSLTYASRFLLKDEPTSGAPLLLVQVDP---HL 126
Query: 179 ARWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGL 358
H + +S T + + YG PF++ +PK+N M + + + +V
Sbjct: 127 TNPCHFLSDWFRNSDPTPFVTAYGKPFWDYAAHEPKFNNFFNEAMASDSQLIASVVVGEC 186
Query: 359 KRGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
K R ++SL+DVGG G +AK I AF
Sbjct: 187 KE-----------VFRGLSSLIDVGGGTGTMAKVIAKAF 214
Score = 55.5 bits (132), Expect(2) = 4e-20
Identities = 24/46 (52%), Positives = 31/46 (67%)
Frame = +1
Query: 466 KCVPHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLK 603
K PHLKCTV D PHV+ N+ L+FV D+F++IP D +LLK
Sbjct: 212 KAFPHLKCTVFDQPHVVANLQGGENLEFVGGDIFEAIPPADAILLK 257
>ref|XP_006364329.1| PREDICTED: uncharacterized protein LOC102587683 [Solanum tuberosum]
Length = 829
Score = 65.1 bits (157), Expect(2) = 7e-20
Identities = 28/48 (58%), Positives = 35/48 (72%)
Frame = +1
Query: 475 PHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
PHLKCTV+DLPHV+ N+ L +V DMF SIPS D +L K++LHN
Sbjct: 685 PHLKCTVLDLPHVVANMSDTHNLKYVGGDMFHSIPSADAILFKHVLHN 732
Score = 58.5 bits (140), Expect(2) = 7e-20
Identities = 46/160 (28%), Positives = 75/160 (46%), Gaps = 2/160 (1%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEFA 181
++MR +V+ F E++ Y LTP SK+LLK +L+P + D +
Sbjct: 541 RLMRLLVYSGFFATTNTILLDENQQGYILTPSSKLLLKSEIPNLSPYVRAIVDPV--SVN 598
Query: 182 RWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTIT--MCMVDALVDG 355
W L + + T +E+ +G P +E ++P++N M + + MC+V
Sbjct: 599 PWQSLGDWFLLGNETTPFETAHGAPMWEFFHQNPRFNNVFNEAMASDSQMMCLV------ 652
Query: 356 LKRGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
+K K + D + SLVDVGG G +A IL+AF
Sbjct: 653 VKDCKEVFQD--------MDSLVDVGGGTGIIANTILAAF 684
>ref|XP_006364318.1| PREDICTED: trans-resveratrol di-O-methyltransferase-like [Solanum
tuberosum]
Length = 357
Score = 67.8 bits (164), Expect(2) = 7e-20
Identities = 28/48 (58%), Positives = 36/48 (75%)
Frame = +1
Query: 475 PHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
PHLKCTV+DLPHV+ N+P L +V DMF SIPS D +L K+++HN
Sbjct: 213 PHLKCTVLDLPHVVANMPDTENLKYVGGDMFHSIPSADAILFKHVMHN 260
Score = 55.8 bits (133), Expect(2) = 7e-20
Identities = 46/161 (28%), Positives = 79/161 (49%), Gaps = 3/161 (1%)
Frame = +2
Query: 2 QIMRYMVHMNLF-TVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEF 178
++MR +V+ F T + E++ Y LTP SK+LL+ +L+P D +
Sbjct: 69 RLMRLLVYSGFFATREFLDENSENQQGYVLTPSSKLLLRSEIPNLSPFARAMVDPVMVN- 127
Query: 179 ARWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTIT--MCMVDALVD 352
W L + + G+ + F E+ +G P ++ +++P++N M + + MC+V
Sbjct: 128 -PWQSLGDWILGNETTPF-ETAHGAPMWQFCDQNPRFNNVFNEAMASDSQMMCLV----- 180
Query: 353 GLKRGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
+K K + D + SLVDVGG G +A IL+AF
Sbjct: 181 -VKDCKQVFQD--------MDSLVDVGGGTGIIANTILAAF 212
>ref|XP_002305104.2| 6a-hydroxymaackiain methyltransferase family protein [Populus
trichocarpa] gi|550340357|gb|EEE85615.2|
6a-hydroxymaackiain methyltransferase family protein
[Populus trichocarpa]
Length = 357
Score = 62.8 bits (151), Expect(2) = 1e-19
Identities = 26/48 (54%), Positives = 36/48 (75%)
Frame = +1
Query: 475 PHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
PH+KCTV+DLP V+ N+P+ L FV DMF SIPS D +++K++L N
Sbjct: 215 PHMKCTVLDLPQVVANLPESENLKFVGGDMFKSIPSADAIIIKSVLLN 262
Score = 59.7 bits (143), Expect(2) = 1e-19
Identities = 44/158 (27%), Positives = 80/158 (50%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEFA 181
++MR +VH +F +QE+++ Y LT S++LL D +L+P ++ D + +
Sbjct: 72 RLMRILVHSGVFDTTKMHENQEEEEGYVLTSSSRLLLNDSPTNLSPAVIAILDPVL--MS 129
Query: 182 RWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGLK 361
W L E + G +T +E+ +G+ F E +++ K+ + M + + +V +V
Sbjct: 130 PWFSLGEWIQG-KERTPFEACHGMSFSEYGKRNIKFINNLNEAMASDSQ-LVSLVV---- 183
Query: 362 RGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
EH +V SLVDVGG G +A++I A+
Sbjct: 184 -------KEHMEIFESVDSLVDVGGGTGTLARSIADAY 214
>ref|XP_002281368.2| PREDICTED: 8-hydroxyquercetin 8-O-methyltransferase-like [Vitis
vinifera]
Length = 357
Score = 64.3 bits (155), Expect(2) = 2e-19
Identities = 47/158 (29%), Positives = 73/158 (46%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEFA 181
++MR +VH F + E+++ Y LT S++LL D S+ PL++ D + +
Sbjct: 72 RLMRILVHSG-FLAAQRVQQGEEEEGYVLTDASRLLLMDDSLSIRPLVLAMLDPILTK-- 128
Query: 182 RWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGLK 361
W H + + T + + Y PF++ +P+ N M + + L LK
Sbjct: 129 PW-HYLSAWFQNDDPTPFHTAYERPFWDYAGHEPQLNNSFNEAMASDARLLTSVL---LK 184
Query: 362 RGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
GK G + SLVDVGG G VAKAI +AF
Sbjct: 185 EGK--------GVFAGLNSLVDVGGGTGKVAKAIANAF 214
Score = 57.8 bits (138), Expect(2) = 2e-19
Identities = 25/48 (52%), Positives = 34/48 (70%)
Frame = +1
Query: 475 PHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
PHL CTV+DLPHV+ + L++ A DMF++IP D +LLK ILH+
Sbjct: 215 PHLNCTVLDLPHVVAGLQGSKNLNYFAGDMFEAIPPADAILLKWILHD 262
>ref|XP_002517827.1| o-methyltransferase, putative [Ricinus communis]
gi|223542809|gb|EEF44345.1| o-methyltransferase,
putative [Ricinus communis]
Length = 356
Score = 72.4 bits (176), Expect(2) = 2e-19
Identities = 47/158 (29%), Positives = 78/158 (49%)
Frame = +2
Query: 2 QIMRYMVHMNLFTVITKTADQEDKDYYGLTPVSKILLKDHKRSLAPLLMLQADDLQNEFA 181
++MR +VH F K ++++ + LT SK+LLKD+ S+ P L+ D + E
Sbjct: 71 RLMRILVHSGFFAN-AKIKGNDEEEGFVLTSASKLLLKDNPLSVTPFLLAMLDPILTE-- 127
Query: 182 RWAHLIEGLDGSSSKTFWESHYGLPFYEMVEKDPKWNQKMINGMTTITMCMVDALVDGLK 361
W H + + T +++ +G+PF+E +PK+N M + ++ L+D
Sbjct: 128 PW-HYVSTWFQNDDATPFDTVHGMPFWEYAGNEPKFNNFFNEAMASDARLVMRVLID--- 183
Query: 362 RGKVIDDDEHEGTARTVTSLVDVGGNKGNVAKAILSAF 475
E++G + SLVDVGG G +A AI AF
Sbjct: 184 --------EYKGVFEGLNSLVDVGGGTGTMAAAIAKAF 213
Score = 49.7 bits (117), Expect(2) = 2e-19
Identities = 21/51 (41%), Positives = 30/51 (58%)
Frame = +1
Query: 466 KCVPHLKCTVMDLPHVIQNVPKDPKLDFVAVDMFDSIPSVDFVLLKNILHN 618
K P L C V DLPHV+ + L +V +MF+ +P D +LLK I+H+
Sbjct: 211 KAFPQLDCIVFDLPHVVAGLQSSANLKYVGGNMFEGVPPADAILLKWIIHD 261