BLASTX nr result
ID: Cocculus23_contig00010567
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cocculus23_contig00010567
(754 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransf... 204 3e-50
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica] 132 2e-28
gb|AAP45313.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 126 7e-27
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra... 126 9e-27
gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 125 2e-26
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc... 124 4e-26
ref|XP_004232928.1| PREDICTED: trans-resveratrol di-O-methyltran... 124 4e-26
dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransfe... 124 4e-26
ref|XP_006364329.1| PREDICTED: uncharacterized protein LOC102587... 122 1e-25
gb|AAR09603.1| O-methyltransferase [Mentha x piperita] 122 2e-25
sp|Q6VMW0.1|Q8OMT_MENPI RecName: Full=8-hydroxyquercetin 8-O-met... 122 2e-25
ref|XP_006364328.1| PREDICTED: trans-resveratrol di-O-methyltran... 121 2e-25
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 120 4e-25
ref|XP_006364318.1| PREDICTED: trans-resveratrol di-O-methyltran... 120 5e-25
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr... 119 9e-25
gb|ACO90238.1| norcoclaurine 6-O-methyltransferase [Thalictrum f... 119 9e-25
emb|CAN75970.1| hypothetical protein VITISV_009881 [Vitis vinifera] 119 1e-24
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth... 119 1e-24
ref|XP_002278127.1| PREDICTED: isoflavone-7-O-methyltransferase ... 119 1e-24
ref|XP_006377431.1| 6a-hydroxymaackiain methyltransferase family... 117 3e-24
>sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransferase; Short=CoOMT;
AltName: Full=Tetrahydrocolumbamine
2-O-methyltransferase gi|24210466|dbj|BAC22084.1|
columbamine O-methyltransferase [Coptis japonica]
Length = 351
Score = 204 bits (519), Expect = 3e-50
Identities = 110/221 (49%), Positives = 150/221 (67%), Gaps = 1/221 (0%)
Frame = +2
Query: 95 TEEQEEEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHGPLTLSELASKLQIESIN 274
T + ++E+KA+ Q+ KHM+ FAETI LR V LGIPD IH +GP+TLS+L + L ++S +
Sbjct: 6 TFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGPVTLSQLVTHLPLKSTS 65
Query: 275 MDRFELTMRYMVHMNLLTAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDP 454
+DRF MRY+VHM L T D+ E KY LTPASKLL+ H + KSLA +VM+QT P
Sbjct: 66 IDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLV---HGHQKSLAPYVMLQTHP 122
Query: 455 EELFVSRHLVESLGGAKSCWELFYGVPVNEKLEKDEKWSQ-MSDGMNGYTLCMVEAIVDG 631
EE V H++ L G K WE + EK E D + ++ ++D M ++ M+ A+V G
Sbjct: 123 EEFSVWSHVINVLDGKKPYWE-SNDTSMYEKTEGDPEINEILNDAMTSHSTFMLPALVSG 181
Query: 632 LKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
L +E V+D V S+VDVGGN+GVVAK I+ AFPH+KC+VMD
Sbjct: 182 LMKENVLD-GVASIVDVGGNSGVVAKGIVDAFPHVKCSVMD 221
>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
Length = 351
Score = 132 bits (331), Expect = 2e-28
Identities = 77/216 (35%), Positives = 121/216 (56%), Gaps = 2/216 (0%)
Frame = +2
Query: 113 EMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMDRFE 289
+ + ++ KH+Y FAE++ L+ AV+L I D IH HG P+TLSELASKL ++ +++DR
Sbjct: 10 DQATQAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPVQPVDLDRLY 69
Query: 290 LTMRYMVHMNLLTAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDPEELFV 469
MRY+VHM L DE KY L P +K L+K KS+ S +++ D + +
Sbjct: 70 RVMRYLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGW---EKSMVSAILLINDKDFMSP 126
Query: 470 SRHLVESLGGAKSCWELFYGVPVNEKLEKD-EKWSQMSDGMNGYTLCMVEAIVDGLKREK 646
HL E L + ++ G + + + + +K ++GM + + A+V+
Sbjct: 127 WHHLKEGLVNNSNAFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALVNECGN-- 184
Query: 647 VIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
I Q + +LVDVGG TG KA+ AFPH+KC++ D
Sbjct: 185 -IFQGIQTLVDVGGGTGTAVKAVSKAFPHIKCSIYD 219
>gb|AAP45313.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 1 [Papaver somniferum]
Length = 354
Score = 126 bits (317), Expect = 7e-27
Identities = 81/228 (35%), Positives = 126/228 (55%), Gaps = 3/228 (1%)
Frame = +2
Query: 80 GNLAMTEEQEEEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQI-HKHGPLTLSELASKL 256
G+ E E ++K + QL +Y +A+++ LRC V++GI D I + +G +TLSEL SKL
Sbjct: 2 GSSIDAETHEVDIKDQAQLWNIIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKL 61
Query: 257 QIESINMDRFELTMRYMVHMNLLTAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFV 436
+ ++N D +RY+VH+N+L A + Y L P LLLK S +S+A +
Sbjct: 62 PLSNVNSDNLYRLLRYLVHLNILGQQTCAAGVDRVYSLKPVGTLLLKDS---ERSMAPVI 118
Query: 437 MVQTDPEELFVSRHLVESLG-GAKSCWELFYGVPVNEKLEKDEKWSQMSD-GMNGYTLCM 610
+ + + LFV + E LG G+ + +E G+ + + LE + SQ+ D G G T +
Sbjct: 119 LGLSQKDFLFVWNFVKEGLGTGSTTAFEKAMGMDMWKYLEVNPNQSQLFDEGQAGETRLL 178
Query: 611 VEAIVDGLKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
+ ++ + Q + SLVDVGG G KAI AFPH+KCT+ D
Sbjct: 179 TKTLLVDCRD---TFQGMDSLVDVGGGNGTTIKAIHEAFPHIKCTLYD 223
>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase
[Thalictrum flavum subsp. glaucum]
Length = 348
Score = 126 bits (316), Expect = 9e-27
Identities = 80/217 (36%), Positives = 125/217 (57%), Gaps = 3/217 (1%)
Frame = +2
Query: 113 EMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMDRFE 289
++KA+ + K +Y FA+++ LRCAV+LGI D I + P+ L++LASKL I ++N+D
Sbjct: 12 DIKAQAHVWKIIYGFADSLVLRCAVELGIFDIIDNNNQPIPLADLASKLPISNVNLDNLF 71
Query: 290 LTMRYMVHMNLLTAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDPEELFV 469
+RY+V M LL+ AD+ KY L P +KL+L+ N +S+ V+ T + +
Sbjct: 72 RILRYLVKMELLSYAADD-----KYALEPIAKLVLR---NEKRSMVPMVLGMTQKDFMTP 123
Query: 470 SRHLVESL-GGAKSCWELFYGVPVNEKLEKDEKWSQM-SDGMNGYTLCMVEAIVDGLKRE 643
+ + L + +E G+ + E LE SQ+ ++GM G T + +++ G
Sbjct: 124 WHSMKDGLTDNGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISG---S 180
Query: 644 KVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
K + QS+ SLVDVGG G KAI AFPH+KCT+ D
Sbjct: 181 KDMFQSIDSLVDVGGGNGTTIKAISVAFPHIKCTLFD 217
>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase [Coptis chinensis]
Length = 350
Score = 125 bits (314), Expect = 2e-26
Identities = 74/216 (34%), Positives = 121/216 (56%), Gaps = 2/216 (0%)
Frame = +2
Query: 113 EMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMDRFE 289
++KA+ + K +Y FA+++ LRCAV+LGI D + + P+ L++LASKL + ++N D
Sbjct: 11 DIKAQAHVWKIIYGFADSLVLRCAVELGIVDIVDNNNQPMALADLASKLPVSNVNCDNLY 70
Query: 290 LTMRYMVHMNLLTAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDPEELFV 469
+RY+V M +L + +GE KY L P + LL S N +S+ ++ T + +
Sbjct: 71 RILRYLVKMEILR-VEKSDDGEKKYALEPIATLL---SRNAKRSMVPMILGMTQKDFMTP 126
Query: 470 SRHLVESLGGAKSCWELFYGVPVNEKLEKDEKWSQM-SDGMNGYTLCMVEAIVDGLKREK 646
+ + L + +E G+ + E LE SQ+ ++GM G T + +++ G +
Sbjct: 127 WHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISG---SR 183
Query: 647 VIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
+ Q + SLVDVGG G KAI AFPH+KCT+ D
Sbjct: 184 DMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFD 219
>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
gi|9711916|dbj|BAB08005.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
4'-O-methyltransferase [Coptis japonica]
Length = 350
Score = 124 bits (311), Expect = 4e-26
Identities = 74/216 (34%), Positives = 120/216 (55%), Gaps = 2/216 (0%)
Frame = +2
Query: 113 EMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMDRFE 289
++KA+ + K +Y FA+++ LRCAV+LGI D I + P+ L++LASKL + +N D
Sbjct: 11 DIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDNLY 70
Query: 290 LTMRYMVHMNLLTAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDPEELFV 469
+RY+V M +L + +G+ KY L P + LL S N +S+ ++ T + +
Sbjct: 71 RILRYLVKMEILR-VEKSDDGQKKYALEPIATLL---SRNAKRSMVPMILGMTQKDFMTP 126
Query: 470 SRHLVESLGGAKSCWELFYGVPVNEKLEKDEKWSQM-SDGMNGYTLCMVEAIVDGLKREK 646
+ + L + +E G+ + E LE SQ+ ++GM G T + +++ G +
Sbjct: 127 WHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISG---SR 183
Query: 647 VIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
+ Q + SLVDVGG G KAI AFPH+KCT+ D
Sbjct: 184 DMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFD 219
>ref|XP_004232928.1| PREDICTED: trans-resveratrol di-O-methyltransferase-like [Solanum
lycopersicum]
Length = 357
Score = 124 bits (311), Expect = 4e-26
Identities = 84/224 (37%), Positives = 127/224 (56%), Gaps = 9/224 (4%)
Frame = +2
Query: 110 EEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMDRF 286
E +A+ + KH ++FA ++ L CA+QLGIPD IH H P+TLS+L S L++ D
Sbjct: 8 EMFEAQAHIYKHAFSFANSMVLGCAIQLGIPDVIHSHKQPMTLSQLVSHLKLPLEKSDAI 67
Query: 287 ELTMRYMVHMNLL--TAIADEANGETKYG--LTPASKLLLKSSHNNNKSLASFVMVQTDP 454
MR +V+ T DE N E++ G LTP+SKLLLKS N L+ F DP
Sbjct: 68 HRLMRLLVYSGFFATTDFLDE-NSESQQGYVLTPSSKLLLKSEIPN---LSPFARAMIDP 123
Query: 455 EELFVSRHLVE-SLGGAKSCWELFYGVPVNEKLEKDEKWSQMSD---GMNGYTLCMVEAI 622
+ + L + LG + +E +G P+ + +++ ++S + + + +C+V
Sbjct: 124 VMVNPWQSLGDWFLGNETTPFETAHGAPMWKFCDQNPRFSNVFNEAMASDSQMMCLV--- 180
Query: 623 VDGLKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
+K K + Q + SLVDVGG TG++A IL+AFPHLKCTV+D
Sbjct: 181 ---VKDCKQVFQEIDSLVDVGGGTGIIANTILAAFPHLKCTVLD 221
>dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase [Eschscholzia
californica]
Length = 353
Score = 124 bits (311), Expect = 4e-26
Identities = 77/222 (34%), Positives = 121/222 (54%), Gaps = 5/222 (2%)
Frame = +2
Query: 104 QEEEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQI-HKHGPLTLSELASKLQIESINMD 280
+E ++KA+ L +Y FA+++ LR AV+LGI D I + +G +T+SELASKL I ++N D
Sbjct: 7 EEVDIKAQAHLWNIIYGFADSLVLRSAVELGIADIIKNNNGSITVSELASKLPISNVNSD 66
Query: 281 RFELTMRYMVHMNLLTAIADEANG---ETKYGLTPASKLLLKSSHNNNKSLASFVMVQTD 451
+RY+VHM +L NG + Y L P LL+K + N + V+ T
Sbjct: 67 NLYRVLRYLVHMGILKETKSTINGGEIKKLYSLEPVGSLLVKDAERN---MVPIVLGMTQ 123
Query: 452 PEELFVSRHLVESLGGAKSCWELFYGVPVNEKLE-KDEKWSQMSDGMNGYTLCMVEAIVD 628
+ + ++ E LG + +E G+ + E LE E+ + GM G T + + +++
Sbjct: 124 QDFMIPWHYIKEGLGEGSTAFEKGMGMTLWEYLEGHPEQGHLFNVGMEGETRLLTKTLIE 183
Query: 629 GLKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
K + ++SLVDVGG G KAI AFPH+KC++ D
Sbjct: 184 SCKD---TFEGLSSLVDVGGGNGTTIKAISEAFPHIKCSLYD 222
>ref|XP_006364329.1| PREDICTED: uncharacterized protein LOC102587683 [Solanum tuberosum]
Length = 829
Score = 122 bits (307), Expect = 1e-25
Identities = 81/228 (35%), Positives = 127/228 (55%), Gaps = 10/228 (4%)
Frame = +2
Query: 101 EQEEEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINM 277
++E + + + KH ++FA ++ L CA+QLGIPD IH H P+TLS+L S+L++
Sbjct: 477 DKEWMFQGQAHIYKHAFSFANSMVLGCAIQLGIPDVIHSHKQPMTLSQLVSELKLPPAKS 536
Query: 278 DRFELTMRYMVHMNLL----TAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQ 445
D MR +V+ T + DE + Y LTP+SKLLLKS N L+ +V
Sbjct: 537 DAIHRLMRLLVYSGFFATTNTILLDE--NQQGYILTPSSKLLLKSEIPN---LSPYVRAI 591
Query: 446 TDPEELFVSRHLVE--SLGGAKSCWELFYGVPVNEKLEKDEKWSQMSD---GMNGYTLCM 610
DP + + L + LG + +E +G P+ E ++ +++ + + + +C+
Sbjct: 592 VDPVSVNPWQSLGDWFLLGNETTPFETAHGAPMWEFFHQNPRFNNVFNEAMASDSQMMCL 651
Query: 611 VEAIVDGLKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
V +K K + Q + SLVDVGG TG++A IL+AFPHLKCTV+D
Sbjct: 652 V------VKDCKEVFQDMDSLVDVGGGTGIIANTILAAFPHLKCTVLD 693
>gb|AAR09603.1| O-methyltransferase [Mentha x piperita]
Length = 361
Score = 122 bits (305), Expect = 2e-25
Identities = 80/219 (36%), Positives = 119/219 (54%), Gaps = 3/219 (1%)
Frame = +2
Query: 107 EEEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMDR 283
EE + A+ + H++++ +++L+ A+QLGIPD IHKHG P+TLS+LA L I D
Sbjct: 12 EELLDAEAHVWNHIFSYINSMSLKSALQLGIPDIIHKHGNPITLSQLADALNINKAKTDG 71
Query: 284 FELTMRYMVHMNLLTAIADEANGETK-YGLTPASKLLLKSSHNNNKSLASFVMVQTDPEE 460
MR + H + E GE + Y LT AS+LLL+ + SLA ++ DP
Sbjct: 72 LFRLMRLLEHSKFFDKVKVEGEGEEEAYSLTRASRLLLR---DEPSSLAPYIRAMLDPNF 128
Query: 461 LFVSRHLVESLGG-AKSCWELFYGVPVNEKLEKDEKWSQMSDGMNGYTLCMVEAIVDGLK 637
+ HL E LG S +E +G + E +E+W+Q+ + +V +I+ +K
Sbjct: 129 MDPFHHLSEWLGSECPSPFEFKHGRSLWEYAGIEERWNQLFNQAMANDAKLVTSIL--VK 186
Query: 638 REKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
+ I Q + SLVDVGG G VAK + AFP LK V+D
Sbjct: 187 ECRHIFQGLESLVDVGGGAGTVAKVVADAFPGLKAVVLD 225
>sp|Q6VMW0.1|Q8OMT_MENPI RecName: Full=8-hydroxyquercetin 8-O-methyltransferase; AltName:
Full=Flavonol 8-O-methyltransferase
gi|38047395|gb|AAR09600.1| flavonoid
8-O-methyltransferase [Mentha x piperita]
Length = 366
Score = 122 bits (305), Expect = 2e-25
Identities = 79/227 (34%), Positives = 119/227 (52%), Gaps = 10/227 (4%)
Frame = +2
Query: 104 QEEEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMD 280
++E ++A+ + H+Y++ +++L+CA+QLGIPD IHKHG P+TLS+LA L I
Sbjct: 10 KQELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHGNPITLSQLADALNINKAKSH 69
Query: 281 RFELTMRYMVHMNLLTAI--------ADEANGETKYGLTPASKLLLKSSHNNNKSLASFV 436
MR +VH + DE E Y LTPAS+LLL+S S+A F
Sbjct: 70 GLFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRS---EPLSVAPFA 126
Query: 437 MVQTDPEELFVSRHLVESL-GGAKSCWELFYGVPVNEKLEKDEKWSQMSDGMNGYTLCMV 613
+ +DP HL E A + ++ YG+ E D++ + + + V
Sbjct: 127 LAMSDPVYTETWHHLSEWFRNDAVAAFDTKYGMTFPEYAVADDRLNVLFNEAMACDAGFV 186
Query: 614 EAIVDGLKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
+I+ RE I + S+VDVGG TG AK I +AFP ++CTV+D
Sbjct: 187 NSILTTECRE--IFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLD 231
>ref|XP_006364328.1| PREDICTED: trans-resveratrol di-O-methyltransferase-like [Solanum
tuberosum]
Length = 354
Score = 121 bits (304), Expect = 2e-25
Identities = 80/223 (35%), Positives = 124/223 (55%), Gaps = 8/223 (3%)
Frame = +2
Query: 110 EEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMDRF 286
E + + + KH ++A + L CA+QLGIPD IH H P+TLS+L S+L++ D
Sbjct: 4 EMFQGQAHIYKHALSYANCMVLSCAIQLGIPDVIHSHKQPMTLSQLVSQLKLPPQKSDAV 63
Query: 287 ELTMRYMVHMNLLTA---IADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDPE 457
MR +V+ + + + + Y LTP+SKLLLKS N L++FV +P
Sbjct: 64 YRVMRLLVYSGFFATRDFLDENSENQQGYILTPSSKLLLKSEIPN---LSTFVRAMVEPA 120
Query: 458 ELFVSRHLVE-SLGGAKSCWELFYGVPVNEKLEKDEKWSQMSD---GMNGYTLCMVEAIV 625
+ + L + LG + +E G P+ E +++ +++ + + + +C+V
Sbjct: 121 MVNPWQSLGDWFLGDKTTPFETANGAPIWEFGDQNPRFNNVFNEAMASDSQMMCLVA--- 177
Query: 626 DGLKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
K K + Q + SLVDVGG TGV+AKAIL+AFPHLKCTV+D
Sbjct: 178 ---KDCKQVFQDMNSLVDVGGGTGVIAKAILAAFPHLKCTVLD 217
>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 2 [Papaver somniferum]
gi|571330882|gb|AHF27397.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'- O-methyltransferase 2 [synthetic construct]
Length = 357
Score = 120 bits (302), Expect = 4e-25
Identities = 73/220 (33%), Positives = 123/220 (55%), Gaps = 3/220 (1%)
Frame = +2
Query: 104 QEEEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKH-GPLTLSELASKLQIESINMD 280
QE +K + QL +Y FA+++ LRCAV++GI D I + G +TL++LA+KL I +++ D
Sbjct: 13 QEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSD 72
Query: 281 RFELTMRYMVHMNLLTAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDPEE 460
+RY+VH+N++ E Y L P LLL+ + +S+ ++ T +
Sbjct: 73 YLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAE---RSMVPMILGMTQKDF 129
Query: 461 LFVSRHLVESLG-GAKSCWELFYGVPVNEKLEKDEKWSQM-SDGMNGYTLCMVEAIVDGL 634
+ + E LG G+ + +E G+ + + LE + SQ+ ++GM G T + + +++
Sbjct: 130 MVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDC 189
Query: 635 KREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
+ Q + SLVD+GG G KAI AFPH+KCT+ D
Sbjct: 190 RDTF---QGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYD 226
>ref|XP_006364318.1| PREDICTED: trans-resveratrol di-O-methyltransferase-like [Solanum
tuberosum]
Length = 357
Score = 120 bits (301), Expect = 5e-25
Identities = 76/223 (34%), Positives = 124/223 (55%), Gaps = 8/223 (3%)
Frame = +2
Query: 110 EEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMDRF 286
E + + + KH ++FA ++ L CA+QLGIPD IH H P+TLS+L S+L++ D
Sbjct: 8 EMFQGQAHIYKHAFSFANSMVLGCAIQLGIPDVIHSHKQPMTLSQLVSELKLLPEKSDAI 67
Query: 287 ELTMRYMVHMNLLTA---IADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDPE 457
MR +V+ + + + + Y LTP+SKLLL+S N L+ F DP
Sbjct: 68 HRLMRLLVYSGFFATREFLDENSENQQGYVLTPSSKLLLRSEIPN---LSPFARAMVDPV 124
Query: 458 ELFVSRHLVE-SLGGAKSCWELFYGVPVNEKLEKDEKWSQMSD---GMNGYTLCMVEAIV 625
+ + L + LG + +E +G P+ + +++ +++ + + + +C+V
Sbjct: 125 MVNPWQSLGDWILGNETTPFETAHGAPMWQFCDQNPRFNNVFNEAMASDSQMMCLV---- 180
Query: 626 DGLKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
+K K + Q + SLVDVGG TG++A IL+AFPHLKCTV+D
Sbjct: 181 --VKDCKQVFQDMDSLVDVGGGTGIIANTILAAFPHLKCTVLD 221
>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
glaucum]
Length = 350
Score = 119 bits (299), Expect = 9e-25
Identities = 74/221 (33%), Positives = 120/221 (54%), Gaps = 5/221 (2%)
Frame = +2
Query: 107 EEEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMDR 283
+E + ++ +L +Y FA+++ L+ AVQL + + IH HG P+TLSEL+ L + +N D
Sbjct: 6 KENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQDA 65
Query: 284 FELTMRYMVHMNLLTAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDPEEL 463
+RY+VHM L T +GE +YGL P +K L+K +K + ++ TD + +
Sbjct: 66 LYRVLRYLVHMKLFTK--SSIDGELRYGLAPPAKFLVKGW---DKCMLGAILTITDKDFM 120
Query: 464 FVSRHLVESL---GGAKSCWELFYGVPVNEKL-EKDEKWSQMSDGMNGYTLCMVEAIVDG 631
+L E + G + +E G + + + E EK ++GM T ++ A+V
Sbjct: 121 APWHYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALV-- 178
Query: 632 LKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
K + +T++VDVGG TG + I AFPH+KCTV D
Sbjct: 179 -KECSSMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYD 218
>gb|ACO90238.1| norcoclaurine 6-O-methyltransferase [Thalictrum flavum]
Length = 225
Score = 119 bits (299), Expect = 9e-25
Identities = 74/221 (33%), Positives = 120/221 (54%), Gaps = 5/221 (2%)
Frame = +2
Query: 107 EEEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMDR 283
+E + ++ +L +Y FA+++ L+ AVQL + + IH HG P+TLSEL+ L + +N D
Sbjct: 6 KENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQDA 65
Query: 284 FELTMRYMVHMNLLTAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDPEEL 463
+RY+VHM L T +GE +YGL P +K L+K +K + ++ TD + +
Sbjct: 66 LYRVLRYLVHMKLFTK--SSIDGELRYGLAPPAKFLVKGW---DKCMLGAILTITDKDFM 120
Query: 464 FVSRHLVESL---GGAKSCWELFYGVPVNEKL-EKDEKWSQMSDGMNGYTLCMVEAIVDG 631
+L E + G + +E G + + + E EK ++GM T ++ A+V
Sbjct: 121 APWHYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALV-- 178
Query: 632 LKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
K + +T++VDVGG TG + I AFPH+KCTV D
Sbjct: 179 -KECSSMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYD 218
>emb|CAN75970.1| hypothetical protein VITISV_009881 [Vitis vinifera]
Length = 354
Score = 119 bits (298), Expect = 1e-24
Identities = 84/221 (38%), Positives = 122/221 (55%), Gaps = 4/221 (1%)
Frame = +2
Query: 104 QEEEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMD 280
+ E +A+ + KH+++F ++++L+CAVQLGIPD IH H P+TL ELAS +Q+
Sbjct: 9 RSELFEAQSFIYKHVFSFMDSMSLKCAVQLGIPDAIHNHNQPITLPELASAIQVPPEKTS 68
Query: 281 RFELTMRYMVHMNLLTAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDPEE 460
R MR +VH + N E Y LTP S+LL+K N SLA V+ DP
Sbjct: 69 RLHQLMRLLVHSGFFAMQKVDENQEG-YVLTPPSRLLVKG---NATSLAPIVLGMLDPV- 123
Query: 461 LFVSRHLVESL--GGAKSCWELFYGVPV-NEKLEKDEKWSQMSDGMNGYTLCMVEAIVDG 631
L H + S G + + +E +G+ + N + E +S + + M + M AI
Sbjct: 124 LVTPWHFLGSWLQGSSLTAFEAAHGMDLWNYGNQNPEXFSXIGEIMATDSRMMSLAI--- 180
Query: 632 LKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
+ K I + +SLVDVGG TG +A+ I AFPHLKCTV+D
Sbjct: 181 -RECKEIFEGXSSLVDVGGGTGTMARGICEAFPHLKCTVLD 220
>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase; Short=6-OMT
gi|9711914|dbj|BAB08004.1|
S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase [Coptis japonica]
Length = 347
Score = 119 bits (297), Expect = 1e-24
Identities = 76/222 (34%), Positives = 122/222 (54%), Gaps = 3/222 (1%)
Frame = +2
Query: 98 EEQEEEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHGP-LTLSELASKLQIESIN 274
E +++ + ++ +L +Y FAE++ L+CAVQL + + IH G +TLSEL+S+L + +N
Sbjct: 2 EVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVN 61
Query: 275 MDRFELTMRYMVHMNLLTAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDP 454
D MRY+VHM L T + +GE +YGL P +K L+K +K + ++ TD
Sbjct: 62 EDALYRVMRYLVHMKLFTKAS--IDGELRYGLAPPAKYLVKGW---DKCMVGSILAITDK 116
Query: 455 EELFVSRHLVESLGGAK-SCWELFYGVPV-NEKLEKDEKWSQMSDGMNGYTLCMVEAIVD 628
+ + +L + L G + +E G + E EK ++ M + ++ A+V
Sbjct: 117 DFMAPWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALV- 175
Query: 629 GLKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
K I +T+LVDVGG TG + I +AFPH+KCTV D
Sbjct: 176 --KECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYD 215
>ref|XP_002278127.1| PREDICTED: isoflavone-7-O-methyltransferase 9 [Vitis vinifera]
Length = 354
Score = 119 bits (297), Expect = 1e-24
Identities = 84/221 (38%), Positives = 123/221 (55%), Gaps = 4/221 (1%)
Frame = +2
Query: 104 QEEEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMD 280
+ E +A+ + KH+++F ++++L+CAVQLGIPD IH H P+TL ELAS +Q+
Sbjct: 9 RSELFEAQSFIYKHVFSFMDSMSLKCAVQLGIPDAIHNHNQPITLPELASAIQVPPEKTS 68
Query: 281 RFELTMRYMVHMNLLTAIADEANGETKYGLTPASKLLLKSSHNNNKSLASFVMVQTDPEE 460
R MR +VH + N E Y LTP S+LL+K N SLA V+ DP
Sbjct: 69 RLHQLMRLLVHSGFFAMQKVDENQEG-YVLTPPSRLLVKG---NATSLAPIVLGMLDPV- 123
Query: 461 LFVSRHLVESL--GGAKSCWELFYGVPV-NEKLEKDEKWSQMSDGMNGYTLCMVEAIVDG 631
L H + S G + + +E +G+ + N + E +S + + M + M AI
Sbjct: 124 LVTPWHFLGSWLQGSSLTAFEAAHGMDLWNYGNQNPEFFSLIGEIMATDSRMMSLAI--- 180
Query: 632 LKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
+ K I + ++SLVDVGG TG +A+ I AFPHLKCTV+D
Sbjct: 181 -RECKEIFEGLSSLVDVGGGTGTMARGICEAFPHLKCTVLD 220
>ref|XP_006377431.1| 6a-hydroxymaackiain methyltransferase family protein [Populus
trichocarpa] gi|550327721|gb|ERP55228.1|
6a-hydroxymaackiain methyltransferase family protein
[Populus trichocarpa]
Length = 358
Score = 117 bits (294), Expect = 3e-24
Identities = 83/221 (37%), Positives = 124/221 (56%), Gaps = 6/221 (2%)
Frame = +2
Query: 110 EEMKAKVQLGKHMYAFAETITLRCAVQLGIPDQIHKHG-PLTLSELASKLQIESINMDRF 286
E KA+ L KH+Y F E+++L+CAVQL IPD I K+ P+TL +L S L +
Sbjct: 11 ELFKAQCHLYKHIYYFIESMSLKCAVQLSIPDVIQKNNQPITLQQLVSALNFPESKANFL 70
Query: 287 ELTMRYMVHMNLLTAIADEANGETK---YGLTPASKLLLKSSHNNNKSLASFVMVQTDPE 457
+ MR +VH I N E Y LT +SKLLLK S SL+ V+ DP
Sbjct: 71 QRLMRVLVHSGFFDTIKIHDNQEEVEEGYVLTSSSKLLLKDSPT---SLSPVVLTMLDPV 127
Query: 458 ELFVSRHLVESLGGA-KSCWELFYGVPVNEKLEKDEKW-SQMSDGMNGYTLCMVEAIVDG 631
+ L E + G ++ +E ++G+ E +++ K+ + +++GM + +V +V
Sbjct: 128 LMNPWFSLGEWIQGKERTPFETYHGMSFGEYGKRNFKFINNLNEGMTSDSK-LVSLVV-- 184
Query: 632 LKREKVIDQSVTSLVDVGGNTGVVAKAILSAFPHLKCTVMD 754
K K I +SV SLVDVGG TG +A++I A+PH+KCTV+D
Sbjct: 185 -KEHKEIFESVDSLVDVGGGTGTLARSIADAYPHMKCTVLD 224