BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cocculus23_contig00008571
         (724 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   258   1e-66
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   191   3e-46
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   187   4e-45
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   179   9e-43
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   174   2e-41
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P...   174   3e-41
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   171   2e-40
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   170   5e-40
gb|AFK73713.1| cytochrome P450 [Papaver somniferum]                   168   2e-39
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum...   166   7e-39
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN...   166   1e-38
ref|XP_004234364.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   160   3e-37
ref|XP_006358870.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   159   7e-37
ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   159   1e-36
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              159   1e-36
ref|XP_004234365.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   157   3e-36
ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   157   5e-36
ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   155   1e-35
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   155   1e-35
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]   155   1e-35

>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  258 bits (660), Expect = 1e-66
 Identities = 126/190 (66%), Positives = 155/190 (81%), Gaps = 2/190 (1%)
 Frame = +2

Query: 161 ITLSLLSFIPFVIVLYLLFKPKH-NLPPSPRPSWPLIGNIPAIL-GSKTPLHVTLTDLAQ 334
           + ++L S IP ++V  LL KPK+ NLPP P P WPLIGN+P +   ++ PLH+TL ++A+
Sbjct: 3   LQIALFSLIPVILVFILLLKPKYKNLPPGPHP-WPLIGNLPILFTNTEVPLHITLANMAR 61

Query: 335 VHGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIKEMNKHSLVWSNCT 514
            HGP+M++ LGT PTV+AST EAAME+LKTHDR  S RHIRMSFR+K   K+SLVWS+CT
Sbjct: 62  THGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCT 121

Query: 515 DTWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNII 694
           D WKLLRK+ RTEIFSP+MLQ Q  VRE KV EL++FLR +EG+VVKISQFVFGTLLNI+
Sbjct: 122 DYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNIL 181

Query: 695 GNVVFSKDVF 724
           GNVVFSKDVF
Sbjct: 182 GNVVFSKDVF 191


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
           isozyme 2; AltName: Full=Cytochrome P450 80B2
           gi|9971208|dbj|BAB12433.1|
           (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  191 bits (484), Expect = 3e-46
 Identities = 99/192 (51%), Positives = 140/192 (72%), Gaps = 2/192 (1%)
 Frame = +2

Query: 155 EVITLSLLSFIPFVIVLYLL--FKPKHNLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDL 328
           EV++++++SF   + + ++L   +PK NLPP PRPS P++GN+   LG K   H     L
Sbjct: 2   EVLSIAIVSFSFLLFLFFILRDSRPK-NLPPGPRPS-PIVGNLLQ-LGDKP--HAEFAKL 56

Query: 329 AQVHGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIKEMNKHSLVWSN 508
           AQ +G L  + LG+   VVAS+  AA E+LKTHD+ LSGR++  SFR+KE  ++S+VWS 
Sbjct: 57  AQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSE 116

Query: 509 CTDTWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLN 688
           C D WKLLRK+ RTE+F+P+M++ Q  +RE K  E+V+FLRG+EG+VVKI + VFGTL+N
Sbjct: 117 CNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVN 176

Query: 689 IIGNVVFSKDVF 724
           I GN++FSKDVF
Sbjct: 177 IFGNLIFSKDVF 188


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  187 bits (474), Expect = 4e-45
 Identities = 96/191 (50%), Positives = 136/191 (71%), Gaps = 1/191 (0%)
 Frame = +2

Query: 155 EVITLSLLSFIPFVIVLYLLFKPKH-NLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDLA 331
           EV++++++SF   + + ++L  P+  NLPP PRP  P++GN+   LG K         LA
Sbjct: 2   EVVSIAIVSFSFLLFLFFILRDPRQKNLPPGPRPL-PIVGNLLQ-LGDKP--RAEFAKLA 57

Query: 332 QVHGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIKEMNKHSLVWSNC 511
           Q +G L  + LG+   VVAS+  AA EVLKTHD+ LSGR++  SFR+KE  ++S+VWS C
Sbjct: 58  QKYGQLFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSEC 117

Query: 512 TDTWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNI 691
            D WKLLRK+ RTE+F+P+M++ Q  +RE K  E+V+FLR +EG+VVKI + VFGTL+NI
Sbjct: 118 NDNWKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNI 177

Query: 692 IGNVVFSKDVF 724
            GN++FSKDVF
Sbjct: 178 FGNLIFSKDVF 188


>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
           AltName: Full=Cytochrome P450 80B2
           gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
           3'-hydroxylase [Eschscholzia californica]
          Length = 488

 Score =  179 bits (454), Expect = 9e-43
 Identities = 92/192 (47%), Positives = 135/192 (70%), Gaps = 2/192 (1%)
 Frame = +2

Query: 155 EVITLSLLSFIPFVIVLYLLFKPK--HNLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDL 328
           EV+T++L++ I    +LYLLF      NLPP P+P WP++GN+   LG K   H    +L
Sbjct: 2   EVVTVALIAVI-ISSILYLLFGSSGHKNLPPGPKP-WPIVGNLLQ-LGEKP--HAQFAEL 56

Query: 329 AQVHGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIKEMNKHSLVWSN 508
           AQ +G +  + +GT   VVAST  AA E+LKTHDR LS R++  SFR+K   ++S+VWS+
Sbjct: 57  AQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSD 116

Query: 509 CTDTWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLN 688
           CT+TWK LRK+ RTE+F+ +M++ Q  VRE K  E+VE+L  ++G+ VKI + +FGTL+N
Sbjct: 117 CTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVN 176

Query: 689 IIGNVVFSKDVF 724
           I GN++FS+++F
Sbjct: 177 IFGNLIFSQNIF 188


>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
           AltName: Full=Cytochrome P450 80B1
           gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
           3'-hydroxylase [Eschscholzia californica]
          Length = 487

 Score =  174 bits (442), Expect = 2e-41
 Identities = 90/189 (47%), Positives = 131/189 (69%), Gaps = 4/189 (2%)
 Frame = +2

Query: 170 SLLSFIPFVI--VLYLLF--KPKHNLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDLAQV 337
           S ++ I  +I  +LYLLF      NLPP P+P WP++GN+   LG K   H    +LAQ 
Sbjct: 3   STVALIAVIISSILYLLFGGSGHKNLPPGPKP-WPIVGNLLQ-LGEKP--HAQFAELAQT 58

Query: 338 HGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIKEMNKHSLVWSNCTD 517
           +G +  + +GT   VVAST  AA E+LKTHDR LS R++  SFR+K   ++S+VWS+CT+
Sbjct: 59  YGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTE 118

Query: 518 TWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNIIG 697
           TWK LRK+ RTE+F+ +M++ Q  VRE K  E+VE+L  ++G+ VKI + +FGTL+NI G
Sbjct: 119 TWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFG 178

Query: 698 NVVFSKDVF 724
           N++FS+++F
Sbjct: 179 NLIFSQNIF 187


>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
          Length = 486

 Score =  174 bits (441), Expect = 3e-41
 Identities = 91/191 (47%), Positives = 133/191 (69%), Gaps = 3/191 (1%)
 Frame = +2

Query: 161 ITLSLLSFIPFVIVLYLLFK---PKHNLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDLA 331
           +T+SL++ +     LYL+F+   PK  LPP P+P WP++GN+   LG K   H    +LA
Sbjct: 3   VTVSLVAVV-ITTFLYLIFRDSSPK-GLPPGPKP-WPIVGNLLQ-LGEKP--HSQFAELA 56

Query: 332 QVHGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIKEMNKHSLVWSNC 511
           + +G L  + LG+   VVAST  AA E+LKTHDR LSGR++  SFR+KE  ++S+VWS C
Sbjct: 57  KTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSEC 116

Query: 512 TDTWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNI 691
            +TWK LRK+ R E+F+ +M++ Q  +RE+K  E+VEFL+  +G  VKI + VFGTL+NI
Sbjct: 117 NETWKKLRKVCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNI 176

Query: 692 IGNVVFSKDVF 724
            GN++FS+++F
Sbjct: 177 FGNLIFSQNIF 187


>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  171 bits (433), Expect = 2e-40
 Identities = 89/177 (50%), Positives = 125/177 (70%), Gaps = 3/177 (1%)
 Frame = +2

Query: 203 LYLLFK---PKHNLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDLAQVHGPLMVVTLGTA 373
           LYL+F+   PK  LPP P+P WP++GN+   LG K   H     LA+ +G L  + LG+ 
Sbjct: 11  LYLIFRDSSPK-GLPPGPKP-WPIVGNLLQ-LGEKP--HSQFAQLAETYGDLFSLKLGSE 65

Query: 374 PTVVASTREAAMEVLKTHDRALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLRKLARTE 553
             VVAST  AA E+LKTHDR LSGR++  SFR+KE  ++S+VWS C +TWK LRK+ RTE
Sbjct: 66  TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 125

Query: 554 IFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNIIGNVVFSKDVF 724
           +F+ +M++ Q  VRE+K  E+VE+L+   G  VKI++ VFGTL+NI GN++FS+++F
Sbjct: 126 LFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIF 182


>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  170 bits (430), Expect = 5e-40
 Identities = 88/177 (49%), Positives = 125/177 (70%), Gaps = 3/177 (1%)
 Frame = +2

Query: 203 LYLLFK---PKHNLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDLAQVHGPLMVVTLGTA 373
           LYL+F+   PK  LPP P+P WP++GN+   LG K   H     LA+ +G L  + LG+ 
Sbjct: 11  LYLIFRDSSPK-GLPPGPKP-WPIVGNLLQ-LGEKP--HSQFAQLAETYGDLFSLKLGSE 65

Query: 374 PTVVASTREAAMEVLKTHDRALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLRKLARTE 553
             VVAST  AA E+LKTHDR LSGR++  SFR+KE  ++S+VWS C +TWK LRK+ RT+
Sbjct: 66  TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTD 125

Query: 554 IFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNIIGNVVFSKDVF 724
           +F+ +M++ Q  VRE+K  E+VE+L+   G  VKI++ VFGTL+NI GN++FS+++F
Sbjct: 126 LFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIF 182


>gb|AFK73713.1| cytochrome P450 [Papaver somniferum]
          Length = 440

 Score =  168 bits (426), Expect = 2e-39
 Identities = 86/192 (44%), Positives = 130/192 (67%), Gaps = 2/192 (1%)
 Frame = +2

Query: 155 EVITLSLLSFIPFVIVLYLLFKPK--HNLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDL 328
           E++T++L++ + F   LYL+ +    + LPP P+P WP++GN+   LG K   H     L
Sbjct: 2   EIVTVALIAIV-FTTFLYLIVRESSPNGLPPGPKP-WPIVGNLLQ-LGEKP--HSQFAQL 56

Query: 329 AQVHGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIKEMNKHSLVWSN 508
           A+ +G L  + LGT   VVAST  AA EVLK HDR L GR++  SFR+K   ++S+VW+ 
Sbjct: 57  AETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNE 116

Query: 509 CTDTWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLN 688
           C +TWK LRK+ RT++F+ +M++ Q  VRE K  E+V++L+  EG  VKI + +FGTL+N
Sbjct: 117 CNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVN 176

Query: 689 IIGNVVFSKDVF 724
           + GN++FS+D+F
Sbjct: 177 MFGNLIFSQDIF 188


>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
           glaucum]
          Length = 491

 Score =  166 bits (420), Expect = 7e-39
 Identities = 89/192 (46%), Positives = 131/192 (68%), Gaps = 2/192 (1%)
 Frame = +2

Query: 155 EVITLSLLS-FIPFVIVLYLLFKPKH-NLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDL 328
           EV++ +++S F  F+ + ++L   ++ +LPP PRPS P++GN+   LG K   H     L
Sbjct: 2   EVLSAAMVSLFFIFLFIFFILCDSRNKDLPPGPRPS-PIVGNLLQ-LGEKP--HAEFAKL 57

Query: 329 AQVHGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIKEMNKHSLVWSN 508
           A+ +G L  + LG+   VVAS+  AA E+LKT D+ LSGR++  SFR+ E   +S+VWS 
Sbjct: 58  AEKYGELFTLKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSE 117

Query: 509 CTDTWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLN 688
           C + WKLLRK+ RTE+FSP+M++ Q  +RE K  ++V FLR +E + VKI + VF TL+N
Sbjct: 118 CNENWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVN 177

Query: 689 IIGNVVFSKDVF 724
           I GN++FSKDVF
Sbjct: 178 IFGNLIFSKDVF 189


>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
           Full=(S)-N-methylcoclaurine oxidase [C-O
           phenol-coupling]; AltName: Full=CYPLXXX; AltName:
           Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
           cytochrome P-450 CYP80 [Berberis stolonifera]
          Length = 487

 Score =  166 bits (419), Expect = 1e-38
 Identities = 88/183 (48%), Positives = 126/183 (68%), Gaps = 3/183 (1%)
 Frame = +2

Query: 185 IPFVIVLY-LLFKPKH-NLPPSPRPSWPLIGNIPAILGSKTPLHVT-LTDLAQVHGPLMV 355
           I  V +LY LLFKPKH NLPPSP P+WP++G++P ++   +P  +  ++++AQ +GPL+ 
Sbjct: 10  ISLVALLYFLLFKPKHTNLPPSP-PAWPIVGHLPDLISKNSPPFLDYMSNIAQKYGPLIH 68

Query: 356 VTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLR 535
           +  G   ++ AST+EAAMEVL+T+D+ LSGR     FRIK    +S++WS+    WK  R
Sbjct: 69  LKFGLHSSIFASTKEAAMEVLQTNDKVLSGRQPLPCFRIKPHIDYSILWSDSNSYWKKGR 128

Query: 536 KLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNIIGNVVFSK 715
           K+  TEIFS +MLQ QE  RE   G LV F+  + G VV++  ++FG  LN++G+VVFSK
Sbjct: 129 KILHTEIFSQKMLQAQEKNRERVAGNLVNFIMTKVGDVVELRSWLFGCALNVLGHVVFSK 188

Query: 716 DVF 724
           DVF
Sbjct: 189 DVF 191


>ref|XP_004234364.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
           [Solanum lycopersicum]
          Length = 486

 Score =  160 bits (406), Expect = 3e-37
 Identities = 88/188 (46%), Positives = 128/188 (68%), Gaps = 4/188 (2%)
 Frame = +2

Query: 170 SLLSFIPFVIVLYLLFKPKH-NLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDLAQVHGP 346
           S L FIPF ++++    PK  NLPP PRP W L+GN+  I   KTP HV+L   AQ+HGP
Sbjct: 9   SFLIFIPFFLLIFKQLVPKSTNLPPGPRP-WHLLGNLLQI--GKTP-HVSLAKFAQIHGP 64

Query: 347 LMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRM--SFRIKEMNKHSLVWSN-CTD 517
           L+ V LGT   VVAS+  +A E+LK+ DR LS R +    ++ +  +++HS+V+S+  ++
Sbjct: 65  LISVKLGTQLVVVASSPASAAEILKSQDRLLSVRSVPKVATYELSVIDQHSIVFSSDLSN 124

Query: 518 TWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNIIG 697
            WK LR   RT +FSP+ ++ Q  +RE KV E++ FLR R+G+ VKIS+ +FGT+LN +G
Sbjct: 125 QWKFLRAFCRTHLFSPKAVESQAALREKKVSEMMNFLRSRKGETVKISEILFGTILNTLG 184

Query: 698 NVVFSKDV 721
           N+ FSKD+
Sbjct: 185 NLFFSKDL 192


>ref|XP_006358870.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
           2-like [Solanum tuberosum]
          Length = 495

 Score =  159 bits (403), Expect = 7e-37
 Identities = 89/194 (45%), Positives = 128/194 (65%), Gaps = 5/194 (2%)
 Frame = +2

Query: 155 EVITLSLLSFIPFVIVLYLLFKPKH--NLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDL 328
           E++ L     +P    L+ +F+      LPP P P WP+IGNI  +   K P H+TLT+ 
Sbjct: 6   EILFLLPFFLLPLTFFLFKVFRSSKITQLPPGPTP-WPVIGNIFHM--GKMP-HITLTNF 61

Query: 329 AQVHGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHI--RMSFRIKEMNKHSLVW 502
           A+++GPLM + LGT   VV S+  AA+E+LKTHDR LSGRH+   M  +  +++K S+ W
Sbjct: 62  AKIYGPLMSLRLGTQCLVVGSSSSAAIEILKTHDRILSGRHVPNAMPAKRSDLDKISMGW 121

Query: 503 -SNCTDTWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGT 679
            S C + W+ LR L +TE+FS ++L+ Q  +RE KV ELVEFLR +EG+VV I + VF T
Sbjct: 122 NSECHNEWRCLRTLCKTELFSGKVLESQAYLREKKVMELVEFLRSKEGQVVNIGELVFAT 181

Query: 680 LLNIIGNVVFSKDV 721
           +LN++ NV+ SKD+
Sbjct: 182 VLNMLSNVLISKDM 195


>ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
           2-like [Solanum tuberosum]
          Length = 496

 Score =  159 bits (401), Expect = 1e-36
 Identities = 90/198 (45%), Positives = 126/198 (63%), Gaps = 6/198 (3%)
 Frame = +2

Query: 146 QMEEVITLSLLSFIPFVIVLYLLFKPKHN---LPPSPRPSWPLIGNIPAILGSKTPLHVT 316
           Q  E++ L  L   P +  ++ +FK       LPP P P WP+IGNI  I   K P H+T
Sbjct: 3   QQFEILFLLPLFLFPLIFFIFKIFKKSSKITQLPPGPTP-WPVIGNIFQI--GKMP-HIT 58

Query: 317 LTDLAQVHGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIK--EMNKH 490
           LT+ A+ +GPL+ + LGT   VV S+   A+E+ KTHDR LSGRH+  + R K  ++ K 
Sbjct: 59  LTNFAKTYGPLISLRLGTQCLVVGSSPSVAIEIFKTHDRILSGRHVPNAVRSKGSDLEKT 118

Query: 491 SLVW-SNCTDTWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQF 667
           SL W S C   W+ LR L RT++F+ ++L+ Q  +RE KV ELVEFLR +EG+VV I + 
Sbjct: 119 SLGWKSECDSGWRYLRTLCRTDLFAGKVLESQACLREKKVMELVEFLRSKEGQVVNIGEL 178

Query: 668 VFGTLLNIIGNVVFSKDV 721
           VF T+LN++ NV+ SKD+
Sbjct: 179 VFATVLNMLSNVLISKDM 196


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  159 bits (401), Expect = 1e-36
 Identities = 94/205 (45%), Positives = 130/205 (63%), Gaps = 11/205 (5%)
 Frame = +2

Query: 140  NTQMEEVIT--LSLLSFI------PFVIVLYLLFKPKHNLPPSPRPSWPLIGNIPAILGS 295
            NT   + +T  +SL SF+      PF+++L  +F     LPP P P WP+IGN+  +   
Sbjct: 1044 NTMDSDTVTADISLSSFVYALLLLPFLLILKHIFLKPPPLPPGPYP-WPIIGNLLQL--G 1100

Query: 296  KTPLHVTLTDLAQVHGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIK 475
            K P HV L  LA++HGPLM + LGT   VVAS+  AA+EVLKTHDR LSGR++  S  +K
Sbjct: 1101 KNP-HVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVK 1159

Query: 476  E--MNKHSLVWSN-CTDTWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGK 646
            +  +N  SL ++  CT+ WK LR + RTE+FS + ++    +RE KV ELVEFL  +EG+
Sbjct: 1160 DPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGE 1219

Query: 647  VVKISQFVFGTLLNIIGNVVFSKDV 721
            VVK+   VF T+ NI+ N  FS D+
Sbjct: 1220 VVKVMDLVFTTICNILSNTFFSMDL 1244



 Score =  156 bits (394), Expect = 8e-36
 Identities = 93/205 (45%), Positives = 131/205 (63%), Gaps = 11/205 (5%)
 Frame = +2

Query: 140  NTQMEEVIT--LSLLSFI------PFVIVLYLLFKPKHNLPPSPRPSWPLIGNIPAILGS 295
            NT   + +T  +S+ SF+      PF+++L  +F     LPP P P WP+IGN+  +   
Sbjct: 539  NTMDPDTVTADISIFSFLYALLLLPFLVILKHIFLKPPPLPPGPYP-WPIIGNLLQM--G 595

Query: 296  KTPLHVTLTDLAQVHGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIK 475
            K P H  L +LA++HGPLM + LGT   VVAS+  AAMEVLKTHDRALSGR++  S  +K
Sbjct: 596  KNP-HAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVK 654

Query: 476  --EMNKHSLVWS-NCTDTWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGK 646
              ++N  S+V++ +C   WK LR + R E+FS + ++ Q  +RE KV ELVEFL  +EG+
Sbjct: 655  NPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGE 714

Query: 647  VVKISQFVFGTLLNIIGNVVFSKDV 721
            VVK+   VF T+ NI+ N  FS D+
Sbjct: 715  VVKVMDLVFTTICNILSNKFFSMDL 739



 Score =  143 bits (361), Expect = 5e-32
 Identities = 82/196 (41%), Positives = 122/196 (62%), Gaps = 9/196 (4%)
 Frame = +2

Query: 158 VITLSLLSFI------PFVIVLYLLFKPKHNLPPSPRPSWPLIGNIPAILGSKTPLHVTL 319
           +  +SL SF+      PF+++   +F     LPP P P WP+IGN+  + G+   LHV L
Sbjct: 7   IADISLFSFLYPLLLLPFLLIFKHIFLKSPPLPPGPYP-WPIIGNLLQMGGN---LHVKL 62

Query: 320 TDLAQVHGPLMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIK--EMNKHS 493
            +LA+ HGPLM + LGT   VVAS+  AAMEVLKTHDR LSGR++  +  +   ++N  +
Sbjct: 63  ANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSGRYVSTTIPVNSPKLNHLA 122

Query: 494 LVWSN-CTDTWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFV 670
           + ++  C   W+ L+ + R E+FS + ++ +  +RE KV ELVEFL  +EG+VVK+   V
Sbjct: 123 MAFAKVCNSDWRNLKAICRMELFSGKAMESRVELRERKVMELVEFLEKKEGEVVKVMDLV 182

Query: 671 FGTLLNIIGNVVFSKD 718
           + T+ NI+ N  FS D
Sbjct: 183 YTTVCNILSNKFFSID 198


>ref|XP_004234365.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
           [Solanum lycopersicum]
          Length = 486

 Score =  157 bits (398), Expect = 3e-36
 Identities = 88/188 (46%), Positives = 124/188 (65%), Gaps = 4/188 (2%)
 Frame = +2

Query: 170 SLLSFIPFVIVLYLLFKPKH-NLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDLAQVHGP 346
           S L FIP + ++   F PK  NLPP PR SWPL+GNI  I   K P H++L   AQ+HGP
Sbjct: 9   SFLIFIPLLFLIVKQFIPKSGNLPPGPR-SWPLLGNILQI--GKNP-HISLAKFAQIHGP 64

Query: 347 LMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRM--SFRIKEMNKHSLVWSN-CTD 517
           L+ V LGT   VVAS+  +A E+LKT DR LS R       + +  +N+HS+V+S+  ++
Sbjct: 65  LISVKLGTQLVVVASSPASAAEILKTQDRLLSARSPPSVAPYELSVINQHSIVFSSDLSN 124

Query: 518 TWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNIIG 697
            WKLLR   RT +FSP+ ++ Q  +RE KV E++ FL+ ++G+ V IS+ +FGT+LN  G
Sbjct: 125 HWKLLRAFCRTHLFSPKAVESQTALREKKVSEMIHFLKSKKGETVMISEILFGTILNTFG 184

Query: 698 NVVFSKDV 721
           N+ FSKD+
Sbjct: 185 NLFFSKDL 192


>ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
           [Vitis vinifera]
          Length = 515

 Score =  157 bits (396), Expect = 5e-36
 Identities = 88/185 (47%), Positives = 121/185 (65%), Gaps = 3/185 (1%)
 Frame = +2

Query: 176 LSFIPFVIVLYLLFKPKHNLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDLAQVHGPLMV 355
           L  +PF+++L  +F     LPP P P WP+IGN+  +   K P HV L  LA++HGPLM 
Sbjct: 19  LLLLPFLLILKHIFLKPPPLPPGPYP-WPIIGNLLQL--GKNP-HVKLASLAKLHGPLMS 74

Query: 356 VTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIKE--MNKHSLVWSN-CTDTWK 526
           + LGT   VVAS+  AA+EVLKTHDR LSGR++  S  +K+  +N  SL ++  CT+ WK
Sbjct: 75  LRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWK 134

Query: 527 LLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNIIGNVV 706
            LR + RTE+FS + ++    +RE KV ELVEFL  +EG+VVK+   VF T+ NI+ N  
Sbjct: 135 NLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTF 194

Query: 707 FSKDV 721
           FS D+
Sbjct: 195 FSMDL 199


>ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
           2-like [Solanum tuberosum]
          Length = 486

 Score =  155 bits (392), Expect = 1e-35
 Identities = 85/188 (45%), Positives = 127/188 (67%), Gaps = 4/188 (2%)
 Frame = +2

Query: 170 SLLSFIPFVIVLYLLFKPKH-NLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDLAQVHGP 346
           S L FI F +++     PK  NLPP PRP WPL+GNI  I   K+P H++L   A+++GP
Sbjct: 9   SFLIFIAFFLLILKQLIPKSPNLPPGPRP-WPLLGNILQI--GKSP-HISLAQFAKIYGP 64

Query: 347 LMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRM--SFRIKEMNKHSLVWSN-CTD 517
           L+ V LGT   VVAS+ ++A E+LKT DR LS R +    ++ +  +++HS+V+S+  ++
Sbjct: 65  LISVKLGTQLVVVASSPDSAAEILKTQDRLLSVRSVPKVATYELSVIDQHSIVFSSDLSN 124

Query: 518 TWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNIIG 697
            WK LR   RT +FSP+ ++ Q  +RE KV E++ FLR R+G+ VKIS+ +F T+LN +G
Sbjct: 125 HWKFLRAFCRTHLFSPKAVESQAALREKKVSEMIHFLRSRKGETVKISEIMFATILNTLG 184

Query: 698 NVVFSKDV 721
           N+ FSKD+
Sbjct: 185 NLFFSKDL 192


>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 498

 Score =  155 bits (392), Expect = 1e-35
 Identities = 89/188 (47%), Positives = 123/188 (65%), Gaps = 3/188 (1%)
 Frame = +2

Query: 167 LSLLSFIPFVIVLYLLFKPKHNLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDLAQVHGP 346
           L  L  +PF+++L  +F     LPP P P WP+IGN+  +   K P H  L +LA++HGP
Sbjct: 16  LYALLLLPFLVILKHIFLKPPPLPPGPYP-WPIIGNLLQM--GKNP-HAKLANLAKLHGP 71

Query: 347 LMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIK--EMNKHSLVWS-NCTD 517
           LM + LGT   VVAS+  AAMEVLKTHDRALSGR++  S  +K  ++N  S+V++ +C  
Sbjct: 72  LMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNT 131

Query: 518 TWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNIIG 697
            WK LR + R E+FS + ++ Q  +RE KV ELVEFL  +EG+VVK+   VF T+ NI+ 
Sbjct: 132 NWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILS 191

Query: 698 NVVFSKDV 721
           N  FS D+
Sbjct: 192 NKFFSMDL 199


>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
          Length = 498

 Score =  155 bits (392), Expect = 1e-35
 Identities = 89/188 (47%), Positives = 123/188 (65%), Gaps = 3/188 (1%)
 Frame = +2

Query: 167 LSLLSFIPFVIVLYLLFKPKHNLPPSPRPSWPLIGNIPAILGSKTPLHVTLTDLAQVHGP 346
           L  L  +PF+++L  +F     LPP P P WP+IGN+  +   K P H  L +LA++HGP
Sbjct: 16  LYALLLLPFLVILKHIFLKPPPLPPGPYP-WPIIGNLLQM--GKNP-HAKLANLAKLHGP 71

Query: 347 LMVVTLGTAPTVVASTREAAMEVLKTHDRALSGRHIRMSFRIK--EMNKHSLVWS-NCTD 517
           LM + LGT   VVAS+  AAMEVLKTHDRALSGR++  S  +K  ++N  S+V++ +C  
Sbjct: 72  LMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNT 131

Query: 518 TWKLLRKLARTEIFSPRMLQIQEPVRENKVGELVEFLRGREGKVVKISQFVFGTLLNIIG 697
            WK LR + R E+FS + ++ Q  +RE KV ELVEFL  +EG+VVK+   VF T+ NI+ 
Sbjct: 132 NWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILS 191

Query: 698 NVVFSKDV 721
           N  FS D+
Sbjct: 192 NKFFSMDL 199