BLASTX nr result
ID: Cocculus22_contig00025997
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cocculus22_contig00025997
(328 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006342396.1| PREDICTED: poly [ADP-ribose] polymerase 4-li... 132 4e-29
ref|XP_006452827.1| hypothetical protein CICLE_v10007546mg [Citr... 129 6e-28
ref|XP_006452826.1| hypothetical protein CICLE_v10007546mg [Citr... 129 6e-28
gb|EYU25841.1| hypothetical protein MIMGU_mgv1a001817mg [Mimulus... 128 9e-28
ref|XP_006474710.1| PREDICTED: uncharacterized protein LOC102609... 127 2e-27
ref|XP_004235553.1| PREDICTED: uncharacterized protein LOC101253... 125 5e-27
ref|XP_007024661.1| Inter-alpha-trypsin inhibitor heavy chain-re... 125 8e-27
ref|XP_002516254.1| inter-alpha-trypsin inhibitor heavy chain, p... 124 1e-26
ref|XP_004251694.1| PREDICTED: uncharacterized protein LOC101250... 124 2e-26
ref|XP_002271630.1| PREDICTED: uncharacterized protein LOC100244... 124 2e-26
ref|XP_006345262.1| PREDICTED: uncharacterized protein LOC102582... 123 2e-26
ref|XP_002277641.1| PREDICTED: inter-alpha-trypsin inhibitor hea... 123 2e-26
ref|XP_006587053.1| PREDICTED: uncharacterized protein LOC100820... 123 3e-26
ref|XP_003595328.1| Inter-alpha-trypsin inhibitor heavy chain H3... 123 3e-26
ref|XP_006426697.1| hypothetical protein CICLE_v10024980mg [Citr... 122 7e-26
ref|XP_004488096.1| PREDICTED: inter-alpha-trypsin inhibitor hea... 122 7e-26
ref|XP_004488095.1| PREDICTED: inter-alpha-trypsin inhibitor hea... 122 7e-26
ref|XP_007138646.1| hypothetical protein PHAVU_009G226200g [Phas... 121 1e-25
ref|XP_007138645.1| hypothetical protein PHAVU_009G226200g [Phas... 121 1e-25
ref|XP_006465891.1| PREDICTED: inter-alpha-trypsin inhibitor hea... 120 2e-25
>ref|XP_006342396.1| PREDICTED: poly [ADP-ribose] polymerase 4-like [Solanum tuberosum]
Length = 752
Score = 132 bits (333), Expect = 4e-29
Identities = 70/108 (64%), Positives = 80/108 (74%)
Frame = -1
Query: 325 RKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSLV 146
RKVFRKEVVF+VD S SMKGKP+E K AL TALSKL+ +D F IIAFN E + FSSSL
Sbjct: 320 RKVFRKEVVFVVDISGSMKGKPIEDTKQALCTALSKLDSQDLFNIIAFNSEKYLFSSSLE 379
Query: 145 LATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
LATK++IENATQW +NFIAGG T+IL PL QA+EM IF
Sbjct: 380 LATKKSIENATQWIGTNFIAGGGTNILNPLTQAMEMFSYTQQHIPIIF 427
>ref|XP_006452827.1| hypothetical protein CICLE_v10007546mg [Citrus clementina]
gi|557556053|gb|ESR66067.1| hypothetical protein
CICLE_v10007546mg [Citrus clementina]
Length = 758
Score = 129 bits (323), Expect = 6e-28
Identities = 65/109 (59%), Positives = 81/109 (74%)
Frame = -1
Query: 328 SRKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSL 149
SRKVF+K+V+F+VD S+SM+GKPLE KNAL ALSKL+P D+F IIAFNGE++ FS+S+
Sbjct: 321 SRKVFKKDVIFIVDISKSMQGKPLEDTKNALAVALSKLDPGDSFNIIAFNGETYLFSTSM 380
Query: 148 VLATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
LATKE +E A QW NFIAGG T+I PL +A+EML IF
Sbjct: 381 ELATKEAVERAHQWIGINFIAGGSTNICAPLTKAVEMLTNSRGSIPIIF 429
>ref|XP_006452826.1| hypothetical protein CICLE_v10007546mg [Citrus clementina]
gi|557556052|gb|ESR66066.1| hypothetical protein
CICLE_v10007546mg [Citrus clementina]
Length = 613
Score = 129 bits (323), Expect = 6e-28
Identities = 65/109 (59%), Positives = 81/109 (74%)
Frame = -1
Query: 328 SRKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSL 149
SRKVF+K+V+F+VD S+SM+GKPLE KNAL ALSKL+P D+F IIAFNGE++ FS+S+
Sbjct: 321 SRKVFKKDVIFIVDISKSMQGKPLEDTKNALAVALSKLDPGDSFNIIAFNGETYLFSTSM 380
Query: 148 VLATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
LATKE +E A QW NFIAGG T+I PL +A+EML IF
Sbjct: 381 ELATKEAVERAHQWIGINFIAGGSTNICAPLTKAVEMLTNSRGSIPIIF 429
>gb|EYU25841.1| hypothetical protein MIMGU_mgv1a001817mg [Mimulus guttatus]
Length = 755
Score = 128 bits (321), Expect = 9e-28
Identities = 66/109 (60%), Positives = 80/109 (73%)
Frame = -1
Query: 328 SRKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSL 149
S KVFRKEVVF+VD S SM+G+ LE KNALF AL+KL+P+D+F +IAFNGE + FSS+L
Sbjct: 321 SEKVFRKEVVFVVDISGSMRGRTLEDTKNALFAALAKLDPKDSFNVIAFNGEIYLFSSAL 380
Query: 148 VLATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
AT E IEN TQW + NF+AGG T+IL PLNQA+ ML IF
Sbjct: 381 EPATSEAIENVTQWINMNFVAGGGTNILLPLNQAMNMLSDSCNSMPIIF 429
>ref|XP_006474710.1| PREDICTED: uncharacterized protein LOC102609180 [Citrus sinensis]
Length = 759
Score = 127 bits (319), Expect = 2e-27
Identities = 64/109 (58%), Positives = 80/109 (73%)
Frame = -1
Query: 328 SRKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSL 149
SRKVF+K+V+F+VD S SM+GKPLE KNAL ALSKL+P D+F I+AFNGE++ FS+S+
Sbjct: 321 SRKVFKKDVIFIVDISGSMQGKPLEDTKNALAVALSKLDPGDSFNIVAFNGETYLFSTSM 380
Query: 148 VLATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
LATKE +E A QW NFIAGG T+I PL +A+EML IF
Sbjct: 381 ELATKEAVERAHQWIGINFIAGGSTNICAPLTKAVEMLTNSRGSIPIIF 429
>ref|XP_004235553.1| PREDICTED: uncharacterized protein LOC101253877 [Solanum
lycopersicum]
Length = 744
Score = 125 bits (315), Expect = 5e-27
Identities = 64/96 (66%), Positives = 77/96 (80%)
Frame = -1
Query: 322 KVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSLVL 143
KVFRKEVV++VD S SM+G+PLE++K+AL ALSKL+P D F IIAFNG+S FSSSL+L
Sbjct: 318 KVFRKEVVYVVDISASMQGRPLENIKSALLAALSKLSPADTFNIIAFNGKSLLFSSSLML 377
Query: 142 ATKENIENATQWTSSNFIAGGLTDILQPLNQAIEML 35
+ KE+I ATQW NF+A G TDI PLNQAIEML
Sbjct: 378 SGKESIGKATQWIDQNFVAEGSTDISLPLNQAIEML 413
>ref|XP_007024661.1| Inter-alpha-trypsin inhibitor heavy chain-related, putative
[Theobroma cacao] gi|508780027|gb|EOY27283.1|
Inter-alpha-trypsin inhibitor heavy chain-related,
putative [Theobroma cacao]
Length = 752
Score = 125 bits (313), Expect = 8e-27
Identities = 63/107 (58%), Positives = 80/107 (74%)
Frame = -1
Query: 322 KVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSLVL 143
KVFRKEVVF++DTSESM+G+P+ESVKNAL LSKLNP+D+F IIAFN E H FSS++VL
Sbjct: 320 KVFRKEVVFVIDTSESMQGRPIESVKNALLALLSKLNPQDSFNIIAFNSEIHLFSSTMVL 379
Query: 142 ATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
AT +I NAT W S+N A G T+++ PL QA+++L IF
Sbjct: 380 ATHGSILNATHWLSNNLTADGGTNMMLPLKQAMKLLSDASDSIPLIF 426
>ref|XP_002516254.1| inter-alpha-trypsin inhibitor heavy chain, putative [Ricinus
communis] gi|223544740|gb|EEF46256.1|
inter-alpha-trypsin inhibitor heavy chain, putative
[Ricinus communis]
Length = 755
Score = 124 bits (312), Expect = 1e-26
Identities = 62/107 (57%), Positives = 81/107 (75%)
Frame = -1
Query: 322 KVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSLVL 143
KVFRKE+VF+VD S SM+GKPLE +KNA+ AL+KLNP+D+F IIAFNGE++ FSS + L
Sbjct: 321 KVFRKEIVFIVDISGSMEGKPLEGMKNAMSGALAKLNPKDSFNIIAFNGETYLFSSLMEL 380
Query: 142 ATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
AT++ +E A +W + NFIAGG T+I PLNQA+EM+ IF
Sbjct: 381 ATEKTVERAVEWMNLNFIAGGGTNISVPLNQAMEMVSNTQGSLPVIF 427
>ref|XP_004251694.1| PREDICTED: uncharacterized protein LOC101250962 [Solanum
lycopersicum]
Length = 753
Score = 124 bits (310), Expect = 2e-26
Identities = 64/108 (59%), Positives = 76/108 (70%)
Frame = -1
Query: 325 RKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSLV 146
RKVFRKEV+F+VD S SMKGKP++ +K AL ALSKL+ +D F IIAFN E + FSSSL
Sbjct: 322 RKVFRKEVIFVVDISGSMKGKPIDGIKQALSGALSKLDSQDLFNIIAFNNEEYQFSSSLE 381
Query: 145 LATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
+ATKE I+NA QW NFI GG T+IL P+ QAI ML IF
Sbjct: 382 VATKEAIDNAIQWIDMNFIVGGSTNILNPMKQAIGMLSDVGESMPIIF 429
>ref|XP_002271630.1| PREDICTED: uncharacterized protein LOC100244700 [Vitis vinifera]
gi|297742983|emb|CBI35850.3| unnamed protein product
[Vitis vinifera]
Length = 747
Score = 124 bits (310), Expect = 2e-26
Identities = 64/109 (58%), Positives = 81/109 (74%)
Frame = -1
Query: 328 SRKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSL 149
S KVFRKEVVF+VD S SM+G+PLE+ K+A+ AL LN +D+F IIAFNG++ FSS++
Sbjct: 320 SGKVFRKEVVFIVDISRSMRGRPLENTKDAVLAALLNLNTQDSFNIIAFNGDTCLFSSTM 379
Query: 148 VLATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
VLATKE + NATQW ++NF A G T+IL PL+QAIEML IF
Sbjct: 380 VLATKEEVGNATQWINTNFNAEGGTNILNPLSQAIEMLTKTSGSMPLIF 428
>ref|XP_006345262.1| PREDICTED: uncharacterized protein LOC102582049 [Solanum tuberosum]
Length = 754
Score = 123 bits (309), Expect = 2e-26
Identities = 65/108 (60%), Positives = 76/108 (70%)
Frame = -1
Query: 325 RKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSLV 146
RKVFRKEV+F+VD S SMKGKP++ K AL ALSKL+ +D F IIAFN E + FSSSL
Sbjct: 322 RKVFRKEVIFVVDISGSMKGKPIDDSKQALSVALSKLDSQDLFNIIAFNNEEYLFSSSLE 381
Query: 145 LATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
+ATKE I+NA QW NFIAGG T+IL P+ QAI ML IF
Sbjct: 382 VATKEAIDNAIQWIDMNFIAGGSTNILNPMKQAIGMLSNAGESMPIIF 429
>ref|XP_002277641.1| PREDICTED: inter-alpha-trypsin inhibitor heavy chain H3 [Vitis
vinifera] gi|297738541|emb|CBI27786.3| unnamed protein
product [Vitis vinifera]
Length = 756
Score = 123 bits (309), Expect = 2e-26
Identities = 67/107 (62%), Positives = 78/107 (72%)
Frame = -1
Query: 322 KVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSLVL 143
KVFRKEVVF+VD S SM+GK LE KNAL ALSKL+ +D+F IIAFNGE FSSS+ L
Sbjct: 320 KVFRKEVVFVVDISGSMRGKLLEDTKNALSAALSKLDSKDSFSIIAFNGEIFIFSSSVQL 379
Query: 142 ATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
ATKE IENA QW S NFIAGG T+IL P+N+A+E+ IF
Sbjct: 380 ATKEAIENAIQWISMNFIAGGDTNILLPMNKAMELFSHSPGSIPIIF 426
>ref|XP_006587053.1| PREDICTED: uncharacterized protein LOC100820133 [Glycine max]
Length = 759
Score = 123 bits (308), Expect = 3e-26
Identities = 61/108 (56%), Positives = 78/108 (72%)
Frame = -1
Query: 325 RKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSLV 146
RK+FRK++VF++D S SM+GK ++ KNAL ALSKLNP D+F IIAFNGE + FS S+
Sbjct: 324 RKIFRKDIVFVIDISGSMRGKLIDHTKNALSAALSKLNPHDSFSIIAFNGEIYQFSKSME 383
Query: 145 LATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
LA+K+ +E A +W + NFIAGG T+IL PLN AIEML IF
Sbjct: 384 LASKDAVERAIEWINMNFIAGGDTNILHPLNTAIEMLSDAQSSVPIIF 431
>ref|XP_003595328.1| Inter-alpha-trypsin inhibitor heavy chain H3 [Medicago truncatula]
gi|355484376|gb|AES65579.1| Inter-alpha-trypsin
inhibitor heavy chain H3 [Medicago truncatula]
Length = 751
Score = 123 bits (308), Expect = 3e-26
Identities = 60/109 (55%), Positives = 80/109 (73%)
Frame = -1
Query: 328 SRKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSL 149
SRKVFRK++VF++D S SM+GK ++ KNAL ALSKL+P+D+F IIAFNGE + FS+S+
Sbjct: 321 SRKVFRKDIVFVIDISGSMRGKLIDDTKNALSAALSKLDPDDSFSIIAFNGEIYQFSTSM 380
Query: 148 VLATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
LA+K+ +E A +W NF+AGG T+IL PLN A+EML IF
Sbjct: 381 ELASKDAVERAVEWIGINFVAGGDTNILHPLNTALEMLSDGRRSVPIIF 429
>ref|XP_006426697.1| hypothetical protein CICLE_v10024980mg [Citrus clementina]
gi|557528687|gb|ESR39937.1| hypothetical protein
CICLE_v10024980mg [Citrus clementina]
Length = 742
Score = 122 bits (305), Expect = 7e-26
Identities = 67/109 (61%), Positives = 80/109 (73%)
Frame = -1
Query: 328 SRKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSL 149
SRKVFRK+VVFLVD S SM+G LE KNAL +LSKLNP+D+F IIAFNGE+H FSSS+
Sbjct: 320 SRKVFRKDVVFLVDVSGSMQGVLLEQTKNALSASLSKLNPQDSFNIIAFNGETHLFSSSM 379
Query: 148 VLATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
LA++ I NATQW SS +AGG T+IL PL QAI++L IF
Sbjct: 380 KLASQGTITNATQWLSS-LVAGGGTNILLPLKQAIKLLSDTSESIPLIF 427
>ref|XP_004488096.1| PREDICTED: inter-alpha-trypsin inhibitor heavy chain H3-like
isoform X2 [Cicer arietinum]
Length = 600
Score = 122 bits (305), Expect = 7e-26
Identities = 60/109 (55%), Positives = 82/109 (75%)
Frame = -1
Query: 328 SRKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSL 149
S+KVFRK++VF++D S SM+GK ++ KNAL +ALSKL+ +D+F IIAFNGE++ FS+S+
Sbjct: 168 SKKVFRKDIVFVIDISGSMRGKLIDDTKNALQSALSKLHHDDSFSIIAFNGETYQFSTSM 227
Query: 148 VLATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
LA+K+ +E A QW + NF+AGG T+IL PLN AIEML IF
Sbjct: 228 ELASKDAVERAIQWININFVAGGDTNILHPLNMAIEMLSDAQRSVPIIF 276
>ref|XP_004488095.1| PREDICTED: inter-alpha-trypsin inhibitor heavy chain H3-like
isoform X1 [Cicer arietinum]
Length = 752
Score = 122 bits (305), Expect = 7e-26
Identities = 60/109 (55%), Positives = 82/109 (75%)
Frame = -1
Query: 328 SRKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSL 149
S+KVFRK++VF++D S SM+GK ++ KNAL +ALSKL+ +D+F IIAFNGE++ FS+S+
Sbjct: 320 SKKVFRKDIVFVIDISGSMRGKLIDDTKNALQSALSKLHHDDSFSIIAFNGETYQFSTSM 379
Query: 148 VLATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
LA+K+ +E A QW + NF+AGG T+IL PLN AIEML IF
Sbjct: 380 ELASKDAVERAIQWININFVAGGDTNILHPLNMAIEMLSDAQRSVPIIF 428
>ref|XP_007138646.1| hypothetical protein PHAVU_009G226200g [Phaseolus vulgaris]
gi|561011733|gb|ESW10640.1| hypothetical protein
PHAVU_009G226200g [Phaseolus vulgaris]
Length = 761
Score = 121 bits (303), Expect = 1e-25
Identities = 56/109 (51%), Positives = 81/109 (74%)
Frame = -1
Query: 328 SRKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSL 149
S+K+FRK+++F++D S SM+GK ++ +NAL AL+KLNP+D+F IIAFNG+ + FS+S+
Sbjct: 323 SKKIFRKDIIFVIDVSGSMRGKLIDDTRNALSVALAKLNPDDSFSIIAFNGDIYQFSASM 382
Query: 148 VLATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
LA+K+ +E A +W + NF+AGG T+IL PLN AIEML IF
Sbjct: 383 ELASKDAVERAIEWINMNFVAGGDTNILHPLNTAIEMLSHAQSSVPIIF 431
>ref|XP_007138645.1| hypothetical protein PHAVU_009G226200g [Phaseolus vulgaris]
gi|561011732|gb|ESW10639.1| hypothetical protein
PHAVU_009G226200g [Phaseolus vulgaris]
Length = 600
Score = 121 bits (303), Expect = 1e-25
Identities = 56/109 (51%), Positives = 81/109 (74%)
Frame = -1
Query: 328 SRKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSL 149
S+K+FRK+++F++D S SM+GK ++ +NAL AL+KLNP+D+F IIAFNG+ + FS+S+
Sbjct: 162 SKKIFRKDIIFVIDVSGSMRGKLIDDTRNALSVALAKLNPDDSFSIIAFNGDIYQFSASM 221
Query: 148 VLATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
LA+K+ +E A +W + NF+AGG T+IL PLN AIEML IF
Sbjct: 222 ELASKDAVERAIEWINMNFVAGGDTNILHPLNTAIEMLSHAQSSVPIIF 270
>ref|XP_006465891.1| PREDICTED: inter-alpha-trypsin inhibitor heavy chain H3-like
[Citrus sinensis]
Length = 751
Score = 120 bits (301), Expect = 2e-25
Identities = 66/109 (60%), Positives = 80/109 (73%)
Frame = -1
Query: 328 SRKVFRKEVVFLVDTSESMKGKPLESVKNALFTALSKLNPEDAFGIIAFNGESHSFSSSL 149
SRKVFRK+VVFLVD S SM+G LE KNAL +LSKLNP+D+F IIAFNGE+H FSSS+
Sbjct: 320 SRKVFRKDVVFLVDVSGSMQGVLLEQTKNALSASLSKLNPQDSFNIIAFNGETHLFSSSM 379
Query: 148 VLATKENIENATQWTSSNFIAGGLTDILQPLNQAIEMLXXXXXXXSTIF 2
LA++ I NATQW SS +AGG T+IL P+ QAI++L IF
Sbjct: 380 KLASQGTIINATQWLSS-LVAGGGTNILLPIKQAIKLLSDTSESIPLIF 427