BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cocculus22_contig00014917
         (258 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003525948.1| PREDICTED: lecithin-cholesterol acyltransfer...   150   1e-34
ref|XP_007136777.1| hypothetical protein PHAVU_009G073200g [Phas...   144   1e-32
emb|CBI19376.3| unnamed protein product [Vitis vinifera]              142   6e-32
ref|XP_002283444.1| PREDICTED: lecithin-cholesterol acyltransfer...   142   6e-32
emb|CAN70371.1| hypothetical protein VITISV_035399 [Vitis vinifera]   142   6e-32
ref|XP_002285416.1| PREDICTED: lecithin-cholesterol acyltransfer...   141   8e-32
ref|XP_007018457.1| Alpha/beta-Hydrolases superfamily protein [T...   139   3e-31
ref|XP_007224068.1| hypothetical protein PRUPE_ppa015352mg [Prun...   137   1e-30
gb|AAQ10316.1| lecithine cholesterol acyltransferase-like protei...   137   1e-30
ref|XP_006472325.1| PREDICTED: lecithin-cholesterol acyltransfer...   137   2e-30
ref|XP_004290985.1| PREDICTED: lecithin-cholesterol acyltransfer...   136   4e-30
ref|XP_004501509.1| PREDICTED: lecithin-cholesterol acyltransfer...   135   6e-30
ref|XP_006433658.1| hypothetical protein CICLE_v10001149mg [Citr...   134   1e-29
gb|EXB63112.1| Lecithin-cholesterol acyltransferase-like 1 [Moru...   132   7e-29
ref|XP_002320681.2| lecithin-cholesterol acyl transferase family...   132   7e-29
ref|XP_006374825.1| hypothetical protein POPTR_0014s01470g [Popu...   132   7e-29
ref|NP_564286.1| Lecithin-cholesterol acyltransferase-like 1 [Ar...   131   1e-28
gb|AFK36900.1| unknown [Lotus japonicus]                              131   1e-28
ref|XP_002890730.1| lecithin:cholesterol acyltransferase family ...   131   1e-28
ref|XP_006307463.1| hypothetical protein CARUB_v10009090mg [Caps...   130   2e-28

>ref|XP_003525948.1| PREDICTED: lecithin-cholesterol acyltransferase-like 1-like
           [Glycine max]
          Length = 443

 Score =  150 bits (380), Expect = 1e-34
 Identities = 68/86 (79%), Positives = 80/86 (93%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           Q LGY +G+TLFGAPYDFRYGLAA+GHPS +G++FL++LK LIE+AS+SNNG+PVILLSH
Sbjct: 152 QKLGYADGETLFGAPYDFRYGLAAEGHPSQVGSKFLKDLKNLIEEASNSNNGKPVILLSH 211

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGLFVL LLNRNPPSWR+ FIKHF
Sbjct: 212 SLGGLFVLQLLNRNPPSWRKKFIKHF 237


>ref|XP_007136777.1| hypothetical protein PHAVU_009G073200g [Phaseolus vulgaris]
           gi|561009864|gb|ESW08771.1| hypothetical protein
           PHAVU_009G073200g [Phaseolus vulgaris]
          Length = 442

 Score =  144 bits (363), Expect = 1e-32
 Identities = 66/86 (76%), Positives = 77/86 (89%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           Q LGY +G+TLFGAPYDFRYGLAA+GHPS +G++FL++LK LIE+AS+SN G+PVILLSH
Sbjct: 151 QKLGYADGETLFGAPYDFRYGLAAEGHPSQVGSKFLKDLKNLIEEASNSNGGKPVILLSH 210

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGLFVL LL RNPPSWR  FIKHF
Sbjct: 211 SLGGLFVLQLLIRNPPSWREKFIKHF 236


>emb|CBI19376.3| unnamed protein product [Vitis vinifera]
          Length = 514

 Score =  142 bits (357), Expect = 6e-32
 Identities = 64/84 (76%), Positives = 76/84 (90%)
 Frame = -1

Query: 252 LGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSHSL 73
           +GY +G+TLFGAPYDFRYGLAA GHPS +G++FL++LK LIE+AS SN G+PVIL+SHSL
Sbjct: 146 MGYVDGKTLFGAPYDFRYGLAADGHPSRVGSKFLEDLKDLIEKASTSNGGKPVILVSHSL 205

Query: 72  GGLFVLHLLNRNPPSWRRHFIKHF 1
           GGLFVL LLNRNPPSWR+ FIKHF
Sbjct: 206 GGLFVLQLLNRNPPSWRQKFIKHF 229


>ref|XP_002283444.1| PREDICTED: lecithin-cholesterol acyltransferase-like 1-like [Vitis
           vinifera]
          Length = 431

 Score =  142 bits (357), Expect = 6e-32
 Identities = 64/84 (76%), Positives = 76/84 (90%)
 Frame = -1

Query: 252 LGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSHSL 73
           +GY +G+TLFGAPYDFRYGLAA GHPS +G++FL++LK LIE+AS SN G+PVIL+SHSL
Sbjct: 144 MGYVDGKTLFGAPYDFRYGLAADGHPSRVGSKFLEDLKDLIEKASTSNGGKPVILVSHSL 203

Query: 72  GGLFVLHLLNRNPPSWRRHFIKHF 1
           GGLFVL LLNRNPPSWR+ FIKHF
Sbjct: 204 GGLFVLQLLNRNPPSWRQKFIKHF 227


>emb|CAN70371.1| hypothetical protein VITISV_035399 [Vitis vinifera]
          Length = 919

 Score =  142 bits (357), Expect = 6e-32
 Identities = 64/84 (76%), Positives = 76/84 (90%)
 Frame = -1

Query: 252 LGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSHSL 73
           +GY +G+TLFGAPYDFRYGLAA GHPS +G++FL++LK LIE+AS SN G+PVIL+SHSL
Sbjct: 146 MGYVDGKTLFGAPYDFRYGLAADGHPSRVGSKFLEDLKDLIEKASTSNGGKPVILVSHSL 205

Query: 72  GGLFVLHLLNRNPPSWRRHFIKHF 1
           GGLFVL LLNRNPPSWR+ FIKHF
Sbjct: 206 GGLFVLQLLNRNPPSWRQKFIKHF 229



 Score =  141 bits (356), Expect = 8e-32
 Identities = 63/86 (73%), Positives = 77/86 (89%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + +GY +G+TLFGAPYDFRYGLAA GHPS +G++FL++LK LIE+AS SN G+PVIL+SH
Sbjct: 625 EQMGYVDGETLFGAPYDFRYGLAADGHPSRVGSKFLEDLKDLIEKASTSNGGKPVILVSH 684

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGLFVL LLNRNPP+WR+ FIKHF
Sbjct: 685 SLGGLFVLQLLNRNPPAWRQKFIKHF 710


>ref|XP_002285416.1| PREDICTED: lecithin-cholesterol acyltransferase-like 1 [Vitis
           vinifera]
          Length = 426

 Score =  141 bits (356), Expect = 8e-32
 Identities = 63/86 (73%), Positives = 77/86 (89%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + +GY +G+TLFGAPYDFRYGLAA GHPS +G++FL++LK LIE+AS SN G+PVIL+SH
Sbjct: 142 EQMGYVDGETLFGAPYDFRYGLAADGHPSRVGSKFLEDLKDLIEKASTSNGGKPVILVSH 201

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGLFVL LLNRNPP+WR+ FIKHF
Sbjct: 202 SLGGLFVLQLLNRNPPAWRQKFIKHF 227


>ref|XP_007018457.1| Alpha/beta-Hydrolases superfamily protein [Theobroma cacao]
           gi|508723785|gb|EOY15682.1| Alpha/beta-Hydrolases
           superfamily protein [Theobroma cacao]
          Length = 428

 Score =  139 bits (351), Expect = 3e-31
 Identities = 63/86 (73%), Positives = 76/86 (88%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + +GY  G+TLFGAPYDFRYGLAA+GHPSH+G++FL++LK LIE+AS SN G+PVILLSH
Sbjct: 142 EEIGYVSGETLFGAPYDFRYGLAAEGHPSHVGSKFLRDLKDLIEKASASNGGKPVILLSH 201

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGL+VL LLNRN  SWR+ FIKHF
Sbjct: 202 SLGGLYVLQLLNRNSASWRQRFIKHF 227


>ref|XP_007224068.1| hypothetical protein PRUPE_ppa015352mg [Prunus persica]
           gi|462421004|gb|EMJ25267.1| hypothetical protein
           PRUPE_ppa015352mg [Prunus persica]
          Length = 421

 Score =  137 bits (346), Expect = 1e-30
 Identities = 62/86 (72%), Positives = 73/86 (84%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + +GY   +TLFGAPYDFRYGLA + HP+H+G++FLQ+LK LIE AS SN G+PVIL+SH
Sbjct: 130 ENIGYVNEKTLFGAPYDFRYGLAPEDHPAHVGSKFLQDLKDLIENASTSNGGRPVILVSH 189

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGLF LHLLNRN PSWRR FIKHF
Sbjct: 190 SLGGLFALHLLNRNTPSWRRKFIKHF 215


>gb|AAQ10316.1| lecithine cholesterol acyltransferase-like protein [Medicago
           truncatula]
          Length = 449

 Score =  137 bits (346), Expect = 1e-30
 Identities = 63/86 (73%), Positives = 74/86 (86%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + LGY +GQ LFGAPYDFRYGLAA  HPS +G++FL +LK LIE+AS+SN G+PVIL+SH
Sbjct: 153 EQLGYIDGQNLFGAPYDFRYGLAAPSHPSQVGSKFLNDLKNLIEKASNSNGGKPVILVSH 212

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGLFVL LLNRNP SWR+ FIKHF
Sbjct: 213 SLGGLFVLELLNRNPSSWRKKFIKHF 238


>ref|XP_006472325.1| PREDICTED: lecithin-cholesterol acyltransferase-like 1-like [Citrus
           sinensis]
          Length = 447

 Score =  137 bits (345), Expect = 2e-30
 Identities = 63/86 (73%), Positives = 75/86 (87%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + +GY  G+TLFGAPYDFRYGLAA GHPS +G++FLQ+LK LIE+AS+SN G+PVIL+SH
Sbjct: 160 ERVGYVTGETLFGAPYDFRYGLAAGGHPSRVGSKFLQDLKDLIEKASNSNGGKPVILVSH 219

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGLFVL LLNRNP  WR+ FIKHF
Sbjct: 220 SLGGLFVLQLLNRNPLPWRKKFIKHF 245


>ref|XP_004290985.1| PREDICTED: lecithin-cholesterol acyltransferase-like 1-like
           [Fragaria vesca subsp. vesca]
          Length = 430

 Score =  136 bits (342), Expect = 4e-30
 Identities = 59/86 (68%), Positives = 76/86 (88%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + +GY +G+T+FGAPYDFRYGLA + HPSH+G++FL++LK L+E+AS+SN G+PVIL+SH
Sbjct: 140 ENIGYVDGKTMFGAPYDFRYGLAPKDHPSHVGSKFLKDLKYLVEKASNSNGGKPVILVSH 199

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGLF L LLNRN PSWR+ FIKHF
Sbjct: 200 SLGGLFALQLLNRNKPSWRQKFIKHF 225


>ref|XP_004501509.1| PREDICTED: lecithin-cholesterol acyltransferase-like 1-like [Cicer
           arietinum]
          Length = 449

 Score =  135 bits (340), Expect = 6e-30
 Identities = 62/86 (72%), Positives = 74/86 (86%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + LGY +G+ LFGAPYDFRYGLAA GH S +G++FL +LK LIE+AS+SN G+PVIL+SH
Sbjct: 150 EQLGYIDGKNLFGAPYDFRYGLAAPGHSSQVGSKFLNDLKNLIEKASNSNGGKPVILVSH 209

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGLFVL LLNRNP SWR+ FIKHF
Sbjct: 210 SLGGLFVLELLNRNPSSWRKKFIKHF 235


>ref|XP_006433658.1| hypothetical protein CICLE_v10001149mg [Citrus clementina]
           gi|557535780|gb|ESR46898.1| hypothetical protein
           CICLE_v10001149mg [Citrus clementina]
          Length = 446

 Score =  134 bits (337), Expect = 1e-29
 Identities = 62/86 (72%), Positives = 74/86 (86%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + +GY  G+TLFGAPYDFRYGLAA GHPS +G++FLQ+LK LIE+AS+SN G+PVIL+SH
Sbjct: 159 ERVGYVTGETLFGAPYDFRYGLAAGGHPSRVGSKFLQDLKDLIEKASNSNGGKPVILVSH 218

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGL VL LLNRNP  WR+ FIKHF
Sbjct: 219 SLGGLCVLQLLNRNPLPWRKKFIKHF 244


>gb|EXB63112.1| Lecithin-cholesterol acyltransferase-like 1 [Morus notabilis]
          Length = 456

 Score =  132 bits (331), Expect = 7e-29
 Identities = 60/85 (70%), Positives = 71/85 (83%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + +GY  G+TLFGAPYDFRYGLA  GHP H+G++FLQ LK LIE+AS SN G+PVIL+SH
Sbjct: 155 EQIGYENGKTLFGAPYDFRYGLAPDGHPCHVGSKFLQELKELIEKASSSNGGKPVILVSH 214

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKH 4
           SLGGLFVL LLNRN  SWR+ F+KH
Sbjct: 215 SLGGLFVLQLLNRNTLSWRQKFVKH 239


>ref|XP_002320681.2| lecithin-cholesterol acyl transferase family protein [Populus
           trichocarpa] gi|550323115|gb|EEE98996.2|
           lecithin-cholesterol acyl transferase family protein
           [Populus trichocarpa]
          Length = 433

 Score =  132 bits (331), Expect = 7e-29
 Identities = 57/86 (66%), Positives = 73/86 (84%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + +GY  G+TLFGAPYDFRYGLAA+GHPS +G++FL +LK L+E+AS  N G+PVI++SH
Sbjct: 148 EEIGYVSGETLFGAPYDFRYGLAAEGHPSRVGSKFLLDLKDLVEKASRDNGGKPVIIVSH 207

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGLF L LLN+NP SWR+ +IKHF
Sbjct: 208 SLGGLFALQLLNKNPISWRKKYIKHF 233


>ref|XP_006374825.1| hypothetical protein POPTR_0014s01470g [Populus trichocarpa]
           gi|118487414|gb|ABK95535.1| unknown [Populus
           trichocarpa] gi|550323116|gb|ERP52622.1| hypothetical
           protein POPTR_0014s01470g [Populus trichocarpa]
          Length = 426

 Score =  132 bits (331), Expect = 7e-29
 Identities = 57/86 (66%), Positives = 73/86 (84%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + +GY  G+TLFGAPYDFRYGLAA+GHPS +G++FL +LK L+E+AS  N G+PVI++SH
Sbjct: 141 EEIGYVSGETLFGAPYDFRYGLAAEGHPSRVGSKFLLDLKDLVEKASRDNGGKPVIIVSH 200

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGLF L LLN+NP SWR+ +IKHF
Sbjct: 201 SLGGLFALQLLNKNPISWRKKYIKHF 226


>ref|NP_564286.1| Lecithin-cholesterol acyltransferase-like 1 [Arabidopsis thaliana]
           gi|75173439|sp|Q9FZI8.1|LCAT1_ARATH RecName:
           Full=Lecithin-cholesterol acyltransferase-like 1
           gi|9802537|gb|AAF99739.1|AC004557_18 F17L21.27
           [Arabidopsis thaliana]
           gi|13605855|gb|AAK32913.1|AF367326_1 At1g27480/F17L21_28
           [Arabidopsis thaliana] gi|21593031|gb|AAM64980.1|
           unknown [Arabidopsis thaliana]
           gi|22137198|gb|AAM91444.1| At1g27480/F17L21_28
           [Arabidopsis thaliana] gi|33339740|gb|AAQ14348.1|
           lecithin cholesterol acyltransferase [Arabidopsis
           thaliana] gi|39777538|gb|AAR31109.1| lecithine
           cholesterol acyltransferase-like protein [Arabidopsis
           thaliana] gi|332192716|gb|AEE30837.1|
           Lecithin-cholesterol acyltransferase-like 1 [Arabidopsis
           thaliana]
          Length = 432

 Score =  131 bits (329), Expect = 1e-28
 Identities = 59/83 (71%), Positives = 67/83 (80%)
 Frame = -1

Query: 249 GYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSHSLG 70
           GY   QT+ GAPYDFRYGLAA GHPS + +QFLQ+LK L+E+ S  N G+PVILLSHSLG
Sbjct: 152 GYVNDQTILGAPYDFRYGLAASGHPSRVASQFLQDLKQLVEKTSSENEGKPVILLSHSLG 211

Query: 69  GLFVLHLLNRNPPSWRRHFIKHF 1
           GLFVLH LNR  PSWRR +IKHF
Sbjct: 212 GLFVLHFLNRTTPSWRRKYIKHF 234


>gb|AFK36900.1| unknown [Lotus japonicus]
          Length = 441

 Score =  131 bits (329), Expect = 1e-28
 Identities = 60/86 (69%), Positives = 74/86 (86%)
 Frame = -1

Query: 258 QTLGYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSH 79
           + LGY +G+TLFGAPYDFRYGLAA GHPS +GT+FL +LK+LIE+AS SN G+PVIL+SH
Sbjct: 148 EELGYIDGETLFGAPYDFRYGLAAPGHPSQVGTKFLNDLKSLIEKASISNGGKPVILVSH 207

Query: 78  SLGGLFVLHLLNRNPPSWRRHFIKHF 1
           SLGGLFV  LL+R+P SW + +IKHF
Sbjct: 208 SLGGLFVQQLLSRSPSSWYKKYIKHF 233


>ref|XP_002890730.1| lecithin:cholesterol acyltransferase family protein [Arabidopsis
           lyrata subsp. lyrata] gi|297336572|gb|EFH66989.1|
           lecithin:cholesterol acyltransferase family protein
           [Arabidopsis lyrata subsp. lyrata]
          Length = 432

 Score =  131 bits (329), Expect = 1e-28
 Identities = 59/83 (71%), Positives = 67/83 (80%)
 Frame = -1

Query: 249 GYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSHSLG 70
           GY   QT+ GAPYDFRYGLAA GHPS + +QFLQ+LK L+E+ S  N G+PVILLSHSLG
Sbjct: 152 GYVNDQTILGAPYDFRYGLAASGHPSRVASQFLQDLKQLVEKTSSENEGKPVILLSHSLG 211

Query: 69  GLFVLHLLNRNPPSWRRHFIKHF 1
           GLFVLH LNR  PSWRR +IKHF
Sbjct: 212 GLFVLHFLNRTTPSWRRKYIKHF 234


>ref|XP_006307463.1| hypothetical protein CARUB_v10009090mg [Capsella rubella]
           gi|482576174|gb|EOA40361.1| hypothetical protein
           CARUB_v10009090mg [Capsella rubella]
          Length = 456

 Score =  130 bits (327), Expect = 2e-28
 Identities = 59/83 (71%), Positives = 68/83 (81%)
 Frame = -1

Query: 249 GYTEGQTLFGAPYDFRYGLAAQGHPSHIGTQFLQNLKTLIEQASHSNNGQPVILLSHSLG 70
           GY   QTL GAPYDFRYGLAA GHPS + ++FLQ+LK+L+E+ S  NNG+PVILLSHSLG
Sbjct: 176 GYVNDQTLLGAPYDFRYGLAASGHPSRVASEFLQDLKSLVEKTSGENNGKPVILLSHSLG 235

Query: 69  GLFVLHLLNRNPPSWRRHFIKHF 1
           GLFVL  LNR  PSWRR +IKHF
Sbjct: 236 GLFVLQFLNRTTPSWRRKYIKHF 258