BLASTX nr result
ID: Cocculus22_contig00014169
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Cocculus22_contig00014169 (329 letters) Database: ./nr 38,876,450 sequences; 13,856,398,315 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AFW03483.1| pectin methylesterase 4 [Gossypium hirsutum] 185 7e-45 gb|AER38245.1| PME4 [Gossypium barbadense] 185 7e-45 gb|AFW03478.1| pectin methylesterase 4 [Gossypium barbadense] 184 1e-44 ref|XP_004146188.1| PREDICTED: pectinesterase/pectinesterase inh... 183 2e-44 ref|XP_006828588.1| hypothetical protein AMTR_s00129p00047530 [A... 182 4e-44 ref|XP_007012504.1| Pectinesterase/pectinesterase inhibitor PPE8... 182 6e-44 gb|AFW03477.1| pectin methylesterase 3 [Gossypium barbadense] 180 2e-43 gb|AER38246.1| PME3 [Gossypium barbadense] 180 2e-43 gb|AGR44473.1| pectin methylesterase 4, partial [Pyrus x bretsch... 179 3e-43 dbj|BAF42041.1| pectin methylesterase 4 [Pyrus communis] 179 3e-43 gb|AGJ83739.1| pectinesterase/pectinesterase inhibitor PPE8B-lik... 178 8e-43 ref|XP_006381541.1| Pectinesterase PPE8B precursor family protei... 177 1e-42 ref|XP_002516191.1| Pectinesterase PPE8B precursor, putative [Ri... 177 1e-42 gb|ABK92470.1| unknown [Populus trichocarpa] 177 1e-42 ref|XP_004242901.1| PREDICTED: pectinesterase/pectinesterase inh... 177 2e-42 gb|EYU28290.1| hypothetical protein MIMGU_mgv1a004392mg [Mimulus... 176 2e-42 ref|XP_007204326.1| hypothetical protein PRUPE_ppa004300mg [Prun... 175 5e-42 sp|Q43062.1|PME_PRUPE RecName: Full=Pectinesterase/pectinesteras... 175 5e-42 emb|CBI29628.3| unnamed protein product [Vitis vinifera] 175 5e-42 emb|CAN60612.1| hypothetical protein VITISV_003251 [Vitis vinifera] 175 5e-42 >gb|AFW03483.1| pectin methylesterase 4 [Gossypium hirsutum] Length = 525 Score = 185 bits (469), Expect = 7e-45 Identities = 86/109 (78%), Positives = 96/109 (88%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYREC ITGTVDFIFG+GAV+FQNC+ILA++ LP QKN+ITAQGRKDPNQ TGFSIQF Sbjct: 348 RQFYRECKITGTVDFIFGDGAVLFQNCQILAKQGLPSQKNTITAQGRKDPNQPTGFSIQF 407 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNISAD DLL NST TYLGRPWK YS+T+IMQSY+S IR +GWLEW Sbjct: 408 CNISADTDLLPSVNSTPTYLGRPWKLYSRTIIMQSYISDAIRPQGWLEW 456 >gb|AER38245.1| PME4 [Gossypium barbadense] Length = 525 Score = 185 bits (469), Expect = 7e-45 Identities = 86/109 (78%), Positives = 96/109 (88%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYREC ITGTVDFIFG+GAV+FQNC+ILA++ LP QKN+ITAQGRKDPNQ TGFSIQF Sbjct: 348 RQFYRECKITGTVDFIFGDGAVLFQNCQILAKQGLPSQKNTITAQGRKDPNQPTGFSIQF 407 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNISAD DLL NST TYLGRPWK YS+T+IMQSY+S IR +GWLEW Sbjct: 408 CNISADTDLLPSVNSTPTYLGRPWKLYSRTIIMQSYISDAIRPQGWLEW 456 >gb|AFW03478.1| pectin methylesterase 4 [Gossypium barbadense] Length = 525 Score = 184 bits (466), Expect = 1e-44 Identities = 85/109 (77%), Positives = 96/109 (88%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYREC ITGTVDFIFG+GAV+FQNC+ILA++ LP QKN+ITAQGRKDPNQ TGFSIQF Sbjct: 348 RQFYRECKITGTVDFIFGDGAVLFQNCQILAKQGLPSQKNTITAQGRKDPNQPTGFSIQF 407 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNISAD DLL NST TYLGRPW+ YS+T+IMQSY+S IR +GWLEW Sbjct: 408 CNISADTDLLPSVNSTPTYLGRPWRLYSRTIIMQSYISDAIRPQGWLEW 456 >ref|XP_004146188.1| PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis sativus] Length = 507 Score = 183 bits (465), Expect = 2e-44 Identities = 85/109 (77%), Positives = 94/109 (86%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYREC I+GTVDF+FG+ VVFQNC ILA+K LP QKN+ITAQGRKDPNQ TGFSIQF Sbjct: 329 RQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQF 388 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNISAD DL N+TATYLGRPWKEYS+T+IMQSY+S IR EGWLEW Sbjct: 389 CNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEW 437 >ref|XP_006828588.1| hypothetical protein AMTR_s00129p00047530 [Amborella trichopoda] gi|548833378|gb|ERM96004.1| hypothetical protein AMTR_s00129p00047530 [Amborella trichopoda] Length = 535 Score = 182 bits (462), Expect = 4e-44 Identities = 84/109 (77%), Positives = 90/109 (82%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYR+C I GTVDFIFGN A +FQNC IL RKPLP QKNSITA GRKDPNQ TGFSIQF Sbjct: 359 RQFYRDCDIFGTVDFIFGNAAAIFQNCRILPRKPLPNQKNSITAHGRKDPNQITGFSIQF 418 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNIS DP+L NST TYLGRPWK+YS+TVIMQSYMS VI +GWL W Sbjct: 419 CNISGDPELFPTKNSTETYLGRPWKQYSRTVIMQSYMSDVIHPQGWLAW 467 >ref|XP_007012504.1| Pectinesterase/pectinesterase inhibitor PPE8B [Theobroma cacao] gi|508782867|gb|EOY30123.1| Pectinesterase/pectinesterase inhibitor PPE8B [Theobroma cacao] Length = 541 Score = 182 bits (461), Expect = 6e-44 Identities = 85/109 (77%), Positives = 95/109 (87%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQF+REC ITGTVDFIFG+ VVFQ C+ILA++ LP QKN+ITAQGRKDPNQ TGFSIQF Sbjct: 364 RQFFRECKITGTVDFIFGDATVVFQYCQILAKQGLPNQKNTITAQGRKDPNQPTGFSIQF 423 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNISAD DLL NSTATYLGRPWK YS+T+IMQSY+S+ IR EGWLEW Sbjct: 424 CNISADTDLLPFVNSTATYLGRPWKLYSRTIIMQSYISNAIRPEGWLEW 472 >gb|AFW03477.1| pectin methylesterase 3 [Gossypium barbadense] Length = 514 Score = 180 bits (457), Expect = 2e-43 Identities = 82/109 (75%), Positives = 95/109 (87%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQF+REC ITGTVDFIFG+ +VVFQNC+ILA++ LP QKN+ITAQGRKDPNQ TGFSIQF Sbjct: 337 RQFFRECKITGTVDFIFGDASVVFQNCQILAKQGLPNQKNTITAQGRKDPNQPTGFSIQF 396 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNISAD DLL NST TYLGRPWK YS+T+IMQSY+ + +R +GWLEW Sbjct: 397 CNISADADLLPFVNSTPTYLGRPWKLYSRTIIMQSYIGNAVRPQGWLEW 445 >gb|AER38246.1| PME3 [Gossypium barbadense] Length = 514 Score = 180 bits (456), Expect = 2e-43 Identities = 82/109 (75%), Positives = 95/109 (87%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQF+REC ITGTVDFIFG+ +VVFQNC+ILA++ LP QKN+ITAQGRKDPNQ TGFSIQF Sbjct: 337 RQFFRECRITGTVDFIFGDASVVFQNCQILAKQGLPNQKNTITAQGRKDPNQPTGFSIQF 396 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNISAD DLL NST TYLGRPWK YS+T+IMQSY+ + +R +GWLEW Sbjct: 397 CNISADADLLPFVNSTPTYLGRPWKLYSRTIIMQSYIGNAVRPQGWLEW 445 >gb|AGR44473.1| pectin methylesterase 4, partial [Pyrus x bretschneideri] Length = 427 Score = 179 bits (455), Expect = 3e-43 Identities = 82/109 (75%), Positives = 95/109 (87%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYR+C I+GTVDFIFG+ VVFQNC+ILA+K LP QKNS+TAQGRKDPN+ TG SIQF Sbjct: 302 RQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSVTAQGRKDPNEPTGISIQF 361 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNI+AD DLL NST+TYLGRPWK YS+TVIMQS++S+ IR EGWLEW Sbjct: 362 CNITADADLLPFVNSTSTYLGRPWKLYSRTVIMQSFLSNAIRPEGWLEW 410 >dbj|BAF42041.1| pectin methylesterase 4 [Pyrus communis] Length = 509 Score = 179 bits (455), Expect = 3e-43 Identities = 82/109 (75%), Positives = 95/109 (87%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYR+C I+GTVDFIFG+ VVFQNC+ILA+K LP QKNS+TAQGRKDPN+ TG SIQF Sbjct: 332 RQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSVTAQGRKDPNEPTGISIQF 391 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNI+AD DLL NST+TYLGRPWK YS+TVIMQS++S+ IR EGWLEW Sbjct: 392 CNITADADLLPFVNSTSTYLGRPWKLYSRTVIMQSFLSNAIRPEGWLEW 440 >gb|AGJ83739.1| pectinesterase/pectinesterase inhibitor PPE8B-like protein, partial [Caragana korshinskii] Length = 480 Score = 178 bits (451), Expect = 8e-43 Identities = 81/109 (74%), Positives = 92/109 (84%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYREC I+GTVDFIFG+ +FQNC+ILA+K LP QKN ITA GRKDPN+ TGFSIQF Sbjct: 303 RQFYRECKISGTVDFIFGDATALFQNCQILAKKGLPNQKNPITAHGRKDPNEPTGFSIQF 362 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNISAD DLL + NST TYLGRPWK YS+T+ MQSY+S V+R EGWLEW Sbjct: 363 CNISADSDLLPVVNSTQTYLGRPWKPYSRTIFMQSYISDVLRPEGWLEW 411 >ref|XP_006381541.1| Pectinesterase PPE8B precursor family protein [Populus trichocarpa] gi|550336246|gb|ERP59338.1| Pectinesterase PPE8B precursor family protein [Populus trichocarpa] Length = 528 Score = 177 bits (449), Expect = 1e-42 Identities = 80/109 (73%), Positives = 93/109 (85%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYREC ++GTVDFIFG+ VFQNC+ILARK LP+QKN++TA GRKDPNQ TG+S QF Sbjct: 351 RQFYRECRVSGTVDFIFGDATAVFQNCQILARKGLPDQKNTVTAHGRKDPNQPTGYSFQF 410 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNISAD DLL NS+ TYLGRPWK +S+T+IMQSYMS+ IR EGWLEW Sbjct: 411 CNISADIDLLPYVNSSYTYLGRPWKNFSRTIIMQSYMSNAIRPEGWLEW 459 >ref|XP_002516191.1| Pectinesterase PPE8B precursor, putative [Ricinus communis] gi|223544677|gb|EEF46193.1| Pectinesterase PPE8B precursor, putative [Ricinus communis] Length = 299 Score = 177 bits (449), Expect = 1e-42 Identities = 81/109 (74%), Positives = 92/109 (84%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYREC I+GTVDFIFG+ VFQNC+ILA++ LP QKN+ITA GRKDPNQ TG+SIQF Sbjct: 122 RQFYRECRISGTVDFIFGDATAVFQNCQILAKRGLPNQKNTITAHGRKDPNQPTGYSIQF 181 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNISAD DLL NST TYLGRPWK +S+TV+MQSYMS +R EGWLEW Sbjct: 182 CNISADIDLLPYANSTYTYLGRPWKNFSRTVVMQSYMSDALRPEGWLEW 230 >gb|ABK92470.1| unknown [Populus trichocarpa] Length = 528 Score = 177 bits (449), Expect = 1e-42 Identities = 80/109 (73%), Positives = 93/109 (85%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYREC ++GTVDFIFG+ VFQNC+ILARK LP+QKN++TA GRKDPNQ TG+S QF Sbjct: 351 RQFYRECRVSGTVDFIFGDATAVFQNCQILARKGLPDQKNTVTAHGRKDPNQPTGYSFQF 410 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNISAD DLL NS+ TYLGRPWK +S+T+IMQSYMS+ IR EGWLEW Sbjct: 411 CNISADIDLLPYVNSSYTYLGRPWKNFSRTIIMQSYMSNAIRPEGWLEW 459 >ref|XP_004242901.1| PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Solanum lycopersicum] Length = 541 Score = 177 bits (448), Expect = 2e-42 Identities = 81/109 (74%), Positives = 94/109 (86%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYR+C ITGTVDFIFG+G +FQNC+ILARK L +QKN+ITAQGRK+ + TGF+IQF Sbjct: 364 RQFYRDCTITGTVDFIFGDGTAIFQNCQILARKGLSQQKNTITAQGRKEIAETTGFTIQF 423 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNIS +PDLL NST TYLGRPWK YS+TVIMQSYMS+VIR +GWLEW Sbjct: 424 CNISGEPDLLTSLNSTYTYLGRPWKTYSRTVIMQSYMSNVIRPQGWLEW 472 >gb|EYU28290.1| hypothetical protein MIMGU_mgv1a004392mg [Mimulus guttatus] Length = 530 Score = 176 bits (447), Expect = 2e-42 Identities = 85/109 (77%), Positives = 94/109 (86%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYRECVITGTVDFIFG+GAVVFQNC I ARK LP+QKN+ITAQGRK+P QNTGFSIQF Sbjct: 354 RQFYRECVITGTVDFIFGDGAVVFQNCNIQARKGLPDQKNTITAQGRKEPVQNTGFSIQF 413 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNIS D+L N+T TYLGRPWK YS+TVIMQSY+S +R EGWLEW Sbjct: 414 CNISVANDVL---NTTQTYLGRPWKLYSRTVIMQSYISGSVRPEGWLEW 459 >ref|XP_007204326.1| hypothetical protein PRUPE_ppa004300mg [Prunus persica] gi|462399857|gb|EMJ05525.1| hypothetical protein PRUPE_ppa004300mg [Prunus persica] Length = 517 Score = 175 bits (444), Expect = 5e-42 Identities = 84/110 (76%), Positives = 95/110 (86%), Gaps = 1/110 (0%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYR+C I+GTVDFIFG+ VVFQNC+ILA+K LP QKNSITAQGRKDPN+ TG SIQF Sbjct: 339 RQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQF 398 Query: 149 CNISADPDL-LALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNI+AD DL A NST TYLGRPWK YS+TVIMQS++S+VIR EGWLEW Sbjct: 399 CNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEW 448 >sp|Q43062.1|PME_PRUPE RecName: Full=Pectinesterase/pectinesterase inhibitor PPE8B; Includes: RecName: Full=Pectinesterase inhibitor PPE8B; AltName: Full=Pectin methylesterase inhibitor PPE8B; Includes: RecName: Full=Pectinesterase PPE8B; Short=PE PPE8B; AltName: Full=Pectin methylesterase PPE8B; Flags: Precursor gi|1213629|emb|CAA65237.1| pectinesterase [Prunus persica] Length = 522 Score = 175 bits (444), Expect = 5e-42 Identities = 84/110 (76%), Positives = 95/110 (86%), Gaps = 1/110 (0%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYR+C I+GTVDFIFG+ VVFQNC+ILA+K LP QKNSITAQGRKDPN+ TG SIQF Sbjct: 344 RQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQF 403 Query: 149 CNISADPDL-LALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 CNI+AD DL A NST TYLGRPWK YS+TVIMQS++S+VIR EGWLEW Sbjct: 404 CNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEW 453 >emb|CBI29628.3| unnamed protein product [Vitis vinifera] Length = 527 Score = 175 bits (444), Expect = 5e-42 Identities = 82/109 (75%), Positives = 93/109 (85%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYREC I+GTVDFIFG+ VVFQNC+IL +K LP QKN+ITAQGRKDP Q TGFSIQF Sbjct: 350 RQFYRECRISGTVDFIFGDATVVFQNCQILVKKGLPNQKNTITAQGRKDPAQPTGFSIQF 409 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 NISAD DLLA NST +YLGRPWK+YS+T+IM+SY+S IR EGWLEW Sbjct: 410 SNISADSDLLASVNSTLSYLGRPWKQYSRTIIMKSYISDAIRPEGWLEW 458 >emb|CAN60612.1| hypothetical protein VITISV_003251 [Vitis vinifera] Length = 534 Score = 175 bits (444), Expect = 5e-42 Identities = 82/109 (75%), Positives = 93/109 (85%) Frame = -1 Query: 329 RQFYRECVITGTVDFIFGNGAVVFQNCEILARKPLPEQKNSITAQGRKDPNQNTGFSIQF 150 RQFYREC I+GTVDFIFG+ VVFQNC+IL +K LP QKN+ITAQGRKDP Q TGFSIQF Sbjct: 354 RQFYRECRISGTVDFIFGDATVVFQNCQILVKKGLPNQKNTITAQGRKDPAQPTGFSIQF 413 Query: 149 CNISADPDLLALPNSTATYLGRPWKEYSKTVIMQSYMSSVIRSEGWLEW 3 NISAD DLLA NST +YLGRPWK+YS+T+IM+SY+S IR EGWLEW Sbjct: 414 SNISADSDLLASVNSTLSYLGRPWKQYSRTIIMKSYISDAIRPEGWLEW 462