BLASTX nr result
ID: Cocculus22_contig00011846
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cocculus22_contig00011846
(842 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AHF65153.1| stylopine synthase, partial [synthetic construct] 224 3e-56
gb|ADB89214.1| stylopine synthase [Papaver somniferum] 224 3e-56
dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta] 223 7e-56
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g... 219 1e-54
sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Sh... 218 2e-54
gb|ADB89213.1| cheilanthifoline synthase [Papaver somniferum] 214 2e-53
sp|B1NF20.1|C719E_ARGME RecName: Full=Cheilanthifoline synthase;... 214 4e-53
sp|B5UAQ8.1|C7195_ESCCA RecName: Full=Cheilanthifoline synthase;... 213 7e-53
gb|AGL76711.1| (S)-canadine synthase, partial [Coptis chinensis] 213 9e-53
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt... 211 2e-52
gb|AGC29954.1| CYP719A24 [Podophyllum peltatum] 211 3e-52
gb|AGC29953.1| CYP719A23 [Sinopodophyllum hexandrum] 209 8e-52
dbj|BAG75115.1| cytochrome P450 [Eschscholzia californica] 209 8e-52
dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta] 209 8e-52
sp|B1NF18.1|C719B_PAPSO RecName: Full=Salutaridine synthase; Alt... 209 1e-51
gb|AFB74615.1| cytochrome P450 [Papaver somniferum] 207 3e-51
gb|AFI57927.1| cytochrome P450, partial [Papaver somniferum] 207 5e-51
dbj|BAG75114.1| cytochrome P450 [Eschscholzia californica] 205 2e-50
dbj|BAG75116.1| cytochrome P450 [Eschscholzia californica] 204 3e-50
sp|Q50LH4.1|C7193_ESCCA RecName: Full=(S)-stylopine synthase 2; ... 204 3e-50
>gb|AHF65153.1| stylopine synthase, partial [synthetic construct]
Length = 511
Score = 224 bits (571), Expect = 3e-56
Identities = 117/280 (41%), Positives = 179/280 (63%), Gaps = 1/280 (0%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG + DD + + ++ AI E I++ L + F + +Y+P K +++E QR
Sbjct: 209 RLIFGQDFD-DDKYVDDMHHAIEELIRVSGYAQLAEAFYYAKYLPSHKKAVRDVEEANQR 267
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
VK+L+ ++S + P+ N YL FL SQ + E+++I +FE ++ VDSTS T AWALAF+
Sbjct: 268 VKKLVRPFLSLNPPT--NTYLHFLNSQKYDEEVIIFAIFEAYLLGVDSTSLTTAWALAFL 325
Query: 362 IHHEDIQQRVLTQVMNKLGGTR-RMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRA 538
I +Q ++ ++ N +V VEDL +++Y+ AV+KET+R +PI P +P +A
Sbjct: 326 IREPVVQDKLYQELKNLTAKNDCEIVKVEDLN-KLQYLQAVLKETMRMKPIAPLAIPHKA 384
Query: 539 TRDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERS 718
RD+ LKG + +GTQV+VN+YA+ H+E+VWKEP KFMPERFL + +G+ ME+S
Sbjct: 385 CRDTSLKGNKIDQGTQVMVNIYALHHNEKVWKEPFKFMPERFLQTQDVVNGKG---MEQS 441
Query: 719 FISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEG 838
+ F AG R C GMEL K + + +LANLVN+F+W +G
Sbjct: 442 LLPFSAGMRICAGMELGKLQFSFSLANLVNAFEWSCVSDG 481
>gb|ADB89214.1| stylopine synthase [Papaver somniferum]
Length = 494
Score = 224 bits (571), Expect = 3e-56
Identities = 117/280 (41%), Positives = 179/280 (63%), Gaps = 1/280 (0%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG + DD + + ++ AI E I++ L + F + +Y+P K +++E QR
Sbjct: 195 RLIFGQDFD-DDKYVDDMHHAIEELIRVSGYAQLAEAFYYAKYLPSHKKAVRDVEEANQR 253
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
VK+L+ ++S + P+ N YL FL SQ + E+++I +FE ++ VDSTS T AWALAF+
Sbjct: 254 VKKLVRPFLSLNPPT--NTYLHFLNSQKYDEEVIIFAIFEAYLLGVDSTSLTTAWALAFL 311
Query: 362 IHHEDIQQRVLTQVMNKLGGTR-RMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRA 538
I +Q ++ ++ N +V VEDL +++Y+ AV+KET+R +PI P +P +A
Sbjct: 312 IREPVVQDKLYQELKNLTAKNDCEIVKVEDLN-KLQYLQAVLKETMRMKPIAPLAIPHKA 370
Query: 539 TRDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERS 718
RD+ LKG + +GTQV+VN+YA+ H+E+VWKEP KFMPERFL + +G+ ME+S
Sbjct: 371 CRDTSLKGNKIDQGTQVMVNIYALHHNEKVWKEPFKFMPERFLQTQDVVNGKG---MEQS 427
Query: 719 FISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEG 838
+ F AG R C GMEL K + + +LANLVN+F+W +G
Sbjct: 428 LLPFSAGMRICAGMELGKLQFSFSLANLVNAFEWSCVSDG 467
>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 491
Score = 223 bits (568), Expect = 7e-56
Identities = 119/284 (41%), Positives = 179/284 (63%), Gaps = 4/284 (1%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FGP +D F E + I + ++L L + F + +Y+P K +E K R
Sbjct: 192 RLIFGPTFD-NDKFVESVLYEIEDVLRLSGYARLTEAFYYAKYLPSQKKVAREAYLAKYR 250
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
++EL+ +S++ P++S YL FL SQ ++++I+I FELF+ VDSTS+T WALA++
Sbjct: 251 IEELMRPLLSSNPPTNS--YLYFLQSQNYSKEIIIFCTFELFLLGVDSTSSTATWALAYL 308
Query: 362 IHHEDIQQRVLTQVMNKLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRAT 541
IH + +Q+++ + +G +V +EDL ++++Y+ AVVKET+R +PI P +P +
Sbjct: 309 IHEQAVQEKLNEDIRMTIGDVD-LVKIEDL-SKLKYLQAVVKETMRMKPIAPLAIPHKTA 366
Query: 542 RDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVT----RM 709
D+ L G V GTQ++VNLYA+ H+E +W EP KFMPERFL GE+V+ M
Sbjct: 367 NDTTLMGTKVARGTQIMVNLYALHHNEDIWPEPYKFMPERFL------QGENVSANNKAM 420
Query: 710 ERSFISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEGQ 841
E+SF+ FGAG R C GM+L K +IA LANLVN+F+W EG+
Sbjct: 421 EQSFLPFGAGMRICAGMDLGKLQIAFALANLVNAFKWSCVDEGK 464
>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
Length = 492
Score = 219 bits (558), Expect = 1e-54
Identities = 115/280 (41%), Positives = 180/280 (64%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG ++ DD F E ++ I + I++ L + F + +Y+P K +E +K R
Sbjct: 193 RLIFG-QTFDDDKFVESMHFEIDDIIRISGYARLAEAFYYAKYLPSHKKAEREAYLVKCR 251
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
V++L+S +S++ P++S YL FLLSQ F+E+++I +FEL++ VDSTS+T WALA++
Sbjct: 252 VEDLVSPLLSSNPPTNS--YLYFLLSQRFSEEVIIFCIFELYLLGVDSTSSTTTWALAYL 309
Query: 362 IHHEDIQQRVLTQVMNKLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRAT 541
I + IQ+++ LG +V +ED+ ++++Y+ AVVKET+R +PI P +P +
Sbjct: 310 IREQAIQEKLYQDTRMTLGDVD-LVKIEDV-SKLKYLQAVVKETMRMKPIAPLAIPHKTA 367
Query: 542 RDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERSF 721
+D+ L G V +GT+++VNLYA+ H++ +W P KFMPERFL G G + ME+SF
Sbjct: 368 KDTTLMGSKVAKGTRIMVNLYALHHNQNIWTNPYKFMPERFL-QGEDGSASN-KAMEQSF 425
Query: 722 ISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEGQ 841
+ F AG R C GM+L K + A LANLVN+F+W EG+
Sbjct: 426 LPFSAGMRICAGMDLGKLQFAFALANLVNAFKWSCVEEGK 465
>sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Short=STS; AltName:
Full=Cytochrome P450 719A13 gi|148841092|gb|ABR14721.1|
stylopine synthase [Argemone mexicana]
Length = 504
Score = 218 bits (555), Expect = 2e-54
Identities = 112/280 (40%), Positives = 174/280 (62%), Gaps = 1/280 (0%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL +G + D+ + E ++ I E I++ L + F + +Y+P K +E+ QR
Sbjct: 204 RLIYGQDFDNDE-YVEEMHHTIEELIRVSGYARLAEAFYYAKYLPSHKKAVREVLQANQR 262
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
V+ L+ +S + P+ N YL FL SQ + ++++I +FE ++ VDSTS+T AWALA++
Sbjct: 263 VQNLVRPLLSLNSPT--NTYLHFLRSQNYEDEVIIFAIFEAYLLGVDSTSSTTAWALAYL 320
Query: 362 IHHEDIQQRVLTQVMNKLGGT-RRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRA 538
I ++Q+++ ++ N R+MV EDL +++Y+ AVVKET+R +PI P +P +A
Sbjct: 321 IREPNVQEKLYEELKNFTNDNDRKMVKFEDLN-KLQYLQAVVKETMRMKPIAPLAIPHKA 379
Query: 539 TRDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERS 718
R++ L G V +GT+V+VN+YA+ H++ VWKEP KF PERFL DG D ME+S
Sbjct: 380 CRETSLMGRKVNQGTRVMVNIYALHHNQNVWKEPYKFNPERFLQKNQ--DGVDGKAMEQS 437
Query: 719 FISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEG 838
+ F AG R C GMEL K + + LANLVN+F+W +G
Sbjct: 438 LLPFSAGMRICAGMELGKLQFSFALANLVNAFKWSCVSDG 477
>gb|ADB89213.1| cheilanthifoline synthase [Papaver somniferum]
Length = 492
Score = 214 bits (546), Expect = 2e-53
Identities = 113/281 (40%), Positives = 177/281 (62%), Gaps = 1/281 (0%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG + +D+ F G++ A+ E + + L D F+F +P K + + ++K R
Sbjct: 190 RLIFG-QDFVDEDFVVGMHQALDELVSISGYASLADAFKFCENLPSHKKTVRAVHAIKIR 248
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
LI +I ++ P+ N YL FLLSQ F+ED++I+ + E++ VDST++T WAL F+
Sbjct: 249 FDNLIRPHIVSNPPT--NTYLHFLLSQDFSEDVIISAILEVYDLGVDSTASTTVWALTFL 306
Query: 362 IHHEDIQQRVLTQVMNKLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRAT 541
+ + IQ+++ ++ N GG ++ V VEDL ++ Y+ AV+KET+R +PI P +P +A+
Sbjct: 307 VREQKIQEKLYREINNVTGG-KKPVKVEDLN-KLPYLQAVMKETMRMKPIAPMAIPHKAS 364
Query: 542 RDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLM-SGSSGDGEDVTRMERS 718
+D+ L G + +G V+VNLYA+ H+ V+ EP KFMPERFL + S G D+ +ME S
Sbjct: 365 KDTSLMGKKINKGAVVMVNLYAIHHNPAVFPEPYKFMPERFLKDANSDGSLGDIKKMESS 424
Query: 719 FISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEGQ 841
++F AG R C GMEL K ++A LA+LVN F+W EG+
Sbjct: 425 LLAFSAGMRICAGMELGKLQLAFGLASLVNEFKWDCFAEGK 465
>sp|B1NF20.1|C719E_ARGME RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
Full=Cytochrome P450 719A14 gi|148841094|gb|ABR14722.1|
cheilanthifoline synthase [Argemone mexicana]
Length = 494
Score = 214 bits (544), Expect = 4e-53
Identities = 108/281 (38%), Positives = 181/281 (64%), Gaps = 1/281 (0%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG + D+ G++ A+ + +++ L D F+F +P K +E+ +K+R
Sbjct: 192 RLIFG-QDFQDEKLVVGMHHALDDLVRISGYASLADAFKFCENLPSHKKSIREVHEVKKR 250
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
V+ LI +I ++ P+ N YL FL +Q F EDI+I+ + E++ VDST++T WAL F+
Sbjct: 251 VENLIRPHIVSNPPT--NTYLYFLKTQDFNEDIIISAILEVYDLGVDSTASTTVWALTFL 308
Query: 362 IHHEDIQQRVLTQVMNKLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRAT 541
+ ++IQ+++ +++N GG +R V VED+ +M Y+ AV+KET+R +PI P +P + +
Sbjct: 309 VREQEIQEKLYREIVNVTGG-KRSVKVEDVN-KMPYLQAVMKETMRMKPIAPMAIPHKTS 366
Query: 542 RDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDG-EDVTRMERS 718
+D+ L G + +G+ ++VNLYA+ H+ +V+ EP KFMPERFL +S + ++ ME S
Sbjct: 367 KDTSLMGKKINKGSVIMVNLYAIHHNPKVFPEPYKFMPERFLKDVNSDESLGNIKTMESS 426
Query: 719 FISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEGQ 841
++F AG R C GMEL K ++A LA+LV+ F+W +V+G+
Sbjct: 427 LLAFSAGMRICAGMELGKLQLAFGLASLVHEFKWSCSVDGK 467
>sp|B5UAQ8.1|C7195_ESCCA RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
Full=Cytochrome P450 719A5 gi|209571368|dbj|BAG75113.1|
cheilanthifoline synthase [Eschscholzia californica]
Length = 490
Score = 213 bits (542), Expect = 7e-53
Identities = 107/280 (38%), Positives = 178/280 (63%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG +S D+ F +G++ A+ + +++ L D F+F +P K +E+ + +R
Sbjct: 189 RLIFG-QSFNDENFVKGVHLALDDLVRISGYASLADAFKFCENLPSHKKSIREVHEVNER 247
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
V L+ Y+ + P+ N YL FL SQ F+++++I+ + E++ VDST++T WAL F+
Sbjct: 248 VVNLVKPYLVKNPPT--NTYLYFLNSQKFSDEVIISAVLEVYDLGVDSTASTAVWALTFL 305
Query: 362 IHHEDIQQRVLTQVMNKLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRAT 541
+ +Q+++ ++++ GG R V VED+ +++ Y+ AV+KET+R +PI P +P + +
Sbjct: 306 VREPRVQEKLYKEIIDLTGG-ERSVKVEDV-SKLPYLQAVMKETMRMKPIAPMAIPHKTS 363
Query: 542 RDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERSF 721
RD+ L G V +GT ++VNLYA+ H+ +V+ EP KF+PERFL S G D+ ME+S
Sbjct: 364 RDTSLMGKKVNKGTSIMVNLYAIHHNPKVFPEPYKFIPERFLQGQESKYG-DIKEMEQSL 422
Query: 722 ISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEGQ 841
+ F AG R C GMEL K + +LA+LV +F+W AV+G+
Sbjct: 423 LPFSAGMRICAGMELGKLQYGFSLASLVEAFKWTCAVDGK 462
>gb|AGL76711.1| (S)-canadine synthase, partial [Coptis chinensis]
Length = 491
Score = 213 bits (541), Expect = 9e-53
Identities = 111/280 (39%), Positives = 179/280 (63%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG ++ D+ F E ++ I + I++ L + F + +Y+P K +E +K R
Sbjct: 192 RLIFG-QNFDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCR 250
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
V+EL+ +S++ P++S YL FLLSQ F E+++I +FEL++ VDSTS+T WALA++
Sbjct: 251 VQELVRPLLSSNPPTNS--YLYFLLSQNFEEEVIIFCIFELYLLGVDSTSSTTTWALAYL 308
Query: 362 IHHEDIQQRVLTQVMNKLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRAT 541
I + Q+++ + LG +V +ED+ +++Y+ VVKET+R +PI P +P +
Sbjct: 309 IREQGAQEKLYQDIRMTLGDVD-LVKIEDVN-KLKYLQGVVKETMRMKPIAPLAIPHKTA 366
Query: 542 RDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERSF 721
+++ L G V +GT+++VNLYA+ H++ +W +P KFMPERFL G++G + ME+SF
Sbjct: 367 KETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPYKFMPERFL-EGATGTAYN-KAMEQSF 424
Query: 722 ISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEGQ 841
+ F AG R C GM+L K + A LANLVN+F+W EG+
Sbjct: 425 LPFSAGMRICAGMDLGKLQFAFALANLVNAFKWSCVEEGK 464
>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
Full=(S)-tetrahydrocolumbamine oxidase
(methylenedioxy-bridge-forming); AltName: Full=Cytochrome
P450 719A1 gi|15822820|dbj|BAB68769.1| methylenedioxy
bridge-forming enzyme [Coptis japonica]
Length = 491
Score = 211 bits (538), Expect = 2e-52
Identities = 111/280 (39%), Positives = 177/280 (63%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG ++ D+ F E ++ I + I++ L + F + +Y+P K +E +K R
Sbjct: 192 RLIFG-QAFDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCR 250
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
V+EL+ +S+ P++S YL FLLSQ F E+++I +FEL++ VDSTS+T WALA++
Sbjct: 251 VEELVRPLLSSKPPTNS--YLYFLLSQNFEEEVIIFCIFELYLLGVDSTSSTTTWALAYL 308
Query: 362 IHHEDIQQRVLTQVMNKLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRAT 541
I + Q+++ + LG +V +ED+ +++Y+ VVKET+R +PI P +P +
Sbjct: 309 IREQGAQEKLYQDIRMTLGDVD-LVKIEDVN-KLKYLQGVVKETMRMKPIAPLAIPHKTA 366
Query: 542 RDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERSF 721
+++ L G V +GT+++VNLYA+ H++ +W +P KFMPERFL G +G + ME+SF
Sbjct: 367 KETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPYKFMPERFL-EGETGTAYN-KAMEQSF 424
Query: 722 ISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEGQ 841
+ F AG R C GM+L K + A LANLVN+F+W EG+
Sbjct: 425 LPFSAGMRICAGMDLGKLQFAFALANLVNAFKWSCVEEGK 464
>gb|AGC29954.1| CYP719A24 [Podophyllum peltatum]
Length = 489
Score = 211 bits (537), Expect = 3e-52
Identities = 113/280 (40%), Positives = 173/280 (61%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG ++ +D + + ++ + + + L F F +Y+P K KE K++
Sbjct: 191 RLIFG-DTFDNDPYNDSMHEVVQDLNRFGGIALLEQAFSFAKYLPSYKRGVKEFHIHKRK 249
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
+ +L+ +++ P S N YL FL SQ ++E+I+IA +FEL++ DS+++T WALAF
Sbjct: 250 IDDLVRPVVASSNPPS-NSYLGFLQSQNYSEEIIIACIFELYLLXXDSSASTATWALAFX 308
Query: 362 IHHEDIQQRVLTQVMNKLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRAT 541
I + +Q+++ + +G +V EDL ++M Y+ AVVKET+R +PI P +P +
Sbjct: 309 IRDQQVQEKLYQDIKRVIGDEVGLVKAEDL-SKMHYLQAVVKETMRMKPIAPLAIPHKTA 367
Query: 542 RDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERSF 721
D+ L G V +GT V+VNLYA+ HDE VW +P FMPERFL DG+ VT E++F
Sbjct: 368 IDTSLMGTKVPKGTCVMVNLYALHHDESVWAKPYTFMPERFL---QREDGKSVT--EQAF 422
Query: 722 ISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEGQ 841
+ FGAG R C GME+ K + +L LANLVN+F+W SA EG+
Sbjct: 423 LPFGAGMRICGGMEVGKLQFSLALANLVNAFKWTSAAEGK 462
>gb|AGC29953.1| CYP719A23 [Sinopodophyllum hexandrum]
Length = 489
Score = 209 bits (533), Expect = 8e-52
Identities = 111/280 (39%), Positives = 176/280 (62%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG ++ +D + + ++ + + + L F F +++P K KE K++
Sbjct: 191 RLIFG-DTFDNDPYNDSMHEVVQDLNRFGGIALLEQAFSFAKHLPSYKRGVKEFHIHKRK 249
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
+ +L+ +++ P S N YL FL SQ ++E+I+IA +FEL++ ++DS+++T WALAF+
Sbjct: 250 IDDLVRPVVASANPPS-NSYLGFLQSQNYSEEIIIACIFELYLLAMDSSASTATWALAFM 308
Query: 362 IHHEDIQQRVLTQVMNKLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRAT 541
I + +Q+++ + +G +V EDL ++M Y+ AVVKET+R +PI P +P +
Sbjct: 309 IRDQQVQEKLYQDIKRVIGDGVDLVKAEDL-SKMHYLQAVVKETMRMKPIAPLAIPHKTA 367
Query: 542 RDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERSF 721
D+ + G V +GT V+VNLYA+ HDE VW +P FMPERFL DG+ VT E++F
Sbjct: 368 IDTTVMGTKVPKGTCVMVNLYALHHDESVWAKPYTFMPERFL---QGEDGKSVT--EQAF 422
Query: 722 ISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEGQ 841
+ FGAG R C GME+ K + +L LANLVN+F+W SA EG+
Sbjct: 423 LPFGAGMRICGGMEVGKLQFSLALANLVNAFKWTSAAEGK 462
>dbj|BAG75115.1| cytochrome P450 [Eschscholzia californica]
Length = 494
Score = 209 bits (533), Expect = 8e-52
Identities = 109/280 (38%), Positives = 171/280 (61%), Gaps = 1/280 (0%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FGP + DD + E ++ I E I++ L + F + +Y+P + + E KQ
Sbjct: 196 RLVFGPNFN-DDQYVEDMHNTIDELIQMSSYAQLAEAFYYAKYLPSHRKAVRHFEQTKQI 254
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
VK+LI YIS P N YL FL SQ + E++VI +FE ++ VDSTS+T WALAF+
Sbjct: 255 VKKLIRPYISVSPPP--NTYLHFLQSQNYDEEVVIFAIFEAYLLGVDSTSSTTTWALAFL 312
Query: 362 IHHEDIQQRVLTQVMN-KLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRA 538
I ++Q+++ ++ N R ++ VED+ +++Y+ AV KET+R +P+ P +P +A
Sbjct: 313 IREPNVQEKLYQELKNFAEQNDREIMKVEDI-NKLQYLQAVTKETMRMKPVAPLAIPHKA 371
Query: 539 TRDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERS 718
R++ L GM + +GT+V+VN++++ H+E V+ +P FMPERFL D ++V ME+S
Sbjct: 372 CRETSLMGMPIDKGTKVMVNIHSLHHNENVFSDPFTFMPERFL----KVDNQNVKAMEQS 427
Query: 719 FISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEG 838
++ F AG R C G+EL K LANLV +F+W +G
Sbjct: 428 YLPFSAGMRICAGIELGKLHFGFALANLVYAFKWSCVADG 467
>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 508
Score = 209 bits (533), Expect = 8e-52
Identities = 114/282 (40%), Positives = 174/282 (61%), Gaps = 2/282 (0%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FGP S D F E + I + I+ L + F + +Y+P + +E + K R
Sbjct: 208 RLIFGP-SFNDSKFIESMLYEIEDFIRTSSYTSLTNSFYYAKYLPSQRKATREAKLGKYR 266
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
++EL+ +S+ P+ N YL FLLSQ +E++ I +FELF+ VDS S+T WALA++
Sbjct: 267 IEELVRPLLSSKPPT--NAYLYFLLSQNLSEEVTIFCIFELFLLGVDSASSTTTWALAYM 324
Query: 362 IHHEDIQQRVLTQVMNKLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRAT 541
I ++ Q+++ + +G +V +ED+ +++Y+ +VVKET+R +PI P LP A
Sbjct: 325 IREQEAQEKLYQDIRMTIGDVD-LVKIEDVN-KLKYLQSVVKETMRMKPIAPLALPHNAG 382
Query: 542 RDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTR--MER 715
+D+ L G + +GT+V+VNLYA+ H E VW EP+KFMPER+L G+ VT ME+
Sbjct: 383 KDTTLMGTKIVKGTRVMVNLYALHHYEDVWLEPSKFMPERYL----QGEDGSVTHKAMEQ 438
Query: 716 SFISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEGQ 841
S + FGAG R C GM+L K ++A LANLVN+F+W +G+
Sbjct: 439 SLLPFGAGMRICAGMDLGKLQVAFVLANLVNAFKWSCVEDGK 480
>sp|B1NF18.1|C719B_PAPSO RecName: Full=Salutaridine synthase; AltName: Full=Cytochrome P450
719B1 gi|148841090|gb|ABR14720.1| salutaridine synthase
[Papaver somniferum]
Length = 505
Score = 209 bits (531), Expect = 1e-51
Identities = 112/281 (39%), Positives = 176/281 (62%), Gaps = 1/281 (0%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
R+ FG + S +DL G+ A+ E +++ L D F+ +Y+P + +++ + + R
Sbjct: 199 RVIFGHDFSNEDLVI-GVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDR 257
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
V LI +I + P++S +L FL SQ ++++I+ + + E+F VDST+ T WAL+F+
Sbjct: 258 VYNLIQPHIVPNLPANS--FLYFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFL 315
Query: 362 IHHEDIQQRVLTQVMNKLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRAT 541
+ ++IQ+++ ++ N+ GG +R V V DLK E+ Y+ AV+KET+R +PI P +P A
Sbjct: 316 VGEQEIQEKLYREINNRTGG-QRPVKVVDLK-ELPYLQAVMKETLRMKPIAPLAVPHVAA 373
Query: 542 RDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMS-GSSGDGEDVTRMERS 718
+D+ KG + +GT+V+VNLYA+ HD V+ P KFMPERFL S G D+ ME S
Sbjct: 374 KDTTFKGRRIVKGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESS 433
Query: 719 FISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEGQ 841
I FGAG R C G+ELAK +A LA++VN F+W EG+
Sbjct: 434 LIPFGAGMRICGGVELAKQMVAFALASMVNEFKWDCVSEGK 474
>gb|AFB74615.1| cytochrome P450 [Papaver somniferum]
Length = 486
Score = 207 bits (528), Expect = 3e-51
Identities = 110/280 (39%), Positives = 171/280 (61%), Gaps = 1/280 (0%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL +G + DD + E ++ I I++ L +VF + +Y+PG K E K+R
Sbjct: 191 RLIYGQDFD-DDKYVEDMHDVIEFLIRISGYAQLAEVFYYAKYLPGHKRAVTGAEEAKRR 249
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
V L+ ++ ++ ++N YL FL SQ + E+++I +FE ++ VDSTS+T AWALAF+
Sbjct: 250 VIALVRPFLQSNP--ATNTYLHFLKSQLYPEEVIIFAIFEAYLLGVDSTSSTTAWALAFL 307
Query: 362 IHHEDIQQRVLTQVMN-KLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRA 538
I +Q+++ ++ N R M+ VED+ ++ Y+ AVVKET+R +PI P +P +A
Sbjct: 308 IREPSVQEKLYQELKNFTANNNRTMLKVEDVN-KLPYLQAVVKETMRMKPIAPLAIPHKA 366
Query: 539 TRDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERS 718
+D+ L G V +GT+V+VN++A+ H E+VWKEP KF+PERFL ME+S
Sbjct: 367 CKDTSLMGKKVDKGTKVMVNIHALHHTEKVWKEPYKFIPERFLQKHDKA-------MEQS 419
Query: 719 FISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEG 838
+ F AG R C GMEL K + + +LANLVN+F+W +G
Sbjct: 420 LLPFSAGMRICAGMELGKLQFSFSLANLVNAFKWSCVSDG 459
>gb|AFI57927.1| cytochrome P450, partial [Papaver somniferum]
Length = 488
Score = 207 bits (526), Expect = 5e-51
Identities = 110/280 (39%), Positives = 170/280 (60%), Gaps = 1/280 (0%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL +G + DD + E ++ I I++ L +VF + +Y+P K E K+R
Sbjct: 193 RLIYGQDFD-DDKYVEDMHDVIEFLIRISGYAQLAEVFYYAKYLPSHKRAVTGAEEAKRR 251
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
V L+ ++ ++ ++N YL FL SQ + E+++I +FE ++ VDSTS+T AWALAF+
Sbjct: 252 VIALVRPFLQSNP--ATNTYLHFLKSQLYPEEVIIFAIFEAYLLGVDSTSSTTAWALAFL 309
Query: 362 IHHEDIQQRVLTQVMN-KLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRA 538
I +Q+++ ++ N R M+ VED+ ++ Y+ AVVKET+R +PI P +P +A
Sbjct: 310 IREPSVQEKLYQELKNFTANNNRTMLKVEDVN-KLPYLQAVVKETMRMKPIAPLAIPHKA 368
Query: 539 TRDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERS 718
+D+ L G V +GT+V+VN++A+ H E+VWKEP KFMPERFL ME+S
Sbjct: 369 CKDTSLMGKKVDKGTKVMVNIHALHHTEKVWKEPYKFMPERFLQKHDKA-------MEQS 421
Query: 719 FISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEG 838
+ F AG R C GMEL K + + +LANLVN+F+W +G
Sbjct: 422 LLPFSAGMRICAGMELGKLQFSFSLANLVNAFKWSCVSDG 461
>dbj|BAG75114.1| cytochrome P450 [Eschscholzia californica]
Length = 495
Score = 205 bits (522), Expect = 2e-50
Identities = 108/280 (38%), Positives = 172/280 (61%), Gaps = 1/280 (0%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG + +D + E ++ I E I++ L + F + RY+P K KQ
Sbjct: 196 RLIFGQDFD-EDTYVEKLHHTIDELIRMSGYAQLAEAFYYARYLPNHKKAVNHAAKTKQI 254
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
V L+ ++S + P++S YL FL SQ + E+++I ++FE+++ VDSTS+T AWALA++
Sbjct: 255 VTHLMRPFLSLNPPTNS--YLHFLQSQNYDEELIIFSIFEVYLLGVDSTSSTTAWALAYL 312
Query: 362 IHHEDIQQRVLTQVMN-KLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRA 538
+ ++Q+ + ++ N R+++ VED+ +++Y+ AV KET+R +PI P +P +A
Sbjct: 313 VREPNVQETLYQELDNFAKQNDRKILKVEDIN-KLQYLQAVTKETMRMKPIAPLAIPHKA 371
Query: 539 TRDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERS 718
+RD+ L G V +GT+V+VNL+A+ H+E V+ +P KFMPERFL S G ME+S
Sbjct: 372 SRDTTLMGKKVEKGTKVMVNLHALHHNENVFNDPYKFMPERFL---KSYQGAKAKAMEQS 428
Query: 719 FISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEG 838
++ F AG R C GME+ K + LANL +F+W AV G
Sbjct: 429 YLPFSAGMRICGGMEVGKLQFGFALANLAYAFKWSCAVNG 468
>dbj|BAG75116.1| cytochrome P450 [Eschscholzia californica]
Length = 495
Score = 204 bits (520), Expect = 3e-50
Identities = 106/279 (37%), Positives = 171/279 (61%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG + + DD + E ++ AI E I++ L + F + +Y+P K + E KQ
Sbjct: 195 RLVFGQDFN-DDKYVEDLHQAIEELIRMSGYAQLAEAFYYAKYLPSHKKAIRHAELTKQI 253
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
V L+ ++S + P++S YL FL SQ + E++VI +FE ++ VDSTS+T AWALA++
Sbjct: 254 VTNLVRPFLSLNPPTNS--YLHFLQSQKYDEEMVIFAIFETYLLGVDSTSSTTAWALAYL 311
Query: 362 IHHEDIQQRVLTQVMNKLGGTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRRAT 541
+ ++Q R+ ++ N R + ++ +++Y+ AV KET+R +PI P +P +A
Sbjct: 312 VREPNVQDRLYNELNNYANYNDRKILKVEVINKLQYLQAVTKETMRMKPIAPLAIPHKAC 371
Query: 542 RDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDVTRMERSF 721
RD+ L G + +GT+V+VNL+A+ H+E V+ +P KFMPERFL + + ++ ME+S
Sbjct: 372 RDTSLMGTKIDKGTKVMVNLHALHHNENVFTDPFKFMPERFLK--VNNEDVNMKAMEQSL 429
Query: 722 ISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEG 838
+ F AG R C GMEL K + + LANLV +F+W +G
Sbjct: 430 LPFSAGMRICAGMELGKLQFSFALANLVYAFKWSCVDDG 468
>sp|Q50LH4.1|C7193_ESCCA RecName: Full=(S)-stylopine synthase 2; Short=STS; AltName:
Full=Cytochrome P450 719A3; Short=EcCYP719A3;
Short=EcCYPA gi|63108254|dbj|BAD98249.1| stylopine
synthase [Eschscholzia californica]
Length = 495
Score = 204 bits (520), Expect = 3e-50
Identities = 111/283 (39%), Positives = 175/283 (61%), Gaps = 4/283 (1%)
Frame = +2
Query: 2 RLCFGPESSMDDLFAEGIYCAIVEAIKLEEKGHLVDVFEFTRYIPGLKPPFKELESLKQR 181
RL FG + +D + E ++ AI E I++ L + F + +Y+P + + +E LKQ
Sbjct: 195 RLVFGRDFE-EDKYVEDMHHAIEELIRISGYARLAEAFYYAKYLPSHRKAVRYVEELKQI 253
Query: 182 VKELISSYISTHKPSSSNCYLSFLLSQGFTEDIVIANLFELFMFSVDSTSNTIAWALAFV 361
VK LI ++S + P+ N YL FL SQ + E++VI +FE ++ VDSTS+T AWALA++
Sbjct: 254 VKNLIRPFLSVNPPT--NTYLHFLRSQNYDEEVVIFAIFETYLLGVDSTSSTTAWALAYL 311
Query: 362 IHHEDIQQRVLTQVMNKLG--GTRRMVSVEDLKTEMEYVHAVVKETVRSRPIVPWGLPRR 535
+ +Q R L Q ++ R+++ VED+ +++Y+ AV+KET+R +PI P +P +
Sbjct: 312 VREPSVQDR-LHQELDHFAKQNDRKILKVEDMN-KLQYLQAVIKETMRMKPIAPLAIPHK 369
Query: 536 ATRDSELKGMTVREGTQVLVNLYAVLHDERVWKEPNKFMPERFLMSGSSGDGEDV--TRM 709
A +D+ L G + +GT+V+VNLYA+ H++ V+ +P KFMPERFL D +D M
Sbjct: 370 ACKDTSLMGNKINKGTRVMVNLYALHHNKNVFNDPFKFMPERFL----KVDNQDAKGKAM 425
Query: 710 ERSFISFGAGRRSCPGMELAKAEIALTLANLVNSFQWCSAVEG 838
E+S + F AG R C GMEL K + + LANL+ +F+W +G
Sbjct: 426 EQSLLPFSAGMRICAGMELGKLQFSFALANLIFAFKWSCVDDG 468