BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cocculus22_contig00007069
         (357 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransf...    75   1e-11
ref|XP_006346269.1| PREDICTED: trans-resveratrol di-O-methyltran...    56   6e-06
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra...    55   8e-06

>sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransferase; Short=CoOMT;
           AltName: Full=Tetrahydrocolumbamine
           2-O-methyltransferase gi|24210466|dbj|BAC22084.1|
           columbamine O-methyltransferase [Coptis japonica]
          Length = 351

 Score = 75.1 bits (183), Expect = 1e-11
 Identities = 51/130 (39%), Positives = 70/130 (53%), Gaps = 13/130 (10%)
 Frame = -2

Query: 356 KDYYGLTPVSKILLKDHKRSLAPLLML-------------IEGLDGSSSTTFWESHYGLP 216
           +D Y LTP SK+L+  H++SLAP +ML             I  LDG     +WES+    
Sbjct: 92  EDKYELTPASKLLVHGHQKSLAPYVMLQTHPEEFSVWSHVINVLDGKK--PYWESN-DTS 148

Query: 215 FYEMVEKDPKWNQKMINGMTTITMCMVDALVDGLKRGKVIDDDEHEGTARTVTSLVDVGG 36
            YE  E DP+ N+ + + MT+ +  M+ ALV GL +  V+D          V S+VDVGG
Sbjct: 149 MYEKTEGDPEINEILNDAMTSHSTFMLPALVSGLMKENVLDG---------VASIVDVGG 199

Query: 35  NKGNVAKAIL 6
           N G VAK I+
Sbjct: 200 NSGVVAKGIV 209


>ref|XP_006346269.1| PREDICTED: trans-resveratrol di-O-methyltransferase-like [Solanum
           tuberosum]
          Length = 364

 Score = 55.8 bits (133), Expect = 6e-06
 Identities = 38/129 (29%), Positives = 59/129 (45%), Gaps = 16/129 (12%)
 Frame = -2

Query: 347 YGLTPVSKILLKDHKRSLAPLLML----------------IEGLDGSSSTTFWESHYGLP 216
           Y LTP S++LLKD   S+ P L                     +   S+TT + + +G+P
Sbjct: 99  YLLTPTSRLLLKDEPMSMIPFLNFQLDPNLMDPWHSLSKWFNNVSDDSNTTPYATAHGMP 158

Query: 215 FYEMVEKDPKWNQKMINGMTTITMCMVDALVDGLKRGKVIDDDEHEGTARTVTSLVDVGG 36
           F++  E +P+ N      M + T  ++  L+   K G + +          + SLVDVGG
Sbjct: 159 FFKYAENEPRLNHLFNEAMASDTRLVMTVLIQNGK-GLIFEG---------LKSLVDVGG 208

Query: 35  NKGNVAKAI 9
             G +AKAI
Sbjct: 209 GTGTIAKAI 217


>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase
           [Thalictrum flavum subsp. glaucum]
          Length = 348

 Score = 55.5 bits (132), Expect = 8e-06
 Identities = 37/128 (28%), Positives = 60/128 (46%), Gaps = 13/128 (10%)
 Frame = -2

Query: 353 DYYGLTPVSKILLKDHKRSLAPLLM-------------LIEGLDGSSSTTFWESHYGLPF 213
           D Y L P++K++L++ KRS+ P+++             + +GL  +  T F E   G+  
Sbjct: 89  DKYALEPIAKLVLRNEKRSMVPMVLGMTQKDFMTPWHSMKDGLTDNGVTAF-EKAMGMTI 147

Query: 212 YEMVEKDPKWNQKMINGMTTITMCMVDALVDGLKRGKVIDDDEHEGTARTVTSLVDVGGN 33
           +E +E  P  +Q    GM   T  +  +L+ G K              +++ SLVDVGG 
Sbjct: 148 WEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSK-----------DMFQSIDSLVDVGGG 196

Query: 32  KGNVAKAI 9
            G   KAI
Sbjct: 197 NGTTIKAI 204