BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cocculus22_contig00006701
         (999 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]                    351   2e-94
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   347   3e-93
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN...   298   2e-78
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              267   6e-69
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   266   1e-68
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   264   5e-68
emb|CAN62646.1| hypothetical protein VITISV_013218 [Vitis vinifera]   263   6e-68
ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   263   8e-68
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   262   2e-67
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]   262   2e-67
emb|CBI34714.3| unnamed protein product [Vitis vinifera]              261   2e-67
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   261   2e-67
ref|XP_002274586.2| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   261   3e-67
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   261   3e-67
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum...   261   4e-67
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   261   4e-67
ref|XP_002509713.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   259   1e-66
emb|CBI34715.3| unnamed protein product [Vitis vinifera]              259   2e-66
gb|EXC34114.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [...   258   2e-66
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   258   2e-66

>gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]
          Length = 346

 Score =  351 bits (901), Expect = 2e-94
 Identities = 171/225 (76%), Positives = 194/225 (86%)
 Frame = +3

Query: 54  ESKDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELD 233
           E KDMLDVLL N+F D QIN +FLETFGPGSETSSAT EWA+AELIKNP  L+KVRQELD
Sbjct: 122 EPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIKNPHILAKVRQELD 181

Query: 234 QVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKII 413
           QVVG KS VKE+ +PHLPYLQACIKETMRLHPAAPFLLPHRA ETC VMGYT+PKD +++
Sbjct: 182 QVVG-KSTVKESDLPHLPYLQACIKETMRLHPAAPFLLPHRAVETCPVMGYTIPKDTQVL 240

Query: 414 VNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPL 593
           VN YAIGRDP TW +P+TF PERFLES+VDY G HFEFIPFG+GRR CVG+PLA RTIPL
Sbjct: 241 VNIYAIGRDPKTWKEPTTFKPERFLESDVDYYGKHFEFIPFGAGRRQCVGMPLATRTIPL 300

Query: 594 IVASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVR 728
           IV++LV    WSLPD K P++L MNE LSLSLA+DP+L++IPK R
Sbjct: 301 IVSNLVQTLDWSLPDGKLPQDLVMNESLSLSLALDPTLTVIPKSR 345


>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  347 bits (891), Expect = 3e-93
 Identities = 164/225 (72%), Positives = 196/225 (87%)
 Frame = +3

Query: 54  ESKDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELD 233
           ESKDMLDVLL N+F D QIN LFLETFGPGSETSSATIEW +AELIK+P +++KVR+EL+
Sbjct: 262 ESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELN 321

Query: 234 QVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKII 413
           +VVG  S +KE+ +P LPYLQACIKE MRLHPAAPFLLP RA ETC+VMGYT+PK+ +++
Sbjct: 322 EVVGT-STIKESDLPQLPYLQACIKEAMRLHPAAPFLLPRRAAETCEVMGYTIPKNSQVL 380

Query: 414 VNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPL 593
           VNAYAIGRDP +W DPSTFWPERFLES+VD++G H++FIPFGSGRR CVG+PLA RTIPL
Sbjct: 381 VNAYAIGRDPKSWKDPSTFWPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPL 440

Query: 594 IVASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVR 728
           IV SLVHN+ + LP   RPE+L MNE+LSL+LAIDPSL ++PK R
Sbjct: 441 IVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLAIDPSLCVVPKAR 485


>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
           Full=(S)-N-methylcoclaurine oxidase [C-O
           phenol-coupling]; AltName: Full=CYPLXXX; AltName:
           Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
           cytochrome P-450 CYP80 [Berberis stolonifera]
          Length = 487

 Score =  298 bits (763), Expect = 2e-78
 Identities = 141/225 (62%), Positives = 174/225 (77%)
 Frame = +3

Query: 54  ESKDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELD 233
           E KDMLDVL+ N+F + QIN +F+ETFGPGS+T+S  IEWA+A+LIKNP KL+K+R+ELD
Sbjct: 262 EPKDMLDVLIANDFNEHQINAMFMETFGPGSDTNSNIIEWALAQLIKNPDKLAKLREELD 321

Query: 234 QVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKII 413
           +VVG  S VKE+H   LPYLQAC+KETMRL+P    ++PHR  ETCQVMGYT+PK   + 
Sbjct: 322 RVVGRSSTVKESHFSELPYLQACVKETMRLYPPISIMIPHRCMETCQVMGYTIPKGMDVH 381

Query: 414 VNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPL 593
           VNA+AIGRDP  W DP  F PERFL+S+++YNG  F+FIPFGSGRRIC G PLA R IPL
Sbjct: 382 VNAHAIGRDPKDWKDPLKFQPERFLDSDIEYNGKQFQFIPFGSGRRICPGRPLAVRIIPL 441

Query: 594 IVASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVR 728
           ++ASLVH F W LPD    E+L M E+ +LSL +   L +IPKVR
Sbjct: 442 VLASLVHAFGWELPDGVPNEKLDMEELFTLSLCMAKPLRVIPKVR 486


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  267 bits (682), Expect = 6e-69
 Identities = 122/224 (54%), Positives = 168/224 (75%)
 Frame = +3

Query: 60   KDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQV 239
            +D+L+  L   F D QIN + LE F  G++TS+ TIEWA+ +LI+NP  + K+R EL ++
Sbjct: 1316 RDLLEAFLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKI 1375

Query: 240  VGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVN 419
            +G +S V+E+H+PHLPYLQAC+KET+RLHP AP LLPHRA ETCQVMGYT+PKD ++ VN
Sbjct: 1376 IG-ESPVRESHLPHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVN 1434

Query: 420  AYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIV 599
             +A+GRDP  W DP +F PERFL+S +++ GN FE+IPFG+GRRIC G+ L  R +PL++
Sbjct: 1435 IWAMGRDPKVWDDPLSFTPERFLDSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVL 1494

Query: 600  ASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVRE 731
            A+LVH F WSLPD     ++ M E L ++L  +  L ++PKVR+
Sbjct: 1495 ATLVHLFDWSLPDNMDSAQIDMEEWLVITLRKENPLRLVPKVRK 1538



 Score =  263 bits (671), Expect = 1e-67
 Identities = 126/225 (56%), Positives = 163/225 (72%)
 Frame = +3

Query: 60   KDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQV 239
            +D L+ LL   F D QIN + LE F  G+ETSS T+EWAMAELI+N   + K+R EL Q+
Sbjct: 816  RDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQI 875

Query: 240  VGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVN 419
            VG +S V+E+H+P LPYLQAC+KE +RLHP AP LLPH A ETCQVMGYT+PKD +I VN
Sbjct: 876  VG-ESPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVN 934

Query: 420  AYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIV 599
             +A+ RDP  W DP +F PERFL+S +D+ GN FE+IPFG+GRRIC GL L  R +PLI+
Sbjct: 935  IWAMARDPKIWDDPLSFKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLIL 994

Query: 600  ASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVREH 734
            A+ VH F WSLP      +L M E L ++L  +  L ++P+VR++
Sbjct: 995  ATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKEQPLRLVPRVRKY 1039



 Score =  254 bits (650), Expect = 3e-65
 Identities = 121/220 (55%), Positives = 158/220 (71%)
 Frame = +3

Query: 63  DMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQVV 242
           D LD LL   F D QIN + +E F  G ETSS T+EWAMAELIKN   + K+  EL Q++
Sbjct: 276 DFLDALLETGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQII 335

Query: 243 GPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVNA 422
           G +S V+E+H+PHLPYLQAC+KET+RLHP  P LLPHRA ETCQ+MGYT+PKD  I VN 
Sbjct: 336 G-ESPVRESHLPHLPYLQACVKETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNM 394

Query: 423 YAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIVA 602
           +A+GRDP TW DP +F PERFL+S +++ GN FE+IPFG+GRR+C G+PLA R +P+I+A
Sbjct: 395 WAMGRDPGTWEDPLSFKPERFLDSKLEFKGNDFEYIPFGAGRRMCPGMPLAARLVPMILA 454

Query: 603 SLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPK 722
           + V  F WS P +    E+ M E   ++L  +  L ++ K
Sbjct: 455 TFVRLFDWSTPGDMDFAEIDMEERFVITLRKEQPLRLLKK 494


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  266 bits (679), Expect = 1e-68
 Identities = 133/224 (59%), Positives = 163/224 (72%)
 Frame = +3

Query: 57  SKDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQ 236
           + D +DVLL+    D +IN L +E FG G+ETS++TIEWA+ EL K P+ +SK+R EL  
Sbjct: 262 NNDFVDVLLNAGLEDQKINALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVN 321

Query: 237 VVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIV 416
           VVG  + VKE+ +PHLPYLQA +KET+RLHP  P LLP RA ETC VM YT+PK+ +I+V
Sbjct: 322 VVGDNT-VKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMV 380

Query: 417 NAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLI 596
           NA+AIGRDP TW DP  F PERFL S+VDY GN FE IPFG GRRIC GLPLA++ I LI
Sbjct: 381 NAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFISLI 440

Query: 597 VASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVR 728
           VA+LV NF WSLP      EL+M+E   L+L  DP L I+ K R
Sbjct: 441 VATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 484


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
           isozyme 2; AltName: Full=Cytochrome P450 80B2
           gi|9971208|dbj|BAB12433.1|
           (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  264 bits (674), Expect = 5e-68
 Identities = 135/226 (59%), Positives = 164/226 (72%), Gaps = 1/226 (0%)
 Frame = +3

Query: 54  ESK-DMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQEL 230
           ESK D +DVLL+    D +IN L +E FG G+ETS++TIEWA+ EL K P+ +SK+R EL
Sbjct: 260 ESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLEL 319

Query: 231 DQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKI 410
             VVG  + VKE+ +PHLPYLQA +KET+RLHP  P LLP RA ETC VM YT+PK+ +I
Sbjct: 320 VNVVGDNT-VKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQI 378

Query: 411 IVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIP 590
           +VNA+AIGRDP TW DP  F PERFL S+VDY GN FE IPFG GRRIC GLPLA++   
Sbjct: 379 MVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFSN 438

Query: 591 LIVASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVR 728
           LIVA+LV NF WSLP      EL+M+E   L+L  DP L I+ K R
Sbjct: 439 LIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 484


>emb|CAN62646.1| hypothetical protein VITISV_013218 [Vitis vinifera]
          Length = 475

 Score =  263 bits (673), Expect = 6e-68
 Identities = 121/224 (54%), Positives = 166/224 (74%)
 Frame = +3

Query: 60  KDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQV 239
           +D+L+  L   F D QIN + LE F  G++TS+ TIEWA+ +LI+NP  + K+R EL ++
Sbjct: 253 RDLLEAFLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKI 312

Query: 240 VGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVN 419
           +G +S V+E+H+PHLPYLQAC+KET+RLHP AP LLPHRA ETCQVMGYT+PKD ++ VN
Sbjct: 313 IG-ESPVRESHLPHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVN 371

Query: 420 AYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIV 599
            +A+GRDP  W  P +F PERFL+S +++ GN FE+IPFG GRRIC G+ L  R +PL++
Sbjct: 372 IWAMGRDPKVWDXPLSFTPERFLDSKLEFKGNDFEYIPFGXGRRICPGMALGARQVPLVL 431

Query: 600 ASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVRE 731
           A+LVH F WSLPD     ++ M E L ++L  +  L ++PKVR+
Sbjct: 432 ATLVHLFDWSLPDNMDSAQIDMEEWLVITLRKENPLRLVPKVRK 475


>ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
           [Vitis vinifera]
          Length = 515

 Score =  263 bits (672), Expect = 8e-68
 Identities = 120/221 (54%), Positives = 165/221 (74%)
 Frame = +3

Query: 60  KDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQV 239
           +D+L+  L   F D QIN + LE F  G++TS+ TIEWA+ +LI+NP  + K+R EL ++
Sbjct: 271 RDLLEAFLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKI 330

Query: 240 VGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVN 419
           +G +S V+E+H+PHLPYLQAC+KET+RLHP AP LLPHRA ETCQVMGYT+PKD ++ VN
Sbjct: 331 IG-ESPVRESHLPHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVN 389

Query: 420 AYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIV 599
            +A+GRDP  W DP +F PERFL+S +++ GN FE+IPFG+GRRIC G+ L  R +PL++
Sbjct: 390 IWAMGRDPKVWDDPLSFTPERFLDSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVL 449

Query: 600 ASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPK 722
           A+LVH F WSLPD     ++ M E L ++L  +  L ++PK
Sbjct: 450 ATLVHLFDWSLPDNMDSAQIDMEEWLVITLRKENPLRLVPK 490


>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 498

 Score =  262 bits (669), Expect = 2e-67
 Identities = 126/224 (56%), Positives = 162/224 (72%)
 Frame = +3

Query: 60  KDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQV 239
           +D L+ LL   F D QIN + LE F  G+ETSS T+EWAMAELI+N   + K+R EL Q+
Sbjct: 276 RDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQI 335

Query: 240 VGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVN 419
           VG +S V+E+H+P LPYLQAC+KE +RLHP AP LLPH A ETCQVMGYT+PKD +I VN
Sbjct: 336 VG-ESPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVN 394

Query: 420 AYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIV 599
            +A+ RDP  W DP +F PERFL+S +D+ GN FE+IPFG+GRRIC GL L  R +PLI+
Sbjct: 395 IWAMARDPKIWDDPLSFKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLIL 454

Query: 600 ASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVRE 731
           A+ VH F WSLP      +L M E L ++L  +  L ++P+VR+
Sbjct: 455 ATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKEQPLRLVPRVRK 498


>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
          Length = 498

 Score =  262 bits (669), Expect = 2e-67
 Identities = 126/224 (56%), Positives = 162/224 (72%)
 Frame = +3

Query: 60  KDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQV 239
           +D L+ LL   F D QIN + LE F  G+ETSS T+EWAMAELI+N   + K+R EL Q+
Sbjct: 276 RDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQI 335

Query: 240 VGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVN 419
           VG +S V+E+H+P LPYLQAC+KE +RLHP AP LLPH A ETCQVMGYT+PKD +I VN
Sbjct: 336 VG-ESPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVN 394

Query: 420 AYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIV 599
            +A+ RDP  W DP +F PERFL+S +D+ GN FE+IPFG+GRRIC GL L  R +PLI+
Sbjct: 395 IWAMARDPKIWDDPLSFKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLIL 454

Query: 600 ASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVRE 731
           A+ VH F WSLP      +L M E L ++L  +  L ++P+VR+
Sbjct: 455 ATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKEQPLRLVPRVRK 498


>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
          Length = 936

 Score =  261 bits (668), Expect = 2e-67
 Identities = 126/224 (56%), Positives = 165/224 (73%)
 Frame = +3

Query: 60   KDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQV 239
            +D L++L+ + + D QIN LF+E    G++TSS+T+EWAMAELIK+P  + KV +EL + 
Sbjct: 714  QDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELARE 773

Query: 240  VGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVN 419
            +      K + +PHLPYLQAC+KET+RLHP+AP LLP RA  +C+VM YT+PKD +I VN
Sbjct: 774  ISDNLP-KASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVN 832

Query: 420  AYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIV 599
            A+AIGRDP  W DP  F PERFL S VD+ GN+ EFIPFG+GRRIC GLP+A R +PLI+
Sbjct: 833  AWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLIL 892

Query: 600  ASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVRE 731
            ASL H F WSLP+   P+EL MN+   ++L  +  L IIPKVR+
Sbjct: 893  ASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 936


>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
           2-like [Vitis vinifera]
          Length = 503

 Score =  261 bits (668), Expect = 2e-67
 Identities = 126/224 (56%), Positives = 165/224 (73%)
 Frame = +3

Query: 60  KDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQV 239
           +D L++L+ + + D QIN LF+E    G++TSS+T+EWAMAELIK+P  + KV +EL + 
Sbjct: 281 QDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELARE 340

Query: 240 VGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVN 419
           +      K + +PHLPYLQAC+KET+RLHP+AP LLP RA  +C+VM YT+PKD +I VN
Sbjct: 341 ISDNLP-KASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVN 399

Query: 420 AYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIV 599
           A+AIGRDP  W DP  F PERFL S VD+ GN+ EFIPFG+GRRIC GLP+A R +PLI+
Sbjct: 400 AWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLIL 459

Query: 600 ASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVRE 731
           ASL H F WSLP+   P+EL MN+   ++L  +  L IIPKVR+
Sbjct: 460 ASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 503


>ref|XP_002274586.2| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
           [Vitis vinifera]
          Length = 498

 Score =  261 bits (667), Expect = 3e-67
 Identities = 122/224 (54%), Positives = 163/224 (72%)
 Frame = +3

Query: 63  DMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQVV 242
           D LD LL   F D QIN + +E F  G ETSS T+EWAMAELIKN   + K+  EL Q++
Sbjct: 276 DFLDALLETGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQII 335

Query: 243 GPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVNA 422
           G +S V+E+H+PHLPYLQAC+KET+RLHP  P LLPHRA ETCQ+MGYT+PKD  I VN 
Sbjct: 336 G-ESPVRESHLPHLPYLQACVKETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNM 394

Query: 423 YAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIVA 602
           +A+GRDP TW DP +F PERFL+S +++ GN FE+IPFG+GRR+C G+PLA R +P+I+A
Sbjct: 395 WAMGRDPGTWEDPLSFKPERFLDSKLEFKGNDFEYIPFGAGRRMCPGMPLAARLVPMILA 454

Query: 603 SLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVREH 734
           + V  F WS P +    E+ M E   ++L  +  L ++P++R++
Sbjct: 455 TFVRLFDWSTPGDMDFAEIDMEERFVITLRKEQPLRLVPRIRKY 498


>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
           [Vitis vinifera]
          Length = 503

 Score =  261 bits (667), Expect = 3e-67
 Identities = 126/224 (56%), Positives = 165/224 (73%)
 Frame = +3

Query: 60  KDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQV 239
           +D L++L+ + + D QIN LF+E    G++TSS+T+EWAMAELIK+P  + KV +EL + 
Sbjct: 281 QDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELARE 340

Query: 240 VGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVN 419
           +      K + +PHLPYLQAC+KET+RLHP+AP LLP RA  +C+VM YT+PKD +I VN
Sbjct: 341 ISDNLP-KASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVN 399

Query: 420 AYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIV 599
           A+AIGRDP  W DP  F PERFL S VD+ GN+ EFIPFG+GRRIC GLP+A R +PLI+
Sbjct: 400 AWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLIL 459

Query: 600 ASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVRE 731
           ASL H F WSLP+   P+EL MN+   ++L  +  L IIPKVR+
Sbjct: 460 ASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLIIPKVRK 503


>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
           glaucum]
          Length = 491

 Score =  261 bits (666), Expect = 4e-67
 Identities = 131/226 (57%), Positives = 162/226 (71%)
 Frame = +3

Query: 63  DMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQVV 242
           D  +VLL+    D +IN L +E FG G+ET+++TIEWA+ EL  N   +SK+R EL  VV
Sbjct: 264 DFANVLLNAGLDDQKINALLMELFGAGTETTASTIEWAITELTNNLRVISKLRAELINVV 323

Query: 243 GPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVNA 422
           G K+ VKE++IPHLPYLQA +KET+RLHP  P LLP RA ETC+VM YT+PK+ +I+VNA
Sbjct: 324 GHKT-VKESNIPHLPYLQAIVKETLRLHPPTPLLLPRRALETCKVMNYTIPKECQIMVNA 382

Query: 423 YAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIVA 602
           +AIGRDP TW DP TF PERF+ S VDY GN FE IPFG GRRIC GLPLA++ + LIVA
Sbjct: 383 WAIGRDPKTWDDPLTFKPERFMNSTVDYKGNDFELIPFGGGRRICPGLPLASQFLSLIVA 442

Query: 603 SLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVREHIK 740
           +LV NF WS P    P E+ M+E   L L  DP L I+ K R  +K
Sbjct: 443 TLVQNFDWSFPQGMTPNEVPMDEKFGLPLQKDPPLLIVLKARTSMK 488


>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
           communis] gi|223547255|gb|EEF48750.1|
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
           [Ricinus communis]
          Length = 496

 Score =  261 bits (666), Expect = 4e-67
 Identities = 123/225 (54%), Positives = 162/225 (72%)
 Frame = +3

Query: 48  EGESKDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQE 227
           +    D LDV L N F D QIN L LE F  G++T++ T+EWAMAEL+K    L KVR+E
Sbjct: 270 DARKTDFLDVFLSNGFDDHQINWLLLELFSAGADTTTTTVEWAMAELLKEITVLEKVREE 329

Query: 228 LDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYK 407
           L+  +G K  ++E+HIP L YL AC+KET+RLHP  PFL+P RA E C+VM YT+PK  +
Sbjct: 330 LETEIG-KDMIRESHIPQLKYLNACVKETLRLHPPVPFLIPRRAPEACEVMNYTIPKHSQ 388

Query: 408 IIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTI 587
           IIVN +AIGRDP+ W DP ++ PERFL+SN+D+ G++FEF+PFGSGRRIC GLP+  R +
Sbjct: 389 IIVNVWAIGRDPSAWEDPLSYRPERFLDSNLDFKGHNFEFLPFGSGRRICPGLPMGTRQL 448

Query: 588 PLIVASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPK 722
           PLI+ASLVH F WSL +   P  L MN+  S++L  +  L ++PK
Sbjct: 449 PLILASLVHCFDWSLQNGDDPAMLDMNDKFSITLEKEQHLLVVPK 493


>ref|XP_002509713.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
           communis] gi|223549612|gb|EEF51100.1|
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
           [Ricinus communis]
          Length = 501

 Score =  259 bits (662), Expect = 1e-66
 Identities = 127/222 (57%), Positives = 163/222 (73%)
 Frame = +3

Query: 63  DMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQVV 242
           D LD L++N   + QINVL LE    G++TSS TIEW MAEL+KNP  + KV++E+ + +
Sbjct: 273 DFLDTLINNGSSNQQINVLLLELLSAGTDTSSNTIEWTMAELMKNPKCMKKVQEEITRNL 332

Query: 243 GPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVNA 422
            P   +KE+ I +L YLQAC+KET+RLHP  PFLLPHRA +TCQVM YT+PK+ +++VN 
Sbjct: 333 IPDI-LKESPISNLTYLQACVKETLRLHPPGPFLLPHRATDTCQVMNYTIPKNSQVLVNF 391

Query: 423 YAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIVA 602
           +AIGRDP  W DP  F PERFL SN+DY GN FEFIPFGSGRRIC GLP+A + +PLIVA
Sbjct: 392 WAIGRDPKYWKDPLIFKPERFLNSNLDYKGNDFEFIPFGSGRRICPGLPMAAKQVPLIVA 451

Query: 603 SLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVR 728
           SL+H F WSLP  K   +L M E   L+L ++  L +IPK++
Sbjct: 452 SLIHFFDWSLPGGKDSIDLDMTEKYGLTLRMEKPLLLIPKIK 493


>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
          Length = 1076

 Score =  259 bits (661), Expect = 2e-66
 Identities = 125/224 (55%), Positives = 164/224 (73%)
 Frame = +3

Query: 60   KDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQV 239
            +D L++L+ + + D QIN LF+E    G++TSS+T+EWAMAELIK+P  + KV +EL + 
Sbjct: 845  QDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELARE 904

Query: 240  VGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVN 419
            +      K + +PHLPYLQAC+KET+RLHP+AP LLP RA  +C+VM YT+PKD +I VN
Sbjct: 905  ISDNLP-KASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVN 963

Query: 420  AYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIV 599
            A+AIGRDP  W DP  F PERFL S VD+ GN+ EFIPFG+GRRIC GLP+A R +PLI+
Sbjct: 964  AWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLIL 1023

Query: 600  ASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVRE 731
            ASL H F WSLP+   P+EL MN+   ++L  +  L IIPK R+
Sbjct: 1024 ASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLIIPKKRK 1067


>gb|EXC34114.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Morus notabilis]
          Length = 515

 Score =  258 bits (660), Expect = 2e-66
 Identities = 119/223 (53%), Positives = 163/223 (73%)
 Frame = +3

Query: 63  DMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELDQVV 242
           D LD +L N + D QIN+L LE F  G+++SS T+EW MAEL++NP  +  +R+EL++ +
Sbjct: 293 DFLDAMLENGYPDFQINILLLELFSAGTDSSSTTVEWLMAELVRNPKCMKILREELEREI 352

Query: 243 GPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKIIVNA 422
           G +  V E  +P L YL+ C+KE +RL P AP  +PHRA ETCQVM YTVPK+ +I+VN 
Sbjct: 353 GSQEMVMECDLPKLKYLECCLKEPLRLPPPAPLPIPHRAAETCQVMNYTVPKNSEILVNV 412

Query: 423 YAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPLIVA 602
           +AIGRDPN W DP  F PERFL S++D+ G +FE++PFG+GRRIC G PLA++ +PLIVA
Sbjct: 413 WAIGRDPNHWEDPLEFKPERFLNSSLDFKGTNFEYLPFGAGRRICPGQPLASKQVPLIVA 472

Query: 603 SLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVRE 731
           SLVH+F WSLP+ K P+EL M E   +++     L ++PKVR+
Sbjct: 473 SLVHSFDWSLPNGKDPKELDMAEKYDINMLKKEPLLVVPKVRK 515


>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  258 bits (660), Expect = 2e-66
 Identities = 126/226 (55%), Positives = 163/226 (72%)
 Frame = +3

Query: 54  ESKDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPIKLSKVRQELD 233
           +  D +++LL +   D QIN L +E FG G+ETS++TIEWA++EL KNP   + +R EL 
Sbjct: 257 KKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELL 316

Query: 234 QVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGYTVPKDYKII 413
            VVG K  VKE+ IP++PYLQA +KET+RLHPA P LLP RA ETC+V+ YT+PK+ +I+
Sbjct: 317 SVVG-KRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIM 375

Query: 414 VNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGLPLANRTIPL 593
           VNA+ IGRDP  WTDP  F PERFL S++D+ GN FE IPFG+GRRIC G+PLA + I L
Sbjct: 376 VNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISL 435

Query: 594 IVASLVHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVRE 731
           IV+SLV NF W LP    P +L M E   L+L  +P L I+PK R+
Sbjct: 436 IVSSLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKTRD 481