BLASTX nr result
ID: Cocculus22_contig00006647
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cocculus22_contig00006647
(877 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss... 260 4e-67
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 160 8e-37
ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 160 8e-37
ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 157 7e-36
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 156 1e-35
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera] 155 1e-35
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 153 7e-35
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 151 3e-34
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN... 150 6e-34
ref|XP_006358870.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 150 6e-34
ref|XP_004234364.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 149 2e-33
ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 147 4e-33
ref|XP_002318833.1| hypothetical protein POPTR_0012s13580g [Popu... 146 9e-33
ref|XP_006358915.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 146 1e-32
ref|XP_006353349.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 145 2e-32
ref|XP_004234365.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 145 3e-32
ref|XP_006353351.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 144 6e-32
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 142 2e-31
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 140 8e-31
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 139 2e-30
>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 260 bits (665), Expect = 4e-67
Identities = 150/274 (54%), Positives = 181/274 (66%), Gaps = 15/274 (5%)
Frame = +1
Query: 100 IVVLYLLFKPKH-NLPPGPRPSWPLIGNLPTILGSNT--PLHVTLMNLARAHGPLMLVTL 270
I+V LL KPK+ NLPPGP P WPLIGNLP IL +NT PLH+TL N+AR HGP+M++ L
Sbjct: 14 ILVFILLLKPKYKNLPPGPHP-WPLIGNLP-ILFTNTEVPLHITLANMARTHGPIMILWL 71
Query: 271 GTQPXXXXXXXXXX-----------SGRHIAKSFRIKEMNKHSLVWANCTDNWKLLRKLA 417
GTQP S RHI SFR+K K+SLVW++CTD WKLLRK+
Sbjct: 72 GTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIV 131
Query: 418 RTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGN-XXXXXXX 594
RTEIFS +MLQ Q VRE KV EL++FLR +EG+VVKIS+FVFGTLLNI+GN
Sbjct: 132 RTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVF 191
Query: 595 XXXXXXXRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLWEPT 774
+ G+Q+LI+E+LMIGA PN+AEFYP LE LDLQGLK++C +R +V K+WE T
Sbjct: 192 VYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFIRVMKMWEGT 251
Query: 775 VKEXXXXXXXXXXXXXXXXXEGESKDMLDVLLDN 876
VKE ESKDMLDVLL N
Sbjct: 252 VKE------------RKANRNEESKDMLDVLLAN 273
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 160 bits (404), Expect = 8e-37
Identities = 98/244 (40%), Positives = 141/244 (57%), Gaps = 14/244 (5%)
Frame = +1
Query: 94 FAIVVLYLLFKPKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLG 273
F +++ ++ KP LPPGP P WP+IGNL LG N HV L +LA+ HGPLM + LG
Sbjct: 1069 FLLILKHIFLKPPP-LPPGPYP-WPIIGNLLQ-LGKNP--HVKLASLAKLHGPLMSLRLG 1123
Query: 274 TQPXXXXXXXXXX-----------SGRHIAKSFRIKE--MNKHSLVWAN-CTDNWKLLRK 411
TQ SGR+++ S +K+ +N SL +A CT+NWK LR
Sbjct: 1124 TQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRT 1183
Query: 412 LARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXXXXXX 591
+ RTE+FS + ++ +RE KV ELVEFL +EG+VVK+ VF T+ NI+ N
Sbjct: 1184 ICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSN---TFF 1240
Query: 592 XXXXXXXXRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLWEP 771
R G++ I +GA+PNL++FYPIL+GL+L G K++ + +G++ WE
Sbjct: 1241 SMDLCDFEREGLKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALGRILATWEG 1300
Query: 772 TVKE 783
T+KE
Sbjct: 1301 TLKE 1304
Score = 156 bits (394), Expect = 1e-35
Identities = 95/246 (38%), Positives = 139/246 (56%), Gaps = 16/246 (6%)
Frame = +1
Query: 94 FAIVVLYLLFKPKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLG 273
F +++ ++ KP LPPGP P WP+IGNL +G N H L NLA+ HGPLM + LG
Sbjct: 564 FLVILKHIFLKPPP-LPPGPYP-WPIIGNLLQ-MGKNP--HAKLANLAKLHGPLMSLRLG 618
Query: 274 TQ-----------PXXXXXXXXXXSGRHIAKSFRIK--EMNKHSLVWA-NCTDNWKLLRK 411
TQ SGR+++ S +K ++N S+V+A +C NWK LR
Sbjct: 619 TQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRA 678
Query: 412 LARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXXXXXX 591
+ R E+FS + ++ Q +RE KV ELVEFL +EG+VVK+ VF T+ NI+ N
Sbjct: 679 ICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMD 738
Query: 592 XXXXXXXXRIG--MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLW 765
R+G ++ LI + GA+PNL+++YPIL GLD+QG+ R+ + ++ W
Sbjct: 739 LCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTW 798
Query: 766 EPTVKE 783
E +KE
Sbjct: 799 EDILKE 804
Score = 132 bits (332), Expect = 2e-28
Identities = 82/244 (33%), Positives = 134/244 (54%), Gaps = 16/244 (6%)
Frame = +1
Query: 100 IVVLYLLFKPKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLGTQ 279
+++ +F LPPGP P WP+IGNL + G+ LHV L NLA+ HGPLM + LGTQ
Sbjct: 25 LLIFKHIFLKSPPLPPGPYP-WPIIGNLLQMGGN---LHVKLANLAKRHGPLMSLRLGTQ 80
Query: 280 -----------PXXXXXXXXXXSGRHIAKSFRIK--EMNKHSLVWAN-CTDNWKLLRKLA 417
SGR+++ + + ++N ++ +A C +W+ L+ +
Sbjct: 81 IMVVASSSAAAMEVLKTHDRTLSGRYVSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAIC 140
Query: 418 RTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXXXXXXXX 597
R E+FS + ++ + +RE KV ELVEFL ++EG+VVK+ V+ T+ NI+ N
Sbjct: 141 RMELFSGKAMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDFS 200
Query: 598 XXXXXXRIG--MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLWEP 771
G ++ L+ E +GA+ N+ +FYPIL GLD+QG++++ + ++ WE
Sbjct: 201 DFEGRDVRGVLLKDLLNENAELGAT-NILDFYPILGGLDIQGIRKKLKEIFRRIPTTWED 259
Query: 772 TVKE 783
+KE
Sbjct: 260 ILKE 263
>ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
[Vitis vinifera]
Length = 515
Score = 160 bits (404), Expect = 8e-37
Identities = 98/244 (40%), Positives = 141/244 (57%), Gaps = 14/244 (5%)
Frame = +1
Query: 94 FAIVVLYLLFKPKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLG 273
F +++ ++ KP LPPGP P WP+IGNL LG N HV L +LA+ HGPLM + LG
Sbjct: 24 FLLILKHIFLKPPP-LPPGPYP-WPIIGNLLQ-LGKNP--HVKLASLAKLHGPLMSLRLG 78
Query: 274 TQPXXXXXXXXXX-----------SGRHIAKSFRIKE--MNKHSLVWAN-CTDNWKLLRK 411
TQ SGR+++ S +K+ +N SL +A CT+NWK LR
Sbjct: 79 TQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRT 138
Query: 412 LARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXXXXXX 591
+ RTE+FS + ++ +RE KV ELVEFL +EG+VVK+ VF T+ NI+ N
Sbjct: 139 ICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSN---TFF 195
Query: 592 XXXXXXXXRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLWEP 771
R G++ I +GA+PNL++FYPIL+GL+L G K++ + +G++ WE
Sbjct: 196 SMDLCDFEREGLKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALGRILATWEG 255
Query: 772 TVKE 783
T+KE
Sbjct: 256 TLKE 259
>ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 496
Score = 157 bits (396), Expect = 7e-36
Identities = 99/277 (35%), Positives = 148/277 (53%), Gaps = 18/277 (6%)
Frame = +1
Query: 100 IVVLYLLFKPKHN---LPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTL 270
I ++ +FK LPPGP P WP+IGN+ I P H+TL N A+ +GPL+ + L
Sbjct: 19 IFFIFKIFKKSSKITQLPPGPTP-WPVIGNIFQI--GKMP-HITLTNFAKTYGPLISLRL 74
Query: 271 GTQPXXXXXXXXXX-----------SGRHIAKSFRIK--EMNKHSLVW-ANCTDNWKLLR 408
GTQ SGRH+ + R K ++ K SL W + C W+ LR
Sbjct: 75 GTQCLVVGSSPSVAIEIFKTHDRILSGRHVPNAVRSKGSDLEKTSLGWKSECDSGWRYLR 134
Query: 409 KLARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXXXXX 588
L RT++F+ ++L+ Q +RE KV ELVEFLR +EG+VV I + VF T+LN++ N
Sbjct: 135 TLCRTDLFAGKVLESQACLREKKVMELVEFLRSKEGQVVNIGELVFATVLNMLSNVLISK 194
Query: 589 XXXXXXXXXRIG-MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLW 765
++SL++ ++ +G++PN+++FYPIL LDLQGL+++ D V K +W
Sbjct: 195 DMIHLEEETEDSEIKSLVRGIVEVGSAPNISDFYPILGKLDLQGLRKKSTDLVTKFCSIW 254
Query: 766 EPTVKEXXXXXXXXXXXXXXXXXEGESKDMLDVLLDN 876
EP ++E +D L+ LLDN
Sbjct: 255 EPIIEE---------RRNSRENDSSSQQDFLETLLDN 282
>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 156 bits (394), Expect = 1e-35
Identities = 95/246 (38%), Positives = 139/246 (56%), Gaps = 16/246 (6%)
Frame = +1
Query: 94 FAIVVLYLLFKPKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLG 273
F +++ ++ KP LPPGP P WP+IGNL +G N H L NLA+ HGPLM + LG
Sbjct: 24 FLVILKHIFLKPPP-LPPGPYP-WPIIGNLLQ-MGKNP--HAKLANLAKLHGPLMSLRLG 78
Query: 274 TQ-----------PXXXXXXXXXXSGRHIAKSFRIK--EMNKHSLVWA-NCTDNWKLLRK 411
TQ SGR+++ S +K ++N S+V+A +C NWK LR
Sbjct: 79 TQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRA 138
Query: 412 LARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXXXXXX 591
+ R E+FS + ++ Q +RE KV ELVEFL +EG+VVK+ VF T+ NI+ N
Sbjct: 139 ICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMD 198
Query: 592 XXXXXXXXRIG--MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLW 765
R+G ++ LI + GA+PNL+++YPIL GLD+QG+ R+ + ++ W
Sbjct: 199 LCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTW 258
Query: 766 EPTVKE 783
E +KE
Sbjct: 259 EDILKE 264
>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 155 bits (393), Expect = 1e-35
Identities = 95/246 (38%), Positives = 139/246 (56%), Gaps = 16/246 (6%)
Frame = +1
Query: 94 FAIVVLYLLFKPKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLG 273
F +++ ++ KP LPPGP P WP+IGNL +G N H L NLA+ HGPLM + LG
Sbjct: 24 FLVILKHIFLKPPP-LPPGPYP-WPIIGNLLQ-MGKNP--HAKLANLAKLHGPLMSLRLG 78
Query: 274 TQ-----------PXXXXXXXXXXSGRHIAKSFRIK--EMNKHSLVWA-NCTDNWKLLRK 411
TQ SGR+++ S +K ++N S+V+A +C NWK LR
Sbjct: 79 TQLMVVASSPAAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNTNWKNLRA 138
Query: 412 LARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXXXXXX 591
+ R E+FS + ++ Q +RE KV ELVEFL +EG+VVK+ VF T+ NI+ N
Sbjct: 139 ICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMD 198
Query: 592 XXXXXXXXRIG--MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLW 765
R+G ++ LI + GA+PNL+++YPIL GLD+QG+ R+ + ++ W
Sbjct: 199 LCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTW 258
Query: 766 EPTVKE 783
E +KE
Sbjct: 259 EDILKE 264
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
isozyme 2; AltName: Full=Cytochrome P450 80B2
gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 153 bits (387), Expect = 7e-35
Identities = 93/246 (37%), Positives = 142/246 (57%), Gaps = 15/246 (6%)
Frame = +1
Query: 91 SFAIVVLYLL--FKPKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLV 264
SF + + ++L +PK NLPPGPRPS P++GNL L H LA+ +G L +
Sbjct: 12 SFLLFLFFILRDSRPK-NLPPGPRPS-PIVGNL---LQLGDKPHAEFAKLAQKYGELFSL 66
Query: 265 TLGTQPXXXXXXXXXX-----------SGRHIAKSFRIKEMNKHSLVWANCTDNWKLLRK 411
LG+Q SGR++ +SFR+KE ++S+VW+ C DNWKLLRK
Sbjct: 67 KLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRK 126
Query: 412 LARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGN--XXXX 585
+ RTE+F+ +M++ Q +RE K E+V+FLR +EG+VVKI + VFGTL+NI GN
Sbjct: 127 VCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKD 186
Query: 586 XXXXXXXXXXRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLW 765
+ ++ + +LL +G S N A+++PI+ LDL G +R A+ + ++ +W
Sbjct: 187 VFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVW 246
Query: 766 EPTVKE 783
+KE
Sbjct: 247 GVMLKE 252
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 151 bits (382), Expect = 3e-34
Identities = 90/245 (36%), Positives = 138/245 (56%), Gaps = 14/245 (5%)
Frame = +1
Query: 91 SFAIVVLYLLFKPKH-NLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVT 267
SF + + ++L P+ NLPPGPRP P++GNL L LA+ +G L +
Sbjct: 12 SFLLFLFFILRDPRQKNLPPGPRPL-PIVGNL---LQLGDKPRAEFAKLAQKYGQLFSLK 67
Query: 268 LGTQPXXXXXXXXXX-----------SGRHIAKSFRIKEMNKHSLVWANCTDNWKLLRKL 414
LG+Q SGR++ +SFR+KE ++S+VW+ C DNWKLLRK+
Sbjct: 68 LGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKV 127
Query: 415 ARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGN--XXXXX 588
RTE+F+ +M++ Q +RE K E+V+FLR +EG+VVKI + VFGTL+NI GN
Sbjct: 128 CRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSKDV 187
Query: 589 XXXXXXXXXRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLWE 768
+ ++ + +LL +G S N A+++PI+ DL G +R AD + ++ +W
Sbjct: 188 FDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVWG 247
Query: 769 PTVKE 783
+KE
Sbjct: 248 INLKE 252
>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
Full=(S)-N-methylcoclaurine oxidase [C-O
phenol-coupling]; AltName: Full=CYPLXXX; AltName:
Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
cytochrome P-450 CYP80 [Berberis stolonifera]
Length = 487
Score = 150 bits (379), Expect = 6e-34
Identities = 94/276 (34%), Positives = 139/276 (50%), Gaps = 14/276 (5%)
Frame = +1
Query: 91 SFAIVVLYLLFKPKH-NLPPGPRPSWPLIGNLPTILGSNTPLHVTLM-NLARAHGPLMLV 264
S ++ +LLFKPKH NLPP P P+WP++G+LP ++ N+P + M N+A+ +GPL+ +
Sbjct: 11 SLVALLYFLLFKPKHTNLPPSP-PAWPIVGHLPDLISKNSPPFLDYMSNIAQKYGPLIHL 69
Query: 265 TLGTQPXXXXXXXXXX-----------SGRHIAKSFRIKEMNKHSLVWANCTDNWKLLRK 411
G SGR FRIK +S++W++ WK RK
Sbjct: 70 KFGLHSSIFASTKEAAMEVLQTNDKVLSGRQPLPCFRIKPHIDYSILWSDSNSYWKKGRK 129
Query: 412 LARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGN-XXXXX 588
+ TEIFS +MLQ QE RE G LV F+ + G VV++ ++FG LN++G+
Sbjct: 130 ILHTEIFSQKMLQAQEKNRERVAGNLVNFIMTKVGDVVELRSWLFGCALNVLGHVVFSKD 189
Query: 589 XXXXXXXXXRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLWE 768
+GM LI +LM G ++A ++P+L DL GLKR+ ++ + K+WE
Sbjct: 190 VFEYSDQSDEVGMDKLIHGMLMTGGDFDVASYFPVLARFDLHGLKRKMDEQFKLLIKIWE 249
Query: 769 PTVKEXXXXXXXXXXXXXXXXXEGESKDMLDVLLDN 876
V E KDMLDVL+ N
Sbjct: 250 GEV------------LARRANRNPEPKDMLDVLIAN 273
>ref|XP_006358870.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 495
Score = 150 bits (379), Expect = 6e-34
Identities = 99/281 (35%), Positives = 147/281 (52%), Gaps = 20/281 (7%)
Frame = +1
Query: 94 FAIVVLYLLFKPKHN-----LPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLM 258
F + + + LFK + LPPGP P WP+IGN I H+TL N A+ +GPLM
Sbjct: 14 FLLPLTFFLFKVFRSSKITQLPPGPTP-WPVIGN---IFHMGKMPHITLTNFAKIYGPLM 69
Query: 259 LVTLGTQPXXXXXXXXXX-----------SGRHIAKSFRIK--EMNKHSLVW-ANCTDNW 396
+ LGTQ SGRH+ + K +++K S+ W + C + W
Sbjct: 70 SLRLGTQCLVVGSSSSAAIEILKTHDRILSGRHVPNAMPAKRSDLDKISMGWNSECHNEW 129
Query: 397 KLLRKLARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNX 576
+ LR L +TE+FS ++L+ Q +RE KV ELVEFLR +EG+VV I + VF T+LN++ N
Sbjct: 130 RCLRTLCKTELFSGKVLESQAYLREKKVMELVEFLRSKEGQVVNIGELVFATVLNMLSNV 189
Query: 577 XXXXXXXXXXXXXRI-GMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 753
G+++LI+ L+ ++PNL++FYP+L LDLQGL++R + K+
Sbjct: 190 LISKDMVNLEKETEDGGIRNLIRGLVEAVSAPNLSDFYPVLGKLDLQGLRKRARYVMTKI 249
Query: 754 NKLWEPTVKEXXXXXXXXXXXXXXXXXEGESKDMLDVLLDN 876
WEP ++E +D L+ LLDN
Sbjct: 250 RSNWEPILEE---------RRKNKVSGSSSQQDFLEALLDN 281
>ref|XP_004234364.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Solanum lycopersicum]
Length = 486
Score = 149 bits (375), Expect = 2e-33
Identities = 95/278 (34%), Positives = 143/278 (51%), Gaps = 17/278 (6%)
Frame = +1
Query: 94 FAIVVLYLLFKPKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLG 273
F +++ L NLPPGPRP W L+GNL I TP HV+L A+ HGPL+ V LG
Sbjct: 16 FFLLIFKQLVPKSTNLPPGPRP-WHLLGNLLQI--GKTP-HVSLAKFAQIHGPLISVKLG 71
Query: 274 TQPXXXXXXXXXXSGRHIAKS---------------FRIKEMNKHSLVWANCTDN-WKLL 405
TQ S I KS + + +++HS+V+++ N WK L
Sbjct: 72 TQ--LVVVASSPASAAEILKSQDRLLSVRSVPKVATYELSVIDQHSIVFSSDLSNQWKFL 129
Query: 406 RKLARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXXXX 585
R RT +FS + ++ Q +RE KV E++ FLR R+G+ VKIS+ +FGT+LN +GN
Sbjct: 130 RAFCRTHLFSPKAVESQAALREKKVSEMMNFLRSRKGETVKISEILFGTILNTLGNLFFS 189
Query: 586 XXXXXXXXXXRI-GMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKL 762
G++ +I++ + +GA PN++EFYP+ + LDLQGL+++ ++ +
Sbjct: 190 KDLCDLDYETNTSGIKHVIRKFVELGAMPNISEFYPLFDALDLQGLRKQTEIYQNRLVNI 249
Query: 763 WEPTVKEXXXXXXXXXXXXXXXXXEGESKDMLDVLLDN 876
W VKE S D LD ++DN
Sbjct: 250 WSEIVKE------------KRQAISRSSSDFLDAMIDN 275
>ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 486
Score = 147 bits (372), Expect = 4e-33
Identities = 91/277 (32%), Positives = 147/277 (53%), Gaps = 15/277 (5%)
Frame = +1
Query: 91 SFAIVVLYLLFKPKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTL 270
+F +++L L NLPPGPRP WPL+GN+ I +P H++L A+ +GPL+ V L
Sbjct: 15 AFFLLILKQLIPKSPNLPPGPRP-WPLLGNILQI--GKSP-HISLAQFAKIYGPLISVKL 70
Query: 271 GTQ-----------PXXXXXXXXXXSGRHIAK--SFRIKEMNKHSLVWANCTDN-WKLLR 408
GTQ S R + K ++ + +++HS+V+++ N WK LR
Sbjct: 71 GTQLVVVASSPDSAAEILKTQDRLLSVRSVPKVATYELSVIDQHSIVFSSDLSNHWKFLR 130
Query: 409 KLARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXXXXX 588
RT +FS + ++ Q +RE KV E++ FLR R+G+ VKIS+ +F T+LN +GN
Sbjct: 131 AFCRTHLFSPKAVESQAALREKKVSEMIHFLRSRKGETVKISEIMFATILNTLGNLFFSK 190
Query: 589 XXXXXXXXXRI-GMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLW 765
G++ +I++ + +GA PN++EFYP+ + LDLQGL+++ ++ +W
Sbjct: 191 DLCDLDYETNTSGIKHVIRKFVELGAMPNISEFYPLFDALDLQGLRKQTEIYQNQLFNIW 250
Query: 766 EPTVKEXXXXXXXXXXXXXXXXXEGESKDMLDVLLDN 876
VKE S D LDV++D+
Sbjct: 251 SDIVKE------------KRQAISRSSSDFLDVMIDS 275
>ref|XP_002318833.1| hypothetical protein POPTR_0012s13580g [Populus trichocarpa]
gi|222859506|gb|EEE97053.1| hypothetical protein
POPTR_0012s13580g [Populus trichocarpa]
Length = 503
Score = 146 bits (369), Expect = 9e-33
Identities = 94/261 (36%), Positives = 136/261 (52%), Gaps = 15/261 (5%)
Frame = +1
Query: 139 LPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLGTQ-----------PX 285
LPPGP+P WP+IGNL + N P HV+L +A+ HGPL+ + LGTQ
Sbjct: 44 LPPGPKP-WPIIGNLLHV--GNQP-HVSLAEIAKIHGPLISLRLGTQLLVVGSSAKAAAE 99
Query: 286 XXXXXXXXXSGRHIAKSFRIKE--MNKHSLVWA-NCTDNWKLLRKLARTEIFSVRMLQIQ 456
S RH+ + + + + +LVW D WKLLR L RTE+FS + ++
Sbjct: 100 ILKTHDRFLSARHVPQVIPRESHVLRRVALVWCPESIDTWKLLRGLCRTELFSAKAIESS 159
Query: 457 EPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGN-XXXXXXXXXXXXXXRIGMQS 633
+RE KVGEL++FL REGKVV I + VF T+ N I N G++S
Sbjct: 160 ATLREKKVGELMDFLVAREGKVVSIGEVVFSTVFNTISNLLFSNDLAGLEEKGMSSGLKS 219
Query: 634 LIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLWEPTVKEXXXXXXXXXX 813
+++L+++ A+PN+A+FYPI GLD QGL+R+ + V + +W +KE
Sbjct: 220 HVRKLMLLVATPNIADFYPIFAGLDPQGLRRKLSKLVEETFAIWAINIKE---------- 269
Query: 814 XXXXXXXEGESKDMLDVLLDN 876
+ +D LDV L N
Sbjct: 270 RRNSYVHDSPKRDFLDVFLAN 290
>ref|XP_006358915.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 495
Score = 146 bits (368), Expect = 1e-32
Identities = 96/276 (34%), Positives = 144/276 (52%), Gaps = 17/276 (6%)
Frame = +1
Query: 100 IVVLYLLFKPKH--NLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLG 273
I ++ +FK LPPGP P WP+IGN I H+TL N A+ +GPLM + LG
Sbjct: 19 IFFIFKIFKSSKITQLPPGPTP-WPVIGN---IFQMGKMPHITLTNFAKTYGPLMSLKLG 74
Query: 274 TQ-----------PXXXXXXXXXXSGRHIAKSFRIK--EMNKHSLVW-ANCTDNWKLLRK 411
T+ SGR + + K +++K SL W + C W+ LR
Sbjct: 75 TKWLVVGSSPLAAVEILKTHDKILSGRDVPSAAPSKGSDLDKTSLGWISECNSGWRYLRT 134
Query: 412 LARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXXXXXX 591
L RTE+F+ ++L+ Q +RE KV ELVEFLR +EG+VV I + VF T+LN++ N
Sbjct: 135 LCRTELFAGKVLESQACLREKKVMELVEFLRSKEGQVVNIGELVFATVLNMLSNVLISKD 194
Query: 592 XXXXXXXXRIG-MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLWE 768
G ++SL++ ++ + ++PN+++ YPIL LDLQGL+++ D V K+ WE
Sbjct: 195 MVNLEEEIEDGVIKSLVRGIVEVLSAPNISDLYPILGKLDLQGLQKKSIDLVTKLCSNWE 254
Query: 769 PTVKEXXXXXXXXXXXXXXXXXEGESKDMLDVLLDN 876
P ++E +D L+ LLDN
Sbjct: 255 PILEE---------RRNSREKGSSSQQDFLETLLDN 281
>ref|XP_006353349.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 487
Score = 145 bits (366), Expect = 2e-32
Identities = 90/279 (32%), Positives = 144/279 (51%), Gaps = 18/279 (6%)
Frame = +1
Query: 94 FAIVVLYLLFKPKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLG 273
F +++ L NLPPGPRP WP++GN+ LG N H++L A HGPL+ V LG
Sbjct: 16 FFFLIVKQLIPKSGNLPPGPRP-WPVLGNILQ-LGKNP--HISLAKFAEIHGPLISVKLG 71
Query: 274 TQPXXXXXXXXXXSGRHIAKS---------------FRIKEMNKHSLVWANCTDN-WKLL 405
TQ S I K+ + + +++HS+V+++ N WK L
Sbjct: 72 TQ--LVVVASSPHSAAEILKTQDRLLSARSPPSVVPYELSVIDQHSIVFSSDLGNHWKFL 129
Query: 406 RKLARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXXXX 585
R RT +FS + ++ Q +R+ KV E++ FLR ++G+ VKIS+ +FGT+LN +GN
Sbjct: 130 RAFCRTHLFSPKAVESQAALRDKKVSEMIHFLRSKKGETVKISEILFGTILNTLGNLFFS 189
Query: 586 XXXXXXXXXXR--IGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNK 759
R G++ + ++ + +GA PN+++FYP+ E LDLQGL+++ ++
Sbjct: 190 KDLVCGLDYERNPSGIKRVFRKYIELGAMPNISDFYPLFEALDLQGLRKQTEIYQSRLIN 249
Query: 760 LWEPTVKEXXXXXXXXXXXXXXXXXEGESKDMLDVLLDN 876
+W VKE S D LDV++D+
Sbjct: 250 IWSEIVKE------------KRQAISRHSSDFLDVMIDS 276
>ref|XP_004234365.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Solanum lycopersicum]
Length = 486
Score = 145 bits (365), Expect = 3e-32
Identities = 96/280 (34%), Positives = 146/280 (52%), Gaps = 21/280 (7%)
Frame = +1
Query: 100 IVVLYLLFK---PKH-NLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVT 267
I +L+L+ K PK NLPPGPR SWPL+GN+ I G N H++L A+ HGPL+ V
Sbjct: 14 IPLLFLIVKQFIPKSGNLPPGPR-SWPLLGNILQI-GKNP--HISLAKFAQIHGPLISVK 69
Query: 268 LGTQPXXXXXXXXXXSGRHIAKS---------------FRIKEMNKHSLVWANCTDN-WK 399
LGTQ S I K+ + + +N+HS+V+++ N WK
Sbjct: 70 LGTQ--LVVVASSPASAAEILKTQDRLLSARSPPSVAPYELSVINQHSIVFSSDLSNHWK 127
Query: 400 LLRKLARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXX 579
LLR RT +FS + ++ Q +RE KV E++ FL+ ++G+ V IS+ +FGT+LN GN
Sbjct: 128 LLRAFCRTHLFSPKAVESQTALREKKVSEMIHFLKSKKGETVMISEILFGTILNTFGNLF 187
Query: 580 XXXXXXXXXXXXRIG-MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVN 756
+ + + ++ + +GA PN++EFYP+LE LDLQGL ++ ++
Sbjct: 188 FSKDLCDLDHETKTSETKRVFRKFIELGAMPNISEFYPLLEALDLQGLSKQTKMYQNQLI 247
Query: 757 KLWEPTVKEXXXXXXXXXXXXXXXXXEGESKDMLDVLLDN 876
+W VKE S D LDV++D+
Sbjct: 248 NIWSQIVKE------------KRQAVNRRSSDFLDVMIDS 275
>ref|XP_006353351.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 491
Score = 144 bits (362), Expect = 6e-32
Identities = 89/279 (31%), Positives = 145/279 (51%), Gaps = 17/279 (6%)
Frame = +1
Query: 91 SFAIVVLYLLFKPKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTL 270
SF ++L L + NLPPGPRP WP++G + +G N H++L A+ +GPL+ V L
Sbjct: 20 SFFFLILKQLTQKSRNLPPGPRP-WPVLGKILQ-MGKNP--HISLAQFAKMYGPLISVKL 75
Query: 271 GTQPXXXXXXXXXXSGRHIAKS---------------FRIKEMNKHSLVWANCTDN-WKL 402
GTQ S I K+ + + +++HS+V+++ N WK
Sbjct: 76 GTQ--LVVVASSPASAAEILKTQDRLLSVRSVPKVAIYELSVIDQHSIVFSSDLSNHWKF 133
Query: 403 LRKLARTEIFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGNXXX 582
LR RT +FS + ++ Q +RE K+ E++EFLR R+G+ VKIS+ +FGT+LN +GN
Sbjct: 134 LRAFCRTHLFSPKAVESQTTLREKKMWEMIEFLRSRKGETVKISEILFGTILNTLGNLFF 193
Query: 583 XXXXXXXXXXXRIG-MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNK 759
++ + ++ + +GA PN+++FYP+ E LDLQGL+++ ++
Sbjct: 194 SKDLCHLDYERNTSEIKCVFRKFIELGAMPNISDFYPLFEALDLQGLRKQTEIYQSRLIN 253
Query: 760 LWEPTVKEXXXXXXXXXXXXXXXXXEGESKDMLDVLLDN 876
+W VKE S D LDV++D+
Sbjct: 254 IWSEIVKE------------KRHAISRHSSDFLDVMIDS 280
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 142 bits (358), Expect = 2e-31
Identities = 85/241 (35%), Positives = 131/241 (54%), Gaps = 16/241 (6%)
Frame = +1
Query: 109 LYLLFK---PKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLGTQ 279
LYL+F+ PK LPPGP+P WP++GNL L H LA+ +G L + LG++
Sbjct: 16 LYLIFRDSSPK-GLPPGPKP-WPIVGNL---LQLGEKPHSQFAELAKTYGDLFTLKLGSE 70
Query: 280 PXXXXXXXXXXS-----------GRHIAKSFRIKEMNKHSLVWANCTDNWKLLRKLARTE 426
S GR++ +SFR+KE ++S+VW+ C + WK LRK+ R E
Sbjct: 71 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRAE 130
Query: 427 IFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGN--XXXXXXXXX 600
+F+ +M++ Q +RE+K E+VEFL+R +G VKI + VFGTL+NI GN
Sbjct: 131 LFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLG 190
Query: 601 XXXXXRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLWEPTVK 780
+ M+ + +L +G S N A+++P L DL G ++ AD + + +W +K
Sbjct: 191 DESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLK 250
Query: 781 E 783
E
Sbjct: 251 E 251
>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 140 bits (352), Expect = 8e-31
Identities = 85/241 (35%), Positives = 130/241 (53%), Gaps = 16/241 (6%)
Frame = +1
Query: 109 LYLLFK---PKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLGTQ 279
LYL+F+ PK LPPGP+P WP++GNL L H LA +G L + LG++
Sbjct: 11 LYLIFRDSSPK-GLPPGPKP-WPIVGNL---LQLGEKPHSQFAQLAETYGDLFSLKLGSE 65
Query: 280 PXXXXXXXXXXS-----------GRHIAKSFRIKEMNKHSLVWANCTDNWKLLRKLARTE 426
S GR++ +SFR+KE ++S+VW+ C + WK LRK+ RTE
Sbjct: 66 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 125
Query: 427 IFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGN--XXXXXXXXX 600
+F+ +M++ Q VRE+K E+VE+L++ G VKI++ VFGTL+NI GN
Sbjct: 126 LFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLG 185
Query: 601 XXXXXRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLWEPTVK 780
+ M+ + +L +G S N A+++P L DL G + AD + + +W +K
Sbjct: 186 DESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLK 245
Query: 781 E 783
E
Sbjct: 246 E 246
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 139 bits (349), Expect = 2e-30
Identities = 84/241 (34%), Positives = 130/241 (53%), Gaps = 16/241 (6%)
Frame = +1
Query: 109 LYLLFK---PKHNLPPGPRPSWPLIGNLPTILGSNTPLHVTLMNLARAHGPLMLVTLGTQ 279
LYL+F+ PK LPPGP+P WP++GNL L H LA +G L + LG++
Sbjct: 11 LYLIFRDSSPK-GLPPGPKP-WPIVGNL---LQLGEKPHSQFAQLAETYGDLFSLKLGSE 65
Query: 280 PXXXXXXXXXXS-----------GRHIAKSFRIKEMNKHSLVWANCTDNWKLLRKLARTE 426
S GR++ +SFR+KE ++S+VW+ C + WK LRK+ RT+
Sbjct: 66 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTD 125
Query: 427 IFSVRMLQIQEPVRENKVGELVEFLRRREGKVVKISKFVFGTLLNIIGN--XXXXXXXXX 600
+F+ +M++ Q VRE+K E+VE+L++ G VKI++ VFGTL+NI GN
Sbjct: 126 LFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLG 185
Query: 601 XXXXXRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLWEPTVK 780
+ M+ + +L +G S N A+++P L DL G + AD + + +W +K
Sbjct: 186 DESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLK 245
Query: 781 E 783
E
Sbjct: 246 E 246