BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cnidium21_contig00030577
         (603 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q43469.1|SLD1_HELAN RecName: Full=Delta(8)-fatty-acid desatur...   291   5e-77
gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]    287   1e-75
gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]        280   2e-73
gb|ADA60229.1| delta-8 sphingolipid desaturase [Ribes nigrum]         253   2e-65
sp|Q9FR82.1|SLD1_BOROF RecName: Full=Delta(8)-fatty-acid desatur...   252   4e-65

>sp|Q43469.1|SLD1_HELAN RecName: Full=Delta(8)-fatty-acid desaturase; AltName:
           Full=Delta(8)-sphingolipid desaturase
           gi|1040729|emb|CAA60621.1| delta-8 sphingolipid
           desaturase [Helianthus annuus]
          Length = 458

 Score =  291 bits (746), Expect = 5e-77
 Identities = 134/177 (75%), Positives = 154/177 (87%)
 Frame = -2

Query: 533 PLTGALGSMVTAKKYITSEELGKHNKREDLWISIQGKVYNVTEWAKEHPGGDIPLLNLAG 354
           P    L S+   KKYITS+EL KHN   DLWISI GKVYNVTEWAKEHPGGD PL+NLAG
Sbjct: 4   PSIEVLNSIADGKKYITSKELKKHNNPNDLWISILGKVYNVTEWAKEHPGGDAPLINLAG 63

Query: 353 QDVTDAFIAFHPGSAWKYLDKFFTGYHLIDYEISDVSKDYRKLAAEFAKAGMFEKKGHIV 174
           QDVTDAFIAFHPG+AWK+LDK FTGYHL DY++SD+S+DYRKLA+EFAKAGMFEKKGH V
Sbjct: 64  QDVTDAFIAFHPGTAWKHLDKLFTGYHLKDYQVSDISRDYRKLASEFAKAGMFEKKGHGV 123

Query: 173 VYSICFVALLLSACVYGVLGSDKFSVHMLSGALLGFAWIQVAYMGHDAGHYQAMSSR 3
           +YS+CFV+LLLSACVYGVL S  F +HMLSGA+LG AW+Q+AY+GHDAGHYQ M++R
Sbjct: 124 IYSLCFVSLLLSACVYGVLYSGSFWIHMLSGAILGLAWMQIAYLGHDAGHYQMMATR 180


>gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]
          Length = 466

 Score =  287 bits (734), Expect = 1e-75
 Identities = 134/165 (81%), Positives = 149/165 (90%)
 Frame = -2

Query: 497 KKYITSEELGKHNKREDLWISIQGKVYNVTEWAKEHPGGDIPLLNLAGQDVTDAFIAFHP 318
           KKYITS+EL KHNK  DLWISIQGKVYNVTEWAK HPGG+IPL+NLAGQDVTDAFIAFHP
Sbjct: 24  KKYITSDELKKHNKPNDLWISIQGKVYNVTEWAKIHPGGEIPLMNLAGQDVTDAFIAFHP 83

Query: 317 GSAWKYLDKFFTGYHLIDYEISDVSKDYRKLAAEFAKAGMFEKKGHIVVYSICFVALLLS 138
           GSAW++L K FTGYHL DY++S VSKDYRKLA+EFAKAGMFEKKGH V+YS+CFV+LLLS
Sbjct: 84  GSAWEHLSKLFTGYHLKDYQVSAVSKDYRKLASEFAKAGMFEKKGHGVIYSLCFVSLLLS 143

Query: 137 ACVYGVLGSDKFSVHMLSGALLGFAWIQVAYMGHDAGHYQAMSSR 3
            CVYGVL S  F VHML+GALLG AWIQ+AY+GHDAGHYQ MS+R
Sbjct: 144 GCVYGVLFSSSFWVHMLAGALLGLAWIQIAYLGHDAGHYQMMSTR 188


>gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]
          Length = 469

 Score =  280 bits (715), Expect = 2e-73
 Identities = 128/165 (77%), Positives = 147/165 (89%)
 Frame = -2

Query: 497 KKYITSEELGKHNKREDLWISIQGKVYNVTEWAKEHPGGDIPLLNLAGQDVTDAFIAFHP 318
           KKYITSEEL KHNK  DLWISIQGKVYNVTEW+K HPGGDIPLLNLAGQDVTDAFIAFHP
Sbjct: 27  KKYITSEELEKHNKPTDLWISIQGKVYNVTEWSKIHPGGDIPLLNLAGQDVTDAFIAFHP 86

Query: 317 GSAWKYLDKFFTGYHLIDYEISDVSKDYRKLAAEFAKAGMFEKKGHIVVYSICFVALLLS 138
           GSAW +L   FTGYHL DY++SD+SKDYRKLA+EF+KAGMF+KKGH V+YS+CFV++LLS
Sbjct: 87  GSAWHHLQSLFTGYHLQDYQVSDISKDYRKLASEFSKAGMFDKKGHGVIYSLCFVSILLS 146

Query: 137 ACVYGVLGSDKFSVHMLSGALLGFAWIQVAYMGHDAGHYQAMSSR 3
            CVYGVL S   +VHM +GALLG AW+Q+AY+GHDAGHYQ M++R
Sbjct: 147 LCVYGVLCSASVTVHMFAGALLGLAWMQIAYLGHDAGHYQMMATR 191


>gb|ADA60229.1| delta-8 sphingolipid desaturase [Ribes nigrum]
          Length = 448

 Score =  253 bits (646), Expect = 2e-65
 Identities = 116/164 (70%), Positives = 136/164 (82%)
 Frame = -2

Query: 497 KKYITSEELGKHNKREDLWISIQGKVYNVTEWAKEHPGGDIPLLNLAGQDVTDAFIAFHP 318
           KKY+T EEL +HNK EDLWISIQGKVYNVT W K+HPGGDIPLLNL GQDVTDAFIA+HP
Sbjct: 6   KKYMTVEELKEHNKPEDLWISIQGKVYNVTVWKKQHPGGDIPLLNLGGQDVTDAFIAYHP 65

Query: 317 GSAWKYLDKFFTGYHLIDYEISDVSKDYRKLAAEFAKAGMFEKKGHIVVYSICFVALLLS 138
           G+AW+YLD+FFTGY+L D+++SDVSKDYRKLA EF K G+FE KGH V+Y+ C VA L +
Sbjct: 66  GTAWQYLDRFFTGYYLKDFKVSDVSKDYRKLAYEFNKMGLFENKGHGVLYAFCLVAFLFA 125

Query: 137 ACVYGVLGSDKFSVHMLSGALLGFAWIQVAYMGHDAGHYQAMSS 6
             VYGVL S+   VHM  G LLGF W+Q AY+GHD+GHYQ MS+
Sbjct: 126 VVVYGVLCSESVFVHMCCGGLLGFLWMQSAYVGHDSGHYQTMST 169


>sp|Q9FR82.1|SLD1_BOROF RecName: Full=Delta(8)-fatty-acid desaturase; AltName:
           Full=Delta(8)-sphingolipid desaturase
           gi|12002282|gb|AAG43277.1|AF133728_1 delta
           8-sphingolipid desaturase [Borago officinalis]
          Length = 446

 Score =  252 bits (643), Expect = 4e-65
 Identities = 114/169 (67%), Positives = 140/169 (82%)
 Frame = -2

Query: 509 MVTAKKYITSEELGKHNKREDLWISIQGKVYNVTEWAKEHPGGDIPLLNLAGQDVTDAFI 330
           M   KKYI+  EL KHN+  D+WISIQGKVYNVT+W K+HPGGD+P++NLAGQD TDAFI
Sbjct: 1   MEGTKKYISVGELEKHNQLGDVWISIQGKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFI 60

Query: 329 AFHPGSAWKYLDKFFTGYHLIDYEISDVSKDYRKLAAEFAKAGMFEKKGHIVVYSICFVA 150
           A+HPG+AWK L+  FTGYHL DY +S++SKDYRKLA+EF+KAG+FEKKGH V+Y + F+A
Sbjct: 61  AYHPGTAWKNLENLFTGYHLEDYLVSEISKDYRKLASEFSKAGLFEKKGHTVIYCLSFIA 120

Query: 149 LLLSACVYGVLGSDKFSVHMLSGALLGFAWIQVAYMGHDAGHYQAMSSR 3
           LLL  CVYGVL S+   VHMLSGA+LG  +IQ AY+GHD+GHY  MSS+
Sbjct: 121 LLLCGCVYGVLCSNSLWVHMLSGAMLGMCFIQAAYLGHDSGHYTMMSSK 169