BLASTX nr result
ID: Cnidium21_contig00028820
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cnidium21_contig00028820
(306 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_001767715.1| predicted protein [Physcomitrella patens sub... 76 3e-12
ref|XP_002274578.2| PREDICTED: TMV resistance protein N-like [Vi... 74 1e-11
emb|CAR94518.1| nematode resistance-like protein [Prunus cerasif... 72 4e-11
emb|CAR94514.1| nematode resistance-like protein [Prunus cerasif... 72 4e-11
ref|XP_002272079.2| PREDICTED: TMV resistance protein N-like [Vi... 72 4e-11
>ref|XP_001767715.1| predicted protein [Physcomitrella patens subsp. patens]
gi|162681035|gb|EDQ67466.1| predicted protein
[Physcomitrella patens subsp. patens]
Length = 539
Score = 75.9 bits (185), Expect = 3e-12
Identities = 42/100 (42%), Positives = 63/100 (63%), Gaps = 1/100 (1%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDA-SYTAI 127
L VL+L C +K L E + ++L LYLG C +L+ LP+ +G + +LK+L+ ++
Sbjct: 439 LKVLNLIGCGSLKTLPESMGNLNSLVELYLGECGSLKVLPESMGNLNFLKKLNLYGCGSL 498
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGLRTF 7
E LP S+ L LV L+L GCK L+ LPE IGN++ L+ F
Sbjct: 499 EALPKSMGNLNSLVELDLRGCKTLEALPESIGNLKNLKVF 538
Score = 69.7 bits (169), Expect = 2e-10
Identities = 39/98 (39%), Positives = 57/98 (58%), Gaps = 1/98 (1%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELD-ASYTAI 127
L LDL EC +K L E + ++L L L C +L+ LP+ +G + L ELD ++
Sbjct: 343 LVELDLGECGSLKALPESMGNLNSLVQLNLSKCGSLKALPESMGNLNSLVELDLGGCESL 402
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGLR 13
E LP+S++ L LV L L GC LK LP+ +GN+ L+
Sbjct: 403 EALPESMSNLNSLVKLYLYGCGSLKALPKSMGNLNSLK 440
Score = 67.8 bits (164), Expect = 9e-10
Identities = 37/97 (38%), Positives = 58/97 (59%), Gaps = 1/97 (1%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYT-AI 127
L LDL EC +K L E + ++L L L C +L+ LP+ +G + L +L+ S ++
Sbjct: 7 LVELDLGECRSLKALPESMGNLNSLVQLNLSRCGSLKALPESMGNLNSLVQLNLSRCGSL 66
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGL 16
+ LP+S+ L LV L+L GC+ L+ LPE +GN+ L
Sbjct: 67 KALPESMGNLNSLVELDLGGCESLEALPESMGNLNSL 103
Score = 64.3 bits (155), Expect = 1e-08
Identities = 39/97 (40%), Positives = 54/97 (55%), Gaps = 1/97 (1%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTA-I 127
L LDLN C +K L E + ++L L L C +L+ LP+ +G L EL +
Sbjct: 103 LLKLDLNVCRSLKALPESMSNLNSLVKLNLYECGSLKTLPESMGNWNSLVELFLYGCGFL 162
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGL 16
+ LP+S+ LK LV LNL GC L+ LPE +GN+ L
Sbjct: 163 KALPESMGNLKSLVQLNLIGCGSLEALPESMGNLNSL 199
Score = 63.2 bits (152), Expect = 2e-08
Identities = 35/97 (36%), Positives = 56/97 (57%), Gaps = 1/97 (1%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELD-ASYTAI 127
L L+L+ C +K L E + ++L L L C +L+ LP+ +G + L ELD ++
Sbjct: 31 LVQLNLSRCGSLKALPESMGNLNSLVQLNLSRCGSLKALPESMGNLNSLVELDLGGCESL 90
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGL 16
E LP+S+ L L+ L+L+ C+ LK LPE + N+ L
Sbjct: 91 EALPESMGNLNSLLKLDLNVCRSLKALPESMSNLNSL 127
Score = 62.0 bits (149), Expect = 5e-08
Identities = 35/101 (34%), Positives = 59/101 (58%), Gaps = 1/101 (0%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYT-AI 127
L L+L C ++ L E + ++L L LG C +L+ LP+ +G + L +L+ S ++
Sbjct: 175 LVQLNLIGCGSLEALPESMGNLNSLVELDLGECRSLKALPESMGNLNSLVQLNLSRCGSL 234
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGLRTFY 4
+ P+S+ L LV L+L+GC+ L+ LPE +GN+ L Y
Sbjct: 235 KAFPESMGNLNSLVQLDLEGCESLEALPESMGNLNSLVGLY 275
Score = 62.0 bits (149), Expect = 5e-08
Identities = 36/101 (35%), Positives = 58/101 (57%), Gaps = 1/101 (0%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYT-AI 127
L L+L C +K L E + ++L L LG C +L+ LP+ +G + L +L+ S ++
Sbjct: 319 LVKLNLIGCGSLKALLESMGNLNSLVELDLGECGSLKALPESMGNLNSLVQLNLSKCGSL 378
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGLRTFY 4
+ LP+S+ L LV L+L GC+ L+ LPE + N+ L Y
Sbjct: 379 KALPESMGNLNSLVELDLGGCESLEALPESMSNLNSLVKLY 419
Score = 61.6 bits (148), Expect = 6e-08
Identities = 37/98 (37%), Positives = 55/98 (56%), Gaps = 1/98 (1%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELD-ASYTAI 127
L LDL C ++ L E + ++L LYL C +L+ LPK +G + LK L+ ++
Sbjct: 391 LVELDLGGCESLEALPESMSNLNSLVKLYLYGCGSLKALPKSMGNLNSLKVLNLIGCGSL 450
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGLR 13
+ LP+S+ L LV L L C LK LPE +GN+ L+
Sbjct: 451 KTLPESMGNLNSLVELYLGECGSLKVLPESMGNLNFLK 488
Score = 60.8 bits (146), Expect = 1e-07
Identities = 36/97 (37%), Positives = 56/97 (57%), Gaps = 1/97 (1%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYT-AI 127
L LDL C ++ L E + ++L LY+ C +L+ LP+ +G + L +L+ S ++
Sbjct: 247 LVQLDLEGCESLEALPESMGNLNSLVGLYVIECRSLKALPESMGNLNSLVQLNLSRCGSL 306
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGL 16
+ LP+S+ L LV LNL GC LK L E +GN+ L
Sbjct: 307 KALPESMGNLNSLVKLNLIGCGSLKALLESMGNLNSL 343
Score = 59.7 bits (143), Expect = 2e-07
Identities = 36/97 (37%), Positives = 51/97 (52%), Gaps = 1/97 (1%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELD-ASYTAI 127
L L L C ++K L E + +L L L C +L LP+ +G + L ELD ++
Sbjct: 151 LVELFLYGCGFLKALPESMGNLKSLVQLNLIGCGSLEALPESMGNLNSLVELDLGECRSL 210
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGL 16
+ LP+S+ L LV LNL C LK PE +GN+ L
Sbjct: 211 KALPESMGNLNSLVQLNLSRCGSLKAFPESMGNLNSL 247
Score = 57.8 bits (138), Expect = 9e-07
Identities = 40/121 (33%), Positives = 55/121 (45%), Gaps = 25/121 (20%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDA----SY 136
L LDL EC +K L E + ++L L L C +L+ P+ +G + L +LD S
Sbjct: 199 LVELDLGECRSLKALPESMGNLNSLVQLNLSRCGSLKAFPESMGNLNSLVQLDLEGCESL 258
Query: 135 TAIEE---------------------LPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEG 19
A+ E LP+S+ L LV LNL C LK LPE +GN+
Sbjct: 259 EALPESMGNLNSLVGLYVIECRSLKALPESMGNLNSLVQLNLSRCGSLKALPESMGNLNS 318
Query: 18 L 16
L
Sbjct: 319 L 319
Score = 55.5 bits (132), Expect = 4e-06
Identities = 30/75 (40%), Positives = 46/75 (61%), Gaps = 1/75 (1%)
Frame = -1
Query: 237 STLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYT-AIEELPDSITQLKELVYLNLDGCK 61
++L L LG C +L+ LP+ +G + L +L+ S +++ LP+S+ L LV LNL C
Sbjct: 5 NSLVELDLGECRSLKALPESMGNLNSLVQLNLSRCGSLKALPESMGNLNSLVQLNLSRCG 64
Query: 60 KLKKLPEQIGNMEGL 16
LK LPE +GN+ L
Sbjct: 65 SLKALPESMGNLNSL 79
>ref|XP_002274578.2| PREDICTED: TMV resistance protein N-like [Vitis vinifera]
Length = 1535
Score = 73.9 bits (180), Expect = 1e-11
Identities = 38/85 (44%), Positives = 53/85 (62%)
Frame = -1
Query: 270 VKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAIEELPDSITQLKE 91
+K L + + +L +L L CS + P++ G MK L ELD YTAI++LPDSI L+
Sbjct: 882 IKDLPDSIGDLESLMFLNLSGCSKFEKFPEKGGNMKSLMELDLRYTAIKDLPDSIGDLES 941
Query: 90 LVYLNLDGCKKLKKLPEQIGNMEGL 16
L L+L GC K +K PE+ GNM+ L
Sbjct: 942 LRLLDLSGCSKFEKFPEKGGNMKSL 966
Score = 72.4 bits (176), Expect = 4e-11
Identities = 41/98 (41%), Positives = 57/98 (58%)
Frame = -1
Query: 306 RLFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAI 127
+L L L C +K L + + +L L L CS + P++ G MK L +LD +TAI
Sbjct: 776 KLTTLSLRFCDQLKNLPDSIGYLESLESLDLSDCSKFVKFPEKGGNMKSLMKLDLRFTAI 835
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGLR 13
++LPDSI L+ L LNL C K +K PE+ GNM+ LR
Sbjct: 836 KDLPDSIGDLESLESLNLSFCSKFEKFPEKGGNMKSLR 873
Score = 72.0 bits (175), Expect = 5e-11
Identities = 36/85 (42%), Positives = 53/85 (62%)
Frame = -1
Query: 270 VKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAIEELPDSITQLKE 91
+K L + + +L L L +CS + P++ G MK L+ L TAI++LPDSI L+
Sbjct: 835 IKDLPDSIGDLESLESLNLSFCSKFEKFPEKGGNMKSLRHLCLRNTAIKDLPDSIGDLES 894
Query: 90 LVYLNLDGCKKLKKLPEQIGNMEGL 16
L++LNL GC K +K PE+ GNM+ L
Sbjct: 895 LMFLNLSGCSKFEKFPEKGGNMKSL 919
Score = 70.5 bits (171), Expect = 1e-10
Identities = 36/89 (40%), Positives = 54/89 (60%)
Frame = -1
Query: 270 VKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAIEELPDSITQLKE 91
+K L + + +L L+L CS + P++ G MK L +LD YTAI++LPDSI L+
Sbjct: 1023 IKDLPDSIGDLESLLSLHLSDCSKFEKFPEKGGNMKSLMKLDLRYTAIKDLPDSIGDLES 1082
Query: 90 LVYLNLDGCKKLKKLPEQIGNMEGLRTFY 4
L L+L C K +K PE+ GNM+ L+ +
Sbjct: 1083 LRLLDLSDCSKFEKFPEKGGNMKSLKKLF 1111
Score = 68.9 bits (167), Expect = 4e-10
Identities = 37/89 (41%), Positives = 52/89 (58%)
Frame = -1
Query: 270 VKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAIEELPDSITQLKE 91
+K L + + +L L L CS + P++ G MK L ELD TAI++LPDSI L+
Sbjct: 929 IKDLPDSIGDLESLRLLDLSGCSKFEKFPEKGGNMKSLVELDLKNTAIKDLPDSIGDLES 988
Query: 90 LVYLNLDGCKKLKKLPEQIGNMEGLRTFY 4
L L+L C K +K PE+ GNM+ L+ Y
Sbjct: 989 LESLDLSDCSKFEKFPEKGGNMKSLKWLY 1017
Score = 65.9 bits (159), Expect = 3e-09
Identities = 35/85 (41%), Positives = 51/85 (60%)
Frame = -1
Query: 270 VKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAIEELPDSITQLKE 91
+K L + + +L L L CS + P++ G MK L +LD + TAI++LPDSI L+
Sbjct: 1117 IKDLPDSIGDLESLESLDLSDCSKFEKFPEKGGNMKSLMDLDLTNTAIKDLPDSIGDLES 1176
Query: 90 LVYLNLDGCKKLKKLPEQIGNMEGL 16
L +L L C K +K PE+ GNM+ L
Sbjct: 1177 LKFLVLSDCSKFEKFPEKGGNMKSL 1201
Score = 63.9 bits (154), Expect = 1e-08
Identities = 39/96 (40%), Positives = 55/96 (57%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAIE 124
L LDL +K L + + +L L L CS + P++ G MK LK L + TAI+
Sbjct: 966 LVELDLKNTA-IKDLPDSIGDLESLESLDLSDCSKFEKFPEKGGNMKSLKWLYLTNTAIK 1024
Query: 123 ELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGL 16
+LPDSI L+ L+ L+L C K +K PE+ GNM+ L
Sbjct: 1025 DLPDSIGDLESLLSLHLSDCSKFEKFPEKGGNMKSL 1060
Score = 63.5 bits (153), Expect = 2e-08
Identities = 35/85 (41%), Positives = 50/85 (58%)
Frame = -1
Query: 270 VKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAIEELPDSITQLKE 91
+K L + + +L L L CS + P++ G MK LK+L TAI++LPDSI L+
Sbjct: 1070 IKDLPDSIGDLESLRLLDLSDCSKFEKFPEKGGNMKSLKKLFLRNTAIKDLPDSIGDLES 1129
Query: 90 LVYLNLDGCKKLKKLPEQIGNMEGL 16
L L+L C K +K PE+ GNM+ L
Sbjct: 1130 LESLDLSDCSKFEKFPEKGGNMKSL 1154
>emb|CAR94518.1| nematode resistance-like protein [Prunus cerasifera]
Length = 2041
Score = 72.4 bits (176), Expect = 4e-11
Identities = 41/88 (46%), Positives = 58/88 (65%), Gaps = 1/88 (1%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSST-LGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAI 127
L +L+L EC + L +++ + T L L L CSNL +LP+ LG +K LK+L AS TAI
Sbjct: 806 LTLLNLRECKNLLTLPDVICTNLTSLQILNLSGCSNLNELPENLGSLKCLKDLYASRTAI 865
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLP 43
++P+SI+QL +L L LDGC L+ LP
Sbjct: 866 SQVPESISQLSQLEELVLDGCSMLQSLP 893
Score = 68.6 bits (166), Expect = 5e-10
Identities = 46/127 (36%), Positives = 66/127 (51%), Gaps = 26/127 (20%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSST-LGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAI 127
L +L+L +C + L +++ S T L L + CSNL +LP+ LG ++ L+EL AS TAI
Sbjct: 734 LTLLNLRDCKNLLSLPDVICTSLTSLQILNVSGCSNLNELPENLGSLECLQELYASRTAI 793
Query: 126 EELPDSI-------------------------TQLKELVYLNLDGCKKLKKLPEQIGNME 22
+ELP SI T L L LNL GC L +LPE +G+++
Sbjct: 794 QELPTSIKHLTDLTLLNLRECKNLLTLPDVICTNLTSLQILNLSGCSNLNELPENLGSLK 853
Query: 21 GLRTFYA 1
L+ YA
Sbjct: 854 CLKDLYA 860
Score = 55.1 bits (131), Expect = 6e-06
Identities = 39/123 (31%), Positives = 57/123 (46%), Gaps = 25/123 (20%)
Frame = -1
Query: 294 LDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAIEELP 115
L L C + + + ++ S ++ G CS L++LP+ MK L++L TAIEELP
Sbjct: 667 LILKGCTSLSAVPDDINLRSLTNFILSG-CSKLKKLPEIGEDMKQLRKLHLDGTAIEELP 725
Query: 114 DSI-------------------------TQLKELVYLNLDGCKKLKKLPEQIGNMEGLRT 10
SI T L L LN+ GC L +LPE +G++E L+
Sbjct: 726 TSIKHLTGLTLLNLRDCKNLLSLPDVICTSLTSLQILNVSGCSNLNELPENLGSLECLQE 785
Query: 9 FYA 1
YA
Sbjct: 786 LYA 788
>emb|CAR94514.1| nematode resistance-like protein [Prunus cerasifera]
Length = 2048
Score = 72.4 bits (176), Expect = 4e-11
Identities = 41/88 (46%), Positives = 59/88 (67%), Gaps = 1/88 (1%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSST-LGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAI 127
L +L+L EC + L +++ + T L L L CSNL +LP+ LG ++ L+EL AS TAI
Sbjct: 806 LTLLNLRECKNLLTLPDVICTNLTSLQILNLSGCSNLNELPENLGSLECLQELYASGTAI 865
Query: 126 EELPDSITQLKELVYLNLDGCKKLKKLP 43
++P+SI+QL +L L LDGC KL+ LP
Sbjct: 866 SQIPESISQLSQLGELVLDGCSKLQSLP 893
Score = 70.5 bits (171), Expect = 1e-10
Identities = 47/127 (37%), Positives = 66/127 (51%), Gaps = 26/127 (20%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSST-LGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAI 127
L +L+L +C + L +++ S T L L + CSNL +LP+ LG ++ L+EL AS TAI
Sbjct: 734 LILLNLRDCKNLLSLPDVICTSLTSLQILNVSGCSNLNELPENLGSLECLQELYASRTAI 793
Query: 126 EELPDSI-------------------------TQLKELVYLNLDGCKKLKKLPEQIGNME 22
+ELP SI T L L LNL GC L +LPE +G++E
Sbjct: 794 QELPTSIKHLTDLTLLNLRECKNLLTLPDVICTNLTSLQILNLSGCSNLNELPENLGSLE 853
Query: 21 GLRTFYA 1
L+ YA
Sbjct: 854 CLQELYA 860
Score = 55.1 bits (131), Expect = 6e-06
Identities = 34/87 (39%), Positives = 48/87 (55%)
Frame = -1
Query: 294 LDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAIEELP 115
L L C + + + ++ S ++ G CS L++LP+ MK L++L TAIEELP
Sbjct: 667 LILKGCTSLSAVPDDINLRSLTNFILSG-CSKLKKLPEIGEDMKQLRKLHLDGTAIEELP 725
Query: 114 DSITQLKELVYLNLDGCKKLKKLPEQI 34
SI L L+ LNL CK L LP+ I
Sbjct: 726 TSIKHLTGLILLNLRDCKNLLSLPDVI 752
>ref|XP_002272079.2| PREDICTED: TMV resistance protein N-like [Vitis vinifera]
gi|296081003|emb|CBI18507.3| unnamed protein product
[Vitis vinifera]
Length = 1281
Score = 72.4 bits (176), Expect = 4e-11
Identities = 42/98 (42%), Positives = 57/98 (58%)
Frame = -1
Query: 303 LFVLDLNECIYVKVLAELLDQSSTLGYLYLGYCSNLRQLPKQLGGMKWLKELDASYTAIE 124
L +LDL C + L + + +L YL+L CS L P+ + M+ LKEL T+IE
Sbjct: 761 LVLLDLKRCKNLTSLPTCIFKLKSLEYLFLSGCSKLENFPEIMEDMENLKELLLDGTSIE 820
Query: 123 ELPDSITQLKELVYLNLDGCKKLKKLPEQIGNMEGLRT 10
LP SI +LK LV LNL CKKL LP+ + N+ L+T
Sbjct: 821 VLPSSIERLKGLVLLNLRKCKKLVSLPDSMCNLRSLQT 858