BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cnidium21_contig00026184
         (1329 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002266572.2| PREDICTED: uncharacterized protein LOC100267...   392   e-106
emb|CBI15293.3| unnamed protein product [Vitis vinifera]              392   e-106
ref|XP_002511501.1| zinc finger protein, putative [Ricinus commu...   325   2e-86
ref|XP_004138295.1| PREDICTED: uncharacterized protein LOC101212...   324   4e-86
ref|XP_003550768.1| PREDICTED: uncharacterized protein LOC100802...   316   7e-84

>ref|XP_002266572.2| PREDICTED: uncharacterized protein LOC100267228 [Vitis vinifera]
          Length = 1288

 Score =  392 bits (1007), Expect = e-106
 Identities = 223/415 (53%), Positives = 265/415 (63%), Gaps = 27/415 (6%)
 Frame = -2

Query: 1166 NGVGLVDNPILVLIYFHKALRAEFAELRRVAVEALESGTHGXXXXXXXXXXXXXXXLF-- 993
            +G  L D PIL+ ++FHKALRAE AELRR+A  A ESG  G                F  
Sbjct: 38   SGAQLRDAPILLFVFFHKALRAELAELRRLA--AAESGCRGGSSNGNLVVEIRRRFEFLK 95

Query: 992  --YKYHSAAEDEVIFLALDELVKNVVSAYSLEHKSIDDLFDSVFNCLDVLDKEGKDNYKT 819
              YKYHSAAEDEVIFLALD  +KNV   YSLEHKSIDDLF S+F+CLDVL +   +  K 
Sbjct: 96   LFYKYHSAAEDEVIFLALDVHIKNVAHTYSLEHKSIDDLFSSIFHCLDVLMEGDANTAKP 155

Query: 818  FQGLLFCIGTIQTTIYQHMLKEEEQVFPLLMQQFSSSEQASLVWQFMCSVPVTLLEDFLP 639
            FQ L+  I TIQTTI  HMLKEEEQVFPLLM+QFS  EQASLVWQFMCSVPV LLEDFLP
Sbjct: 156  FQELVLLISTIQTTICHHMLKEEEQVFPLLMKQFSPQEQASLVWQFMCSVPVLLLEDFLP 215

Query: 638  WMISVLSSDEQIEVRHSLKEIVPKEKLLQEVVISWIDNKVPVFGGCTSIREQA-----AQ 474
            WM S LS +EQ+ V + +KE+VP+EKLL+EVVISW+ N    FG  T I E+A     A 
Sbjct: 216  WMTSFLSPEEQVNVVNCIKEVVPEEKLLEEVVISWLGNNNQPFGSPTRIGEEAQSVGPAN 275

Query: 473  FHTGLGTYHETS------------------RHRSFDGIRPWHDAIRKDLQEVLVELYQIK 348
                L  Y   S                       DG+  WH AIRKDL+ +L ELYQI+
Sbjct: 276  LKDALKVYSSKSFSTEIRQWKEVFGLKTNVGQNPVDGLHLWHGAIRKDLKGILEELYQIR 335

Query: 347  ISNNFSDLPAVVVQLNFIADTLIFYSKALSNIIYPLWNELAKDFYSARYAQYLDERKIEG 168
             S++FS L +V+VQL F+AD LIFYS AL  I YPL + L+    S  Y ++ DE +IEG
Sbjct: 336  SSDSFSTLASVIVQLKFLADVLIFYSNALDKIFYPLLDLLSDGCLSPSYKRFPDESQIEG 395

Query: 167  LQRLLYYKSEKTIPLRNYVEKLYEELGSFASWINENLNLIEAEVFPLIRMNCSHD 3
            LQRLL+Y ++  IPL  +VEKL  EL SF   I ++L   E EVFPLI   CSH+
Sbjct: 396  LQRLLHYNAKNGIPLSKFVEKLCWELESFVMGIGDHLVFQEMEVFPLISAKCSHE 450



 Score = 65.5 bits (158), Expect = 3e-08
 Identities = 55/228 (24%), Positives = 104/228 (45%), Gaps = 17/228 (7%)
 Frame = -2

Query: 1160 VGLVDNPILVLIYFHKALRAEFAELRRVAVEALESGTHGXXXXXXXXXXXXXXXLFYKYH 981
            + L   P+ ++ +FHKAL+ +   L   + +  E+  +                  Y+ H
Sbjct: 630  LNLEPRPVDLIFFFHKALKKDLEFLVFGSAKLAENTGY----LADFHRRFRLIRFLYQIH 685

Query: 980  SAAEDEVIFLALDELVK--NVVSAYSLEHKSIDDLFDSVFNCLDVLDK-----EGKDNYK 822
            S AEDE+ F AL+   K  N+  +Y+++HK   + F+ +   LD + K      G    K
Sbjct: 686  SDAEDEIAFPALEAKGKGQNISHSYTIDHKLEVEHFNKLSFILDEMSKLHISVSGVHFDK 745

Query: 821  TFQGLL----FCIG------TIQTTIYQHMLKEEEQVFPLLMQQFSSSEQASLVWQFMCS 672
              Q +L     C+       ++Q  +  H+  EE +++PL  + FS+ EQ  ++   +  
Sbjct: 746  MDQRMLKYHQLCMKLHDMCQSLQKILCDHVNHEEIELWPLFRECFSNKEQEKIIGSILGR 805

Query: 671  VPVTLLEDFLPWMISVLSSDEQIEVRHSLKEIVPKEKLLQEVVISWID 528
            +   +L++ +PW+++ L+  EQ     SL     K  + +E +  W D
Sbjct: 806  MRAEILQEIIPWLMASLTPKEQ-HAMMSLWRKATKNTMFEEWLGEWWD 852


>emb|CBI15293.3| unnamed protein product [Vitis vinifera]
          Length = 601

 Score =  392 bits (1007), Expect = e-106
 Identities = 223/415 (53%), Positives = 265/415 (63%), Gaps = 27/415 (6%)
 Frame = -2

Query: 1166 NGVGLVDNPILVLIYFHKALRAEFAELRRVAVEALESGTHGXXXXXXXXXXXXXXXLF-- 993
            +G  L D PIL+ ++FHKALRAE AELRR+A  A ESG  G                F  
Sbjct: 38   SGAQLRDAPILLFVFFHKALRAELAELRRLA--AAESGCRGGSSNGNLVVEIRRRFEFLK 95

Query: 992  --YKYHSAAEDEVIFLALDELVKNVVSAYSLEHKSIDDLFDSVFNCLDVLDKEGKDNYKT 819
              YKYHSAAEDEVIFLALD  +KNV   YSLEHKSIDDLF S+F+CLDVL +   +  K 
Sbjct: 96   LFYKYHSAAEDEVIFLALDVHIKNVAHTYSLEHKSIDDLFSSIFHCLDVLMEGDANTAKP 155

Query: 818  FQGLLFCIGTIQTTIYQHMLKEEEQVFPLLMQQFSSSEQASLVWQFMCSVPVTLLEDFLP 639
            FQ L+  I TIQTTI  HMLKEEEQVFPLLM+QFS  EQASLVWQFMCSVPV LLEDFLP
Sbjct: 156  FQELVLLISTIQTTICHHMLKEEEQVFPLLMKQFSPQEQASLVWQFMCSVPVLLLEDFLP 215

Query: 638  WMISVLSSDEQIEVRHSLKEIVPKEKLLQEVVISWIDNKVPVFGGCTSIREQA-----AQ 474
            WM S LS +EQ+ V + +KE+VP+EKLL+EVVISW+ N    FG  T I E+A     A 
Sbjct: 216  WMTSFLSPEEQVNVVNCIKEVVPEEKLLEEVVISWLGNNNQPFGSPTRIGEEAQSVGPAN 275

Query: 473  FHTGLGTYHETS------------------RHRSFDGIRPWHDAIRKDLQEVLVELYQIK 348
                L  Y   S                       DG+  WH AIRKDL+ +L ELYQI+
Sbjct: 276  LKDALKVYSSKSFSTEIRQWKEVFGLKTNVGQNPVDGLHLWHGAIRKDLKGILEELYQIR 335

Query: 347  ISNNFSDLPAVVVQLNFIADTLIFYSKALSNIIYPLWNELAKDFYSARYAQYLDERKIEG 168
             S++FS L +V+VQL F+AD LIFYS AL  I YPL + L+    S  Y ++ DE +IEG
Sbjct: 336  SSDSFSTLASVIVQLKFLADVLIFYSNALDKIFYPLLDLLSDGCLSPSYKRFPDESQIEG 395

Query: 167  LQRLLYYKSEKTIPLRNYVEKLYEELGSFASWINENLNLIEAEVFPLIRMNCSHD 3
            LQRLL+Y ++  IPL  +VEKL  EL SF   I ++L   E EVFPLI   CSH+
Sbjct: 396  LQRLLHYNAKNGIPLSKFVEKLCWELESFVMGIGDHLVFQEMEVFPLISAKCSHE 450


>ref|XP_002511501.1| zinc finger protein, putative [Ricinus communis]
            gi|223550616|gb|EEF52103.1| zinc finger protein, putative
            [Ricinus communis]
          Length = 1268

 Score =  325 bits (833), Expect = 2e-86
 Identities = 185/428 (43%), Positives = 256/428 (59%), Gaps = 23/428 (5%)
 Frame = -2

Query: 1217 DEVSSCGVVVQVAELPKNGVGLVDNPILVLIYFHKALRAEFAELRRVAVEALESGTHGXX 1038
            +E  S   +  V   P + V L D PIL+L+YFHKA+R E +EL R+AV A ES  +G  
Sbjct: 15   EEALSSSSMPAVESEPLSHVSLTDAPILLLVYFHKAMREELSELYRLAVLASESLPNGRQ 74

Query: 1037 XXXXXXXXXXXXXLFYKYHSAAEDEVIFLALDELVKNVVSAYSLEHKSIDDLFDSVFNCL 858
                            KYHSA EDEVIFL LD  +KN+V  YSLEH SIDD+FDS+F+CL
Sbjct: 75   LIVELRRRFDFFKHVQKYHSAFEDEVIFLELDAHIKNIVYTYSLEHNSIDDIFDSIFHCL 134

Query: 857  DVLDKEGKDNYKTFQGLLFCIGTIQTTIYQHMLKEEEQVFPLLMQQFSSSEQASLVWQFM 678
              L+ E KD  KTFQ LL CIGT+ ++I +HMLKEEEQVFPLL+Q FS  EQA LVWQF 
Sbjct: 135  STLE-ENKDGAKTFQELLSCIGTMDSSICKHMLKEEEQVFPLLIQHFSPKEQALLVWQFF 193

Query: 677  CSVPVTLLEDFLPWMISVLSSDEQIEVRHSLKEIVPKEKLLQEVVISWID-NKVPVFGGC 501
            CS+PV LL + LPW+ S L+ ++++ V   ++ +VP+EK LQEVV+SW+  N     G  
Sbjct: 194  CSIPVILLVELLPWLTSFLTPEKRLNVTRCIEGVVPQEKSLQEVVVSWLHMNGQSSLGVF 253

Query: 500  TSIREQAA----------QFHTGLGTYHE------------TSRHRSFDGIRPWHDAIRK 387
            + IR++A+          +F+    +  E             +R+   D ++ WH AI+ 
Sbjct: 254  SKIRKEASDGPECLKSMPRFYFAENSLREKRQWKKSYCVQTNARNNVIDCLKLWHRAIQT 313

Query: 386  DLQEVLVELYQIKISNNFSDLPAVVVQLNFIADTLIFYSKALSNIIYPLWNELAKDFYSA 207
            DL+E+L E Y  + S +FSD+ + +V+L F+AD +IFYS AL    YP+ NELA    S+
Sbjct: 314  DLKEILEEAYLTRNSRSFSDIDSTIVRLKFLADVIIFYSNALKKFFYPVLNELANKTCSS 373

Query: 206  RYAQYLDERKIEGLQRLLYYKSEKTIPLRNYVEKLYEELGSFASWINENLNLIEAEVFPL 27
               Q+  E ++E + +LL  K+E   P   +VEKL +EL   A  +++  +  E EV PL
Sbjct: 374  E--QFSIESRVESIHQLLQSKAENGFPFCKFVEKLCQELEFLAMDVSKKFSFQETEVLPL 431

Query: 26   IRMNCSHD 3
            I    S+D
Sbjct: 432  ISKKFSND 439



 Score = 63.2 bits (152), Expect = 1e-07
 Identities = 53/222 (23%), Positives = 95/222 (42%), Gaps = 17/222 (7%)
 Frame = -2

Query: 1142 PILVLIYFHKALRAEFAELRRVAVEALESGTHGXXXXXXXXXXXXXXXLFYKYHSAAEDE 963
            P+  + +FHKAL+ +   L   + +  E+                   L Y++HS  EDE
Sbjct: 623  PMDFIFFFHKALKKDLEYLVSGSAQLAEN----IRFLVEFSQHFHLLWLRYQFHSETEDE 678

Query: 962  VIFLALDEL--VKNVVSAYSLEHKSIDDLFDSVFNCLDVLDKEGKDNYKTFQGLL----- 804
            + F AL+    V+N+  +Y+++HK    LF+ +   L+ + K          G+L     
Sbjct: 679  IAFPALEAKGNVQNISYSYTIDHKLEVKLFNEISLILEKMSKLHVSLSTVDSGMLDQTVA 738

Query: 803  ----------FCIGTIQTTIYQHMLKEEEQVFPLLMQQFSSSEQASLVWQFMCSVPVTLL 654
                          ++   +  H+  EE +++PL  + FS  EQ  ++   +  V    L
Sbjct: 739  KYNQQCKKLHLTCKSMHKLLSDHIHHEEIELWPLFRECFSIEEQEKIIGLMIGKVGAKFL 798

Query: 653  EDFLPWMISVLSSDEQIEVRHSLKEIVPKEKLLQEVVISWID 528
            +D +PW+   L+ +EQ  V  SL   V K     E +  W++
Sbjct: 799  QDMIPWLTGSLTPEEQ-HVLMSLWRKVTKNTKFDEWLGEWLE 839


>ref|XP_004138295.1| PREDICTED: uncharacterized protein LOC101212459 [Cucumis sativus]
            gi|449477600|ref|XP_004155068.1| PREDICTED:
            uncharacterized protein LOC101229801 [Cucumis sativus]
          Length = 1252

 Score =  324 bits (830), Expect = 4e-86
 Identities = 177/389 (45%), Positives = 249/389 (64%), Gaps = 4/389 (1%)
 Frame = -2

Query: 1160 VGLVDNPILVLIYFHKALRAEFAELRRVAVEALESGTHGXXXXXXXXXXXXXXXLFYKYH 981
            V L + PIL+LI FH+ALR E A+LRRV + A ESG +G               L YKYH
Sbjct: 38   VSLTEAPILLLIKFHQALRLEVADLRRVTLAAAESGGYGGEFVSGLIRRVEFLKLAYKYH 97

Query: 980  SAAEDEVIFLALDELVKNVVSAYSLEHKSIDDLFDSVFNCLDVLDKEGKDNYKTFQGLLF 801
             AAEDEV+F ALD   KNV+S YSLEH+S+D LF S+    + ++ E KD  K FQ L+F
Sbjct: 98   CAAEDEVVFPALDLHTKNVISTYSLEHESLDGLFTSISKLCEDINGENKDISKPFQELIF 157

Query: 800  CIGTIQTTIYQHMLKEEEQVFPLLMQQFSSSEQASLVWQFMCSVPVTLLEDFLPWMISVL 621
            C+GTIQTTI QHM+KEE+QVFPLLM++FS+ EQASLVWQF+CSVP+ LLE+ LPWM+S L
Sbjct: 158  CLGTIQTTICQHMIKEEQQVFPLLMKEFSAREQASLVWQFICSVPMILLEELLPWMMSFL 217

Query: 620  SSDEQIEVRHSLKEIVPKEKLLQEVVISWIDNKVPVFGG--CTSIREQAAQFHTGLGTYH 447
             +D+Q EV + L+++VP EKLLQEV++SW+ +    +       I+ Q++Q         
Sbjct: 218  PADQQSEVVNCLRDVVPNEKLLQEVIMSWLGSTEKPWRDVEVEDIKLQSSQ--------- 268

Query: 446  ETSRHRSFDGIRPWHDAIRKDLQEVLVELYQIK--ISNNFSDLPAVVVQLNFIADTLIFY 273
              +     D +  WH AI KDL+EVL  L+Q+K   S   S+L  ++VQ+ F+AD ++FY
Sbjct: 269  -ENGQSPVDSLHIWHGAIMKDLKEVLKCLFQVKSCTSTALSNLDTLLVQIKFLADVILFY 327

Query: 272  SKALSNIIYPLWNELAKDFYSARYAQYLDERKIEGLQRLLYYKSEKTIPLRNYVEKLYEE 93
             KA      P++N+ +    +     +L +  IEGLQ+LL + ++ TIPL  ++EKL  +
Sbjct: 328  RKASEKFFCPVFNQRSDVCLTTSDQSFLSDGHIEGLQQLLQHGAQDTIPLSIFLEKLCWD 387

Query: 92   LGSFASWINENLNLIEAEVFPLIRMNCSH 6
            + SF   +++     E +V P+IR +CSH
Sbjct: 388  MESFVIRVSKQFTFQETKVLPVIRKSCSH 416



 Score = 62.4 bits (150), Expect = 3e-07
 Identities = 48/217 (22%), Positives = 96/217 (44%), Gaps = 14/217 (6%)
 Frame = -2

Query: 1142 PILVLIYFHKALRAEFAELRRVAVEALESGTHGXXXXXXXXXXXXXXXLFYKYHSAAEDE 963
            PI ++ +FHKAL+ E       + + +E                      Y+ H+ AED+
Sbjct: 604  PIDLIFFFHKALKKELDYFVLGSAKLVEH----VGILTEFRRRFQLVKFLYQIHTDAEDQ 659

Query: 962  VIFLALDELVK--NVVSAYSLEHKSIDDLFDSVFNCLDVLDKEGKDNY------KTFQGL 807
            + F AL++  K  N+  +Y+++HK     F  +   L  + +    N+      K F   
Sbjct: 660  IAFPALEKKGKFQNISYSYTIDHKLEVHQFSKISFVLSEMSELHSSNFYVNADRKIFSHR 719

Query: 806  LFCIG------TIQTTIYQHMLKEEEQVFPLLMQQFSSSEQASLVWQFMCSVPVTLLEDF 645
              C+       ++  ++  H+ +EE +++PL  + F+  EQ +L+          +L+D 
Sbjct: 720  QLCLELHDMCKSLHKSLSDHVDREEIELWPLFREFFTIDEQETLIGAIFGRTKAEILQDM 779

Query: 644  LPWMISVLSSDEQIEVRHSLKEIVPKEKLLQEVVISW 534
            +PW +S L+  +Q ++  S+   V +  +  E +  W
Sbjct: 780  IPWQMSYLTPSDQHDMM-SMFHKVTRNTMFNEWLREW 815


>ref|XP_003550768.1| PREDICTED: uncharacterized protein LOC100802706 [Glycine max]
          Length = 1262

 Score =  316 bits (810), Expect = 7e-84
 Identities = 186/419 (44%), Positives = 249/419 (59%), Gaps = 30/419 (7%)
 Frame = -2

Query: 1178 ELPKNGVGLVDNPILVLIYFHKALRAEFAELRRVAVEA--LESGTHGXXXXXXXXXXXXX 1005
            + P   V LVD PIL+ + FHKA R+E   LRR+A  A  LE                  
Sbjct: 20   DTPLLRVPLVDAPILLFVCFHKAFRSELDHLRRLAETASSLEDEPRRCRQIVLQLQRRFQ 79

Query: 1004 XXLF-YKYHSAAEDEVIFLALDELVKNVVSAYSLEHKSIDDLFDSVFNCLDVLDKEGKDN 828
                 +KYH AAEDEVIFLALD  VKNV+  YSLEH+S + LF SVF+ LD L    ++ 
Sbjct: 80   FLKLAHKYHCAAEDEVIFLALDTHVKNVICTYSLEHRSTNGLFGSVFHFLDELMVPKENI 139

Query: 827  YKTFQGLLFCIGTIQTTIYQHMLKEEEQVFPLLMQQFSSSEQASLVWQFMCSVPVTLLED 648
             K FQ L++CIG +QT+IYQHMLKEEEQVFPLL+Q+ S+ EQASLVWQF+CSVP+ LLE+
Sbjct: 140  SKLFQELVYCIGILQTSIYQHMLKEEEQVFPLLIQKLSNKEQASLVWQFICSVPIMLLEE 199

Query: 647  FLPWMISVLSSDEQIEVRHSLKEIVPKEKLLQEVVISWIDNKVPVFGGCTSIREQAAQFH 468
             LPWM+S LS+++Q EV   L EI P EK +QEV++SW+ +       CT    Q+ +F 
Sbjct: 200  VLPWMVSFLSANKQSEVTQCLNEIAPMEKAMQEVLVSWLRSSKQT---CTETCFQSGEFQ 256

Query: 467  TGLGTYH-ETSRHRSF--------------------DG------IRPWHDAIRKDLQEVL 369
               G  H E S   S+                    DG      +  WH+AI+KDL+++L
Sbjct: 257  GVDGFLHIERSLELSYCNRNSEEISSPMKVNGKEIEDGANQVNVLHLWHNAIKKDLKDIL 316

Query: 368  VELYQIKISNNFSDLPAVVVQLNFIADTLIFYSKALSNIIYPLWNELAKDFYSARYAQYL 189
             EL+ ++ S+ F +L ++++QL F AD LIFYS A     +P+ N+ A  + S    Q+L
Sbjct: 317  EELHLLRKSSCFQNLDSILIQLKFFADVLIFYSDAQKKFFHPVLNKHAYGWLSKSIEQFL 376

Query: 188  DERKIEGLQRLLYYKSEKTIPLRNYVEKLYEELGSFASWINENLNLIEAEVFPLIRMNC 12
             E  IE +Q+LL+Y SE  I L  ++EKL + L SF S +N+     E EVFP+ R NC
Sbjct: 377  GESNIEDIQQLLFYNSESGILLSKFIEKLCQTLESFVSGVNKQFAFQENEVFPIFRKNC 435



 Score = 68.2 bits (165), Expect = 5e-09
 Identities = 62/276 (22%), Positives = 125/276 (45%), Gaps = 27/276 (9%)
 Frame = -2

Query: 1274 ETP-----SLWLFFDIEIAKI-----MSGDEVSSCGVVVQVAELPKNGVGLVDNPILVLI 1125
            ETP     +L +FF   +AK+     +  +E SS    +   + PK        PI ++ 
Sbjct: 569  ETPYSTGINLHIFFPSTVAKLHQHPTLHAEERSS----ISFLDDPK--------PIDLIF 616

Query: 1124 YFHKALRAEFAELRRVAVEALESGTHGXXXXXXXXXXXXXXXLFYKYHSAAEDEVIFLAL 945
            +FHKA++ +   L   + +  ++                     ++ HS AEDE++F A+
Sbjct: 617  FFHKAIKKDLEYLVLGSTQLEKNDK----LLMDFHKRFHLIYFLHQIHSDAEDEIVFPAM 672

Query: 944  DEL--VKNVVSAYSLEHKSIDDLFDSVFNCLD----------VLDKEGKDN-----YKTF 816
            +    +KN+  AY+ +HK   D F+ + + LD           +D   K+      +   
Sbjct: 673  EARGKLKNISHAYTFDHKHEVDHFNKISHILDKMSGLHLSVSTIDPNVKEKGILRYHHLC 732

Query: 815  QGLLFCIGTIQTTIYQHMLKEEEQVFPLLMQQFSSSEQASLVWQFMCSVPVTLLEDFLPW 636
            + L     ++  ++  H+ +EE +++P++ + FS+ EQ  ++   +  +   +L+D +PW
Sbjct: 733  RKLQEMCKSMHKSLSDHINREEIEIWPIIRKFFSNHEQGRIIGCMLGRIRAEILQDMIPW 792

Query: 635  MISVLSSDEQIEVRHSLKEIVPKEKLLQEVVISWID 528
            +++ L+ +EQ  V   L  +  K  +  E +  W D
Sbjct: 793  LMASLTQEEQ-HVLMFLWSMATKNTMFDEWLGEWWD 827