BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cnidium21_contig00023715
         (502 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O48881.1|TPS1_BRANA RecName: Full=Thiamine biosynthetic bifun...    69   5e-10
ref|NP_173707.2| thiamine-phosphate pyrophosphorylase [Arabidops...    64   1e-08
gb|AAM91567.1| phosphomethylpyrimidine kinase [Arabidopsis thali...    64   1e-08
ref|XP_002265950.2| PREDICTED: putative hydroxymethylpyrimidine/...    63   3e-08
ref|XP_002890546.1| hypothetical protein ARALYDRAFT_472545 [Arab...    63   3e-08

>sp|O48881.1|TPS1_BRANA RecName: Full=Thiamine biosynthetic bifunctional enzyme BTH1,
           chloroplastic; Includes: RecName:
           Full=Thiamine-phosphate synthase; Short=TP synthase;
           Short=TPS; AltName: Full=Thiamine-phosphate
           pyrophosphorylase; Short=TMP pyrophosphorylase;
           Short=TMP-PPase; Includes: RecName:
           Full=Hydroxymethylpyrimidine kinase; Short=HMP kinase;
           Flags: Precursor gi|2746079|gb|AAC31298.1| BTH1
           [Brassica napus]
          Length = 523

 Score = 68.6 bits (166), Expect = 5e-10
 Identities = 38/88 (43%), Positives = 58/88 (65%), Gaps = 8/88 (9%)
 Frame = +3

Query: 225 TPDRNEIMLVLLSVYPVSTVITSYHVAPVHKAMQG--------IGTLRDQLLFMADIVTP 380
           +P+  E++L  LS YPV  ++    V PV  +  G        +   R++LL +ADI+TP
Sbjct: 112 SPEIVEVLLQNLSEYPVRALV----VDPVMVSTSGHVLAGSSILSIFRERLLPLADIITP 167

Query: 381 NLKEATTLLNGIKLQTVSDMRSAAKSIH 464
           N+KEA+ LL G+++QTV++MRSAAKS+H
Sbjct: 168 NVKEASALLGGVRIQTVAEMRSAAKSLH 195


>ref|NP_173707.2| thiamine-phosphate pyrophosphorylase [Arabidopsis thaliana]
           gi|75222969|sp|Q5M731.1|TPS1L_ARATH RecName:
           Full=Thiamine biosynthetic bifunctional enzyme TH1,
           chloroplastic; Includes: RecName:
           Full=Thiamine-phosphate synthase; Short=TP synthase;
           Short=TPS; AltName: Full=Thiamine-phosphate
           pyrophosphorylase; Short=TMP pyrophosphorylase;
           Short=TMP-PPase; Includes: RecName:
           Full=Hydroxymethylpyrimidine kinase; Short=HMP kinase;
           Flags: Precursor gi|56550709|gb|AAV97808.1| At1g22940
           [Arabidopsis thaliana] gi|332192192|gb|AEE30313.1|
           thiamine-phosphate pyrophosphorylase [Arabidopsis
           thaliana]
          Length = 522

 Score = 63.9 bits (154), Expect = 1e-08
 Identities = 41/117 (35%), Positives = 64/117 (54%), Gaps = 30/117 (25%)
 Frame = +3

Query: 240 EIMLVLLSVYPVSTVITSYHVAPVHKAMQG--------IGTLRDQLLFMADIVTPNLKEA 395
           E++L  LS +PV  ++    V PV  +  G        +   R++LL +ADI+TPN+KEA
Sbjct: 115 EVLLQNLSDFPVRALV----VDPVMVSTSGHVLAGSSILSIFRERLLPIADIITPNVKEA 170

Query: 396 TTLLNGIKLQTVSDMRSAAKSIH----------------------IWVQGMEFYELR 500
           + LL+G +++TV++MRSAAKS+H                      ++  G EF+ELR
Sbjct: 171 SALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLPDSSDSVDVYFDGKEFHELR 227


>gb|AAM91567.1| phosphomethylpyrimidine kinase [Arabidopsis thaliana]
          Length = 522

 Score = 63.9 bits (154), Expect = 1e-08
 Identities = 41/117 (35%), Positives = 64/117 (54%), Gaps = 30/117 (25%)
 Frame = +3

Query: 240 EIMLVLLSVYPVSTVITSYHVAPVHKAMQG--------IGTLRDQLLFMADIVTPNLKEA 395
           E++L  LS +PV  ++    V PV  +  G        +   R++LL +ADI+TPN+KEA
Sbjct: 115 EVLLQNLSDFPVRALV----VDPVMVSTSGHVLAGSSILSIFRERLLPIADIITPNVKEA 170

Query: 396 TTLLNGIKLQTVSDMRSAAKSIH----------------------IWVQGMEFYELR 500
           + LL+G +++TV++MRSAAKS+H                      ++  G EF+ELR
Sbjct: 171 SALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLPDSSDSVDVYFDGKEFHELR 227


>ref|XP_002265950.2| PREDICTED: putative hydroxymethylpyrimidine/phosphomethylpyrimidine
           kinase 2-like [Vitis vinifera]
          Length = 681

 Score = 62.8 bits (151), Expect = 3e-08
 Identities = 52/143 (36%), Positives = 68/143 (47%), Gaps = 33/143 (23%)
 Frame = +3

Query: 171 EEKMCFLSSSHKKVVSHQTPDRNEIMLVL---LSVYPVSTVITSYHVAPVHKAMQG---- 329
           E+    LS  H  VV         I+ VL   L  +PV  ++    V PV  +  G    
Sbjct: 246 EQLKSVLSDMHVDVVKTGMLPTIGIVKVLHHSLKEFPVQALV----VDPVMVSTSGDVLA 301

Query: 330 ----IGTLRDQLLFMADIVTPNLKEATTLLNGIKLQTVSDMRSAAKSIH----------- 464
               +   R++LL MADIVTPNLKEA+ LL G++L+TVSDM +AAK IH           
Sbjct: 302 GPSILAAFREELLPMADIVTPNLKEASALLGGLQLETVSDMCTAAKLIHDMGPRNVLVKG 361

Query: 465 -----------IWVQGMEFYELR 500
                      I+  G +FYELR
Sbjct: 362 GDLPSSLDAVDIFFDGDDFYELR 384


>ref|XP_002890546.1| hypothetical protein ARALYDRAFT_472545 [Arabidopsis lyrata subsp.
           lyrata] gi|297336388|gb|EFH66805.1| hypothetical protein
           ARALYDRAFT_472545 [Arabidopsis lyrata subsp. lyrata]
          Length = 522

 Score = 62.8 bits (151), Expect = 3e-08
 Identities = 41/117 (35%), Positives = 62/117 (52%), Gaps = 30/117 (25%)
 Frame = +3

Query: 240 EIMLVLLSVYPVSTVITSYHVAPVHKAMQG--------IGTLRDQLLFMADIVTPNLKEA 395
           E++L  LS +PV  ++    V PV  +  G        +   R++LL +ADI+TPN+KEA
Sbjct: 115 EVLLQNLSDFPVRALV----VDPVMVSTSGHVLAGSSILSIFRERLLPIADIITPNVKEA 170

Query: 396 TTLLNGIKLQTVSDMRSAAKSIH----------------------IWVQGMEFYELR 500
           + LL G +++TV+DMRSAAK +H                      ++  G EF+ELR
Sbjct: 171 SALLGGFRIETVADMRSAAKLLHEMGPRFVLVKGGDLPDSSNSVDVYFDGKEFHELR 227