BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cnidium21_contig00021976
         (778 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAN70930.1| hypothetical protein VITISV_000387 [Vitis vinifera]   306   4e-81
ref|XP_002280644.1| PREDICTED: pentatricopeptide repeat-containi...   303   3e-80
ref|XP_003530188.1| PREDICTED: pentatricopeptide repeat-containi...   292   6e-77
ref|NP_188050.1| pentatricopeptide repeat-containing protein [Ar...   258   1e-66
dbj|BAB01039.1| unnamed protein product [Arabidopsis thaliana]        258   1e-66

>emb|CAN70930.1| hypothetical protein VITISV_000387 [Vitis vinifera]
          Length = 773

 Score =  306 bits (783), Expect = 4e-81
 Identities = 156/262 (59%), Positives = 200/262 (76%), Gaps = 5/262 (1%)
 Frame = -2

Query: 777 LDEALHLIESNPTS--LNEPNIDAYSSFLHSCISKKSLHHGQRLYLQLLLSKNKTHQNLL 604
           LDEAL LIES P+      P+++A + FLHSCIS+K+L HGQRLYLQLLL +++ + NLL
Sbjct: 46  LDEALRLIESWPSKSPATAPDVEACALFLHSCISRKALEHGQRLYLQLLLYRDRCNHNLL 105

Query: 603 QNPIIKSKLITLYTVCGEIDEARRVFDDGKSS---DEAVWVAMAIGYSRKGLFRESLELY 433
            NP +K KLITL++VCG +DEARRVF+DG       E+VWVAM IGYSR G  +E+L LY
Sbjct: 106 NNPTLKGKLITLFSVCGRVDEARRVFEDGGEDVDLPESVWVAMGIGYSRNGYPKEALLLY 165

Query: 432 CEMLGQCVWPSNFGFSTALKACSELGEVGIGRMVHAQIVKAGDEVDQVVFNSLLRLYNHC 253
            EM+ Q     NF FS ALKACS+LG++  GR VHAQ++KA ++ DQVV N+LLRLY+  
Sbjct: 166 YEMVCQFGQLGNFAFSMALKACSDLGDLRTGRAVHAQVLKATEDPDQVVNNALLRLYSED 225

Query: 252 GCFDDVVKLFDEMPQRNVVTWNSLIDGLVKRGQLFEAFKLFRKMQVEGTGFSWVTLTTVL 73
           GCF++ +++FD MP RN+V+WNSLI GLVK+  +FEA + FR MQ +G GFSWVTLTT+L
Sbjct: 226 GCFEEALRMFDGMPHRNLVSWNSLIAGLVKKEGVFEAIEAFRIMQGKGMGFSWVTLTTIL 285

Query: 72  SACAKVTAIYSGKEVHAQIVKS 7
             CA+VTA+ SGKE+HA IVKS
Sbjct: 286 PVCARVTALGSGKEIHAVIVKS 307



 Score =  100 bits (250), Expect = 3e-19
 Identities = 62/218 (28%), Positives = 111/218 (50%)
 Frame = -2

Query: 714 AYSSFLHSCISKKSLHHGQRLYLQLLLSKNKTHQNLLQNPIIKSKLITLYTVCGEIDEAR 535
           A+S  L +C     L  G+ ++ Q+L +     Q      ++ + L+ LY+  G  +EA 
Sbjct: 179 AFSMALKACSDLGDLRTGRAVHAQVLKATEDPDQ------VVNNALLRLYSEDGCFEEAL 232

Query: 534 RVFDDGKSSDEAVWVAMAIGYSRKGLFRESLELYCEMLGQCVWPSNFGFSTALKACSELG 355
           R+FD     +   W ++  G  +K    E++E +  M G+ +  S    +T L  C+ + 
Sbjct: 233 RMFDGMPHRNLVSWNSLIAGLVKKEGVFEAIEAFRIMQGKGMGFSWVTLTTILPVCARVT 292

Query: 354 EVGIGRMVHAQIVKAGDEVDQVVFNSLLRLYNHCGCFDDVVKLFDEMPQRNVVTWNSLID 175
            +G G+ +HA IVK+  + D  V NSL+ +Y  CG  D   ++F+ M  +++ +WN+LI 
Sbjct: 293 ALGSGKEIHAVIVKSTAKPDAPVLNSLVDMYAKCGAMDYCRRVFNGMQGKDLTSWNTLIT 352

Query: 174 GLVKRGQLFEAFKLFRKMQVEGTGFSWVTLTTVLSACA 61
           G    G++ EA + F++M   G     +T   +LS C+
Sbjct: 353 GYAINGRMTEAMESFQEMICSGFSPDGITFIALLSGCS 390


>ref|XP_002280644.1| PREDICTED: pentatricopeptide repeat-containing protein At3g14330
           [Vitis vinifera] gi|296088358|emb|CBI36803.3| unnamed
           protein product [Vitis vinifera]
          Length = 652

 Score =  303 bits (776), Expect = 3e-80
 Identities = 156/262 (59%), Positives = 198/262 (75%), Gaps = 5/262 (1%)
 Frame = -2

Query: 777 LDEALHLIESNPTS--LNEPNIDAYSSFLHSCISKKSLHHGQRLYLQLLLSKNKTHQNLL 604
           LDEAL LIE  P+      P+++A + FLHSCIS+K+L HGQRLYLQLLL +++ + NLL
Sbjct: 47  LDEALRLIEYWPSKSPATAPDVEACALFLHSCISRKALEHGQRLYLQLLLYRDRCNHNLL 106

Query: 603 QNPIIKSKLITLYTVCGEIDEARRVFDDGKSS---DEAVWVAMAIGYSRKGLFRESLELY 433
            NP +K KLITL++VC  +DEARRVF+DG       E+VWVAM IGYSR G  +E+L LY
Sbjct: 107 NNPTLKGKLITLFSVCRRVDEARRVFEDGGEDVDLPESVWVAMGIGYSRNGYPKEALLLY 166

Query: 432 CEMLGQCVWPSNFGFSTALKACSELGEVGIGRMVHAQIVKAGDEVDQVVFNSLLRLYNHC 253
            EM+ Q     NF FS ALKACS+LG++  GR VHAQ++KA ++ DQVV N+LLRLY+  
Sbjct: 167 YEMVCQFGQLGNFAFSMALKACSDLGDLQTGRAVHAQVLKATEDPDQVVNNALLRLYSED 226

Query: 252 GCFDDVVKLFDEMPQRNVVTWNSLIDGLVKRGQLFEAFKLFRKMQVEGTGFSWVTLTTVL 73
           GCFD+ +++FD MP RNVV+WNSLI GLVK+  +FEA + FR MQ +G GFSWVTLTT+L
Sbjct: 227 GCFDEALRVFDGMPHRNVVSWNSLIAGLVKKDGVFEAIEAFRIMQGKGMGFSWVTLTTIL 286

Query: 72  SACAKVTAIYSGKEVHAQIVKS 7
             CA+VTA+ SGKE+HA IVKS
Sbjct: 287 PVCARVTALGSGKEIHAVIVKS 308



 Score =  104 bits (259), Expect = 2e-20
 Identities = 64/218 (29%), Positives = 111/218 (50%)
 Frame = -2

Query: 714 AYSSFLHSCISKKSLHHGQRLYLQLLLSKNKTHQNLLQNPIIKSKLITLYTVCGEIDEAR 535
           A+S  L +C     L  G+ ++ Q+L +     Q      ++ + L+ LY+  G  DEA 
Sbjct: 180 AFSMALKACSDLGDLQTGRAVHAQVLKATEDPDQ------VVNNALLRLYSEDGCFDEAL 233

Query: 534 RVFDDGKSSDEAVWVAMAIGYSRKGLFRESLELYCEMLGQCVWPSNFGFSTALKACSELG 355
           RVFD     +   W ++  G  +K    E++E +  M G+ +  S    +T L  C+ + 
Sbjct: 234 RVFDGMPHRNVVSWNSLIAGLVKKDGVFEAIEAFRIMQGKGMGFSWVTLTTILPVCARVT 293

Query: 354 EVGIGRMVHAQIVKAGDEVDQVVFNSLLRLYNHCGCFDDVVKLFDEMPQRNVVTWNSLID 175
            +G G+ +HA IVK+  + D  V NSL+ +Y  CG  D   ++F+ M  +++ +WN+LI 
Sbjct: 294 ALGSGKEIHAVIVKSTAKPDAPVLNSLVDMYAKCGAMDYCRRVFNGMQGKDLTSWNTLIT 353

Query: 174 GLVKRGQLFEAFKLFRKMQVEGTGFSWVTLTTVLSACA 61
           G    G++ EA + F++M   G     +T   +LS C+
Sbjct: 354 GYAINGRMTEAMESFQEMICSGFSPDGITFIALLSGCS 391


>ref|XP_003530188.1| PREDICTED: pentatricopeptide repeat-containing protein
           At3g14330-like [Glycine max]
          Length = 650

 Score =  292 bits (747), Expect = 6e-77
 Identities = 153/262 (58%), Positives = 196/262 (74%), Gaps = 5/262 (1%)
 Frame = -2

Query: 777 LDEALHLIESN-PTSLNEPNID-AYSSFLHSCISKKSLHHGQRLYLQLLLSKNKTHQNLL 604
           LD+AL LIES+ PT + E   + + S FLH+CIS++SL HG++L+L LL S+N+    +L
Sbjct: 49  LDKALRLIESSKPTPIEEEEEEESISLFLHACISRRSLEHGRKLHLHLLRSQNR----VL 104

Query: 603 QNPIIKSKLITLYTVCGEIDEARRVF--DDGKSSDEAVWVAMAIGYSRKGLFRESLELYC 430
           +NP +K+KLITLY+VCG ++EARRVF  DD K  +E VWVAMAIGYSR G   E+L LY 
Sbjct: 105 ENPTLKTKLITLYSVCGRVNEARRVFQIDDEKPPEEPVWVAMAIGYSRNGFSHEALLLYR 164

Query: 429 EMLGQCVWPSNFGFSTALKACSELGEVGIGRMVHAQIVKAG-DEVDQVVFNSLLRLYNHC 253
           +ML  CV P NF FS ALKACS+L    +GR +HAQIVK    E DQVV N+LL LY   
Sbjct: 165 DMLSCCVKPGNFAFSMALKACSDLDNALVGRAIHAQIVKHDVGEADQVVNNALLGLYVEI 224

Query: 252 GCFDDVVKLFDEMPQRNVVTWNSLIDGLVKRGQLFEAFKLFRKMQVEGTGFSWVTLTTVL 73
           GCFD+V+K+F+EMPQRNVV+WN+LI G   +G++FE    FR MQ EG GFSW+TLTT+L
Sbjct: 225 GCFDEVLKVFEEMPQRNVVSWNTLIAGFAGQGRVFETLSAFRVMQREGMGFSWITLTTML 284

Query: 72  SACAKVTAIYSGKEVHAQIVKS 7
             CA+VTA++SGKE+H QI+KS
Sbjct: 285 PVCAQVTALHSGKEIHGQILKS 306



 Score = 99.8 bits (247), Expect = 6e-19
 Identities = 64/257 (24%), Positives = 122/257 (47%)
 Frame = -2

Query: 771 EALHLIESNPTSLNEPNIDAYSSFLHSCISKKSLHHGQRLYLQLLLSKNKTHQNLLQNPI 592
           EAL L     +   +P   A+S  L +C    +   G+ ++ Q++      H     + +
Sbjct: 158 EALLLYRDMLSCCVKPGNFAFSMALKACSDLDNALVGRAIHAQIV-----KHDVGEADQV 212

Query: 591 IKSKLITLYTVCGEIDEARRVFDDGKSSDEAVWVAMAIGYSRKGLFRESLELYCEMLGQC 412
           + + L+ LY   G  DE  +VF++    +   W  +  G++ +G   E+L  +  M  + 
Sbjct: 213 VNNALLGLYVEIGCFDEVLKVFEEMPQRNVVSWNTLIAGFAGQGRVFETLSAFRVMQREG 272

Query: 411 VWPSNFGFSTALKACSELGEVGIGRMVHAQIVKAGDEVDQVVFNSLLRLYNHCGCFDDVV 232
           +  S    +T L  C+++  +  G+ +H QI+K+    D  + NSL+ +Y  CG      
Sbjct: 273 MGFSWITLTTMLPVCAQVTALHSGKEIHGQILKSRKNADVPLLNSLMDMYAKCGEIGYCE 332

Query: 231 KLFDEMPQRNVVTWNSLIDGLVKRGQLFEAFKLFRKMQVEGTGFSWVTLTTVLSACAKVT 52
           K+FD M  +++ +WN+++ G    GQ+ EA  LF +M   G   + +T   +LS C+   
Sbjct: 333 KVFDRMHSKDLTSWNTMLAGFSINGQIHEALCLFDEMIRYGIEPNGITFVALLSGCSHSG 392

Query: 51  AIYSGKEVHAQIVKSCG 1
               GK + + +++  G
Sbjct: 393 LTSEGKRLFSNVMQDFG 409


>ref|NP_188050.1| pentatricopeptide repeat-containing protein [Arabidopsis thaliana]
           gi|218546762|sp|Q9LUL5.2|PP229_ARATH RecName:
           Full=Pentatricopeptide repeat-containing protein
           At3g14330 gi|332641981|gb|AEE75502.1| pentatricopeptide
           repeat-containing protein [Arabidopsis thaliana]
          Length = 710

 Score =  258 bits (659), Expect = 1e-66
 Identities = 139/263 (52%), Positives = 184/263 (69%), Gaps = 6/263 (2%)
 Frame = -2

Query: 777 LDEALHLIE---SNPTSLNEPNIDAYSSFLHSCISKKSLHHGQRLYLQLLLSKNKTHQNL 607
           LDEA+ LIE   S+P++L+ P  +AY+  LH+CIS KSLHHG ++   +L + +  H   
Sbjct: 111 LDEAVTLIENSSSSPSNLSTP--EAYTDLLHACISAKSLHHGIKICSLILNNPSLRH--- 165

Query: 606 LQNPIIKSKLITLYTVCGEIDEARRVFDDGKSSD---EAVWVAMAIGYSRKGLFRESLEL 436
             NP + SKLITL++VC  +D AR++FDD   S    E VW AMAIGYSR G  R++L +
Sbjct: 166 --NPKLLSKLITLFSVCRRLDLARKIFDDVTDSSLLTEKVWAAMAIGYSRNGSPRDALIV 223

Query: 435 YCEMLGQCVWPSNFGFSTALKACSELGEVGIGRMVHAQIVKAGDEVDQVVFNSLLRLYNH 256
           Y +ML   + P NF  S ALKAC +L ++ +GR +HAQIVK  ++VDQVV+N LL+LY  
Sbjct: 224 YVDMLCSFIEPGNFSISVALKACVDLKDLRVGRGIHAQIVKRKEKVDQVVYNVLLKLYME 283

Query: 255 CGCFDDVVKLFDEMPQRNVVTWNSLIDGLVKRGQLFEAFKLFRKMQVEGTGFSWVTLTTV 76
            G FDD  K+FD M +RNVVTWNSLI  L K+ ++ E F LFRKMQ E  GFSW TLTT+
Sbjct: 284 SGLFDDARKVFDGMSERNVVTWNSLISVLSKKVRVHEMFNLFRKMQEEMIGFSWATLTTI 343

Query: 75  LSACAKVTAIYSGKEVHAQIVKS 7
           L AC++V A+ +GKE+HAQI+KS
Sbjct: 344 LPACSRVAALLTGKEIHAQILKS 366



 Score =  104 bits (259), Expect = 2e-20
 Identities = 63/226 (27%), Positives = 111/226 (49%)
 Frame = -2

Query: 738 SLNEPNIDAYSSFLHSCISKKSLHHGQRLYLQLLLSKNKTHQNLLQNPIIKSKLITLYTV 559
           S  EP   + S  L +C+  K L  G+ ++ Q++  K K  Q      ++ + L+ LY  
Sbjct: 230 SFIEPGNFSISVALKACVDLKDLRVGRGIHAQIVKRKEKVDQ------VVYNVLLKLYME 283

Query: 558 CGEIDEARRVFDDGKSSDEAVWVAMAIGYSRKGLFRESLELYCEMLGQCVWPSNFGFSTA 379
            G  D+AR+VFD     +   W ++    S+K    E   L+ +M  + +  S    +T 
Sbjct: 284 SGLFDDARKVFDGMSERNVVTWNSLISVLSKKVRVHEMFNLFRKMQEEMIGFSWATLTTI 343

Query: 378 LKACSELGEVGIGRMVHAQIVKAGDEVDQVVFNSLLRLYNHCGCFDDVVKLFDEMPQRNV 199
           L ACS +  +  G+ +HAQI+K+ ++ D  + NSL+ +Y  CG  +   ++FD M  +++
Sbjct: 344 LPACSRVAALLTGKEIHAQILKSKEKPDVPLLNSLMDMYGKCGEVEYSRRVFDVMLTKDL 403

Query: 198 VTWNSLIDGLVKRGQLFEAFKLFRKMQVEGTGFSWVTLTTVLSACA 61
            +WN +++     G + E   LF  M   G     +T   +LS C+
Sbjct: 404 ASWNIMLNCYAINGNIEEVINLFEWMIESGVAPDGITFVALLSGCS 449



 Score = 63.5 bits (153), Expect = 5e-08
 Identities = 50/189 (26%), Positives = 85/189 (44%), Gaps = 3/189 (1%)
 Frame = -2

Query: 708 SSFLHSCISKKSLHHGQRLYLQLLLSKNKTHQNLLQNPIIKSKLITLYTVCGEIDEARRV 529
           ++ L +C    +L  G+ ++ Q+L SK K    LL +      L+ +Y  CGE++ +RRV
Sbjct: 341 TTILPACSRVAALLTGKEIHAQILKSKEKPDVPLLNS------LMDMYGKCGEVEYSRRV 394

Query: 528 FDDGKSSDEAVWVAMAIGYSRKGLFRESLELYCEMLGQCVWPSNFGFSTALKACSELGEV 349
           FD   + D A W  M   Y+  G   E + L+  M+   V P    F   L  CS+ G  
Sbjct: 395 FDVMLTKDLASWNIMLNCYAINGNIEEVINLFEWMIESGVAPDGITFVALLSGCSDTGLT 454

Query: 348 GIGRMVHAQIVKAGDEVDQVV--FNSLLRLYNHCGCFDDVVKLFDEMP-QRNVVTWNSLI 178
             G  +  + +K    V   +  +  L+ +    G   + VK+ + MP + +   W SL+
Sbjct: 455 EYGLSLFER-MKTEFRVSPALEHYACLVDILGRAGKIKEAVKVIETMPFKPSASIWGSLL 513

Query: 177 DGLVKRGQL 151
           +     G +
Sbjct: 514 NSCRLHGNV 522


>dbj|BAB01039.1| unnamed protein product [Arabidopsis thaliana]
          Length = 717

 Score =  258 bits (659), Expect = 1e-66
 Identities = 139/263 (52%), Positives = 184/263 (69%), Gaps = 6/263 (2%)
 Frame = -2

Query: 777 LDEALHLIE---SNPTSLNEPNIDAYSSFLHSCISKKSLHHGQRLYLQLLLSKNKTHQNL 607
           LDEA+ LIE   S+P++L+ P  +AY+  LH+CIS KSLHHG ++   +L + +  H   
Sbjct: 118 LDEAVTLIENSSSSPSNLSTP--EAYTDLLHACISAKSLHHGIKICSLILNNPSLRH--- 172

Query: 606 LQNPIIKSKLITLYTVCGEIDEARRVFDDGKSSD---EAVWVAMAIGYSRKGLFRESLEL 436
             NP + SKLITL++VC  +D AR++FDD   S    E VW AMAIGYSR G  R++L +
Sbjct: 173 --NPKLLSKLITLFSVCRRLDLARKIFDDVTDSSLLTEKVWAAMAIGYSRNGSPRDALIV 230

Query: 435 YCEMLGQCVWPSNFGFSTALKACSELGEVGIGRMVHAQIVKAGDEVDQVVFNSLLRLYNH 256
           Y +ML   + P NF  S ALKAC +L ++ +GR +HAQIVK  ++VDQVV+N LL+LY  
Sbjct: 231 YVDMLCSFIEPGNFSISVALKACVDLKDLRVGRGIHAQIVKRKEKVDQVVYNVLLKLYME 290

Query: 255 CGCFDDVVKLFDEMPQRNVVTWNSLIDGLVKRGQLFEAFKLFRKMQVEGTGFSWVTLTTV 76
            G FDD  K+FD M +RNVVTWNSLI  L K+ ++ E F LFRKMQ E  GFSW TLTT+
Sbjct: 291 SGLFDDARKVFDGMSERNVVTWNSLISVLSKKVRVHEMFNLFRKMQEEMIGFSWATLTTI 350

Query: 75  LSACAKVTAIYSGKEVHAQIVKS 7
           L AC++V A+ +GKE+HAQI+KS
Sbjct: 351 LPACSRVAALLTGKEIHAQILKS 373



 Score =  104 bits (259), Expect = 2e-20
 Identities = 63/226 (27%), Positives = 111/226 (49%)
 Frame = -2

Query: 738 SLNEPNIDAYSSFLHSCISKKSLHHGQRLYLQLLLSKNKTHQNLLQNPIIKSKLITLYTV 559
           S  EP   + S  L +C+  K L  G+ ++ Q++  K K  Q      ++ + L+ LY  
Sbjct: 237 SFIEPGNFSISVALKACVDLKDLRVGRGIHAQIVKRKEKVDQ------VVYNVLLKLYME 290

Query: 558 CGEIDEARRVFDDGKSSDEAVWVAMAIGYSRKGLFRESLELYCEMLGQCVWPSNFGFSTA 379
            G  D+AR+VFD     +   W ++    S+K    E   L+ +M  + +  S    +T 
Sbjct: 291 SGLFDDARKVFDGMSERNVVTWNSLISVLSKKVRVHEMFNLFRKMQEEMIGFSWATLTTI 350

Query: 378 LKACSELGEVGIGRMVHAQIVKAGDEVDQVVFNSLLRLYNHCGCFDDVVKLFDEMPQRNV 199
           L ACS +  +  G+ +HAQI+K+ ++ D  + NSL+ +Y  CG  +   ++FD M  +++
Sbjct: 351 LPACSRVAALLTGKEIHAQILKSKEKPDVPLLNSLMDMYGKCGEVEYSRRVFDVMLTKDL 410

Query: 198 VTWNSLIDGLVKRGQLFEAFKLFRKMQVEGTGFSWVTLTTVLSACA 61
            +WN +++     G + E   LF  M   G     +T   +LS C+
Sbjct: 411 ASWNIMLNCYAINGNIEEVINLFEWMIESGVAPDGITFVALLSGCS 456



 Score = 63.5 bits (153), Expect = 5e-08
 Identities = 50/189 (26%), Positives = 85/189 (44%), Gaps = 3/189 (1%)
 Frame = -2

Query: 708 SSFLHSCISKKSLHHGQRLYLQLLLSKNKTHQNLLQNPIIKSKLITLYTVCGEIDEARRV 529
           ++ L +C    +L  G+ ++ Q+L SK K    LL +      L+ +Y  CGE++ +RRV
Sbjct: 348 TTILPACSRVAALLTGKEIHAQILKSKEKPDVPLLNS------LMDMYGKCGEVEYSRRV 401

Query: 528 FDDGKSSDEAVWVAMAIGYSRKGLFRESLELYCEMLGQCVWPSNFGFSTALKACSELGEV 349
           FD   + D A W  M   Y+  G   E + L+  M+   V P    F   L  CS+ G  
Sbjct: 402 FDVMLTKDLASWNIMLNCYAINGNIEEVINLFEWMIESGVAPDGITFVALLSGCSDTGLT 461

Query: 348 GIGRMVHAQIVKAGDEVDQVV--FNSLLRLYNHCGCFDDVVKLFDEMP-QRNVVTWNSLI 178
             G  +  + +K    V   +  +  L+ +    G   + VK+ + MP + +   W SL+
Sbjct: 462 EYGLSLFER-MKTEFRVSPALEHYACLVDILGRAGKIKEAVKVIETMPFKPSASIWGSLL 520

Query: 177 DGLVKRGQL 151
           +     G +
Sbjct: 521 NSCRLHGNV 529