BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cnidium21_contig00018474
         (2006 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]   792   0.0  
ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum ly...   790   0.0  
gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana ...   786   0.0  
gb|ACE06982.1| obtusifoliol-14alpha-demethylase [Gossypium hirsu...   783   0.0  
ref|XP_002299356.1| cytochrome P450 obtusifoliol 14-alpha-demeth...   783   0.0  

>gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]
          Length = 487

 Score =  792 bits (2045), Expect = 0.0
 Identities = 385/484 (79%), Positives = 436/484 (90%)
 Frame = -3

Query: 1692 NTKWLNISIIFIATLFVAKLILSVGSRYNRRRSPPVVKAPIPIIGGLLRFLKGPILLLRE 1513
            + K LN+ ++ +ATL VAKLI ++    +++R PPV+KA +PI+GGL+RFLKGPI++LR+
Sbjct: 5    DNKILNVGLLLVATLLVAKLISALIMPRSKKRLPPVIKA-LPIVGGLIRFLKGPIVMLRQ 63

Query: 1512 EYPKLGSVFTLNLVNKNITFLIGPEVSSHFFKAVETDLSQQEVYRFNVPTFGPGVVFDVD 1333
            EYPKLGSVFTLNL+NKNITF IGPEVS+HFFKA ETDLSQQEVY+FNVPTFGPGVVFDVD
Sbjct: 64   EYPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVD 123

Query: 1332 YSVRQEQFRFFTDSLRVSKLKSYVDQMVIEAQDYFSKWGDSGEVDLKHELEQLIILTASR 1153
            Y++RQEQFRFFT++LRV+KLK YVDQMV EA++YFSKWG+SGEVDLK+ELE LIILTASR
Sbjct: 124  YTIRQEQFRFFTEALRVTKLKGYVDQMVTEAEEYFSKWGESGEVDLKYELEHLIILTASR 183

Query: 1152 CLLGIEVRNKLFDDVSALFHKLDSGMLPISVIFPYLPIPAHRRRDEARKKLAEIFGRIIN 973
            CLLG EVRNKLFDDVSALFH LD+GMLPISVIFPYLPIPAHRRRD ARKKLAEIF  IIN
Sbjct: 184  CLLGEEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFANIIN 243

Query: 972  SRKEAGKSENDMLQSFMDSKYKNGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYLLA 793
            SRK  GK+ENDMLQ F+DSKYK+GRPTT+ E+TGLLIAALFAGQHTSSITSTWTG+YLL 
Sbjct: 244  SRKRTGKAENDMLQCFIDSKYKDGRPTTEGEITGLLIAALFAGQHTSSITSTWTGSYLLT 303

Query: 792  NKKYLSAVSDEQKTIMRKHGNNVDHDILSEMEALHRCIKEALRLHPPLIMLLRSSHTDFS 613
            N KY+SAV DEQK +M+KHGN VDHDILSEM+ L+RCIKEALRLHPPLIMLLRSSH+DFS
Sbjct: 304  NNKYMSAVVDEQKNLMKKHGNKVDHDILSEMDVLYRCIKEALRLHPPLIMLLRSSHSDFS 363

Query: 612  VTTREGKEYDIPKGHIVATSPAFANRLPHIYKDPDTFDPDRFAAGRDEDKAAGPFSYISF 433
            VTTREGKEYDIPKGHIVATSPAFANRLPHI+K+P+++DPDRF  GR+EDKAAG FSYISF
Sbjct: 364  VTTREGKEYDIPKGHIVATSPAFANRLPHIFKNPESYDPDRFGPGREEDKAAGAFSYISF 423

Query: 432  GGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYKCKQ 253
            GGGRHGCLGEPFAYLQIKAIWSHLLRNFE ELISPFPEIDWNA          V+YK ++
Sbjct: 424  GGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGEVMVKYKRRK 483

Query: 252  LSLE 241
            LS E
Sbjct: 484  LSAE 487


>ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum lycopersicum]
            gi|299735181|gb|ADJ37071.1| obtusifoliol
            14alpha-demethylase [Solanum lycopersicum]
          Length = 487

 Score =  790 bits (2040), Expect = 0.0
 Identities = 386/484 (79%), Positives = 435/484 (89%)
 Frame = -3

Query: 1692 NTKWLNISIIFIATLFVAKLILSVGSRYNRRRSPPVVKAPIPIIGGLLRFLKGPILLLRE 1513
            + K LN+ ++ +ATL VAKLI ++    +++R PPVVKA  PI+GGL+RFLKGP+++LR+
Sbjct: 5    DNKILNVGLLLVATLLVAKLISALIMPRSKKRLPPVVKA-WPIVGGLIRFLKGPVVMLRQ 63

Query: 1512 EYPKLGSVFTLNLVNKNITFLIGPEVSSHFFKAVETDLSQQEVYRFNVPTFGPGVVFDVD 1333
            EYPKLGSVFTLNL+NKNITF IGPEVS+HFFKA ETDLSQQEVY+FNVPTFGPGVVFDVD
Sbjct: 64   EYPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVD 123

Query: 1332 YSVRQEQFRFFTDSLRVSKLKSYVDQMVIEAQDYFSKWGDSGEVDLKHELEQLIILTASR 1153
            Y++RQEQFRFFT++LRV+KLK YVDQMV EA++YFSKWG+SGEVDLK+ELE LIILTASR
Sbjct: 124  YTIRQEQFRFFTEALRVTKLKGYVDQMVTEAEEYFSKWGESGEVDLKYELEHLIILTASR 183

Query: 1152 CLLGIEVRNKLFDDVSALFHKLDSGMLPISVIFPYLPIPAHRRRDEARKKLAEIFGRIIN 973
            CLLG EVRNKLFDDVSALFH LD+GMLPISVIFPYLPIPAHRRRD ARKKLAEIF  IIN
Sbjct: 184  CLLGEEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFANIIN 243

Query: 972  SRKEAGKSENDMLQSFMDSKYKNGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYLLA 793
            SRK  GK+ENDMLQ F+DSKYK+GRPTT+ E+TGLLIAALFAGQHTSSITSTWTG+YLL 
Sbjct: 244  SRKRTGKAENDMLQCFIDSKYKDGRPTTEGEITGLLIAALFAGQHTSSITSTWTGSYLLT 303

Query: 792  NKKYLSAVSDEQKTIMRKHGNNVDHDILSEMEALHRCIKEALRLHPPLIMLLRSSHTDFS 613
            N KY+SAV DEQK +M+KHGN VDHDILSEME L+RCIKEALRLHPPLIMLLRSSH++FS
Sbjct: 304  NDKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSEFS 363

Query: 612  VTTREGKEYDIPKGHIVATSPAFANRLPHIYKDPDTFDPDRFAAGRDEDKAAGPFSYISF 433
            VTTREGKEYDI KGHIVATSPAFANRLPHI+K+PDT+DPDRF  GR+EDKAAG FSYISF
Sbjct: 364  VTTREGKEYDISKGHIVATSPAFANRLPHIFKNPDTYDPDRFGPGREEDKAAGAFSYISF 423

Query: 432  GGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYKCKQ 253
            GGGRHGCLGEPFAYLQIKAIWSHLLRNFE ELISPFPEIDWNA          V+YK ++
Sbjct: 424  GGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGEVMVKYKRRK 483

Query: 252  LSLE 241
            LS+E
Sbjct: 484  LSVE 487


>gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana tabacum]
          Length = 487

 Score =  786 bits (2031), Expect = 0.0
 Identities = 384/484 (79%), Positives = 433/484 (89%)
 Frame = -3

Query: 1692 NTKWLNISIIFIATLFVAKLILSVGSRYNRRRSPPVVKAPIPIIGGLLRFLKGPILLLRE 1513
            + K LN  ++ +ATL VAKLI ++    +++R PPV+K+  PI+GGLLRFLKGP+++LRE
Sbjct: 5    DNKILNAGLLLVATLVVAKLISALIMPRSKKRLPPVIKS-WPILGGLLRFLKGPVVMLRE 63

Query: 1512 EYPKLGSVFTLNLVNKNITFLIGPEVSSHFFKAVETDLSQQEVYRFNVPTFGPGVVFDVD 1333
            EYPKLGSVFTLNL+NKNITF +GPEVS+HFFKA ETDLSQQEVY+FNVPTFGPGVVFDVD
Sbjct: 64   EYPKLGSVFTLNLLNKNITFFVGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVD 123

Query: 1332 YSVRQEQFRFFTDSLRVSKLKSYVDQMVIEAQDYFSKWGDSGEVDLKHELEQLIILTASR 1153
            Y++RQEQFRFFT+SLRV+KLK YVDQMV+EA++YFSKWGDSGEVDLK+ELE LIILTASR
Sbjct: 124  YTIRQEQFRFFTESLRVNKLKGYVDQMVMEAEEYFSKWGDSGEVDLKYELEHLIILTASR 183

Query: 1152 CLLGIEVRNKLFDDVSALFHKLDSGMLPISVIFPYLPIPAHRRRDEARKKLAEIFGRIIN 973
            CLLG EVRNKLF+DVSALFH LD+GMLPISVIFPYLPIPAHRRRD ARKKLAEIF  II+
Sbjct: 184  CLLGEEVRNKLFEDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFANIID 243

Query: 972  SRKEAGKSENDMLQSFMDSKYKNGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYLLA 793
            SRK  GK+E+DMLQ F+DSKYK+GR TT++E+TGLLIAALFAGQHTSSITSTW GAYLL 
Sbjct: 244  SRKRTGKAESDMLQCFIDSKYKDGRATTESEITGLLIAALFAGQHTSSITSTWAGAYLLC 303

Query: 792  NKKYLSAVSDEQKTIMRKHGNNVDHDILSEMEALHRCIKEALRLHPPLIMLLRSSHTDFS 613
            N KY+SAV DEQK +M+KHGN VDHDILSEME L+RCIKEALRLHPPLIMLLRSSH+DF+
Sbjct: 304  NNKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDFT 363

Query: 612  VTTREGKEYDIPKGHIVATSPAFANRLPHIYKDPDTFDPDRFAAGRDEDKAAGPFSYISF 433
            V TREGKEYDIPKGHIVATSPAFANRLPH+YK+PDT+DPDRFA GRDEDK AG FSYISF
Sbjct: 364  VKTREGKEYDIPKGHIVATSPAFANRLPHVYKNPDTYDPDRFAPGRDEDKVAGAFSYISF 423

Query: 432  GGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYKCKQ 253
            GGGRHGCLGEPFAYLQIKAIWSHLLRNFE ELISPFPEIDWNA          V+YK ++
Sbjct: 424  GGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGKVMVKYKRRK 483

Query: 252  LSLE 241
            LS E
Sbjct: 484  LSTE 487


>gb|ACE06982.1| obtusifoliol-14alpha-demethylase [Gossypium hirsutum]
          Length = 486

 Score =  783 bits (2023), Expect = 0.0
 Identities = 389/483 (80%), Positives = 430/483 (89%)
 Frame = -3

Query: 1692 NTKWLNISIIFIATLFVAKLILSVGSRYNRRRSPPVVKAPIPIIGGLLRFLKGPILLLRE 1513
            N K LN  I+ +ATL VAKLI  +    +++R PP VK   P+IGGLLRF+KGPI++LRE
Sbjct: 4    NDKLLNTGILIVATLVVAKLISFLIMPRSKKRVPPAVKT-WPVIGGLLRFMKGPIVMLRE 62

Query: 1512 EYPKLGSVFTLNLVNKNITFLIGPEVSSHFFKAVETDLSQQEVYRFNVPTFGPGVVFDVD 1333
            EYPKLGSVFTLNL NK ITFLIGPEVSSHFFKA E+DLSQQEVY+FNVPTFGPGVVFDVD
Sbjct: 63   EYPKLGSVFTLNLFNKKITFLIGPEVSSHFFKASESDLSQQEVYQFNVPTFGPGVVFDVD 122

Query: 1332 YSVRQEQFRFFTDSLRVSKLKSYVDQMVIEAQDYFSKWGDSGEVDLKHELEQLIILTASR 1153
            Y++RQEQFRFFT++LRV+KLK YVDQMV EA+DYFSKWGDSGEVDLK+ELE LIILTASR
Sbjct: 123  YTIRQEQFRFFTEALRVNKLKGYVDQMVTEAEDYFSKWGDSGEVDLKYELEHLIILTASR 182

Query: 1152 CLLGIEVRNKLFDDVSALFHKLDSGMLPISVIFPYLPIPAHRRRDEARKKLAEIFGRIIN 973
            CLLG EVRNKLFDDVSALFH LD+GMLPISVIFPYLPIPAHRRRD ARKKLA IF  II 
Sbjct: 183  CLLGQEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDRARKKLAGIFANIIA 242

Query: 972  SRKEAGKSENDMLQSFMDSKYKNGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYLLA 793
            SRK AGKSENDMLQ F++SKYK+GRPTT+ EVTGLLIAALFAGQHTSSITSTWTGAYLL 
Sbjct: 243  SRKSAGKSENDMLQCFIESKYKDGRPTTEAEVTGLLIAALFAGQHTSSITSTWTGAYLLR 302

Query: 792  NKKYLSAVSDEQKTIMRKHGNNVDHDILSEMEALHRCIKEALRLHPPLIMLLRSSHTDFS 613
            +K++LSAV +EQK IM+KHG+ VDHDILSEM+ L+RCIKEALRLHPPLIMLLRSSH+DFS
Sbjct: 303  HKEFLSAVIEEQKQIMQKHGSKVDHDILSEMDTLYRCIKEALRLHPPLIMLLRSSHSDFS 362

Query: 612  VTTREGKEYDIPKGHIVATSPAFANRLPHIYKDPDTFDPDRFAAGRDEDKAAGPFSYISF 433
            V TR+GKEYDIPKGHIVATSPAFANRLP+IYKDPDT+DPDRF+ GR+EDK AG FSYISF
Sbjct: 363  VKTRDGKEYDIPKGHIVATSPAFANRLPYIYKDPDTYDPDRFSVGREEDKVAGAFSYISF 422

Query: 432  GGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYKCKQ 253
            GGGRHGCLGEPFAYLQIKAIWSHLLRNFELEL+SPFPEIDWNA          VRYK +Q
Sbjct: 423  GGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGKVMVRYKRRQ 482

Query: 252  LSL 244
            LS+
Sbjct: 483  LSV 485


>ref|XP_002299356.1| cytochrome P450 obtusifoliol 14-alpha-demethylase [Populus
            trichocarpa] gi|222846614|gb|EEE84161.1| cytochrome P450
            obtusifoliol 14-alpha-demethylase [Populus trichocarpa]
          Length = 488

 Score =  783 bits (2022), Expect = 0.0
 Identities = 382/483 (79%), Positives = 436/483 (90%)
 Frame = -3

Query: 1692 NTKWLNISIIFIATLFVAKLILSVGSRYNRRRSPPVVKAPIPIIGGLLRFLKGPILLLRE 1513
            + K+LN+ ++ IATL VAKLI ++    +++R PPV+K   P+IGGL+RFLKGPI++LRE
Sbjct: 6    DNKFLNVGLLIIATLLVAKLISALIMPRSQKRLPPVMKG-WPLIGGLIRFLKGPIVMLRE 64

Query: 1512 EYPKLGSVFTLNLVNKNITFLIGPEVSSHFFKAVETDLSQQEVYRFNVPTFGPGVVFDVD 1333
            EYPKLGSVFT+NLVN+ ITFLIGPEVS+HFFKA E DLSQQEVY+FNVPTFGPGVVFDV+
Sbjct: 65   EYPKLGSVFTVNLVNRKITFLIGPEVSAHFFKASEVDLSQQEVYQFNVPTFGPGVVFDVE 124

Query: 1332 YSVRQEQFRFFTDSLRVSKLKSYVDQMVIEAQDYFSKWGDSGEVDLKHELEQLIILTASR 1153
            YS+RQEQFRFFT++LRV+KLK YVDQMV+EA+DYF KWGDSG VDLK+ELE LIILTASR
Sbjct: 125  YSIRQEQFRFFTEALRVNKLKGYVDQMVVEAEDYFLKWGDSGVVDLKYELEHLIILTASR 184

Query: 1152 CLLGIEVRNKLFDDVSALFHKLDSGMLPISVIFPYLPIPAHRRRDEARKKLAEIFGRIIN 973
            CLLG EVR+KLFDDV+ALFH LD+GMLP+SV+FPYLPIPAHRRRD ARKKLAEIF  IIN
Sbjct: 185  CLLGREVRDKLFDDVAALFHDLDNGMLPVSVLFPYLPIPAHRRRDRARKKLAEIFANIIN 244

Query: 972  SRKEAGKSENDMLQSFMDSKYKNGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYLLA 793
            SRK A KSENDMLQ F+DSKYK+GRPTT++E+TGLLIAALFAGQHTSSITSTWTGAYLL 
Sbjct: 245  SRKLASKSENDMLQCFIDSKYKDGRPTTESEITGLLIAALFAGQHTSSITSTWTGAYLLR 304

Query: 792  NKKYLSAVSDEQKTIMRKHGNNVDHDILSEMEALHRCIKEALRLHPPLIMLLRSSHTDFS 613
            + +YLSAV +EQK +M+KHGN VDHDILSEM+ L+RCIKEALRLHPPLIMLLRSSH+DFS
Sbjct: 305  HNEYLSAVLEEQKNLMKKHGNKVDHDILSEMDVLYRCIKEALRLHPPLIMLLRSSHSDFS 364

Query: 612  VTTREGKEYDIPKGHIVATSPAFANRLPHIYKDPDTFDPDRFAAGRDEDKAAGPFSYISF 433
            VTTR+GKEYDIPKGHIVATSPAFANRLPH++KDPD++DPDRFA GR+EDKAAG FSYISF
Sbjct: 365  VTTRDGKEYDIPKGHIVATSPAFANRLPHVFKDPDSYDPDRFAYGREEDKAAGAFSYISF 424

Query: 432  GGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYKCKQ 253
            GGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNA          VRYK ++
Sbjct: 425  GGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAMVVGVKDKVMVRYKRRE 484

Query: 252  LSL 244
            LS+
Sbjct: 485  LSV 487