BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cnidium21_contig00008051
         (1530 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q40588.1|ASO_TOBAC RecName: Full=L-ascorbate oxidase; Short=A...   537   0.0  
ref|XP_002275678.1| PREDICTED: L-ascorbate oxidase-like [Vitis v...   526   0.0  
emb|CAN82127.1| hypothetical protein VITISV_044128 [Vitis vinifera]   526   0.0  
ref|XP_002312838.1| l-ascorbate oxidase precursor [Populus trich...   521   0.0  
gb|ABK96191.1| unknown [Populus trichocarpa]                          513   e-178

>sp|Q40588.1|ASO_TOBAC RecName: Full=L-ascorbate oxidase; Short=ASO; Short=Ascorbase; Flags:
            Precursor gi|599594|dbj|BAA07734.1| ascorbate oxidase
            precursor [Nicotiana tabacum]
          Length = 578

 Score =  537 bits (1384), Expect(2) = 0.0
 Identities = 254/370 (68%), Positives = 290/370 (78%)
 Frame = -3

Query: 1246 LAAQFSNPPLDQCKFTGTEQCAPQTMTVEPNKIYRLRIXXXXXXXXXXXAIGNHKMVVVE 1067
            LAA+FS PPL QCK  G EQ APQ + V PNKIYRLR+           AIG HKMVVVE
Sbjct: 209  LAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLRVASTTALGSLSLAIGGHKMVVVE 268

Query: 1066 ADGNYVQPFSVYDMDIYSGESYSILFKADQSPAQNYWISVGVRGRKPNTPQALTILHYXX 887
            ADGNYVQPFSV DMDIYSGESYS+LFK DQ P +NYWIS+ VRGR+P TPQ LT+L+Y  
Sbjct: 269  ADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYWISINVRGREPKTPQGLTLLNYLP 328

Query: 886  XXXXXXXXXXXXXXXLWNDYDHSKSFTNRIKALRGSPNPPTNYNRRIMLLNTQNKMNGFT 707
                           LWNDY+HSKSF+N+I AL GSP PP   +RRI+LLNTQNK++G+T
Sbjct: 329  NSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSPKPPPQNHRRIILLNTQNKIDGYT 388

Query: 706  KWAINNISLSLPVTPYLGSIKYNLYKAFANSPPPETFSPVYDVMRPPPNPNTTQGDGIYM 527
            KWAINN+SL LP   YLGSI+Y +  AF   PPP+ F   YDV++  PN N+T G+G+YM
Sbjct: 389  KWAINNVSLVLPTQLYLGSIRYGI-NAFDTKPPPDNFPKDYDVLKQAPNSNSTYGNGVYM 447

Query: 526  LKFNSTIDVILQNANALADNVSEIHPWHLHGHDFWVLGYGEEKFTDKDVVKFNLKNPPLR 347
            LKFN+TID+ILQNANALA +VSEIHPWHLHGHDFWVLGYGE KF++KDV KFNLKNPPLR
Sbjct: 448  LKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWVLGYGEGKFSEKDVKKFNLKNPPLR 507

Query: 346  NSAVIFPYGWTALRFVADNPGAWAFHCHIEPHLHMGMGVVFAEGIYRVKNIPKEALTCGL 167
            N+AVIFP+GWTALRFV DNPG WAFHCHIEPHLHMGMGV+FAEG++ VK IPKEAL CGL
Sbjct: 508  NTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVHLVKKIPKEALACGL 567

Query: 166  TGKMFMDKKH 137
            TGKM M  KH
Sbjct: 568  TGKMLMSNKH 577



 Score =  151 bits (382), Expect(2) = 0.0
 Identities = 67/77 (87%), Positives = 72/77 (93%)
 Frame = -1

Query: 1530 GHYGMQRSAGLYGSLIVDVAENVKEPFRYDGEFNLLLSDWWHKNAHEQELDLSSNPMRWI 1351
            GHYGMQRSAGLYGSLIV+V E  KEPF YDGEFNLLLSDWWHK +HEQE+DLSSNP+RWI
Sbjct: 132  GHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSSNPLRWI 191

Query: 1350 GEPQSLLLNGRGQYNCS 1300
            GEPQ+LLLNGRGQYNCS
Sbjct: 192  GEPQTLLLNGRGQYNCS 208


>ref|XP_002275678.1| PREDICTED: L-ascorbate oxidase-like [Vitis vinifera]
          Length = 581

 Score =  526 bits (1354), Expect(2) = 0.0
 Identities = 245/366 (66%), Positives = 284/366 (77%)
 Frame = -3

Query: 1246 LAAQFSNPPLDQCKFTGTEQCAPQTMTVEPNKIYRLRIXXXXXXXXXXXAIGNHKMVVVE 1067
            LAA  +N    QC+F G EQC+PQ + V+P+K YRLR+            IGNHKMV+VE
Sbjct: 210  LAAHVTNSSSPQCQFRGNEQCSPQILHVQPHKTYRLRVSSTTALASLNLQIGNHKMVMVE 269

Query: 1066 ADGNYVQPFSVYDMDIYSGESYSILFKADQSPAQNYWISVGVRGRKPNTPQALTILHYXX 887
            ADGNY+QP +V D+DIYSGESYS+L   DQ P++NYWISV VRGR+P TPQ LTIL+Y  
Sbjct: 270  ADGNYIQPVAVDDLDIYSGESYSVLIHTDQDPSKNYWISVSVRGREPKTPQGLTILNYQT 329

Query: 886  XXXXXXXXXXXXXXXLWNDYDHSKSFTNRIKALRGSPNPPTNYNRRIMLLNTQNKMNGFT 707
                           LWNDY+HSKSF+N+I AL GSP PPT YNRRI+LLNTQN +NGFT
Sbjct: 330  TSASKLPTSTPPVSPLWNDYNHSKSFSNKILALMGSPKPPTTYNRRIILLNTQNTINGFT 389

Query: 706  KWAINNISLSLPVTPYLGSIKYNLYKAFANSPPPETFSPVYDVMRPPPNPNTTQGDGIYM 527
            KWAINNISL+LP TPYLG+IK+ L  AF    PPE F   YDVM+PP N N+T G+G+YM
Sbjct: 390  KWAINNISLTLPPTPYLGAIKHRLSNAFDQKSPPENFPNDYDVMKPPTNTNSTYGNGVYM 449

Query: 526  LKFNSTIDVILQNANALADNVSEIHPWHLHGHDFWVLGYGEEKFTDKDVVKFNLKNPPLR 347
            L+F +T+DVILQNANALA  VSEIHPWHLHGHDFWVLGYGE KF +KD  +FNLKNPPLR
Sbjct: 450  LEFRTTVDVILQNANALATGVSEIHPWHLHGHDFWVLGYGEGKFREKDAKRFNLKNPPLR 509

Query: 346  NSAVIFPYGWTALRFVADNPGAWAFHCHIEPHLHMGMGVVFAEGIYRVKNIPKEALTCGL 167
            N+AVIFP+GWTALRFVADNPG WAFHCHIEPHLHMGMGVVFAEG++ VK++P  AL CGL
Sbjct: 510  NTAVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHLVKDVPNHALACGL 569

Query: 166  TGKMFM 149
            TGKM M
Sbjct: 570  TGKMLM 575



 Score =  141 bits (355), Expect(2) = 0.0
 Identities = 62/77 (80%), Positives = 68/77 (88%)
 Frame = -1

Query: 1530 GHYGMQRSAGLYGSLIVDVAENVKEPFRYDGEFNLLLSDWWHKNAHEQELDLSSNPMRWI 1351
            GHYGMQRSAGLYGSL+V+V E  KEPF YDGEFNLLLSDWWHK + EQE+ LSS P RWI
Sbjct: 133  GHYGMQRSAGLYGSLVVEVGEGQKEPFHYDGEFNLLLSDWWHKGSQEQEVALSSKPFRWI 192

Query: 1350 GEPQSLLLNGRGQYNCS 1300
            GEPQ+LL+NGRGQYNCS
Sbjct: 193  GEPQTLLINGRGQYNCS 209


>emb|CAN82127.1| hypothetical protein VITISV_044128 [Vitis vinifera]
          Length = 558

 Score =  526 bits (1355), Expect(2) = 0.0
 Identities = 246/366 (67%), Positives = 283/366 (77%)
 Frame = -3

Query: 1246 LAAQFSNPPLDQCKFTGTEQCAPQTMTVEPNKIYRLRIXXXXXXXXXXXAIGNHKMVVVE 1067
            LAA  +N    QC+F G EQCAPQ + V+P+K YRLR+            IGNHKMV+VE
Sbjct: 187  LAAHVTNSSSPQCQFRGNEQCAPQILHVQPHKTYRLRVSSTTALASLNLQIGNHKMVMVE 246

Query: 1066 ADGNYVQPFSVYDMDIYSGESYSILFKADQSPAQNYWISVGVRGRKPNTPQALTILHYXX 887
            ADGNY+QP +V D+DIYSGESYS+L   DQ P+ NYWISV VRGR+P TPQ LTIL+Y  
Sbjct: 247  ADGNYIQPVAVDDLDIYSGESYSVLIHTDQDPSXNYWISVSVRGREPKTPQGLTILNYQT 306

Query: 886  XXXXXXXXXXXXXXXLWNDYDHSKSFTNRIKALRGSPNPPTNYNRRIMLLNTQNKMNGFT 707
                           LWNDY+HSKSF+N+I AL GSP PPT YNRRI+LLNTQN +NGFT
Sbjct: 307  TSASKLPTSTPPVSPLWNDYNHSKSFSNKILALMGSPKPPTTYNRRIILLNTQNTINGFT 366

Query: 706  KWAINNISLSLPVTPYLGSIKYNLYKAFANSPPPETFSPVYDVMRPPPNPNTTQGDGIYM 527
            KWAINNISL+LP TPYLG+IK+ L  AF    PPE F   YDVM+PP N N+T G+G+YM
Sbjct: 367  KWAINNISLTLPPTPYLGAIKHRLSNAFDQKSPPENFPNDYDVMKPPTNTNSTYGNGVYM 426

Query: 526  LKFNSTIDVILQNANALADNVSEIHPWHLHGHDFWVLGYGEEKFTDKDVVKFNLKNPPLR 347
            L+F +T+DVILQNANALA  VSEIHPWHLHGHDFWVLGYGE KF +KD  +FNLKNPPLR
Sbjct: 427  LEFRTTVDVILQNANALATGVSEIHPWHLHGHDFWVLGYGEGKFREKDAKRFNLKNPPLR 486

Query: 346  NSAVIFPYGWTALRFVADNPGAWAFHCHIEPHLHMGMGVVFAEGIYRVKNIPKEALTCGL 167
            N+AVIFP+GWTALRFVADNPG WAFHCHIEPHLHMGMGVVFAEG++ VK++P  AL CGL
Sbjct: 487  NTAVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHLVKDVPNHALACGL 546

Query: 166  TGKMFM 149
            TGKM M
Sbjct: 547  TGKMLM 552



 Score =  139 bits (351), Expect(2) = 0.0
 Identities = 62/77 (80%), Positives = 67/77 (87%)
 Frame = -1

Query: 1530 GHYGMQRSAGLYGSLIVDVAENVKEPFRYDGEFNLLLSDWWHKNAHEQELDLSSNPMRWI 1351
            GHYGMQRSAGLYGSL V+V E  KEPF YDGEFNLLLSDWWHK + EQE+ LSS P RWI
Sbjct: 110  GHYGMQRSAGLYGSLXVEVGEGQKEPFHYDGEFNLLLSDWWHKGSQEQEVALSSKPFRWI 169

Query: 1350 GEPQSLLLNGRGQYNCS 1300
            GEPQ+LL+NGRGQYNCS
Sbjct: 170  GEPQTLLINGRGQYNCS 186


>ref|XP_002312838.1| l-ascorbate oxidase precursor [Populus trichocarpa]
            gi|222849246|gb|EEE86793.1| l-ascorbate oxidase precursor
            [Populus trichocarpa]
          Length = 597

 Score =  521 bits (1343), Expect(2) = 0.0
 Identities = 248/369 (67%), Positives = 284/369 (76%), Gaps = 3/369 (0%)
 Frame = -3

Query: 1246 LAAQFSN-PPLDQCKFTGTEQCAPQTMTVEPNKIYRLRIXXXXXXXXXXXAIGNHKMVVV 1070
            LAA +SN   L QC  TG EQ AP  + V+PNK YR+R+           AIGNHKM+VV
Sbjct: 223  LAAHYSNNSSLSQCNVTGHEQWAPYILHVDPNKTYRIRLSSTTALASLNLAIGNHKMLVV 282

Query: 1069 EADGNYVQPFSVYDMDIYSGESYSILFKADQSPAQNYWISVGVRGRKPNTPQALTILHYX 890
            EADGNY+QPF   D+DIYSGESYS+L K  Q P+QNYWIS GVRGRKP TPQALTIL+Y 
Sbjct: 283  EADGNYLQPFETDDLDIYSGESYSVLLKTSQDPSQNYWISFGVRGRKPQTPQALTILNYK 342

Query: 889  XXXXXXXXXXXXXXXXLWNDYDHSKSFTNRIKAL--RGSPNPPTNYNRRIMLLNTQNKMN 716
                             W+DY HSK+FTN++KAL  +  P PP+ Y+RRI+LLNTQNKMN
Sbjct: 343  TNSASKFPLSPPPVTPRWDDYAHSKAFTNKVKALDHKTIPKPPSTYHRRIILLNTQNKMN 402

Query: 715  GFTKWAINNISLSLPVTPYLGSIKYNLYKAFANSPPPETFSPVYDVMRPPPNPNTTQGDG 536
            G+TKW+INN+SLSLP TPYLGSI++ L   F  + PPE+F   YDVM+PP NPNTT G+G
Sbjct: 403  GYTKWSINNVSLSLPATPYLGSIRFGLQNGFDQTKPPESFPVQYDVMKPPGNPNTTTGNG 462

Query: 535  IYMLKFNSTIDVILQNANALADNVSEIHPWHLHGHDFWVLGYGEEKFTDKDVVKFNLKNP 356
            +YML + ST+DVILQNANALA+NVSEIHPWHLHGHDFWVLGYGE KFT  D  KFN+KNP
Sbjct: 463  VYMLSYYSTVDVILQNANALAENVSEIHPWHLHGHDFWVLGYGEGKFTKDDEKKFNMKNP 522

Query: 355  PLRNSAVIFPYGWTALRFVADNPGAWAFHCHIEPHLHMGMGVVFAEGIYRVKNIPKEALT 176
            P RNSAVIFPYGWTALRFVADNPG WAFHCHIEPHLHMGMGVV AEG+ R+  IPKEAL+
Sbjct: 523  PYRNSAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVLAEGVQRLPKIPKEALS 582

Query: 175  CGLTGKMFM 149
            CGLTGK FM
Sbjct: 583  CGLTGKKFM 591



 Score =  143 bits (361), Expect(2) = 0.0
 Identities = 64/77 (83%), Positives = 70/77 (90%)
 Frame = -1

Query: 1530 GHYGMQRSAGLYGSLIVDVAENVKEPFRYDGEFNLLLSDWWHKNAHEQELDLSSNPMRWI 1351
            GHYGMQRSAGLYGSLIVDVAE  KEPF YDGEF+LLLSDWWH++AH QE+ LSS PMRWI
Sbjct: 146  GHYGMQRSAGLYGSLIVDVAEGEKEPFHYDGEFDLLLSDWWHESAHHQEVGLSSRPMRWI 205

Query: 1350 GEPQSLLLNGRGQYNCS 1300
            GEPQ+LL+NGRGQY CS
Sbjct: 206  GEPQTLLVNGRGQYGCS 222


>gb|ABK96191.1| unknown [Populus trichocarpa]
          Length = 594

 Score =  513 bits (1321), Expect(2) = e-178
 Identities = 244/372 (65%), Positives = 278/372 (74%), Gaps = 2/372 (0%)
 Frame = -3

Query: 1246 LAAQFSNPPLDQCKFTGTEQCAPQTMTVEPNKIYRLRIXXXXXXXXXXXAIGNHKMVVVE 1067
            LAA   N    QC  TG EQ AP  + V+PNK YR+RI           AIGNHKM+VVE
Sbjct: 221  LAAHHGNSSFSQCNITGHEQWAPYILHVDPNKTYRIRIASTTALASLNLAIGNHKMMVVE 280

Query: 1066 ADGNYVQPFSVYDMDIYSGESYSILFKADQSPAQNYWISVGVRGRKPNTPQALTILHYXX 887
            ADGNY+QPF   D+DIYSGESYS+L K  Q  +QNYWIS GVRGR+P+TPQALTIL+Y  
Sbjct: 281  ADGNYLQPFETNDLDIYSGESYSVLLKTSQDSSQNYWISFGVRGREPHTPQALTILNYKT 340

Query: 886  XXXXXXXXXXXXXXXLWNDYDHSKSFTNRIKAL--RGSPNPPTNYNRRIMLLNTQNKMNG 713
                            W+DY HSK+F+N+IKAL  +  P P   Y+RRI+LLNTQNKMNG
Sbjct: 341  NSASKLPLSPPPVTPRWDDYAHSKAFSNKIKALDHKSIPKPSPTYHRRIVLLNTQNKMNG 400

Query: 712  FTKWAINNISLSLPVTPYLGSIKYNLYKAFANSPPPETFSPVYDVMRPPPNPNTTQGDGI 533
            +TKW+INN+SLS+P TPYLGSI++ L  AF  + PPE F   YDVM+PP NPNTT G G+
Sbjct: 401  YTKWSINNVSLSIPATPYLGSIRFGLRDAFDQTKPPEDFPGHYDVMKPPVNPNTTTGSGV 460

Query: 532  YMLKFNSTIDVILQNANALADNVSEIHPWHLHGHDFWVLGYGEEKFTDKDVVKFNLKNPP 353
            YML + ST+DVILQNANAL +NVSEIHPWHLHGHDFWVLGYGE KFT  D  KFNLKNPP
Sbjct: 461  YMLGYYSTVDVILQNANALTENVSEIHPWHLHGHDFWVLGYGEGKFTKSDEKKFNLKNPP 520

Query: 352  LRNSAVIFPYGWTALRFVADNPGAWAFHCHIEPHLHMGMGVVFAEGIYRVKNIPKEALTC 173
             RN+AVIFPYGWTALRFVADNPG WAFHCHIEPHLH+GMGVVFAEG+  V  IPKEAL+C
Sbjct: 521  YRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHLGMGVVFAEGVQHVAKIPKEALSC 580

Query: 172  GLTGKMFMDKKH 137
            GLTGK FM   H
Sbjct: 581  GLTGKRFMTGSH 592



 Score =  140 bits (354), Expect(2) = e-178
 Identities = 62/77 (80%), Positives = 68/77 (88%)
 Frame = -1

Query: 1530 GHYGMQRSAGLYGSLIVDVAENVKEPFRYDGEFNLLLSDWWHKNAHEQELDLSSNPMRWI 1351
            GHYGMQRSAGLYGS+IVDVAE  KEPF YDGEF+LLLSDWWHK+ H QE+ LSS PMRWI
Sbjct: 144  GHYGMQRSAGLYGSMIVDVAEGEKEPFHYDGEFDLLLSDWWHKSVHHQEVGLSSRPMRWI 203

Query: 1350 GEPQSLLLNGRGQYNCS 1300
             EPQ+LL+NGRGQY CS
Sbjct: 204  NEPQALLINGRGQYGCS 220